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An Insight-Based Computational Approaches to Estimate Molecular Weight Distribution, Allergenicity and Immunological Aspects, Toxicity Profile, Possible Biodegradation, Persistence and Bioaccumulation Factor of Four Phyto-Compounds 一种基于洞察力的计算方法来估计四种植物化合物的分子量分布、致敏性和免疫学方面、毒性特征、可能的生物降解性、持久性和生物累积因子
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2291
M. Bensaad, M. Kahoul, Mokrane Khier, Debasis Mitra, Mohammed Benhoula, H. Banjer, Rasha A. Al-Eisa, Naseh A. Algehainy, M. Helal, Amina A. M. Al-Mushhin, R. Sami
Clinical pharmacology and toxicology are considered nowadays two complementary and indispensable medical disciplines that allowed clinicians to achieve the optimal therapeutic effect of a several substance, but also to characterize their toxicity threshold. The contribution of bioinformatics tools and databases in these two fields has provided key elements, sometimes impossible to explain during early clinical phases. In this context, some computational approaches were used in this work to investigate the molecular weight distribution (MWD), allergenicity, toxicity profile, possible biodegradation, persistence and bioaccumulation factor of four natural compounds. Results revealed that MWD indices of compound 2 and 3 were the best among the tested compounds. However, the route of administration of compound 1 could be considered the safest for rats, especially the oral, intravenous and subcutaneous ways, in which LD50 values were the best, comparing to the other compounds. The two allergenicity tests revealed that the four phyto-compounds could be considered non-allergen agents and may not present any drug toxicity risks. Concerning cytotoxicity, compound 4 exhibited the best cytotoxic effect among these compounds with a corresponding value of Pa = 0.782 against colon carcinoma (HCT-116) cell line. The four bio-compounds could be toxic for avian species but non-toxic for Daphnia magna, a key aquatic species. Compound 1 exhibited the lowest bioaccumulation factor value (0.17). All compounds were inactive against Tox21-Stress response pathways, but compound 2 and 3 could potentially be immunotoxic. Finally, all tested compounds may possess a non persistent profile, while only compound 1 and 4 could possibly be biodegradable. This preliminary work, revealed once again the potential of phyto-compounds and certain fundamental aspects, which could represents an initial step for further investigation.
临床药理学和毒理学如今被认为是两个相辅相成、不可或缺的医学学科,使临床医生能够实现几种物质的最佳治疗效果,同时也能表征其毒性阈值。生物信息学工具和数据库在这两个领域的贡献提供了关键要素,有时在早期临床阶段无法解释。在这种情况下,本工作使用了一些计算方法来研究四种天然化合物的分子量分布(MWD)、致敏性、毒性、可能的生物降解、持久性和生物累积因子。结果表明,化合物2和3的MWD指数在测试化合物中最好。然而,化合物1的给药途径可以被认为是对大鼠最安全的,尤其是口服、静脉内和皮下给药途径,其中与其他化合物相比,LD50值最好。两项致敏性测试显示,这四种植物化合物可被视为非致敏剂,可能不会产生任何药物毒性风险。关于细胞毒性,化合物4在这些化合物中表现出最好的细胞毒性作用,其对结肠癌(HCT-116)细胞系的相应值为Pa=0.782。这四种生物化合物可能对鸟类有毒,但对重要水生物种大型瑞香无毒。化合物1表现出最低的生物累积因子值(0.17)。所有化合物对Tox21应激反应途径都没有活性,但化合物2和3可能具有免疫毒性。最后,所有测试的化合物都可能具有非持久性,而只有化合物1和4可能是可生物降解的。这项初步工作再次揭示了植物化合物的潜力和某些基本方面,这可能是进一步研究的第一步。
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引用次数: 0
Remote Sensing-Based Assessment of Soil and Water Pollution in Deep Excavation Scenario 基于遥感的深基坑土壤和水体污染评价
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2289
Binbin Qiao, Zhenghua Leng, Shixiang Mao, Qiang Wang, Han Liu
Soil and water resource mismanagement can negatively intensify global poverty and jeopardize ecology. Soil can be contaminated by heavy metals, organic chemicals like biological pathogens, pesticides, micro plastics and nano particles. Contamination decreases the soil’s capability to yield food thereby affecting food production by means of pollution and disease. Moreover, soil contaminants move into rivers damaging the water quality. Numerous technologies have been established to tackle water and soil remediation, such as deep excavation technique where transportation of contaminated soils or water is done to remote