This study explored the response characteristics and adaptation mechanisms of walnut saplings under calcium and magnesium deficiency. Using walnut saplings as the research subjects, and three treatment groups were established for pot experiments: no deficiency (control, CK), calcium deficiency (–Ca), and magnesium deficiency (–Mg). The development, physiological attributes, and mineral composition of walnut seedlings were assessed, with an analysis focusing on how deficiencies in calcium and magnesium impact these elements. Compared to the findings in the CK group, walnut plant height, ground diameter, leaf area, root length, root area, number of root tips, and biomass were significantly decreased in the –Ca and –Mg groups. However, the proportions of the root surface area attributable to level I and II roots were significantly increased in the –Ca and –Mg groups, whereas that attributable to level V roots was significantly decreased. The root-to-shoot ratio was significantly increased in both groups. Peroxidase (POD), proline (Pro), and malondialdehyde (MDA) levels in walnuts significantly increased under calcium deficiency ( p <0.05), Moreover, when there is a deficiency in magnesium, there is a notable escalation in the activities of SOD, POD, and catalase, as well as a marked rise in the levels of Pro, MDA, and soluble sugars. Calcium and magnesium deficiency significantly altered mineral content of walnuts. Calcium deficiency inhibited the absorption of N, Ca, Fe, Zn, Cu, and Mn, but promoted that of P, K, and Mg. Magnesium deficiency inhibited the absorption of N, P, K, Mg, and Fe, whereas it has no effect on other minerals. Long-term calcium and magnesium deficiency significantly affected the growth of walnut saplings, with magnesium deficiency having strong effects. However, saplings adjusted to mineral deficiency by enhancing the absorption capacity of their roots. Simultaneously, They stimulated the antioxidant protection mechanisms, modulating the activity of enzymes that combat oxidation, and altering the levels of free Pro and soluble sugars within cells to eliminate free radicals generated due to stress. This, in turn, alleviates the harm caused by lipid peroxidation and bolsters resistance to adverse stress.
{"title":"The Effects of Calcium and Magnesium Deficiency on the Growth, Physiology, and Nutrient Uptake Characteristics of Walnut Trees","authors":"Yushu Wang, Huajun Yang, Liben Wei, Honghai Liang, Xiaohui Huang, Jiajia Tang, Dalan Feng, Xiuzhen Li","doi":"10.1166/jbmb.2023.2305","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2305","url":null,"abstract":"This study explored the response characteristics and adaptation mechanisms of walnut saplings under calcium and magnesium deficiency. Using walnut saplings as the research subjects, and three treatment groups were established for pot experiments: no deficiency (control, CK), calcium deficiency (–Ca), and magnesium deficiency (–Mg). The development, physiological attributes, and mineral composition of walnut seedlings were assessed, with an analysis focusing on how deficiencies in calcium and magnesium impact these elements. Compared to the findings in the CK group, walnut plant height, ground diameter, leaf area, root length, root area, number of root tips, and biomass were significantly decreased in the –Ca and –Mg groups. However, the proportions of the root surface area attributable to level I and II roots were significantly increased in the –Ca and –Mg groups, whereas that attributable to level V roots was significantly decreased. The root-to-shoot ratio was significantly increased in both groups. Peroxidase (POD), proline (Pro), and malondialdehyde (MDA) levels in walnuts significantly increased under calcium deficiency ( p <0.05), Moreover, when there is a deficiency in magnesium, there is a notable escalation in the activities of SOD, POD, and catalase, as well as a marked rise in the levels of Pro, MDA, and soluble sugars. Calcium and magnesium deficiency significantly altered mineral content of walnuts. Calcium deficiency inhibited the absorption of N, Ca, Fe, Zn, Cu, and Mn, but promoted that of P, K, and Mg. Magnesium deficiency inhibited the absorption of N, P, K, Mg, and Fe, whereas it has no effect on other minerals. Long-term calcium and magnesium deficiency significantly affected the growth of walnut saplings, with magnesium deficiency having strong effects. However, saplings adjusted to mineral deficiency by enhancing the absorption capacity of their roots. Simultaneously, They stimulated the antioxidant protection mechanisms, modulating the activity of enzymes that combat oxidation, and altering the levels of free Pro and soluble sugars within cells to eliminate free radicals generated due to stress. This, in turn, alleviates the harm caused by lipid peroxidation and bolsters resistance to adverse stress.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A sensitive quantification method for sufentanil in human milk was developed. Samples were prepared by liquid-phase extraction. Analytes were chromatographically separated. The mobile phase contained 10 mM ammonium formate (A) and methanol (B). The ratios of A to B were 50:50, 10:90, and 50:50 sequentially. For LC-MS-MS detection, transition of the protonated precursor ions to product ions (sufentanil: m/z 387.2→m/z 238.2; internal control fentanil: m/z 337.2→m/z 158.2) was monitored. The linear quantification range of sufentanil concentration was 3.2–400 pg ·mL−1. The correlation coefficient (r2) was 0.996. The % RSD was less than 6.0% for intraday and less than 8.68% for interday precision. By this method, sufentanil contents were determined to be very low in the postnatal maternal milk samples.
