Journal of Asian Natural Products Research最新文献

We studied the protective effects of Pandanus tectorius fruits (PTF) on Schwann cells and sciatic nerve damage in diabetic peripheral neuropathy (DPN) rats. PTF improved RSC96 cell viability and increased Nrf2, Keap1, HO-1, and NQO1 expression. PTF reduced apoptosis and ROS in high glucose-treated cells, which was reversed by Nrf2 siRNA. Co-treatment with SFN further decreased apoptosis and ROS. PTF increased SOD, GSH-Px, NGF, IGF-1, and VEGF activities, reduced by Nrf2 knockdown but restored with SFN. MDA levels showed opposite trends. In DPN rats, PTF reduced pain and prevented nerve damage, suggesting it alleviates DPN by activating the Nrf2/Keap1 pathway.

A comprehensive study was carried out on Penicillium brocae G2131, which originated from Yunnan Menghai Pu'er tea. This study resulted in the identification of five compounds, one of which is a newly discovered compound: (5aR)-6-((2R,5R,E)-5,6-dimethylhept-3-en-2-yl)-5a-methyl-5,5a,6,7,8,8a-hexa hydro-2H-indeno[5,4-b]furan-2-one (1), four known compounds: demethylincisterol A₃ (2), (22E, 24 R)-ergosta-7,9(11),22-trien-3β-ol (3), (22E,24R)-5α,8α-epidixyer-gosta-6,22-dien-3β-ol (4) and p- hydroxybenzaldehyde (5). The structure of compound 1 was determined using a variety of spectroscopic methods such as ¹H spectrum,¹³C spectrum, HMBC, HSQC,¹H-¹H COSY, LC-MS, UV, IR and comparison of their NMR data with literature. The absolute configuration of compound 1 was established by comparing the experimental and ECD spectra. All compounds were tested for their anti-acetylcholine activity, however, none of them demonstrated any anti-acetylcholine activity.

The 3-O-(4'-imidazole)-12-en-olean-28-amide derivatives 7-1 to 7-11 through modification the C-3 and C-28 of the natural product oleanolic acid were prepared, and their structures were confirmed by MS,¹H NMR and ¹³C NMR. The antitumor activities of these compounds against breast cancer MCF-7 and gastric cancer SGC7901 cells in vitro were determined by MTT assay. Cell tests showed that the antitumor activities of compound 7-10 exhibited significant antitumor activity which was equivalent to the positive control drug nilotinib, and the affinity was verified by molecular dynamics experiment in vitro. Molecular docking showed that compound 7-10 have high binding ability with c-kit. Therefore, compound 7-10 has the potential to become a new c-kit inhibitor, which deserves further research.

Lignosus rhinocerotis is rich in polysaccharide with diverse -bioactivities. This study developed a pre-column derivatization reversed-phase high-performance liquid chromatography (RP-HPLC) method for analyzing monosaccharides in Lignosus rhinocerotis polysaccharides (LRP). LRP underwent hydrolysis, derivatization, and separation on a Cosmosil 5C18-MS-II column at 254 nm. Baseline separation of eight standard monosaccharides was achieved within 45 min. Calibration curves, precision, and accuracy were validated. Quantitative analysis revealed LRP as a heteropolysaccharide containing mannose, ribose, rhamnose, glucose, galactose, xylose, and arabinose, with 100.28-111.02% recovery. This optimized RP-HPLC offers a simple, reproducible, and accurate tool for LRP monosaccharides analysis, facilitating in understanding its structure-function relationship.

This study aimed to assess the composition of Elsholtzia densa essential oil (EBE) and identify potential targets for inhibiting human hepatocellular carcinoma cell proliferation. The plants were collected from four regions: Jiuzhi, Qinghai; Ruoergai, Sichuan; Aba, Sichuan; and Jiulong, Sichuan. Four EBEs (named No. 1 to No. 4) were analyzed by gas chromatograph-mass spectrometer. EBEs significantly inhibited human hepatocellular carcinoma cells. The EBE collected from Jiuzhi exhibited the most potent inhibitory effect. Core targets identified included MAPK3, EGFR, ESR1, CASP3, PTGS2, BCL2L1, and MAPK14. Notably, the four EBEs prevented hepatocellular carcinoma cell proliferation via neuroactive ligand-receptor interactions and apoptosis pathways.

Angoroside C (AgrC) is a compound with many pharmacological properties. However, its antitumour potential has not been well studied. The low bioavailability of AgrC suggests a strong link to gut bacteria. Therefore, we identified and quantified four AgrC metabolites in gut microbiota. Molecular docking and inhibitor-based experiments demonstrated that carboxylesterase played a key role in AgrC metabolism. Both AgrC and its metabolites inhibited the viability of CT-26 cells, and potential antitumour targets were further explored. Additionally, AgrC significantly increased the levels of propionic, butyric, valeric and isovaleric acids. This provides a new insight for the antitumour effects of AgrC.

A series of C₂₁ steroidal glycosides were isolated from the roots and rhizomes of Vincetoxicum atratum (Bunge) C. Morren et Decne., including a new compound, cynatroside B₂ (1), and seven known compounds (2-8). Their structures were identified by comprehensive spectroscopic analyses, including NMR and HR-ESI-MS spectral data. The isolated steroids were evaluated for their anti-inflammatory activity against lipopolysaccharide-induced mouse macrophage RAW264.7 cells. Compound 1 displayed a significant inhibitory effect on NO, TNF-α and IL-1β.