Pub Date : 2026-03-01Epub Date: 2026-03-12DOI: 10.1016/j.jaim.2025.101242
LiQi Li , YuHong Zheng , JiaXin Li , Lin Zhao , Xin Lin , Hao Zhang , HuiHui Chen , PeiYu Yan
<div><h3>Background</h3><div>The incidence of pulmonary diseases remains unacceptably high, underscoring the need for improved therapeutic strategies. The current therapeutic landscape, largely reliant on antibiotics and hormonal agents, is constrained by growing challenges such as drug resistance, significant toxicity profiles, and prolonged disease courses. As a time-honored TCM prescription, Weijing Decoction (WJD) is widely administered for respiratory ailments. However, a critical appraisal of the existing literature reveals a paucity of high-quality evidence. Research to date has largely been confined to anecdotal case reports or preliminary investigations with limited sample sizes, resulting in a lack of systematic assessment on the combined efficacy and safety of WJD and modern medical interventions.</div></div><div><h3>Objective</h3><div>This study aimed to systematically evaluate and meta-analyze the therapeutic efficacy of Weijing Decoction (WJD) in the management of respiratory diseases, including chronic obstructive pulmonary disease (COPD) and pneumonia.</div></div><div><h3>Methods</h3><div>A comprehensive literature search was conducted across six electronic databases, including both international and Chinese-language databases, to identify randomized controlled trials (RCTs) evaluating the efficacy of WJD in the treatment of COPD and pneumonia. Data on primary outcomes, including the overall clinical efficacy rate, C-reactive protein (CRP) levels, and Traditional Chinese Medicine Syndrome Scores, were extracted and synthesized using RevMan version 5.4 software. Statistical heterogeneity across studies was assessed using the I<sup>2</sup> test and Chi-square test. A fixed-effect model was employed in the presence of low heterogeneity (I<sup>2</sup> ≤ 50 %, p > 0.1), whereas a random-effects model was applied when significant heterogeneity was observed (I<sup>2</sup> > 50 %, p < 0.1). Funnel plots were used to assess potential publication bias among the included studies.</div></div><div><h3>Results</h3><div>A total of 10 eligible studies involving 1315 patients diagnosed with respiratory diseases were included in the analysis. Patients in the control group received conventional medical treatment, whereas those in the intervention group received WJD in addition to standard therapy. The intervention group demonstrated significantly higher overall disease efficacy rates, along with marked reductions in CRP levels and lower Traditional Chinese Medicine Syndrome Scores (including symptoms such as cough and sputum production), compared to the control group (all p < 0.05).</div></div><div><h3>Conclusion</h3><div>The combination of WJD with conventional therapy appears to be an effective and beneficial treatment strategy for managing COPD and pneumonia, potentially improving clinical outcomes. This integrative approach shows promise in enhancing the quality of care and may offer additional therapeutic advantages in the managemen
{"title":"The classic Chinese medicine weijing decoction as a complementary intervention for respiratory diseases: A systematic review and meta-analysis","authors":"LiQi Li , YuHong Zheng , JiaXin Li , Lin Zhao , Xin Lin , Hao Zhang , HuiHui Chen , PeiYu Yan","doi":"10.1016/j.jaim.2025.101242","DOIUrl":"10.1016/j.jaim.2025.101242","url":null,"abstract":"<div><h3>Background</h3><div>The incidence of pulmonary diseases remains unacceptably high, underscoring the need for improved therapeutic strategies. The current therapeutic landscape, largely reliant on antibiotics and hormonal agents, is constrained by growing challenges such as drug resistance, significant toxicity profiles, and prolonged disease courses. As a time-honored TCM prescription, Weijing Decoction (WJD) is widely administered for respiratory ailments. However, a critical appraisal of the existing literature reveals a paucity of high-quality evidence. Research to date has largely been confined to anecdotal case reports or preliminary investigations with limited sample sizes, resulting in a lack of systematic assessment on the combined efficacy and safety of WJD and modern medical interventions.</div></div><div><h3>Objective</h3><div>This study aimed to systematically evaluate and meta-analyze the therapeutic efficacy of Weijing Decoction (WJD) in the management of respiratory diseases, including chronic obstructive pulmonary disease (COPD) and pneumonia.</div></div><div><h3>Methods</h3><div>A comprehensive literature search was conducted across six electronic databases, including both international and Chinese-language databases, to identify randomized controlled trials (RCTs) evaluating the efficacy of WJD in the treatment of COPD and pneumonia. Data on primary outcomes, including the overall clinical efficacy rate, C-reactive protein (CRP) levels, and Traditional Chinese Medicine Syndrome Scores, were extracted and synthesized using RevMan version 5.4 software. Statistical heterogeneity across studies was assessed using the I<sup>2</sup> test and Chi-square test. A fixed-effect model was employed in the presence of low heterogeneity (I<sup>2</sup> ≤ 50 %, p > 0.1), whereas a random-effects model was applied when significant heterogeneity was observed (I<sup>2</sup> > 50 %, p < 0.1). Funnel plots were used to assess potential publication bias among the included studies.</div></div><div><h3>Results</h3><div>A total of 10 eligible studies involving 1315 patients diagnosed with respiratory diseases were included in the analysis. Patients in the control group received conventional medical treatment, whereas those in the intervention group received WJD in addition to standard therapy. The intervention group demonstrated significantly higher overall disease efficacy rates, along with marked reductions in CRP levels and lower Traditional Chinese Medicine Syndrome Scores (including symptoms such as cough and sputum production), compared to the control group (all p < 0.05).</div></div><div><h3>Conclusion</h3><div>The combination of WJD with conventional therapy appears to be an effective and beneficial treatment strategy for managing COPD and pneumonia, potentially improving clinical outcomes. This integrative approach shows promise in enhancing the quality of care and may offer additional therapeutic advantages in the managemen","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 2","pages":"Article 101242"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147450633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-03-11DOI: 10.1016/j.jaim.2025.101313
Mansoor Ali , Reenu Punia , Navneendra Singh , Yasmeen Shamsi , Rana P. Singh
Background
Lung cancer is among the most common and lethal types of cancer in the world. The Unani system of medicine is an ancient traditional medicine that utilizes various medicinal plants.
