The common ground sample method of vegetation survey requires a lot of manpower and time. Meanwhile, many vegetation information may not be revealed because the scale of standard sample is usually only 100 m2. For this reason, this study developed a set of desert vegetation sample survey method based on UAV technology, and further developed a set of vegetation spatial distribution pattern analysis method based on it, and carried out an empirical study with typical shrub communities in Inner Mongolia as an example. It is expected to provide scientific basis for wind and sand control, vegetation restoration and ecological construction in the ecologically fragile area at the edge of Inner Mongolia.
{"title":"The Classification Characteristics and Dynamic Changes of Desert Vegetation Based on Unmanned Aerial Vehicle Remote Sensing","authors":"Kun Yue, Penghui Li","doi":"10.1166/jbmb.2023.2316","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2316","url":null,"abstract":"The common ground sample method of vegetation survey requires a lot of manpower and time. Meanwhile, many vegetation information may not be revealed because the scale of standard sample is usually only 100 m2. For this reason, this study developed a set of desert vegetation sample survey method based on UAV technology, and further developed a set of vegetation spatial distribution pattern analysis method based on it, and carried out an empirical study with typical shrub communities in Inner Mongolia as an example. It is expected to provide scientific basis for wind and sand control, vegetation restoration and ecological construction in the ecologically fragile area at the edge of Inner Mongolia.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 4","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amit Kumar Maurya, Aditya, Vinny John, Hemlata Pant, Sujata P. Sharma, Doaa Z. El-Refaey, R. Sami, M. Helal, Fadi Baakdah, Nevin Ahmed
Pigeonpea (Cajanus cajan) holds significant economic importance as a versatile crop due to its various uses. It is a valuable source of nutrition for human consumption, rich in protein, dietary fiber, vitamins and minerals. Additionally, pigeonpea plays a crucial role in sustainable agriculture by fixing atmospheric nitrogen and enhancing soil fertility. Fusarium wilt caused by Fusarium udum is an important economic threat to the growers of pigeonpea in India and across the globe. The study was therefore conducted during the years 2017–2019 to determine the impact of the various oil seed cakes on the fusarium wilt of pigeonpea. Seven treatments including different types of oil seed cakes viz., mustard cake, neem cake, linseed cake, mahua cake, sunflower cake, groundnut cake and carbendazim (treated check) were employed by adopting the soil inoculation method. All of the treatments employed notably diminished the incidence of fusarium wilt. Among the treatments, neem cake (T2) was most effective in suppressing disease incidence (73.78% and 76.89%), registering the least plant fatalities (19.05 and 18.02), respectively. Furthermore, treatment (T2) also showed maximum plant height at 120 DAS (151.86 cm and 153.34 cm) and the highest root-nodules count of pigeon pea (5.71, 5.66) respectively in both the cropping years compared to other treatments and untreated control (T0).
鸽豆(Cajanus cajan)由于其多种用途,作为一种多功能作物具有重要的经济意义。它是人类食用的宝贵营养来源,富含蛋白质、膳食纤维、维生素和矿物质。此外,鸽子豆通过固定大气氮和提高土壤肥力在可持续农业中起着至关重要的作用。由镰刀菌引起的枯萎病是对印度乃至全球鸽豆种植者的重要经济威胁。因此,该研究在2017-2019年期间进行,以确定各种油籽饼对鸽子豌豆枯萎病的影响。采用土壤接种法对不同类型油籽饼、芥菜饼、楝树饼、亚麻籽饼、麻花饼、向日葵饼、花生饼和多菌灵(处理对照)进行处理。所有处理均显著降低了枯萎病的发病率。其中,印楝饼(T2)抑制病害发生率最高(73.78%和76.89%),植株死亡率最低(分别为19.05和18.02)。与其他处理和未处理对照(T0)相比,T2处理(T2)在两个种植年份的最大株高为120 DAS (151.86 cm和153.34 cm),根瘤数最高(5.71、5.66)。
{"title":"Unveiling Oil Seed Cakes Ability to Suppress Fusarium Wilt (Fusarium udum Butler) in Pigeonpea (Cajanus cajan L. Millsp.)","authors":"Amit Kumar Maurya, Aditya, Vinny John, Hemlata Pant, Sujata P. Sharma, Doaa Z. El-Refaey, R. Sami, M. Helal, Fadi Baakdah, Nevin Ahmed","doi":"10.1166/jbmb.2023.2319","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2319","url":null,"abstract":"Pigeonpea (Cajanus cajan) holds significant economic importance as a versatile crop due to its various uses. It is a valuable source of nutrition for human consumption, rich in protein, dietary fiber, vitamins and minerals. Additionally, pigeonpea plays a crucial role in sustainable\u0000 agriculture by fixing atmospheric nitrogen and enhancing soil fertility. Fusarium wilt caused by Fusarium udum is an important economic threat to the growers of pigeonpea in India and across the globe. The study was therefore conducted during the years 2017–2019 to determine the\u0000 impact of the various oil seed cakes on the fusarium wilt of pigeonpea. Seven treatments including different types of oil seed cakes viz., mustard cake, neem cake, linseed cake, mahua cake, sunflower cake, groundnut cake and carbendazim (treated check) were employed by adopting the\u0000 soil inoculation method. All of the treatments employed notably diminished the incidence of fusarium wilt. Among the treatments, neem cake (T2) was most effective in suppressing disease incidence (73.78% and 76.89%), registering the least plant fatalities (19.05 and 18.02), respectively.\u0000 Furthermore, treatment (T2) also showed maximum plant height at 120 DAS (151.86 cm and 153.34 cm) and the highest root-nodules count of pigeon pea (5.71, 5.66) respectively in both the cropping years compared to other treatments and untreated control (T0).","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 12","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138614781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Min Jiang, Yin Lin, Jinghua Li, Haoran Yang, Yingquan Fang
