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The Classification Characteristics and Dynamic Changes of Desert Vegetation Based on Unmanned Aerial Vehicle Remote Sensing 基于无人机遥感的沙漠植被分类特征与动态变化
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2316
Kun Yue, Penghui Li
The common ground sample method of vegetation survey requires a lot of manpower and time. Meanwhile, many vegetation information may not be revealed because the scale of standard sample is usually only 100 m2. For this reason, this study developed a set of desert vegetation sample survey method based on UAV technology, and further developed a set of vegetation spatial distribution pattern analysis method based on it, and carried out an empirical study with typical shrub communities in Inner Mongolia as an example. It is expected to provide scientific basis for wind and sand control, vegetation restoration and ecological construction in the ecologically fragile area at the edge of Inner Mongolia.
常用的地面样地植被调查方法需要耗费大量的人力和时间。同时,由于标准样品的尺度通常只有100 m2,因此可能无法揭示许多植被信息。为此,本研究开发了一套基于无人机技术的荒漠植被样本调查方法,并在此基础上进一步开发了一套植被空间分布格局分析方法,并以内蒙古典型灌木群落为例进行了实证研究。为内蒙古边缘生态脆弱区风沙治理、植被恢复和生态建设提供科学依据。
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引用次数: 0
Unveiling Oil Seed Cakes Ability to Suppress Fusarium Wilt (Fusarium udum Butler) in Pigeonpea (Cajanus cajan L. Millsp.) 揭示油籽饼对鸽子豆(Cajanus cajan L. Millsp.)镰刀菌枯萎病(Fusarium udum Butler)的抑制能力
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2319
Amit Kumar Maurya, Aditya, Vinny John, Hemlata Pant, Sujata P. Sharma, Doaa Z. El-Refaey, R. Sami, M. Helal, Fadi Baakdah, Nevin Ahmed
Pigeonpea (Cajanus cajan) holds significant economic importance as a versatile crop due to its various uses. It is a valuable source of nutrition for human consumption, rich in protein, dietary fiber, vitamins and minerals. Additionally, pigeonpea plays a crucial role in sustainable agriculture by fixing atmospheric nitrogen and enhancing soil fertility. Fusarium wilt caused by Fusarium udum is an important economic threat to the growers of pigeonpea in India and across the globe. The study was therefore conducted during the years 2017–2019 to determine the impact of the various oil seed cakes on the fusarium wilt of pigeonpea. Seven treatments including different types of oil seed cakes viz., mustard cake, neem cake, linseed cake, mahua cake, sunflower cake, groundnut cake and carbendazim (treated check) were employed by adopting the soil inoculation method. All of the treatments employed notably diminished the incidence of fusarium wilt. Among the treatments, neem cake (T2) was most effective in suppressing disease incidence (73.78% and 76.89%), registering the least plant fatalities (19.05 and 18.02), respectively. Furthermore, treatment (T2) also showed maximum plant height at 120 DAS (151.86 cm and 153.34 cm) and the highest root-nodules count of pigeon pea (5.71, 5.66) respectively in both the cropping years compared to other treatments and untreated control (T0).