unpopulated sites. Recent development in Remote Sensing and geographic information processing techniques have led to exciting new opportunities for investigating and closely monitoring environmental factors that influence key land and soil management approaches. Hence, this paper uses the Deep Excavation and Remote Sensing based Assessment Model (DERSAM) to assess the soil and water pollution in contaminated areas. The data are taken from the Europe soil contamination map to classify the contaminated and non-contaminated areas. This data obtained from the high-resolution satellite of Remote Sensing (RS) technique and Geographical Information System (GIS) with the parallel expansion of a fully assimilated geospatial database system that provides monitoring and feedback at suitable spatial scales. Thus, such data can be utilized for long-term environmental management and monitoring of remediation and rehabilitation of excavation areas. The numerical outcomes show that the recommended DERSAM model increases the land use and land cover change prediction by 90.2%, the classification ratio by 98.2%, the pollution reduction ratio by 96.6%, the soil contamination detection ratio by 95.9%, and the overall performance by 97.2% compared to other existing approaches.
水土资源管理不善会加剧全球贫困并危害生态。土壤可能受到重金属、生物病原体等有机化学物质、农药、微塑料和纳米颗粒的污染。污染降低了土壤生产粮食的能力,从而通过污染和疾病影响粮食生产。此外,土壤污染物进入河流破坏水质。已经建立了许多技术来解决水和土壤修复问题,例如将受污染的土壤或水运输到偏远无人居住的地方的深开挖技术。遥感和地理信息处理技术的最新发展为调查和密切监测影响关键土地和土壤管理方法的环境因素带来了令人兴奋的新机会。因此,本文采用基于深度开挖和遥感的评价模型(DERSAM)对污染区域的土壤和水污染进行评价。数据取自欧洲土壤污染图,用于对污染区域和非污染区域进行分类。这些数据来自遥感技术的高分辨率卫星和地理信息系统,并同时扩展了一个完全同化的地理空间数据库系统,在适当的空间尺度上提供监测和反馈。因此,这些数据可用于长期的环境管理和监测开挖区域的修复和恢复。数值结果表明,与其他方法相比,推荐的DERSAM模型的土地利用和土地覆盖变化预测提高90.2%,分类率提高98.2%,污染减少率提高96.6%,土壤污染检测率提高95.9%,总体性能提高97.2%。
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引用次数: 0
A Property-Response Approach to Evaluate Acute Toxicity Profile and Pharmacological Quality of Hydro-Alcoholic Extract of Walnut Root Bark Juglans Regia Linn. in Streptozotocin-Induced Diabetic Mode 评价核桃根皮水醇提取物急性毒性和药理质量的性质-反应法。链脲佐菌素诱导的糖尿病模式
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2288
Mohamed Akram Melakhessou, Imène Becheker, Salah Eddine Marref, M. Bensaad, A. Alyamani, R. Sami, M. Helal, Abeer M. Aljaadi, Rasha A. Al-Eisa, Naseh A. Algehainy, Faisal H. Altemani
Juglans regia L., commonly known as walnut, is a valuable medicinal herb with the potency to treat various conditions and illnesses including diabetes, rheumatism, fever and skin illnesses. This study assessed the acute toxicity, anti-diabetic and anti-atherogenic activities of the hydro-alcoholic extract of the walnut root bark of Juglans regia L. (Juglandaceae), in vivo, using Wistar rats. The toxicological effects of the hydro-alcoholic extract of walnut bark Juglans regia L: are still elusive. In order to evaluate the toxicity profile of this plant, rats were orally treated with a single concentration of 2000 mg/kg and observed during a period of two weeks. For the anti-diabetic study, thirty male wistar rats (130–170 g) were randomized into 5 groups (n = 6/group). Groups I and II served as negative and normal controls, respectively. Diabetes was induced in test groups (II–V) using 200 mg/kg of body weight (BW) streptozotocin. Concerning the clincial outcomes, no mortality, morbidity, or abnormal hematological, biochemical and histopathological alterations were observed. Accordingly, J. regia L is considered a non-toxic plant. The extract was found to limit weight loss and reduce blood glucose levels by −32.30% after 14 days of treatment for the anti-diabetic and anti-atherogenic study. The extract also reduced dyslipidaemia. Our data indicated that J. regia L contains bio-compounds that may alleviate chronic hyperglycemia while preventing cardiovascular complications by improving dyslipidaemia. This could be a potential herb for future studies to develop more effective drugs for improving glycemic and cholesterol control.