Euchresta japonica, Platycodon grandiflorum, Belamcanda chinensis, and Adenophora tetraphylla are the four medicinal substances that make up the Lianjie Liyan compound mixture, a type of compound preparation for treating throat diseases such as pharyngitis and laryngeal carcinoma. The preparation has been used clinically for a long time and has been proven that it has curative properties in terms of alleviating pain and other symptoms. However, no quality control research on this preparation has been done yet. The pharmacological components in the main quality control standards, including Oxymatrine, matrine, N-methylcytisine, platycoside-D in Platycodon grandiflorum, and irisaxanthin in Shegan, were analyzed to further standardize the clinically rational use of this preparation and prevent the occurrence of adverse reactions and side effects. In this study, the five active components in the mixture were determined concurrently for the first time with the help of Box-Behnken design and response surface approach. The properties of established model showed excellent resolution and sensitivity, good reproducibility and speed, and accurate prediction thanks to the optimization of HPLC measurement conditions. The established approach was precise and trustworthy, and it offered helpful support and references for the construction of the Lianjie Liyan compound mixture as well as for the quality control.
{"title":"Determination and Method Optimization of Lianjie Liyan Compound Mixture","authors":"Min Jiang, Yin Lin, Jinghua Li, Haoran Yang, Yingquan Fang","doi":"10.1166/jbmb.2023.2337","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2337","url":null,"abstract":"Euchresta japonica, Platycodon grandiflorum, Belamcanda chinensis, and Adenophora tetraphylla are the four medicinal substances that make up the Lianjie Liyan compound mixture, a type of compound preparation for treating throat diseases such as pharyngitis\u0000 and laryngeal carcinoma. The preparation has been used clinically for a long time and has been proven that it has curative properties in terms of alleviating pain and other symptoms. However, no quality control research on this preparation has been done yet. The pharmacological components\u0000 in the main quality control standards, including Oxymatrine, matrine, N-methylcytisine, platycoside-D in Platycodon grandiflorum, and irisaxanthin in Shegan, were analyzed to further standardize the clinically rational use of this preparation and prevent the occurrence of adverse reactions\u0000 and side effects. In this study, the five active components in the mixture were determined concurrently for the first time with the help of Box-Behnken design and response surface approach. The properties of established model showed excellent resolution and sensitivity, good reproducibility\u0000 and speed, and accurate prediction thanks to the optimization of HPLC measurement conditions. The established approach was precise and trustworthy, and it offered helpful support and references for the construction of the Lianjie Liyan compound mixture as well as for the quality control.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 10","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138614412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heavy metal pollution, especially arsenic (As) and antimony (Sb) pollution, seriously threatens people’s health and the ecological system. It is a common occurrence for As and Sb to coexist in the environment as inorganic species, including trivalent/pentavalent As or trivalent/pentavalent Sb. In addition, trivalent As and trivalent Sb are more toxic and difficult to remove than pentavalent As and pentavalent Sb. Therefore, the simultaneous pre-oxidation of trivalent As and trivalent Sb to pentavalent As and pentavalent Sb is a very critical step in the process of pollution control. A strain of Pseudomonas stutzeri, by which trivalent As/trivalent Sb can be oxidized to pentavalent As/pentavalent Sb, was selected, and its oxidation characteristics were studied in detail. The oxidation mechanism was thoroughly analyzed through a series of experiments and characterization. The results indicated that Pseudomonas stutzeri could tolerate trivalent As (577 mg/L) and trivalent Sb (1752 mg/L); the highest oxidation ratio of trivalent As was 53.05% and that of trivalent Sb was 83.05%. According to the cytotoxicity assay, the largest inhibition ratio was 89.22% for trivalent As and 74.98% for trivalent Sb under 5 mM of trivalent As/trivalent Sb stress. In addition, the minimum inhibitory concentrations of trivalent As and trivalent Sb