Pub Date : 2013-11-18DOI: 10.4172/1662-100X.1000117
Zou Feng, Zhang Shangkun, Li Yan, Chen Ying, Li Lijiang
We investigated the expression profile of microRNA (miRNA) in ER-α36-mediated transplantable tumours. SGC7901 cell lines of higher or lower ER-α36 expression were constructed with a lentivirus vector. Five million SGC7901 cells were injected into nude mice to form a gastric xenografted carcinoma in the subcutaneous tissue of nude mice. Observation was performed for 30 days. The weight and propagation of the tumours were determined. miRNA expression profiles of the three SGC7901 cell lines were analysed by miRNA microarray. miR-143 expression in transplantable tumours was determined by real-time PCR using TaqMan assay. The expression level of ER-α36 was proportional to the size and propagation of gastric xenografted carcinomas in nude mice. The expression level of miR-143 significantly decreased in highly expressing ER-α36 SGC7901 cells and significantly increased in lowly expressing ER-α36 SGC7901 cells. miR-143 expression results in transplantable tumours amongst nude mice were consistent with the miRNA microarray findings. The expression of miR-143 was suppressed by high ER-α36 expression. These findings suggest that deregulation of miR-143 plays an important role in the progression of ER-α36-mediated gastric xenografted carcinoma.
{"title":"miR-143 is Associated with ER-α36 Expression Gastric Carcinoma of Xenografted Model","authors":"Zou Feng, Zhang Shangkun, Li Yan, Chen Ying, Li Lijiang","doi":"10.4172/1662-100X.1000117","DOIUrl":"https://doi.org/10.4172/1662-100X.1000117","url":null,"abstract":"We investigated the expression profile of microRNA (miRNA) in ER-α36-mediated transplantable tumours. SGC7901 cell lines of higher or lower ER-α36 expression were constructed with a lentivirus vector. Five million SGC7901 cells were injected into nude mice to form a gastric xenografted carcinoma in the subcutaneous tissue of nude mice. Observation was performed for 30 days. The weight and propagation of the tumours were determined. miRNA expression profiles of the three SGC7901 cell lines were analysed by miRNA microarray. miR-143 expression in transplantable tumours was determined by real-time PCR using TaqMan assay. The expression level of ER-α36 was proportional to the size and propagation of gastric xenografted carcinomas in nude mice. The expression level of miR-143 significantly decreased in highly expressing ER-α36 SGC7901 cells and significantly increased in lowly expressing ER-α36 SGC7901 cells. miR-143 expression results in transplantable tumours amongst nude mice were consistent with the miRNA microarray findings. The expression of miR-143 was suppressed by high ER-α36 expression. These findings suggest that deregulation of miR-143 plays an important role in the progression of ER-α36-mediated gastric xenografted carcinoma.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79584940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-11-04DOI: 10.4172/1662-100X.1000118
Q. Meiyu, Yuchang Yao, Hong Ma, W. Jiabo, Zhao Xiaochuan, Liu Li, T. Xiaodong, Lili Zhang, S. Zhang, Sun Fang
OPU together with IVF has been taken as another challenge to the animal reproduction. While under so many years development, OPU has been considered as a mature technology. No many breakthroughs might happen to this technology. We concluded the history, present of bovine OPU, its advantages and disadvantages, factors that may influence its efficiency in this article. We hope to offer a reference for the development of bovine OPU technology in the future.
{"title":"Transvaginal Ultrasound-guided Ovum Pick-up(OPU) in Cattle","authors":"Q. Meiyu, Yuchang Yao, Hong Ma, W. Jiabo, Zhao Xiaochuan, Liu Li, T. Xiaodong, Lili Zhang, S. Zhang, Sun Fang","doi":"10.4172/1662-100X.1000118","DOIUrl":"https://doi.org/10.4172/1662-100X.1000118","url":null,"abstract":"OPU together with IVF has been taken as another challenge to the animal reproduction. While under so many years development, OPU has been considered as a mature technology. No many breakthroughs might happen to this technology. We concluded the history, present of bovine OPU, its advantages and disadvantages, factors that may influence its efficiency in this article. We hope to offer a reference for the development of bovine OPU technology in the future.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91180614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-17DOI: 10.4172/1662-100X.1000113
Yueqi Zhu, Hongwei Zhang, Xuehua Zhang
In this paper, we studied the application of Nano-TiO2 Modified membrane technology in municipal wastewater treatment, which has not been previously reported to exhibit in this area. Ozone (O3) aeration can produce a large number of high oxidizing free radicals (hydroxyl radical HO•) to degrade organic matters when ozone contacts with nano- TiO2 Modified membrane, which process is called catalytic ozone oxidation process. This approach is a new frontier of municipal wastewater treatment, which has excellent reactive activity and degradation of organic compounds without the need of catalyst recycling. Our purpose is to remove organic matters by ozone aeration pre-treatment and nano-TiO2 modified membrane. This method is more efficient since it combines both chemical oxidation and physical filtration. In this study, municipal sewage is called raw water. Polyvinylidene fluoride (PVDF) and the nano-TiO2 modified PVDF ultrafiltration membranes are selected as experimental materials. Results suggest that the removal rate of organic matters with this new method (TiO2+O3) is 66.4%, which is 13.3% higher than original membrane with O3 (PVDF+O3) under the same condition. Therefore we can extract that, this part of contribution belongs to catalytic ozone oxidation process on TiO2 modified membrane. Compared with traditional PVDF membrane filtration, whose removal rate is 38.7%, this novel approach has a removal rate 27.7% higher.
