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Journal of biomedical materials research. Part B, Applied biomaterials最新文献

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Improving osseointegration and antimicrobial properties of titanium implants with black phosphorus nanosheets-hydroxyapatite composite coatings for vascularized bone regeneration 用黑磷纳米片-羟基磷灰石复合涂层改善钛植入物的骨结合和抗菌性能,促进血管化骨再生。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-23 DOI: 10.1002/jbm.b.35403
Shilong Ma, Ruize Sun, Yuhui Wang, Yan Wei, Haofeng Xu, Xuanyu Liu, Ziwei Liang, Liqin Zhao, Yinchun Hu, Xiaojie Lian, Meiqing Guo, Di Huang

For decades, titanium implants have shown impressive advantages in bone repair. However, the preparation of implants with excellent antimicrobial properties as well as better osseointegration ability remains difficult for clinical application. In this study, black phosphorus nanosheets (BPNSs) were doped into hydroxyapatite (HA) coatings using electrophoretic deposition. The coatings' surface morphology, roughness, water contact angle, photothermal properties, and antibacterial properties were investigated. The BP/HA coating exhibited a surface roughness of 59.1 nm, providing an ideal substrate for cell attachment and growth. The water contact angle on the BP/HA coating was measured to be approximately 8.55°, indicating its hydrophilic nature. The BPNSs demonstrated efficient photothermal conversion, with a temperature increase of 42.2°C under laser irradiation. The BP/HA composite coating exhibited a significant reduction in bacterial growth, with inhibition rates of 95.6% and 96.1% against Staphylococcus aureus and Escherichia coli. In addition, the cytocompatibility of the composite coating was evaluated by cell adhesion, CCK8 and AM/PI staining; the effect of the composite coating in promoting angiogenesis was assessed by scratch assay, transwell assay, and protein blotting; and the osteoinductivity of the composite coating was evaluated by alkaline phosphatase assay, alizarin red staining, and Western blot. The results showed that the BP/HA composite coating exhibited superior performance in promoting biological functions such as cell proliferation and adhesion, antibacterial activity, osteogenic differentiation, and angiogenesis, and had potential applications in vascularized bone regeneration.

几十年来,钛植入物在骨修复方面已显示出令人印象深刻的优势。然而,要制备出抗菌性能优异、骨结合能力更强的植入体,在临床应用中仍然存在困难。本研究采用电泳沉积法将黑磷纳米片(BPNSs)掺杂到羟基磷灰石(HA)涂层中。研究了涂层的表面形态、粗糙度、水接触角、光热特性和抗菌特性。BP/HA 涂层的表面粗糙度为 59.1 nm,为细胞的附着和生长提供了理想的基底。经测量,BP/HA 涂层的水接触角约为 8.55°,表明其具有亲水性。BPNSs 表现出高效的光热转换能力,在激光照射下温度升高了 42.2°C。BP/HA 复合涂层能显著减少细菌生长,对金黄色葡萄球菌和大肠杆菌的抑制率分别为 95.6% 和 96.1%。此外,还通过细胞粘附、CCK8 和 AM/PI 染色评估了复合涂层的细胞相容性;通过划痕试验、transwell 试验和蛋白印迹评估了复合涂层促进血管生成的效果;通过碱性磷酸酶试验、茜素红染色和 Western 印迹评估了复合涂层的骨诱导性。结果表明,BP/HA 复合涂层在促进细胞增殖和粘附、抗菌、成骨分化和血管生成等生物功能方面表现优异,在血管骨再生方面具有潜在的应用价值。
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引用次数: 0
Association between blood markers and the progression of osseointegration in percutaneous prostheses patients—A pilot study 经皮假体患者血液标记物与骨结合进展之间的关系--一项试验性研究。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-08 DOI: 10.1002/jbm.b.35398
Andrew Miller, Sujee Jeyapalina, Jayant P. Agarwal, James Peter Beck

