Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.585
B. Dheeb, S. Hashim, Rawad Kh. Hameed, Shilan W. Hasan
Background: Prosopis fracta is a valuable and useful medicinal plant for the purpose of extracting flavonoids and it is one of the most important plants with medicinal properties. Prosopis fracta is found in many southern regions of Iran. Objective: the aim of this review Phytochemistry and biological activity of Prosopis fracta. Material and Methods: Initially, the powder was degreased by hexane and then after completion of the extraction process, we obtained a rotary solvent removal and the rest of the extract was frozen for retention, then it stored at -23°C. The freeze-dried samples are dissolved in methanol and permanently filtered through a 0.35 syringe filter. Results: Prosopis fracta extracts using to treat cancer, which destroying activity via mitochondrial changes, as showed a good example for increased reactive oxygen species (ROS) levels, SIRT3 activity and cell death in HT-29 related to the intestines and rectum cancer cells. Conclusion: Antimicrobial and phytochemical studies provide valuable insight into understanding media. Sometimes the idea of the route responsible for the activity of these phytochemicals against oxidizing compounds that act as a source of harmful chemical to the body is removal from service of protein and loss of function.
{"title":"Phytochemistry and biological activity of Prosopis fracta","authors":"B. Dheeb, S. Hashim, Rawad Kh. Hameed, Shilan W. Hasan","doi":"10.24126/jobrc.2020.14.1.585","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.585","url":null,"abstract":"Background: Prosopis fracta is a valuable and useful medicinal plant for the purpose of extracting flavonoids and it is one of the most important plants with medicinal properties. Prosopis fracta is found in many southern regions of Iran. \u0000Objective: the aim of this review Phytochemistry and biological activity of Prosopis fracta. \u0000Material and Methods: Initially, the powder was degreased by hexane and then after completion of the extraction process, we obtained a rotary solvent removal and the rest of the extract was frozen for retention, then it stored at -23°C. The freeze-dried samples are dissolved in methanol and permanently filtered through a 0.35 syringe filter. \u0000Results: Prosopis fracta extracts using to treat cancer, which destroying activity via mitochondrial changes, as showed a good example for increased reactive oxygen species (ROS) levels, SIRT3 activity and cell death in HT-29 related to the intestines and rectum cancer cells. \u0000Conclusion: Antimicrobial and phytochemical studies provide valuable insight into understanding media. Sometimes the idea of the route responsible for the activity of these phytochemicals against oxidizing compounds that act as a source of harmful chemical to the body is removal from service of protein and loss of function. \u0000 ","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73305489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.583
Rayhana Saad Najim, Zainab Yaseen M. Hasan, Rawa'a Al-Chalabi
Background: medicinal plants are abundant in phytochemicals, which represent the richest bioresource of drugs are used against various diseases. Objective: The present study included an in-vitro antimicrobial investigation for one of wild Iraqi plant Lepidium draba on some skin infectious agents. Methods: The fresh aerial plant parts were macerated in 80% ethanol and subjected to phytochemical general test to investigate the plant active contents. Totat flavonoids were isolated through plant reflex in acidic aqeous solution to obtain the aglycone flavonoids using ethylacetate as an organic solvent. Qualification and quantification of the isolated total flavonoids were done in coressponding to standard s flavonoids. An antimicrobial activity for the crud ethanolic extract and the isolated total flavonoids had been carried out on some skin infectous agents using the following strain: Staph aureus, Pseudomoneus aerogenosa and Candida albicans. Results: the outcome showed that the plant contain major active constituent included flavonoids Tannins, polysaccharides, alkaloids,saponines and Polyphenolic compounds.The plant contains many types of flavonoids including Rutin, Qurecetin, Kampferol and Luteolin, and each 100 g fresh aerial parts will contain 28 mg total flavonoids. The amount of each type of flavonoids were detected by HPLC technique. the extracted flavonoids at concentation of 4mg/ml showed potent effect upon the gram bacteria negative pseudomonas aeruginosa which is known to be more virulence than the gram positive strains but has no effect on Staphylococcus aureus. Also the extracted flavonoids appeared to be affected against the Candida albicans growth. Conclusion: the ethanolic extractv of locally plant L.draba is effecient to inhibit the growth of pathogenic bacteria and decrease the chances of skin infection that provided the justification for therapeutic potential as supplementary or alternative medicine.
