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Clinical Efficacy and Nursing Efficiency Analysis of Jiuwei Zhusheng Decoction in Promoting Cervical Ripening in the Perinatal Midwifery Holistic Care 九味竹笙煎剂在围产期助产整体护理中促进宫颈成熟的临床疗效及护理效率分析
IF 0.5 4区 医学 Pub Date : 2024-03-01 DOI: 10.1166/jbmb.2024.2360
Jinjin Liu, Fang Wu
To evaluate the clinical efficacy of Jiuwei Zhusheng Decoction (JWZSD) and perinatal midwifery holistic nursing care in promoting cervical ripening, we conducted a randomized controlled trial involving 80 primiparous and full-term pregnant women with indications for labor induction. The participants were divided into a control group (n = 40) receiving intravenous oxytocin for labor induction and a study group (n = 40) receiving oral administration of JWZSD. Additionally, 30 female Wistar pregnant rats were randomly assigned to the blank group, oxytocin group, and traditional Chinese medicine (TCM) group to compare relevant indicators. In the clinical study, both groups showed significant increases in Bishop scores after treatment. However, the study group demonstrated significantly greater improvements compared to the control group. Moreover, the study group exhibited higher rates of clinical effectiveness and successful vaginal delivery than the control group. In the animal experiment, both the oxytocin and TCM groups displayed disorganized and relaxed cervical connective tissue with evident interstitial edema when compared to the blank group. Furthermore, rats in the TCM group exhibited significantly elevated serum levels of interleukin-8 (IL-8), matrix metalloproteinase-9 (MMP-9), prostaglandin E2 (PGE2), and cyclooxygenase-2 (COX-2) compared to those in the oxytocin group. The combination of JWZSD and perinatal midwifery holistic nursing care for labor induction can effectively enhance cervical ripening and reduce the incidence of cesarean section, potentially through the regulation of IL-8, MMP-9, COX-2, and PGE2 expression. However, further investigations are required to elucidate the underlying mechanisms.
为了评估九味竹生煎(JWZSD)和围产期助产整体护理在促进宫颈成熟方面的临床疗效,我们进行了一项随机对照试验,涉及80名有引产指征的初产妇和足月孕妇。参与者被分为接受静脉催产素引产的对照组(40 人)和口服 JWZSD 的研究组(40 人)。此外,30 只雌性 Wistar 怀孕大鼠被随机分配到空白组、催产素组和中药组,以比较相关指标。在临床研究中,两组治疗后的 Bishop 评分均有显著提高。但与对照组相比,研究组的改善幅度明显更大。此外,研究组的临床有效率和阴道分娩成功率均高于对照组。在动物实验中,与空白组相比,催产素组和中药组都显示出宫颈结缔组织紊乱和松弛,间质水肿明显。此外,与催产素组相比,中药组大鼠血清中的白细胞介素-8(IL-8)、基质金属蛋白酶-9(MMP-9)、前列腺素 E2(PGE2)和环氧化酶-2(COX-2)水平明显升高。JWZSD与围产期助产整体护理联合应用于引产,可有效促进宫颈成熟,降低剖宫产率,这可能是通过调节IL-8、MMP-9、COX-2和PGE2的表达实现的。然而,要阐明其潜在机制还需要进一步的研究。
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引用次数: 0
Molecular Mechanisms of Phyllanthus urinaria in Cerebral Ischemia-Reperfusion Injury 尿囊素在脑缺血再灌注损伤中的分子机制
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2350
Deyuan Qin, Jiangcun Wei, Xiumei Ma, Bing Qing, Meiyan Qiu, Peng Yang, Zhengteng Yang
Phyllanthus urinaria (P. urinaria), a prominent herb in traditional Zhuang medicine, is characterized by its heat clearance, detoxification, diuresis, and detumescence. This study investigates the therapeutic effects and molecular mechanisms of P. urinaria on cerebral ischemia-reperfusion injury (CIRI). 60 adult SD rats were randomly divided into four groups: a sham operation group, a model group of right middle cerebral artery occlusion (MCAO), a low-dose group treated with P. urinaria at 5 g/kg, and a high-dose group treated with P. urinaria at 10 g/kg, each consisting of 15 rats. After seven days of continuous administration, a rat model of the right MCAO was established to simulate CIRI. Nerve function was assessed using the Longa scoring method, the cerebral infarction area was evaluated with TTC staining, and cytokine levels (IL-1β, TNF-α, IL-6) were measured via ELISA. PI3K and AKT protein expression in brain tissues was analyzed by Western blot. The results revealed a significant improvement in neurological function scores in both low and high-dose P. urinaria groups compared to the MCAO model group. Treatment with P. urinaria led to a notable reduction in cerebral infarction area after 72 hours of cerebral ischemia. The levels of inflammatory cytokines (IL-1β, TNF-α, IL-6) were decreased in both treatment groups compared to the MCAO model group. There was a significant increase in the expression of PI3K and AKT proteins in both treatment groups. In conclusion, P. urinaria exhibits a protective effect against CIRI by inhibiting inflammatory responses, improving neurological function, and reducing brain injury.
