Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.015
Qifang Cai, Ke Jin, Tian-hui Wu, Y. Yi, Jie Nie
Objective To investigate the study of magnetic resonance (MR) diffusion tensor imaging in children with acute kidney injury, and further improve the clinical research level of early diagnosis of acute kidney injury (AKI). Methods Twenty-two children who met the clinical AKI diagnostic criteria were collected from the Children's Hospital of Hunan Province. Twenty-three children volunteers were collected as the control group. The anisotropy fraction (FA) and mean diffusion coefficient (ADC) values of the renal cortex and medulla of all the tested children were detected, and the serum creatinine value and disease test results of children with AKI were collected. Spearman correlation analysis was used to analyze the correlation between the renal and medullary ADC values and FA values and serum creatinine values in the children with AKI. Results There were no significant differences in the FA, ADC values of left and right renal cortex and medulla of case group (P>0.05). There were no significant differences in the FA, ADC values of left and right renal cortex and medulla of control group (P>0.05). The medullary FA value, cortical FA and ADC value of the children with AKI were significantly lower than those of normal children (P 0.05). The medullary FA value and cortical ADC value of AKI patients were negatively correlated with serum creatinine value (r=-0.868, -0.436, P<0.05), and there was no correlation between cortical FA, medullary ADC and serum creatinine in the rest of the children. Conclusions As a non-invasive imaging method, diffusion tensor imaging (DTI) can reflect the early renal damage of AKI and has potential application value for clinical diagnosis of AKI. Key words: Diffusion tensor imaging; Magnetic resonance imaging; Acute kidney injury; Child
{"title":"Preliminary evaluation of MR diffusion tensor imaging in children with acute renal injury","authors":"Qifang Cai, Ke Jin, Tian-hui Wu, Y. Yi, Jie Nie","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.015","url":null,"abstract":"Objective \u0000To investigate the study of magnetic resonance (MR) diffusion tensor imaging in children with acute kidney injury, and further improve the clinical research level of early diagnosis of acute kidney injury (AKI). \u0000 \u0000 \u0000Methods \u0000Twenty-two children who met the clinical AKI diagnostic criteria were collected from the Children's Hospital of Hunan Province. Twenty-three children volunteers were collected as the control group. The anisotropy fraction (FA) and mean diffusion coefficient (ADC) values of the renal cortex and medulla of all the tested children were detected, and the serum creatinine value and disease test results of children with AKI were collected. Spearman correlation analysis was used to analyze the correlation between the renal and medullary ADC values and FA values and serum creatinine values in the children with AKI. \u0000 \u0000 \u0000Results \u0000There were no significant differences in the FA, ADC values of left and right renal cortex and medulla of case group (P>0.05). There were no significant differences in the FA, ADC values of left and right renal cortex and medulla of control group (P>0.05). The medullary FA value, cortical FA and ADC value of the children with AKI were significantly lower than those of normal children (P 0.05). The medullary FA value and cortical ADC value of AKI patients were negatively correlated with serum creatinine value (r=-0.868, -0.436, P<0.05), and there was no correlation between cortical FA, medullary ADC and serum creatinine in the rest of the children. \u0000 \u0000 \u0000Conclusions \u0000As a non-invasive imaging method, diffusion tensor imaging (DTI) can reflect the early renal damage of AKI and has potential application value for clinical diagnosis of AKI. \u0000 \u0000 \u0000Key words: \u0000Diffusion tensor imaging; Magnetic resonance imaging; Acute kidney injury; Child","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90334508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.017
Lihua Pan, F. Zhou, Y. Ke
Objective To analyze the changes of Survivin, MSH6 and MSH2 expression in colorectal cancer and explore their relationship with clinical and pathological parameters. Methods 197 cases of colorectal adenocarcinoma and 20 cases of inflammatory intestinal mucosa were detected by immunohistochemistry with Survivin, MSH6 and MSH2, and the correlation between Survivin, MSH6 and MSH2 expression was analyzed. Results In the control group of 20 cases with inflammatory non-tumor intestinal mucosa, the positive expression rate of MSH2, MSH6, Survivin were 95%, 95%, 10%, respectively. While the positive expression rate of MSH2, MSH6, Survivin were 88.3%, 74.1% and 84.3% in 197 cases of colorectal cancer. Survivin positive expression rate in colorectal cancer group was significantly higher than that in inflammatory control group (P 0.05). MSH2 expression was correlated with tumor size and lymph node metastasis (P 0.05). MSH6 expression was related to gender and lymph node metastasis (P 0.05). Colorectal cancer tissues showed positive correlation between MSH6 and MSH2 (r=0.326, P<0.01), positive correlation between MSH2 and Survivin positive expression (r=0.277, P<0.01), and positive correlation between MSH6 and Survivin positive expression (r=0.435, P<0.01). Conclusions The positive expression rate of Survivin in colorectal cancer is high. MSH2, MSH6 and Survivin in colorectal cancer play an important role in the development and progression of colorectal cancer, and can provide evidence for the detection of these three factors, including metastasis risk, prognosis assessment and clinical treatment. In particular, Survivin gene may provide evidence for gene therapy. Key words: Colorectal neoplasms; MutS homolog 2 protein; MutS homolog 6 protein; Survivin