{"title":"Study on Catalytic Ozone Oxidation with Nano-TiO2 Modified Membrane for Treatment of Municipal Wastewater","authors":"Yueqi Zhu, Hongwei Zhang, Xuehua Zhang","doi":"10.4172/1662-100X.1000113","DOIUrl":"https://doi.org/10.4172/1662-100X.1000113","url":null,"abstract":"In this paper, we studied the application of Nano-TiO2 Modified membrane technology in municipal wastewater treatment, which has not been previously reported to exhibit in this area. Ozone (O3) aeration can produce a large number of high oxidizing free radicals (hydroxyl radical HO•) to degrade organic matters when ozone contacts with nano- TiO2 Modified membrane, which process is called catalytic ozone oxidation process. This approach is a new frontier of municipal wastewater treatment, which has excellent reactive activity and degradation of organic compounds without the need of catalyst recycling. Our purpose is to remove organic matters by ozone aeration pre-treatment and nano-TiO2 modified membrane. This method is more efficient since it combines both chemical oxidation and physical filtration. In this study, municipal sewage is called raw water. Polyvinylidene fluoride (PVDF) and the nano-TiO2 modified PVDF ultrafiltration membranes are selected as experimental materials. Results suggest that the removal rate of organic matters with this new method (TiO2+O3) is 66.4%, which is 13.3% higher than original membrane with O3 (PVDF+O3) under the same condition. Therefore we can extract that, this part of contribution belongs to catalytic ozone oxidation process on TiO2 modified membrane. Compared with traditional PVDF membrane filtration, whose removal rate is 38.7%, this novel approach has a removal rate 27.7% higher.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89735808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-15DOI: 10.4172/1662-100X.1000114
Guibao Cao, Shuangling Wang, B. Wei, Yilong Yin, Gongping Yang
To get new insights into the function and structure of the brain,neuroanatomists need to build 3D reconstructions of brain tissue from electron microscopy (EM) images. One key step towards this is to get automatic segmentation of neuronal structures depicted in stacks of electron microscopy images. However, due to the visual complex appearance of neuronal structures, it is challenging to automatically segment membranes in the EM images. Based on Convolutional Neural Network (CNN) and Random Forest classifier (RF), a hybrid CNN-RF method for EM neuron segmentation is presented. CNN as a feature extractor is trained firstly, and then well behaved features are learned with the trained feature extractor automatically. Finally, Random Forest classifier is trained on the learned features to perform neuron segmentation. Experiments have been conducted on the benchmarks for the ISBI2012 EM Segmentation Challenge, and the proposed method achieves the effectiveness results: The Rand error, Warping error and Pixel error attains to 0.109388991, 0.001455688 and 0.072129307, respectively.