Patients implanted with osseointegrated (OI) prosthetic systems have reported vastly improved upper and lower extremity prosthetic function compared with their previous experience with socket-suspension systems. However, OI systems have been associated with superficial and deep-bone infections and implant loosening due, in part, to a failure of the osseointegration process. Although monitoring the osseointegration using circulating biomarkers has clinical relevance for understanding the progression of osseointegration with these devices, it has yet to be established. Ten patients were enrolled in this study. Blood samples were collected at pre-selected times, starting before implantation surgery, and continuing to 12 months after the second surgery. Bone formation markers, bone resorption markers, and circulating amino acids were measured from blood samples. A linear mixed model was generated for each marker, incorporating patient ID and age with the normalized marker value as the response variable. Post hoc comparisons were made between 1 week before Stage 1 Surgery and all subsequent time points for each marker, followed by multiple testing corrections. Serial radiographic imaging of the residual limb containing the implant was obtained during follow-up, and the cortical index (CI) was calculated for the bone at the porous region of the device. Two markers of bone formation, specifically bone-specific alkaline phosphatase (Bone-ALP) and amino-terminal propeptide of type I procollagen (PINP), exhibited significant increases when compared with the baseline levels of unloaded residual bone prior to the initial surgery, and they subsequently returned to their baseline levels by the 12-month mark. Patients who experienced clinically robust osseointegration experienced increased cortical bone thickness at the porous coated region of the device. A medium correlation was observed between Bone-ALP and the porous CI values up to PoS2-M1 (p = .056), while no correlation was observed for PINP. An increase in bone formation markers and the lack of change observed in bone resorption markers likely reflect increased cortical bone formation induced by the end-loading design of the Utah OI device used in this study. A more extensive study is required to validate the correlation observed between Bone-ALP and porous CI values.

据报道,植入骨结合(OI)假肢系统的患者的上肢和下肢假肢功能比以前使用插座悬吊系统时有了很大改善。然而,骨结合义肢系统也与浅层和深层骨感染以及假体松动有关,部分原因是骨结合过程失败。虽然使用循环生物标志物监测骨结合情况对了解这些设备的骨结合进展具有临床意义,但这一意义尚未得到确立。本研究共招募了十名患者。从植入手术前开始,到第二次手术后的 12 个月,在预先选定的时间采集血液样本。从血液样本中测量骨形成标志物、骨吸收标志物和循环氨基酸。针对每种标记物生成一个线性混合模型,将患者 ID 和年龄与归一化标记物值作为响应变量。在第一阶段手术前一周与随后所有时间点之间对每个标记物进行事后比较,然后进行多重检验校正。在随访期间,对植入假体的残肢进行了连续的放射成像检查,并计算了假体多孔区域骨质的皮质指数(CI)。与初次手术前未加载残余骨的基线水平相比,骨形成的两个标志物,特别是骨特异性碱性磷酸酶(Bone-ALP)和 I 型胶原蛋白的氨基末端前肽(PINP),都有显著增加,随后在 12 个月时恢复到基线水平。在临床上经历了稳固骨结合的患者,其装置多孔涂层区域的皮质骨厚度有所增加。在 Bone-ALP 和多孔 CI 值之间观察到中等相关性(PoS2-M1)(p = .056),而 PINP 则没有观察到相关性。骨形成标志物的增加和骨吸收标志物的无变化可能反映了本研究中使用的犹他州 OI 装置的末端加载设计诱导了皮质骨形成的增加。需要进行更广泛的研究,以验证在 Bone-ALP 和多孔 CI 值之间观察到的相关性。
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引用次数: 0
Injectable macroporous naturally-derived apatite bone cement as a potential trabecular bone substitute 可注射的大孔天然磷灰石骨水泥作为一种潜在的骨小梁替代物。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-08 DOI: 10.1002/jbm.b.35397
Vimal Kumar Dewangan, T. S. Sampath Kumar, Mukesh Doble, Viju Daniel Varghese

In this study, we have formulated a novel apatite bone cements derived from natural sources (i.e. eggshell and fishbone) with improved qualities that is, porosity, resorbability, biological activity, and so forth. The naturally-derived apatite bone cement (i.e. FBDEAp) was prepared by mixing hydroxyapatite (synthesized from fishbone) and tricalcium phosphate (synthesized from eggshell) as a solid phase with a liquid phase (a dilute acidic blend of cement binding accelerator and biopolymers like gelatin and chitosan) with polysorbate (as liquid porogen) to get a desired bone cement paste. The prepared cement paste sets within the clinically acceptable setting time (≤20 min), easily injectable (>85%) through hands and exhibits physiological pH stability (7.3–7.4). The pure apatite phased bone cement was confirmed by x-ray diffraction and Fourier transform infrared spectroscopy analyses. The FBDEAp bone cement possesses acceptable compressive strength (i.e. 5–7 MPa) within trabecular bone range and is resorbable up to 28% in simulated body fluid solution within 12 weeks of incubation at physiological conditions. The FBDEAp is macroporous in nature (average pore size ~50–400 μm) with interconnected pores verified by SEM and micro-CT analyses. The FBDEAp showed significantly increased MG63 cell viability (>125% after 72 h), cell adhesion, proliferation, and key osteogenic genes expression levels (up to 5–13 folds) compared to the synthetically derived, synthetic and eggshell derived as well as synthetic and fishbone derived bone cements. Thus, we strongly believe that our prepared FBDEAp bone cement can be used as potential trabecular bone substitute in orthopedics.