壮医药中的一味名贵药材--泌尿草,具有清热、解毒、利尿、消肿的功效。本研究探讨了荨麻对脑缺血再灌注损伤(CIRI)的治疗作用和分子机制。将 60 只成年 SD 大鼠随机分为四组:假手术组、右侧大脑中动脉闭塞(MCAO)模型组、5 克/千克 P. urinaria 低剂量组和 10 克/千克 P. urinaria 高剂量组,每组 15 只。连续给药七天后,建立右侧 MCAO 大鼠模型,模拟 CIRI。用 Longa 评分法评估神经功能,用 TTC 染色法评估脑梗塞面积,并通过 ELISA 检测细胞因子水平(IL-1β、TNF-α、IL-6)。通过 Western 印迹分析了脑组织中 PI3K 和 AKT 蛋白的表达。结果显示,与 MCAO 模型组相比,低剂量和高剂量尿囊素组的神经功能评分均有明显改善。在脑缺血 72 小时后,P. urinaria 能明显缩小脑梗死面积。与 MCAO 模型组相比,两个治疗组的炎症细胞因子(IL-1β、TNF-α、IL-6)水平均有所下降。两个治疗组中 PI3K 和 AKT 蛋白的表达均有明显增加。总之,P. urinaria 通过抑制炎症反应、改善神经功能和减轻脑损伤对 CIRI 具有保护作用。
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引用次数: 0
Effect of Steam Explosion on the Structure, Chemical Composition and Pyrolytic Volatile Composition of Tobacco Waste 蒸汽爆炸对烟草废料结构、化学成分和热解挥发物成分的影响
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2353
Ze Liu, Gaoxiang Xu, Chenhao Song, Wen Zuo, Siyuan Li, Chao Gui, Na Liu, Peng Hu, Ji Yang, Guofu Zhou, Wei Zhang, Yubao Chen
Tobacco stems are an important part of the tobacco leaf, containing a lot of cellulose, result in the low utilization of tobacco leaves in cigarettes. The pre-treatment technology of tobacco stems is a key to increase the utilization of tobacco stems. In this work, steam explosion treatment was carried out on tobacco stems with different moisture contents containing 2%, 5% and 10%, respectively. The steam explosion is carried out under different pressure and pressure holding time conditions, the changes in physical structure, chemical composition and pyrolysis volatiles of tobacco stem was investigated. The results indicate that steam explosion has a negligible impact on the crystalline structure of cellulose and low-order polysaccharide crystals, while it has an obvious effect on KCl. Steam explosion increases the specific surface area by destroying the amorphous structure on tobacco stems. The contents of chemical constituents and pyrolytic volatiles indicate that there is a significant leaching effect of lignin, but no change in cellulose content. Soluble polysaccharides, the main component of hemicellulose, were converted into pyrolytic volatiles e.g., furfural and furfuryl alcohol. In conclusion, the technology of steam explosion promotes the leaching, decomposition and chemical transformation of amorphous structures on tobacco stems, thus destroying the structure of the cell walls.