{"title":"A Hybrid Cnn-Rf Method for Electron Microscopy Images Segmentation","authors":"Guibao Cao, Shuangling Wang, B. Wei, Yilong Yin, Gongping Yang","doi":"10.4172/1662-100X.1000114","DOIUrl":"https://doi.org/10.4172/1662-100X.1000114","url":null,"abstract":"To get new insights into the function and structure of the brain,neuroanatomists need to build 3D reconstructions of brain tissue from electron microscopy (EM) images. One key step towards this is to get automatic segmentation of neuronal structures depicted in stacks of electron microscopy images. However, due to the visual complex appearance of neuronal structures, it is challenging to automatically segment membranes in the EM images. Based on Convolutional Neural Network (CNN) and Random Forest classifier (RF), a hybrid CNN-RF method for EM neuron segmentation is presented. CNN as a feature extractor is trained firstly, and then well behaved features are learned with the trained feature extractor automatically. Finally, Random Forest classifier is trained on the learned features to perform neuron segmentation. Experiments have been conducted on the benchmarks for the ISBI2012 EM Segmentation Challenge, and the proposed method achieves the effectiveness results: The Rand error, Warping error and Pixel error attains to 0.109388991, 0.001455688 and 0.072129307, respectively.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89951562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-09-20DOI: 10.4172/1662-100X.1000112
Y. Song, W. Bao, B. Li, G. Yang, Pang
To study the antioxidant effect of the aqueous extracts of Typhonium giganteum Engl. tuber (AEoTGE) and ethanol extracts of Typhonium giganteum Engl. tuber (EEoTGE) in vitro and in vivo, which may be the possible mechanism of its bioactivity efficacy. The antioxidant activity in vitro was evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging rate with Vitamin C as a positive control. The antioxidant activity in vivo was carried out to investigate the effects of AEoTGE and EEoTGE on D-galactose caused aging model mice by determining liver tissues’ superoxide dismutase (SOD) activities. The results indicated that AEoTGE and EEoTGE have good antioxidant activity in vivo and vitro. The antioxidant effect of AEoTGE is better than that of EEoTGE.
{"title":"Study on Antioxidant Activity of Typhonium giganteum Engl. Tuber Extracts in vitro and in vivo","authors":"Y. Song, W. Bao, B. Li, G. Yang, Pang","doi":"10.4172/1662-100X.1000112","DOIUrl":"https://doi.org/10.4172/1662-100X.1000112","url":null,"abstract":"To study the antioxidant effect of the aqueous extracts of Typhonium giganteum Engl. tuber (AEoTGE) and ethanol extracts of Typhonium giganteum Engl. tuber (EEoTGE) in vitro and in vivo, which may be the possible mechanism of its bioactivity efficacy. The antioxidant activity in vitro was evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging rate with Vitamin C as a positive control. The antioxidant activity in vivo was carried out to investigate the effects of AEoTGE and EEoTGE on D-galactose caused aging model mice by determining liver tissues’ superoxide dismutase (SOD) activities. The results indicated that AEoTGE and EEoTGE have good antioxidant activity in vivo and vitro. The antioxidant effect of AEoTGE is better than that of EEoTGE.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86117621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-09-10DOI: 10.4172/1662-100X.1000110
Yuangang Liu, Yan Bai, Shi-Bin Wang, A. Chen, Wen-Guo Wu
To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.
{"title":"Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate(APornA) Microcapsules","authors":"Yuangang Liu, Yan Bai, Shi-Bin Wang, A. Chen, Wen-Guo Wu","doi":"10.4172/1662-100X.1000110","DOIUrl":"https://doi.org/10.4172/1662-100X.1000110","url":null,"abstract":"To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"1 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2013-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83045540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-09-10DOI: 10.4172/1662-100X.1000111
A. Ramazani, N. Arsalani, H. K. Khiabani, Amir Mohammad Goganian
In this work, we aim to study the development and characterization of thermosensitive polymer-coated iron oxide nanoparticles as a novel ferroFluid (fF) with thermosensitive properties. For this purpose, polymerization was conducted in the presence of various ratios of N-isopropylacrylamide (NIPAAm), acrylamide (AAm) and N-vinylpyrrolidone (NVP) as monomers, and N,N´-azobisisobutyronitrile (AIBN) as an initiator. Particles having average sizes of 8 nm and 8–10 nm were respectively observed for Fe3O4/fF and Fe3O4/polymers. As visualized by transmission electron microscopy (TEM) images, both the coated and uncoated iron oxide nanoparticles were uniform in shape and seem to have been monodispersed. Vibrating sample magnetometry (VSM) measurements of Fe3O4/VTES-fF and Fe3O4/Poly (NIPAAm- AAm NVP) at room temperature showed that they had a superparamagnetic nature with saturation magnetization values of 23.14 emu/g and 4.33 emu/g, respectively. In the thermosensitivity analysis, the lower critical solution temperature (LCST) was around 36-40°C, as determined by UV-Vis absorption spectroscopy. Furthermore, the polymerization of (NIPAAm-AAm-NVP) with the surface modified magnetic ferroFluid was confirmed by Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). These aqueous, stable, magnetic nanoparticles coated with temperature-sensitive polymers have attracted great attention because of their various applications in the fields of biotechnology and medicine.