鸽豆(Cajanus cajan)由于其多种用途,作为一种多功能作物具有重要的经济意义。它是人类食用的宝贵营养来源,富含蛋白质、膳食纤维、维生素和矿物质。此外,鸽子豆通过固定大气氮和提高土壤肥力在可持续农业中起着至关重要的作用。由镰刀菌引起的枯萎病是对印度乃至全球鸽豆种植者的重要经济威胁。因此,该研究在2017-2019年期间进行,以确定各种油籽饼对鸽子豌豆枯萎病的影响。采用土壤接种法对不同类型油籽饼、芥菜饼、楝树饼、亚麻籽饼、麻花饼、向日葵饼、花生饼和多菌灵(处理对照)进行处理。所有处理均显著降低了枯萎病的发病率。其中,印楝饼(T2)抑制病害发生率最高(73.78%和76.89%),植株死亡率最低(分别为19.05和18.02)。与其他处理和未处理对照(T0)相比,T2处理(T2)在两个种植年份的最大株高为120 DAS (151.86 cm和153.34 cm),根瘤数最高(5.71、5.66)。
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引用次数: 0
Determination and Method Optimization of Lianjie Liyan Compound Mixture 连洁丽妍复合混合物的测定与方法优化
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2337
Min Jiang, Yin Lin, Jinghua Li, Haoran Yang, Yingquan Fang
Euchresta japonica, Platycodon grandiflorum, Belamcanda chinensis, and Adenophora tetraphylla are the four medicinal substances that make up the Lianjie Liyan compound mixture, a type of compound preparation for treating throat diseases such as pharyngitis and laryngeal carcinoma. The preparation has been used clinically for a long time and has been proven that it has curative properties in terms of alleviating pain and other symptoms. However, no quality control research on this preparation has been done yet. The pharmacological components in the main quality control standards, including Oxymatrine, matrine, N-methylcytisine, platycoside-D in Platycodon grandiflorum, and irisaxanthin in Shegan, were analyzed to further standardize the clinically rational use of this preparation and prevent the occurrence of adverse reactions and side effects. In this study, the five active components in the mixture were determined concurrently for the first time with the help of Box-Behnken design and response surface approach. The properties of established model showed excellent resolution and sensitivity, good reproducibility and speed, and accurate prediction thanks to the optimization of HPLC measurement conditions. The established approach was precise and trustworthy, and it offered helpful support and references for the construction of the Lianjie Liyan compound mixture as well as for the quality control.
连节理炎复方合剂是治疗咽炎、喉癌等咽喉疾病的复方制剂,是由真丝、桔梗、白桦、四叶参组成的四种药用物质。该制剂已在临床上使用了很长时间,并已被证明在减轻疼痛和其他症状方面具有疗效。但目前尚未对该制剂进行质量控制研究。对主要质量控制标准中的药理学成分,桔梗中的氧化苦参碱、苦参碱、n -甲基胱氨酸、桔梗中的桔梗苷- d、蛇柑中的鸢尾黄素进行分析,进一步规范该制剂在临床上的合理使用,防止不良反应和副作用的发生。在本研究中,首次采用Box-Behnken设计和响应面法同时测定了混合物中的五种有效成分。通过对HPLC测定条件的优化,所建立的模型具有良好的分辨率和灵敏度,重现性和速度好,预测准确等特点。所建立的方法准确可靠,为连节理炎复方合剂的配制及质量控制提供了有益的支持和参考。
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引用次数: 0
Characteristics of Pseudomonas stutzeri Oxidation of As(III)/Sb(III) 石炭酸假单胞菌氧化 As(III)/Sb(III) 的特征
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2331
Miao Tian, Linfeng Jin, Zhouyou Xu, Erke Zhang, Biyu Li, Xinhui Deng, Liang Chen
Heavy metal pollution, especially arsenic (As) and antimony (Sb) pollution, seriously threatens people’s health and the ecological system. It is a common occurrence for As and Sb to coexist in the environment as inorganic species, including trivalent/pentavalent As or trivalent/pentavalent Sb. In addition, trivalent As and trivalent Sb are more toxic and difficult to remove than pentavalent As and pentavalent Sb. Therefore, the simultaneous pre-oxidation of trivalent As and trivalent Sb to pentavalent As and pentavalent Sb is a very critical step in the process of pollution control. A strain of Pseudomonas stutzeri, by which trivalent As/trivalent Sb can be oxidized to pentavalent As/pentavalent Sb, was selected, and its oxidation characteristics were studied in detail. The oxidation mechanism was thoroughly analyzed through a series of experiments and characterization. The results indicated that Pseudomonas stutzeri could tolerate trivalent As (577 mg/L) and trivalent Sb (1752 mg/L); the highest oxidation ratio of trivalent As was 53.05% and that of trivalent Sb was 83.05%. According to the cytotoxicity assay, the largest inhibition ratio was 89.22% for trivalent As and 74.98% for trivalent Sb under 5 mM of trivalent As/trivalent Sb stress. In addition, the minimum inhibitory concentrations of trivalent As and trivalent Sb were 0.22 mM and 0.43 mM, respectively. The results of Raman and FTIR spectroscopy analyses revealed that the peak vibration intensity of all strains under trivalent As and trivalent Sb stress was weaker than that of the control group. Therefore, it could be concluded that the toxicity of trivalent As for Pseudomonas stutzeri is greater than that of trivalent Sb according to the maximum concentration tolerated by Pseudomonas stutzeri and the minimum inhibitory concentrations. The oxidation ratio of trivalent Sb for Pseudomonas stutzeri is higher than that of trivalent As, with both trivalent As and trivalent Sb being bonded to the organic groups. The simultaneous oxidation of Sb(III) and As(III) by Pseudomonas stutzeri suggests its potential as a candidate for bioremediation of environments contaminated with these elements. This study provides a theoretical basis and guidance for the microbial oxidation of trivalent As and trivalent Sb.