{"title":"Study The Antimicrobial Activity of Ethanolic Extract of Lepidium draba on Some Skin Infectious Agents","authors":"Rayhana Saad Najim, Zainab Yaseen M. Hasan, Rawa'a Al-Chalabi","doi":"10.24126/jobrc.2020.14.1.583","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.583","url":null,"abstract":"Background: medicinal plants are abundant in phytochemicals, which represent the richest bioresource of drugs are used against various diseases. \u0000Objective: The present study included an in-vitro antimicrobial investigation for one of wild Iraqi plant Lepidium draba on some skin infectious agents. \u0000Methods: The fresh aerial plant parts were macerated in 80% ethanol and subjected to phytochemical general test to investigate the plant active contents. Totat flavonoids were isolated through plant reflex in acidic aqeous solution to obtain the aglycone flavonoids using ethylacetate as an organic solvent. Qualification and quantification of the isolated total flavonoids were done in coressponding to standard s flavonoids. An antimicrobial activity for the crud ethanolic extract and the isolated total flavonoids had been carried out on some skin infectous agents using the following strain: Staph aureus, Pseudomoneus aerogenosa and Candida albicans. \u0000Results: the outcome showed that the plant contain major active constituent included flavonoids Tannins, polysaccharides, alkaloids,saponines and Polyphenolic compounds.The plant contains many types of flavonoids including Rutin, Qurecetin, Kampferol and Luteolin, and each 100 g fresh aerial parts will contain 28 mg total flavonoids. The amount of each type of flavonoids were detected by HPLC technique. the extracted flavonoids at concentation of 4mg/ml showed potent effect upon the gram bacteria negative pseudomonas aeruginosa which is known to be more virulence than the gram positive strains but has no effect on Staphylococcus aureus. Also the extracted flavonoids appeared to be affected against the Candida albicans growth. \u0000Conclusion: the ethanolic extractv of locally plant L.draba is effecient to inhibit the growth of pathogenic bacteria and decrease the chances of skin infection that provided the justification for therapeutic potential as supplementary or alternative medicine.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74353071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.588
Ismail Am, TO Al-Khasreji, Maulood Bk
Background:The ferns are well known that they are a rich sources to bioactive compounds mainly flavonoid groups which are very important to protect these plants from environmental stresses. Objective: The object of this work is to determine chemical content (total flavonoids) in methanol extract of Equisetum arvense L. Family Equisetaceae. Methods: mature sporophyte of the fern was collected from Chemi Rezan region in Sulaimaniyah district during May 2016. The methanol extract of the fern was prepared by using Soxhulet apparatus to be used for phytochemical analysis. Resulrs: The outcome of the qualitative analysis for E. arvense extract had showed that the fern was containing active compounds which involve: alkaloids, saponins, tanins and four flavonoids which were identified as: kaempferol, aempferol-O-3- glycoside, leutolin, and querctin in E. arvense by using HPLC technique . In fact the main compound leutolin (100.6 µg/ ml) was recorded quit high concentration. The results were showed that total flavonoids made up almost (179.5 µ/ ml). Conclusion: It was found that total flavonoid have positive correlation with the environment in the studied region.