{"title":"Measurement of Sufentanil in Human Milk by Liquid Chromatography-Tandem Mass Spectrometry","authors":"Yangfa Huang, Lianming Liao, Manlin Zeng, Qijian Huang, J. Zeng, Liangcheng Zhang","doi":"10.1166/jbmb.2023.2287","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2287","url":null,"abstract":"A sensitive quantification method for sufentanil in human milk was developed. Samples were prepared by liquid-phase extraction. Analytes were chromatographically separated. The mobile phase contained 10 mM ammonium formate (A) and methanol (B). The ratios of A to B were 50:50, 10:90,\u0000 and 50:50 sequentially. For LC-MS-MS detection, transition of the protonated precursor ions to product ions (sufentanil: m/z 387.2→m/z 238.2; internal control fentanil: m/z 337.2→m/z 158.2) was monitored. The linear quantification range of sufentanil concentration was 3.2–400\u0000 pg ·mL−1. The correlation coefficient (r2) was 0.996. The % RSD was less than 6.0% for intraday and less than 8.68% for interday precision. By this method, sufentanil contents were determined to be very low in the postnatal maternal milk samples.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48039360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Medicinal solution steam washing is a common clinical treatment for the management of various skin diseases and wound infections. According to traditional Chinese medicine, the lack of healing of infected incisions is often attributed to excessive intraoperative blood loss, resulting in an imbalance of Qi and Blood, meridian obstruction, blood stasis, and the accumulation of dryness, heat, and turbid Qi at the affected area. Therefore, detoxification washing of the affected area with medicinal solutions promotes healing and is clinically recommended. Based on this premise, a self-prepared medicinal solution using cold-natured Chinese medicinal herbs, such as Huangbai, Kushen, and Pugongying, was used to promote the healing of postpartum perineal incisions in women. The aim of this study was to determine the effects of a self-prepared medicinal solution combined with psychological care on the healing of postpartum perineal incisions. Eighty parturient women who underwent spontaneous delivery with a mediolateral episiotomy at our hospital from August 2020 to August 2021 were enrolled in the study. The parturient women were divided into control and observation groups using a random number table, with 40 patients per group. The control group received conventional treatment and nursing intervention. The observation group received intervention with self-prepared medicinal liquid fumigation using Huangbai and Kushen, combined with psychological care. Pain perception, incision healing time, and the psychological status of the two groups were compared. After 3 days of nursing, the visual analog scale (VAS) scores in the observation group were significantly lower than the scores in the control group (P <0.05). The VAS scores in the observation group were also significantly lower than the scores in the control group after nursing (P <0.05). The healing time of the incision in the observation group was significantly shorter than the healing time in the control group (P <0.05). After nursing, the self-rating anxiety scores and self-rating depression scores in the observation group were significantly lower than the scores in the control group (P <0.05). Compared to routine treatment and nursing interventions, the combined application of self-prepared medicinal solution steam washing and psychological care promotes the healing of postpartum perineal incisions, alleviates pain in the incision area, and improves negative emotional states in parturient women.