重金属污染,特别是砷、锑污染,严重威胁着人们的健康和生态系统。As和Sb作为无机物质在环境中共存是很常见的,包括三价/五价As或三价/五价Sb。此外,三价As和三价Sb比五价As和五价Sb毒性更大,更难去除。因此,将三价As和三价Sb同时预氧化为五价As和五价Sb是污染控制过程中非常关键的一步。选择了一株能将三价As/三价Sb氧化为五价As/五价Sb的stutzeri假单胞菌,并对其氧化特性进行了研究。通过一系列实验和表征,对氧化机理进行了深入分析。结果表明:斯图氏假单胞菌可耐受三价砷(577 mg/L)和三价锑(1752 mg/L);三价砷和三价锑的最高氧化率分别为53.05%和83.05%。结果表明,在5 mM的三价砷/三价锑胁迫下,对三价砷和三价锑的抑制率分别为89.22%和74.98%。此外,三价As和三价Sb的最低抑菌浓度分别为0.22 mM和0.43 mM。拉曼光谱和FTIR光谱分析结果表明,三价As和三价Sb应力下各应变的峰值振动强度均弱于对照组。由此可见,从史图氏假单胞菌的最大耐受浓度和最小抑制浓度来看,三价As对史图氏假单胞菌的毒性大于三价Sb。三价Sb对stutzeri假单胞菌的氧化率高于三价As,因为三价As和三价Sb都与有机基团结合。stutzeri假单胞菌对Sb(III)和As(III)的同时氧化表明其作为被这些元素污染的环境的生物修复候选物的潜力。本研究为微生物氧化三价砷和三价锑提供了理论依据和指导。
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引用次数: 0
Effects of Ammonia Nitrogen, Nitrite and pH on Embryo In Vitro Incubation of Red Crayfish 氨氮、亚硝酸盐和 pH 值对红螯虾胚胎体外孵化的影响
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2324
Jianguo Wang, Gang Li, Yongjun Bao
As an important economic aquatic animal, red crayfish’s reproduction and incubation can affect its breeding efficiency. With aquaculture developing, the study of factors affecting aquaculture species growth has gradually become an important research direction. To study the effects of ammonia nitrogen, nitrite and pH on red crayfish embryos, the In Vitro incubation experiment of red crayfish embryos was conducted. The residual module was used to design the embryo counting method of red crayfish. And different ammonia nitrogen, nitrite and PH gradients were set to analyze the hatching rate and emergence rate of red crayfish’s embryo development.When conducting research method performance testing, the training loss value of the research method decreased to 84 after 200 iterations. On the 6th day in an ammonia nitrogen environment, the number of water microbiota was only 4.74 M. The emergence rate of red crayfish decreases to a lower level when the ammonia nitrogen concentration is above 0.6 mg/L. The emergence rate of red crayfish decreased with the increase of nitrite concentration, and decreased to 0 when the nitrite concentration reached 1.25 mg/L. When the pH value of water body is 8.1, the emergence rate of red crayfish reaches the highest value of 68.92%. Ammonia nitrogen and nitrite concentration will inhibit the In Vitro hatching of crayfish embryos, and the appropriate PH value can improve the emergence rate of crayfish hatching In Vitro.
