Journal of Biomimetics, Biomaterials and Tissue Engineering最新文献

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Physico-Chemical Properties and Characterization of CaO-Fe2O3-P2O5 Glass as a Bioactive Ceramic Material 生物活性陶瓷材料CaO-Fe2O3-P2O5玻璃的理化性质及表征

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2012-01-31 DOI: 10.4028/www.scientific.net/JBBTE.12.1

M. R. Majhi, R. Kumar, S.P. Singh, R. Pyare

The Aim of this Work Is to Investigate Role of Iron in Calcium-Iron-Phosphate Bioglass. the Density, Compressive Strength, Tg Point and Leachability of Cations Were Measured. the Ph Behaviour of Simulated Body Fluid after Soaking Phosphate Glasses for Different Time Periods Were Also Studied and it Was Observed that Higher Phosphate Glasses Containing Lower Lime Possessed Better Bioactivity than Lower Phosphate Glasses Containing More Iron and Lime Contents. the DTA and FTIR Spectrometry of Glasses Were Performed. the Absorption Spectra Showed that Iron Was Present in the Glass only as Fe3+ Ion. the Leachability of Ca2+ and Fe3+ Ions from Glass Was due to Diffusion Control and P5+ Ion due to Network Break down of PO4 Tetrahedra. the DTA Peaks Were Broad. the FTIR Band around 1000 Cm-1 in Glass Was due to Asymmetric Stretching of O=P=O Linkage. the FTIR Absorption and Reflectance Spectrometry of the Glass Samples after SBF Treatment Had Confirmed the Deposition of Bone-Like Hydroxyl Carbonate Apatite Layer on the Glass Surfaces for their Bioactivity. the Bands Centred in between 2880-3425 Cm-1 Were due to Presence of OH Groups and Stretching Modes of H-O-H Vibration in Glasses. the Properties and Structure Relationship for Glasses Were Established and the Results Were Discussed.

本文旨在探讨铁在磷酸铁钙生物玻璃中的作用。测定了阳离子的密度、抗压强度、Tg点和浸出率。研究了磷酸盐玻璃浸泡不同时间后模拟体液的Ph值变化，发现含少量石灰的高磷酸盐玻璃比含较多铁和石灰的低磷酸盐玻璃具有更好的生物活性。对玻璃进行了DTA和FTIR光谱分析。吸收光谱表明，铁在玻璃中仅以Fe3+离子的形式存在。Ca2+和Fe3+离子的可浸出性主要是由于扩散控制，P5+离子的可浸出性主要是由于PO4四面体的网络破坏。DTA峰宽。在1000 Cm-1附近的FTIR波段是由于O=P=O键的不对称拉伸。经SBF处理后的玻璃样品的FTIR吸收和反射光谱分析证实，在玻璃表面沉积了骨状羟基碳酸盐磷灰石层，具有生物活性。以2880 ~ 3425 Cm-1为中心的谱带是由于OH基团的存在和H-O-H振动的拉伸模式造成的。建立了玻璃的性能和结构关系，并对结果进行了讨论。

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引用次数: 6

Synthesis and Characterization of Sol-Gel Derived Hydroxyapatite-Bioglass Composite Nanopowders for Biomedical Applications 生物医学用溶胶-凝胶羟基磷灰石-生物玻璃复合纳米粉体的合成与表征

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2012-01-31 DOI: 10.4028/www.scientific.net/JBBTE.12.51

