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Journal of biomedical materials research. Part B, Applied biomaterials最新文献

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Investigation of morphology and structure of drug-loaded PLA-b-PEO-b-PLA polymeric micelle: A dissipative particle dynamics simulations study 载药聚乳酸-b-PEO-b-PLA 聚合物胶束的形态和结构研究:耗散粒子动力学模拟研究。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-05-10 DOI: 10.1002/jbm.b.35410
Dongmei Liu, Ye Lin, Danping Wang, Yongchao Jin, Kai Gong

The dissipative particle dynamics (DPD) simulation was used to study the morphologies and structures of the paclitaxel-loaded PLA-b-PEO-b-PLA polymeric micelle. We focused on the influences of PLA block length, PLA-b-PEO-b-PLA copolymer concentration, paclitaxel drug content on morphologies and structures of the micelle. Our simulations show that: (i) with the PLA block length increase, the self-assemble structure of PLA-b-PEO-b-PLA copolymers with paclitaxel vary between onion-like structure (core-middle layer-shell) to spherical core-shell structure. The PEO shell thins and the size of the PLA core increases. The onionlike structures are comprised of the PEO hydrophilic core, the PLA hydrophobic middle layer, and the PEO hydrophilic shell, the distribution of the paclitaxel drug predominantly occurs within the hydrophobic intermediate layer; (ii) The system forms a spherical core-shell structure when a small amount of the drug is added, and within a certain range, the size of the spherical structure increases as the drug amount increases. When the drug contents (volume fraction) cdrug = 10%, it can be observed that the PLA4-b-PEO19-b-PLA4 spherical structures connect to form rod-shaped structures. With the length of PLA block NPLA = 8, as the paclitaxel drug concentrations cdrug = 4%, PEO has been insufficient to completely encapsulate the PLA and paclitaxel drug beads. To enhance drug loading capacity while maintaining stability of the system in aqueous solution, the optimal composition for loading paclitaxel is PLA4-b-PEO19-b-PLA4; the drug content is not higher than 4%; (iii) The paclitaxel-loaded PLA4-b-PEO19-b-PLA4 micelle undergo the transition from onionlike (core-middle layer-shell) to spherical (core-shell) to rod-shaped and lamellar structure as the PLA4-b-PEO19-b-PLA4 copolymer concentration increases from ccp = 10% to 40%.

利用耗散粒子动力学(DPD)模拟研究了负载紫杉醇的聚乳酸-b-PEO-b-PLA聚合物胶束的形态和结构。我们重点研究了聚乳酸嵌段长度、聚乳酸-b-PEO-b-PLA 共聚物浓度、紫杉醇药物含量对胶束形态和结构的影响。模拟结果表明(i) 随着聚乳酸嵌段长度的增加,PLA-b-PEO-b-PLA 共聚物与紫杉醇的自组装结构在洋葱状结构(核-中间层-壳)和球形核-壳结构之间变化。PEO 外壳变薄,PLA 核心的尺寸增大。洋葱状结构由 PEO 亲水核、PLA 疏水中间层和 PEO 亲水壳组成,紫杉醇药物主要分布在疏水中间层;(ii) 当加入少量药物时,体系形成球形核壳结构,在一定范围内,球形结构的尺寸随着药物量的增加而增大。当药物含量(体积分数)cdrug = 10%时,可以观察到 PLA4-b-PEO19-b-PLA4 球形结构连接形成杆状结构。当聚乳酸块的长度 NPLA = 8 时,紫杉醇药物浓度 cdrug = 4%,PEO 已不足以完全包裹聚乳酸和紫杉醇药物珠。为了提高药物负载能力,同时保持体系在水溶液中的稳定性,负载紫杉醇的最佳组成为 PLA4-b-PEO19-b-PLA4,药物含量不高于 4%;(iii) 随着 PLA4-b-PEO19-b-PLA4 共聚物浓度从 ccp = 10% 增加到 40%,负载紫杉醇的 PLA4-b-PEO19-b-PLA4 胶束会从洋葱状(核-中间层-壳)过渡到球状(核-壳),再过渡到棒状和片状结构。
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引用次数: 0
GelMA-based hydrogel biomaterial scaffold: A versatile platform for regenerative endodontics 基于 GelMA 的水凝胶生物材料支架:用于牙髓再生的多功能平台
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-05-03 DOI: 10.1002/jbm.b.35412
Lei Huang, Xuan Chen, XiaoXia Yang, Yinchun Zhang, Xiaoling Qiu