{"title":"Correlation between serum CTRP3 and CTRP9 levels and glycolipid metabolism in preeclampsia pregnant women","authors":"Lihua Pan, F. Zhou, Y. Ke","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.017","url":null,"abstract":"Objective \u0000To analyze the changes of Survivin, MSH6 and MSH2 expression in colorectal cancer and explore their relationship with clinical and pathological parameters. \u0000 \u0000 \u0000Methods \u0000197 cases of colorectal adenocarcinoma and 20 cases of inflammatory intestinal mucosa were detected by immunohistochemistry with Survivin, MSH6 and MSH2, and the correlation between Survivin, MSH6 and MSH2 expression was analyzed. \u0000 \u0000 \u0000Results \u0000In the control group of 20 cases with inflammatory non-tumor intestinal mucosa, the positive expression rate of MSH2, MSH6, Survivin were 95%, 95%, 10%, respectively. While the positive expression rate of MSH2, MSH6, Survivin were 88.3%, 74.1% and 84.3% in 197 cases of colorectal cancer. Survivin positive expression rate in colorectal cancer group was significantly higher than that in inflammatory control group (P 0.05). MSH2 expression was correlated with tumor size and lymph node metastasis (P 0.05). MSH6 expression was related to gender and lymph node metastasis (P 0.05). Colorectal cancer tissues showed positive correlation between MSH6 and MSH2 (r=0.326, P<0.01), positive correlation between MSH2 and Survivin positive expression (r=0.277, P<0.01), and positive correlation between MSH6 and Survivin positive expression (r=0.435, P<0.01). \u0000 \u0000 \u0000Conclusions \u0000The positive expression rate of Survivin in colorectal cancer is high. MSH2, MSH6 and Survivin in colorectal cancer play an important role in the development and progression of colorectal cancer, and can provide evidence for the detection of these three factors, including metastasis risk, prognosis assessment and clinical treatment. In particular, Survivin gene may provide evidence for gene therapy. \u0000 \u0000 \u0000Key words: \u0000Colorectal neoplasms; MutS homolog 2 protein; MutS homolog 6 protein; Survivin","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90628120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.021
Yiyong Hu, Xian-feng Yang, Jun Lu
Objective To explore the relationship between cerebral hemorrhage and the occurrence of depression after craniocerebral injury surgery. Methods From March 2015 to March 2017, 70 patients with craniocerebral injury in our hospital were selected as the subjects of study. They were divided into the observation group (n=30) and the control group (n=40) according to whether there was cerebral hemorrhage after operation. The National Institutes of Health Stroke Scale score (NIHSS), Hamilton Depression Scale score (HAMD), Glasgow Coma Score (GCS) and the levels of interleukin (IL)-1, IL-6 and high sensitivity C-reactive protein (hs-CRP) in patients with different degrees of cerebral hemorrhage were compared. The correlation between HAMD score and other indexes was analyzed. The influencing factors and independent risk factors of depression were analyzed. Results The levels of NIHSS, HAMD score and serum IL-1, IL-6 and hs-CRP in the observation group were higher than those in the control group after operation (t=13.655, 36.587, 45.274, 46.334, 70.489, P<0.05), while the GCS score was lower than that in the control group (t=15.517, P<0.05). Compared with mild and moderate cerebral hemorrhage, the NIHSS, HAMD scores and serum IL-1, IL-6 and hs-CRP were higher in patients with severe cerebral hemorrhage (F=8.825, 10.254, 23.615, 18.745, 7.245, P<0.05) , while the GCS score was lower (F=7.459, P<0.05). The correlation analysis showed that the postoperative HAMD score was positively correlated with NIHSS score and serum IL-1, IL-6 and hs-CRP (r=0.289, 0.364, 0.333, 0.258, P<0.05) and negatively correlated with the GCS score (r=-0.276, P<0.05). Univariate analysis showed that the level of culture, personality, cerebral hemorrhage degree and serum IL-6 and hs-CRP were the influencing factors of postoperative depression in patients with craniocerebral injury (t or χ2=6.076, 6.300, 8.361, 30.224, 99.575, P<0.05). Multivariate analysis showed that cerebral hemorrhage and hs-CRP were an independent risk factor for postoperative depression (P<0.05). Conclusions Cerebral hemorrhage after craniocerebral injury is closely related to the occurrence of depression, which may be related to the increase of stress-induced inflammatory factors after cerebral hemorrhage. Key words: Craniocerebral trauma; Postoperative hemorrhage; Cerebral hemorrhage; Depressive disorder