S. Adibnia, A. Nemati, M. Fathi, S. Baghshahi

The main purpose of this study is to prepare and characterize hydroxyapatite (HA)–10%wt bioglass (BG) composite nanopowders and its bioactivity. Composites of hydroxyapatite with synthesized bioglass are prepared at various temperatures. Suitable calcination temperature is chosen by evaluating of the phase composition. X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) techniques are utilized to characterize the prepared nanopowders. The bioactivity of the prepared composite samples is evaluated in an in vitro study by immersion of samples in simulated body fluid (SBF) for predicted time. Fourier transformed infrared (FTIR) spectroscopy and inductively coupled plasma (ICP) are used for evaluation of apatite formation and the bioactivity properties. Results show that HA-BG composite nanopowders are successfully prepared without any decomposition of hydroxyapatite. The suitable temperature for calcination is 600°C and the particle size of hydroxyapatite is about 40-70 nm. The apatite phase forms after 14 days immersing of the samples in SBF. It could be concluded that this process can be used to synthesize HA-BG composite nanopowders with improved bioactivity which is much needed for hard tissue repair and biomedical applications.

本研究的主要目的是制备和表征羟基磷灰石(HA) -10%wt生物玻璃(BG)复合纳米粉体及其生物活性。在不同温度下制备了羟基磷灰石与合成生物玻璃的复合材料。通过对相组成的评价，选择合适的煅烧温度。利用x射线衍射(XRD)、透射电子显微镜(TEM)和扫描电子显微镜(SEM)技术对制备的纳米粉体进行了表征。制备的复合样品的生物活性在体外研究中通过将样品浸泡在模拟体液(SBF)中预测时间来评估。傅里叶变换红外光谱(FTIR)和电感耦合等离子体(ICP)用于评价磷灰石的形成和生物活性特性。结果表明，制备的HA-BG复合纳米粉体没有羟基磷灰石的分解。煅烧的适宜温度为600℃，羟基磷灰石的粒径约为40 ~ 70 nm。样品在SBF中浸泡14天后形成磷灰石相。由此可见，该工艺可制备具有较高生物活性的HA-BG复合纳米粉体，在硬组织修复和生物医学应用中具有重要的应用价值。

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引用次数: 5

Effect of Biomimetic Nanocrystalline Apatite Surface on Bone Ingrowth 仿生纳米磷灰石表面对骨长入的影响

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2012-01-31 DOI: 10.4028/www.scientific.net/JBBTE.12.41

J. Gelber, J. Hermida, S. Patil, C. Colwell, D. D’Lima

The Success of Hydroxyapatite-Coated Acetabular Components Has Not Been Consistent. Plasma-Sprayed Hydroxyapatite Coatings Work Well on Nonporous Substrates but Do Not Coat the Inner Surfaces of Open-Porous Substrates. Solution Deposition Can Generate Consistent Bioceramic Coats on Porous Surfaces that More Closely Mimic the Trabecular Pattern and Biochemistry at the Bone Interface. we Compared Bone Response to the Following Implants: Porous-Coated Ti6al4v Cylinders with 1 of 3 Treatments: Plasma Sprayed with Hydroxyapatite (HA), Coated with a Solution-Deposited Biomimetic Apatite Coating (BA), and Untreated (Control). Bilateral Femurs in 36 Rabbits Were Implanted with One of the above Implants. Bone Ingrowth for HA and BA Surfaces Was Significantly Higher than that for Control Surfaces. No Fragmentation or Debris Production Was Evident in the Apatite Coat of the BA Group. A Biomimetic Coat of Solution-Deposited Apatite May Be Resistant to Coating Delamination and Particle Generation.

羟基磷灰石包覆的髋臼假体的成功并不是一致的。等离子喷涂羟基磷灰石涂层在无孔基底上效果良好，但在开放多孔基底的内表面不能涂覆。溶液沉积可以在多孔表面产生一致的生物陶瓷涂层，更接近地模拟骨界面的小梁模式和生物化学。我们比较了以下种植体的骨反应:三种处理中的一种:等离子喷涂羟基磷灰石(HA)、涂覆溶液沉积仿生磷灰石涂层(BA)和未处理(对照)。36只兔双侧股骨植入上述一种植入物。羟基磷灰石和羟基磷灰石表面的骨长入显著高于对照表面。BA组磷灰石层中没有明显的碎块或碎屑产生。溶液沉积磷灰石的仿生涂层可以抵抗涂层分层和颗粒生成。