在这项研究中,我们配制了一种新型的天然来源(即蛋壳和鱼骨)磷灰石骨水泥,其孔隙率、可吸收性、生物活性等质量均有所提高。天然磷灰石骨水泥(即 FBDEAp)的制备方法是将羟基磷灰石(从鱼骨中合成)和磷酸三钙(从蛋壳中合成)作为固相与液相(骨水泥结合促进剂以及明胶和壳聚糖等生物聚合物的稀酸性混合物)和聚山梨醇酯(作为液体致孔剂)混合,得到所需的骨水泥浆。制备的骨水泥浆在临床可接受的凝固时间(≤20 分钟)内凝固,易于用手注射(>85%),并具有生理 pH 值稳定性(7.3-7.4)。X 射线衍射和傅立叶变换红外光谱分析证实了这种纯磷灰石相骨水泥。FBDEAp 骨水泥在骨小梁范围内具有可接受的抗压强度(即 5-7 兆帕),在生理条件下培养 12 周后,在模拟体液溶液中的吸收率可达 28%。经扫描电子显微镜(SEM)和显微计算机断层扫描(Micro-CT)分析验证,FBDEAp 具有大孔性质(平均孔径约为 50-400 μm),孔与孔之间相互连接。与合成衍生骨水泥、合成和蛋壳衍生骨水泥以及合成和鱼骨衍生骨水泥相比,FBDEAp 在 MG63 细胞存活率(72 小时后大于 125%)、细胞粘附性、增殖性和关键成骨基因表达水平(高达 5-13 倍)方面均有显著提高。因此,我们坚信我们制备的 FBDEAp 骨水泥可作为骨科中潜在的骨小梁替代物。
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引用次数: 0
Biosafety and chemical solubility studies of multiscale crystal-reinforced lithium disilicate glass-ceramics 多尺度晶体增强二硅酸锂玻璃陶瓷的生物安全性和化学溶解性研究。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-08 DOI: 10.1002/jbm.b.35400
Tong Zhang, Jinrong Liu, Jin Qi, Lingxiang Sun, Xiaoming Liu, Jingyu Yan, Yanjie Zhang, Xiuping Wu, Bing Li

Lithium disilicate (Li2Si2O5) glass-ceramics are currently a more widely used all-ceramic restorative material due to their good mechanical properties and excellent aesthetic properties. However, they have a series of problems such as high brittleness and low fracture toughness, which has become the main bottleneck restricting its development. Therefore, in order to compensate for these shortcomings, we propose to prepare a reinforced glass-ceramics with better mechanical properties and to test the biosafety and chemical solubility of the material. Li2Si2O5 whiskers were synthesized by a one-step hydrothermal method, and multi-scale crystal-enhanced Li2Si2O5 glass-ceramics were prepared by reaction sintering. The biosafety of multi-scale crystal-reinforced Li2Si2O5 glass-ceramics was investigated by in vitro cytotoxicity test, rabbit pyrogen test, mice bone marrow micronucleus test, skin sensitization test, sub-chronic systemic toxicity test, and chronic systemic toxicity test. Additionally, the chemical solubility of multi-scale crystal-reinforced Li2Si2O5 glass-ceramics was investigated. The test results showed that the material was non-cytotoxic, non-thermogenic, non-mutagenic, non-sensitizing, and non-systemic. The chemical solubility, determined to be 377 ± 245 μg/cm2, complied with the ISO 6872 standard for the maximum solubility of ceramic materials. Multi-scale crystal-reinforced Li2Si2O5 glass-ceramics' biosafety and chemical solubility met current normative criteria, and they can move on to mechanical property measurements (such as flexural strength test, fatigue life test, friction and wear property study, etc.) and bonding property optimization, which shows promise for future clinical applications.