{"title":"Relationship between cerebral hemorrhage and depression occurrence after craniocerebral injury surgery","authors":"Yiyong Hu, Xian-feng Yang, Jun Lu","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.021","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.021","url":null,"abstract":"Objective \u0000To explore the relationship between cerebral hemorrhage and the occurrence of depression after craniocerebral injury surgery. \u0000 \u0000 \u0000Methods \u0000From March 2015 to March 2017, 70 patients with craniocerebral injury in our hospital were selected as the subjects of study. They were divided into the observation group (n=30) and the control group (n=40) according to whether there was cerebral hemorrhage after operation. The National Institutes of Health Stroke Scale score (NIHSS), Hamilton Depression Scale score (HAMD), Glasgow Coma Score (GCS) and the levels of interleukin (IL)-1, IL-6 and high sensitivity C-reactive protein (hs-CRP) in patients with different degrees of cerebral hemorrhage were compared. The correlation between HAMD score and other indexes was analyzed. The influencing factors and independent risk factors of depression were analyzed. \u0000 \u0000 \u0000Results \u0000The levels of NIHSS, HAMD score and serum IL-1, IL-6 and hs-CRP in the observation group were higher than those in the control group after operation (t=13.655, 36.587, 45.274, 46.334, 70.489, P<0.05), while the GCS score was lower than that in the control group (t=15.517, P<0.05). Compared with mild and moderate cerebral hemorrhage, the NIHSS, HAMD scores and serum IL-1, IL-6 and hs-CRP were higher in patients with severe cerebral hemorrhage (F=8.825, 10.254, 23.615, 18.745, 7.245, P<0.05) , while the GCS score was lower (F=7.459, P<0.05). The correlation analysis showed that the postoperative HAMD score was positively correlated with NIHSS score and serum IL-1, IL-6 and hs-CRP (r=0.289, 0.364, 0.333, 0.258, P<0.05) and negatively correlated with the GCS score (r=-0.276, P<0.05). Univariate analysis showed that the level of culture, personality, cerebral hemorrhage degree and serum IL-6 and hs-CRP were the influencing factors of postoperative depression in patients with craniocerebral injury (t or χ2=6.076, 6.300, 8.361, 30.224, 99.575, P<0.05). Multivariate analysis showed that cerebral hemorrhage and hs-CRP were an independent risk factor for postoperative depression (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Cerebral hemorrhage after craniocerebral injury is closely related to the occurrence of depression, which may be related to the increase of stress-induced inflammatory factors after cerebral hemorrhage. \u0000 \u0000 \u0000Key words: \u0000Craniocerebral trauma; Postoperative hemorrhage; Cerebral hemorrhage; Depressive disorder","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77136020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.006
Chuqi Yang, Li Zheng, Liane Zhou, Anxia Wan, Shijun Wang
Objective To investigate the relationship between perineural invasion (PNI) and the clinicopathological features of cervical cancer, and to explore its influence on the prognosis of cervical cancer patients. Methods Retrospective analysis the clinical data of 76 patients with early cervical cancer who received radical hysterectomy+ pelvic lymphadenectomy±paraaortic lymphadenectomy from November 2015 to December 2019 in Xuanwu Hospital. The clinicopathological characteristics of the postoperative pathological results of cervical cancer patients were analyzed to explore the related factors of PNI and its influence on prognosis. Results Single factor analysis suggested that the incidence of PNI was related to tumor diameter, depth of stroma invasion, lymph-vascular space invasion, lymph node metastasis and clinical stage (P 0.05). Conclusions PNI is related to the depth of interstitial infiltration. PNI has no significant effect on the short-term recurrence rate and short-term survival rate of cervical cancer patients. Key words: Uterine cervical neoplasms; Perineural invasion; Prognosis; Factor analysis, statistical
{"title":"Analysis of factors related to perineural invasion in cervical cancer and its relationship with prognosis","authors":"Chuqi Yang, Li Zheng, Liane Zhou, Anxia Wan, Shijun Wang","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.006","url":null,"abstract":"Objective \u0000To investigate the relationship between perineural invasion (PNI) and the clinicopathological features of cervical cancer, and to explore its influence on the prognosis of cervical cancer patients. \u0000 \u0000 \u0000Methods \u0000Retrospective analysis the clinical data of 76 patients with early cervical cancer who received radical hysterectomy+ pelvic lymphadenectomy±paraaortic lymphadenectomy from November 2015 to December 2019 in Xuanwu Hospital. The clinicopathological characteristics of the postoperative pathological results of cervical cancer patients were analyzed to explore the related factors of PNI and its influence on prognosis. \u0000 \u0000 \u0000Results \u0000Single factor analysis suggested that the incidence of PNI was related to tumor diameter, depth of stroma invasion, lymph-vascular space invasion, lymph node metastasis and clinical stage (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000PNI is related to the depth of interstitial infiltration. PNI has no significant effect on the short-term recurrence rate and short-term survival rate of cervical cancer patients. \u0000 \u0000 \u0000Key words: \u0000Uterine cervical neoplasms; Perineural invasion; Prognosis; Factor analysis, statistical","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77647925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.012
L. Ren, Yu Wang, S. Pang, L. Fan