核桃,俗称胡桃,是一种珍贵的草药,具有治疗糖尿病、风湿病、发烧和皮肤病等多种疾病的功效。本研究使用Wistar大鼠在体内评估了核桃科核桃根皮水醇提取物的急性毒性、抗糖尿病和抗动脉粥样硬化活性。核桃皮核桃水醇提取物的毒理学作用仍然难以捉摸。为了评估这种植物的毒性特征,用2000 mg/kg的单一浓度口服大鼠,并在两周内观察。在抗糖尿病研究中,30只雄性wistar大鼠(130-170 g)被随机分为5组(n=6/组)。第一组和第二组分别作为阴性对照和正常对照。试验组(II–V)使用200 mg/kg体重的链脲佐菌素诱导糖尿病。关于临床结果,未观察到死亡率、发病率或异常血液学、生化和组织病理学改变。因此,王被认为是一种无毒植物。在抗糖尿病和抗动脉粥样硬化研究中,发现该提取物在治疗14天后可以限制体重减轻并降低−32.30%的血糖水平。提取物还可以减少血脂异常。我们的数据表明,J.regia L含有生物化合物,可以缓解慢性高血糖,同时通过改善血脂异常来预防心血管并发症。这可能是一种潜在的草药,用于未来的研究,以开发更有效的药物来改善血糖和胆固醇控制。
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引用次数: 0
Facile Synthesis of Melamine/G-C3N4 Covalent Organic Framework (COF) by Indirect Intercalation and Its Flame Retardant 间接插层法合成三聚氰胺/G-C3N4共价有机骨架及其阻燃剂
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2297
Liqin Shen, Boqiang Xu, Hengming Hu, Zichun Lv, L. Ben
In this paper, a covalent organic framework (COF) based on g-C3N4 was designed firstly. The COF is arranged through an indirect interrelation method among melamine and g-C3N4. During the reaction, Cu2+ was used to expand the layer space of g-C3N4. The as-prepared COF were characterized by SEM, TEM, FTIR, and XRD. The obtained product is a melamine interrelation g-C3N4 composite material (MCN) with COF structure. The MCN was used as flame retardant for cable sheath material. The thermal stability, thermal shrinkage, oxygen record and vertical burning of link sheath material contain MCN were tested. The results showed that the MCN flame retardant increases the thermal stability time of the sheath material from 89 min to 131 min, and the thermal shrinkage rate diminished from 4.17% to 2.00%. The LOI is 29.5, and the flame retardant grade reaches UL-94 V-0. The SEM analysis of the carbon residue of the cable sheath material after burning, it was found that the state of the carbon layer of the link sheath material transform into a thick honeycomb structure by the impact of the MCN flame retardant, which forestalls the dispersion of oxygen and burnable gases, in this way hindering the transmission of flame.