{"title":"Development and Characterization of Thermosensitive Polymer- CoatedIron Oxide Nanoparticles as a Novel Ferrofluid","authors":"A. Ramazani, N. Arsalani, H. K. Khiabani, Amir Mohammad Goganian","doi":"10.4172/1662-100X.1000111","DOIUrl":"https://doi.org/10.4172/1662-100X.1000111","url":null,"abstract":"In this work, we aim to study the development and characterization of thermosensitive polymer-coated iron oxide nanoparticles as a novel ferroFluid (fF) with thermosensitive properties. For this purpose, polymerization was conducted in the presence of various ratios of N-isopropylacrylamide (NIPAAm), acrylamide (AAm) and N-vinylpyrrolidone (NVP) as monomers, and N,N´-azobisisobutyronitrile (AIBN) as an initiator. Particles having average sizes of 8 nm and 8–10 nm were respectively observed for Fe3O4/fF and Fe3O4/polymers. As visualized by transmission electron microscopy (TEM) images, both the coated and uncoated iron oxide nanoparticles were uniform in shape and seem to have been monodispersed. Vibrating sample magnetometry (VSM) measurements of Fe3O4/VTES-fF and Fe3O4/Poly (NIPAAm- AAm NVP) at room temperature showed that they had a superparamagnetic nature with saturation magnetization values of 23.14 emu/g and 4.33 emu/g, respectively. In the thermosensitivity analysis, the lower critical solution temperature (LCST) was around 36-40°C, as determined by UV-Vis absorption spectroscopy. Furthermore, the polymerization of (NIPAAm-AAm-NVP) with the surface modified magnetic ferroFluid was confirmed by Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). These aqueous, stable, magnetic nanoparticles coated with temperature-sensitive polymers have attracted great attention because of their various applications in the fields of biotechnology and medicine.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"18 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2013-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84237728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-06-01DOI: 10.4028/www.scientific.net/JBBTE.17.59
Bahare Asgari, M. Azami, Afsaneh Amiri, A. I. Imani Fooladi, M. Nourani
Apatite phase Calcium and Magnesium Phosphate doped nanocomposite scaffold has been synthesized in physiological environment by gelatin hydrogel double diffusion technique. Several analytical methods, such as X-ray diffraction (XRD), infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDS) and scanning electron microscopy (SEM) were applied to characterize physicochemical properties of the studied samples.The results showed that nanocomposite scaffolds were porous with three-dimensionally interconnected microstructure, pore size ranging from 200 to 300 μm nanocrystalline precipitated minerals were dispersed evenly among gelatin fibers. A mineral containing amorphous calcium phosphate and brushite precipitate was formed within the gelatin matrix at 4°C. After incubation in SBF solution at 37°C for 7 days, the mineral phase was changed to nanocrystalline hydroxyapatite. It should be well-known that precursor phases inside a scaffold implanted into the bone are equal to biomimetic adaptation of precursors to hydroxyapatite that is very similar to the bone and has an attentive level of biocompatibility. Therefore, the result confirms the significance of biomimetic calcium and magnesium phosphate bone tissue scaffolds in developing new biomaterials for bone regeneration.
{"title":"Bone Scaffold Biomimetics Based on Gelatin Hydrogel Mineralization","authors":"Bahare Asgari, M. Azami, Afsaneh Amiri, A. I. Imani Fooladi, M. Nourani","doi":"10.4028/www.scientific.net/JBBTE.17.59","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.17.59","url":null,"abstract":"Apatite phase Calcium and Magnesium Phosphate doped nanocomposite scaffold has been synthesized in physiological environment by gelatin hydrogel double diffusion technique. Several analytical methods, such as X-ray diffraction (XRD), infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDS) and scanning electron microscopy (SEM) were applied to characterize physicochemical properties of the studied samples.The results showed that nanocomposite scaffolds were porous with three-dimensionally interconnected microstructure, pore size ranging from 200 to 300 μm nanocrystalline precipitated minerals were dispersed evenly among gelatin fibers. A mineral containing amorphous calcium phosphate and brushite precipitate was formed within the gelatin matrix at 4°C. After incubation in SBF solution at 37°C for 7 days, the mineral phase was changed to nanocrystalline hydroxyapatite. It should be well-known that precursor phases inside a scaffold implanted into the bone are equal to biomimetic adaptation of precursors to hydroxyapatite that is very similar to the bone and has an attentive level of biocompatibility. Therefore, the result confirms the significance of biomimetic calcium and magnesium phosphate bone tissue scaffolds in developing new biomaterials for bone regeneration.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"1 1","pages":"59 - 69"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86136736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-06-01DOI: 10.4028/www.scientific.net/JBBTE.17.45
Q. Bao
Y, Zn and Ca were selected to develop a Magnesium alloy, Mg-Y-Ca-Zn for biomedical application due to the good biocompatibility of Zn and Ca elements. Microstructure, mechanical properties and corrosion properties of the Mg-Y-Ca-Zn alloy have been investigated using both optical and scanning electron microscope. In the as-cast condition, primary α-Mg matrix and second phase are mainly distributed along grain boundary. After solution treatment, the distribution of second phase decreased and after aging, there are many second phases precipitated along the grain boundary and inside the grains. The hardness of as-cast samples was low and increased after solution treatment and aging. An aged sample had more corrosion resistance than as-cast and solution treatment alloys.