Endodontic therapy, while generally successful, is primarily limited to mature teeth, hence the pressing need to explore regenerative approaches. Gelatin methacryloyl (GelMA) hydrogels have emerged as pivotal biomaterials, promising a bright future for dental pulp regeneration. Despite advancements in tissue engineering and biomaterials, achieving true pulp tissue regeneration remains a formidable task. GelMA stands out for its injectability, rapid gelation, and excellent biocompatibility, serving as the cornerstone of scaffold materials. In the pursuit of dental pulp regeneration, GelMA holds significant potential, facilitating the delivery of stem cells, growth factors, and other vital substances crucial for tissue repair. Presently, in the field of dental pulp regeneration, researchers have been diligently utilizing GelMA hydrogels as engineering scaffolds to transport various effective substances to promote pulp regeneration. However, existing research is relatively scattered and lacks comprehensive reviews and summaries. Therefore, the primary objective of this article is to elucidate the application of GelMA hydrogels as regenerative scaffolds in this field, thereby providing clear direction for future researchers. Additionally, this article provides a comprehensive discussion on the synthesis, characterization, and application of GelMA hydrogels in root canal therapy regeneration. Furthermore, it offers new application strategies and profound insights into future challenges, such as optimizing GelMA formulations to mimic the complex microenvironment of pulp tissue and enhancing its integration with host tissues.

牙髓治疗虽然普遍成功,但主要局限于成熟的牙齿,因此迫切需要探索再生方法。明胶甲基丙烯酰(GelMA)水凝胶已成为举足轻重的生物材料,有望为牙髓再生带来光明的前景。尽管组织工程和生物材料取得了进步,但实现真正的牙髓组织再生仍然是一项艰巨的任务。GelMA 因其注射性、快速凝胶化和出色的生物相容性而脱颖而出,成为支架材料的基石。在追求牙髓再生的过程中,GelMA 具有巨大的潜力,可促进干细胞、生长因子和其他对组织修复至关重要的物质的输送。目前,在牙髓再生领域,研究人员一直在努力利用 GelMA 水凝胶作为工程支架,输送各种有效物质以促进牙髓再生。然而,现有的研究相对分散,缺乏全面的综述和总结。因此,本文的主要目的是阐明 GelMA 水凝胶作为再生支架在该领域的应用,从而为未来的研究人员提供明确的方向。此外,本文还全面探讨了 GelMA 水凝胶的合成、表征以及在根管治疗再生中的应用。此外,文章还提供了新的应用策略和对未来挑战的深刻见解,例如优化 GelMA 配方以模拟牙髓组织复杂的微环境并增强其与宿主组织的整合。
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引用次数: 0
Improving material properties of a poloxamer P407 hydrogel-based hydroxyapatite bone substitute material by adding silica—A comparative in vivo study 通过添加二氧化硅改善以 poloxamer P407 水凝胶为基础的羟基磷灰石骨替代材料的材料性能--体内对比研究
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-05-03 DOI: 10.1002/jbm.b.35405
Peer W. Kämmerer, Diana Heimes, Franziska Zaage, Cornelia Ganz, Bernhard Frerich, Thomas Gerber, Michael Dau

The structure and handling properties of a P407 hydrogel-based bone substitute material (BSM) might be affected by different poloxamer P407 and silicon dioxide (SiO2) concentrations. The study aimed to compare the mechanical properties and biological parameters (bone remodeling, BSM degradation) of a hydroxyapatite: silica (HA)-based BSM with various P407 hydrogels in vitro and in an in vivo rat model. Rheological analyses for mechanical properties were performed on one BSM with an SiO2-enriched hydrogel (SPH25) as well on two BSMs with unaltered hydrogels in different gel concentrations (PH25 and PH30). Furthermore, the solubility of all BSMs were tested. In addition, 30 male Wistar rats underwent surgical creation of a well-defined bone defect in the tibia. Defects were filled randomly with PH30 (n = 15) or SPH25 (n = 15). Animals were sacrificed after 12 (n = 5 each), 21 (n = 5 each), and 63 days (n = 5 each). Histological evaluation and histomorphometrical quantification of new bone formation (NB;%), residual BSM (rBSM;%), and soft tissue (ST;%) was conducted. Rheological tests showed an increased viscosity and lower solubility of SPH when compared with the other hydrogels. Histomorphometric analyses in cancellous bone showed a decrease of ST in PH30 (p = .003) and an increase of NB (PH30: p = .001; SPH: p = .014) over time. A comparison of both BSMs revealed no significant differences. The addition of SiO2 to a P407 hydrogel-based hydroxyapatite BSM improves its mechanical stability (viscosity, solubility) while showing similar in vivo healing properties compared to PH30. Additionally, the SiO2-enrichment allows a reduction of poloxamer ratio in the hydrogel without impairing the material properties.