本文首先设计了一种基于g-C3N4的共价有机骨架。COF是通过三聚氰胺和g-C3N4之间的间接相互关系方法排列的。在反应过程中,Cu2+被用来扩大g-C3N4的层空间。通过SEM、TEM、FTIR和XRD对所制备的COF进行了表征。所获得的产物是具有COF结构的三聚氰胺相互作用的g-C3N4复合材料(MCN)。MCN被用作电缆护套材料的阻燃剂。测试了含有MCN的链路护套材料的热稳定性、热收缩性、氧记录和垂直燃烧性能。结果表明,MCN阻燃剂使护套材料的热稳定时间从89min增加到131min,热收缩率从4.17%降低到2.00%,LOI为29.5,阻燃等级达到UL-94V-0。对电缆护套材料燃烧后的碳残留物进行SEM分析发现,在MCN阻燃剂的影响下,连接护套材料的碳层状态转变为厚蜂窝结构,阻止了氧气和可燃气体的扩散,从而阻碍了火焰的传播。
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引用次数: 0
Impact of Coexistence of Microplastics and Biochar on the Abundance and Structure of Soil Fungal Communities 微塑料与生物炭共存对土壤真菌群落丰度和结构的影响
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2294
Xiying Wang, Hui Zhao, Deyan Li, Zhiyong Tan, Jianwei Hou
In this pursuit, we analyzed the effects of microplastics (PP, PE, PVC) and microplastics and biochar coexistence (PPR, PER, PVCR) on soil fungal community structure and diversity, and functional prediction analysis. Results showed that microplastics and their coexistence with biochar had a significant impact on the soil physicochemical, with PVCR treatment exhibiting the highest soil pH, organic carbon, phosphorus, potassium, and ammonium nitrogen content. Microplastics and their coexistence with biochar significantly increased fungal abundance, with PPR treatment having the highest fungal abundance. Microplastics and their coexistence with biochar (except for PP) significantly reduced the soil fungal diversity indices. The dominant fungal communities were Ascomycota, Basidiomycota, and Zygomycota, while at the genus level, Mortierella, Aspergillus, and Fusarium were found to be dominant taxa. Microplastics and their coexistence with biochar promoted the Basidiomycota and Mortierella and inhibited the Ascomycota and Fusarium. Effect of microplastics and biochar coexistence was significantly higher than that of microplastics alone. Soil pH, ammonium nitrogen, organic carbon, nitrate nitrogen, and potassium were main factors affecting the soil fungal community structure changes. FUNGuild functional prediction results showed that microplastics and their coexistence with biochar had a significant influence on the functional group. The relative abundance of pathogenic-saprotrophic-symbiotic nutritional fungi was the highest in the PPR while the PVCR showed the highest relative abundance of saprotrophic nutritional fungi. In summary, the coexistence of microplastics and biochar had a significant affect the soil fungal community, while its impact exhibited variations depending on the type of microplastics.
为此,我们分析了微塑料(PP、PE、PVC)和微塑料与生物炭共存(PPR、PER、PVCR)对土壤真菌群落结构和多样性的影响,并进行了功能预测分析。结果表明,微塑料及其与生物炭共存对土壤理化性质有显著影响,其中PVCR处理土壤pH、有机碳、磷、钾和铵态氮含量最高。微塑料及其与生物炭的共存显著提高了真菌丰度,其中PPR处理真菌丰度最高。微塑料及其与生物炭的共存显著降低了土壤真菌多样性指数(PP除外)。优势真菌群落为子囊菌门、担子菌门和合菌门,属水平上优势真菌类群为Mortierella、Aspergillus和Fusarium。微塑料及其与生物炭的共存促进担子菌群和摩氏菌群的生长,抑制子囊菌群和镰刀菌群的生长。微塑料与生物炭共存的影响显著高于微塑料单独存在的影响。土壤pH、铵态氮、有机碳、硝态氮和钾是影响土壤真菌群落结构变化的主要因素。FUNGuild功能预测结果表明,微塑料及其与生物炭共存对其官能团有显著影响。致病性-腐坏性-共生性营养真菌的相对丰度在PPR中最高,而PVCR中腐坏性营养真菌的相对丰度最高。综上所述,微塑料与生物炭共存对土壤真菌群落有显著影响,但其影响因微塑料类型而异。
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引用次数: 0
The Niche Mechanism of Weeds in Cotton Field Under Drip-Irrigated in North Xinjiang, China 北疆滴灌棉田杂草生态位机制研究
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2292
Lizhong Sun, Jing Chen, Xiujin Hu, Tao Guo, Tong Liu