二硅酸锂(Li2 Si2 O5)玻璃陶瓷因其良好的机械性能和优异的美观性能,是目前应用较为广泛的全瓷修复材料。但其存在脆性大、断裂韧性低等一系列问题,成为制约其发展的主要瓶颈。因此,为了弥补这些不足,我们提出制备一种具有更好机械性能的增强玻璃陶瓷,并对该材料的生物安全性和化学可溶性进行测试。我们采用一步水热法合成了 Li2 Si2 O5 晶须,并通过反应烧结法制备了多尺度晶体增强 Li2 Si2 O5 玻璃陶瓷。通过体外细胞毒性试验、家兔热原试验、小鼠骨髓微核试验、皮肤过敏试验、亚慢性全身毒性试验和慢性全身毒性试验研究了多尺度晶体增强 Li2 Si2 O5 玻璃陶瓷的生物安全性。此外,还研究了多尺度晶体增强 Li2 Si2 O5 玻璃陶瓷的化学溶解性。测试结果表明,该材料无细胞毒性、无致热性、无致突变性、无致敏性、无系统性。化学溶解度为 377 ± 245 μg/cm2,符合 ISO 6872 陶瓷材料最大溶解度标准。多尺度晶体增强 Li2 Si2 O5 玻璃陶瓷的生物安全性和化学溶解度均符合现行规范标准,可进行机械性能测量(如抗弯强度测试、疲劳寿命测试、摩擦和磨损性能研究等)和粘接性能优化,为未来的临床应用带来了希望。
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引用次数: 0
Development and optimization of kaempferol loaded ethosomes using Box–Behnken statistical design: In vitro and ex-vivo assessments 使用 Box-Behnken 统计设计开发和优化山奈酚负载乙素体:体外和体内评估。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-04 DOI: 10.1002/jbm.b.35394
Shraddha Singh Raghav, Bhavna Kumar, Neeraj Kumar Sethiya, Shilpa Pahwa

Kaempferol (KMP) belong to flavonoid class have developed in ethosomal formulation and were evaluated for their potential to treat diabetic foot ulcers. Even though ethosomes are highly deformable, they can pass through human skin intact. KMP ethosomes were formulated using the cold method and optimized by Box–Behnken design (BBD) (three-factor, three-level (33)). The formulation variables used for optimization are drug concentration of KMP, soylecithin content, and ethanol percentage. The optimized formulation was examined using transmission electronic microscopy (TEM), differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy, in-vitro release, ex-vivo permeation studies, and storage stability. The optimized KMP ethosomes was found to have vesicle size (VS) of 283 ± 0.3 nm and zeta potential (ZP) of −29.67 ± 0.3 mV, polydispersity index (PDI) of 0.36, % entrapment efficiency (%EE) of 91.02 ± 0.21%, drug loading (%) of 46.23 ± 2.5% followed by good storage stability at 4°C/60 ± 5% RH. In vitro drug release of optimized KMP ethosomes was 88.2 ± 2.75%, which was approximately double when compared with pure KMP release, that is 49.9 ± 1.89%. The release kinetics for optimized KMP ethosomes follows the Korsmeyer–Peppas model. An apparent permeation coefficient of 356.25 ± 0.5 μg/cm2 was determined and compared with pure KMP (118.46 ± 0.3 μg/cm2) for 24 h. According to the study, ethosomes can be a cutting-edge strategy that offers a new delivery method for prolonged and targeted distribution of KMP in a variety of dosage forms including oral, topical, transdermal, and so forth.

