Pub Date : 2013-12-26DOI: 10.4172/1662-100X.1000E101
E. Kolos, A. Ruys
Tissue engineering offers a novel route for repairing damaged or diseased tissue by incorporating the patient’s own healthy cells or donated cells into temporary scaffolds that act as a matrix for cell cultivation. The structure and properties of these scaffolds must be selected to ensure normal cell behaviour and performance of the cultivated tissue. The tissue scaffolds support cellular growth and activity both before implantation and during remodelling of surrounding tissue after implantation.
{"title":"Biomimetic Scaffold Materials Used in Tissue Engineering","authors":"E. Kolos, A. Ruys","doi":"10.4172/1662-100X.1000E101","DOIUrl":"https://doi.org/10.4172/1662-100X.1000E101","url":null,"abstract":"Tissue engineering offers a novel route for repairing damaged or diseased tissue by incorporating the patient’s own healthy cells or donated cells into temporary scaffolds that act as a matrix for cell cultivation. The structure and properties of these scaffolds must be selected to ensure normal cell behaviour and performance of the cultivated tissue. The tissue scaffolds support cellular growth and activity both before implantation and during remodelling of surrounding tissue after implantation.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75410030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.21
S. R. Shah
Antiplatelet drugs block the formation of blood clots by preventing the clumping of platelets inside arteries, particularly in individuals who have atherosclerosis of their arteries, or are otherwise prone to develop blood clots in their arteries. Atherosclerosis is a slow disease in which arteries become clogged and hardened. Fat, cholesterol, calcium, and other substances form plaque, which builds up in arteries. Hard plaque narrows the passage that blood flows through. That causes arteries to become hard and inflexible. Soft plaque is more likely to break free from the artery wall and cause a blood clot, which can block blood flow to vital organs. In this paper an attempt has been made to investigate the effects of antiplatelet drugs on blood flow and significance of non-Newtonian viscosity through a stenosed artery using Herschel-Bulkely fluid model. Numerical illustration presented at the end of the paper provides the results for the resistance to flow, apparent viscosity and the wall shear stress through their graphical representations.
{"title":"Effects of Antiplatelet Drugs on Blood Flow through Stenosed Blood Vessels","authors":"S. R. Shah","doi":"10.4028/www.scientific.net/JBBTE.18.21","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.21","url":null,"abstract":"Antiplatelet drugs block the formation of blood clots by preventing the clumping of platelets inside arteries, particularly in individuals who have atherosclerosis of their arteries, or are otherwise prone to develop blood clots in their arteries. Atherosclerosis is a slow disease in which arteries become clogged and hardened. Fat, cholesterol, calcium, and other substances form plaque, which builds up in arteries. Hard plaque narrows the passage that blood flows through. That causes arteries to become hard and inflexible. Soft plaque is more likely to break free from the artery wall and cause a blood clot, which can block blood flow to vital organs. In this paper an attempt has been made to investigate the effects of antiplatelet drugs on blood flow and significance of non-Newtonian viscosity through a stenosed artery using Herschel-Bulkely fluid model. Numerical illustration presented at the end of the paper provides the results for the resistance to flow, apparent viscosity and the wall shear stress through their graphical representations.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"9 1","pages":"21 - 27"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80864531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.13
Shuwen Zhou, Siqi Zhang
A three-dimensional multi-body model of the 50th percentile male human and discretized neck was built to evaluate the effect of active head restraint on cervical vertebrae injuries lessening in vehicle rear impact. The discretized neck includes of cervical spine vertebrae, intervertebral discs, ligaments, and muscles. The BioRID-II adult male dummy restrained using safety belt was seated on a sled, whose longitudinal velocity measured from rear impact FEM simulation was applied to simulate the relative motion of the head and neck. According to the interspinous ligament loads and the ligamenta flava loads of the cervical spine, an active head restraint and an impact absorber were designed to lessening the neck injuries in vehicle rear end collisions.