基于 P407 水凝胶的骨替代材料(BSM)的结构和处理特性可能会受到不同聚氧乙烯聚酰胺 P407 和二氧化硅(SiO2)浓度的影响。本研究旨在比较羟基磷灰石:二氧化硅(HA)基 BSM 与各种 P407 水凝胶在体外和大鼠体内模型中的机械性能和生物参数(骨重塑、BSM 降解)。对一种使用富含二氧化硅水凝胶的 BSM(SPH25)以及两种使用不同凝胶浓度的未改变水凝胶的 BSM(PH25 和 PH30)进行了机械性能流变学分析。此外,还测试了所有 BSM 的溶解度。此外,还对 30 只雄性 Wistar 大鼠的胫骨进行了明确骨缺损手术。缺损处随机填充 PH30(n = 15)或 SPH25(n = 15)。动物分别在 12 天(5 只)、21 天(5 只)和 63 天(5 只)后牺牲。对新骨形成(NB;%)、残余 BSM(rBSM;%)和软组织(ST;%)进行组织学评估和组织形态计量。流变学测试表明,与其他水凝胶相比,SPH 的粘度更高,溶解度更低。松质骨的组织形态分析表明,随着时间的推移,PH30 的 ST 值下降(p = 0.003),而 NB 值上升(PH30:p = 0.001;SPH:p = 0.014)。两种 BSM 的比较结果显示没有明显差异。与 PH30 相比,在基于 P407 水凝胶的羟基磷灰石 BSM 中添加 SiO2 可提高其机械稳定性(粘度、溶解性),同时显示出类似的体内愈合特性。此外,二氧化硅的富集允许降低水凝胶中的聚氧乙烯醚比例,而不会影响材料的特性。
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引用次数: 0
Biodegradable electrospun poly(L-lactide-co-ε-caprolactone)/polyethylene glycol/bioactive glass composite scaffold for bone tissue engineering 用于骨组织工程的生物可降解电纺聚(L-内酯-共ε-己内酯)/聚乙二醇/生物活性玻璃复合支架
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-04-27 DOI: 10.1002/jbm.b.35406
Joyce R. de Souza, Lais M. Cardoso, Priscila T. A. de Toledo, Maedeh Rahimnejad, Letícia T. Kito, Gilmar P. Thim, Tiago M. B. Campos, Alexandre L. S. Borges, Marco C. Bottino

The field of tissue engineering has witnessed significant advancements in recent years, driven by the pursuit of innovative solutions to address the challenges of bone regeneration. In this study, we developed an electrospun composite scaffold for bone tissue engineering. The composite scaffold is made of a blend of poly(L-lactide-co-ε-caprolactone) (PLCL) and polyethylene glycol (PEG), with the incorporation of calcined and lyophilized silicate-chlorinated bioactive glass (BG) particles. Our investigation involved a comprehensive characterization of the scaffold's physical, chemical, and mechanical properties, alongside an evaluation of its biological efficacy employing alveolar bone-derived mesenchymal stem cells. The incorporation of PEG and BG resulted in elevated swelling ratios, consequently enhancing hydrophilicity. Thermal gravimetric analysis confirmed the efficient incorporation of BG, with the scaffolds demonstrating thermal stability up to 250°C. Mechanical testing revealed enhanced tensile strength and Young's modulus in the presence of BG; however, the elongation at break decreased. Cell viability assays demonstrated improved cytocompatibility, especially in the PLCL/PEG+BG group. Alizarin red staining indicated enhanced osteoinductive potential, and fluorescence analysis confirmed increased cell adhesion in the PLCL/PEG+BG group. Our findings suggest that the PLCL/PEG/BG composite scaffold holds promise as an advanced biomaterial for bone tissue engineering.