堪非醇(KMP)属于黄酮类化合物,已开发出乙硫体配方,并对其治疗糖尿病足溃疡的潜力进行了评估。尽管乙硫体极易变形,但它们可以完整地通过人体皮肤。KMP 乙素体采用冷制法配制,并通过盒-贝肯设计(BBD)(三因素、三水平(33 ))进行优化。用于优化的配方变量包括 KMP 的药物浓度、卵磷脂含量和乙醇百分比。使用透射电子显微镜(TEM)、差示扫描量热仪(DSC)、傅立叶变换红外光谱(FTIR)、体外释放、体内外渗透研究和储存稳定性对优化配方进行了检测。研究发现,优化后的 KMP 乙硫体的囊泡尺寸(VS)为 283 ± 0.3 nm,ZP 为 -29.67 ± 0.3 mV,多分散指数(PDI)为 0.36,包埋效率(%EE)为 91.02 ± 0.21%,药物负载量(%)为 46.23 ± 2.5%,并且在 4°C/60 ± 5% RH 下具有良好的储存稳定性。经优化的 KMP 乙硫体的体外药物释放率为 88.2 ± 2.75%,与纯 KMP 释放率(49.9 ± 1.89%)相比,约增加了一倍。优化后的 KMP 乙撑体的释放动力学遵循 Korsmeyer-Peppas 模型。与纯 KMP(118.46 ± 0.3 μg/cm2)相比,乙硫体在 24 小时内的表观渗透系数为 356.25 ± 0.5 μg/cm2。 根据这项研究,乙硫体可以作为一种前沿策略,为 KMP 在口服、外用、透皮等多种剂型中的延长和定向分布提供一种新的给药方法。
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引用次数: 0
Evaluation of rabbit adipose derived stem cells fate in perfused multilayered silk fibroin composite scaffold for Osteochondral repair 评估用于骨软骨修复的灌注多层丝纤维素复合支架中兔子脂肪衍生干细胞的命运。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-04 DOI: 10.1002/jbm.b.35396
Sara Zadegan, Bahman Vahidi, Jhamak Nourmohammadi, Asiyeh Shojaee, Nooshin Haghighipour

Development of osteochondral tissue engineering approaches using scaffolds seeded with stem cells in association with mechanical stimulations has been recently considered as a promising technique for the repair of this tissue. In this study, an integrated and biomimetic trilayered silk fibroin (SF) scaffold containing SF nanofibers in each layer was fabricated. The osteogenesis and chondrogenesis of stem cells seeded on the fabricated scaffolds were investigated under a perfusion flow. 3-Dimethylthiazol-2,5-diphenyltetrazolium bromide assay showed that the perfusion flow significantly enhanced cell viability and proliferation. Analysis of gene expression by stem cells revealed that perfusion flow had significantly upregulated the expression of osteogenic and chondrogenic genes in the bone and cartilage layers and downregulated the hypertrophic gene expression in the intermediate layer of the scaffold. In conclusion, applying flow perfusion on the prepared integrated trilayered SF-based scaffold can support osteogenic and chondrogenic differentiation for repairing osteochondral defects.

最近,使用干细胞种子支架结合机械刺激开发骨软骨组织工程方法被认为是修复这种组织的一种有前途的技术。本研究制作了一种集成的仿生物三层丝纤维素(SF)支架,每层都含有SF纳米纤维。在灌注流条件下,研究了播种在所制支架上的干细胞的成骨和软骨发育情况。3-二甲基噻唑-2,5-二苯基溴化四氮唑试验表明,灌注流显著提高了细胞的活力和增殖。干细胞基因表达分析表明,灌注流明显提高了骨层和软骨层的成骨和软骨基因表达,降低了支架中间层的肥大基因表达。总之,在制备好的三层 SF 基集成支架上进行流动灌注可支持成骨和软骨分化,从而修复骨软骨缺损。
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引用次数: 0
Zinc-doped hydroxyapatite as a pH responsive drug delivery system for anticancer drug 6-mercaptopurine 掺锌羟基磷灰石作为抗癌药物 6-巯基嘌呤的 pH 值响应型给药系统。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-04 DOI: 10.1002/jbm.b.35395
Marcel Jakubowski, Łukasz Majchrzycki, Aleksej Zarkov, Adam Voelkel, Mariusz Sandomierski

6-Mercaptopurine (6MP) is commonly used in the treatment of acute lymphoblastic leukemia as an important agent in maintenance therapy. Despite its therapeutic benefits, 6MP has some limitations during therapy. Taking into account the disadvantages during 6MP therapy, there is a great need to create an appropriate delivery system for this drug. 6MP contains in its structure nitrogen and sulfur atoms capable of forming coordination compounds with metal ions, for example zinc. Therefore, in this work, we prepared biocompatible hydroxyapatite (HAp) doped with zinc ions, and used it as a carrier for 6MP. Doped HAp has not been used as a carrier for this drug before. The work proved that the prepared carrier-drug system has a particle size of about 130 nm, which indicates its potential for intravenous delivery. In addition, in an acidic environment (imitating cancer cells), the carrier agglomerates allow targeted release of the drug. The drug is evenly distributed, which indicates that the doses released from it will always be comparable. The release of the drug in a neutral environment is long-lasting in controlled doses, whereas in an acidic environment it is immediate. The obtained results indicate the high potential of the material in both slow-release and cancer-targeted release of 6MP.