引用次数: 0

Finite Element Modelling of Rheological Property of Curing PMMA Bone Cement. Part 1 - Effect of Prosthesis Insertion Velocity 固化PMMA骨水泥流变特性的有限元模拟。第一部分-假体插入速度的影响

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2012-01-31 DOI: 10.4028/www.scientific.net/JBBTE.12.83

M.M. Rahman, A. Olabi, M. Hashmi

PMMA (Polymethyl methacrylate) bone cement is currently the only material used for anchoring cemented arthroplasties to contiguous bones. The aim of this work is to model the flow of bone cement through porous cancellous bone to study the degree of penetration in total hip replacement using FIDAP simulation software. Two different viscosity models have been used (a) power law with constant consistency index and power law index less than 1 for pseudoplastic behaviour of Simplex P® and Zimmer bone cement; (b) FORTRAN subroutine for time dependent rheopectic behaviour of CMW3 and Zimmer bone cement. For each type of bone cement the effect of prosthesis insertion velocity have been investigated under four different values 5, 10, 15 and 20 mm/s. It has been observed that the depth of penetration increases with increasing prosthesis insertion velocity. On the other hand, the maximum pressure in bone cement decreases with increasing prosthesis insertion velocity. It has been observed that there is more penetration through cancellous bone for pseudoplastic behaviour than rheopectic behaviour of bone cement.

PMMA(聚甲基丙烯酸甲酯)骨水泥是目前唯一用于连续骨锚定骨水泥关节置换术的材料。本工作的目的是利用FIDAP仿真软件模拟骨水泥通过多孔松质骨的流动，研究全髋关节置换术中骨水泥的渗透程度。采用了两种不同的黏度模型(a)对Simplex P®和Zimmer骨水泥的假塑性行为进行了幂律分析，幂律指数为常数，幂律指数小于1;(b) CMW3和Zimmer骨水泥随时间变化流变行为的FORTRAN子程序。在5、10、15和20 mm/s 4种不同的数值下，研究了不同类型骨水泥对假体插入速度的影响。我们观察到插入深度随着假体插入速度的增加而增加。另一方面，骨水泥内的最大压力随假体插入速度的增加而减小。已经观察到骨水泥的假塑性行为比流变行为更能穿透松质骨。

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引用次数: 2

An Investigation of the Carriage Rate of Methicillin-Resistant Staphylococcus aureus in Pigs in the Western Province of Vietnam 越南西部省猪群耐甲氧西林金黄色葡萄球菌携带率调查

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2012-01-31 DOI: 10.4028/www.scientific.net/JBBTE.12.91

T. Vo, An Doan Mai Huynh, H. Ngo, D. T. Nguyen, Thi Minh Tam Tran

Methicillin-resistant Staphylococcus aureus (MRSA) transmission between pigs and humans can be a dangerous infection source for the community. To control and prevent such disease, MRSA research in healthy pigs has been conducted in the US and Holland. This paper reports the first such study in Vietnam. This study aims to optimize the method for investigating MRSA carriage in tonsil and nasal swabs of healthy pigs. Potential samples were selected rapidly by Multiplex PCR (M-PCR) assay with 3 primers (Staph 756, mecA, Sa442) and then combined with conventional methods to detect and pick up MRSA strains. These strains were grouped based on genome pattern by pulsed field gel electrophoresis (PFGE). This study showed that we successfully isolated MRSA and the rate of MRSA carriage samples was 3.63%, much lower than that reported in the USA (49%) and Holland (39%), however the USA and Holland studies investigated pigs from industrial scale piggeries, whereas our study investigated pigs from small farms, which is the usual approach to pig farming in Vietnam. The MRSA patterns identified and associated MRSA strains were different from identified MRSA strains in other countries. This research provides important information about the carriage rate of MRSA in pigs in the Western province of Vietnam and demonstrates the importance of ongoing investigations in this area.