The aim of this study was to investigate the niche mechanism and diversity of weeds in drip-irrigated and machine-harvested cotton fields in north Xinjiang, China. The dominant classes of weeds in 25 cotton fields under drip-irrigated and machine-harvested in 5 regions of north Xinjiang were investigated by seven-grade visual method, and converted to important value data, the 20 main weeds niche breadth and niche overlap were caculated on the basis of the data above. The ecological similiarity revealed by DCA sorting and minimum spanning tree of graph theory cluster analysis is drawed on the basis of the niche overlap value. The results show that Convolvulus arvensis L., Chenopodium album L., Amaranthus retroflexus L., Solanum nigrum L., Phragmites australis (Cav.) Trin. ex Steud., Setria viridis (L.) Beauv., Abutilon theophrasti Medicus., Hibiscus trionum L., Echinochloa crusgalli (L.) Beauv., nine species of weeds, which are the dominant species (malignant weeds) in drip-irrigated and machine-harvested cotton fields in North Xinjiang, have wide niche. The niche overlap values of S. nigrum L. and C. album L., A. theophrasti Medicus. and A. retroflexus L., H. trionum L. and E. crusgall (L.) Beauv., A. retroflexus L. and E. crusgalli (L.) Beauv. are higher. The minimal spanning tree method based on niche overlap value to reflect similar degree of the ecological needs of the weeds, 20 main weeds will be divided into 4 groups. DCA ordination of 20 main weeds comprehensive ecological needs of 25 sampling sites: the vicious weeds all gathered at the central sorting chart, the distinction is obvious with the general weed and the corresponding of DCA ordination chart with minimal spanning tree is better. The minimal spanning tree by its focus on the results of which show that weeds are the most similar to the ecological requirements, but not be used for the no adjacent species, and no clear that the ecological relationship between them, the DCA sorting can reflect the ecology similar relations between all weeds wholely, though this method will lose a small amount of ecological information through the data conversion. Therefore, the two methods will be combined, complementary advantages, the superiority is obvious. Along with drip irrigation age years increase, S. nigrum L., C. arvensis L., C. album L. and so on, between dominant value of nine species of malignant weeds and the drip irrigation age years has the remarkable regression relations.
本研究旨在探讨北疆棉田滴灌和机收棉田杂草的生态位机制和多样性。采用七级可视化方法对新疆北部5个地区25个滴灌机收棉田杂草的优势类群进行了调查,并将其转化为重要值数据,在此基础上计算出20种主要杂草的生态位宽度和生态位重叠。在生态位重叠值的基础上,得出了DCA排序和图论聚类分析的最小生成树所揭示的生态相似性。结果表明:盘花、藜、反曲苋、龙葵、芦苇(Cav.)Trin。前Steud。,Setria viridis(L.)Beauv。,阿布蒂隆·泰奥弗拉斯蒂·梅迪库斯。,木槿属。,九种杂草是北疆棉田滴灌和机收的优势种(恶性杂草),具有广阔的生态位。S.nigrum L.和C.album L.、A.theoprasti Medicus的生态位重叠值。以及A.retroflexus L.、H.trinum L.和E.crusgall(L.)Beauv。,A.retroflexus L.和E.crugalli(L.)Beauv。更高。基于生态位重叠值的最小生成树方法反映了杂草生态需求的相似程度,将20种主要杂草分为4组。25个采样点20种主要杂草的DCA排序综合生态需求:恶性杂草均集中在中央排序图上,与一般杂草的区别明显,最小生成树DCA排序图对应较好。最小生成树通过其关注的结果表明,杂草最符合生态要求,但不适用于没有相邻物种,也不清楚它们之间的生态关系,DCA排序可以完整地反映所有杂草之间的生态相似关系,尽管这种方法会通过数据转换损失少量的生态信息。因此,将两种方法结合起来,优势互补,优势明显。随着滴灌年龄年的增加,黑穗草、珙桐、珙桐等9种恶性杂草的优势值与滴灌年龄年之间存在显著的回归关系。
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引用次数: 0
The Effects of Cr/Cd Contamination on Vegetable Cultivars in Hainan Province Cr/Cd污染对海南省蔬菜品种的影响