6-巯基嘌呤(6MP)是治疗急性淋巴细胞白血病的常用药物,也是维持治疗的重要药物。尽管 6MP 具有治疗效果,但在治疗过程中也存在一些局限性。考虑到 6MP 在治疗过程中的缺点,我们亟需为这种药物开发一种合适的给药系统。6MP 的结构中含有氮和硫原子,能够与金属离子(如锌)形成配位化合物。因此,在这项工作中,我们制备了掺杂锌离子的生物相容性羟基磷灰石(HAp),并将其用作 6MP 的载体。掺杂的 HAp 以前从未用作这种药物的载体。研究证明,所制备的载体-药物系统的粒径约为 130 纳米,这表明它具有静脉注射的潜力。此外,在酸性环境(模仿癌细胞)中,载体团聚体可以定向释放药物。药物分布均匀,这表明其释放的剂量始终相当。在中性环境中,药物的释放在可控剂量下是持久的,而在酸性环境中则是即时的。所获得的结果表明,这种材料在 6MP 的缓释和癌症靶向释放方面都具有很大的潜力。
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引用次数: 0
Development of guar gum reinforced calcium magnesium phosphate-based bone biocement 开发瓜尔胶强化磷酸钙镁骨生物水泥。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-02-24 DOI: 10.1002/jbm.b.35384
Vidul Goenka, Anupama Devi V. K., Ceera Manikandan, Amit Kumar Jaiswal

This study aims at developing a calcium magnesium phosphate-based bone biocement that combines a natural polymer and regenerative properties of bone bonding materials. The formulation of this biocement consists of oxidized guar gum, polydopamine, and calcium magnesium phosphate. The oxidized guar gum is easily soluble in water and has a slightly basic pH, unlike unmodified guar gum, thus allowing a homogenous paste to form in the alkaline environment of calcium magnesium phosphate. Three different oxidized degrees of guar gum were made, and the impact on the biocement properties was studied. The modified guar gum-reinforced biocement (OGG C2) displayed higher mechanical strength and lower degradation rates than OGG B1 and OGG A0. Furthermore, samples with polydopamine exhibited better results, thus, improving the already reinforced biocement. Morphological studies of the biocement displayed a highly porous structure with porosity varying among biocement containing different oxidized guar gum and polydopamine levels.

本研究旨在开发一种基于磷酸钙镁的骨生物水泥,它结合了天然聚合物和骨粘合材料的再生特性。这种生物水泥的配方由氧化瓜尔胶、多巴胺和磷酸钙镁组成。氧化瓜尔胶易溶于水,与未改性瓜尔胶不同,其 pH 值略带碱性,因此能在磷酸钙镁的碱性环境中形成均匀的糊状物。我们制作了三种不同氧化程度的瓜尔胶,并研究了它们对生物水泥性能的影响。与 OGG B1 和 OGG A0 相比,改性瓜尔胶增强生物水泥(OGG C2)显示出更高的机械强度和更低的降解率。此外,含有聚多巴胺的样品显示出更好的效果,从而改善了已增强的生物水泥。生物水泥的形态学研究显示,生物水泥具有高度多孔结构,不同氧化瓜尔胶和多巴胺含量的生物水泥的孔隙率各不相同。
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引用次数: 0
Studying the electrical, mechanical, and biological properties of BCZT–HA composites 研究 BCZT-HA 复合材料的电气、机械和生物特性
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-02-22 DOI: 10.1002/jbm.b.35392
Fatemeh Zare Dehnov, Raziye Hayati, Lobat Tayebi

The piezoelectric properties of natural bone and their influence on bone growth have inspired researchers to study a range of bio-piezoelectric composite materials. By exploring these materials, researchers aim to understand better, how piezoelectricity can be controlled to promote bone growth and tissue regeneration. In this work, the prominent piezoelectric material, (Ba, Zr) TiO3-x(Ba,Ca)TiO3, abbreviated as BCZT, was selected as a possible bone growth enhancer in hydroxyapatite (HA) scaffolds. Initially, BCZT and hydroxyapatite (HA) powders were synthesized using the sol–gel method. Subsequently, various composite samples of BCZT–xHA were prepared using the conventional solid-state method. After sintering the samples at 1300°C, the phase structure, microstructure, density, and electrical properties were characterized. The samples' compressive strength was determined by analyzing the outcomes of basic compression tests. The biological behavior of the samples in terms of in vitro simulated body fluid immersion and MTT tests were evaluated. Our results revealed that among the BCZT–xHA samples, the BCZT-20HA sample had the best composition, considering its electrical, mechanical, and biological properties. A d33 value of 10 pC/N, dielectric permittivity of 110, and the g33 equal to 10.27 mV m/N resulted in the output voltage of 1.03 V. The results of the MTT assay test confirmed the noncytotoxic nature of the samples with the highest optical density in the BCZT-20HA sample.