作为一种重要的经济水生动物,红螯虾的繁殖和孵化影响其繁殖效率。随着水产养殖的发展,对水产养殖物种生长影响因素的研究逐渐成为一个重要的研究方向。为研究氨氮、亚硝酸盐和pH对红螯虾胚胎的影响,进行了红螯虾胚胎体外培养实验。利用残差模块设计了红螯虾胚胎计数方法。设置不同的氨氮、亚硝酸盐和PH梯度,分析红螯虾胚胎发育的孵化率和出苗率。在进行研究方法性能测试时,经过200次迭代,研究方法的训练损失值下降到84。在氨氮环境下第6天,水体微生物群数量仅为4.74 m,当氨氮浓度高于0.6 mg/L时,红螯虾的出苗率下降到较低水平。随着亚硝酸盐浓度的增加,红螯虾的出苗率降低,当亚硝酸盐浓度达到1.25 mg/L时,红螯虾的出苗率降至0。当水体pH值为8.1时,红螯虾出苗率最高,达68.92%。氨氮和亚硝酸盐浓度会抑制小龙虾胚胎的体外孵化,适当的PH值可以提高小龙虾体外孵化的出苗率。
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引用次数: 0
Nutritional and Sensory Quality Evaluation of Low-Temperature Yogurt Sold in the Market Based on Principal Component Analysis and Cluster Analysis 基于主成分分析和聚类分析的市场上销售的低温酸奶的营养和感官质量评价
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2317
Siran Zhang
In order to explore the quality differences of various brands of low-temperature yogurt sold in the market, the nutritional components and physical characteristics of 11 kinds of stirred lowtemperature commercial yogurt were analyzed by principal component analysis (PCA), including protein content, total sugar content, fat content, sodium content, calcium content, probiotic content, solid content, pH value, L*, a*, b*, viscosity and whey precipitation rate. In addition, combined with the method of sensory evaluation, the quality evaluation model is initially constructed. The results show that the cumulative variance contribution rate of the four principal components extracted by principal component analysis method reaches 90.74%. Based on the comprehensive evaluation function of principal component analysis method, the calculated results are significantly positively correlated with the sensory evaluation results (r = 0.909, P <0.001), which reflects the high reliability and effectiveness of the evaluation model. By cluster analysis, 11 kinds of yogurt can be divided into four groups. The first group is characterized by high protein and mellow taste, the second group is characterized by balanced nutrition and popular taste, the third group is characterized by no additive and original flavor, and the fourth group is characterized by moderate quality in all aspects. The research results provide a theoretical basis for the quality evaluation of stirred low-temperature yogurt and the research and development of fermented dairy products.
为了探究市场上销售的不同品牌低温酸奶的品质差异,采用主成分分析法(PCA)对11种搅拌低温商品酸奶的营养成分和物理特性进行了分析,包括蛋白质含量、总糖含量、脂肪含量、钠含量、钙含量、益生菌含量、固体含量、pH值、L*、a*、b*、粘度和乳清沉淀率。并结合感官评价方法,初步构建了质量评价模型。结果表明,主成分分析法提取的4个主成分的累积方差贡献率达到90.74%。基于主成分分析法的综合评价函数,计算结果与感官评价结果显著正相关(r = 0.909, P <0.001),反映了评价模型的高可靠性和有效性。通过聚类分析,将11种酸奶分为4类。第一组以高蛋白、口感醇厚为特点,第二组以营养均衡、口味通俗为特点,第三组以无添加剂、原味为特点,第四组以各方面品质适中为特点。研究结果为搅拌低温酸奶的质量评价和发酵乳制品的研究开发提供了理论依据。
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引用次数: 0
Effect of a Self-Made Herbal Decoction Containing Angelica sinensis Combined with an Enhanced Recovery After Surgery Nursing Model on Anxiety, Depression, and Ovarian Function in Patients Undergoing Myomectomy 含当归的自制草药煎剂与术后康复护理模式相结合对子宫肌瘤切除术患者焦虑、抑郁和卵巢功能的影响
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2332
Xiaomei Dong, Yunjiao Zheng
Angelica sinensis (A. sinensis), a traditional Chinese medicine (TCM), can tonify the blood, promote circulation, and nourish the body. This study investigates the potential of A. sinensis extract in improving ovarian function at the molecular level and its combined effects with other natural medicinal herbs in patients undergoing myomectomy. The impact of A. sinensis extract was assessed using a rat model of premature ovarian insufficiency. The rats were divided into four groups: control group, model group, low-dose Angelica group, and high-dose Angelica group. Serum levels of estradiol (E2), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were determined, and the pathology of ovarian tissue was examined. The results revealed elevated expressions of E2, nuclear factor erythroid 2-related factor 2 (Nrf-2), and heme oxygenase-1 (HO-1) in the treated groups compared to the model group, along with decreased levels of LH, FSH, interleukin (IL)-4, IL-6, and tumor necrosis factor-α. In a clinical trial involving patients after myomectomy, the efficacy of a combination of TCM decoction containing A. sinensis and enhanced recovery after surgery nursing model was evaluated with anxiety, depression, and ovarian function as indicators. The observation group exhibited reduced levels of anxiety and depression, along with improved ovarian function compared to the control group. Moreover, it was observed that A. sinensis extract demonstrated the potential in enhancing ovarian function through molecular pathways, possibly involving Nrf-2/HO-1 signaling. These findings provide insights into novel therapeutic approaches for promoting ovarian health and emphasize the benefits of integrating traditional medicine with modern care strategies.