烟梗是烟叶的重要组成部分,含有大量纤维素,导致烟叶在卷烟中的利用率较低。烟梗预处理技术是提高烟梗利用率的关键。本研究对含水量分别为 2%、5% 和 10%的烟梗进行了汽爆处理。在不同压力和保压时间条件下进行汽爆,研究了烟梗物理结构、化学成分和热解挥发物的变化。结果表明,汽爆对纤维素和低阶多糖晶体的结晶结构影响微乎其微,而对氯化钾有明显影响。蒸汽爆炸破坏了烟草茎秆上的无定形结构,从而增加了比表面积。化学成分和热解挥发物的含量表明,木质素有明显的浸出作用,但纤维素含量没有变化。半纤维素的主要成分可溶性多糖转化为热解挥发物,如糠醛和糠醇。总之,汽爆技术促进了烟草茎秆无定形结构的浸出、分解和化学转化,从而破坏了细胞壁的结构。
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引用次数: 0
Cleaning Process of Diosgenin from Dioscorea nipponica 从薯蓣中提取薯蓣皂苷的清洁工艺
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2335
Jinwei Liu, Weichang Xu, Feng Yan, Xinqi Liu, Yuan Wang
This investigation employed HPLC and LC-MS techniques to elucidate the enzymolysis and acid hydrolysis mechanisms of diosgenin obtained through a cleaning process. The findings revealed that the enzymolysis led to the cleavage and subsequent recombination of the glycosidic bond at the C-26 position of protodioscin, resulting in the formation of dioscin present in the enzymatic hydrolysis filter residue. Leveraging this observation, a streamlined and eco-friendly method for diosgenin extraction was devised. Incorporating the Box-Behnken response surface methodology alongside wastewater assessment, the optimal parameters for the cleaning process were established: a sulfuric acid concentration of 3 mol · L−1, a solid–liquid ratio of 1:10, an acid hydrolysis temperature of 100 °C, and an acid hydrolysis duration of 3 h. Under these parameters, the yield and purity of diosgenin were 31.07±0.56% and 72.30±0.24% respectively. When benchmarked against the direct acid hydrolysis approach, there was an increase of 133.08% in diosgenin yield, 44.08% enhancement in diosgenin purity, 50% reduction in wastewater generation and acid utilization, and an 83.57% decrease in wastewater’s chemical oxygen demand (COD). This optimized cleaning process is viable for large-scale production and offers a sustainable method for diosgenin production.
这项研究采用高效液相色谱和液相色谱-质谱(LC-MS)技术,阐明了通过清洁工艺获得的薯蓣皂苷的酶解和酸水解机制。研究结果表明,酶解导致原薯蓣皂苷 C-26 位糖苷键的裂解和随后的重组,从而在酶水解过滤残留物中形成薯蓣皂苷。根据这一观察结果,我们设计出了一种提取薯蓣皂苷的简化且环保的方法。通过箱-贝肯响应面方法和废水评估,确定了清洗工艺的最佳参数:硫酸浓度为 3 mol - L-1,固液比为 1:10,酸水解温度为 100 °C,酸水解持续时间为 3 小时。与直接酸水解法相比,该方法的薯蓣皂苷产率提高了 133.08%,薯蓣皂苷纯度提高了 44.08%,废水产生量和酸利用率降低了 50%,废水的化学需氧量(COD)降低了 83.57%。这种优化的清洁工艺可用于大规模生产,并提供了一种可持续的薯蓣皂苷生产方法。
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引用次数: 0
Analysis of the Therapeutic Effect of Shengmai Yin Chinese Herbal Medicine in Pneumonia 生脉饮中药对肺炎的疗效分析
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2336
Lili Wang, Shoucong Yi
Health education is an important method to improve patients’ cognitive level and quality of life. Shengmai Yin (SMY) contain a variety of active ingredients of Chinese herbal medicine, pathology effect is relatively broad, plays an important role in a variety of chronic diseases treatment. This study aims to analyze the effect of nursing health education combined with the treatment with SMY on elderly patients with community-acquired pneumonia. Meanwhile, pharmaceutical information regarding the chemical composition, pharmacological effects, and pharmacokinetic characteristics of SMY in traditional Chinese medicine was summarized and analyzed. Animal experiments were conducted to analyze the immunomodulatory effects of SMY on elderly community acquired pneumonia. A total of 80 elderly patients with community-acquired pneumonia admitted to our hospital from July 2019 to July 2022 were selected as the research objects. According to different nursing models, they were divided into a control group and a study group, with 40 cases in each group. Patients in the control group were given routine nursing, while patients in the study group were given health education nursing and SMY treament on the basis of the control group. The overall differences were compared between the two groups. In rat experiment, a rat model of chronic bronchitis was used and divided into control, model control group, low-dose group, medium-dose group, and high-dose group according to the dosage. The levels of immunoglobulins (IgA, IgG, IgM), and cytokines (IL-1, IL-6, TNF-α) in rat serum were determined using ELISA. The disease knowledge, the quality of life score and the effect of health education of the study group was better than that of the control group (P < 0.05), the temperature remission time of the two groups was not significantly different (P > 0.05), but the respiratory rate remission time and cough remission time were shorter than that of the control group (P < 0.05). In rat experiment, The levels of IgA, IgG, IgM, IL-1, IL-6, and TNF-α in the serum of the model control group were higher than those in the normal control group. Compared with those in the model control group, the low-dose group exhibited lower levels of IgG, IgM, and IL-1, whereas the medium- and high-dose groups exhibited lower levels of IgA, IgG, IgM, IL-1, IL-6, and TNF-α (all P < 0.05). Health education and SMY treatment have a good impact on elderly patients with community-acquired pneumonia, which can effectively improve the patients’ mastery of health knowledge, treatment effect and quality of life. In addition, SMY had a significant downregulating effect on immunity and cytokines, providing anti-infective, antiallergic, and immunomodulatory effects, and is hence effective in treating bronchitis.