IF 0.5 4区 医学 Pub Date : 2023-08-01 DOI: 10.1166/jbmb.2023.2299
Yujia Wu, Dongming Zhang, Yongping Huang, Kaijun Chang, Jinyuan Wen, Wen Zhang
Cadmium (Cd) and chromium (Cr), as nonessential elements, are considered as very important pollutants, which greatly affects human health. To reduce food chain risk for vegetable soils contaminated by Cd, and/or Cr, the pot experiments were conducted to investigate Cd or Cr uptake by 9 cultivars of familiar leaf vegetables and to screen leaf vegetables with lower Cd or Cr content in Hainan Province. The results showed that the leaf Cd or Cr contents of 8 vegetables were lower than national limit of contaminants in foods under the treatments of 0.61 mg/kg Cd and 3.07 mg/kg Cd. Only for Lactuca sativa var. longifoliaf, Cd accumulation (fresh weight, 0.2275 mg/kg) exceeded the limit of Cd (0.2 mg/kg) under the treatments (3.07 mg/kg Cd and 104 mg/kg Cr). About Cr accumulation, all tested vegetables were lower than national limit of Cr (0.5 mg/kg). Considering the yield and Cd content (fresh weight), Brassica rapa ssp. chinensis (181.5 g, 0.0172 mg/kg) has the greatest advantage. Brassica pekinensis (11.824 g, 0.0204 mg/kg), Brassica campestris ssp. chinensis 328 (11.8 g, 0.0129 mg/kg) and Lactuca sativa var. ramose (8.2 g, 0.0144 mg/kg) have also the relative advantage.
镉(Cd)和铬(Cr)作为非必需元素,被认为是非常重要的污染物,极大地影响着人类的健康。为降低蔬菜土壤Cd、Cr污染的食物链风险,采用盆栽试验研究了海南省9个常见叶菜品种对Cd、Cr的吸收情况,筛选了Cd、Cr含量较低的叶菜品种。结果表明,8种蔬菜在0.61 mg/kg Cd和3.07 mg/kg Cd处理下,叶片Cd或Cr含量均低于国家食品污染物限量,只有长叶莴苣的Cd积累量(鲜重0.2275 mg/kg)在3.07 mg/kg Cd和104 mg/kg Cr处理下超过了Cd限量(0.2 mg/kg)。在Cr积累方面,所有蔬菜均低于Cr国家限量(0.5 mg/kg)。从产量和Cd含量(鲜重)两方面考虑,对油菜品种进行了改良。紫草(181.5 g, 0.0172 mg/kg)优势最大。北京芸苔(11.824 g, 0.0204 mg/kg);中国草芥328 (11.8 g, 0.0129 mg/kg)和芥花(8.2 g, 0.0144 mg/kg)也具有相对优势。
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引用次数: 0
Ecofriendly Management of Root Knot Nematode (Meloidogyne incognita) in Okra (Abelmoschus esculentus (L.) Moench) 秋葵根结线虫的生态治理Moench)
IF 0.5 4区 医学 Pub Date : 2023-06-01 DOI: 10.1166/jbmb.2023.2286
H. Pant, A. Maurya, Aditya, M. Singh, Vinny John, Manika Mehra, R. Sami, Fadi Baakdah, M. Helal
The pertinence of bio-control agents applied with plant-based materials is a highly efficient and reliable sustainable management practices in agriculture. The present study dealt with the repercussion of various bio-control agents and oil seed cakes viz., Trichoderma viride, neem cake, T. viride+neem cake, Paecilomyces lilacinus, Beauveria bassiana, T. harzianum, soybean cake and castor cake were used against root knot nematode (Meloidogyne incognita) in okra (Abelmoschus esculentus (L.) Moench) crop. The results showed, all of the bio-control agents and oil seed cakes, either used alone or in combination, suppressed the galls of the root knot nematode. Significant growth parameters and yield of the fruits were perceived in plants treated with combination of T. viride+neem cake followed by P. lilacinus, T. viride, T. harzianum, neem cake, B. bassiana, soybean cake and castor cake respectively. Merest root galls were found in a T. viride+neem cake combination. T. viride was the most effective bioagent and in case of oil seed cakes, neem cake was the most effective in improving growth parameters and reducing root galls caused by M. incognita in the okra crop.