Objective
This study aimed to investigate a herbal formulation, Safoof Lajward (SL) which has not been studied for its chemopreventive and anticancer properties to date.
Materials and Methods
Methanolic extract of SL was chemically characterised by GC-MS and HPLC and its in vivo toxicity was evaluated on C57BL/6 mice. Anticancer effects of SL were studied on human lung carcinoma cells using trypan blue stain, clonogenicity, cell cycle distribution, apoptosis, mitochondrial membrane potential (ΔΨm), and immunoblotting assays. The antitumor effect of SL was evaluated on A549 lung tumor xenograft in nude mice.
Results
SL showed various bioactive phytochemicals like resveratrol, epicatechin, ellagic acid, epicatechin gallate, quercetin, and vanillin. The orally administered SL (50 and 100 mg/kg) elevated the hepatic xenobiotic-metabolizing enzymes (phases I & II). SL inhibited the growth and survival of lung cancer cells by accumulating cells at the sub-G1 phase and increasing apoptotic cells by 47-fold and caused dissipation of ΔΨm by 7-fold with increased expression of Bax and decreased expression of Bcl-2 proteins. SL induced DNA damage by reducing Chk1, Chk2, and Rad51 proteins. SL strongly suppressed A549 lung tumor xenograft growth by 45 % and tumor weight by 53 % without any toxicity in mice. SL inhibited Ki-67 and PCNA expression and enhanced Bax/Bcl-2 ratio, cleaved PARP, and cleaved caspase 3, but decreased Chk1, Chk2, and Rad51 proteins in tumors.
Conclusions
Together, these findings suggested that SL has chemopreventive efficacy as well as strong anti-proliferative and pro-apoptotic activities against lung cancer.
{"title":"A herbal formulation inhibits growth and survival of lung cancer cells through DNA damage and apoptosis - in vitro and in vivo studies","authors":"Mansoor Ali , Reenu Punia , Navneendra Singh , Yasmeen Shamsi , Rana P. Singh","doi":"10.1016/j.jaim.2025.101313","DOIUrl":"10.1016/j.jaim.2025.101313","url":null,"abstract":"<div><h3>Background</h3><div>Lung cancer is among the most common and lethal types of cancer in the world. The Unani system of medicine is an ancient traditional medicine that utilizes various medicinal plants.</div></div><div><h3>Objective</h3><div>This study aimed to investigate a herbal formulation, <em>Safoof Lajward</em> (SL) which has not been studied for its chemopreventive and anticancer properties to date.</div></div><div><h3>Materials and Methods</h3><div>Methanolic extract of SL was chemically characterised by GC-MS and HPLC and its <em>in vivo</em> toxicity was evaluated on C57BL/6 mice. Anticancer effects of SL were studied on human lung carcinoma cells using trypan blue stain, clonogenicity, cell cycle distribution, apoptosis, mitochondrial membrane potential (ΔΨm), and immunoblotting assays. The antitumor effect of SL was evaluated on A549 lung tumor xenograft in nude mice.</div></div><div><h3>Results</h3><div>SL showed various bioactive phytochemicals like resveratrol, epicatechin, ellagic acid, epicatechin gallate, quercetin, and vanillin. The orally administered SL (50 and 100 mg/kg) elevated the hepatic xenobiotic-metabolizing enzymes (phases I & II). SL inhibited the growth and survival of lung cancer cells by accumulating cells at the sub-G1 phase and increasing apoptotic cells by 47-fold and caused dissipation of ΔΨm by 7-fold with increased expression of Bax and decreased expression of Bcl-2 proteins. SL induced DNA damage by reducing Chk1, Chk2, and Rad51 proteins. SL strongly suppressed A549 lung tumor xenograft growth by 45 % and tumor weight by 53 % without any toxicity in mice. SL inhibited Ki-67 and PCNA expression and enhanced Bax/Bcl-2 ratio, cleaved PARP, and cleaved caspase 3, but decreased Chk1, Chk2, and Rad51 proteins in tumors.</div></div><div><h3>Conclusions</h3><div>Together, these findings suggested that SL has chemopreventive efficacy as well as strong anti-proliferative and pro-apoptotic activities against lung cancer.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 2","pages":"Article 101313"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147427461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-01Epub Date: 2026-02-25DOI: 10.1016/j.jaim.2026.101323
Maitri J. Parmar , Rutu D. Shah , Kalapi B. Patel , Shivenarain N. Gupta , Manish V. Patel