were 0.22 mM and 0.43 mM, respectively. The results of Raman and FTIR spectroscopy analyses revealed that the peak vibration intensity of all strains under trivalent As and trivalent Sb stress was weaker than that of the control group. Therefore, it could be concluded that the toxicity of trivalent As for Pseudomonas stutzeri is greater than that of trivalent Sb according to the maximum concentration tolerated by Pseudomonas stutzeri and the minimum inhibitory concentrations. The oxidation ratio of trivalent Sb for Pseudomonas stutzeri is higher than that of trivalent As, with both trivalent As and trivalent Sb being bonded to the organic groups. The simultaneous oxidation of Sb(III) and As(III) by Pseudomonas stutzeri suggests its potential as a candidate for bioremediation of environments contaminated with these elements. This study provides a theoretical basis and guidance for the microbial oxidation of trivalent As and trivalent Sb.
{"title":"Characteristics of Pseudomonas stutzeri Oxidation of As(III)/Sb(III)","authors":"Miao Tian, Linfeng Jin, Zhouyou Xu, Erke Zhang, Biyu Li, Xinhui Deng, Liang Chen","doi":"10.1166/jbmb.2023.2331","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2331","url":null,"abstract":"Heavy metal pollution, especially arsenic (As) and antimony (Sb) pollution, seriously threatens people’s health and the ecological system. It is a common occurrence for As and Sb to coexist in the environment as inorganic species, including trivalent/pentavalent As or trivalent/pentavalent\u0000 Sb. In addition, trivalent As and trivalent Sb are more toxic and difficult to remove than pentavalent As and pentavalent Sb. Therefore, the simultaneous pre-oxidation of trivalent As and trivalent Sb to pentavalent As and pentavalent Sb is a very critical step in the process of pollution\u0000 control. A strain of Pseudomonas stutzeri, by which trivalent As/trivalent Sb can be oxidized to pentavalent As/pentavalent Sb, was selected, and its oxidation characteristics were studied in detail. The oxidation mechanism was thoroughly analyzed through a series of experiments and\u0000 characterization. The results indicated that Pseudomonas stutzeri could tolerate trivalent As (577 mg/L) and trivalent Sb (1752 mg/L); the highest oxidation ratio of trivalent As was 53.05% and that of trivalent Sb was 83.05%. According to the cytotoxicity assay, the largest inhibition\u0000 ratio was 89.22% for trivalent As and 74.98% for trivalent Sb under 5 mM of trivalent As/trivalent Sb stress. In addition, the minimum inhibitory concentrations of trivalent As and trivalent Sb were 0.22 mM and 0.43 mM, respectively. The results of Raman and FTIR spectroscopy analyses revealed\u0000 that the peak vibration intensity of all strains under trivalent As and trivalent Sb stress was weaker than that of the control group. Therefore, it could be concluded that the toxicity of trivalent As for Pseudomonas stutzeri is greater than that of trivalent Sb according to the maximum\u0000 concentration tolerated by Pseudomonas stutzeri and the minimum inhibitory concentrations. The oxidation ratio of trivalent Sb for Pseudomonas stutzeri is higher than that of trivalent As, with both trivalent As and trivalent Sb being bonded to the organic groups. The simultaneous\u0000 oxidation of Sb(III) and As(III) by Pseudomonas stutzeri suggests its potential as a candidate for bioremediation of environments contaminated with these elements. This study provides a theoretical basis and guidance for the microbial oxidation of trivalent As and trivalent Sb.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 17","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138618536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As an important economic aquatic animal, red crayfish’s reproduction and incubation can affect its breeding efficiency. With aquaculture developing, the study of factors affecting aquaculture species growth has gradually become an important research direction. To study the effects of ammonia nitrogen, nitrite and pH on red crayfish embryos, the In Vitro incubation experiment of red crayfish embryos was conducted. The residual module was used to design the embryo counting method of red crayfish. And different ammonia nitrogen, nitrite and PH gradients were set to analyze the hatching rate and emergence rate of red crayfish’s embryo development.When conducting research method performance testing, the training loss value of the research method decreased to 84 after 200 iterations. On the 6th day in an ammonia nitrogen environment, the number of water microbiota was only 4.74 M. The emergence rate of red crayfish decreases to a lower level when the ammonia nitrogen concentration is above 0.6 mg/L. The emergence rate of red crayfish decreased with the increase of nitrite concentration, and decreased to 0 when the nitrite concentration reached 1.25 mg/L. When the pH value of water body is 8.1, the emergence rate of red crayfish reaches the highest value of 68.92%. Ammonia nitrogen and nitrite concentration will inhibit the In Vitro hatching of crayfish embryos, and the appropriate PH value can improve the emergence rate of crayfish hatching In Vitro.