背景:蕨类植物富含以类黄酮为主的生物活性物质，对保护植物免受环境胁迫具有重要作用。目的:这项工作的目的是确定化学成分(总类黄酮)的甲醇提取木贼属arvense l .家庭木贼科 . 方法:2016年5月在苏莱曼尼亚地区Chemi Rezan地区采集蕨类植物成熟孢子体。采用索氏装置制备甲醇提取物，用于植物化学分析。结果:用高效液相色谱法对香参提取物进行定性分析，发现香参中含有生物碱、皂苷、单宁和4种黄酮类化合物，分别鉴定为山奈酚、山奈酚- o -3-糖苷、羊柳素和槲皮素。事实上，主要化合物leutolin(100.6µg/ ml)被记录为高浓度。结果表明,总类黄酮组成几乎(179.5µ/毫升 ). 结论:研究区黄酮类化合物含量与环境呈正相关。
{"title":"Flavonoids content in methanolic extract of Equisetum arvense L.(Horsetail) from Kurdistan region - Iraq","authors":"Ismail Am, TO Al-Khasreji, Maulood Bk","doi":"10.24126/jobrc.2020.14.1.588","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.588","url":null,"abstract":"Background:The ferns are well known that they are a rich sources to bioactive compounds mainly flavonoid groups which are very important to protect these plants from environmental stresses. Objective: The object of this work is to determine chemical content (total flavonoids) in methanol extract of Equisetum arvense L. Family Equisetaceae. Methods: mature sporophyte of the fern was collected from Chemi Rezan region in Sulaimaniyah district during May 2016. The methanol extract of the fern was prepared by using Soxhulet apparatus to be used for phytochemical analysis. \u0000 Resulrs: The outcome of the qualitative analysis for E. arvense extract had showed that the fern was containing active compounds which involve: alkaloids, saponins, tanins and four flavonoids which were identified as: kaempferol, aempferol-O-3- glycoside, leutolin, and querctin in E. arvense by using HPLC technique . In fact the main compound leutolin (100.6 µg/ ml) was recorded quit high concentration. The results were showed that total flavonoids made up almost (179.5 µ/ ml). \u0000Conclusion: It was found that total flavonoid have positive correlation with the environment in the studied region. \u0000 ","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"382 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79134342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.594
Halah Kh. Al-Samaraie, M. Sabbah, M. Al-Zubaidi
Back ground: It is important to establish STR database for future applications in forensic and paternity tests to gain a reliable evaluation for statistical analysis and that STR database would be more representative. Objective: The objectives of this study are establishing autosomal STR database for the population of Hilla City. Material and methods: DNA was extracted and purified from sixty six individuals from Hilla city by Prepfiller kit. Extracted DNA quantified by Quantifiler real time PCR kit. Fifteen autosomal STR loci (Identifiler) were amplified then analyzed by Genetic Analyzer. All kits supplemented by Applied Biosystem, USA. Results: All samples amplified and analyzed successfully. Allele frequency and forensic parameters were determined. Conclusion: We can conclude that we can use this database for further forensic and paternity applications.
{"title":"Allele Frequencies of 15 Autosomal STR Loci in Hilla City population / Iraq","authors":"Halah Kh. Al-Samaraie, M. Sabbah, M. Al-Zubaidi","doi":"10.24126/jobrc.2020.14.1.594","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.594","url":null,"abstract":"Back ground: It is important to establish STR database for future applications in forensic and paternity tests to gain a reliable evaluation for statistical analysis and that STR database would be more representative. \u0000Objective: The objectives of this study are establishing autosomal STR database for the population of Hilla City. \u0000Material and methods: DNA was extracted and purified from sixty six individuals from Hilla city by Prepfiller kit. Extracted DNA quantified by Quantifiler real time PCR kit. Fifteen autosomal STR loci (Identifiler) were amplified then analyzed by Genetic Analyzer. All kits supplemented by Applied Biosystem, USA. \u0000Results: All samples amplified and analyzed successfully. Allele frequency and forensic parameters were determined. \u0000Conclusion: We can conclude that we can use this database for further forensic and paternity applications.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72736417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.586
H. A. Khaleefah, S. Al-Awadi, Zaid Nsaif Abbas Al-Tameemi
Back ground: X-chromosomal short tandem repeats (X-STRs) have assured to be informative and particular role in complex relationship testing. DXS6807 known as tetra nucleotides polymorphism representing eight alleles of 251-275 bp in length. DXS6807 is located in, at XP 22.2, at a genetic distance of more than 87 and 151 Cm of X-chromosome. DXS101 is located104.9–121 cM from the Xp-telomere (Xp-tel) corresponding to cytogenetic position in Xq21.33–Xq22.3. Objective: The aim of this present study investigates the allele frequency of two markers DXS101, DXS6807 and forensic efficiency parameters for sample of Arabic Iraqi males. Material and methods: The population of this study includes 200 males apparently healthy unrelated participants from different region of Baghdad city, their ages ranged between (20-50) years. The Genomic DNA extracted and purified successfully from blood samples. Results: The forensic efficiency parameters result for these markers were following: polymorphism information content (PIC) of 0.834708, power of discrimination (PD) in male 0.851750, Power of exclusion (PE) 0.698316, MEC Krüger0.511679, MEC Kishida 0.694890. The forensic efficiency parameters analyzing from Arabic population were Power of discrimination (PD) = 0.73405, Polymorphism information content (PIC) =0.69489, Power of exclusion (PE) =0.482879.MEC Krüger =0.511679, MEC Kishida = 0.694890. Conclusions: The information provided establish this X-linked microsatellite marker as a valuable strategy for forensic application. DXS101is and DXS6807 recently consider more stable and suitable forensic markers for forensic application.
