This research has studied the addition effect of woven carbon fiber treated with NaOH and coated by β- TCP to the conventional polymethyl methacrylate (PMMA). Two stages are involved in the preparation of heat-cured acrylic denture composite. The first chemical treatment of woven carbon fiber (WCF) surface by different concentrations of NaOH (0.10, 0.15, and 0.20 M) and coating carbon fiber with β-TCP by various weight fractions (0.08, 0.10, and 0.12 wi) were carried out. The second stage includes the reinforcement of denture base material by treated and coated WCF. The functional groups of the WCF surface before and after alkali treatment were studied by FTIR. The morphology of the WCF surface before and after alkali treatment was observed by FESEM, the diameter of pores on the untreated and treated fibers, and the adhesion of β-TCP powder to the fiber was also observed. Mechanical tests include: impact strength (I.S.) and flexural strength (F.S) were calculated using a three-point bending test with a universal test machine and a Charpy impact test machine, respectively. From the result, reinforcing PMMA with treated and coated WCF improves the mechanical properties (impact and flexural strength).
{"title":"Incorporation of Treated Woven Carbon Fiber to Methacrylate Resin for Heat-Cured Acrylic Denture Composite","authors":"Wafaa A. Hussain, M. Ismail, S. Taher","doi":"10.4028/p-627g18","DOIUrl":"https://doi.org/10.4028/p-627g18","url":null,"abstract":"This research has studied the addition effect of woven carbon fiber treated with NaOH and coated by β- TCP to the conventional polymethyl methacrylate (PMMA). Two stages are involved in the preparation of heat-cured acrylic denture composite. The first chemical treatment of woven carbon fiber (WCF) surface by different concentrations of NaOH (0.10, 0.15, and 0.20 M) and coating carbon fiber with β-TCP by various weight fractions (0.08, 0.10, and 0.12 wi) were carried out. The second stage includes the reinforcement of denture base material by treated and coated WCF. The functional groups of the WCF surface before and after alkali treatment were studied by FTIR. The morphology of the WCF surface before and after alkali treatment was observed by FESEM, the diameter of pores on the untreated and treated fibers, and the adhesion of β-TCP powder to the fiber was also observed. Mechanical tests include: impact strength (I.S.) and flexural strength (F.S) were calculated using a three-point bending test with a universal test machine and a Charpy impact test machine, respectively. From the result, reinforcing PMMA with treated and coated WCF improves the mechanical properties (impact and flexural strength).","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"153 - 164"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45065978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The cervical spine is a structure subject to various vertebral injuries, namely, herniation of intervertebral discs and osteoporosis. Nowadays, several segments of society are vulnerable to these diseases that affect spine motion especially elderly people and women. Hence, various designs of cervical artificial discs are in use or under investigation claiming to restore the normal kinematics of the cervical spine. In this work, it is proposed to minimize the stress level by numerical size optimization in the Mobi-C cervical spine prosthesis to improve their biomechanical performances. For this aim, design of experiment (DoE) is employed as an optimization technique to investigate three geometrical parameters of the prosthesis design. Accordingly, DoE optimization allowed to minimize the equivalent stress value on Mobi-C from 20.3 MPa to 17.856 MPa corresponding to a percentage decrease of 12% from the original geometry. This provides an advantage for the durability of the prosthesis and also for the bone by reducing stress concentration.