{"title":"Effects of Ammonia Nitrogen, Nitrite and pH on Embryo In Vitro Incubation of Red Crayfish","authors":"Jianguo Wang, Gang Li, Yongjun Bao","doi":"10.1166/jbmb.2023.2324","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2324","url":null,"abstract":"As an important economic aquatic animal, red crayfish’s reproduction and incubation can affect its breeding efficiency. With aquaculture developing, the study of factors affecting aquaculture species growth has gradually become an important research direction. To study the effects\u0000 of ammonia nitrogen, nitrite and pH on red crayfish embryos, the In Vitro incubation experiment of red crayfish embryos was conducted. The residual module was used to design the embryo counting method of red crayfish. And different ammonia nitrogen, nitrite and PH gradients were set\u0000 to analyze the hatching rate and emergence rate of red crayfish’s embryo development.When conducting research method performance testing, the training loss value of the research method decreased to 84 after 200 iterations. On the 6th day in an ammonia nitrogen environment, the number\u0000 of water microbiota was only 4.74 M. The emergence rate of red crayfish decreases to a lower level when the ammonia nitrogen concentration is above 0.6 mg/L. The emergence rate of red crayfish decreased with the increase of nitrite concentration, and decreased to 0 when the nitrite concentration\u0000 reached 1.25 mg/L. When the pH value of water body is 8.1, the emergence rate of red crayfish reaches the highest value of 68.92%. Ammonia nitrogen and nitrite concentration will inhibit the In Vitro hatching of crayfish embryos, and the appropriate PH value can improve the emergence\u0000 rate of crayfish hatching In Vitro.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 549","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to explore the quality differences of various brands of low-temperature yogurt sold in the market, the nutritional components and physical characteristics of 11 kinds of stirred lowtemperature commercial yogurt were analyzed by principal component analysis (PCA), including protein content, total sugar content, fat content, sodium content, calcium content, probiotic content, solid content, pH value, L*, a*, b*, viscosity and whey precipitation rate. In addition, combined with the method of sensory evaluation, the quality evaluation model is initially constructed. The results show that the cumulative variance contribution rate of the four principal components extracted by principal component analysis method reaches 90.74%. Based on the comprehensive evaluation function of principal component analysis method, the calculated results are significantly positively correlated with the sensory evaluation results (r = 0.909, P <0.001), which reflects the high reliability and effectiveness of the evaluation model. By cluster analysis, 11 kinds of yogurt can be divided into four groups. The first group is characterized by high protein and mellow taste, the second group is characterized by balanced nutrition and popular taste, the third group is characterized by no additive and original flavor, and the fourth group is characterized by moderate quality in all aspects. The research results provide a theoretical basis for the quality evaluation of stirred low-temperature yogurt and the research and development of fermented dairy products.