Snake bite complication often lead to tissue necrosis, cellulitis, non-healing ulcers and systemic disturbance such as coagulopathy and ascites, making management highly challenging. A 50-year-old male presented with chronic non-healing ulcer on the right limb that persisted for 58 days despite conventional treatment and was associated with severe pain, foul odour and deep tissue involvement including subcutaneous tissue and facia, bone and muscle were not exposed. The ulcer measured 11.5 cm in length, 6.2 cm in width and 5 mm in depth with necrotic fascia involvement. The patient also had decompensated liver cirrhosis, characteristic by ascites (abdominal girth 95 cm), pitting oedema, oliguria, urinary dribbling, low serum albumin (3.4 g/dL) and elevated total bilirubin (2.7 mg/dL). Therefore, the overall prognosis remains poor owing to advanced hepatic decompensation with associated hepatorenal involvement and necrotic fascio-muscular changes, indicating multi-organ dysfunction and markedly reduced physiological reserve. Ayurveda management was initiated with vardhamana pippali followed by virechana, along with supportive therapies including vrana prakshalana, vrana ropana and jalaukavacharana. Management based on vrana and yakritodara chikitsa improved ulcer healing, liver function and ascites, leading to a better clinical outcome despite a poor prognosis. Over two months, there was progressive ulcer healing with a reduction in ulcer size to 4.5 cm in length, 2 cm in width and 2mm in depth, complete resolution of oedema, 29 cm decrease in abdominal girth, improved liver function (serum albumin – 4.1 g/dL, total bilirubin 0.8 mg/dL) and relief in urinary complaints. Subsequently, complete healing was achieved after 10 months, with no recurrence of the ulcer and ascites observed during the follow-up period. The Child-Pugh score improved from Grade C to Grade A. This case highlights the potential role of Ayurveda interventions in chronic non-healing ulcers with systemic complications.
{"title":"Management of a chronic non-healing viper bite ulcer in a patient with alcoholic liver cirrhosis: A Case Report","authors":"Maitri J. Parmar , Rutu D. Shah , Kalapi B. Patel , Shivenarain N. Gupta , Manish V. Patel","doi":"10.1016/j.jaim.2026.101323","DOIUrl":"10.1016/j.jaim.2026.101323","url":null,"abstract":"<div><div>Snake bite complication often lead to tissue necrosis, cellulitis, non-healing ulcers and systemic disturbance such as coagulopathy and ascites, making management highly challenging. A 50-year-old male presented with chronic non-healing ulcer on the right limb that persisted for 58 days despite conventional treatment and was associated with severe pain, foul odour and deep tissue involvement including subcutaneous tissue and facia, bone and muscle were not exposed. The ulcer measured 11.5 cm in length, 6.2 cm in width and 5 mm in depth with necrotic fascia involvement. The patient also had decompensated liver cirrhosis, characteristic by ascites (abdominal girth 95 cm), pitting oedema, oliguria, urinary dribbling, low serum albumin (3.4 g/dL) and elevated total bilirubin (2.7 mg/dL). Therefore, the overall prognosis remains poor owing to advanced hepatic decompensation with associated hepatorenal involvement and necrotic fascio-muscular changes, indicating multi-organ dysfunction and markedly reduced physiological reserve. Ayurveda management was initiated with <em>vardhamana pippali</em> followed by <em>virechana</em>, along with supportive therapies including <em>vrana prakshalana, vrana ropana</em> and <em>jalaukavacharana</em>. Management based on <em>vrana</em> and <em>yakritodara chikitsa</em> improved ulcer healing, liver function and ascites, leading to a better clinical outcome despite a poor prognosis. Over two months, there was progressive ulcer healing with a reduction in ulcer size to 4.5 cm in length, 2 cm in width and 2mm in depth, complete resolution of oedema, 29 cm decrease in abdominal girth, improved liver function (serum albumin – 4.1 g/dL, total bilirubin 0.8 mg/dL) and relief in urinary complaints. Subsequently, complete healing was achieved after 10 months, with no recurrence of the ulcer and ascites observed during the follow-up period. The Child-Pugh score improved from Grade C to Grade A. This case highlights the potential role of Ayurveda interventions in chronic non-healing ulcers with systemic complications.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 2","pages":"Article 101323"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147306198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoga is recognized as a holistic practice promoting multidimensional well-being. However, the influence of practice timing in alignment with circadian rhythms remains underexplored despite its potential to optimize therapeutic outcomes.
Objective
To assess the differential effects of morning versus evening tele-yoga on quality of life, sleep, psychological health and lifestyle behaviours among young adults.