近年来,组织工程领域取得了重大进展,其驱动力是寻求创新解决方案来应对骨再生的挑战。在这项研究中,我们开发了一种用于骨组织工程的电纺复合支架。该复合支架由聚(L-乳酸-ε-己内酯)(PLCL)和聚乙二醇(PEG)混合制成,并加入了煅烧和冻干的硅酸盐氯化生物活性玻璃(BG)颗粒。我们的研究包括对支架的物理、化学和机械特性进行全面鉴定,同时利用肺泡骨源性间充质干细胞对其生物功效进行评估。PEG 和 BG 的加入提高了溶胀率,从而增强了亲水性。热重力分析证实了 BG 的有效掺入,支架具有高达 250°C 的热稳定性。机械测试表明,在含有 BG 的情况下,拉伸强度和杨氏模量均有所提高;但断裂伸长率有所下降。细胞活力测试表明,细胞相容性得到改善,尤其是 PLCL/PEG+BG 组。茜素红染色表明骨诱导潜能增强,荧光分析证实 PLCL/PEG+BG 组的细胞粘附性增强。我们的研究结果表明,PLCL/PEG/BG 复合支架有望成为骨组织工程的先进生物材料。
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引用次数: 0
The crystallization properties of antifreeze GelMA hydrogel and its application in cryopreservation of tissue-engineered skin constructs 抗冻 GelMA 水凝胶的结晶特性及其在组织工程皮肤构建物低温保存中的应用
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-04-27 DOI: 10.1002/jbm.b.35408
Jia Tan, Jiahui Li, Xinli Zhou

Gelatin methacrylate (GelMA) hydrogels are expected to be ideal skin tissue engineering dressings for a wide range of clinical treatments. Herein, we report the preparation of GelMA or antifreeze GelMA hydrogel sheets with different GelMA concentrations, crosslinking times, and cryoprotectant (CPA) concentrations. The crystallization properties of GelMA or antifreeze GelMA hydrogel sheets were studied by cryomicroscopy and differential scanning calorimetry (DSC). It was found that the growth of ice crystals was slower when GelMA hydrogel concentration was more than 7%. The 10% DMSO-7% GelMA hydrogel sheets crosslinked for 60 min showed no ice crystal formation and growth during cooling and warming. The DSC results showed that the vitrification temperature of the 10% DMSO-7% GelMA hydrogel sheet was −111°C. Furthermore, slow freezing and rapid freezing of fibroblast-laden GelMA or antifreeze GelMA hydrogel sheets, and tissue-engineered skin constructs were studied. The results showed no significant difference in cell survival between slow (88.8% ± 1.51) and rapid (89.2% ± 3.00) freezing of fibroblast-loaded 10% DMSO-7% GelMA hydrogel sheets, and significantly higher than that of 7% GelMA hydrogel sheets (33.4% ± 5.46). The cell viability was higher in tissue-engineered skin constructs after slow freezing (86.34% ± 1.45) than rapid freezing (72.74% ± 1.34). We believe that the combination of antifreeze hydrogels and tissue engineering will facilitate the cryopreservation of tissue engineering constructs.