{"title":"The Effects of A Self-Prepared Medicinal Solution of Huangbai and Kushen Combined with Psychological Care on Postpartum Perineal Incisions","authors":"Zhijuan Zhang, Junhong Cai, Juan Xie","doi":"10.1166/jbmb.2023.2301","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2301","url":null,"abstract":"Medicinal solution steam washing is a common clinical treatment for the management of various skin diseases and wound infections. According to traditional Chinese medicine, the lack of healing of infected incisions is often attributed to excessive intraoperative blood loss, resulting\u0000 in an imbalance of Qi and Blood, meridian obstruction, blood stasis, and the accumulation of dryness, heat, and turbid Qi at the affected area. Therefore, detoxification washing of the affected area with medicinal solutions promotes healing and is clinically recommended. Based on this premise,\u0000 a self-prepared medicinal solution using cold-natured Chinese medicinal herbs, such as Huangbai, Kushen, and Pugongying, was used to promote the healing of postpartum perineal incisions in women. The aim of this study was to determine the effects of a self-prepared medicinal solution combined\u0000 with psychological care on the healing of postpartum perineal incisions. Eighty parturient women who underwent spontaneous delivery with a mediolateral episiotomy at our hospital from August 2020 to August 2021 were enrolled in the study. The parturient women were divided into control and\u0000 observation groups using a random number table, with 40 patients per group. The control group received conventional treatment and nursing intervention. The observation group received intervention with self-prepared medicinal liquid fumigation using Huangbai and Kushen, combined with psychological\u0000 care. Pain perception, incision healing time, and the psychological status of the two groups were compared. After 3 days of nursing, the visual analog scale (VAS) scores in the observation group were significantly lower than the scores in the control group (P <0.05). The VAS scores\u0000 in the observation group were also significantly lower than the scores in the control group after nursing (P <0.05). The healing time of the incision in the observation group was significantly shorter than the healing time in the control group (P <0.05). After nursing,\u0000 the self-rating anxiety scores and self-rating depression scores in the observation group were significantly lower than the scores in the control group (P <0.05). Compared to routine treatment and nursing interventions, the combined application of self-prepared medicinal solution\u0000 steam washing and psychological care promotes the healing of postpartum perineal incisions, alleviates pain in the incision area, and improves negative emotional states in parturient women.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43636194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to determine the effects of natural herbal acupoint application on anxiety in long-term hemodialysis patients and analyze the corresponding nursing measures. Between March 2020 and January 2022, 150 long-term hemodialysis patients admitted to our hospital were surveyed for this study. A questionnaire survey was conducted to determine the anxiety status of the patients. Patients with anxiety were registered and randomly divided into a research group and a control group. The control group received traditional routine nursing care, while the research group received personalized interventional nursing care combined with natural herbal acupoint application. The comfort level, sleep quality, emotional management ability, and negative emotional status before and after the intervention were compared between the two groups. The 58 (38.66%) patients who experienced anxiety were divided into control and research groups, with 29 patients per group. No significant differences in baseline characteristics were detected between the two groups (P >0.05). The research group had significantly higher General Comfort Questionnaire (GCQ) scores and significantly lower Pittsburgh Sleep Quality Index (PSQI) scores compared with the scores in the control group (P <0.05 for both). The research group had higher scores in emotional processing, interpersonal relationships, and psychological activity management than the control group, indicating better emotional management ability (P <0.05). Moreover, the research group had significantly lower scores on the Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) than the control group (P <0.05). In this study, targeted interventions combining individualized nursing care and traditional Chinese medicine acupoint patching were administered to patients, which effectively promoted their physical and mental well-being, enhanced patient comfort, improved sleep quality, and effectively managed patient anxiety, as well as alleviated negative emotions. Therefore, natural herbal patches are worthy of promotion and application in clinical settings.
