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Coexisting pilonidal sinus and anal fistula: Successful management with minimal invasive Kshara Karma Technique and Ksharasutra therapy– A case report 并发的毛突窦和肛瘘:微创Kshara Karma技术和Ksharasutra疗法的成功治疗-一例报告。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101082
P. Hemantha Kumar, Hetal Koriya
Pilonidal sinus (PNS) is a nest of hair which typically presents as pits, pus discharge, and an abscess at the natal cleft region. In rare conditions where pilonidal abscess is not drained properly, it progresses posteriorly and reaches anal canal, eventually coexisting with anal fistula. Both are associated with a high recurrence rate despite surgical management. A 27 year old female patient presented with complaints of non-healing wound in the natal cleft region and pus discharge from wound for 2 months with a past surgical history for PNS. On local examination, a wound was seen at the natal cleft, secondary pit 3cm away with scar mark of the previous operation, and a digital rectal examination (DRE) revealed an internal opening at 6 o'clock position that was communicating with the previously operated PNS wound. The patient was diagnosed as a case of coexisting anal fistula and recurrent PNS. Patient was managed under spinal anaesthesia with interception of fistulous tract using Ksharasutra (Medicated alkaline thread), followed by Minimal Invasive Kshara Karma Technique (MIKKT) for PNS. Ksharasutra was sloughed out after the fifth week, and complete wound healing of PNS was achieved in six weeks. By interception of the fistulous tract, the length of the tract was reduced, which facilitated early wound healing and minimal scar mark formation. MIKKT also prevented bigger excision and wound formation, eventually reducing complete healing duration, and Kshara(a medicated alkali) applied caused chemical cauterization and hence prevented recurrence of pilonidal sinus.
毛毛窦(PNS)是一窝毛,通常表现为坑,脓流,脓肿在出生的裂口区域。在极少数情况下,如果毛膜脓肿引流不当,它会向后发展并到达肛管,最终与肛瘘共存。尽管手术治疗,这两种疾病的复发率都很高。患者27岁,女,主诉先天性裂区创面未愈合,创面脓流2个月,既往有PNS手术史。在局部检查中,在出生裂处发现了一个伤口,在3cm处发现了先前手术的疤痕痕迹,直肠指检(DRE)显示在6点钟位置有一个内部开口,与先前手术的PNS伤口相通。该患者被诊断为并发肛瘘和复发性PNS病例。患者在脊髓麻醉下使用Ksharasutra(药物碱性线)阻断瘘道,随后采用微创Kshara Karma技术(MIKKT)治疗PNS。第5周后,Ksharasutra脱落,6周后,PNS伤口完全愈合。通过阻断瘘道,减少了瘘道的长度,促进了早期伤口愈合和最小疤痕形成。MIKKT还可以防止更大的切除和伤口形成,最终减少完全愈合时间,Kshara(一种药碱)的使用可以引起化学烧灼,从而防止毛毛窦的复发。
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引用次数: 0
Quo vadis, Ayurveda?
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101042
R.S. Rajan
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引用次数: 0
Effect of Ayurveda and Siddha interventions in the management of chronic lymphocytic leukemia: A case report 阿育吠陀和悉达疗法在慢性淋巴细胞白血病治疗中的效果:病例报告。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101017
Abhay Kumar Prajapati , Parvathy Venate , T. Monika , V.G. Huddar
Chronic Lymphocytic Leukemia (CLL) is a hematological malignancy marked by the clonal proliferation of the mature, yet dysfunctional B lymphocytes in blood and its subsequent infiltration of the marrow, lymphoid tissues and spleen. The resultant immune dysfunction exposes the body to multiple infections and is a cause of major mortality. Here, we discuss the case of a 46-year-old male suffering from CLL who presented with long-standing episodes of low-grade fever, productive cough and throat irritation, lethargy and pedal edema. He was treatment-naïve at the time of presentation. He was treated in lines of Rajayakshma as per Ayurveda, and Kuruthiputru as per Siddha medicine. After three years of treatment with continued follow up till date, comprising of Shamana Chikitsa (Ayurveda and Siddha internal medications), Sodhana (bio purificatory methods)through Mridu Virechana (therapeutic purgation) using Trivrut Avaleha 35g, Guduchi Rasayana (Tinospora cordifolia) and Yashtimadhu Rasayana(Glycyrrhiza glabra), patient reported reduction in frequency of infections, improved performance score with a mean reduction in TLC counts by 100 thousand/cu. mm in 10 months starting intervention. Presently the patient is stable with occasional episodes of dry cough and generalized weakness.