{"title":"Effect of Active Head Restraint on Cervical Vertebrae Injuries Lessening","authors":"Shuwen Zhou, Siqi Zhang","doi":"10.4028/www.scientific.net/JBBTE.18.13","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.13","url":null,"abstract":"A three-dimensional multi-body model of the 50th percentile male human and discretized neck was built to evaluate the effect of active head restraint on cervical vertebrae injuries lessening in vehicle rear impact. The discretized neck includes of cervical spine vertebrae, intervertebral discs, ligaments, and muscles. The BioRID-II adult male dummy restrained using safety belt was seated on a sled, whose longitudinal velocity measured from rear impact FEM simulation was applied to simulate the relative motion of the head and neck. According to the interspinous ligament loads and the ligamenta flava loads of the cervical spine, an active head restraint and an impact absorber were designed to lessening the neck injuries in vehicle rear end collisions.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"76 1","pages":"13 - 20"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79282783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.43
M. Mahabole, Manjushree Bahir, R. Khairnar
Abstract: In this study, in-vitro bioactivity of manganese blended hydroxyapatite (Mn-HAp) pellets is carried out using simulated body fluid (SBF) solution. The incubated Mn-HAp samples are characterized by XRD, FTIR and SEM/EDAX. Dielectric and photoluminescence properties of Mn-HAp samples are studied as a function of incubation period in SBF. XRD profiles show that hexagonal apatite structure remains intact after partial replacement of calcium ions by manganese ions and even after incubation. The change in absorption due to phosphate group, depicted in FTIR spectra, for incubated samples confirms growth of apatite on Mn-HAp surface. SEM/ EDAX studies suggest that Mn-HAp surface promotes the growth of apatite without changing its structure due to apatite nucleation and growth on the surface of Mn-HAp. The value of dielectric constant of Mn-HAp increases after incubation. Increase in period of immersion in m-SBF leads to decrease in dielectric constant of manganese exchanged hydroxyapatite. The photoluminescence (PL) study reveals that the Mn-HAp can be used stable and efficient blue luminescent material.
{"title":"Mn Blended Hydroxyapatite Nanoceramic: Bioactivity, Dielectric and Luminescence Studies","authors":"M. Mahabole, Manjushree Bahir, R. Khairnar","doi":"10.4028/www.scientific.net/JBBTE.18.43","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.43","url":null,"abstract":"Abstract: In this study, in-vitro bioactivity of manganese blended hydroxyapatite (Mn-HAp) pellets is carried out using simulated body fluid (SBF) solution. The incubated Mn-HAp samples are characterized by XRD, FTIR and SEM/EDAX. Dielectric and photoluminescence properties of Mn-HAp samples are studied as a function of incubation period in SBF. XRD profiles show that hexagonal apatite structure remains intact after partial replacement of calcium ions by manganese ions and even after incubation. The change in absorption due to phosphate group, depicted in FTIR spectra, for incubated samples confirms growth of apatite on Mn-HAp surface. SEM/ EDAX studies suggest that Mn-HAp surface promotes the growth of apatite without changing its structure due to apatite nucleation and growth on the surface of Mn-HAp. The value of dielectric constant of Mn-HAp increases after incubation. Increase in period of immersion in m-SBF leads to decrease in dielectric constant of manganese exchanged hydroxyapatite. The photoluminescence (PL) study reveals that the Mn-HAp can be used stable and efficient blue luminescent material.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"50 1","pages":"43 - 59"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78128078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.85
Sandro Bittencourt Sousa, Igor Iuco Castro‐Silva, Lawrence Andrade Costa da Rocha Coutinho, Ariel Lenharo, J. Granjeiro
This clinical study evaluated the tissue repair process using different bone grafts. Nine dental patients with indication for posterior implantodontic treatment were submitted to maxillary sinus lifting procedures associated to grafting. After 6 months, bone biopsies (6 sites with allogenous bone grafting and 13 sites with bovine xenogenous bone grafting/OsseusTM) were removed and processed for histopathological and histomorphometric analyses (ANOVA and Tukey's test). Both groups had tissue biocompatibility without significant inflammatory response, only punctual presence of multinucleated giant cells in xenograft group. Osteoconductive potential was evidenced by new bone tissue surrounding and in direct contact with the granules of both grafts. Volume density of connective tissue was similar between groups, although there were significant differences in allograft group in comparison to xenograft as the presence of new bone formation (48.50%±13.93 versus 29.83±9.56, respectively, p<0.05) and remnant biomaterial (1.57±2.39 versus 22.23±12.41, respectively, p<0.001), suggesting a greater osteoconductivity and faster bioresorption in the allograft group. These results demonstrate that allogenous bone and OsseusTM can be satisfactorily used as grafts in minor oral surgeries for bone augmentation in humans.