{"title":"Impact of Natural Chinese Medicine Acupoint Application on Anxiety in Long-Term Hemodialysis Patients and Analysis of the Corresponding Nursing Measures","authors":"Juan Liu, Xinlian Geng","doi":"10.1166/jbmb.2023.2304","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2304","url":null,"abstract":"This study aimed to determine the effects of natural herbal acupoint application on anxiety in long-term hemodialysis patients and analyze the corresponding nursing measures. Between March 2020 and January 2022, 150 long-term hemodialysis patients admitted to our hospital were surveyed\u0000 for this study. A questionnaire survey was conducted to determine the anxiety status of the patients. Patients with anxiety were registered and randomly divided into a research group and a control group. The control group received traditional routine nursing care, while the research group\u0000 received personalized interventional nursing care combined with natural herbal acupoint application. The comfort level, sleep quality, emotional management ability, and negative emotional status before and after the intervention were compared between the two groups. The 58 (38.66%) patients\u0000 who experienced anxiety were divided into control and research groups, with 29 patients per group. No significant differences in baseline characteristics were detected between the two groups (P >0.05). The research group had significantly higher General Comfort Questionnaire (GCQ)\u0000 scores and significantly lower Pittsburgh Sleep Quality Index (PSQI) scores compared with the scores in the control group (P <0.05 for both). The research group had higher scores in emotional processing, interpersonal relationships, and psychological activity management than the\u0000 control group, indicating better emotional management ability (P <0.05). Moreover, the research group had significantly lower scores on the Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) than the control group (P <0.05). In this study, targeted interventions\u0000 combining individualized nursing care and traditional Chinese medicine acupoint patching were administered to patients, which effectively promoted their physical and mental well-being, enhanced patient comfort, improved sleep quality, and effectively managed patient anxiety, as well as alleviated\u0000 negative emotions. Therefore, natural herbal patches are worthy of promotion and application in clinical settings.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47106637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Archaeal communities from high temperature Daqu (HTD) and stillage are very vital for the production of Maotai-flavor liquor, for instance Danquan liquor. As an agent for saccharification and fermentation, Daqu severely influences the flavour of liquor. But archaeal compositions in three samples derived from Danquan company (dqjq_ck/dqjqcp/dqjp3) are indistinct at present, thereby hindering further improvement of Danquan liquor. The “dqjq_ck”/“dqjqcp” represent HTD at 45 d/135 d. The “dqjp3” represents stillage. In this paper, the archaeal compositions in three samples were demonstrated by amplicon sequencing. The archaeal compositions in all samples mainly included methanogenic archaea. The most abundant archaeal species were unclassified_Thaumarchaeota, Methanobrevibacter and Methanothrix in dqjq_ck/dqjqcp/dqjp3. The main archaea included Methanothrix, Methanobrevibacter, Methanobacterium, Nitrososphaera, Methanoregula, unclassified_Thaumarchaeota and unclassified_Methanoregulaceae in three samples. The archaeal succession (changes in the composition of archaea) in three sample types was clarified. This study will lay base for screening some excellent indigenous archaea and constructing its genetic engineering microbes to enhance the character of Danquan liquor.
{"title":"Analysis of Archaeal Diversity in Three Samples from Danquan Distillery","authors":"Shanling Gan, Qiman Zou, Yuwen Guo, Yujia Li, Donglin Yang, C. Shang","doi":"10.1166/jbmb.2023.2300","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2300","url":null,"abstract":"Archaeal communities from high temperature Daqu (HTD) and stillage are very vital for the production of Maotai-flavor liquor, for instance Danquan liquor. As an agent for saccharification and fermentation, Daqu severely influences the flavour of liquor. But archaeal compositions in\u0000 three samples derived from Danquan company (dqjq_ck/dqjqcp/dqjp3) are indistinct at present, thereby hindering further improvement of Danquan liquor. The “dqjq_ck”/“dqjqcp” represent HTD at 45 d/135 d. The “dqjp3” represents stillage. In this paper, the\u0000 archaeal compositions in three samples were demonstrated by amplicon sequencing. The archaeal compositions in all samples mainly included methanogenic archaea. The most abundant archaeal species were unclassified_Thaumarchaeota, Methanobrevibacter and Methanothrix in dqjq_ck/dqjqcp/dqjp3.\u0000 The main archaea included Methanothrix, Methanobrevibacter, Methanobacterium, Nitrososphaera, Methanoregula, unclassified_Thaumarchaeota and unclassified_Methanoregulaceae in three samples. The archaeal succession (changes in the composition of archaea) in three sample types was clarified.\u0000 This study will lay base for screening some excellent indigenous archaea and constructing its genetic engineering microbes to enhance the character of Danquan liquor.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47869597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the continued development of the modern economy, China has made great achievements in urbanisation. However, while China’s economic progress has been effective, it has also advocated the construction of green cities and the application of green materials in modern garden construction to build a livable life for people. In order to promote the use of natural green materials, materials should be planned well in modern garden construction. On the basis of making full use of these green materials, garden design is optimised with the help of natural green materials. This study analyses the current situation of the development of the urbanised garden industry, makes good site planning and design of modern gardens, applies natural green plants in urbanised construction, and increases the application rate of green, low-carbon and environmentally friendly materials in modern garden construction. The study provides a reference for the full use of green, low-carbon and environmentally friendly materials in modern garden construction to enhance the level of garden design.