Integrated practices involving Ayurveda and Siddha medicines may be beneficial to patients suffering from CLL by reducing the episodes of infections, improving the quality of life while pacifying the deranged hematological parameters to certain extent.
慢性淋巴细胞白血病(CLL)是一种血液系统恶性肿瘤,其特征是成熟但功能失调的B淋巴细胞在血液中克隆性增殖,并随后浸润到骨髓、淋巴组织和脾脏。由此产生的免疫功能障碍使身体暴露于多种感染,是导致主要死亡的原因。在这里,我们讨论的情况下,46岁的男性患有慢性淋巴细胞白血病谁表现出长期发作低烧,生产咳嗽和喉咙刺激,嗜睡和脚水肿。他在演讲时是treatment-naïve。根据阿育吠陀的说法,他接受了拉贾雅什玛的治疗,根据悉达医学,他接受了库鲁蒂普特鲁的治疗。经过三年的持续随访治疗,包括Shamana Chikitsa(阿医吠陀和悉陀内部药物),Sodhana(生物净化方法)通过Mridu Virechana(治疗性净化)使用Trivrut Avaleha 35g, Guduchi Rasayana(Tinospora cordifolia)和Yashtimadhu Rasayana(glyglyrabrra),患者报告感染频率减少,性能评分提高,TLC计数平均减少10万/cu。在开始干预的10个月里。目前患者病情稳定,偶有干咳和全身无力。阿育吠陀和悉达药物的综合实践可能对慢性淋巴细胞白血病患者有益,减少感染的发作,提高生活质量,同时在一定程度上安抚紊乱的血液学参数。
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引用次数: 0
A trans-disciplinary agro-ecology strategy to grow medicinal plants 种植药用植物的跨学科农业生态学策略。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.100985
Atul K. Gupta , K Ravikumar , Venu Gopal , Noorunnisa Begum , Tabassum , Sathya Sangeetha , Pavithra Narendran , Darshan Shankar
The scope of the emerging field of Ayurvedic-biology visualized thus far is confined to studies on dimensions pertaining to clinical and experimental pharmacology, basic trans-disciplinary science and drug design. However, given the multiple facets of classical Ayurveda knowledge system, its application in the field of organic agriculture perhaps also needs to be urgently explored. The urgency is due to the growing public acceptance of Ayurveda as a preferred clinical choice for well-being and disease management. The turnover of the sector across manufacturing and health services is estimated to be around Rs.1,00,000 crores per annum. Medicinal plants today and in the coming decade will therefore be required in large volumes and given that their applications are solely for enhancing health of humans, livestock (Pashu Ayurveda) and crops (Vriksh Ayurveda), it is imperative that they be cultivated in an organic manner employing the fusion of best available inter-cultural knowledge.
The Ayurvedic subjects relevant for organic agriculture are Desh vichar, Dravya guna Shastra and Vriksh Ayurveda. From the perspective of modern biology subjects like soil micro biome, genetics, plant physiology and the natural geographical distribution of species are relevant.
It must be stated at the very outset that this article is largely theoretical. While experiments in Vriksh Ayurveda have been attempted on a small scale, the fusion of Ayurveda and biology for improving organic agriculture of medicinal plants has thus far not been systematically explored.
到目前为止,阿育吠陀生物学新兴领域的范围仅限于临床和实验药理学、基础跨学科科学和药物设计方面的研究。然而,鉴于经典阿育吠陀知识体系的多面性,其在有机农业领域的应用或许还需要迫切探索。这一紧迫性是由于越来越多的公众接受阿育吠陀作为健康和疾病管理的首选临床选择。据估计,整个制造业和卫生服务部门的营业额每年约为100亿卢比。因此,今天和未来十年将需要大量药用植物,鉴于它们的应用仅仅是为了增进人类、牲畜(Pashu Ayurveda)和作物(Vriksh Ayurveda)的健康,必须采用融合现有最佳跨文化知识的有机方式种植它们。与有机农业相关的阿育吠陀主题是Desh vichar, Dravya guna Shastra和Vriksh阿育吠陀。从现代生物学的角度来看,土壤微生物组、遗传学、植物生理学和物种的自然地理分布等学科都是相关的。必须在一开始就指出,这篇文章在很大程度上是理论性的。虽然Vriksh阿育吠陀的实验已经进行了小规模的尝试,但阿育吠陀和生物学的融合,以改善药用植物的有机农业,到目前为止还没有系统地探索。
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引用次数: 0
Interconnecting surgical science, art, philosophy and technology for holistic health
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101010
Sachin Hari Deshpande
Surgery is the branch of medicine which involves correction of the diseases by means of interventions and by means of various instruments and methods. Surgery in latin is called as Chirugi and in the Indian context it is termed as ‘Shalyatantra’. Surgery is the science as well as the tantra or technology. Ayurveda itself believes that, it is the technology by applying the word tantra to it. Shalyatantra is not only the technology, but it is the science with firm foundations; and the procedures or interventions involve the art within it. The philosophical insights from various Darshanas contributed to the derivation of foundational principles. The expert of surgery is called as ‘Surgeon’ and termed as ‘Shalyatantragya’ by Ayurveda. A surgeon has to master not only the science and art of surgical procedure; but also the philosophy, technology alongwith the details of