{"title":"Microstructure, Mechanical and Corrosion Properties of Mg-Y-Ca-Zn Alloy for Biomedical Applications","authors":"Q. Bao","doi":"10.4028/www.scientific.net/JBBTE.17.45","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.17.45","url":null,"abstract":"Y, Zn and Ca were selected to develop a Magnesium alloy, Mg-Y-Ca-Zn for biomedical application due to the good biocompatibility of Zn and Ca elements. Microstructure, mechanical properties and corrosion properties of the Mg-Y-Ca-Zn alloy have been investigated using both optical and scanning electron microscope. In the as-cast condition, primary α-Mg matrix and second phase are mainly distributed along grain boundary. After solution treatment, the distribution of second phase decreased and after aging, there are many second phases precipitated along the grain boundary and inside the grains. The hardness of as-cast samples was low and increased after solution treatment and aging. An aged sample had more corrosion resistance than as-cast and solution treatment alloys.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"23 1","pages":"45 - 51"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83232123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-06-01DOI: 10.4028/www.scientific.net/JBBTE.17.1
X. Tian, M. Li, Xiongbaio Chen
Tissue scaffolds play a vital role in tissue engineering by providing a native tissue-mimicking environment for cell proliferation and differentiation as well as tissue regeneration. Fabrication of tissue scaffolds has been drawing increasing research attention and a number of fabrication techniques have been developed. To better mimic the microenvironment of native tissues, novel techniques have emerged in recent years to encapsulate cells into the engineered scaffolds during the scaffold fabrication process. Among them, bio-Rapid-Prototyping (bioRP) techniques, by which scaffolds with encapsulated cells can be fabricated with controlled internal microstructure and external shape, shows significant promise. It is noted in the bioRP processes, cells may be continuously subjected to environmental stresses such as mechanical, electrical forces and laser exposure. If the stress is greater than a certain level, the cell membrane may be ruptured, leading to the so-called process-induced cell damage. This paper reviews various cell encapsulation techniques for tissue scaffold fabrication, with emphasis on the bioRP technologies and their technical features. To understand the process-induced cell damage in the bioRP processes, this paper also surveys the cell damage mechanisms under different stresses. The process-induced cell damage models are also examined to provide a cue to the cell viability preservation in the fabrication process. Discussions on further improvements of bioRP technologies are given and ongoing research into mechanical cell damage mechanism are also suggested in this review.
{"title":"Bio-Rapid-Prototyping of Tissue Engineering Scaffolds and the Process-Induced Cell Damage","authors":"X. Tian, M. Li, Xiongbaio Chen","doi":"10.4028/www.scientific.net/JBBTE.17.1","DOIUrl":"https://doi.org/10.4028/www.scientific.net/JBBTE.17.1","url":null,"abstract":"Tissue scaffolds play a vital role in tissue engineering by providing a native tissue-mimicking environment for cell proliferation and differentiation as well as tissue regeneration. Fabrication of tissue scaffolds has been drawing increasing research attention and a number of fabrication techniques have been developed. To better mimic the microenvironment of native tissues, novel techniques have emerged in recent years to encapsulate cells into the engineered scaffolds during the scaffold fabrication process. Among them, bio-Rapid-Prototyping (bioRP) techniques, by which scaffolds with encapsulated cells can be fabricated with controlled internal microstructure and external shape, shows significant promise. It is noted in the bioRP processes, cells may be continuously subjected to environmental stresses such as mechanical, electrical forces and laser exposure. If the stress is greater than a certain level, the cell membrane may be ruptured, leading to the so-called process-induced cell damage. This paper reviews various cell encapsulation techniques for tissue scaffold fabrication, with emphasis on the bioRP technologies and their technical features. To understand the process-induced cell damage in the bioRP processes, this paper also surveys the cell damage mechanisms under different stresses. The process-induced cell damage models are also examined to provide a cue to the cell viability preservation in the fabrication process. Discussions on further improvements of bioRP technologies are given and ongoing research into mechanical cell damage mechanism are also suggested in this review.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"3 1","pages":"1 - 23"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79707250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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