Materials and methods
A randomized controlled trial with a three-arm, pre-post design was conducted at a business school in Bengaluru from May 2022 to March 2023. A total of 156 postgraduate students were randomized into morning yoga (6:00 am to 7:00 am), evening yoga (6:00 pm to 7:00 pm) and wait-list control groups. Eighty-two participants (42 males, 40 females; mean age = 22.54 ± 1.67 years) completed a four-week tele-yoga intervention (1 h/day, 5 days/week). Assessments included WHOQOL-BREF, PSQI, DASS-21, Vedic Personality Inventory, Morningness-Eveningness Questionnaire and lifestyle behaviour measures.
Linear mixed-effects models with Bonferroni correction were used to analyse repeated measures and missing data.
Results
Morning yoga group showed significant benefits over evening yoga as well as control in reducing sleep disturbances (p = 0.03), enhancing morningness (p = 0.004), increasing sattva (positive mental health) traits (p = 0.02) and reducing junk food intake (p = 0.03). Both yoga groups outperformed controls in improving quality of life, psychological health, sleep and lifestyle measures (p < 0.001–0.031). As compared to the controls, morning yoga group uniquely improved energy (p = 0.007) and restfulness (p = 0.006), while evening yoga reduced sadness (p = 0.021). Lifestyle benefits emerged without explicit behavioral advice.
Conclusion
Morning yoga yields superior benefits for sleep, chronotype, sattva traits, and dietary choices. Aligning yoga practice with circadian rhythms may optimize health outcomes in young adults.
{"title":"Dawn vs. Dusk: Yoga practice timing shapes sleep, mood and well-being in young adults - results of a randomized controlled trial","authors":"Swathi Iyer , Raghavendra Bhat , Kankan Gulati , Hemant Bhargav","doi":"10.1016/j.jaim.2025.101314","DOIUrl":"10.1016/j.jaim.2025.101314","url":null,"abstract":"<div><h3>Background</h3><div>Yoga is recognized as a holistic practice promoting multidimensional well-being. However, the influence of practice timing in alignment with circadian rhythms remains underexplored despite its potential to optimize therapeutic outcomes.</div></div><div><h3>Objective</h3><div>To assess the differential effects of morning versus evening tele-yoga on quality of life, sleep, psychological health and lifestyle behaviours among young adults.</div></div><div><h3>Materials and methods</h3><div>A randomized controlled trial with a three-arm, pre-post design was conducted at a business school in Bengaluru from May 2022 to March 2023. A total of 156 postgraduate students were randomized into morning yoga (6:00 am to 7:00 am), evening yoga (6:00 pm to 7:00 pm) and wait-list control groups. Eighty-two participants (42 males, 40 females; mean age = 22.54 ± 1.67 years) completed a four-week tele-yoga intervention (1 h/day, 5 days/week). Assessments included WHOQOL-BREF, PSQI, DASS-21, Vedic Personality Inventory, Morningness-Eveningness Questionnaire and lifestyle behaviour measures.</div><div>Linear mixed-effects models with Bonferroni correction were used to analyse repeated measures and missing data.</div></div><div><h3>Results</h3><div>Morning yoga group showed significant benefits over evening yoga as well as control in reducing sleep disturbances (p = 0.03), enhancing morningness (p = 0.004), increasing s<em>attva</em> (positive mental health) traits (p = 0.02) and reducing junk food intake (p = 0.03). Both yoga groups outperformed controls in improving quality of life, psychological health, sleep and lifestyle measures (p < 0.001–0.031). As compared to the controls, morning yoga group uniquely improved energy (p = 0.007) and restfulness (p = 0.006), while evening yoga reduced sadness (p = 0.021). Lifestyle benefits emerged without explicit behavioral advice.</div></div><div><h3>Conclusion</h3><div>Morning yoga yields superior benefits for sleep, chronotype, <em>sattva</em> traits, and dietary choices. Aligning yoga practice with circadian rhythms may optimize health outcomes in young adults.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 2","pages":"Article 101314"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147450634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarcopenia is a degenerative musculoskeletal condition that affects older persons with the hallmark of loss of muscle mass and function. Modern management is limited to nutrition, exercise, and lifestyle modifications, with no approved drugs. Ayurveda recognizes a similar condition called Mamsa kshaya due to vitiation of Vata dosha, which reflects the clinical picture of sarcopenia, and offers Ayush Ahara, a therapeutic dietary approach.
To assess the clinical evidence for the role of traditional Indian diet (∼Ayush ahara) in improving muscle strength and managing sarcopenia.
A scoping review was conducted according to PRISMA-ScR. Literature published from 2015 to 2025 was searched across various databases. Inclusion criteria: randomized controlled trials involving human subjects assessing the effect of traditional Indian diet on muscle strength-related outcomes. Non-English, non-full-text, in vitro/in vivo studies, and reviews were excluded.
The nine studies were included. Ayush ahara, such as green gram, dried grapes, turmeric, cow milk, finger millet, spinach, and almonds, was associated with improved muscle strength, physical performance, reduced inflammation, and enhanced bone mineral density.