medicines and drugs formulations. The procedures in surgery are the techniques, which have to be mastered by means of practice and by performing multiple operations. But this is not enough to become a good surgeon. The essential conditions for the expertise in surgery are, to master upon the surgical science and philosophy and to get acquainted with the advanced technologies also. In this particular article, the interconnections among surgical science, art, philosophy and technology and their applications are being discussed with special emphasis upon the ancient text of Sushruta, that is, Sushruta Samhita. Also it has been tried to critically analyse how the guiding principles from ancient Shalyatantra of Sushruta can be contributory to the surgery of the current medical practice.
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引用次数: 0
Dr. Muhammed Majeed: Father of Indian nutraceuticals industry- a life profile 穆罕默德·马吉德博士:印度营养药品工业之父——生平简介。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101074
Anju Majeed , Shaheen Majeed , Amirhossein Sahebkar
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引用次数: 0
Potentilla fulgens root extract rich in polyphenols ameliorate diabetic foot ulcers in wistar rats via regulating oxidative stress and connective tissue markers 富多酚的白陵草根提取物通过调节氧化应激和结缔组织标志物改善糖尿病大鼠足溃疡。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101075
Suman Kumar , Yogesh P. Nikam , Shatabdi Ghose , Susankar Kushari , Shailendra S. Gurav , Satyendra K. Prasad , Debasish Borbora , Damiki Laloo
Background: Potentilla fulgens (Wall.) ex Hook. (Rosaceae), commonly known as ‘Bajradanti’ is native to the lower Himalayan regions inclusive of the North-East India. Traditionally, the plant is used to treat chronic ailments like diabetes and diverse wounds, including gastric and mouth ulcers, and injuries from tiger bites. Objective: This study aims to assess the efficacy of P. fulgens root extract rich in polyphenolics in healing diabetic foot ulcer (DFU) in rats. Methods: DFU was induced in streptozotocin-nicotinamide diabetic rat feet by open excision wound model. Rats were divided into eight groups (n = 6/group): normal, negative control, plain gel (placebo) control, 1% silver sulfadiazine, and P. fulgens treated with topical ethyl acetate (EAPF, 5% and 10%) and methanol extract (MEPF, 5% and 10%). The morphological, histological and various biochemical parameters associated with the wound healing process such as connective tissue repair and oxidative stress biomarkers were evaluated. Results: Topical application of EAPF 10% and MEPF 10% over 21 days significantly (p < 0.05) reduced ulcer area in DFU rats compared to negative control. Furthermore, the extracts treated group notably (p < 0.05) increased levels of connective tissue biomarkers (total protein, hydroxyproline, hexosamine, and hexuronic acid) and antioxidant components (SOD and GSH), while significantly (p < 0.05) decreasing levels of lipid peroxidation (LPO) in foot tissue homogenate. Histological analysis of rats treated with the extracts revealed significant evidence of tissue repair, characterised by enhanced epithelial and collagen formation, along with decreased polymorphonuclear infiltration and edema. Conclusion: In summary, treatment with EAPF 10% significantly improved foot ulcers by modulating oxidative stress and connective tissue biomarkers in DFU animals.
背景:Potentilla fulgens (Wall.) ex Hook。(蔷薇科),通常被称为“Bajradanti”,原产于喜马拉雅山脉下游地区,包括印度东北部。传统上,这种植物被用来治疗慢性疾病,如糖尿病和各种伤口,包括胃溃疡和口腔溃疡,以及老虎咬伤。目的:探讨富多酚根提取物对大鼠糖尿病足溃疡(DFU)的治疗作用。方法:采用链脲唑素-烟酰胺致糖尿病大鼠足切开创面模型诱导DFU。将大鼠分为8组(n = 6/组):正常组、阴性对照组、普通凝胶(安慰剂)对照组、1%磺胺嘧啶银组和外用乙酸乙酯(EAPF, 5%和10%)和甲醇提取物(MEPF, 5%和10%)处理的富尔根杆菌。评估与伤口愈合过程相关的形态学、组织学和各种生化参数,如结缔组织修复和氧化应激生物标志物。结果:局部应用10% EAPF和10% MEPF超过21天显著(p)结论:综上所述,10% EAPF治疗通过调节DFU动物的氧化应激和结缔组织生物标志物显著改善足溃疡。
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引用次数: 0
An experimental and computational approach to evaluate the antidiabetic activity of Commiphora wightii gum extract 用实验和计算方法评价白花金缕梅提取物的抗糖尿病活性。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101038
Shalini Jain , Mukesh Kumar Sharma , Nidhi Gupta , Jivanage Anirudh , Hemanth Naick Banavath , Sreemoyee Chatterjee