当归(a . sinensis)是一种传统的中药(TCM),可以补血,促进循环,滋养身体。本研究从分子水平探讨了五倍子提取物改善子宫肌瘤切除术患者卵巢功能的潜力及其与其他天然草药的联合作用。采用卵巢功能不全大鼠模型,评价了白荆提取物的作用。将大鼠分为4组:对照组、模型组、当归低剂量组、当归高剂量组。测定血清雌二醇(E2)、促黄体生成素(LH)、促卵泡生成素(FSH)水平,并检查卵巢组织病理学。结果显示,与模型组比较,治疗组E2、核因子-红细胞2相关因子2 (Nrf-2)、血红素加氧酶-1 (HO-1)表达升高,LH、FSH、白细胞介素(IL)-4、IL-6、肿瘤坏死因子-α水平降低。在一项子宫肌瘤切除术后患者的临床试验中,以焦虑、抑郁、卵巢功能为指标,评价中药含五倍子汤剂联合术后增强恢复护理模式的疗效。与对照组相比,观察组的焦虑和抑郁水平有所降低,卵巢功能也有所改善。此外,我们还观察到紫荆提取物可能通过Nrf-2/HO-1信号通路等分子途径增强卵巢功能。这些发现为促进卵巢健康的新治疗方法提供了见解,并强调了将传统医学与现代护理策略相结合的好处。
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引用次数: 0
Practical Effect of Cervical Softening Decoction Combined with Delivery Ball Approach in Promoting Vaginal Delivery in Primiparous Women 宫颈软化煎剂结合分娩球法促进初产妇阴道分娩的实际效果
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2326
Shanshan Sun, Zhen Ye, Wenjun Wang
In Traditional Chinese Medicine, Cervical Softening Decoction is derived from a modified “Wan Bing Hui Chun” formulation and consists of natural herbal ingredients such as Angelica sinensis, Ligusticum chuanxiong, Leonurus heterophyllus, and Cortex Daphnes. The decoction regulates Qi, promotes blood circulation, and facilitates fetus descent. Genkwanin is an active compound in L. heterophyllus and previous studies identified this compound in ethanolic extracts from rat uterine smooth muscle cell membrane fractions. To further understand the pharmacological activities of Cervical Softening Decoction, we examined genkwanin effects on isolated rat uterine contractions and protein expression in rat tissue with postpartum hemorrhage complicated by multiple organ dysfunction syndrome (MODS). We also explored the practical effects of Cervical Softening Decoction combined with a birthing ball approach on promoting natural delivery in primiparous women. First, an isolated rat uterine smooth muscle contraction model was generated and three groups established: oxytocin group (0.002 U/mL), conventional genkwanin dose group (3 μg/mL), and high genkwanin dose group (6.0 μg/mL). Changes in uterine smooth muscle contraction and relaxation amplitudes were recorded before and after administration. Second, 50 rats were used to establish the following groups: (1) control group, (2) postpartum hemorrhage with MODS model group (MODS group), and (3) a postpartum hemorrhage with MODS treated with genkwanin group (genkwanin group). Western blotting was used to detect and compare tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 protein expression levels in lung tissue from groups. Finally, 84 primiparous women admitted to our hospital between January 2020 and December 2021 were selected and divided into two groups based on their obstetric interventions. The control group (n = 42) received routine obstetric intervention, while the observation group (n = 42) received Cervical Softening Decoction and delivery ball interventions. Delivery conditions were compared across groups. In basic studies, genkwanin doses significantly increased contraction and relaxation values in isolated rat uterine smooth muscle (P <0.05). Moreover, the genkwanin contractile effects at conventional and high doses were comparable with oxytocin. TNF-α and IL-6 protein expression in MODS group