{"title":"Osteoconduction and Bioresorption of Bone Allograft versus Anorganic Bovine Bone Xenograft: A Histomorphometric Study in Humans","authors":"Sandro Bittencourt Sousa, Igor Iuco Castro‐Silva, Lawrence Andrade Costa da Rocha Coutinho, Ariel Lenharo, J. Granjeiro","doi":"10.4028/www.scientific.net/JBBTE.18.85","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.85","url":null,"abstract":"This clinical study evaluated the tissue repair process using different bone grafts. Nine dental patients with indication for posterior implantodontic treatment were submitted to maxillary sinus lifting procedures associated to grafting. After 6 months, bone biopsies (6 sites with allogenous bone grafting and 13 sites with bovine xenogenous bone grafting/OsseusTM) were removed and processed for histopathological and histomorphometric analyses (ANOVA and Tukey's test). Both groups had tissue biocompatibility without significant inflammatory response, only punctual presence of multinucleated giant cells in xenograft group. Osteoconductive potential was evidenced by new bone tissue surrounding and in direct contact with the granules of both grafts. Volume density of connective tissue was similar between groups, although there were significant differences in allograft group in comparison to xenograft as the presence of new bone formation (48.50%±13.93 versus 29.83±9.56, respectively, p<0.05) and remnant biomaterial (1.57±2.39 versus 22.23±12.41, respectively, p<0.001), suggesting a greater osteoconductivity and faster bioresorption in the allograft group. These results demonstrate that allogenous bone and OsseusTM can be satisfactorily used as grafts in minor oral surgeries for bone augmentation in humans.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"45 1","pages":"85 - 95"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77440691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.97
F. Y. Zhou, A. Wei, A. Diwan
Intervertebral disc degeneration creates a significant healthcare burden on industrialized Western society. Recent research into treatment options has highlighted numerous biological strategies to replenish the native disc cells with minimal invasion, although their effectiveness and safety are still under intense investigation. This review provides an outline of the key biological therapies under research currently, including stem cells, biomaterial scaffolds and signaling molecules. Particularly, the focus will be on CDMP-2, a signaling molecule that has been found to possess properties that are therapeutic to disc degeneration. The review will assess the evidence of the molecule’s chondrogenic and chemotactic effects on disc cells and evaluate areas for further research in determining its regenerative potential in the intervertebral disc.