{"title":"Numerical Size Optimization of Cervical Spine Disc Prosthesis Mobi-C Using Design of Experiment Technics","authors":"K. Chenaifi, Cherif Ali Messellek, Y. Benabid","doi":"10.4028/p-jo58gu","DOIUrl":"https://doi.org/10.4028/p-jo58gu","url":null,"abstract":"The cervical spine is a structure subject to various vertebral injuries, namely, herniation of intervertebral discs and osteoporosis. Nowadays, several segments of society are vulnerable to these diseases that affect spine motion especially elderly people and women. Hence, various designs of cervical artificial discs are in use or under investigation claiming to restore the normal kinematics of the cervical spine. In this work, it is proposed to minimize the stress level by numerical size optimization in the Mobi-C cervical spine prosthesis to improve their biomechanical performances. For this aim, design of experiment (DoE) is employed as an optimization technique to investigate three geometrical parameters of the prosthesis design. Accordingly, DoE optimization allowed to minimize the equivalent stress value on Mobi-C from 20.3 MPa to 17.856 MPa corresponding to a percentage decrease of 12% from the original geometry. This provides an advantage for the durability of the prosthesis and also for the bone by reducing stress concentration.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"173 - 188"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41517255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zahra Keykhaee, Amin Bahmani, N. Sattarahmady, Marzieh Ghanbari Jahromi, E. Shokri
In the present study, a simple and fast approach was developed for the green synthesis of silver nanoparticles by using Eryngium campestre (Eryngo) extract prepared in boiling water. People have widely used the Eryngo plant as a vegetable, food, and medicine around the world. The dried leaves of Eryngo extracted in boiling water yielded approximately 67 mg/g (6.6%) solid residue. The extract had a high antioxidant activity of 71 %, which was rich in total phenolic and flavonoids as revealed through colorimetric assays. For preparing nanoparticles, silver nitrate was added to the plant extract diluents and kept until the solution color changed with a sharp indicative peak of AgNPs that appeared at 450 nm. In addition, UV/Vis, TEM, FESEM, DLS, EDS, and XRD analysis were used to characterize the as-synthesized AgNPs. The results confirmed the spherical shape and nano nature of AgNPs with an average size of 32 nm based on Fe-SEM and TEM observations. The prepared AgNPs also shown moderate free radical scavenging activity (60%) in DPPH test and exhibit antibacterial activity at low concentration (50 μg/mL) toward both gram-positive and gram-negative bacteria. In this respect, the inhibition zone was higher in gram-positive bacteria and the sensitivity order of S.aureus > MRSA > B.subtilis > P.aeruginosa > E.coli was achieved in response to Eryngo AgNPs. Interestingly, Eryngo AgNPs at low concentration were efficient on MRSA, as an antibiotic-resistant strain of S.aureus.
{"title":"Eryngo Extract-Mediated Green Synthesis of Silver Nanoparticles and its Antibacterial Activity against Resistance Strains","authors":"Zahra Keykhaee, Amin Bahmani, N. Sattarahmady, Marzieh Ghanbari Jahromi, E. Shokri","doi":"10.4028/p-z4tf46","DOIUrl":"https://doi.org/10.4028/p-z4tf46","url":null,"abstract":"In the present study, a simple and fast approach was developed for the green synthesis of silver nanoparticles by using Eryngium campestre (Eryngo) extract prepared in boiling water. People have widely used the Eryngo plant as a vegetable, food, and medicine around the world. The dried leaves of Eryngo extracted in boiling water yielded approximately 67 mg/g (6.6%) solid residue. The extract had a high antioxidant activity of 71 %, which was rich in total phenolic and flavonoids as revealed through colorimetric assays. For preparing nanoparticles, silver nitrate was added to the plant extract diluents and kept until the solution color changed with a sharp indicative peak of AgNPs that appeared at 450 nm. In addition, UV/Vis, TEM, FESEM, DLS, EDS, and XRD analysis were used to characterize the as-synthesized AgNPs. The results confirmed the spherical shape and nano nature of AgNPs with an average size of 32 nm based on Fe-SEM and TEM observations. The prepared AgNPs also shown moderate free radical scavenging activity (60%) in DPPH test and exhibit antibacterial activity at low concentration (50 μg/mL) toward both gram-positive and gram-negative bacteria. In this respect, the inhibition zone was higher in gram-positive bacteria and the sensitivity order of S.aureus > MRSA > B.subtilis > P.aeruginosa > E.coli was achieved in response to Eryngo AgNPs. Interestingly, Eryngo AgNPs at low concentration were efficient on MRSA, as an antibiotic-resistant strain of S.aureus.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"61 - 73"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49304186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kulkarni, Yogesh Wadhavane, Y. Chendake, A. Kulkarni
Cellulose based nanofilms have large applications in biomedical and related fields due to their antimicrobial properties. Their applicability depends upon purity of cellulose, composition, and structural properties of films. The nanofilms of cellulose extracted from Water Hyacinth (Eichhornia crassipes) and Roselle (Hibiscus Sabdariffa) plant possesses excellent properties for biomedical applications due to their biological origin and ZnO or other metal loading properties. Microwave assisted physical separation of cellulose provided excellent films formation properties and ZnO loading compared without any chemical traces. The presence of chemical impurities to affects structural, morphological properties and contact angle. It affects the biomedical applicability of cellulose based films. The microwave-based extraction was further assisted by use of polyethylene glycol with molecular weight 600, which increases the solubility and extractability of cellulose to 90 %. Formed films showed higher contact angle and hydrophobicity. This increased hydrophobicity of cellulosic nanofilms showed enhanced antimicrobial activities towards gram-negative and gram-positive bacteria by water hyacinth nanofilms. Thus, microwave-based synthesis of cellulose nanofilms resulted into enhanced microbial activities.