{"title":"Genetic analysis of DXS101and DXS6807 short tandem repeat (X-STR) in samples of Iraqi Arab male population","authors":"H. A. Khaleefah, S. Al-Awadi, Zaid Nsaif Abbas Al-Tameemi","doi":"10.24126/jobrc.2020.14.1.586","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.586","url":null,"abstract":"Back ground: X-chromosomal short tandem repeats (X-STRs) have assured to be informative and particular role in complex relationship testing. DXS6807 known as tetra nucleotides polymorphism representing eight alleles of 251-275 bp in length. DXS6807 is located in, at XP 22.2, at a genetic distance of more than 87 and 151 Cm of X-chromosome. DXS101 is located104.9–121 cM from the Xp-telomere (Xp-tel) corresponding to cytogenetic position in Xq21.33–Xq22.3. \u0000Objective: The aim of this present study investigates the allele frequency of two markers DXS101, DXS6807 and forensic efficiency parameters for sample of Arabic Iraqi males. \u0000Material and methods: The population of this study includes 200 males apparently healthy unrelated participants from different region of Baghdad city, their ages ranged between (20-50) years. The Genomic DNA extracted and purified successfully from blood samples. \u0000Results: The forensic efficiency parameters result for these markers were following: polymorphism information content (PIC) of 0.834708, power of discrimination (PD) in male 0.851750, Power of exclusion (PE) 0.698316, MEC Krüger0.511679, MEC Kishida 0.694890. The forensic efficiency parameters analyzing from Arabic population were Power of discrimination (PD) = 0.73405, Polymorphism information content (PIC) =0.69489, Power of exclusion (PE) =0.482879.MEC Krüger =0.511679, MEC Kishida = 0.694890. \u0000Conclusions: The information provided establish this X-linked microsatellite marker as a valuable strategy for forensic application. DXS101is and DXS6807 recently consider more stable and suitable forensic markers for forensic application.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82480797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.592
Ahmed Husham Salman, A. A. Hawezy
Back ground: Helicobacter pylori are bacteria colonize in the human epithelial cells of the gastrointestinal tract. Its infection causes different diseases, including chronic gastritis, peptic ulcers, gastric lymphoma and adenocarcinoma. H. pylori have many virulence factors attributing in one or more biological functions. Objective: Detecting the prevalence of virulence factor genes vacA, cagA, iceA among strain of H. pylori using molecular technique (PCR). Materials and methods: Sixty patients (27 male and 33 female), aged 18 and above included in the present study who showed signs and symptoms of H. pylori, and undergo endoscopy between period of November 2019 and February 2020. RUT and PCR test done to detect the presence of H. pylori infection, also PCR used to detect the three virulence factors. Results: Result showed that 44 patients, 21 (47.7%) male and 23 (52.3%) female were detected as positive H. pylori infections, among them 13 (29.5%) above 50 years, and 31 (70.4%) were below 50 years. While prevalence of the virulence factors vacA, cagA, and iceA were (100%), (84.1%), and (34.1%) respectively. Conclusion: It can be concluded that the frequency and prevalence of these genes are differed and showed significant differences among them. Also, PCR test is sensitive and accurate for detection of H. pylori virulence genes.