lung tissue was significantly higher when compared with the control group, while expression in the genkwanin group was significantly reduced when compared with the MODS group (P <0.05). In clinical studies, the observation group showed significantly higher natural delivery rates and Labor Agentry Scale (LAS) scores when compared with the control group. In contrast, cesarean section rates, first and second stage labor duration, total labor duration, and Facial Pain Scale (FPS) scores were significantly lower in the observation group when compared with the control group (P <0.05). In basic studies,
在中医中,颈柔汤是由“万兵会春”配方改良而成,由当归、川芎、益母草、达芙妮等天然草药成分组成。该汤调气活血,促进胎儿下降。根管万素是一种有效化合物,已有研究在大鼠子宫平滑肌细胞膜的乙醇提取物中发现该化合物。为了进一步了解颈椎软化汤的药理作用,我们研究了根宽素对产后出血并发多器官功能障碍综合征(MODS)大鼠离体子宫收缩和组织蛋白表达的影响。我们还探讨了宫颈软化汤联合分娩球法促进初产妇自然分娩的实际效果。首先建立离体大鼠子宫平滑肌收缩模型,建立催产素组(0.002 U/mL)、根管万素常规剂量组(3 μg/mL)、根管万素高剂量组(6.0 μg/mL) 3组。记录给药前后子宫平滑肌收缩舒张幅度的变化。其次,选用50只大鼠,建立3组:(1)对照组,(2)产后出血MODS模型组(MODS组),(3)产后出血MODS治疗组(genkwanin组)。采用免疫印迹法检测各组肺组织中肿瘤坏死因子-α (TNF-α)和白细胞介素(IL)-6蛋白的表达水平并进行比较。最后,选择2020年1月至2021年12月期间入住我院的84名孕妇,并根据产科干预措施分为两组。对照组(n = 42)给予常规产科干预,观察组(n = 42)给予宫颈软化汤和分娩球干预。各组间比较分娩情况。在基础研究中,根宽万素剂量显著增加离体大鼠子宫平滑肌收缩舒张值(P <0.05)。此外,在常规剂量和高剂量下,根管万素的收缩作用与催产素相当。MODS组肺组织中TNF-α、IL-6蛋白表达量较对照组显著升高,根管万素组肺组织中TNF-α、IL-6蛋白表达量较MODS组显著降低(P <0.05)。在临床研究中,观察组的自然分娩率和分娩代理量表(LAS)评分均明显高于对照组。观察组剖宫产率、第一、第二产程、总产程、面部疼痛量表(FPS)评分均显著低于对照组(P <0.05)。在基础研究中,根宽万素对离体大鼠子宫平滑肌有明显的收缩作用,并能有效抑制产后出血合并MODS大鼠的炎症反应。在临床研究中,宫颈软化汤联合分娩球在促进初产妇阴道分娩方面取得了很好的实际效果。干预显著提高了自然分娩的概率,缩短了分娩时间,缓解了产妇疼痛,增强了产妇对分娩的控制。
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引用次数: 0
Changes in Nitrogen and Humus During Aerobic Composting Under Antibiotic Composite Pollution 抗生素复合污染下好氧堆肥过程中氮和腐殖质的变化
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2343
Lulu Zhang, Yingying Sun, Qiao Zeng, Jing Wang, Yulu Wei
Antibiotic residues have significantly effects on aerobic composting process. This study was targeted at antibiotic composite pollutants (e.g., sulfanilamides, tetracyclines, and quinolones) and probed into the effects of pollution levels on aerobic composting. With higher initial antibiotic concentrations, the NH4+ –N and NO3− –N concentrations at the end of composting were higher, but TN content decreased, which may be due to the inhibition effects of antibiotics on nitrogen-fixing bacteria and thus were unfavorable for nitrogen accumulation. Antibiotic pollution obviously delayed composting maturity, and the antibiotics at initial concentration above 306.12 mg/kg were significantly toxic to seeds. Analysis of structural changes in dissolved organic matter (DOM) showed the humification after treatments CK and AT100 mainly occurred at the high-temperature stage (day 2–14) and later lower-temperature maturity stage (day 21–36). Humification after treatment AT300 mainly happened at the high-temperature stage (day 2–14), and that in AT600 mainly occurred at the early temperature rise and high-temperature stage (day 2–7). This study comprehensively evaluated the effect of antibiotic composite pollution on the maturity of aerobic compost, and provided a theoretical basis for the treatment of antibiotic composite pollution materials in practical production.