{"title":"Microwave Assisted Synthesis of Antimicrobial Nano-Films from Water Hyacinth (Eichhornia crassipes) and Roselle (Hibiscus sabdariffa) Plant Extract","authors":"K. Kulkarni, Yogesh Wadhavane, Y. Chendake, A. Kulkarni","doi":"10.4028/p-ixf3fr","DOIUrl":"https://doi.org/10.4028/p-ixf3fr","url":null,"abstract":"Cellulose based nanofilms have large applications in biomedical and related fields due to their antimicrobial properties. Their applicability depends upon purity of cellulose, composition, and structural properties of films. The nanofilms of cellulose extracted from Water Hyacinth (Eichhornia crassipes) and Roselle (Hibiscus Sabdariffa) plant possesses excellent properties for biomedical applications due to their biological origin and ZnO or other metal loading properties. Microwave assisted physical separation of cellulose provided excellent films formation properties and ZnO loading compared without any chemical traces. The presence of chemical impurities to affects structural, morphological properties and contact angle. It affects the biomedical applicability of cellulose based films. The microwave-based extraction was further assisted by use of polyethylene glycol with molecular weight 600, which increases the solubility and extractability of cellulose to 90 %. Formed films showed higher contact angle and hydrophobicity. This increased hydrophobicity of cellulosic nanofilms showed enhanced antimicrobial activities towards gram-negative and gram-positive bacteria by water hyacinth nanofilms. Thus, microwave-based synthesis of cellulose nanofilms resulted into enhanced microbial activities.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"37 - 48"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46282453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Felipe Sampaio-Jorge, Anderson P Morales, Alexandre Miranda Rodrigues, M. Magini, B. G. Ribeiro
Principal component analysis (PCA) is a statistical technique used to identify variations in multivariate data obtained during the performance of the maximum ergospirometry test (MET). To use the PCA to compare the coefficients of change of the principal component (PC1) using the eigenvalue and the maximum values of the cardiorespiratory variables obtained in the athletes' in MET. 10 soccer players and 10 basketball players, all male, were evaluated. The PCA analyzed the values of the variables during the performance of the MET. The PC1 for each variable was calculated, and the eigenvalue was generated, representing the coefficients of variation of the PC1 of all variables. In the quantitative assessment (maximum values), a higher VO2max (3.93±0.62 vs. 3.41±0.37 l·min-1) was observed in basketball players compared to soccer players (p<0.05). The qualitative evaluation using PC1 of cardiorespiratory parameters (heart rate, minute volume, O2 consumption, CO2 production, expired fraction of O2 and expired fraction CO2) was observed as an eigenvalue (6.50±0.27 vs. 6.22±0.19) high for basketball players compared to soccer players (p<0.05). It is concluded that the basketball players showed more significant variability in their cardiorespiratory variables during the performance of the MET and higher VO2max at the end of the MET. These findings indicate that basketball players were less efficient in buffering the ventilatory acidosis observed during the MET. The results of this study highlight the importance of making complex assessments of the cardiorespiratory system, providing qualitative information to complement the quantitative data.