Ayush ahara shows potential for improving muscle strength and managing sarcopenia. The reviewed RCTs showed statistical improvements in muscle strength measures, indicating the need for targeted clinical trials to validate these preliminary findings. Support further exploration of Ayurveda-based nutritional strategies as a complementary approach to musculoskeletal aging.
{"title":"Efficacy of traditional Indian diet (Ayush ahara) on muscle strength and Sarcopenia: A scoping review","authors":"Rahul Katkar , Usha Rana , Sriloy Mohanty , Monika Pathania","doi":"10.1016/j.jaim.2025.101265","DOIUrl":"10.1016/j.jaim.2025.101265","url":null,"abstract":"<div><div>Sarcopenia is a degenerative musculoskeletal condition that affects older persons with the hallmark of loss of muscle mass and function. Modern management is limited to nutrition, exercise, and lifestyle modifications, with no approved drugs. Ayurveda recognizes a similar condition called <em>Mamsa kshaya</em> due to vitiation of <em>Vata dosha</em>, which reflects the clinical picture of sarcopenia, and offers <em>Ayush Ahara</em>, a therapeutic dietary approach.</div><div>To assess the clinical evidence for the role of traditional Indian diet (∼<em>Ayush ahara</em>) in improving muscle strength and managing sarcopenia.</div><div>A scoping review was conducted according to PRISMA-ScR. Literature published from 2015 to 2025 was searched across various databases. Inclusion criteria: randomized controlled trials involving human subjects assessing the effect of traditional Indian diet on muscle strength-related outcomes. Non-English, non-full-text, in vitro/in vivo studies, and reviews were excluded.</div><div>The nine studies were included. <em>Ayush ahara,</em> such as green gram, dried grapes, turmeric, cow milk, finger millet, spinach, and almonds, was associated with improved muscle strength, physical performance, reduced inflammation, and enhanced bone mineral density.</div><div><em>Ayush ahara</em> shows potential for improving muscle strength and managing sarcopenia. The reviewed RCTs showed statistical improvements in muscle strength measures, indicating the need for targeted clinical trials to validate these preliminary findings. Support further exploration of Ayurveda-based nutritional strategies as a complementary approach to musculoskeletal aging.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 1","pages":"Article 101265"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01Epub Date: 2026-02-06DOI: 10.1016/j.jaim.2025.101297
Suchisnigdha Datta , Anupam Bishayee , Dona Sinha
Background
Lung cancer therapy resistance is often associated with the redox-regulatory nuclear factor erythroid 2-related factor-2 (NRF2)-Kelch-like ECH-associated protein1 (KEAP1) dysfunction.
Objective
This study investigated the impact of commercially available black tea (BT) phytochemicals (≥80 % theaflavins) from Camellia sinensis in sensitizing doxorubicin (Dox) against nonresponsive lung adenocarcinoma cells by modulation of non-canonical NRF2 regulators.
Methods
The methods included multidrug resistance (MDR) assay, comet assay, cell cycle analysis, zymography, semi-quantitative polymerase chain reaction, western blot and immunocytochemistry (ICC).
Results
BT pretreatment followed by Dox exposure was partially effective in Dox resistance-reversal in A549 cells by increasing drug uptake and downregulating MDR pumps. This combination induced DNA damage and cytotoxicity in A549 cells. It also reduced invasiveness and suppressed the expression of multidrug resistance protein-1, epidermal growth factor receptor, protein kinase B, and B cell lymphoma-2. In absence of wild-type KEAP1, non-KEAP1 regulators were thoroughly investigated by immunolocalization, and immunoblotting. BT restricted non-canonical NRF2 activators, such as p21 and apurinic/apyrimidinic endonuclease1 in A549 and acted oppositely in NCI–H23 cells. Additionally, NRF2-repressors, namely forkhead box O3, p53, glycogen synthase kinase-3β, and retinoid X receptor (RXR), were downregulated in NCI–H23 and upregulated in A549 cells. ICC exhibited that BT modulated the co-localization of NRF2 regulators, such as β-transducin repeat-containing protein and RXR, in A549 and NCI–H23 cells.
Conclusion
Therefore, it might be indicated that BT improved Dox retention and increased the Dox responsiveness in A549 cells. BT-mediated selective suppression of the NRF2, re-stabilized the KEAP-1-independent NRF2 regulators and made the non-responsive A549 cells partially sensitive to Dox.