健康教育是提高患者认知水平和生活质量的重要方法。生脉散(SMY)含有多种中药有效成分,病理作用较为广泛,在多种慢性疾病治疗中发挥着重要作用。本研究旨在分析护理健康教育结合生脉饮治疗老年社区获得性肺炎患者的效果。同时,总结和分析了SMY在中药中的化学成分、药理作用和药代动力学特点等药物信息。通过动物实验分析了SMY对老年社区获得性肺炎的免疫调节作用。选取我院2019年7月-2022年7月收治的社区获得性肺炎老年患者共80例作为研究对象。根据不同的护理模式将其分为对照组和研究组,每组各40例。对照组患者给予常规护理,研究组患者在对照组基础上给予健康教育护理和SMY治疗。比较两组的总体差异。在大鼠实验中,采用慢性支气管炎大鼠模型,按剂量分为对照组、模型对照组、低剂量组、中剂量组、高剂量组。用酶联免疫吸附法测定大鼠血清中免疫球蛋白(IgA、IgG、IgM)和细胞因子(IL-1、IL-6、TNF-α)的水平。研究组的疾病知识、生活质量评分和健康教育效果均优于对照组(P<0.05),两组体温缓解时间无显著差异(P>0.05),但呼吸频率缓解时间和咳嗽缓解时间均短于对照组(P<0.05)。在大鼠实验中,模型对照组血清中的 IgA、IgG、IgM、IL-1、IL-6 和 TNF-α 水平高于正常对照组。与模型对照组相比,低剂量组的 IgG、IgM 和 IL-1 水平较低,而中剂量组和高剂量组的 IgA、IgG、IgM、IL-1、IL-6 和 TNF-α 水平较低(均 P <0.05)。健康教育和SMY治疗对老年社区获得性肺炎患者有较好的疗效,能有效提高患者对健康知识的掌握程度、治疗效果和生活质量。此外,SMY 对免疫和细胞因子有明显的下调作用,具有抗感染、抗过敏和免疫调节作用,因此对治疗支气管炎有一定疗效。
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引用次数: 0
An Evaluation of the Contribution of Cellulose, Hemicellulose and Lignin to Bio-Char Combustion and Adsorption Properties 评估纤维素、半纤维素和木质素对生物炭燃烧和吸附特性的贡献
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2338
Bing Dai, Zhaoguo Ding
The present work focuses on the contribution of cellulose, hemicellulose, and lignin for bio-char application in two aspects: fuels and adsorbent. The pyrolysis behaviors were studied at 300 to 700 °C, and the yields and characterization of bio-chars were compared. The results showed that in the three components, lignin was responsible for the formation of bio-char products, representing the main source of bio-char characterized by high heating value. Lignin was the strongest support for prepared bio-char as fuel decided by physico-chemical properties and combustion reactivity, while cellulose was the major contributor for bio-char products as ignition fuel. Scanning electron microscopy (SEM) and surface area analysis of bio-char showed that, in the three components, cellulose derived bio-chars exhibited a distinctive fibrous structure and the surface area was the largest at the same pyrolysis temperature. Compared with lignin, hemicellulose was a better precursor for adsorption, it is mainly determined by complex and abundant pore structure. Further adsorption tests of toxic substances in soil showed that, cellulose represented major adsorption donator for phenol, all bio-char samples had better adsorption capacity for Pb(II) than Cu(II), the rule on adsorption capacity of three different precursors for Cu(II) was as follows: cellulose>hemicellulose>lignin, for Pb(II): hemicellulose> cellulose> lignin.