{"title":"Comparative Analysis of Cardiorespiratory Parameters of Basketball and Soccer Players Using Principal Component Analysis","authors":"Felipe Sampaio-Jorge, Anderson P Morales, Alexandre Miranda Rodrigues, M. Magini, B. G. Ribeiro","doi":"10.4028/p-840n5x","DOIUrl":"https://doi.org/10.4028/p-840n5x","url":null,"abstract":"Principal component analysis (PCA) is a statistical technique used to identify variations in multivariate data obtained during the performance of the maximum ergospirometry test (MET). To use the PCA to compare the coefficients of change of the principal component (PC1) using the eigenvalue and the maximum values of the cardiorespiratory variables obtained in the athletes' in MET. 10 soccer players and 10 basketball players, all male, were evaluated. The PCA analyzed the values of the variables during the performance of the MET. The PC1 for each variable was calculated, and the eigenvalue was generated, representing the coefficients of variation of the PC1 of all variables. In the quantitative assessment (maximum values), a higher VO2max (3.93±0.62 vs. 3.41±0.37 l·min-1) was observed in basketball players compared to soccer players (p<0.05). The qualitative evaluation using PC1 of cardiorespiratory parameters (heart rate, minute volume, O2 consumption, CO2 production, expired fraction of O2 and expired fraction CO2) was observed as an eigenvalue (6.50±0.27 vs. 6.22±0.19) high for basketball players compared to soccer players (p<0.05). It is concluded that the basketball players showed more significant variability in their cardiorespiratory variables during the performance of the MET and higher VO2max at the end of the MET. These findings indicate that basketball players were less efficient in buffering the ventilatory acidosis observed during the MET. The results of this study highlight the importance of making complex assessments of the cardiorespiratory system, providing qualitative information to complement the quantitative data.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"189 - 198"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44269466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, the technology of dental implants has advanced a lot and this has become one of the main reasons for dentists to refer to implants immediately after tooth extraction. Evaluation of cell survival in implantology studies to determine cell sensitivity determines the outcome of treatment. This study aimed to investigate the bone integration properties as well as the cytotoxicity between the implant surface and the jaw bone. In this study, cytotoxicity test was used to evaluate the toxicity and viability of sandblasted large grit acid etched, and sandblasted large grit acid etched active surfaces in 3A brand dental implants with G292 osteoblastic cells and human gingival fibroblasts cells are discussed. This operation was performed using a laboratory incubator of the German company MEMMERT for 24 hours, by neubauer lam cells counting for one hundred thousand cultured cells in each test at a temperature of 37 °C, a pressure of 1 atmosphere and 90% humidity. Based on the scanning electron microscopy images and the cytotoxicity test results, it can be seen that the bone graft of the implant, with the sandblasted large grit acid etched active surface treatment, is much better and also one week faster than the implant with the sandblasted large grit acid etched one. However, the viability of the implant with the sandblasted large grit acid etched active surface treatment for both G292 osteoblastic cells and human gingival fibroblasts cells samples was equal to 98.4% and 97.3%, respectively, and is lower than the sandblasted large grit acid etched surface treatment. The results show that the viability of the sandblasted large grit acid etched implant is about 1.5 to 2% higher than the sandblasted large grit acid etched active one, but the surface integrity of sandblasted large grit acid etched active is better than sandblasted large grit acid etched in all samples, and the treatment process is reduced by one week.