{"title":"A Study on the Value of Green, Low Carbon and Environmentally Friendly Materials in Modern Gardens","authors":"Cai Wen","doi":"10.1166/jbmb.2023.2293","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2293","url":null,"abstract":"With the continued development of the modern economy, China has made great achievements in urbanisation. However, while China’s economic progress has been effective, it has also advocated the construction of green cities and the application of green materials in modern garden\u0000 construction to build a livable life for people. In order to promote the use of natural green materials, materials should be planned well in modern garden construction. On the basis of making full use of these green materials, garden design is optimised with the help of natural green materials.\u0000 This study analyses the current situation of the development of the urbanised garden industry, makes good site planning and design of modern gardens, applies natural green plants in urbanised construction, and increases the application rate of green, low-carbon and environmentally friendly\u0000 materials in modern garden construction. The study provides a reference for the full use of green, low-carbon and environmentally friendly materials in modern garden construction to enhance the level of garden design.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48701042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bensaad, H. Banjer, K. Alzahrani, M. Helal, R. Sami, A. Ashour, Naseh A. Algehainy, Suzan Harara
The scientific community has been mobilized in recent years at the international level to find concrete solution, in order to elaborate vaccines against Covid-19 with less side-effects and thus to overcome this pandemic situation. However, despite the fact that the actual situation is partially under control, the issue of variants remains very problematic until this day. Bioinformatics as an emerging discipline has allowed scientists to understand the pathophysiological mechanism of COVID-19, a disease characterized by its complexity, as well as the comprehension of other serious infectious diseases. In this context, this modest work aimed to explore for the first time the possible binding process of four phyto-compounds against Angiotensin Converting Enzyme 2 (ACE2) receptor using molecular docking approach. The molecular docking analysis suggested that our compounds may possibly interact with ACE2 receptor principally via hydrogen bound and hydrophobic contact, but with an antagonistic way. Data of this study also suggested that compounds 2 and 3 have the best and an exceptional equal binding energy score (−6.8 kcal/mol), among the four tested compounds. This research could be considered a primordial and initial step for future preclinical and clinical experiments investigating SARS-CoV-2 variants, especially to better understand the mechanism of action of ACE2 receptor and the mode of contamination of host cells by this virus.