{"title":"An Overview of Intervertebral Disc Degeneration Therapies and an Evaluation of the Chondrogenic and Chemotactic Potential of CDMP-2","authors":"F. Y. Zhou, A. Wei, A. Diwan","doi":"10.4028/www.scientific.net/JBBTE.18.97","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.97","url":null,"abstract":"Intervertebral disc degeneration creates a significant healthcare burden on industrialized Western society. Recent research into treatment options has highlighted numerous biological strategies to replenish the native disc cells with minimal invasion, although their effectiveness and safety are still under intense investigation. This review provides an outline of the key biological therapies under research currently, including stem cells, biomaterial scaffolds and signaling molecules. Particularly, the focus will be on CDMP-2, a signaling molecule that has been found to possess properties that are therapeutic to disc degeneration. The review will assess the evidence of the molecule’s chondrogenic and chemotactic effects on disc cells and evaluate areas for further research in determining its regenerative potential in the intervertebral disc.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"53 1","pages":"118 - 97"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77011008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.1
Kai Xue, Lei Li, Q. Li
A novel design method for stiffener layout of plate and shell structures is proposed in this paper. The method is inspired by the morphogenesis mechanism of dicotyledonous venation which is featured by hierarchy and functional adaptivity. It is expected that a optimal stiffener layout can be gradually achieved if the stiffeners extend by obeying a similar growth rule as the venation. Starting from the so called “seeds”, the stiffeners grow and branch off towards the direction that optimizes the structural performance. And the stiffeners with the minimum effectiveness to the structural performance are degenerated simultaneously. During the design process, the relative density of each element is treated as the design variable. The growth and degeneration of the stiffeners are determined by the nodal and elemental sensitivity numbers respectively. The design algorithm is programmed in Python and integrated with Abaqus software which is used as the FEA preprocessor and solver. To validate the effectiveness of the proposed method, it is applied to design the stiffener layouts of some typical structures with the objective of maximizing the overall stiffness with a volume constraint.
{"title":"Venation-Inspired Growth Technique for Stiffener Layout Design of Plate and Shell Structures","authors":"Kai Xue, Lei Li, Q. Li","doi":"10.4028/www.scientific.net/JBBTE.18.1","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.1","url":null,"abstract":"A novel design method for stiffener layout of plate and shell structures is proposed in this paper. The method is inspired by the morphogenesis mechanism of dicotyledonous venation which is featured by hierarchy and functional adaptivity. It is expected that a optimal stiffener layout can be gradually achieved if the stiffeners extend by obeying a similar growth rule as the venation. Starting from the so called “seeds”, the stiffeners grow and branch off towards the direction that optimizes the structural performance. And the stiffeners with the minimum effectiveness to the structural performance are degenerated simultaneously. During the design process, the relative density of each element is treated as the design variable. The growth and degeneration of the stiffeners are determined by the nodal and elemental sensitivity numbers respectively. The design algorithm is programmed in Python and integrated with Abaqus software which is used as the FEA preprocessor and solver. To validate the effectiveness of the proposed method, it is applied to design the stiffener layouts of some typical structures with the objective of maximizing the overall stiffness with a volume constraint.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"38 1","pages":"1 - 12"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81006452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.61
M. Sader, Denisar Ismério, M. C. Andrade, G. D. Soares, I. Bastos, G. Platt
Bioceramics used in biomedical applications must exhibit specific behaviors. In scaffolds, for instance, the degradability of bioceramics is important to allow the cell ingrowth. Therefore, the dissolution of calcium phosphates increases the ionic concentrations around the interface implant–bone, favoring a more rapid bone apposition to the graft surface. The dissolution takes place under static or dynamic conditions, but the latter is usually not performed under rigorous hydrodynamic control. In the present work, two bioceramics, β-tricalcium phosphate and β-tricalcium phosphate substituted by magnesium, were produced by pressing and sintering to form disks. They were characterized by XRD, Raman, ICP, SEM, AFM and photometric test. The influence of chemical composition in the dissolution test was conducted through strict control of the hydrodynamic conditions. The disks were rotating in a precise speed, in order to produce a dissolution under the well-controlled mass transfer. Subsequently, the calcium release was evaluated in a simulated infectious environment using pH equals to circa 4. Thus, it was possible to evaluate the fraction of dissolution related to mass transfer or surface reactions for a large rotation speed range. The magnesium added to the bioceramic inhibits the total dissolution when compared to pure tricalcium phosphate, probably related to more dense and less soluble ceramic. Moreover, the mass transfer affects relatively less the magnesium tricalcium phosphate than pure tricalcium phosphate.