甲基丙烯酸明胶(GelMA)水凝胶有望成为用于各种临床治疗的理想皮肤组织工程敷料。在此,我们报告了不同 GelMA 浓度、交联时间和低温保护剂(CPA)浓度下 GelMA 或抗冻 GelMA 水凝胶片材的制备过程。通过冷冻显微镜和差示扫描量热法(DSC)研究了 GelMA 或抗冻 GelMA 水凝胶片的结晶特性。结果发现,当 GelMA 水凝胶的浓度超过 7% 时,冰晶的生长速度较慢。交联 60 分钟的 10% DMSO-7% GelMA 水凝胶片材在冷却和升温过程中没有冰晶形成和生长。DSC 结果表明,10% DMSO-7% GelMA 水凝胶片材的玻璃化温度为 -111°C 。此外,还研究了含有成纤维细胞的 GelMA 或抗冻 GelMA 水凝胶片材以及组织工程皮肤构建物的缓慢冷冻和快速冷冻。结果表明,缓慢冷冻(88.8% ± 1.51)和快速冷冻(89.2% ± 3.00)含成纤维细胞的 10% DMSO-7% GelMA 水凝胶片材的细胞存活率无明显差异,但显著高于含 7% GelMA 水凝胶片材的细胞存活率(33.4% ± 5.46)。慢速冷冻(86.34% ± 1.45)后组织工程皮肤构建体的细胞存活率高于快速冷冻(72.74% ± 1.34)。我们相信,防冻水凝胶与组织工程的结合将有助于组织工程构建物的冷冻保存。
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引用次数: 0
Bonding of ceramics to silver-coated titanium—A combined theoretical and experimental study 陶瓷与银涂层钛的结合--理论与实验的综合研究
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-04-27 DOI: 10.1002/jbm.b.35407
Vesa Vuorinen, Reijo Kouhia, Mauno Könönen, Jorma K. Kivilahti

It would be very beneficial to have a method for joining of ceramics to titanium reliably. Although several techniques have been developed and tested to prevent extensive interfacial chemical reactions in titanium-ceramic systems, the main problem of the inherent brittleness of interfaces was still unsolved. To overcome this problem also in dental applications, we decided to make use of an interlayer material that needs to meet the following requirements: First, it has to be biocompatible, second, it should not melt below the bonding temperatures, and third, it should not react too strongly with titanium, so that its plasticity will be maintained. Considering possible material options only the metals: gold, platinum, palladium, and silver, fulfill the first and second requirements. To find out—without an extensive experimental testing program—which of the four metals fulfills the third requirement best, the combined thermodynamic and reaction kinetic modeling was employed to evaluate how many and how thick reaction layers are formed between the interlayer metals and titanium. With the help of theoretical modeling, it was shown that silver fulfills the last requirement best. However, before starting to test experimentally the effect of the silver layer on the mechanical integrity of dental ceramic/Ag/Ti joints it was decided to make use of mechanical analysis of the three-point bending test, the result of which indicated that the silver layer increases significantly the bond strength of the joints. This result encouraged us to develop a new technique for plating silver on titanium. Subsequently, we executed numerous three-point bending tests, which demonstrated that silver-plated titanium-ceramic joints are much stronger than conventional titanium-ceramic joints. Hence, it can be concluded that the combined thermodynamic, reaction kinetic, and mechanical modeling method can also be a very valuable tool in medical research and development work.

将陶瓷与钛可靠地连接在一起的方法是非常有益的。虽然已经开发并测试了几种技术来防止钛陶瓷系统中广泛的界面化学反应,但界面固有的脆性这一主要问题仍未得到解决。为了在牙科应用中克服这一问题,我们决定使用一种需要满足以下要求的夹层材料:首先,它必须具有生物兼容性;其次,它在粘合温度以下不会熔化;第三,它与钛的反应不会太强烈,以保持钛的可塑性。考虑到可能的材料选择,只有金、铂、钯和银等金属能满足第一和第二项要求。为了在不进行大量实验测试的情况下找出四种金属中哪一种最符合第三项要求,我们采用了热力学和反应动力学相结合的模型来评估层间金属和钛之间形成的反应层的数量和厚度。在理论模型的帮助下,结果表明银最符合最后一项要求。然而,在开始实验测试银层对牙科陶瓷/搀/钛接头机械完整性的影响之前,我们决定利用三点弯曲试验的机械分析,结果表明银层能显著提高接头的粘接强度。这一结果促使我们开发出一种在钛上镀银的新技术。随后,我们又进行了多次三点弯曲试验,结果表明镀银钛陶瓷接头的强度远远高于传统的钛陶瓷接头。因此,可以得出结论,热力学、反应动力学和机械建模相结合的方法在医学研究和开发工作中也是一种非常有价值的工具。
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引用次数: 0
Albumin suppresses oxidation of TiNb alloy in the simulated inflammatory environment 白蛋白可抑制模拟炎症环境中 TiNb 合金的氧化。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-27 DOI: 10.1002/jbm.b.35404
Agata Sotniczuk, Damian Kalita, Witold Chromiński, Magdalena Matczuk, Marcin Pisarek, Halina Garbacz