Background

Plant formulations with antidiabetic and antioxidant properties have recently gained popularity due to their lower cost and lesser side effects. Guggul gum is one such formulation that is extensively being used to cure various ailments.

Objective

The present study was designed to explore the antioxidant and antidiabetic properties of the aqua-ethanolic Guggul gum extract (GE) from Commiphora wightii using in silico studies and in vitro assays.

Methods

Gas Chromatography Mass Spectroscopy (GCMS) identified compounds were docked to the Human pancreatic α-amylase (HPA, PDB ID: 1HNY) for in silico studies to predict the inhibition. Molecular dynamics simulations (MDS) were performed using GROMACS for 100 ns. The inhibition of the enzyme was further evaluated at in vitro level to show the compounds' hypoglycemic role.

Results

The extract showed a good amount of phenolic (5.14 ± 0.011 mg), flavonoid (0.66 ± 0.023 mg) and terpenoid (1.08 ± 0.018 mg) content along with a promising free radical scavenging activity of 41.96 ± 4.02%. In the in silico studies, 3 out of 6 GCMS-identified bioactive compounds showed permissible values of bioavailability properties suggesting them as a potential candidate for antidiabetic drugs. Similarly, in molecular docking studies, 3 compounds showed more binding energy than the standard drug acarbose indicating better inhibition. MDS studies showed Compound 4 (Diisooctyl phthalate), was the most stable with the lowest root mean square deviation (RMSD) and root mean square fluctuation (RMSF) values, a consistent radius of gyration (Rg), and stable solvent accessible surface area (SASA). This was further confirmed by in vitro analysis where the pancreatic α-amylase inhibitory activity of the extract and the standard drug (acarbose) were comparable at an IC50 value of 4.17 ± 1.26 mg/mL and 3.69 ± 0.89 mg/mL respectively.