{"title":"Evaluation of Viability and Cell Proliferation in Bone and Gingival on Dental Implant Fixtures with Active Sandblasted and Sandblasted Surfaces by the Cytotoxicity Test Method","authors":"B. Mohammadi, E. Anbarzadeh","doi":"10.4028/p-gmmc8m","DOIUrl":"https://doi.org/10.4028/p-gmmc8m","url":null,"abstract":"In recent years, the technology of dental implants has advanced a lot and this has become one of the main reasons for dentists to refer to implants immediately after tooth extraction. Evaluation of cell survival in implantology studies to determine cell sensitivity determines the outcome of treatment. This study aimed to investigate the bone integration properties as well as the cytotoxicity between the implant surface and the jaw bone. In this study, cytotoxicity test was used to evaluate the toxicity and viability of sandblasted large grit acid etched, and sandblasted large grit acid etched active surfaces in 3A brand dental implants with G292 osteoblastic cells and human gingival fibroblasts cells are discussed. This operation was performed using a laboratory incubator of the German company MEMMERT for 24 hours, by neubauer lam cells counting for one hundred thousand cultured cells in each test at a temperature of 37 °C, a pressure of 1 atmosphere and 90% humidity. Based on the scanning electron microscopy images and the cytotoxicity test results, it can be seen that the bone graft of the implant, with the sandblasted large grit acid etched active surface treatment, is much better and also one week faster than the implant with the sandblasted large grit acid etched one. However, the viability of the implant with the sandblasted large grit acid etched active surface treatment for both G292 osteoblastic cells and human gingival fibroblasts cells samples was equal to 98.4% and 97.3%, respectively, and is lower than the sandblasted large grit acid etched surface treatment. The results show that the viability of the sandblasted large grit acid etched implant is about 1.5 to 2% higher than the sandblasted large grit acid etched active one, but the surface integrity of sandblasted large grit acid etched active is better than sandblasted large grit acid etched in all samples, and the treatment process is reduced by one week.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"165 - 172"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46315760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wanzhong Li, Haozhe Ma, Pei Dong, Chen Lin Liang, Long Li, Xi Feng Zhang
Nanomedicine has been used in tumor treatment and research due to its advantages of targeting, controlled release and high absorption rate. Silver nanoparticle (AgNPs), with the advantages of small particle size, and large specific surface area, are of great potential value in suppressing and killing cancer cells. Methods: AgNPs–polyethyleneimine (PEI) –folate (FA) (AgNPs–PF) were synthesised and characterised by several analytical techniques. The ovarian cancer cell line Skov3 was used as the cell model to detect the tumor treatment activity of AgNPs, AgNPs–PF and AgNPs+ AgNPs–PF. Results: Results shown that AgNPs–PF were successfully constructed with uniform particle size of 50–70 nm. AgNPs, AgNPs–PF, AgNPs–PF+ AgNPs all showed a certain ability to inhibit cancer cell proliferation, increase reactive oxygen species and decrease the mitochondrial membrane potential. All AgNPs, AgNPs–PF, AgNPs+ AgNPs–PF promoted DNA damage in Skov3 cells, accompanied by the generation of histone RAD51 and γ-H2AX site, and eventually leading to the apoptosis of Skov3 cells. The combination of AgNPs–PF and AgNPs had a more pronounced effect than either material alone. Conclusion: This study is to report that the combination of AgNPs+ AgNPs–PF can cause stronger cytotoxicity and induce significantly greater cell death compared to AgNPs or AgNPs–PF alone in Skov3 cells. Therefore, the combined application of drugs could be the best way to cancer treatment.