Literature data has shown that reactive oxygen species (ROS), generated by immune cells during post-operative inflammation, could induce corrosion of standard Ti-based biomaterials. For Ti6Al4V alloy, this process can be further accelerated by the presence of albumin. However, this phenomenon remains unexplored for Ti β-phase materials, such as TiNb alloys. These alloys are attractive due to their relatively low elastic modulus value. This study aims to address the question of how albumin influences the corrosion resistance of TiNb alloy under simulated inflammation. Electrochemical and ion release tests have revealed that albumin significantly enhances corrosion resistance over both short (2 and 24 h) and long (2 weeks) exposure periods. Furthermore, post-immersion XPS and cross-section TEM analysis have demonstrated that prolonged exposure to an albumin-rich inflammatory solution results in the complete coverage of the TiNb surface by a protein layer. Moreover, TEM studies revealed that H2O2-induced oxidation and further formation of a defective oxide film were suppressed in the solution enriched with albumin. Overall results indicate that contrary to Ti6Al4V, the addition of albumin to the PBS + H2O2 solution is not necessary to simulate the harsh inflammatory conditions as could possibly be found in the vicinity of a TiNb implant.

文献数据显示,术后炎症期间免疫细胞产生的活性氧(ROS)可诱发标准钛基生物材料的腐蚀。对于 Ti6Al4V 合金,白蛋白的存在会进一步加速这一过程。然而,对于 Ti β 相材料(如 TiNb 合金)来说,这一现象仍未得到研究。这些合金因其相对较低的弹性模量值而颇具吸引力。本研究旨在解决白蛋白在模拟炎症条件下如何影响 TiNb 合金耐腐蚀性的问题。电化学和离子释放测试表明,白蛋白在短时间(2 小时和 24 小时)和长时间(2 周)暴露下都能显著增强耐腐蚀性。此外,浸泡后的 XPS 和横截面 TEM 分析表明,长期暴露于富含白蛋白的炎症溶液中会导致蛋白质层完全覆盖 TiNb 表面。此外,TEM 研究表明,在富含白蛋白的溶液中,H2O2 诱导的氧化和缺陷氧化膜的进一步形成受到抑制。总体结果表明,与 Ti6Al4V 相反,在 PBS + H2O2 溶液中添加白蛋白并不是模拟 TiNb 植入体附近可能出现的严酷炎症条件所必需的。
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引用次数: 0
Emulgel based on fish skin collagen-microalgae-silver increased neovascularization and re-epithelialization of full thickness burn in rats 基于鱼皮胶原-微藻-银的 Emulgel 增加了大鼠全厚度烧伤的新生血管和再上皮化。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-27 DOI: 10.1002/jbm.b.35399
Francisco Fábio Pereira de Souza, Igor Iuco Castro-Silva, Fábia Karine Andrade, Adriano Lincoln Albuquerque Mattos, Mirrael de Sousa Lopes, Wallady da Silva Barroso, Bartolomeu Warlene Silva de Souza, Men de Sá Moreira de Souza-Filho, André Luis Coelho da Silva

Deep skin burn represents a global morbidity and mortality problem, and the limitation of topical treatment agents has motivated research to development new formulations capable of preventing infections and accelerating healing. The aim of this work was to develop and characterize an emulgel based on collagen (COL) and gelatin (GEL) extracted from fish skin associated with Chlorella vulgaris extract (CE) and silver nitrate (AgNO3). COL and GEL were characterized by physicochemical and thermal analyses; and CE by electrophoresis and its antioxidant capacity. Three emulgels formulations were developed: COL (0.5%) + GEL (2.5%) (E1), COL+GEL+CE (1%) (E2), and COL+GEL+CE + AgNO3 (0.1%) (E3). All formulations were characterized by physicochemical, rheology assays, and preclinical analyses: cytotoxicity (in vitro) and healing potential using a burn model in rats. COL and GEL showed typical physicochemical characteristics, and CE presented 1.3 mg/mL of proteins and antioxidant activity of 76%. Emulgels presented a coherent physicochemical profile and pseudoplastic behavior. Preclinical analysis showed concentration-dependent cytotoxicity against fibroblast and keratinocytes. In addition, all emulgels induced similar percentages of wound contraction and complete wound closure in 28 days. The histopathological analysis showed higher scores for polymorphonuclear cells to E1 and greater neovascularization and re-epithelialization to E3. Then, E3 formulation has potential to improve burn healing, although its use in a clinical setting requires further studies.