Objective To investigate the effect of antisense oligonucleotides of miRNA-34a on non-small cell lung cancer (NSCLC) and its molecular mechanism. Methods The expression of miRNA-34a in human non-small cell lung cancer cell line HCC827 and human normal lung cell MRC-5 was detected by real time fluorescence quantitative polymerase chain reaction (qRT-PCR). HCC827 cells were divided into three groups: blank control group, negative control group, anti-sense oligonucleotide group(liposome 2000 transfected anti-sense oligonucleotide miRNA-34a); cell counting kit-8 (CCK-8) method was used to detect cell proliferation, Jimsa staining was used to detect cell cloning ability, Transwell test was used to detect cell migration and invasion ability; RT-PCR and Western blot were used to detect phosphatase and tensin homolog (PTEN), phosphorylation-protein kinase B (p-Akt), phosphatidylinositol-3-kinase (PI3K) mRNA and protein expression. Results The relative expression of miRNA34a in HCC827 cells was significantly higher than that in human normal lung cells (P 0.05). At 48 h, 72 h and 96 h, the proliferation level of HCC827 cells in anti-sense oligonucleotide miRNA-34a group was significantly lower than that in negative control group and blank control group (P<0.05). The cell cloning rate of antisense oligonucleotide miRNA-34a group was significantly lower than that of negative control group and blank control group (P<0.01). The number of migration and invasion of HCC827 cells in antisense oligonucleotide RNA-34a group was significantly lower than that in negative control group and blank control group (P<0.01). The relative expression of PTEN mRNA and protein in antisense oligonucleotide miRNA-34a group was significantly higher than that in negative control group and blank control group (P<0.05); the relative expression of p-Akt, PI3K mRNA and protein in antisense oligonucleotide miRNA-34a group were significantly lower than that in negative control group and blank control group (P<0.05). Conclusions The expression level of miRNA-34a in human non-small cell lung cancer cells is significantly higher than that in human normal lung cells. Antisense oligonucleotides of miRNA-34a can inhibit the proliferation, cloning, migration and invasion of human non-small cell lung cancer cells. The mechanism may be related to the negative regulation of PTEN/p-Akt/PI3K signaling pathway. Key words: Oligonucleotides, antisense; miRNA-34a; Carcinoma, non-small-cell lung; Cell line, tumor
{"title":"The effect of miRNA-34a antisense oligonucleotide on non-small cell lung cancer cell line HCC827","authors":"L. Ren, Yu Wang, S. Pang, L. Fan","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.012","url":null,"abstract":"Objective \u0000To investigate the effect of antisense oligonucleotides of miRNA-34a on non-small cell lung cancer (NSCLC) and its molecular mechanism. \u0000 \u0000 \u0000Methods \u0000The expression of miRNA-34a in human non-small cell lung cancer cell line HCC827 and human normal lung cell MRC-5 was detected by real time fluorescence quantitative polymerase chain reaction (qRT-PCR). HCC827 cells were divided into three groups: blank control group, negative control group, anti-sense oligonucleotide group(liposome 2000 transfected anti-sense oligonucleotide miRNA-34a); cell counting kit-8 (CCK-8) method was used to detect cell proliferation, Jimsa staining was used to detect cell cloning ability, Transwell test was used to detect cell migration and invasion ability; RT-PCR and Western blot were used to detect phosphatase and tensin homolog (PTEN), phosphorylation-protein kinase B (p-Akt), phosphatidylinositol-3-kinase (PI3K) mRNA and protein expression. \u0000 \u0000 \u0000Results \u0000The relative expression of miRNA34a in HCC827 cells was significantly higher than that in human normal lung cells (P 0.05). At 48 h, 72 h and 96 h, the proliferation level of HCC827 cells in anti-sense oligonucleotide miRNA-34a group was significantly lower than that in negative control group and blank control group (P<0.05). The cell cloning rate of antisense oligonucleotide miRNA-34a group was significantly lower than that of negative control group and blank control group (P<0.01). The number of migration and invasion of HCC827 cells in antisense oligonucleotide RNA-34a group was significantly lower than that in negative control group and blank control group (P<0.01). The relative expression of PTEN mRNA and protein in antisense oligonucleotide miRNA-34a group was significantly higher than that in negative control group and blank control group (P<0.05); the relative expression of p-Akt, PI3K mRNA and protein in antisense oligonucleotide miRNA-34a group were significantly lower than that in negative control group and blank control group (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000The expression level of miRNA-34a in human non-small cell lung cancer cells is significantly higher than that in human normal lung cells. Antisense oligonucleotides of miRNA-34a can inhibit the proliferation, cloning, migration and invasion of human non-small cell lung cancer cells. The mechanism may be related to the negative regulation of PTEN/p-Akt/PI3K signaling pathway. \u0000 \u0000 \u0000Key words: \u0000Oligonucleotides, antisense; miRNA-34a; Carcinoma, non-small-cell lung; Cell line, tumor","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80190341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.010
Yue Li, Ning Li, Xiuqin An, Jinchun Liu