为了探究市场上销售的不同品牌低温酸奶的品质差异,采用主成分分析法(PCA)对11种搅拌低温商品酸奶的营养成分和物理特性进行了分析,包括蛋白质含量、总糖含量、脂肪含量、钠含量、钙含量、益生菌含量、固体含量、pH值、L*、a*、b*、粘度和乳清沉淀率。并结合感官评价方法,初步构建了质量评价模型。结果表明,主成分分析法提取的4个主成分的累积方差贡献率达到90.74%。基于主成分分析法的综合评价函数,计算结果与感官评价结果显著正相关(r = 0.909, P <0.001),反映了评价模型的高可靠性和有效性。通过聚类分析,将11种酸奶分为4类。第一组以高蛋白、口感醇厚为特点,第二组以营养均衡、口味通俗为特点,第三组以无添加剂、原味为特点,第四组以各方面品质适中为特点。研究结果为搅拌低温酸奶的质量评价和发酵乳制品的研究开发提供了理论依据。
{"title":"Nutritional and Sensory Quality Evaluation of Low-Temperature Yogurt Sold in the Market Based on Principal Component Analysis and Cluster Analysis","authors":"Siran Zhang","doi":"10.1166/jbmb.2023.2317","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2317","url":null,"abstract":"In order to explore the quality differences of various brands of low-temperature yogurt sold in the market, the nutritional components and physical characteristics of 11 kinds of stirred lowtemperature commercial yogurt were analyzed by principal component analysis (PCA), including\u0000 protein content, total sugar content, fat content, sodium content, calcium content, probiotic content, solid content, pH value, L*, a*, b*, viscosity and whey precipitation rate. In addition, combined with the method of sensory evaluation, the quality evaluation model\u0000 is initially constructed. The results show that the cumulative variance contribution rate of the four principal components extracted by principal component analysis method reaches 90.74%. Based on the comprehensive evaluation function of principal component analysis method, the calculated\u0000 results are significantly positively correlated with the sensory evaluation results (r = 0.909, P <0.001), which reflects the high reliability and effectiveness of the evaluation model. By cluster analysis, 11 kinds of yogurt can be divided into four groups. The first group\u0000 is characterized by high protein and mellow taste, the second group is characterized by balanced nutrition and popular taste, the third group is characterized by no additive and original flavor, and the fourth group is characterized by moderate quality in all aspects. The research results\u0000 provide a theoretical basis for the quality evaluation of stirred low-temperature yogurt and the research and development of fermented dairy products.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angelica sinensis (A. sinensis), a traditional Chinese medicine (TCM), can tonify the blood, promote circulation, and nourish the body. This study investigates the potential of A. sinensis extract in improving ovarian function at the molecular level and its combined effects with other natural medicinal herbs in patients undergoing myomectomy. The impact of A. sinensis extract was assessed using a rat model of premature ovarian insufficiency. The rats were divided into four groups: control group, model group, low-dose Angelica group, and high-dose Angelica group. Serum levels of estradiol (E2), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were determined, and the pathology of ovarian tissue was examined. The results revealed elevated expressions of E2, nuclear factor erythroid 2-related factor 2 (Nrf-2), and heme oxygenase-1 (HO-1) in the treated groups compared to the model group, along with decreased levels of LH, FSH, interleukin (IL)-4, IL-6, and tumor necrosis factor-α. In a clinical trial involving patients after myomectomy, the efficacy of a combination of TCM decoction containing A. sinensis and enhanced recovery after surgery nursing model was evaluated with anxiety, depression, and ovarian function as indicators. The observation group exhibited reduced levels of anxiety and depression, along with improved ovarian function compared to the control group. Moreover, it was observed that A. sinensis extract demonstrated the potential in enhancing ovarian function through molecular pathways, possibly involving Nrf-2/HO-1 signaling. These findings provide insights into novel therapeutic approaches for promoting ovarian health and emphasize the benefits of integrating traditional medicine with modern care strategies.