皮肤深度烧伤是一个全球性的发病率和死亡率问题,由于局部治疗药物的局限性,促使人们研究开发能够预防感染和加速愈合的新配方。这项工作的目的是开发一种基于从鱼皮中提取的胶原蛋白(COL)和明胶(GEL),并与小球藻提取物(CE)和硝酸银(AgNO3)结合的凝胶,并对其进行表征。对胶原蛋白和明胶进行了物理化学和热分析,对胶原蛋白进行了电泳分析并测定了其抗氧化能力。共开发出三种乳凝胶配方:COL(0.5%)+ GEL(2.5%)(E1)、COL+GEL+CE(1%)(E2)和 COL+GEL+CE + AgNO3(0.1%)(E3)。所有配方都通过理化、流变学检测和临床前分析进行了表征:细胞毒性(体外)和使用大鼠烧伤模型的愈合潜力。COL和GEL显示出典型的物理化学特征,CE显示出1.3毫克/毫升的蛋白质和76%的抗氧化活性。Emulgels具有一致的物理化学特征和假塑性行为。临床前分析表明,它对成纤维细胞和角质细胞具有浓度依赖性细胞毒性。此外,所有的凝胶都能诱导相似比例的伤口收缩,并在 28 天内使伤口完全闭合。组织病理学分析表明,E1 的多形核细胞得分更高,而 E3 的新生血管和再上皮化程度更高。因此,E3 配方具有改善烧伤愈合的潜力,但其在临床上的应用还需进一步研究。
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引用次数: 0
Photocurable extracellular matrix sealant for cessation of venous hemorrhage 用于终止静脉出血的光固化细胞外基质密封剂。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-23 DOI: 10.1002/jbm.b.35401
Luke E. Schepers, Brooke L. Martindale, Alycia G. Berman, Hannah L. Cebull, William Van Alstine, Sydney E. Hollingshead, Tyler Novak, Craig J. Goergen

Hemorrhage is the second leading cause of death in patients under 46 years of age in the United States. Cessation of hemorrhage prevents hemorrhagic shock and tissue hypoxia. Controlling the bleed via direct pressure or tourniquet is often the first line of defense, but long-term care requires staples, hemostatic agents, or sealants that seal the vessel and restore blood flow. Here, we compare a new photocurable extracellular matrix sealant (pcECM) with low, medium, and high crosslink density formulations to a commercially available fibrin-based sealant, TISSEEL®. pcECM has potential uses in surgical and remote settings due to room temperature storage conditions and fast preparation time. Here, we determine if pcECM sealant can stop venous hemorrhage in a murine model, adhere to the wound site in vivo throughout the wound-healing process, and has the mechanical properties necessary for stopping hemorrhage. Adjusting pcECM crosslinking density significantly affected viscosity, swelling, burst strength, tensile strength, and elasticity of the sealant. 3-Dimensional ultrasound volume segmentations showed pcECM degrades to 17 ± 8% of its initial implant volume by day 28. Initially, local hemodynamic changes were observed, but returned close to baseline levels by day 28. Acute inflammation was observed near the puncture site in pcECM implanted mice, and we observed inflammatory markers at the 14-day explant for both sealants. pcECM and fibrin sealant successfully sealed the vessel in all cases, and consistently degraded over 14–28 days. pcECM is a durable sealant with tunable mechanical properties and possible uses in hemorrhage control and other surgical procedures.