本研究的重点是纤维素、半纤维素和木质素对生物炭在燃料和吸附剂两方面应用的贡献。研究了在 300 至 700 °C 下的热解行为,并比较了生物炭的产量和特性。结果表明,在三种成分中,木质素是形成生物炭产品的主要成分,是生物炭的主要来源,具有高热值的特点。从物理化学性质和燃烧反应性来看,木质素是制备生物炭作为燃料的最有力支持,而纤维素则是生物炭产品作为点火燃料的主要贡献者。生物炭的扫描电子显微镜(SEM)和表面积分析表明,在三种成分中,纤维素衍生的生物炭表现出独特的纤维状结构,在相同的热解温度下,其表面积最大。与木质素相比,半纤维素是一种更好的吸附前体,这主要是由复杂而丰富的孔隙结构决定的。对土壤中有毒物质的进一步吸附测试表明,纤维素是苯酚的主要吸附捐献者,所有生物炭样品对铅(II)的吸附能力均优于铜(II),三种不同前驱体对铜(II)的吸附能力规律为:纤维素>半纤维素>木质素,对铅(II)的吸附能力规律为:半纤维素>纤维素>木质素。
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引用次数: 0
Anomaly Detection in Species Distribution Patterns: A Spatio-Temporal Approach for Biodiversity Conservation 物种分布模式的异常检测:保护生物多样性的时空方法
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2340
Mingyang He, Hao Chen
Monitoring and maintaining biodiversity are crucial for the ecological balance and overall health of ecosystems. Detecting anomalies in species distribution patterns is essential for identifying areas of concern and implementing timely conservation measures. This paper introduces an approach to detect anomalies in spatiotemporal species activity data by integrating time series analysis, machine learning techniques, and spatial statistics. Our method identifies distribution patterns of various species and groups regions with similar patterns, enabling the segmentation of the study area into distinct categories. Within these categorized regions, we apply a combination of clustering algorithms and outlier detection techniques to pinpoint anomalous behaviors in species distribution. In order to confirm the reliability of our findings, we cross-reference them with verified data acquired through field observations or other credible data sources. These corroborations indicate that anomalies are frequently indicative of sudden variations in species numbers or unexpected alterations in spatial distribution at certain places and times. To gain a more robust understanding of how species are distributed, we curate a data set that excludes these anomalous observations and use it to develop a predictive algorithm. Our analysis shows that a predictive model trained on this refined, anomaly-free dataset achieves a lower normalized mean square error (NMSE), which suggests a higher level of predictive accuracy as compared to a model trained on data containing anomalies. Utilizing this methodology can facilitate the creation of effective conservation plans and contribute to more sustainable ecosystem management.