{"title":"Advanced Molecular Docking Approach to Simulate Intermolecular Interaction and Binding Affinity Energy of Four Key Bio-Compounds Against Angiotensin Converting Enzyme 2 (ACE2) Receptor","authors":"M. Bensaad, H. Banjer, K. Alzahrani, M. Helal, R. Sami, A. Ashour, Naseh A. Algehainy, Suzan Harara","doi":"10.1166/jbmb.2023.2295","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2295","url":null,"abstract":"The scientific community has been mobilized in recent years at the international level to find concrete solution, in order to elaborate vaccines against Covid-19 with less side-effects and thus to overcome this pandemic situation. However, despite the fact that the actual situation\u0000 is partially under control, the issue of variants remains very problematic until this day. Bioinformatics as an emerging discipline has allowed scientists to understand the pathophysiological mechanism of COVID-19, a disease characterized by its complexity, as well as the comprehension of\u0000 other serious infectious diseases. In this context, this modest work aimed to explore for the first time the possible binding process of four phyto-compounds against Angiotensin Converting Enzyme 2 (ACE2) receptor using molecular docking approach. The molecular docking analysis suggested that\u0000 our compounds may possibly interact with ACE2 receptor principally via hydrogen bound and hydrophobic contact, but with an antagonistic way. Data of this study also suggested that compounds 2 and 3 have the best and an exceptional equal binding energy score (−6.8 kcal/mol), among the\u0000 four tested compounds. This research could be considered a primordial and initial step for future preclinical and clinical experiments investigating SARS-CoV-2 variants, especially to better understand the mechanism of action of ACE2 receptor and the mode of contamination of host cells by\u0000 this virus.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44567168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we first investigated the effects of different concentrations of NaCl stress treatments (0%, 0.1%, 0.2%, 0.3%, and 0.4%) on the anatomical structure and ultrastructure of plant leaves using annual seedlings of Berberis thunbergii var. atropurpurea and Berberis thunbergii ‘bingchuanhongye.’ To provide a detailed analysis, scanning and transmission electron microscopy was utilized. Scanning electron microscopy indicated that the increased NaCl stress concentration decreased the number of stomatal openings, open stomatal percentage, and stomatal length, width, and size of the lower epidermis of both Berberis species. The epidermal cells and stomata of Berberis thunbergii ‘bingchuanhongye’ were significantly wrinkled and damaged, while the arrangement of the palisade tissue and spongy tissue of Berberis thunbergii var. atropurpurea was more orderly than that of Berberis thunbergii ‘bingchuanhongye.’ Transmission electron microscopy showed that the cytoplasm of Berberis thunbergii var. atropurpurea gradually shrank and exhibited serious plasmolysis with the increase of NaCl stress concentration. At the same time, the chloroplasts became larger and deformed, and the number of mitochondria, osmiophilic granules, and starch granules gradually increased. In contrast, the inward contraction of the protoplasm of Berberis thunbergii ‘bingchuanhongye’ was not obvious, the chloroplasts were smaller and fewer in number, and the number of mitochondria, osmiophilic granules, and starch granules gradually decreased until degradation. This study not only preliminarily determined that Berberis thunbergii var. atropurpurea showed slightly higher salt tolerance than Berberis thunbergii ‘bingchuanhongye,’ but also provided insights into their response mechanisms to saline environments.
{"title":"Effect of NaCl Stress on the Anatomical Structure and Ultrastructure of Leaves of Berberis thunbergii var. atropurpurea and Berberis thunbergii ‘bingchuanhongye’","authors":"Yan Li, B. Lu, Yufei Zhu, Bing-she Lu","doi":"10.1166/jbmb.2023.2290","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2290","url":null,"abstract":"In this study, we first investigated the effects of different concentrations of NaCl stress treatments (0%, 0.1%, 0.2%, 0.3%, and 0.4%) on the anatomical structure and ultrastructure of plant leaves using annual seedlings of Berberis thunbergii var. atropurpurea and Berberis\u0000 thunbergii ‘bingchuanhongye.’ To provide a detailed analysis, scanning and transmission electron microscopy was utilized. Scanning electron microscopy indicated that the increased NaCl stress concentration decreased the number of stomatal openings, open stomatal percentage,\u0000 and stomatal length, width, and size of the lower epidermis of both Berberis species. The epidermal cells and stomata of Berberis thunbergii ‘bingchuanhongye’ were significantly wrinkled and damaged, while the arrangement of the palisade tissue and spongy tissue of Berberis\u0000 thunbergii var. atropurpurea was more orderly than that of Berberis thunbergii ‘bingchuanhongye.’ Transmission electron microscopy showed that the cytoplasm of Berberis thunbergii var. atropurpurea gradually shrank and exhibited serious plasmolysis\u0000 with the increase of NaCl stress concentration. At the same time, the chloroplasts became larger and deformed, and the number of mitochondria, osmiophilic granules, and starch granules gradually increased. In contrast, the inward contraction of the protoplasm of Berberis thunbergii\u0000 ‘bingchuanhongye’ was not obvious, the chloroplasts were smaller and fewer in number, and the number of mitochondria, osmiophilic granules, and starch granules gradually decreased until degradation. This study not only preliminarily determined that Berberis thunbergii var.\u0000 atropurpurea showed slightly higher salt tolerance than Berberis thunbergii ‘bingchuanhongye,’ but also provided insights into their response mechanisms to saline environments.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42774977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study analyzed the clinical effects of cervical dilation balloon and castor oil meal nursing interventions in term induction of labor. The eighty pregnant women who were scheduled for delivery in our hospital between March 2020 and January 2022 were recruited into an observation group and a control group using a random number table. The control group received oxytocin for labor induction while the observation group received cervical dilation balloon and castor oil meal nursing interventions for labor induction. In addition to clinical treatment experiments, cell experiments are performed. BeWo cells were cultured to study the effect of estrogen on cell viability, and the expression of matrix metalloproteinase 2 and 9 (MMP2, MMP9) was detected using Western blotting. The endpoints of this study were to investigate the impact of estrogen changes on term-induced pregnant women and to compare the cervical dilation balloon and castor oil meal nursing interventions with regards to duration of labor, efficacy of cervical ripening, Bishop score of the cervix, postpartum complications, and other relevant indicators. The viability of BeWo cells did not significantly differ after treatment with different concentrations of 17β-E2 (-> 0.05). The expression of MMP9 after different levels of treatment did not significantly differ (P >0.05), whereas the expression of MMP2 protein in the 5×10−7 and 5×10−8 M treatment groups was lower than that in the control group (P <0.05). The first stage and total duration of labor were significantly shorter in the observation group than that in the control group (P <0.05). The efficacy of cervical ripening was significantly higher in the observation group than that in the control group (P <0.05). The Bishop score of the cervix was significantly higher in the observation group after intervention (P <0.05). The incidence of complications in the observation group was significantly lower than that in the control group (P <0.05). Estrogen levels may affect the invasive function of trophoblast cells, causing abnormal placental function. Castor oil has hormonal effects and contains precursors for prostaglandin synthesis and can induce and strengthen contractions, soften the cervix, and dilate the cervix. The combined use of cervical dilation balloon and castor oil meal in term induction of labor has clear clinical effects, including the effective shortening of labor duration, favorable cervical ripening, significant improvement in the Bishop score of the cervix, and reduced incidence of complications. This study suggests its potential for clinical application and wider use.
{"title":"Clinical Effects of Cervical Dilation Balloon and Castor Oil Meal Nursing Interventions in Term Induction of Labor","authors":"Juan Xie, Zhijuan Zhang, Xiaofeng Xu","doi":"10.1166/jbmb.2023.2318","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2318","url":null,"abstract":"This study analyzed the clinical effects of cervical dilation balloon and castor oil meal nursing interventions in term induction of labor. The eighty pregnant women who were scheduled for delivery in our hospital between March 2020 and January 2022 were recruited into an observation\u0000 group and a control group using a random number table. The control group received oxytocin for labor induction while the observation group received cervical dilation balloon and castor oil meal nursing interventions for labor induction. In addition to clinical treatment experiments, cell experiments\u0000 are performed. BeWo cells were cultured to study the effect of estrogen on cell viability, and the expression of matrix metalloproteinase 2 and 9 (MMP2, MMP9) was detected using Western blotting. The endpoints of this study were to investigate the impact of estrogen changes on term-induced\u0000 pregnant women and to compare the cervical dilation balloon and castor oil meal nursing interventions with regards to duration of labor, efficacy of cervical ripening, Bishop score of the cervix, postpartum complications, and other relevant indicators. The viability of BeWo cells did not significantly\u0000 differ after treatment with different concentrations of 17β-E2 (-> 0.05). The expression of MMP9 after different levels of treatment did not significantly differ (P >0.05), whereas the expression of MMP2 protein in the 5×10−7 and 5×10−8\u0000 M treatment groups was lower than that in the control group (P <0.05). The first stage and total duration of labor were significantly shorter in the observation group than that in the control group (P <0.05). The efficacy of cervical ripening was significantly higher in\u0000 the observation group than that