Objective To investigate the effects of myeloid-derived suppressor cells (MDSCs) on nonalcoholic steatohepatitis mice. Methods ⑴ Male C57BL/6 mice aged 6-8 weeks were randomly divided into normal diet group, CDAA group(choline deficient amino acid diet) and CSAA group (choline sufficient amino acid diet). ⑵ After the success of the non-alcoholic steatohepatitis model, serum was collected from some mice to detect biochemical indexes; Liver tissue was retained for microscopic observation by hematoxylin-eosin (HE) staining; The changes of T cell subsets in peripheral blood of mice in each group were detected by flow cytometry. In addition, The proportion and subtype of CD11b+ Gr-1+ MDSCs cells in liver, spleen, blood and bone marrow were also detected by flow cytometry. ⑶ The bone marrow-derived Gr-1highLy-6G+ was purified by magnetic bead sorting technique, and the purified Gr-1highLy-6G+ was transferred into non-alcoholic steatohepatitis (NASH) mice by tail vein injection. The role of MDSCs in NASH was analyzed by detection of serological indexes of liver function and pathological dyeing. Results ⑴ There was no significant difference in body weight and liver index between the groups (P>0.05). Serological indicators: alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood glucose, interleukin (IL)-6 and interferon-γ (INF-γ) in the model group were significantly higher than those in the normal group (P 0.05); the MDSCs of liver in CDAA group is lower than that of normal group (P normal group (P<0.01), CD11b+ Gr-1dimLy-6G-(M-MDSC) showed a downward trend, CDAA group < normal group; ⑶ Serum AST and ALT levels of NASH mice who receiving Gr-1highLy-6G+ MDSCs from normal bone marrow were significantly decreased (P<0.001), and histopathological changes were alleviated. Conclusions ⑴ The NASA mouse model can be successfully established on the CDAA diet. ⑵ The CDAA-induced NASH mice may have immune dysfunction, mainly manifesting in the change of bone marrow MDSCs subpopulations and the increase of CD11b+ Gr-1highLy-6G+ (G-MDSC). ⑶ MDSCs derived from normal mouse bone marrow can alleviate the pathological changes of NASH. Key words: Myeloid-derived suppressor cells; Non-alcoholic steatohepatitis; T-lymphocytes; Interleukin-6; Mice
{"title":"The role and mechanism of myeloid-derived suppressor cells in mice with nonalcoholic steatohepatitis","authors":"Yue Li, Ning Li, Xiuqin An, Jinchun Liu","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.010","url":null,"abstract":"Objective \u0000To investigate the effects of myeloid-derived suppressor cells (MDSCs) on nonalcoholic steatohepatitis mice. \u0000 \u0000 \u0000Methods \u0000⑴ Male C57BL/6 mice aged 6-8 weeks were randomly divided into normal diet group, CDAA group(choline deficient amino acid diet) and CSAA group (choline sufficient amino acid diet). ⑵ After the success of the non-alcoholic steatohepatitis model, serum was collected from some mice to detect biochemical indexes; Liver tissue was retained for microscopic observation by hematoxylin-eosin (HE) staining; The changes of T cell subsets in peripheral blood of mice in each group were detected by flow cytometry. In addition, The proportion and subtype of CD11b+ Gr-1+ MDSCs cells in liver, spleen, blood and bone marrow were also detected by flow cytometry. ⑶ The bone marrow-derived Gr-1highLy-6G+ was purified by magnetic bead sorting technique, and the purified Gr-1highLy-6G+ was transferred into non-alcoholic steatohepatitis (NASH) mice by tail vein injection. The role of MDSCs in NASH was analyzed by detection of serological indexes of liver function and pathological dyeing. \u0000 \u0000 \u0000Results \u0000⑴ There was no significant difference in body weight and liver index between the groups (P>0.05). Serological indicators: alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood glucose, interleukin (IL)-6 and interferon-γ (INF-γ) in the model group were significantly higher than those in the normal group (P 0.05); the MDSCs of liver in CDAA group is lower than that of normal group (P normal group (P<0.01), CD11b+ Gr-1dimLy-6G-(M-MDSC) showed a downward trend, CDAA group < normal group; ⑶ Serum AST and ALT levels of NASH mice who receiving Gr-1highLy-6G+ MDSCs from normal bone marrow were significantly decreased (P<0.001), and histopathological changes were alleviated. \u0000 \u0000 \u0000Conclusions \u0000⑴ The NASA mouse model can be successfully established on the CDAA diet. ⑵ The CDAA-induced NASH mice may have immune dysfunction, mainly manifesting in the change of bone marrow MDSCs subpopulations and the increase of CD11b+ Gr-1highLy-6G+ (G-MDSC). ⑶ MDSCs derived from normal mouse bone marrow can alleviate the pathological changes of NASH. \u0000 \u0000 \u0000Key words: \u0000Myeloid-derived suppressor cells; Non-alcoholic steatohepatitis; T-lymphocytes; Interleukin-6; Mice","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81493990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.019
Shengwei Zou, Huaizhong Huang, Jun Liu, Jian-hua Xiong
Objective To compare the influences of microsurgical clipping and intravascular interventional embolization on the nerve function recovery in patients with posterior communicating aneurysm combined with oculomotor paralyses. Methods Eighty-six patients with posterior communicating artery aneurysm accompanied by oculomotor nerve palsy admitted to Qianjiang Central Hospital of Chongqing from May 2015 to May 2017 were randomly divided into observation group (n=43) and control group (n=43) by random number table method. The control group was treated with microsurgical clipping, while the observation group was treated with intravascular interventional embolization. The therapeutic effect, neurological function recovery, postoperative complications and prognosis of the two groups were compared. Results The operation time, intraoperative blood loss, postoperative respiratory