{"title":"Effect of a Self-Made Herbal Decoction Containing Angelica sinensis Combined with an Enhanced Recovery After Surgery Nursing Model on Anxiety, Depression, and Ovarian Function in Patients Undergoing Myomectomy","authors":"Xiaomei Dong, Yunjiao Zheng","doi":"10.1166/jbmb.2023.2332","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2332","url":null,"abstract":"Angelica sinensis (A. sinensis), a traditional Chinese medicine (TCM), can tonify the blood, promote circulation, and nourish the body. This study investigates the potential of A. sinensis extract in improving ovarian function at the molecular level and its combined\u0000 effects with other natural medicinal herbs in patients undergoing myomectomy. The impact of A. sinensis extract was assessed using a rat model of premature ovarian insufficiency. The rats were divided into four groups: control group, model group, low-dose Angelica group, and high-dose\u0000 Angelica group. Serum levels of estradiol (E2), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were determined, and the pathology of ovarian tissue was examined. The results revealed elevated expressions of E2, nuclear factor erythroid 2-related factor\u0000 2 (Nrf-2), and heme oxygenase-1 (HO-1) in the treated groups compared to the model group, along with decreased levels of LH, FSH, interleukin (IL)-4, IL-6, and tumor necrosis factor-α. In a clinical trial involving patients after myomectomy, the efficacy of a combination of TCM\u0000 decoction containing A. sinensis and enhanced recovery after surgery nursing model was evaluated with anxiety, depression, and ovarian function as indicators. The observation group exhibited reduced levels of anxiety and depression, along with improved ovarian function compared to the\u0000 control group. Moreover, it was observed that A. sinensis extract demonstrated the potential in enhancing ovarian function through molecular pathways, possibly involving Nrf-2/HO-1 signaling. These findings provide insights into novel therapeutic approaches for promoting ovarian health\u0000 and emphasize the benefits of integrating traditional medicine with modern care strategies.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 32","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138619447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In Traditional Chinese Medicine, Cervical Softening Decoction is derived from a modified “Wan Bing Hui Chun” formulation and consists of natural herbal ingredients such as Angelica sinensis, Ligusticum chuanxiong, Leonurus heterophyllus, and Cortex Daphnes. The decoction regulates Qi, promotes blood circulation, and facilitates fetus descent. Genkwanin is an active compound in L. heterophyllus and previous studies identified this compound in ethanolic extracts from rat uterine smooth muscle cell membrane fractions. To further understand the pharmacological activities of Cervical Softening Decoction, we examined genkwanin effects on isolated rat uterine contractions and protein expression in rat tissue with postpartum hemorrhage complicated by multiple organ dysfunction syndrome (MODS). We also explored the practical effects of Cervical Softening Decoction combined with a birthing ball approach on promoting natural delivery in primiparous women. First, an isolated rat uterine smooth muscle contraction model was generated and three groups established: oxytocin group (0.002 U/mL), conventional genkwanin dose group (3 μg/mL), and high genkwanin dose group (6.0 μg/mL). Changes in uterine smooth muscle contraction and relaxation amplitudes were recorded before and after administration. Second, 50 rats were used to establish the following groups: (1) control group, (2) postpartum hemorrhage with MODS model group (MODS group), and (3) a postpartum hemorrhage with MODS treated with genkwanin group (genkwanin group). Western blotting was used to detect and compare tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 protein expression levels in lung tissue from groups. Finally, 84 primiparous women admitted to our hospital between January 2020 and December 2021 were selected and divided into two groups based on their obstetric interventions. The control group (n = 42) received routine obstetric intervention, while the observation group (n = 42) received Cervical Softening Decoction and delivery ball interventions. Delivery conditions were compared across groups. In basic studies, genkwanin doses significantly increased contraction and relaxation values in isolated rat uterine smooth muscle (P <0.05). Moreover, the genkwanin contractile effects at conventional and high doses were comparable with oxytocin. TNF-α and IL-6 protein expression in MODS group lung tissue was significantly higher when compared with the control group, while expression in the genkwanin group was significantly reduced when compared with the MODS group (P <0.05). In clinical studies, the observation group showed significantly higher natural delivery rates and Labor Agentry Scale (LAS) scores when compared with the control group. In contrast, cesarean section rates, first and second stage labor duration, total labor duration, and Facial Pain Scale (FPS) scores were significantly lower in the observation group when compared with the control group (P <0.05). In basic studies,