{"title":"Construction of a Silver Nanoparticle Complex and its Application in Cancer Treatment","authors":"Wanzhong Li, Haozhe Ma, Pei Dong, Chen Lin Liang, Long Li, Xi Feng Zhang","doi":"10.4028/p-s8bc3p","DOIUrl":"https://doi.org/10.4028/p-s8bc3p","url":null,"abstract":"Nanomedicine has been used in tumor treatment and research due to its advantages of targeting, controlled release and high absorption rate. Silver nanoparticle (AgNPs), with the advantages of small particle size, and large specific surface area, are of great potential value in suppressing and killing cancer cells. Methods: AgNPs–polyethyleneimine (PEI) –folate (FA) (AgNPs–PF) were synthesised and characterised by several analytical techniques. The ovarian cancer cell line Skov3 was used as the cell model to detect the tumor treatment activity of AgNPs, AgNPs–PF and AgNPs+ AgNPs–PF. Results: Results shown that AgNPs–PF were successfully constructed with uniform particle size of 50–70 nm. AgNPs, AgNPs–PF, AgNPs–PF+ AgNPs all showed a certain ability to inhibit cancer cell proliferation, increase reactive oxygen species and decrease the mitochondrial membrane potential. All AgNPs, AgNPs–PF, AgNPs+ AgNPs–PF promoted DNA damage in Skov3 cells, accompanied by the generation of histone RAD51 and γ-H2AX site, and eventually leading to the apoptosis of Skov3 cells. The combination of AgNPs–PF and AgNPs had a more pronounced effect than either material alone. Conclusion: This study is to report that the combination of AgNPs+ AgNPs–PF can cause stronger cytotoxicity and induce significantly greater cell death compared to AgNPs or AgNPs–PF alone in Skov3 cells. Therefore, the combined application of drugs could be the best way to cancer treatment.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"1 - 16"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44159954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A rapid and effective diagnostic method is essential for lung cancer since it shows symptoms only at its advanced stage. Research is being carried out in the area of exhaled breath analysis for the diagnosis of various pulmonary diseases including lung cancer. In this method exhaled breath volatile organic compounds (VOC) are analyzed with various techniques such as gas chromatography-mass spectrometry, ion mobility spectrometry, and electronic noses. The VOC analysis is suitable for lung cancer detection since it is non-invasive, fast, and also a low-cost method. In addition, this technique can detect primary stage nodules. This paper presents a systematic review of the various method employed by researchers in the breath analysis field. The articles were selected through various search engines like EMBASE, Google Scholar, Pubmed, and Google. In the initial screening process, 214 research papers were selected using various inclusion and exclusion criteria and finally, 55 articles were selected for the review. The results of the reviewed studies show that detection of lung cancer can be effectively done using the VOC analysis of exhaled breath. The results also show that this method can be used for detecting the different stages and histology of lung cancer. The exhaled breath VOC analysis technique will be popular in the future, bypassing the existing imaging techniques. This systematic review conveys the recent research opportunities, obstacles, difficulties, motivations, and suggestions associated with the breath analysis method for lung cancer detection.
{"title":"Exhaled Breath Volatile Organic Compound Analysis for the Detection of Lung Cancer- A Systematic Review","authors":"V. A. Binson, M. Subramoniam","doi":"10.4028/p-dab04j","DOIUrl":"https://doi.org/10.4028/p-dab04j","url":null,"abstract":"A rapid and effective diagnostic method is essential for lung cancer since it shows symptoms only at its advanced stage. Research is being carried out in the area of exhaled breath analysis for the diagnosis of various pulmonary diseases including lung cancer. In this method exhaled breath volatile organic compounds (VOC) are analyzed with various techniques such as gas chromatography-mass spectrometry, ion mobility spectrometry, and electronic noses. The VOC analysis is suitable for lung cancer detection since it is non-invasive, fast, and also a low-cost method. In addition, this technique can detect primary stage nodules. This paper presents a systematic review of the various method employed by researchers in the breath analysis field. The articles were selected through various search engines like EMBASE, Google Scholar, Pubmed, and Google. In the initial screening process, 214 research papers were selected using various inclusion and exclusion criteria and finally, 55 articles were selected for the review. The results of the reviewed studies show that detection of lung cancer can be effectively done using the VOC analysis of exhaled breath. The results also show that this method can be used for detecting the different stages and histology of lung cancer. The exhaled breath VOC analysis technique will be popular in the future, bypassing the existing imaging techniques. This systematic review conveys the recent research opportunities, obstacles, difficulties, motivations, and suggestions associated with the breath analysis method for lung cancer detection.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"17 - 35"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70641658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polyhydroxyalkanoates (PHA) are the biomaterials isolated naturally from bacterial strains. These are present in granules and accumulated intracellularly for storage and energy uptake in stressed conditions. This work was based on the extraction of polyhydroxyalkanoates from haloarchaeal strains isolated from samples of a salt mine and Halocin activity screening of these isolates. For the screening of polyhydroxyalkanoates, Nile Blue and Sudan Black Staining were performed. After confirmation and theoretical determination, polyhydroxyalkanoates extraction was done by sodium hypochlorite digestion and solvent extraction by chloroform method in combination. Polyhydroxyalkanoates production was calculated along with the determination of biomass. Halocin activity of these strains was also screened at different intervals. Isolated strains were identified by 16S RNA gene sequencing. Polyhydroxyalkanoates polymer was produced in form of biofilms and brittle crystals. Halocin activity was exhibited by four strains, among which confirmed halocin activity was shown by strain K7. The remarkable results showed that polyhydroxyalkanoates can replace synthetic plastics which are not environment friendly as they cause environmental pollution – a major threat to Earth rising gradually. Therefore, by switching to the use of biodegradable bioplastics from the use of synthetic plastics, it would be beneficial to the ecosphere.