recovery time and postoperative extubation time of the observation group were significantly less than those of the control group (P<0.05); the complete recovery of the observation group was significantly higher than that of the control group, and no recovery was significantly lower than that of the control group (P<0.05); the incidence of complications in the observation group was significantly lower than that in the control group (P<0.05); the proportion of 5 points in the observation group was significantly higher than that in the control group (P<0.05). Conclusions Intravascular interventional embolization in the treatment of posterior communicating aneurysm combined with oculomotor paralyses can promote the recovery of nerve function, reduce the incidence of postoperative complications, have a good prognosis and a definite effect, which can be used as the first choice of treatment. Key words: Microsurgery; Microsurgical clipping; Intravascular interventional emboliization; Posterior communicating aneurysm; Oculomotor paralyses; Nerve function
{"title":"Comparison of microsurgical clipping and intravascular interventional embolization on nerve function recovery in patients with posterior communicating aneurysm combined with oculomotor paralyses","authors":"Shengwei Zou, Huaizhong Huang, Jun Liu, Jian-hua Xiong","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.019","url":null,"abstract":"Objective \u0000To compare the influences of microsurgical clipping and intravascular interventional embolization on the nerve function recovery in patients with posterior communicating aneurysm combined with oculomotor paralyses. \u0000 \u0000 \u0000Methods \u0000Eighty-six patients with posterior communicating artery aneurysm accompanied by oculomotor nerve palsy admitted to Qianjiang Central Hospital of Chongqing from May 2015 to May 2017 were randomly divided into observation group (n=43) and control group (n=43) by random number table method. The control group was treated with microsurgical clipping, while the observation group was treated with intravascular interventional embolization. The therapeutic effect, neurological function recovery, postoperative complications and prognosis of the two groups were compared. \u0000 \u0000 \u0000Results \u0000The operation time, intraoperative blood loss, postoperative respiratory recovery time and postoperative extubation time of the observation group were significantly less than those of the control group (P<0.05); the complete recovery of the observation group was significantly higher than that of the control group, and no recovery was significantly lower than that of the control group (P<0.05); the incidence of complications in the observation group was significantly lower than that in the control group (P<0.05); the proportion of 5 points in the observation group was significantly higher than that in the control group (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Intravascular interventional embolization in the treatment of posterior communicating aneurysm combined with oculomotor paralyses can promote the recovery of nerve function, reduce the incidence of postoperative complications, have a good prognosis and a definite effect, which can be used as the first choice of treatment. \u0000 \u0000 \u0000Key words: \u0000Microsurgery; Microsurgical clipping; Intravascular interventional emboliization; Posterior communicating aneurysm; Oculomotor paralyses; Nerve function","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73397888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.004
R. Bai, Xiaohan Xu, Kaili Li, Ping-shan Yang
Objective To investigate the causes, precautions, and treatment of ureteral injury associated with laparoscopic radical surgery for locally advanced cervical cancer. Methods The clinical data of patients with locally advanced cervical cancer suffering from ureteral injury occurred during or after laparoscopic radical hysterectomy and pelvic lymphadenectomy performed at our hospital between January 2014 and December 2018 were retrospectively analyzed. Results Totally, fifty-four patients with locally advanced cervical cancer underwent laparoscopic radical surgery. Intraoperative ureteral injury occurred in one woman, for whom laparoscopic ureteral end-to-end anastomosis and stent implantation were performed immediately. Postoperative hydroureter was observed in eight patients and treated by ureteral stent implantation under ureteroscopy. There were four cases of postoperative ureteral fistula: one patient with bilateral ureteral fistula was, after failing in placing bilateral ureteral stents under ureteroscopy, subjected to laparotomy for stent implantation into the left ureter and end-to-end anastomosis of the right ureter; one patient with right ureteral fistula underwent ureteroscopic ureteral stenting; one patient with left ureteral fistula received a laparotomic ureteroplasty with bladder wall flap; another patient with right ureteral fistula who had symptoms of leaking urine 14 days after surgery, but sought treatment until one month after surgery, underwent percutaneous nephrostomy after a failed ureteral intubation, followed by a ureterovesical replantation after six months. All patients who had received ureteral catheterization underwent extraction of ureteral stents after three to six months and recovered well. Conclusions The radical surgery for locally advanced cervical cancer requires an extensive surgical procedure because of the bulky size of the lesion, which is associated with an increased risk of ureteral injury. With early detection and proper treatment, a good prognosis of the ureteral injury can be expected. Key words: Uterine cervical neoplasms; Laparoscopy; Operative complications; Ureteral injury