{"title":"Prevalence of vacA, cagA, and iceA Virulence Factors of Helicobacter Pylori Isolated from Gastro-duodenal Patients","authors":"Ahmed Husham Salman, A. A. Hawezy","doi":"10.24126/jobrc.2020.14.1.592","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.592","url":null,"abstract":"Back ground: Helicobacter pylori are bacteria colonize in the human epithelial cells of the gastrointestinal tract. Its infection causes different diseases, including chronic gastritis, peptic ulcers, gastric lymphoma and adenocarcinoma. H. pylori have many virulence factors attributing in one or more biological functions. \u0000Objective: Detecting the prevalence of virulence factor genes vacA, cagA, iceA among strain of H. pylori using molecular technique (PCR). \u0000Materials and methods: Sixty patients (27 male and 33 female), aged 18 and above included in the present study who showed signs and symptoms of H. pylori, and undergo endoscopy between period of November 2019 and February 2020. RUT and PCR test done to detect the presence of H. pylori infection, also PCR used to detect the three virulence factors. \u0000Results: Result showed that 44 patients, 21 (47.7%) male and 23 (52.3%) female were detected as positive H. pylori infections, among them 13 (29.5%) above 50 years, and 31 (70.4%) were below 50 years. While prevalence of the virulence factors vacA, cagA, and iceA were (100%), (84.1%), and (34.1%) respectively. \u0000Conclusion: It can be concluded that the frequency and prevalence of these genes are differed and showed significant differences among them. Also, PCR test is sensitive and accurate for detection of H. pylori virulence genes.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86530549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.587
N. Tahir, H. Ahmed, R. Hashim, Teba D. Soluiman
Background: Obesity and type 2 diabetes have both rapidly raised during the last periods and are ongoing to increase at a disturbing rate universal. Several clinical and epidemiological researches demonstrated a reverse association between circulating vitamin D levels, central adiposity and the progress of insulin resistance and diabetes. Objective: The target of this work was to elucidate the complex role of vitamin D and the clinical implications of diabetes on metabolic defects related with obesity. Subjects and Methods: This study encompassed 90 diabetic patients (45 obese and 45 non obese) who were attending the National Diabetic Center/ Al-Mustansiriyah University during the period from June 2019 to January 2020; their age range was (35-60) years. All participant underwent clinical and biochemical examinations. Results: A substantial rise (p= 0.01) in waist/hip ratio, body mass index, fasting serum glucose, total cholesterol, triacylglycerol, and low density lipoprotein cholesterol in obese diabetic patients as paralleled to non-obese group. Moreover, there was an elevation in glycated hemoglobin, serum insulin, and homeostasis model assessment for insulin resistance in obese group, but it was not significant. A substantial decrease (p= 0.01) in serum high density lipoprotein cholesterol and vitamin D3 were detected in obese diabetic patients as paralleled to non-obese group. Also, obese diabetic patients had the higher percent (61%) of D3 deficiency as paralleled to non-obese patients. Conclusions: In the present study, it is found that there is significant increase in blood sugar in the individuals with decreased vitamin D levels, which was related with insulin resistance, decreased β-cell function, and obesity.
{"title":"The Correlation between Vitamin D and Clinical Implications for Obesity-Related Type 2 Diabetes Mellitus","authors":"N. Tahir, H. Ahmed, R. Hashim, Teba D. Soluiman","doi":"10.24126/jobrc.2020.14.1.587","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.587","url":null,"abstract":"Background: Obesity and type 2 diabetes have both rapidly raised during the last periods and are ongoing to increase at a disturbing rate universal. Several clinical and epidemiological researches demonstrated a reverse association between circulating vitamin D levels, central adiposity and the progress of insulin resistance and diabetes. \u0000Objective: The target of this work was to elucidate the complex role of vitamin D and the clinical implications of diabetes on metabolic defects related with obesity. \u0000Subjects and Methods: This study encompassed 90 diabetic patients (45 obese and 45 non obese) who were attending the National Diabetic Center/ Al-Mustansiriyah University during the period from June 2019 to January 2020; their age range was (35-60) years. All participant underwent clinical and biochemical examinations. \u0000Results: A substantial rise (p= 0.01) in waist/hip ratio, body mass index, fasting serum glucose, total cholesterol, triacylglycerol, and low density lipoprotein cholesterol in obese diabetic patients as paralleled to non-obese group. Moreover, there was an elevation in glycated hemoglobin, serum insulin, and homeostasis model assessment for insulin resistance in obese group, but it was not significant. A substantial decrease (p= 0.01) in serum high density lipoprotein cholesterol and vitamin D3 were detected in obese diabetic patients as paralleled to non-obese group. \u0000Also, obese diabetic patients had the higher percent (61%) of D3 deficiency as paralleled to non-obese patients. \u0000Conclusions: In the present study, it is found that there is significant increase in blood sugar in the individuals with decreased vitamin D levels, which was related with insulin resistance, decreased β-cell function, and obesity. \u0000 ","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88787591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.593