{"title":"Characterization and Dissolution Dynamics of Tricalcium Phosphates in Acidified Solution","authors":"M. Sader, Denisar Ismério, M. C. Andrade, G. D. Soares, I. Bastos, G. Platt","doi":"10.4028/www.scientific.net/JBBTE.18.61","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.61","url":null,"abstract":"Bioceramics used in biomedical applications must exhibit specific behaviors. In scaffolds, for instance, the degradability of bioceramics is important to allow the cell ingrowth. Therefore, the dissolution of calcium phosphates increases the ionic concentrations around the interface implant–bone, favoring a more rapid bone apposition to the graft surface. The dissolution takes place under static or dynamic conditions, but the latter is usually not performed under rigorous hydrodynamic control. In the present work, two bioceramics, β-tricalcium phosphate and β-tricalcium phosphate substituted by magnesium, were produced by pressing and sintering to form disks. They were characterized by XRD, Raman, ICP, SEM, AFM and photometric test. The influence of chemical composition in the dissolution test was conducted through strict control of the hydrodynamic conditions. The disks were rotating in a precise speed, in order to produce a dissolution under the well-controlled mass transfer. Subsequently, the calcium release was evaluated in a simulated infectious environment using pH equals to circa 4. Thus, it was possible to evaluate the fraction of dissolution related to mass transfer or surface reactions for a large rotation speed range. The magnesium added to the bioceramic inhibits the total dissolution when compared to pure tricalcium phosphate, probably related to more dense and less soluble ceramic. Moreover, the mass transfer affects relatively less the magnesium tricalcium phosphate than pure tricalcium phosphate.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"30 1","pages":"61 - 71"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91017339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.73
A. Sterodimas, V. Kalodimou, B. Nicaretta
Background Numerous studies have provided data on the efficacy of ADSCs, supporting their use in current and future clinical applications. This is the first study to our knowledge, which aims at comparing the cell viability and the absolute number of mesenchymal stem cells and ADSCs from three different approaches of preparing adipose tissue for autologous transplantation. Patients & MethodsAdipose tissue was taken from the hip/thigh region of 8 female donors undergoing liposuction. From every patient, there was sent three different fat samples: lipoaspirated fat decanted (A), lipoaspirated fat prepared by normal saline washing (B) and stromal enriched lipograft (C). Multi-parameter flow cytometry to determine the absolute number and viability of ADSCs was performed. ResultsThe mean absolute cell counts per gram of adipose tissue were 8.33x10⁶ in samples A and 5.97x10⁶ in sample C. In B samples the mean absolute cell counts per gram of adipose tissue were 2.13x10⁶. The presence of ADSCs specific markers in all the C samples showed high expression (> 95%) in the positive markers and low expression (< 2%) in the negative markers and are essential to validate the purity of adipose stem cells in a sample. ConclusionThe results obtained from the analysis of eight different donors of lipoaspirate indicate that the highest absolute number of viable adipose derived stem cells is found in the Stromal Enriched Lipograft (sample C). Their purity was confirmed by the high expression (> 95%) in the positive markers and low expression (< 2%) in the negative markers.