猪与人之间的耐甲氧西林金黄色葡萄球菌(MRSA)传播可能是社区的危险感染源。为了控制和预防这种疾病，美国和荷兰已经在健康的猪身上进行了MRSA研究。本文报道了在越南进行的首次此类研究。本研究旨在优化研究健康猪扁桃体和鼻拭子携带MRSA的方法。采用3种引物(Staph 756、mecA、Sa442)进行多重PCR (M-PCR)快速筛选潜在样本，结合常规方法进行MRSA菌株的检测和分离。采用脉冲场凝胶电泳(PFGE)技术对菌株进行分组。本研究表明，我们成功分离了MRSA, MRSA携带样本的比率为3.63%，远低于美国(49%)和荷兰(39%)的报道，然而，美国和荷兰的研究调查了来自工业规模养猪场的猪，而我们的研究调查了来自小型养猪场的猪，这是越南养猪业的常用方法。所鉴定的MRSA模式和相关的MRSA菌株与其他国家鉴定的MRSA菌株不同。本研究提供了有关越南西部省猪中MRSA携带率的重要信息，并表明了在该地区进行持续调查的重要性。

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引用次数: 2

Osteocyte Characterization on Polydimethylsiloxane Substrates for Microsystems Applications. 用于微系统应用的聚二甲基硅氧烷基底上的骨细胞特征描述

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2012-01-01 DOI: 10.4028/www.scientific.net/JBBTE.16.27

Spencer L York, Ahmad R Arida, Karan S Shah, Palaniappan Sethu, Marnie M Saunders

In the body, osteocytes reside in lacunae, lenticular shaped cavities within mineralized bone. These cells are linked to each other and surface-residing osteoblasts via physical channels known as gap junctions. It has been suggested that osteocytes sense mechanical load applied to bone and relay that signal to osteoclasts and osteoblasts. Current in vitro and in vivo models of mechanotransduction face temporal and spatial barriers. Recent advances in polydimethylsiloxane (PDMS) based microfabrication techniques may be able to overcome some of these hurdles. However, before the bone research field can effectively utilize microsystems techniques, fundamental groundwork must be completed. This study characterized the behaviour of osteocytes on PDMS coated with collagen type I (CTI) and provides the framework for bone cell mechanotransduction studies using microsystems. The goal was to determine whether osteocytes were adversely affected by the substrate material by comparing their behaviour to a standard glass substrate. In addition, optimal culture conditions and time points for growing osteocytes on PDMS substrates were determined. Results of this study suggested that use of PDMS does not adversely affect osteocyte behaviour. Furthermore, the results demonstrated that osteocytes should be cultured for no less than 72 hours prior to experimentation to allow the establishment and maintenance of phenotypic characteristics. These results completed essential groundwork necessary for further studies regarding osteocytes in microsystems modelling utilizing PDMS.

在人体内，骨细胞居住在矿化骨内的透镜状腔隙中。这些细胞通过称为间隙连接的物理通道相互连接，并与表面驻留的成骨细胞相连。有人认为，骨细胞能感知施加在骨骼上的机械负荷，并将信号传递给破骨细胞和成骨细胞。目前的体外和体内机械传导模型面临时间和空间障碍。基于聚二甲基硅氧烷（PDMS）的微加工技术的最新进展或许能克服其中一些障碍。然而，在骨骼研究领域有效利用微系统技术之前，必须完成基本的基础工作。这项研究描述了涂有 I 型胶原（CTI）的 PDMS 上骨细胞的行为特征，并为利用微系统进行骨细胞机械传导研究提供了框架。目的是通过将骨细胞的行为与标准玻璃基底进行比较，确定骨细胞是否会受到基底材料的不利影响。此外，还确定了在 PDMS 基质上培养骨细胞的最佳培养条件和时间点。研究结果表明，使用 PDMS 不会对成骨细胞的行为产生不利影响。此外，研究结果表明，实验前应培养骨细胞不少于 72 小时，以便建立和维持表型特征。这些结果为进一步研究利用 PDMS 进行微系统建模的骨细胞奠定了必要的基础。