监测和维护生物多样性对生态系统的生态平衡和整体健康至关重要。检测物种分布模式中的异常现象对于确定关注区域和及时实施保护措施至关重要。本文介绍了一种通过整合时间序列分析、机器学习技术和空间统计来检测时空物种活动数据异常的方法。我们的方法可以识别各种物种的分布模式,并将具有相似模式的区域分组,从而将研究区域划分为不同的类别。在这些分类区域内,我们结合使用聚类算法和离群点检测技术,找出物种分布的异常行为。为了确认研究结果的可靠性,我们将研究结果与通过实地观察或其他可靠数据来源获得的验证数据进行交叉对比。这些佐证表明,异常现象往往表明物种数量的突然变化,或者在某些地点和时间的空间分布发生了意想不到的变化。为了更深入地了解物种是如何分布的,我们整理了一个数据集,排除了这些异常观测数据,并利用它开发了一种预测算法。我们的分析表明,与在包含异常数据的数据集上训练的模型相比,在这个经过提炼、不包含异常数据的数据集上训练的预测模型实现了更低的归一化均方误差(NMSE),这表明其预测准确性达到了更高水平。利用这种方法可以促进制定有效的保护计划,有助于实现更可持续的生态系统管理。
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引用次数: 0
Bee Pollen: An Antioxidant and Antimicrobial Agent for Beef Sausage 蜂花粉:牛肉香肠的抗氧化剂和抗菌剂
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2339
I. M. Taha, K. A. Ismail, R. Sami, Huda Aljumayi, G. Alshehry, Eman Algarni, Adel Aljumayi, Mohammad Y. Alshahrani, Tahani Y. A. Asseri, Zayed Alsharari, Fadi Baakdah, El-Kazafy A. Taha
Natural additives can enhance the microbial and oxidative quality of processed meat products. Bee pollen is a natural ingredient with valuable nutritional properties. It has been suggested as a natural antimicrobial and antioxidant agent. The goal of this research was to include bee pollen in beef sausage formulation as an antimicrobial and antioxidant agent for improving the quality criteria under cold storage. Five beef sausage formulas were prepared; the first formula was prepared from the basic formula (positive control) include butylatedhydroxytoluene (BHT), the second formula (negative control) was prepared like the basic formula without BHT and the other three formulations were prepared by including 1, 2 and 3% eucalyptus (Eucalyptus ssp.) bee pollen. All formulations were stored at 5±2 °C until spoilage symptoms appeared. Chemical composition, thiobarbituric acid reactive substance (TBA-RS), total volatile basic nitrogen (TVB-N), microbial counts and sensory properties were evaluated. The obtained results were compared with Egyptian criteria to assess their acceptability. In comparison with the sausage basic formula, formulas treated with BHT and bee pollen showed a significant reduction in TVB-N, TBA-RS, total aerobic bacteria count, psychrophilic count, molds and yeasts count. The sensory properties of all formulations prepared with bee pollen were acceptable. Bee pollen was superior as an antimicrobial and antioxidant agent for sausage. It could be concluded that bee pollen can be used by meat manufacturers for enhancing quality and extending the shelf-life.
天然添加剂可提高加工肉制品的微生物和氧化质量。蜂花粉是一种具有重要营养价值的天然成分。它被认为是一种天然抗菌剂和抗氧化剂。本研究的目的是在牛肉香肠配方中加入蜂花粉作为抗菌剂和抗氧化剂,以改善冷藏条件下的质量标准。研究人员制备了五种牛肉香肠配方:第一种配方是由含有丁基羟基甲苯(BHT)的基本配方(阳性对照)制备而成;第二种配方(阴性对照)与基本配方相同,不含 BHT;其他三种配方分别含有 1%、2% 和 3%的桉树(桉树属)蜂花粉。所有配方均储存在 5±2 °C,直至出现变质症状。对化学成分、硫代巴比妥酸活性物质(TBA-RS)、总挥发性碱基氮(TVB-N)、微生物数量和感官特性进行了评估。将所得结果与埃及标准进行比较,以评估其可接受性。与香肠基本配方相比,用 BHT 和蜂花粉处理过的配方在 TVB-N、TBA-RS、需氧细菌总数、嗜灵菌计数、霉菌和酵母菌计数方面均有显著降低。用蜂花粉制备的所有配方的感官特性均可接受。作为香肠的抗菌剂和抗氧化剂,蜂花粉更胜一筹。因此,肉类生产商可以使用蜂花粉来提高产品质量和延长保质期。
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引用次数: 0
Association Between Paclitaxel and Stroke: Mendelian Randomization 紫杉醇与中风的关系:孟德尔随机化
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2344
Bingwu Chen, Xian Zhou, Tao Yang, Mei-xia Xu, Tao Liu, Zhong Dai, Chuanhua Bi, Tao Xu
Paclitaxel, a naturally derived anticancer drug extracted from the plant Taxus brevifolia, holds significant medicinal and clinical value. Recently, more and more studies have shown that paclitaxel may be involved in the risk of stroke. However, there is still controversy over the relationship between the two at present. The objective of this study is to investigate a causality between paclitaxel and stroke using a two-sample Mendelian randomization (MR). Summary statistics for paclitaxel and stroke were derived from multiple comprehensive genome-wide association studies. The Inverse-variance weighting (IVW) method was used for preliminary analysis. Additionally, MR Steiger was employed to evaluate the robustness of these genetic variants in relation to paclitaxel and stroke. This study did not find a correlation between paclitaxel and stroke (OR: 1.008; 95% CI: 0.894–1.013, P = 0.270). This relationship was supported by other analysis methods as well. Furthermore, MR Steiger’s analysis confirmed a causal relationship between paclitaxel and stroke ( “TRUE”). Our research has shown that paclitaxel does not lead to stroke, and further exploration of the relationship between the two is still needed.