利用植物性材料应用生物防治剂的针对性是一种高效可靠的农业可持续管理实践。本研究采用绿色木霉、印楝饼、绿色绿脓杆菌+印楝饼,淡紫色拟青霉、球孢白僵菌、哈茨霉、大豆饼和蓖麻饼等生物防治剂和油菜籽饼对秋葵(Abelmoschus esculentus(L.)Moench)作物根结线虫(Meloidogyne incognita)的防治效果。结果表明,无论是单独使用还是联合使用,所有生物防治剂和油籽饼都能抑制根结线虫的虫蛀。以T.viride+印楝饼组合处理的植株,其生长参数和果实产量显著,其次分别为紫丁香、T.viride、T.harzianum、印楝饼、B.bassiana、大豆饼和蓖麻饼。在T.viride+印楝饼的组合中发现了单纯的根结石。T.viride是最有效的生物制剂,在油籽饼的情况下,印楝饼在改善秋葵作物生长参数和减少由M.incognita引起的根结石方面最有效。
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引用次数: 0
Environmental Impact of Wasteyard Leachate Pollution, It’s Health Risks with Some Microbial and Ecological Implications 垃圾渗滤液污染的环境影响及其健康风险及其微生物和生态意义
IF 0.5 4区 医学 Pub Date : 2023-06-01 DOI: 10.1166/jbmb.2023.2282
H. Uguru, A. Essaghah, O.M. Akwenuke, O. Akpokodje, R. Sami, M. Helal, Roqayah H. Kadi
The study was carried out to assess the public health risks from the consumption of farm crops and vegetables cultivated around active municipal solid waste dumpsites in Delta State. Using the experimental research method soil and vegetation (cassava leaves) samples were collected from ten spatial locations around municipal solid waste dumpsites (SWD), scrap metals yards (SMY) and agricultural farms and subjected to laboratory analysis of relevant physic-chemical parameters. Heavy metals like cadmium “Cd”, iron “Fe”, nickel “Ni” and lead “Pb” concentrations including, total variable bacteria (TVB) and Salmonella spp load in collected samples were determined in accordance with standard procedures. The results revealed a substantial spatial dispersion of the heavy metals, TVB and Salmonella spp concentrations within the two study areas. Remarkably, the bacteria level was higher in the SWD than in the SMY while the heavy metals concentration was higher in the SMYs when compared to the SWD. Spatially, soil and vegetation around the SMD area had higher HMs’ Hazard quotient, Hazard index and Carcinogenic risk values, when compared to the soil around the SMY region, but all their status were within safe limits for the human health. Salmonella spp population in the SWD and SMY soil ranged from 1.33 to 39.33 cfu/g of dry soil and 1.04 to 8.67 cfu/g of dry soil respectively while the TVB count ranged from 6233 cfu/g to 46500 cfu/g of dry soil. Although the heavy metal contamination levels in the soil samples were within safe limits, the presence of the pathogenic bacterium in the soil pose health threat to the inhabitants of the regions.