A. S. Atiyah, Marwa H. Alkhafaji
Background: The microbial production of substances that have the potency to suppress the growth of other microorganisms is probably one of the prevalent defense strategy developed in nature, microorganisms produce a variable bunch of microbial defense systems, which include antibiotics, metabolic by-products, lytic agents, bacteriocins and others. Objective: The purpose of the present study was to isolate and identify Enterococcus faecium isolates then detecting its ability of carrying the gene responsible for enterocin production in this species. Materials and methods: Out of 50 samples from different sources (food and clinical sources) were collected for the Enterococcus faecium isolation, and the isolated bacteria Enterococcus faecium (37) isolates were detected for their harboring of Enterocin A gene (entA), using conventional PCR technique. Results: The identification revealed that 37(74%) isolates were considered as Enterococcus faecium, 20 isolates (54.05%) out of food samples (10 samples were collected from dairies, 7 from vegetables and 3 from fish samples), and 17 isolates 45.9% out of clinical samples (11 from stool and 6 from urine source). Genotypic Detection done by the amplification of the enterocin coding gene (ent A), and the results revealed that all the isolates were harboring that gene despite of the phonotypical differences, that they amplified entA gene and the PCR product size (362 bp) was detected using agarose gel electrophoresis. Conclusions: This study indicates the presence of Enterococcus spp. in food and clinical sources and the ability of these bacteria to produce antibacterial substances which is active against closely related clinical isolates.
{"title":"Molecular screening of the entA gene of Enterococcus faecium isolated from Food and clinical sources","authors":"A. S. Atiyah, Marwa H. Alkhafaji","doi":"10.24126/jobrc.2020.14.1.593","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.593","url":null,"abstract":"Background: The microbial production of substances that have the potency to suppress the growth of other microorganisms is probably one of the prevalent defense strategy developed in nature, microorganisms produce a variable bunch of microbial defense systems, which include antibiotics, metabolic by-products, lytic agents, bacteriocins and others. \u0000Objective: The purpose of the present study was to isolate and identify Enterococcus faecium isolates then detecting its ability of carrying the gene responsible for enterocin production in this species. Materials and methods: Out of 50 samples from different sources (food and clinical sources) were collected for the Enterococcus faecium isolation, and the isolated bacteria Enterococcus faecium (37) isolates were detected for their harboring of Enterocin A gene (entA), using conventional PCR technique. \u0000Results: The identification revealed that 37(74%) isolates were considered as Enterococcus faecium, 20 isolates (54.05%) out of food samples (10 samples were collected from dairies, 7 from vegetables and 3 from fish samples), and 17 isolates 45.9% out of clinical samples (11 from stool and 6 from urine source). Genotypic Detection done by the amplification of the enterocin coding gene (ent A), and the results revealed that all the isolates were harboring that gene despite of the phonotypical differences, that they amplified entA gene and the PCR product size (362 bp) was detected using agarose gel electrophoresis. \u0000Conclusions: This study indicates the presence of Enterococcus spp. in food and clinical sources and the ability of these bacteria to produce antibacterial substances which is active against closely related clinical isolates.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77113993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24126/jobrc.2020.14.1.595
Abdullateef M. AL Beatushi, Imad Hatem Hussein, Raghad S. Mouhamad, K. A. al-Khafaji, Mohammed W. Tayh, Razaq W. Al-Zamily, Haidar A. Ghlaim, Qusay kh. Abbas
Background:The most important medical challenge is the emergence of bacterial resistant to traditional antibiotics. So the need for new pharmaceutical compound derived from daily intake plant material. Mint and black tea used extensively in Arabic area as hot or cold drink Objective: investigate the bacterial activity of mint and black tea aqueous extract against many bacterial genera. Material and methods: from the local market of Baghdad Province, Iraq, dry black tea was obtained; while, peppermint (Mentha piperita L) was harvested from Iraqi plants during April 2020. Active ingredients extracted using either ethanol or water and their antibacterial activity evaluated against Staphylococcus aureus, S.epidermidis, Citrobacter sp and Klebsiella sp using well diffusion agar under invitro laboratory condition. Results: Dried powder of each extract appeared with different color range from brown to black. Results recognized that S. aureus was susceptible to watery and ethanolic extract of both black tea and peppermint extracts. While, S. epidermidis showed resist to both plant extract and solvents used in extraction except minor inhibition with ethanolic extract of tea at 0.3% dose dose. Citrobacter spp gave susceptibility to watery extract of black tea as well as, watery and ethanolic of mint extract. Otherwise, Klebsiella spp. Growth inhibited by watery extract of black tea while, no bioactivity existed upon mint extract treatment. Conclusion: Dose dependent manner existed for watery and ethanolic extracts for both plant material in their antibacterial activity.