{"title":"Adipose Derived Stem Cells Characterization from Human Lipoaspirate: A Comparative Study","authors":"A. Sterodimas, V. Kalodimou, B. Nicaretta","doi":"10.4028/www.scientific.net/JBBTE.18.73","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.73","url":null,"abstract":"Background Numerous studies have provided data on the efficacy of ADSCs, supporting their use in current and future clinical applications. This is the first study to our knowledge, which aims at comparing the cell viability and the absolute number of mesenchymal stem cells and ADSCs from three different approaches of preparing adipose tissue for autologous transplantation. Patients & MethodsAdipose tissue was taken from the hip/thigh region of 8 female donors undergoing liposuction. From every patient, there was sent three different fat samples: lipoaspirated fat decanted (A), lipoaspirated fat prepared by normal saline washing (B) and stromal enriched lipograft (C). Multi-parameter flow cytometry to determine the absolute number and viability of ADSCs was performed. ResultsThe mean absolute cell counts per gram of adipose tissue were 8.33x10⁶ in samples A and 5.97x10⁶ in sample C. In B samples the mean absolute cell counts per gram of adipose tissue were 2.13x10⁶. The presence of ADSCs specific markers in all the C samples showed high expression (> 95%) in the positive markers and low expression (< 2%) in the negative markers and are essential to validate the purity of adipose stem cells in a sample. ConclusionThe results obtained from the analysis of eight different donors of lipoaspirate indicate that the highest absolute number of viable adipose derived stem cells is found in the Stromal Enriched Lipograft (sample C). Their purity was confirmed by the high expression (> 95%) in the positive markers and low expression (< 2%) in the negative markers.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"3 1","pages":"73 - 83"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73661720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-01DOI: 10.4028/www.scientific.net/JBBTE.18.29
Jie Zhu, Yueming Zhang, Zhilong Tao, N. Xu, Li Qun Wang, X. Zhu
We reported the preparation of surface modified poly(butylene terephthalate)-co-poly(butylene succinate)-b-poly(ethylene glycol) (i.e. PBT-co-PBS/PEG) films by three methods: silk fibroin coating, SO2 plasma treatment and silk fibroin anchoring. The obtained composite films were named SF/(PBT-co-PBS/PEG), SO2/(PBT-co-PBS/PEG) and SF/SO2/(PBT-co-PBS/PEG), respectively. Their surface properties were characterized by contact angles, surface energies and XPS. The biocompatibility of the films were further evaluated by the morphology, attachment, proliferation and viability of human salivary epithelial cells (HSG cells). Results revealed that SF/SO2/(PBT-co-PBS/PEG) possessed the high surface free energy (59.67 mJ/m2) and could immobilize a great amount of fibroin (SF surface coverage: 26.39 wt%), which attributed to the formation of such polar groups as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. The cell tests suggested that the silk fibroin anchoring could significantly enhance the biocompatibility of PBT-co-PBS/PEG, which implied the potential application of fibroin modified PBT-co-PBS/PEG for clinical HSG cells transplantation in artificial salivary gland constructs.
{"title":"The Preparation of Silk Fibroin Modified PBT-co-PBS/PEG Composite Films and their Effects on Clinical Human Salivary Epithelial Cells Transplantation","authors":"Jie Zhu, Yueming Zhang, Zhilong Tao, N. Xu, Li Qun Wang, X. Zhu","doi":"10.4028/www.scientific.net/JBBTE.18.29","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.18.29","url":null,"abstract":"We reported the preparation of surface modified poly(butylene terephthalate)-co-poly(butylene succinate)-b-poly(ethylene glycol) (i.e. PBT-co-PBS/PEG) films by three methods: silk fibroin coating, SO2 plasma treatment and silk fibroin anchoring. The obtained composite films were named SF/(PBT-co-PBS/PEG), SO2/(PBT-co-PBS/PEG) and SF/SO2/(PBT-co-PBS/PEG), respectively. Their surface properties were characterized by contact angles, surface energies and XPS. The biocompatibility of the films were further evaluated by the morphology, attachment, proliferation and viability of human salivary epithelial cells (HSG cells). Results revealed that SF/SO2/(PBT-co-PBS/PEG) possessed the high surface free energy (59.67 mJ/m2) and could immobilize a great amount of fibroin (SF surface coverage: 26.39 wt%), which attributed to the formation of such polar groups as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. The cell tests suggested that the silk fibroin anchoring could significantly enhance the biocompatibility of PBT-co-PBS/PEG, which implied the potential application of fibroin modified PBT-co-PBS/PEG for clinical HSG cells transplantation in artificial salivary gland constructs.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"10 1","pages":"29 - 42"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88436733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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