进行这项研究是为了评估食用三角洲州活跃的城市固体废物倾倒场周围种植的农作物和蔬菜所带来的公共健康风险。采用实验研究方法,在城市生活垃圾填埋场(SWD)、废金属堆场(SMY)和农田周围的10个空间点采集土壤和植被(木薯叶)样品,并进行相关理化参数的实验室分析。按标准程序测定样品中镉“Cd”、铁“Fe”、镍“Ni”和铅“Pb”等重金属浓度,包括总可变菌(TVB)和沙门氏菌(Salmonella)载量。结果表明,重金属、TVB和沙门氏菌浓度在两个研究区内具有明显的空间分布。值得注意的是,SWD中的细菌水平高于SMY,而SMYs中的重金属浓度高于SWD。从空间上看,SMD区周边土壤和植被的HMs危害系数、危害指数和致癌风险值均高于SMY区周边土壤,但均处于人体健康安全范围内。沙门菌种群数量在干土1.33 ~ 39.33 cfu/g和干土1.04 ~ 8.67 cfu/g之间,TVB种群数量在干土6233 ~ 46500 cfu/g之间。虽然土壤样品中的重金属污染水平在安全范围内,但土壤中致病菌的存在对该地区居民的健康构成威胁。
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引用次数: 0
Construction of Longan (Dimocarpus longan Lour.) Simple Sequence Repeat Fingerprints and Application in Distinctness, Uniformity and Stability Testing 龙眼(Dimocarpus Longan Lour.)的构造简单序列重复指纹图谱及其在鉴别性、均匀性和稳定性检测中的应用
IF 0.5 4区 医学 Pub Date : 2023-06-01 DOI: 10.1166/jbmb.2023.2274
Q. Ma, Mengqiang Chen, Hong Liu, Liqing Liu, Jiwei Yin, Xi Yang, Jialin Lv, D. Rao, S. Shi, Zhenjiang Xu
Simple sequence repeat (SSR) markers are highly polymorphism, good reproducibility, abundant number and co-dominant inheritance, and were considered to be one of the preferred molecular markers for DNA fingerprints in distinctness, uniformity and stability (DUS) testing. In this study, 10 representative Dimocarpus longan Lour (longan) varieties with significant differences were selected from 63 longan varieties according to the morphological characteristics. Based on PCR amplifications of the 10 selected varieties, 24 SSR primers pairs were screened from total 300 SSR primers pairs, to establish SSR fingerprints for all 63 longan varieties. The results showed that a total of 127 alleles were detected in 63 longan varieties, with an average of 5.29 alleles for each pair of primers. The Shannon’s index of the 24 pairs of SSR markers ranged from 0.64 to 1.58, with an average of 1.20. The polymorphism information content of each locus ranged from 0.32 to 0.72, with an average of 0.58. Clustering analysis indicated that most of the varieties with close genetic relationships tended to fall in the same cluster, and only a few in different clusters or sub-clusters. The 63 longan varieties were completely identified by the optimal combination of 6 pairs of SSR primers (LY161, LY252, LY137, LY130, LY25 and LY34). Overall, DNA fingerprints of the 63 longan varieties were constructed based on these 24 pairs of SSR primers. This study may provide a technical support for variety identification and similar variety screening in DUS detection in longan.
简单序列重复(SSR)标记具有多态性高、重现性好、数量丰富、共显性遗传等特点,是DNA指纹鉴别、均匀性和稳定性(DUS)检测的首选分子标记之一。本研究根据形态特征,从63个龙眼品种中筛选出10个具有代表性、差异显著的龙眼品种。根据10个选择品种的PCR扩增结果,从300对SSR引物中筛选出24对,建立了63个龙眼品种的SSR指纹图谱。结果表明,63个龙眼品种共检测到127个等位基因,平均每对引物检测到5.29个等位基因。24对SSR标记的香农指数范围为0.64 ~ 1.58,平均为1.20。每个位点的多态性信息含量在0.32 ~ 0.72之间,平均为0.58。聚类分析结果表明,亲缘关系较近的品种多数属于同一聚类,少数属于不同聚类或亚聚类。通过6对SSR引物LY161、LY252、LY137、LY130、LY25和LY34的最优组合,对63个龙眼品种进行了完整的鉴定。利用这24对SSR引物构建了63个龙眼品种的DNA指纹图谱。本研究可为龙眼DUS检测中的品种鉴定和同类品种筛选提供技术支持。
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引用次数: 0
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Journal of Biobased Materials and Bioenergy
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