{"title":"Practical Effect of Cervical Softening Decoction Combined with Delivery Ball Approach in Promoting Vaginal Delivery in Primiparous Women","authors":"Shanshan Sun, Zhen Ye, Wenjun Wang","doi":"10.1166/jbmb.2023.2326","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2326","url":null,"abstract":"In Traditional Chinese Medicine, Cervical Softening Decoction is derived from a modified “Wan Bing Hui Chun” formulation and consists of natural herbal ingredients such as Angelica sinensis, Ligusticum chuanxiong, Leonurus heterophyllus, and Cortex\u0000 Daphnes. The decoction regulates Qi, promotes blood circulation, and facilitates fetus descent. Genkwanin is an active compound in L. heterophyllus and previous studies identified this compound in ethanolic extracts from rat uterine smooth muscle cell membrane fractions. To further\u0000 understand the pharmacological activities of Cervical Softening Decoction, we examined genkwanin effects on isolated rat uterine contractions and protein expression in rat tissue with postpartum hemorrhage complicated by multiple organ dysfunction syndrome (MODS). We also explored the practical\u0000 effects of Cervical Softening Decoction combined with a birthing ball approach on promoting natural delivery in primiparous women. First, an isolated rat uterine smooth muscle contraction model was generated and three groups established: oxytocin group (0.002 U/mL), conventional genkwanin\u0000 dose group (3 μg/mL), and high genkwanin dose group (6.0 μg/mL). Changes in uterine smooth muscle contraction and relaxation amplitudes were recorded before and after administration. Second, 50 rats were used to establish the following groups: (1) control group, (2) postpartum\u0000 hemorrhage with MODS model group (MODS group), and (3) a postpartum hemorrhage with MODS treated with genkwanin group (genkwanin group). Western blotting was used to detect and compare tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 protein expression levels\u0000 in lung tissue from groups. Finally, 84 primiparous women admitted to our hospital between January 2020 and December 2021 were selected and divided into two groups based on their obstetric interventions. The control group (n = 42) received routine obstetric intervention, while the observation\u0000 group (n = 42) received Cervical Softening Decoction and delivery ball interventions. Delivery conditions were compared across groups. In basic studies, genkwanin doses significantly increased contraction and relaxation values in isolated rat uterine smooth muscle (P <0.05).\u0000 Moreover, the genkwanin contractile effects at conventional and high doses were comparable with oxytocin. TNF-α and IL-6 protein expression in MODS group lung tissue was significantly higher when compared with the control group, while expression in the genkwanin group was significantly\u0000 reduced when compared with the MODS group (P <0.05). In clinical studies, the observation group showed significantly higher natural delivery rates and Labor Agentry Scale (LAS) scores when compared with the control group. In contrast, cesarean section rates, first and second stage\u0000 labor duration, total labor duration, and Facial Pain Scale (FPS) scores were significantly lower in the observation group when compared with the control group (P <0.05). In basic studies, ","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 7","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138620095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibiotic residues have significantly effects on aerobic composting process. This study was targeted at antibiotic composite pollutants (e.g., sulfanilamides, tetracyclines, and quinolones) and probed into the effects of pollution levels on aerobic composting. With higher initial antibiotic concentrations, the NH4+ –N and NO3− –N concentrations at the end of composting were higher, but TN content decreased, which may be due to the inhibition effects of antibiotics on nitrogen-fixing bacteria and thus were unfavorable for nitrogen accumulation. Antibiotic pollution obviously delayed composting maturity, and the antibiotics at initial concentration above 306.12 mg/kg were significantly toxic to seeds. Analysis of structural changes in dissolved organic matter (DOM) showed the humification after treatments CK and AT100 mainly occurred at the high-temperature stage (day 2–14) and later lower-temperature maturity stage (day 21–36). Humification after treatment AT300 mainly happened at the high-temperature stage (day 2–14), and that in AT600 mainly occurred at the early temperature rise and high-temperature stage (day 2–7). This study comprehensively evaluated the effect of antibiotic composite pollution on the maturity of aerobic compost, and provided a theoretical basis for the treatment of antibiotic composite pollution materials in practical production.