天然骨骼的压电特性及其对骨骼生长的影响激发了研究人员对一系列生物压电复合材料的研究。通过探索这些材料,研究人员旨在更好地了解如何控制压电性以促进骨骼生长和组织再生。在这项研究中,研究人员选择了著名的压电材料 (Ba, Zr) TiO3-x(Ba,Ca)TiO3(简称 BCZT)作为羟基磷灰石(HA)支架中可能的骨生长促进剂。最初,采用溶胶-凝胶法合成了 BCZT 和羟基磷灰石(HA)粉末。随后,采用传统固态法制备了 BCZT-xHA 的各种复合样品。在 1300°C 下烧结样品后,对其相结构、微观结构、密度和电性能进行了表征。通过分析基本压缩试验的结果,确定了样品的抗压强度。通过体外模拟体液浸泡和 MTT 试验评估了样品的生物学行为。结果表明,在 BCZT-xHA 样品中,BCZT-20HA 样品的电气、机械和生物特性最佳。d33 值为 10 pC/N,介电常数为 110,g33 等于 10.27 mV m/N,输出电压为 1.03 V。MTT 分析测试结果证实了样品的无细胞毒性,其中 BCZT-20HA 样品的光密度最高。
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引用次数: 0
Strontium-loaded 3D intramedullary nail titanium implant for critical-sized femoral defect in rabbits 锶负载三维髓内钉钛植入物治疗兔子临界大小股骨缺损
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-02-22 DOI: 10.1002/jbm.b.35393
Shintaro Honda, Shunsuke Fujibayashi, Takayoshi Shimizu, Seiji Yamaguchi, Yaichiro Okuzu, Yusuke Takaoka, Soichiro Masuda, Mitsuru Takemoto, Toshiyuki Kawai, Bungo Otsuki, Koji Goto, Shuichi Matsuda

The treatment of critical-sized bone defects has long been a major problem for surgeons. In this study, an intramedullary nail shaped three-dimensional (3D)-printed porous titanium implant that is capable of releasing strontium ions was developed through a simple and cost-effective surface modification technique. The feasibility of this implant as a stand-alone solution was evaluated using a rabbit's segmental diaphyseal as a defect model. The strontium-loaded implant exhibited a favorable environment for cell adhesion, and mechanical properties that were commensurate with those of a rabbit's cortical bone. Radiographic, biomechanical, and histological analyses revealed a significantly higher amount of bone ingrowth and superior bone-bonding strength in the strontium-loaded implant when compared to an untreated porous titanium implant. Furthermore, one-year histological observations revealed that the strontium-loaded implant preserved the native-like diaphyseal bone structure without failure. These findings suggest that strontium-releasing 3D-printed titanium implants have the clinical potential to induce the early and efficient repair of critical-sized, load-bearing bone defects.

长期以来,治疗临界大小的骨缺损一直是外科医生面临的一大难题。在这项研究中,通过一种简单而经济有效的表面改性技术,开发出了一种髓内钉形状的三维(3D)打印多孔钛植入物,这种植入物能够释放锶离子。以兔子节段骺端为缺陷模型,评估了这种植入物作为独立解决方案的可行性。锶负载植入物为细胞粘附提供了良好的环境,其机械性能与兔子的皮质骨相当。X光片、生物力学和组织学分析表明,与未经处理的多孔钛种植体相比,锶负载种植体的骨生长量明显增加,骨结合强度也更高。此外,为期一年的组织学观察显示,锶负载种植体保留了类似于原生骨的骺端骨结构,且未出现失效。这些研究结果表明,释放锶的三维打印钛植入物具有临床潜力,可诱导早期、有效地修复临界大小的承重骨缺损。
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Journal of biomedical materials research. Part B, Applied biomaterials
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