{"title":"Clinical analysis and treatment of ureteral injury caused by laparoscopic surgery in patients with locally advanced cervical cancer","authors":"R. Bai, Xiaohan Xu, Kaili Li, Ping-shan Yang","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.004","url":null,"abstract":"Objective \u0000To investigate the causes, precautions, and treatment of ureteral injury associated with laparoscopic radical surgery for locally advanced cervical cancer. \u0000 \u0000 \u0000Methods \u0000The clinical data of patients with locally advanced cervical cancer suffering from ureteral injury occurred during or after laparoscopic radical hysterectomy and pelvic lymphadenectomy performed at our hospital between January 2014 and December 2018 were retrospectively analyzed. \u0000 \u0000 \u0000Results \u0000Totally, fifty-four patients with locally advanced cervical cancer underwent laparoscopic radical surgery. Intraoperative ureteral injury occurred in one woman, for whom laparoscopic ureteral end-to-end anastomosis and stent implantation were performed immediately. Postoperative hydroureter was observed in eight patients and treated by ureteral stent implantation under ureteroscopy. There were four cases of postoperative ureteral fistula: one patient with bilateral ureteral fistula was, after failing in placing bilateral ureteral stents under ureteroscopy, subjected to laparotomy for stent implantation into the left ureter and end-to-end anastomosis of the right ureter; one patient with right ureteral fistula underwent ureteroscopic ureteral stenting; one patient with left ureteral fistula received a laparotomic ureteroplasty with bladder wall flap; another patient with right ureteral fistula who had symptoms of leaking urine 14 days after surgery, but sought treatment until one month after surgery, underwent percutaneous nephrostomy after a failed ureteral intubation, followed by a ureterovesical replantation after six months. All patients who had received ureteral catheterization underwent extraction of ureteral stents after three to six months and recovered well. \u0000 \u0000 \u0000Conclusions \u0000The radical surgery for locally advanced cervical cancer requires an extensive surgical procedure because of the bulky size of the lesion, which is associated with an increased risk of ureteral injury. With early detection and proper treatment, a good prognosis of the ureteral injury can be expected. \u0000 \u0000 \u0000Key words: \u0000Uterine cervical neoplasms; Laparoscopy; Operative complications; Ureteral injury","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80423566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.020
Wan Zhou, Heng Zhang, Geping Zhang, Cuirong Bai
Objective To investigate the diagnostic values of ultrasonography combined with serum cancer antigen 125 (CA125), serum carbohydrate antigen 199 (CA199), serum carcinoembryonic antigen (CEA) in ovarian malignant tumors. Methods From November 2015 to December 2017, 140 patients with ovarian cancer admitted to the hospital were selected as subjects, and ultrasound examination was carried out with IU22 ultrasound diagnostic instrument. The patients were divided into 92 cases of benign ovarian tumors and 48 cases of malignant ovarian tumors through pathological diagnosis. The serum CA125, CA199 and CEA of all patients were detected by automatic biochemical analyzer, and receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of four combined detection in ovarian malignancies. Results The coincidence rate of ultrasound diagnosis in ovarian benign tumor patients was significantly higher than that in ovarian cancer patients (P<0.05). The levels of serum CA125, CA199 and CEA in benign ovarian tumors were significantly lower than those in malignant ovarian tumors (P<0.05), and the levels of serum CA125, CA199 and CEA in patients with stage Ⅰ to Ⅱ were significantly lower than those in stage Ⅲ to Ⅳ (P<0.05). The area of serum CA125, CA199 and CEA under the ROC curve was 0.788, 0.750 and 0.708, respectively. The boundary points of diagnosis were 50.61 U/ml, 36.47 U/ml and 4.32 ng/ml, respectively. The area under the combined diagnosis curve of the serum CA125, CA199 and CEA was 0.832. The sensitivity, specificity and accuracy of the combined detection were higher than that of single index detection and serum combined detection. Conclusions Ultrasound combined with serum CA125, CA199 and CEA levels detection can improve the sensitivity, specificity and accuracy of diagnosis of ovarian benign and malignant tumors, which has certain clinical value. Key words: Ultrasonography; CA125; CA199; Carcinoembryonic antigen; Ovarian neoplasms