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引用次数: 0

Sequential Release of BMP-7 and VEGF from the PLGA/AK-Gelatin Composite Scaffolds BMP-7和VEGF在PLGA/ ak -明胶复合支架中的顺序释放

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2011-09-01 DOI: 10.4028/www.scientific.net/JBBTE.11.81

Gang Liu, W. Fan, X. Miao, Yin Xiao, David Good, M. Wei

Vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMP-7) are key regulators of angiogenesis and osteogenesis during bone regeneration. The aim of this study was to investigate the possibility of realizing sequential release of the two growth factors using a novel composite scaffold. Poly(lactic-co-glycolic acid) (PLGA)-Akermanite (AK) microspheres were used to make the composite scaffold, which was then loaded with BMP-7, followed by embedding in a gelatin hydrogel matrix loaded with VEGF. The release profiles of the growth factors were studied and selected osteogenic related markers of bone marrow stromal cells (BMSCs) were analysed. It was shown that the composite scaffolds exhibited a fast initial burst release of VEGF within the first 3 days and a sustained slow release of BMP-7 over the full period of 20 days. The in vitro proliferation and differentiation of the BMSCs cultured in the osteogenic medium were enhanced by 1 to 2 times, resulting from the additionally and sequentially release of growth factors from the PLGA-AK/gelatin composite scaffolds.

血管内皮生长因子(VEGF)和骨形态发生蛋白(BMP-7)是骨再生过程中血管生成和骨生成的关键调控因子。本研究的目的是探讨使用一种新型复合支架实现两种生长因子顺序释放的可能性。使用聚乳酸-羟基乙酸(PLGA)-Akermanite (AK)微球制作复合支架，然后负载BMP-7，然后包埋在负载VEGF的明胶水凝胶基质中。研究了生长因子的释放谱，分析了骨髓基质细胞成骨相关标志物。结果表明，复合支架在头3天内表现出快速的初始爆发性释放VEGF，在整个20天内表现出持续缓慢释放BMP-7。由于PLGA-AK/明胶复合支架中生长因子的附加和顺序释放，BMSCs在成骨培养基中培养的体外增殖和分化能力提高了1 ~ 2倍。

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引用次数: 2

A Method to Fabricate Liver Tissue Engineering Scaffold 一种制备肝组织工程支架的方法

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2011-09-01 DOI: 10.4028/www.scientific.net/JBBTE.11.73

S. Singare, S. Zhong, Z. Sun

In this paper, the authors describe a rapid prototyping method to produce vascularized tissue such as liver scaffold for tissue engineering applications. A scaffold with an interconnected channel was designed using a CAD environment. The data were transferred to a Polyjet 3D Printing machine (Eden 250, Object, Israel) to generate the models. Based on the 3D Printing model, a PDMS (polydimethyl-silicone) mould was created which can be used to cast the biodegradable material. The advantages and limitations of Rapid Prototyping (RP) techniques as well as the future direction of RP development in tissue engineering scaffold fabrication were reviewed.