{"title":"Bioactive black tea phytochemicals partially influenced doxorubicin sensitivity by modulation of NRF2-regulatory pathways in lung cancer","authors":"Suchisnigdha Datta , Anupam Bishayee , Dona Sinha","doi":"10.1016/j.jaim.2025.101297","DOIUrl":"10.1016/j.jaim.2025.101297","url":null,"abstract":"<div><h3>Background</h3><div>Lung cancer therapy resistance is often associated with the redox-regulatory nuclear factor erythroid 2-related factor-2 (NRF2)-Kelch-like ECH-associated protein1 (KEAP1) dysfunction.</div></div><div><h3>Objective</h3><div>This study investigated the impact of commercially available black tea (BT) phytochemicals (≥80 % theaflavins) from <em>Camellia sinensis</em> in sensitizing doxorubicin (Dox) against nonresponsive lung adenocarcinoma cells by modulation of non-canonical NRF2 regulators.</div></div><div><h3>Methods</h3><div>The methods included multidrug resistance (MDR) assay, comet assay, cell cycle analysis, zymography, semi-quantitative polymerase chain reaction, western blot and immunocytochemistry (ICC).</div></div><div><h3>Results</h3><div>BT pretreatment followed by Dox exposure was partially effective in Dox resistance-reversal in A549 cells by increasing drug uptake and downregulating MDR pumps. This combination induced DNA damage and cytotoxicity in A549 cells. It also reduced invasiveness and suppressed the expression of multidrug resistance protein-1, epidermal growth factor receptor, protein kinase B, and B cell lymphoma-2. In absence of wild-type KEAP1, non-KEAP1 regulators were thoroughly investigated by immunolocalization, and immunoblotting. BT restricted non-canonical NRF2 activators, such as p21 and apurinic/apyrimidinic endonuclease1 in A549 and acted oppositely in NCI–H23 cells. Additionally, NRF2-repressors, namely forkhead box O3, p53, glycogen synthase kinase-3β, and retinoid X receptor (RXR), were downregulated in NCI–H23 and upregulated in A549 cells. ICC exhibited that BT modulated the co-localization of NRF2 regulators, such as β-transducin repeat-containing protein and RXR, in A549 and NCI–H23 cells.</div></div><div><h3>Conclusion</h3><div>Therefore, it might be indicated that BT improved Dox retention and increased the Dox responsiveness in A549 cells. BT-mediated selective suppression of the NRF2, re-stabilized the KEAP-1-independent NRF2 regulators and made the non-responsive A549 cells partially sensitive to Dox.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 1","pages":"Article 101297"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01Epub Date: 2026-02-12DOI: 10.1016/j.jaim.2025.101291
S.J. Vidyavarsha , Bibu John Kariyil , Varsha Unni , R. Anoopraj
Background
Wound healing is a multifaceted biological process hindered by infections, especially the methicillin-resistant Staphylococcus aureus (MRSA), which resists antibiotics. Natural compounds such as andrographolide and hordenine are being explored as alternative therapeutics.
Objective
To compare the efficacy of andrographolide and hordenine in promoting wound healing in MRSA-infected infectious wounds using in vitro and in vivo methods.
Methods
L929 fibroblast-based MTT and scratch assays assessed cytotoxicity and wound closure potential. CAM assay evaluated angiogenic activity. Wistar rats with MRSA-infected excision wounds were treated topically, followed by biochemical analysis (hydroxyproline and hexosamine levels) and histopathological evaluation.
Results
The study revealed that andrographolide showed significantly (P < 0.001) higher cellular viability than hordenine and significantly reduced the cellular gap in L929 cells by day 3. Andrographolide increased blood vessels significantly (P < 0.001) more than hordenine. Clinical observations indicated reduced pus in andrographolide and hordenine-treated groups than in the control group, with the ceftriaxone-sulbactam group showing the lowest pus formation. Hexosamine levels were comparable in both treatments, while hydroxyproline was higher in the hordenine group. Histopathology showed hordenine-treated animals had faster wound healing and enhanced epithelialisation compared to andrographolide-treated animals.
Conclusion
Andrographolide and hordenine exhibited wound healing activity in MRSA-infected wounds, promotes fibroblast proliferation and migration, as well as fostering angiogenesis.
{"title":"Comparative efficacy of andrographolide and hordenine in the treatment of methicillin resistant Staphylococcus aureus infected wounds: a multifaceted approach","authors":"S.J. Vidyavarsha , Bibu John Kariyil , Varsha Unni , R. Anoopraj","doi":"10.1016/j.jaim.2025.101291","DOIUrl":"10.1016/j.jaim.2025.101291","url":null,"abstract":"<div><h3>Background</h3><div>Wound healing is a multifaceted biological process hindered by infections, especially the methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), which resists antibiotics. Natural compounds such as andrographolide and hordenine are being explored as alternative therapeutics.</div></div><div><h3>Objective</h3><div>To compare the efficacy of andrographolide and hordenine in promoting wound healing in MRSA-infected infectious wounds using <em>in vitro</em> and <em>in vivo</em> methods.</div></div><div><h3>Methods</h3><div>L929 fibroblast-based MTT and scratch assays assessed cytotoxicity and wound closure potential. CAM assay evaluated angiogenic activity. Wistar rats with MRSA-infected excision wounds were treated topically, followed by biochemical analysis (hydroxyproline and hexosamine levels) and histopathological evaluation.</div></div><div><h3>Results</h3><div>The study revealed that andrographolide showed significantly (P < 0.001) higher cellular viability than hordenine and significantly reduced the cellular gap in L929 cells by day 3. Andrographolide increased blood vessels significantly (P < 0.001) more than hordenine. Clinical observations indicated reduced pus in andrographolide and hordenine-treated groups than in the control group, with the ceftriaxone-sulbactam group showing the lowest pus formation. Hexosamine levels were comparable in both treatments, while hydroxyproline was higher in the hordenine group. Histopathology showed hordenine-treated animals had faster wound healing and enhanced epithelialisation compared to andrographolide-treated animals.</div></div><div><h3>Conclusion</h3><div>Andrographolide and hordenine exhibited wound healing activity in MRSA-infected wounds, promotes fibroblast proliferation and migration, as well as fostering angiogenesis.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 1","pages":"Article 101291"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146194585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01Epub Date: 2026-02-12DOI: 10.1016/j.jaim.2025.101290
Anjana Roy , Garima Srivastava , Medha S. Kulkarni , Anil Kumar , Rakesh Kumar Rana , Shivakumar S. Harti
Background
The prevalence of psychosomatic disorders associated with emotional instability has risen considerably in recent years. Among the different negative emotions, anger stands out as the most intense and prototypical. When excessive and poorly regulated, it serves as a significant contributing factor in the pathogenesis of various psychosomatic disorders.