{"title":"Scale-Up Studies for Polyhydroxyalkanoate and Halocin Production by Halomonas Sp. as Potential Biomedical Materials","authors":"K. Nasir, R. Batool, N. Jamil","doi":"10.4028/p-yqf2wv","DOIUrl":"https://doi.org/10.4028/p-yqf2wv","url":null,"abstract":"Polyhydroxyalkanoates (PHA) are the biomaterials isolated naturally from bacterial strains. These are present in granules and accumulated intracellularly for storage and energy uptake in stressed conditions. This work was based on the extraction of polyhydroxyalkanoates from haloarchaeal strains isolated from samples of a salt mine and Halocin activity screening of these isolates. For the screening of polyhydroxyalkanoates, Nile Blue and Sudan Black Staining were performed. After confirmation and theoretical determination, polyhydroxyalkanoates extraction was done by sodium hypochlorite digestion and solvent extraction by chloroform method in combination. Polyhydroxyalkanoates production was calculated along with the determination of biomass. Halocin activity of these strains was also screened at different intervals. Isolated strains were identified by 16S RNA gene sequencing. Polyhydroxyalkanoates polymer was produced in form of biofilms and brittle crystals. Halocin activity was exhibited by four strains, among which confirmed halocin activity was shown by strain K7. The remarkable results showed that polyhydroxyalkanoates can replace synthetic plastics which are not environment friendly as they cause environmental pollution – a major threat to Earth rising gradually. Therefore, by switching to the use of biodegradable bioplastics from the use of synthetic plastics, it would be beneficial to the ecosphere.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"49 - 60"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43733847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanotechnology, a fast-developing branch of science, is gaining extensive popularity among researchers simply because of the multitude of applications it can offer. In recent years, biological synthesis has been widely used instead of physical and chemical synthesis methods, which often produce toxic products. These synthesis methods are now being commonly adapted to discover new applications of nanoparticles synthesized using plant extracts. In this review, we elucidate the various ways by which nanoparticles can be biologically synthesized. We further discuss the applications of these nanoparticles.
{"title":"Methods for Green Synthesis of Metallic Nanoparticles Using Plant Extracts and their Biological Applications - A Review","authors":"Ravindran Krishnasamy, J. Obbineni","doi":"10.4028/p-8bf786","DOIUrl":"https://doi.org/10.4028/p-8bf786","url":null,"abstract":"Nanotechnology, a fast-developing branch of science, is gaining extensive popularity among researchers simply because of the multitude of applications it can offer. In recent years, biological synthesis has been widely used instead of physical and chemical synthesis methods, which often produce toxic products. These synthesis methods are now being commonly adapted to discover new applications of nanoparticles synthesized using plant extracts. In this review, we elucidate the various ways by which nanoparticles can be biologically synthesized. We further discuss the applications of these nanoparticles.","PeriodicalId":15161,"journal":{"name":"Journal of Biomimetics, Biomaterials and Biomedical Engineering","volume":"56 1","pages":"75 - 151"},"PeriodicalIF":0.7,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41318410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：