{"title":"Value of ultrasound combined with serum CA125, CA199 and CEA in the differential diagnosis of ovarian benign and malignant tumors","authors":"Wan Zhou, Heng Zhang, Geping Zhang, Cuirong Bai","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.020","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.020","url":null,"abstract":"Objective \u0000To investigate the diagnostic values of ultrasonography combined with serum cancer antigen 125 (CA125), serum carbohydrate antigen 199 (CA199), serum carcinoembryonic antigen (CEA) in ovarian malignant tumors. \u0000 \u0000 \u0000Methods \u0000From November 2015 to December 2017, 140 patients with ovarian cancer admitted to the hospital were selected as subjects, and ultrasound examination was carried out with IU22 ultrasound diagnostic instrument. The patients were divided into 92 cases of benign ovarian tumors and 48 cases of malignant ovarian tumors through pathological diagnosis. The serum CA125, CA199 and CEA of all patients were detected by automatic biochemical analyzer, and receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of four combined detection in ovarian malignancies. \u0000 \u0000 \u0000Results \u0000The coincidence rate of ultrasound diagnosis in ovarian benign tumor patients was significantly higher than that in ovarian cancer patients (P<0.05). The levels of serum CA125, CA199 and CEA in benign ovarian tumors were significantly lower than those in malignant ovarian tumors (P<0.05), and the levels of serum CA125, CA199 and CEA in patients with stage Ⅰ to Ⅱ were significantly lower than those in stage Ⅲ to Ⅳ (P<0.05). The area of serum CA125, CA199 and CEA under the ROC curve was 0.788, 0.750 and 0.708, respectively. The boundary points of diagnosis were 50.61 U/ml, 36.47 U/ml and 4.32 ng/ml, respectively. The area under the combined diagnosis curve of the serum CA125, CA199 and CEA was 0.832. The sensitivity, specificity and accuracy of the combined detection were higher than that of single index detection and serum combined detection. \u0000 \u0000 \u0000Conclusions \u0000Ultrasound combined with serum CA125, CA199 and CEA levels detection can improve the sensitivity, specificity and accuracy of diagnosis of ovarian benign and malignant tumors, which has certain clinical value. \u0000 \u0000 \u0000Key words: \u0000Ultrasonography; CA125; CA199; Carcinoembryonic antigen; Ovarian neoplasms","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75266452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-29DOI: 10.3760/CMA.J.ISSN.1008-1372.2020.02.014
Xueyao Gu, Jun Wang, Yanxin Xiao, Xincui Zhao, Yaling Han
Objective To observe the expression of FGF19 ( fibroblast growth factor 19) and PEDF (pigment epithelium-derived factor) in the serum of type 2 diabetic retinopathy (DR) patients and discuss their significance. Methods Total 89 patients with type 2 diabetes were selected and divided into two groups according to whether they were combined with diabetic retinopathy: 45 patients with type 2 diabetes alone (DM group) and 44 patients with type 2 diabetes mellitus combined with retinopathy (DR group). At the same time, 40 healthy people were selected as the control group (NDM group). The serum levels of FGF19 and PEDF and their biochemical indexes were detected and compared in each group. The correlation between the indexes and the relationship between FGF19, PEDF and DR were analyzed. Results Compared with NDM group, serum FGF19 level in DM group and DR group decreased, while C-reactive protein (CRP) and PEDF level in DM group and DR group increased (P 0.05); the levels of Hcy, triacylglycerol (TG) and CRP in DR group were higher than those in DM and NDM group (P 12.76 mg/L, the sensitivity and specificity of PEDF dignosing DR were 80.9% and 56.7% respectively. Conclusions In patients with DR, serum FGF19 level decreased and serum PEDF levels increased. The level of both changes is closely related to DR and may be involved in the development of DR. Key words: Diabetic retinopathy; Diabetes mellitus, type 2; Fibroblast growth factor 19; Pigment epithelium derived factor
{"title":"Changes of serum FGF19 and PEDF levels in patients with type 2 diabetic retinopathy","authors":"Xueyao Gu, Jun Wang, Yanxin Xiao, Xincui Zhao, Yaling Han","doi":"10.3760/CMA.J.ISSN.1008-1372.2020.02.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2020.02.014","url":null,"abstract":"Objective \u0000To observe the expression of FGF19 ( fibroblast growth factor 19) and PEDF (pigment epithelium-derived factor) in the serum of type 2 diabetic retinopathy (DR) patients and discuss their significance. \u0000 \u0000 \u0000Methods \u0000Total 89 patients with type 2 diabetes were selected and divided into two groups according to whether they were combined with diabetic retinopathy: 45 patients with type 2 diabetes alone (DM group) and 44 patients with type 2 diabetes mellitus combined with retinopathy (DR group). At the same time, 40 healthy people were selected as the control group (NDM group). The serum levels of FGF19 and PEDF and their biochemical indexes were detected and compared in each group. The correlation between the indexes and the relationship between FGF19, PEDF and DR were analyzed. \u0000 \u0000 \u0000Results \u0000Compared with NDM group, serum FGF19 level in DM group and DR group decreased, while C-reactive protein (CRP) and PEDF level in DM group and DR group increased (P 0.05); the levels of Hcy, triacylglycerol (TG) and CRP in DR group were higher than those in DM and NDM group (P 12.76 mg/L, the sensitivity and specificity of PEDF dignosing DR were 80.9% and 56.7% respectively. \u0000 \u0000 \u0000Conclusions \u0000In patients with DR, serum FGF19 level decreased and serum PEDF levels increased. The level of both changes is closely related to DR and may be involved in the development of DR. \u0000 \u0000 \u0000Key words: \u0000Diabetic retinopathy; Diabetes mellitus, type 2; Fibroblast growth factor 19; Pigment epithelium derived factor","PeriodicalId":15276,"journal":{"name":"中国医师杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73035986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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