Objective
This study aims to develop and systematically validate an integrated Ayurvedic Anger Assessment Scale (AAAS) for the adult population based on Ayurvedic, Indian philosophical, and contemporary psychological concepts, and this manuscript discusses its psychometric validation.
Materials & methods
An extensive literature review and expert consultations were conducted for item generation. A panel of 10 experts evaluated content and face validity. Subsequently, pre-testing was carried out with 15 healthy individuals to assess internal consistency and test–retest reliability. Construct validity was further assessed by administering the questionnaire to a sample of 105 individuals.
Results
Content Validity Index (I-CVI) of the items ranged from 0.50 to 1.00. Given that the panel consisted of ten experts, a minimum acceptable CVI value of 0.78 was applied. Accordingly, seven items with CVI values below this threshold were excluded, and the Scale-level Content Validity Index (S-CVI) was subsequently calculated, yielding a value of 0.91. The Content Validity Ratio (CVR) across items ranged from 0.20 to 1.00. Reliability analysis demonstrated strong internal consistency, with a Cronbach's alpha of 0.944. Test–retest reliability, assessed using the Pearson correlation coefficient, produced values ranging from 0.70 to 1.00 (p < 0.01) across all items. Exploratory factor analysis (EFA) using principal component analysis extracted nine components with eigenvalues >1.
Conclusion
This scale adopts an integrative approach to assessing the intensity of expressed anger, including negative consequences in the form of anger rumination. The development and validation process has been comprehensive, with significant psychometric properties inspiring future researchers to undertake similar studies.
{"title":"The Ayurvedic Anger Assessment Scale: An integrative approach for measuring anger in adults","authors":"Anjana Roy , Garima Srivastava , Medha S. Kulkarni , Anil Kumar , Rakesh Kumar Rana , Shivakumar S. Harti","doi":"10.1016/j.jaim.2025.101290","DOIUrl":"10.1016/j.jaim.2025.101290","url":null,"abstract":"<div><h3>Background</h3><div>The prevalence of psychosomatic disorders associated with emotional instability has risen considerably in recent years. Among the different negative emotions, anger stands out as the most intense and prototypical. When excessive and poorly regulated, it serves as a significant contributing factor in the pathogenesis of various psychosomatic disorders.</div></div><div><h3>Objective</h3><div>This study aims to develop and systematically validate an integrated Ayurvedic Anger Assessment Scale (AAAS) for the adult population based on Ayurvedic, Indian philosophical, and contemporary psychological concepts, and this manuscript discusses its psychometric validation.</div></div><div><h3>Materials & methods</h3><div>An extensive literature review and expert consultations were conducted for item generation. A panel of 10 experts evaluated content and face validity. Subsequently, pre-testing was carried out with 15 healthy individuals to assess internal consistency and test–retest reliability. Construct validity was further assessed by administering the questionnaire to a sample of 105 individuals.</div></div><div><h3>Results</h3><div>Content Validity Index (I-CVI) of the items ranged from 0.50 to 1.00. Given that the panel consisted of ten experts, a minimum acceptable CVI value of 0.78 was applied. Accordingly, seven items with CVI values below this threshold were excluded, and the Scale-level Content Validity Index (S-CVI) was subsequently calculated, yielding a value of 0.91. The Content Validity Ratio (CVR) across items ranged from 0.20 to 1.00. Reliability analysis demonstrated strong internal consistency, with a Cronbach's alpha of 0.944. Test–retest reliability, assessed using the Pearson correlation coefficient, produced values ranging from 0.70 to 1.00 (p < 0.01) across all items. Exploratory factor analysis (EFA) using principal component analysis extracted nine components with eigenvalues >1.</div></div><div><h3>Conclusion</h3><div>This scale adopts an integrative approach to assessing the intensity of expressed anger, including negative consequences in the form of anger rumination. The development and validation process has been comprehensive, with significant psychometric properties inspiring future researchers to undertake similar studies.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 1","pages":"Article 101290"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146194664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-01Epub Date: 2026-01-29DOI: 10.1016/j.jaim.2025.101269
Ramakrishna Allam , B. Kothainayagi , P.A. Sudhir , Vanitha R. Muralikumar , Muthuvel Arumugam , C.R. Rene Robin , S. Yokesh , U. Vishali , S. Fahima
The AYUSH sector relies mainly on medicinal plants and metals, which form the foundation of traditional healing practices. However, all plants are often inaccessible to many students due to geographic and seasonal limitations. To bridge this gap, Ayurveda Medical College, in collaboration with, Incubation Center and Engineering College, initiated a pilot project to develop a Virtual Herbal Garden a Solution for problem statement given by All India Institute of Ayurveda, under Smart India Hackathon 2024. This digital platform offers an immersive, interactive, and user-friendly learning experience, enabling users to explore medicinal plants in detail. The garden includes 3D models, multimedia resources, and comprehensive information on five medicinal plants: Cassia fistula, Ocimum sanctum, Aloe vera, Mentha piperita, and Azadirachta indica. The Virtual Herbal Garden is expected to become a valuable educational tool, promoting awareness and understanding of plants used in traditional medicine in the AYUSH sector.