in the control group (P <0.05). The Bishop score of the cervix was significantly higher in the observation group after intervention (P <0.05). The incidence of complications in the observation group was significantly lower than that in the\u0000 control group (P <0.05). Estrogen levels may affect the invasive function of trophoblast cells, causing abnormal placental function. Castor oil has hormonal effects and contains precursors for prostaglandin synthesis and can induce and strengthen contractions, soften the cervix,\u0000 and dilate the cervix. The combined use of cervical dilation balloon and castor oil meal in term induction of labor has clear clinical effects, including the effective shortening of labor duration, favorable cervical ripening, significant improvement in the Bishop score of the cervix, and\u0000 reduced incidence of complications. This study suggests its potential for clinical application and wider use.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46363340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Binbin Qiao, Zhenghua Leng, Shixiang Mao, Qiang Wang, Han Liu
Soil and water resource mismanagement can negatively intensify global poverty and jeopardize ecology. Soil can be contaminated by heavy metals, organic chemicals like biological pathogens, pesticides, micro plastics and nano particles. Contamination decreases the soil’s capability to yield food thereby affecting food production by means of pollution and disease. Moreover, soil contaminants move into rivers damaging the water quality. Numerous technologies have been established to tackle water and soil remediation, such as deep excavation technique where transportation of contaminated soils or water is done to remote unpopulated sites. Recent development in Remote Sensing and geographic information processing techniques have led to exciting new opportunities for investigating and closely monitoring environmental factors that influence key land and soil management approaches. Hence, this paper uses the Deep Excavation and Remote Sensing based Assessment Model (DERSAM) to assess the soil and water pollution in contaminated areas. The data are taken from the Europe soil contamination map to classify the contaminated and non-contaminated areas. This data obtained from the high-resolution satellite of Remote Sensing (RS) technique and Geographical Information System (GIS) with the parallel expansion of a fully assimilated geospatial database system that provides monitoring and feedback at suitable spatial scales. Thus, such data can be utilized for long-term environmental management and monitoring of remediation and rehabilitation of excavation areas. The numerical outcomes show that the recommended DERSAM model increases the land use and land cover change prediction by 90.2%, the classification ratio by 98.2%, the pollution reduction ratio by 96.6%, the soil contamination detection ratio by 95.9%, and the overall performance by 97.2% compared to other existing approaches.
{"title":"Remote Sensing-Based Assessment of Soil and Water Pollution in Deep Excavation Scenario","authors":"Binbin Qiao, Zhenghua Leng, Shixiang Mao, Qiang Wang, Han Liu","doi":"10.1166/jbmb.2023.2289","DOIUrl":"https://doi.org/10.1166/jbmb.2023.2289","url":null,"abstract":"Soil and water resource mismanagement can negatively intensify global poverty and jeopardize ecology. Soil can be contaminated by heavy metals, organic chemicals like biological pathogens, pesticides, micro plastics and nano particles. Contamination decreases the soil’s capability\u0000 to yield food thereby affecting food production by means of pollution and disease. Moreover, soil contaminants move into rivers damaging the water quality. Numerous technologies have been established to tackle water and soil remediation, such as deep excavation technique where transportation\u0000 of contaminated soils or water is done to remote unpopulated sites. Recent development in Remote Sensing and geographic information processing techniques have led to exciting new opportunities for investigating and closely monitoring environmental factors that influence key land and soil management\u0000 approaches. Hence, this paper uses the Deep Excavation and Remote Sensing based Assessment Model (DERSAM) to assess the soil and water pollution in contaminated areas. The data are taken from the Europe soil contamination map to classify the contaminated and non-contaminated areas. This data\u0000 obtained from the high-resolution satellite of Remote Sensing (RS) technique and Geographical Information System (GIS) with the parallel expansion of a fully assimilated geospatial database system that provides monitoring and feedback at suitable spatial scales. Thus, such data can be utilized\u0000 for long-term environmental management and monitoring of remediation and rehabilitation of excavation areas. The numerical outcomes show that the recommended DERSAM model increases the land use and land cover change prediction by 90.2%, the classification ratio by 98.2%, the pollution reduction\u0000 ratio by 96.6%, the soil contamination detection ratio by 95.9%, and the overall performance by 97.2% compared to other existing approaches.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41391888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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