{"title":"Changes in Nitrogen and Humus During Aerobic Composting Under Antibiotic Composite Pollution","authors":"Lulu Zhang, Yingying Sun, Qiao Zeng, Jing Wang, Yulu Wei","doi":"10.1166/jbmb.2023.2343","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2343","url":null,"abstract":"Antibiotic residues have significantly effects on aerobic composting process. This study was targeted at antibiotic composite pollutants (e.g., sulfanilamides, tetracyclines, and quinolones) and probed into the effects of pollution levels on aerobic composting. With higher initial antibiotic\u0000 concentrations, the NH4+ –N and NO3− –N concentrations at the end of composting were higher, but TN content decreased, which may be due to the inhibition effects of antibiotics on nitrogen-fixing bacteria and thus were unfavorable\u0000 for nitrogen accumulation. Antibiotic pollution obviously delayed composting maturity, and the antibiotics at initial concentration above 306.12 mg/kg were significantly toxic to seeds. Analysis of structural changes in dissolved organic matter (DOM) showed the humification after treatments\u0000 CK and AT100 mainly occurred at the high-temperature stage (day 2–14) and later lower-temperature maturity stage (day 21–36). Humification after treatment AT300 mainly happened at the high-temperature stage (day 2–14), and that in AT600 mainly occurred at the early temperature\u0000 rise and high-temperature stage (day 2–7). This study comprehensively evaluated the effect of antibiotic composite pollution on the maturity of aerobic compost, and provided a theoretical basis for the treatment of antibiotic composite pollution materials in practical production.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":"26 5","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138624372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bizhen Luo, Dong Li, Ke Hu, Min Chen, Xiaoqin Sun, Ruisen Lu, Shujing Wei
Dioscorea futschauensis Uline ex R. Knuth, a perennial climbing herb belonging to the Stenophora section of the genus Dioscorea L. (Dioscoreaceae), originates from Southeast China and is highly regarded for its medicinal properties. Despite its medicinal significance, the absence of genomic information has impeded the effective utilization and conservation of this species. Here, we conducted genome survey sequencing of D. futschauensis to unveil its genomic characteristics and identify species-specific genomic markers, including whole plastome sequence, plastome-divergent hotspots, single-copy nuclear genes and polymorphic nuclear SSRs. The genome of D. futschauensis was approximated to be 1392.76 Mb in length, accompanied by a substantial heterozygosity rate of 1.75% and a repeat content of 67.43%. Comparative plastome analysis illuminated the conserved genome structure and gene content within D. futschauensis, and revealed four highly divergent hotspots (atpF intron, psbA-matK, matK -rps16, and rrn16-trnI) that could serve as molecular markers for D. futschauensis and its closely related species. Furthermore, more than 200 single-copy nuclear genes and 78 polymorphic nuclear SSRs were identified within D. futschauensis. Overall, the comprehensive genomic information and abundant genetic resources provided herein will not only facilitate extended research in the realms of population genetics, phylogenetics and conservation biology of D. futschauensis, but also hold considerable value for its cultivation and molecular breeding applications.
山药(Dioscorea futschauensis Uline ex R. Knuth)是山药科山药属(Dioscorea L.)的一种多年生攀援草本植物,原产于中国东南部,因其独特的药用价值而备受重视。尽管其具有重要的药用价值,但基因组信息的缺乏阻碍了该物种的有效利用和保护。本研究通过对福山雪豹进行基因组调查测序,揭示福山雪豹基因组特征,鉴定种特异性基因组标记,包括全质体序列、质体分化热点、单拷贝核基因和多态核ssr。futschauensis基因组长度约为1392.76 Mb,杂合率为1.75%,重复数为67.43%。对比质组分析揭示了该物种保守的基因组结构和基因含量,揭示了4个高度分化的热点(atpF内含子、psbA-matK、matK -rps16和rrn16-trnI),可作为该物种及其近缘种的分子标记。此外,还鉴定出200多个单拷贝核基因和78个多态核ssr。综上所述,本文所提供的全面的基因组信息和丰富的遗传资源,不仅有利于在狐猴种群遗传学、系统遗传学和保护生物学等领域的深入研究,而且对狐猴的培育和分子育种应用具有重要价值。
{"title":"Genome Survey Sequencing of the Medical Plant Dioscorea futschauensis: Genomic Characteristics Estimation and Genomic Resources Development","authors":"Bizhen Luo, Dong Li, Ke Hu, Min Chen, Xiaoqin Sun, Ruisen Lu, Shujing Wei","doi":"10.1166/jbmb.2023.2351","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2351","url":null,"abstract":"Dioscorea futschauensis Uline ex R. Knuth, a perennial climbing herb belonging to the Stenophora section of the genus Dioscorea L. (Dioscoreaceae), originates from Southeast China and is highly regarded for its medicinal properties. Despite its medicinal significance,\u0000 the absence of genomic information has impeded the effective utilization and conservation of this species. Here, we conducted genome survey sequencing of D. futschauensis to unveil its genomic characteristics and identify species-specific genomic markers, including whole plastome sequence,\u0000 plastome-divergent hotspots, single-copy nuclear genes and polymorphic nuclear SSRs. The genome of D. futschauensis was approximated to be 1392.76 Mb in length, accompanied by a substantial heterozygosity rate of 1.75% and a repeat content of 67.43%. Comparative plastome analysis illuminated\u0000 the conserved genome structure and gene content within D. futschauensis, and revealed four highly divergent hotspots (atpF intron, psbA-matK, matK -rps16, and rrn16-trnI) that could serve as molecular markers for D. futschauensis and its closely related species.\u0000 Furthermore, more than 200 single-copy nuclear genes and 78 polymorphic nuclear SSRs were identified within D. futschauensis. Overall, the comprehensive genomic information and abundant genetic resources provided herein will not only facilitate extended research in the realms of population\u0000 genetics, phylogenetics and conservation biology of D. futschauensis, but also hold considerable value for its cultivation and molecular breeding applications.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" 41","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}