抗生素残留对好氧堆肥过程有显著影响。本研究针对抗生素复合污染物(如磺胺类、四环素类、喹诺酮类),探讨污染水平对好氧堆肥的影响。初始抗生素浓度越高,堆肥后期NH4+ -N和NO3−-N浓度越高,TN含量越低,这可能是由于抗生素对固氮菌的抑制作用,不利于氮素积累。抗生素污染明显延迟堆肥成熟,初始浓度大于306.12 mg/kg的抗生素对种子的毒性显著。可溶性有机质(DOM)结构变化分析表明,CK和AT100处理腐殖质化主要发生在高温期(2 ~ 14 d)和较晚的低温成熟期(21 ~ 36 d)。处理AT300后腐殖质化主要发生在高温阶段(2 ~ 14 d),处理AT600腐殖质化主要发生在升温前期和高温阶段(2 ~ 7 d)。本研究综合评价了抗生素复合污染对好氧堆肥成熟度的影响,为实际生产中抗生素复合污染材料的处理提供了理论依据。
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引用次数: 0
Genome Survey Sequencing of the Medical Plant Dioscorea futschauensis: Genomic Characteristics Estimation and Genomic Resources Development 药用植物 Dioscorea futschauensis 的基因组调查测序:基因组特征估计和基因组资源开发
IF 0.5 4区 医学 Pub Date : 2023-12-01 DOI: 10.1166/jbmb.2023.2351
Bizhen Luo, Dong Li, Ke Hu, Min Chen, Xiaoqin Sun, Ruisen Lu, Shujing Wei
Dioscorea futschauensis Uline ex R. Knuth, a perennial climbing herb belonging to the Stenophora section of the genus Dioscorea L. (Dioscoreaceae), originates from Southeast China and is highly regarded for its medicinal properties. Despite its medicinal significance, the absence of genomic information has impeded the effective utilization and conservation of this species. Here, we conducted genome survey sequencing of D. futschauensis to unveil its genomic characteristics and identify species-specific genomic markers, including whole plastome sequence, plastome-divergent hotspots, single-copy nuclear genes and polymorphic nuclear SSRs. The genome of D. futschauensis was approximated to be 1392.76 Mb in length, accompanied by a substantial heterozygosity rate of 1.75% and a repeat content of 67.43%. Comparative plastome analysis illuminated the conserved genome structure and gene content within D. futschauensis, and revealed four highly divergent hotspots (atpF intron, psbA-matK, matK -rps16, and rrn16-trnI) that could serve as molecular markers for D. futschauensis and its closely related species. Furthermore, more than 200 single-copy nuclear genes and 78 polymorphic nuclear SSRs were identified within D. futschauensis. Overall, the comprehensive genomic information and abundant genetic resources provided herein will not only facilitate extended research in the realms of population genetics, phylogenetics and conservation biology of D. futschauensis, but also hold considerable value for its cultivation and molecular breeding applications.
山药(Dioscorea futschauensis Uline ex R. Knuth)是山药科山药属(Dioscorea L.)的一种多年生攀援草本植物,原产于中国东南部,因其独特的药用价值而备受重视。尽管其具有重要的药用价值,但基因组信息的缺乏阻碍了该物种的有效利用和保护。本研究通过对福山雪豹进行基因组调查测序,揭示福山雪豹基因组特征,鉴定种特异性基因组标记,包括全质体序列、质体分化热点、单拷贝核基因和多态核ssr。futschauensis基因组长度约为1392.76 Mb,杂合率为1.75%,重复数为67.43%。对比质组分析揭示了该物种保守的基因组结构和基因含量,揭示了4个高度分化的热点(atpF内含子、psbA-matK、matK -rps16和rrn16-trnI),可作为该物种及其近缘种的分子标记。此外,还鉴定出200多个单拷贝核基因和78个多态核ssr。综上所述,本文所提供的全面的基因组信息和丰富的遗传资源,不仅有利于在狐猴种群遗传学、系统遗传学和保护生物学等领域的深入研究,而且对狐猴的培育和分子育种应用具有重要价值。
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Journal of Biobased Materials and Bioenergy
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