Conclusion

The results demonstrated GE as a potential alternative to commercial antidiabetic drugs. Out of the major 6 GCMS-identified compounds, Compound 4 showed the most stable conformation during MDS studies. However, the isolation of the identified compounds could be done in the future for in vivo studies.
背景:具有抗糖尿病和抗氧化特性的植物制剂由于其较低的成本和较小的副作用而受到欢迎。Guggul口香糖就是这样一种配方,被广泛用于治疗各种疾病。目的:采用硅片法和体外实验法,探讨白花龙葵水乙醇胶提取物(GE)的抗氧化和抗糖尿病作用。方法:用气相色谱-质谱法(GCMS)将鉴定的化合物与人胰腺α-淀粉酶(HPA, PDB ID: 1HNY)对接,进行硅质化研究,预测其抑制作用。使用GROMACS进行100 ns的分子动力学模拟(MDS)。在体外水平进一步评估酶的抑制作用,以显示化合物的降糖作用。结果:提取液中酚类化合物(5.14±0.011 mg)、类黄酮(0.66±0.023 mg)、萜类化合物(1.08±0.018 mg)含量较高,对自由基的清除能力为41.96±4.02%。在计算机研究中,6种gcms鉴定的生物活性化合物中有3种显示出允许的生物利用度值,表明它们是抗糖尿病药物的潜在候选者。同样,在分子对接研究中,有3种化合物比标准药物阿卡波糖显示出更高的结合能,表明具有更好的抑制作用。MDS研究表明,化合物4(邻苯二甲酸二异辛酯)稳定,均方根偏差(RMSD)和均方根波动(RMSF)值最低,旋转半径(Rg)一致,溶剂可及表面积(SASA)稳定。体外分析进一步证实了这一点,提取物和标准药物(阿卡波糖)的胰腺α-淀粉酶抑制活性相当,IC50值分别为4.17±1.26 mg/mL和3.69±0.89 mg/mL。结论:GE是一种潜在的抗糖尿病药物替代品。在gcms鉴定的6个主要化合物中,化合物4在MDS研究中表现出最稳定的构象。然而,鉴定的化合物的分离可以在未来进行体内研究。
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引用次数: 0
Add on effect of Whole System Ayurveda protocol in suboptimal controlled Primary Hypothyroidism - A randomized controlled trial 全系统阿育吠陀方案对亚理想控制原发性甲状腺功能减退的影响-一项随机对照试验。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101052
Vivek , Arun Chougale , Himanshu Joshi , Basavaraj R. Tubaki

Background

Primary hypothyroidism (PH) is a common chronic life style disorder. Whole system Ayurveda protocol on PH (WSAPH) is explored for it's possible complimentary role in the management of suboptimal controlled PH on stable levothyroxine therapy.

Objective

To evaluate the efficacy of whole system Ayurveda protocol on Primary hypothyroidism with suboptimal response to stable levothyroxine therapy.

Material and methods

Study was a randomized controlled trial. Total 46 known patients of suboptimal response to stable levothyroxine therapy, age group 20–60 years of either sex participated in the study. They were randomly placed in two groups, Group KG received Kanchanara guggulu two tablets of 500 mg each, thrice a day with water and group WSAPH was on whole system Ayurveda protocol for primary hypothyroidism (WSAPH). Interventions were for 60 days. Assessments were done through various parameters like Thyroid profiles (TSH, T3, T4, Free T4), Weight, BMI, Skin fold thickness (SFT), Body fat index (BFI), Waist hip ratio (WHR), Lipid profile, Zulewskis clinical score for hypothyroidism (ZUL), WHO Quality of Life- BREF (WHOQOL-BREF), Clinical Global Improvement scales (CGI). Clinical assessments were done on every 15th day and biochemical were done before and after interventions.

Results

Study showed that WSAPH group produced significant outcome improvement compared to KG group in TSH (p = 0.02), weight (p = 0.02), SFT (p = 0.04), BFI (p = 0.01), WHOQOL-BREF (p = 0.004) and ZUL (p = 0.02). Both the group showed improvements in BMI, WHR, BFI, SFT, WHOQOL-BREF, ZUL, CGI scales. Effect size was medium in TSH, weight, BFI, SFT and ZUL.