在本文中，作者描述了一种快速成型的方法来生产血管化组织，如肝支架的组织工程应用。在CAD环境下，设计了具有连通通道的支架。数据被传输到Polyjet 3D打印机(Eden 250, Object, Israel)来生成模型。基于3D打印模型，创建了PDMS(聚二甲基硅树脂)模具，可用于铸造可生物降解材料。综述了快速成型技术在组织工程支架制造中的优势和局限性，并展望了快速成型技术在组织工程支架制造中的发展方向。

引用次数: 3

Preparation and Characterization of CaF2 Doped Bioglass Ceramics 掺CaF2生物玻璃陶瓷的制备与表征

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2011-09-01 DOI: 10.4028/www.scientific.net/JBBTE.11.45

M. R. Majhi, R. Pyare, S. Singh

Bioglass Ceramics having molar composition 40SiO2-(44-X)CaO-10MgO-6P2O5-XCaF2 (where X = 0 to 8%) were prepared by conventional melting process in an electric globar furnace at 1400±10°C. Controlled crystallizations were carried out to convert the bioglasses to their corresponding ceramics. Nucleation and crystallization regimes were carried out by differential thermal analysis. The crystalline phases termed hydroxy fluoroapatite, akermanite and wollastonite were identified by using x-ray diffraction analysis. The investigation of bioactivity for the prepared glass and glass ceramics was done by infrared absorption and infrared reflection spectra after immersion in simulated body fluid (SBF) for different periods at 37.8°C. Scanning electron microscope (SEM) analysis was carried out to investigate the surface texture. Micrographs show the formation of HCA layer on the surface of the bioglass ceramics samples after 7 days of SBF treatment. The surfaces of the samples were completely covered with irregular and needle-like aggregates of Ca–P layer. The released ions were estimated by atomic absorption spectroscopy. The chemical durability of these materials was determined by pH measurement methods and it was found that pH of the solution increases up from 1 to 7 days. Further, pH decreases with increasing time period, from 15 to 30 days in SBF solution.

在1400±10℃的电球炉中，采用常规熔融工艺制备了摩尔成分为40SiO2-(44-X)CaO-10MgO-6P2O5-XCaF2 (X = 0 ~ 8%)的生物玻璃陶瓷。通过控制结晶将生物玻璃转化为相应的陶瓷。通过差热分析进行了成核和结晶过程的研究。通过x射线衍射分析，确定了羟基氟磷灰石、铁长石和硅灰石的结晶相。制备的玻璃和玻璃陶瓷在37.8℃的模拟体液(SBF)中浸泡不同时间后，通过红外吸收和红外反射光谱研究其生物活性。采用扫描电镜(SEM)对其表面织构进行分析。显微照片显示SBF处理7天后生物玻璃陶瓷样品表面形成了HCA层。样品表面完全覆盖了不规则的针状Ca-P层聚集物。释放离子用原子吸收光谱法测定。通过pH测量方法测定了这些材料的化学耐久性，发现溶液的pH值从1天到7天增加。此外，pH值随时间的增加而降低，在SBF溶液中从15天到30天。

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引用次数: 3

Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering 人牙髓干细胞培养在组织工程中的应用研究

Journal of Biomimetics, Biomaterials and Tissue Engineering

Pub Date : 2011-09-01 DOI: 10.4028/www.scientific.net/JBBTE.11.13

Tran Le Bao Ha, Doan Nguyen Vu, T. Quan, Ngoc Phan Kim, Hung Hoang Tu, Bao Tram Hoang Dao, Ngoc Mai Dang Vu, T. Nguyen

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

牙髓细胞的研究在牙组织再生方面具有广阔的应用前景。本研究旨在建立人牙髓干细胞的体外培养方案，并将其应用于组织工程。收集人前磨牙和阻生第三磨牙并消毒。用Dulbecco's Modified Eagle培养基:营养混合物F-12 (DMEM/F12)培养基添加10%胎牛血清(FBS)培养牙髓碎片。采用增殖试验、RT-PCR和流式细胞术对牙髓干细胞进行鉴定。MTT法观察牙本质表面DPSCs的生长情况。研究表明，我们成功地分离、培养了牙髓细胞，并对其进行了表征。培养高水平表达Oct4、CD146、CD90、CD44的细胞群。DPSCs在化学处理和机械处理的牙本质表面增殖。本研究为进一步研究建立口腔组织工程规程提供了重要信息和基础。

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引用次数: 7

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