Journal of Asian Natural Products Research最新文献

Four new limonoids (1-4), reticulactone A (1), 21,23-dihydro-23-ethoxy-21-oxolimonin (2), tetrahydro-20,22-dihydroxy-21,23-diethoxy-nomilin (3), and dasycarinone B (4), as well as 11 known limonoids and one known carbolic acid, were isolated from the EtOH extract of the seeds of Citrus reticulata Blanco. The inhibitory effects of 15 limonoids (1-15) on the proliferation of HepG2 liver cancer cells were evaluated using the CCK-8 assay. Based on their structural characteristics, the results revealed that the typical limonoid compounds 2, 5, 10, and 12 exhibited stronger cytotoxicity compared to other limonoid compounds. Notably, the novel compound 2 demonstrated the strongest cytotoxic activity (IC₅₀ = 18.24 ± 2.78 μM).

A new isoflavone-quinone, placovinone E (1), and a new natural isoflavone, placovinone F (2), together with four known isoflavones (3-6), were isolated from the leaves of P. vietnamense. The chemical structures of all isolated compounds were elucidated using spectroscopic techniques (NMR and HRESIMS) and confirmed by comparison with literature data. All isolated compounds were evaluated for their α-glucosidase and xanthine oxidase inhibitory activities. Among them, compound 1 exhibited the strongest α-glucosidase inhibition (IC₅₀ 21.6 ± 1.04 µM), which was markedly more potent than the positive control, acarbose (IC₅₀ 156.2 ± 5.43 µM). In addition, compound 1 showed the highest xanthine oxidase inhibitory activity (IC₅₀ 32.8 ± 0.08 µM), although it remained considerably less potent than the positive control, allopurinol (IC₅₀ 2.45 ± 0.01 µM).

Clinacanthus nutans (CN) is traditionally used in medicine, but its apoptotic effects on breast cancer cells remain unclear. This study investigated the apoptotic potential of CN dichloromethane leaf extract (CNDCM) in MCF-7 cells using RNA-Seq analysis. Differential gene expression analysis identified 843 genes, with 395 upregulated and 448 downregulated. Gene ontology and KEGG pathway analyses revealed apoptosis-related pathways. Key apoptosis-associated genes, including ALDH3A1, CYP1A1, NOS3, MT1X, and CES1, were further validated by RT-qPCR. These findings suggest CNDCM as a potential candidate for breast cancer therapy.

One new compound daldiniaone A (1), along with six known compounds 1-(2,6-dihydroxyphenyl)ethan-1-one (2), 1-(2,6-dihydroxyphenyl)butan-1-one (3), penicilliode B (4), (4-hydroxy-3-methoxyphenyl)acetic acid (5), 2-(4-methoxyphenyl)acetic acid (6), and 2-(2-hydroxyethyl)phenol (7), were obtained from the EtOAc extract of the mangrove-derived fungus Daldinia eschscholtzii TGM23. Their structures were characterized by comprehensive spectral analysis, including nuclear magnetic resonance (NMR), high resolution electrospray ionization mass spectroscopy (HR-ESI-MS), and electronic circular dichroism (ECD) spectra. All the isolated compounds were tested for their inhibitory activity against three plant pathogenic fungi. Compound 6 showed inhibitory activity against Phytophthora capsici, with the EC₅₀ value of 106.5 μg/ml. Compound 7 exhibited inhibitory activity against Botrytis cinerea with the EC₅₀ value of 86.8 μg/ml.

This study developed an integrated ultra-high performance liquid chromatography triple quadrupole linear ion trap tandem mass spectrometry/tandem mass spectrometry (UHPLC-QTRAP-MS/MS) with gas chromatography-mass spectrometry (GC-MS) strategy to quantify 19 constituents of Eupatorii Herba (EH) in rat plasma. The validated method demonstrated acceptable precision (RSD < 13.86%), accuracy (-12.05 to 11.81%), extraction recovery (83.25-116.50%), matrix effect (85.14-115.36%; RSD < 14.10%), and stability (RSD < 13.26%). Key pharmacokinetic findings revealed rapid absorption for all detected analytes, with melilotoside, o-acetyl-p-cresol, and thymol exhibiting the highest systemic exposure. This work provided a validated analytical foundation for further pharmacological research on EH.

A chemical investigation on 80% ethanol extract from the whole plants of Tylophora ovata afforded two new C₂₁ steroidal glycosides, ovatacosides A and B (1 and 2). Structural elucidation of both compounds were performed by spectral methods such as 1D and 2D (¹H-¹H COSY, HMQC, and HMBC) NMR spectroscopy and high resolution mass spectrometry. The isolated components were evaluated in vitro for anti-inflammatory activities against COX-1 and COX-2 and radical scavenging potential using DPPH test. Consequently, 1 exhibited modest selective inhibition on COX-1 (61.9%) at the concentration of 100 μM and significant DPPH scavenging activity (IC₅₀ = 42.9 μM) comparable with positive control Trolox (IC₅₀ = 44.2 μM).

Triple-negative breast cancer (TNBC) is a type of breast cancer that has a high rate of growth and metastasis. In this study, the liquid fermentation product (EX-9) of Penicillium griseofulvum, an endophytic fungus derived from the medicinal plant Delphinium grandiflorum L., has exhibited significant anti-cancer activity against TNBC. We investigated the therapeutic effects and potential mechanisms of EX-9 on TNBC both in vivo and in vitro. Our findings demonstrated that EX-9 exhibited remarkable efficacy against TNBC both in vivo and in vitro, while showing negligible toxicity. The potential mechanism for its anti-TNBC effect involved the inhibition of the PI3K/Akt signaling pathway. Additionally, we employed liquid chromatography-mass spectrometry (LC-MS) technology and network pharmacology methods to uncover the potential pharmacological substance basis of EX-9 in combating TNBC.

Broussonetia papyrifera leaves are a medicinal plant rich in bioactive substances. This study utilized microbial fermentation of B. papyrifera leaf extract (BLE) to screen out Lactobacillus rhamnosus and Bifidobacterium papyrifera as the most suitable fermentation strains. The total phenols, total flavonoids, total tannins and total triterpenoids content increased after fermentation. The fermentation broth of L. rhamnosus and BLE (BLFs) could inhibit the tyrosinase, melanin production and related protein expression of mouse melanoma cell B16-F10. The fermentation broth of B. bifidum and BLE (BBFs) exhibited significant antioxidant effects. It indicates that the fermented BLE has great application potential in the fields of medicine, cosmetics and related areas.

One new nor-28-oleanane glycoside (1) and fifteen known compounds (2 - 16) were separated from a methanol extract of Syzygium formosum leaves. Their structures were elucidated via HRESIMS and NMR spectroscopic analyses. The absolute configuration of 1 was determined based on ECD spectra calculated using TD-DFT methods. Among the isolates, syringin (11) exhibited significant inhibitory activity against NO production in LPS-stimulated RAW264.7 cells, with an IC₅₀ value of 18.4 μM. Compounds 1 - 3 displayed moderate inhibitory effects, with IC₅₀ values of 58.4-79.2 μM.