Conclusion

WSAPH was effective in management of PH and normalized TSH and ZUL scores in patients with suboptimal response to stable levothyroxine therapy. Further studies are needed.
背景:原发性甲状腺功能减退症(PH)是一种常见的慢性生活方式障碍。探讨阿育吠陀PH全系统方案(wwsh)在稳定左甲状腺素治疗控制PH次优的管理中可能发挥的补充作用。目的:评价阿育吠陀全系统治疗原发性甲状腺功能减退症对稳定左甲状腺素治疗反应不佳的疗效。材料与方法:研究为随机对照试验。共有46例已知对稳定左甲状腺素治疗反应不佳的患者,年龄20-60岁,男女不限。随机分为两组,KG组患者给予Kanchanara gugugululu 2片,每片500 mg,每日3次,加水治疗;wwsh组患者采用全系统阿育吠陀治疗原发性甲状腺功能减退(wwsh)。干预期为60天。通过各种参数进行评估,如甲状腺谱(TSH、T3、T4、游离T4)、体重、BMI、皮肤厚度(SFT)、体脂指数(BFI)、腰臀比(WHR)、脂质谱、Zulewskis甲状腺功能减退临床评分(ZUL)、WHO生活质量-BREF (WHOQOL-BREF)、临床总体改善量表(CGI)。每15天进行一次临床评估,干预前后进行生化检查。结果:与KG组比较,wwsh组在TSH (p = 0.02)、体重(p = 0.02)、SFT (p = 0.04)、BFI (p = 0.01)、WHOQOL-BREF (p = 0.004)、ZUL (p = 0.02)方面均有显著改善。两组BMI、WHR、BFI、SFT、WHOQOL-BREF、ZUL、CGI量表均有改善。TSH、体重、BFI、SFT和ZUL的效应量为中等。结论:在对稳定左甲状腺素治疗反应不佳的患者中,wwsh可有效管理PH、TSH和ZUL标准化评分。需要进一步的研究。
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引用次数: 0
Promotion of cellular differentiation and DNA repair potential in brain cancer cells by Shankhpushpi, (Clitoria ternatea L.) with rasayana properties in vitro Shankhpushpi, (Clitoria ternatea L.)具有rasayana特性的体外促进脑癌细胞分化和DNA修复潜力。
IF 1.7 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2025-01-01 DOI: 10.1016/j.jaim.2024.101050
Keshava Prasad , Kothanahalli S. Raghu , Swathi Maruthiyodan , Renu Wadhwa , Sunil C. Kaul , Kapaettu Satyamoorthy , Kanive Parashiva Guruprasad

Background

Brain ageing is accompanied by the diminution of neuronal plasticity, which is correlated with the inability to respond to loss of memory, various stress-induced stimuli, and increased risk of neurodegenerative disorders. In the recent past, plant based herbal medicines are of interest over synthetic drugs for therapeutic purposes due to lower side effects. The Indian traditional medicine Ayurveda describes several herbal remedies, such as rasayana (elixirs for rejuvenation), to treat many age-related diseases. Medhya rasayana (brain elixir) is a class of rasayana used for its nootropic functions, such as enhancement of memory and intellect, in addition to promoting normal health. Clitoria ternatea L. is one such plant used in the preparation of medhya rasayana.

Objective

To investigate the neuronal differentiation and DNA repair potential of Shankhpushpi (Clitoria ternatea L.) in neuroblastoma cells.

Materials & methods

The effect of Clitoria ternatea L. on neuronal cell differentiation, DNA repair (base excision repair, comet, γH2AX immunostaining assays), autophagy by cadaverine uptake and mitochondrial functions by fluorescent dye staining through flow cytometry were evaluated.

Results

The results revealed that Clitoria ternatea L. enhanced DNA repair and mitochondrial membrane potential and reduced autophagy and reactive oxygen species (ROS) in IMR-32 neuroblastoma cells. Treatment of IMR-32 neuroblastoma and C6 glioblastoma cells with shankhpushpi induced neuronal differentiation and exhibited markers such as MAP2, Mortalin and GFAP.