{"title":"Antibacterial activity of watery and ethanolic extracts of Black Tea and peppermint (In vitrostudy)","authors":"Abdullateef M. AL Beatushi, Imad Hatem Hussein, Raghad S. Mouhamad, K. A. al-Khafaji, Mohammed W. Tayh, Razaq W. Al-Zamily, Haidar A. Ghlaim, Qusay kh. Abbas","doi":"10.24126/jobrc.2020.14.1.595","DOIUrl":"https://doi.org/10.24126/jobrc.2020.14.1.595","url":null,"abstract":"Background:The most important medical challenge is the emergence of bacterial resistant to traditional antibiotics. So the need for new pharmaceutical compound derived from daily intake plant material. Mint and black tea used extensively in Arabic area as hot or cold drink \u0000Objective: investigate the bacterial activity of mint and black tea aqueous extract against many bacterial genera. \u0000Material and methods: from the local market of Baghdad Province, Iraq, dry black tea was obtained; while, peppermint (Mentha piperita L) was harvested from Iraqi plants during April 2020. Active ingredients extracted using either ethanol or water and their antibacterial activity evaluated against Staphylococcus aureus, S.epidermidis, Citrobacter sp and Klebsiella sp using well diffusion agar under invitro laboratory condition. \u0000 Results: Dried powder of each extract appeared with different color range from brown to black. Results recognized that S. aureus was susceptible to watery and ethanolic extract of both black tea and peppermint extracts. While, S. epidermidis showed resist to both plant extract and solvents used in extraction except minor inhibition with ethanolic extract of tea at 0.3% dose dose. Citrobacter spp gave susceptibility to watery extract of black tea as well as, watery and ethanolic of mint extract. Otherwise, Klebsiella spp. Growth inhibited by watery extract of black tea while, no bioactivity existed upon mint extract treatment. \u0000Conclusion: Dose dependent manner existed for watery and ethanolic extracts for both plant material in their antibacterial activity. \u0000 ","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87516741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-02DOI: 10.32861/jbr.512.128.139
Tailise Beatriz Roll Zimmer, D. Otero, R. Zambiazi
Physalis pubescens L. has exotic fruit, native to the Americas, with potential to be widely used for consumption. This plant has yellow fruits accompanied by chalices. The aerial parts are used in traditional medicine due to its preventive and curative effect. Its fruits are aromatic and mildly acidic, besides containing compounds that contribute to the functional capacity of the fruit. Due to the health benefits, the use of this plant has generated a great commercial interest. Purpose of this review was to compile information on the functional properties of Physalis pubescens, as well as to explore information on some of the major species of Physalis.
{"title":"Functional Properties of Physalis Pubescens Linnaeus: A Literature Review","authors":"Tailise Beatriz Roll Zimmer, D. Otero, R. Zambiazi","doi":"10.32861/jbr.512.128.139","DOIUrl":"https://doi.org/10.32861/jbr.512.128.139","url":null,"abstract":"Physalis pubescens L. has exotic fruit, native to the Americas, with potential to be widely used for consumption. This plant has yellow fruits accompanied by chalices. The aerial parts are used in traditional medicine due to its preventive and curative effect. Its fruits are aromatic and mildly acidic, besides containing compounds that contribute to the functional capacity of the fruit. Due to the health benefits, the use of this plant has generated a great commercial interest. Purpose of this review was to compile information on the functional properties of Physalis pubescens, as well as to explore information on some of the major species of Physalis.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87135223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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