{"title":"Virtual herbal garden (Hayushasutra): An educational aid for Ayurveda students","authors":"Ramakrishna Allam , B. Kothainayagi , P.A. Sudhir , Vanitha R. Muralikumar , Muthuvel Arumugam , C.R. Rene Robin , S. Yokesh , U. Vishali , S. Fahima","doi":"10.1016/j.jaim.2025.101269","DOIUrl":"10.1016/j.jaim.2025.101269","url":null,"abstract":"<div><div>The AYUSH sector relies mainly on medicinal plants and metals, which form the foundation of traditional healing practices. However, all plants are often inaccessible to many students due to geographic and seasonal limitations. To bridge this gap, Ayurveda Medical College, in collaboration with, Incubation Center and Engineering College, initiated a pilot project to develop a Virtual Herbal Garden a Solution for problem statement given by All India Institute of Ayurveda, under Smart India Hackathon 2024. This digital platform offers an immersive, interactive, and user-friendly learning experience, enabling users to explore medicinal plants in detail. The garden includes 3D models, multimedia resources, and comprehensive information on five medicinal plants: <em>Cassia fistula</em>, <em>Ocimum sanctum</em>, <em>Aloe vera</em>, <em>Mentha piperita</em>, and <em>Azadirachta indica</em>. The Virtual Herbal Garden is expected to become a valuable educational tool, promoting awareness and understanding of plants used in traditional medicine in the AYUSH sector.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 1","pages":"Article 101269"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Modern personalized medicine is transforming healthcare by customizing treatments based on individual genetic, environmental, and lifestyle profiles. However, its application remains complex and often technologically intensive. Traditional systems, particularly Persian Medicine (PM), offer distinctive frameworks that align with and can enrich contemporary personalized care. Unlike other traditional systems, PM is grounded in a unique philosophical concept of health governed by Nature (Tabi'at)—an intrinsic healing force—and operationalized through the concept of temperament (Mizaj). In this article, we systematically review both classical sources and contemporary clinical research to explore how PM offers structured diagnostic methods, preventive principles, and therapeutic modalities. We present a descriptive comparative overview of PM, Ayurveda, and Traditional Chinese Medicine (TCM) and provide practical models of integration into modern healthcare. We propose that PM provides a philosophically coherent and empirically grounded model that complements genomic medicine while fostering truly patient-centered, integrative care. However, the evidence base remains nascent, and further high-quality research is needed to validate its integrative potential.
{"title":"Toward a holistic model of personalized and integrative medicine: The distinctive role of Persian medicine","authors":"Yasaman Vazani MD, PhD , Babak Daneshfard MD, PhD , Majid Nimrouzi MD, MPH, PhD","doi":"10.1016/j.jaim.2025.101287","DOIUrl":"10.1016/j.jaim.2025.101287","url":null,"abstract":"<div><div>Modern personalized medicine is transforming healthcare by customizing treatments based on individual genetic, environmental, and lifestyle profiles. However, its application remains complex and often technologically intensive. Traditional systems, particularly Persian Medicine (PM), offer distinctive frameworks that align with and can enrich contemporary personalized care. Unlike other traditional systems, PM is grounded in a unique philosophical concept of health governed by Nature (<em>Tabi'at</em>)—an intrinsic healing force—and operationalized through the concept of temperament (<em>Mizaj</em>). In this article, we systematically review both classical sources and contemporary clinical research to explore how PM offers structured diagnostic methods, preventive principles, and therapeutic modalities. We present a descriptive comparative overview of PM, Ayurveda, and Traditional Chinese Medicine (TCM) and provide practical models of integration into modern healthcare. We propose that PM provides a philosophically coherent and empirically grounded model that complements genomic medicine while fostering truly patient-centered, integrative care. However, the evidence base remains nascent, and further high-quality research is needed to validate its integrative potential.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"17 1","pages":"Article 101287"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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