Conclusion

Neurobiological pathways and molecular mechanisms influenced by rasayana herb shankhpushpi suggests its therapeutic potential for neurodegenerative diseases.
背景:大脑老化伴随着神经元可塑性的降低,这与记忆丧失、各种应激性刺激和神经退行性疾病风险增加的反应能力相关。在最近的过去,植物性草药由于副作用更小,在治疗目的上比合成药物更受关注。印度传统医学阿育吠陀(Ayurveda)描述了几种草药疗法,如rasayana(返老还童的灵丹妙药),可以治疗许多与年龄有关的疾病。Medhya rasayana(大脑灵丹妙药)是一类rasayana,用于其益智功能,如增强记忆和智力,除了促进正常健康。阴蒂是一种用于制作中草药的植物。目的:探讨山普皮在神经母细胞瘤细胞中的分化及DNA修复潜能。材料与方法:观察阴蒂对神经元细胞分化、DNA修复(碱基切除修复、彗星、γ - h2ax免疫染色法)、尸胺摄取法自噬和流式细胞术荧光染色法线粒体功能的影响。结果:阴蒂可增强IMR-32神经母细胞瘤细胞的DNA修复和线粒体膜电位,降低细胞自噬和活性氧(ROS)水平。shankhpushpi治疗IMR-32神经母细胞瘤和C6胶质母细胞瘤细胞可诱导神经元分化,并表现出MAP2、Mortalin和GFAP等标志物。结论:香蒲皮影响神经生物学通路和分子机制,提示其治疗神经退行性疾病的潜力。
{"title":"Promotion of cellular differentiation and DNA repair potential in brain cancer cells by Shankhpushpi, (Clitoria ternatea L.) with rasayana properties in vitro","authors":"Keshava Prasad ,&nbsp;Kothanahalli S. Raghu ,&nbsp;Swathi Maruthiyodan ,&nbsp;Renu Wadhwa ,&nbsp;Sunil C. Kaul ,&nbsp;Kapaettu Satyamoorthy ,&nbsp;Kanive Parashiva Guruprasad","doi":"10.1016/j.jaim.2024.101050","DOIUrl":"10.1016/j.jaim.2024.101050","url":null,"abstract":"<div><h3>Background</h3><div>Brain ageing is accompanied by the diminution of neuronal plasticity, which is correlated with the inability to respond to loss of memory, various stress-induced stimuli, and increased risk of neurodegenerative disorders. In the recent past, plant based herbal medicines are of interest over synthetic drugs for therapeutic purposes due to lower side effects. The Indian traditional medicine Ayurveda describes several herbal remedies, such as rasayana (elixirs for rejuvenation), to treat many age-related diseases. Medhya rasayana (brain elixir) is a class of rasayana used for its nootropic functions, such as enhancement of memory and intellect, in addition to promoting normal health. <em>Clitoria ternatea</em> L. is one such plant used in the preparation of medhya rasayana.</div></div><div><h3>Objective</h3><div>To investigate the neuronal differentiation and DNA repair potential of Shankhpushpi (<em>Clitoria ternatea</em> L.) in neuroblastoma cells.</div></div><div><h3>Materials &amp; methods</h3><div>The effect of <em>Clitoria ternatea</em> L. on neuronal cell differentiation, DNA repair (base excision repair, comet, γH2AX immunostaining assays), autophagy by cadaverine uptake and mitochondrial functions by fluorescent dye staining through flow cytometry were evaluated.</div></div><div><h3>Results</h3><div>The results revealed that <em>Clitoria ternatea</em> L. enhanced DNA repair and mitochondrial membrane potential and reduced autophagy and reactive oxygen species (ROS) in IMR-32 neuroblastoma cells. Treatment of IMR-32 neuroblastoma and C6 glioblastoma cells with shankhpushpi induced neuronal differentiation and exhibited markers such as MAP2, Mortalin and GFAP.</div></div><div><h3>Conclusion</h3><div>Neurobiological pathways and molecular mechanisms influenced by rasayana herb shankhpushpi suggests its therapeutic potential for neurodegenerative diseases.</div></div>","PeriodicalId":15150,"journal":{"name":"Journal of Ayurveda and Integrative Medicine","volume":"16 1","pages":"Article 101050"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Journal of Ayurveda and Integrative Medicine
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