出血是美国 46 岁以下患者的第二大死因。止血可防止失血性休克和组织缺氧。通过直接加压或止血带控制出血通常是第一道防线,但长期护理需要用订书机、止血剂或密封剂来密封血管并恢复血流。在这里,我们将一种新型光固化细胞外基质密封剂(pcECM)的低、中、高交联密度配方与市售的纤维蛋白基密封剂 TISSEEL® 进行了比较。pcECM 具有室温储存条件和快速制备时间,可用于外科手术和偏远地区。在此,我们将确定 pcECM 密封剂是否能在小鼠模型中止住静脉出血,是否能在整个伤口愈合过程中粘附在伤口部位,以及是否具有止血所需的机械特性。调整 pcECM 交联密度可显著影响密封剂的粘度、膨胀、爆破强度、拉伸强度和弹性。三维超声体积分割显示,pcECM 在第 28 天时降解到初始植入体积的 17 ± 8%。最初观察到局部血流动力学变化,但到第 28 天又恢复到接近基线水平。pcECM 和纤维蛋白密封剂在所有病例中都成功地密封了血管,并在 14-28 天内持续降解。pcECM 是一种耐用的密封剂,具有可调的机械特性,可用于出血控制和其他外科手术。
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引用次数: 0
Direct inkjet writing of polylactic acid/β-tricalcium phosphate composites for bone tissue regeneration: A proof-of-concept study 用于骨组织再生的聚乳酸/β-磷酸三钙复合材料的直接喷墨写入:概念验证研究。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-03-23 DOI: 10.1002/jbm.b.35402
Vasudev Vivekanand Nayak, Vijayavenkataraman Sanjairaj, Rakesh Kumar Behera, James E. Smay, Nikhil Gupta, Paulo G. Coelho, Lukasz Witek

There is an ever-evolving need of customized, anatomic-specific grafting materials for bone regeneration. More specifically, biocompatible and osteoconductive materials, that may be configured dynamically to fit and fill defects, through the application of an external stimulus. The objective of this study was to establish a basis for the development of direct inkjet writing (DIW)-based shape memory polymer-ceramic composites for bone tissue regeneration applications and to establish material behavior under thermomechanical loading. Polymer-ceramic (polylactic acid [PLA]/β-tricalcium phosphate [β-TCP]) colloidal gels were prepared of different w/w ratios (90/10, 80/20, 70/30, 60/40, and 50/50) through polymer dissolution in acetone (15% w/v). Cytocompatibility was analyzed through Presto Blue assays. Rheological properties of the colloidal gels were measured to determine shear-thinning capabilities. Gels were then extruded through a custom-built DIW printer. Space filling constructs of the gels were printed and subjected to thermomechanical characterization to measure shape fixity (Rf) and shape recovery (Rr) ratios through five successive shape memory cycles. The polymer-ceramic composite gels exhibited shear-thinning capabilities for extrusion through a nozzle for DIW. A significant increase in cellular viability was observed with the addition of β-TCP particles within the polymer matrix relative to pure PLA. Shape memory effect in the printed constructs was repeatable up to 4 cycles followed by permanent deformation. While further research on scaffold macro-/micro-geometries, and engineered porosities are warranted, this proof-of-concept study suggested suitability of this polymer-ceramic material and the DIW 3D printing workflow for the production of customized, patient specific constructs for bone tissue engineering.

人们对用于骨再生的定制化、解剖特异性移植材料的需求不断增长。更具体地说,生物相容性和骨传导性材料可以通过施加外部刺激进行动态配置,以适应和填补缺损。本研究的目的是为开发基于直接喷墨书写(DIW)的形状记忆聚合物陶瓷复合材料奠定基础,以用于骨组织再生应用,并确定材料在热机械负载下的行为。通过在丙酮(15% w/v)中溶解聚合物,制备了不同重量/重量比(90/10、80/20、70/30、60/40 和 50/50)的聚合物-陶瓷(聚乳酸 [PLA] /β-磷酸三钙 [β-TCP])胶体凝胶。细胞相容性通过 Presto Blue 试验进行分析。测量胶体凝胶的流变特性以确定剪切稀化能力。然后通过定制的 DIW 打印机挤出凝胶。打印凝胶的空间填充结构,并对其进行热力学表征,通过五个连续的形状记忆循环来测量形状固定率(Rf)和形状恢复率(Rr)。聚合物-陶瓷复合凝胶具有剪切稀化能力,可通过喷嘴挤出用于 DIW。与纯聚乳酸相比,在聚合物基质中添加β-TCP颗粒后,细胞活力明显提高。打印构建物中的形状记忆效应可重复达 4 个周期,随后出现永久变形。虽然还需要对支架的宏观/微观几何形状和工程孔隙率进行进一步研究,但这项概念验证研究表明,这种聚合物陶瓷材料和 DIW 三维打印工作流程适用于生产定制的、患者专用的骨组织工程构建体。
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引用次数: 0
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Journal of biomedical materials research. Part B, Applied biomaterials
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