紫杉醇是从植物紫杉(Taxus brevifolia)中提取的天然抗癌药物,具有重要的药用和临床价值。最近,越来越多的研究表明,紫杉醇可能与中风风险有关。然而,目前对两者之间的关系仍存在争议。本研究的目的是采用双样本孟德尔随机法(MR)研究紫杉醇与中风之间的因果关系。紫杉醇与中风的汇总统计数据来自多项综合性全基因组关联研究。初步分析采用了逆方差加权法(IVW)。此外,还采用了 MR Steiger 来评估这些基因变异与紫杉醇和中风关系的稳健性。该研究未发现紫杉醇与中风之间存在相关性(OR:1.008;95% CI:0.894-1.013,P = 0.270)。其他分析方法也支持这种关系。此外,MR Steiger 的分析证实了紫杉醇与中风之间的因果关系("TRUE")。我们的研究表明,紫杉醇不会导致中风,因此仍需进一步探讨两者之间的关系。
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引用次数: 0
Analysis of Targeted Components in the Combination Extract of Hovenia dulcis and Phyllanthus emblica by UPLC-MS/MS 利用 UPLC-MS/MS 分析枳椇子和余甘子复合提取物中的目标成分
IF 0.5 4区 医学 Pub Date : 2024-01-01 DOI: 10.1166/jbmb.2024.2346
Mei Zhang, Hongyuan Zhang, Zhiwei Huang, Yanjie Zhang, Ling liang, Benyong Lou
This study aimed to establish a UPLC-MS/MS method for rapid analysis and identification of target components in the combination extract of Hovenia dulcis and Phyllanthus emblica. The gradient elution was carried out with methanol (A) −0.2% formic acid solution (A) with low rate of 1.0 mL · min−1, column temperature of 30 °C, detection wavelength of 285 nm. The ESI ion source was used to collect data under the negative ion mode. Through analysis and identification, five target components were inferred, namely gallic acid, dihydromyricetin, corilagine, taxifolin, and ellagic acid in the combination extract. Quantitative analysis was conducted on the combination extract. By utilizing the theory of modern supramolecular technology in traditional Chinese medicine, the functional composite product was obtained by selecting the compound extraction of Hovenia dulcis and Phyllanthus emblica as raw materials, and quantitative analysis was conducted on on the combination extract. The results showed that the flavonoid extraction rate and the content of target components were both higher when the mass ratio of Hovenia dulcis to Phyllanthus emblica was 1:1 and 1:2. This indicates that the co-extraction of Hovenia dulcis and Phyllanthus emblica not only simplified the extraction process but also enhances the extraction rate of the flavonoids in Hovenia dulcis. The developed UPLC-MS/MS method is rapid, sensitive, which provides a technical basis for the selection of raw material mass ratios and product development of Hovenia dulcis and Phyllanthus emblica combination.
本研究旨在建立一种UPLC-MS/MS方法,用于快速分析和鉴定枳椇子和白花蛇舌草提取物中的目标成分。采用甲醇(A)-0.2%甲酸溶液(A)进行梯度洗脱,流速为1.0 mL - min-1,柱温为30 ℃,检测波长为285 nm。在负离子模式下使用 ESI 离子源采集数据。通过分析和鉴定,推断出组合提取物中含有 5 种目标成分,即没食子酸、二氢杨梅素、柯里拉京、紫杉叶素和鞣花酸。对组合提取物进行了定量分析。利用现代中药超分子技术理论,选择枳壳和白术为原料进行复合提取,得到了功能性复合产品,并对复合提取物进行了定量分析。结果表明,当枳椇子与白花蛇舌草的质量比为 1:1 和 1:2 时,黄酮提取率和目标成分含量均较高。这表明枳椇子和白皮共萃取不仅简化了萃取过程,而且提高了枳椇子中黄酮类化合物的萃取率。所建立的UPLC-MS/MS方法快速、灵敏,为枳椇子和白皮的原料质量比选择和产品开发提供了技术依据。
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Journal of Biobased Materials and Bioenergy
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