The existing treatments have no satisfactory outcomes for hepatocellular carcinoma (HCC). LMO1 is abnormally expressed in several tumors, but its relationship with HCC is unclear. Therefore, we intend to evaluate LMO1’s role in HCC and the clinical significance. The HCC patients cancer tissues and adjacent tissues were collected. LMO1/control-siRNA was transfected into HepG2 cells followed by analysis of relevant protein expression by immunohistochemistry and Western blot, LMO1 mRNA level by qPCR, cell proliferation by CCK8 assay and apoptosis by flow cytometry. LMO1 positive rate was significantly elevated in HCC tissues relative to adjacent tissues. LMO1 expression was related to TNM stage and lymph node metastasis of tumor tissues (P <0.05). Patients with positive LMO1 level had a lower cumulative survival rate than those with negative expression (P <0.05). TNM stage, lymphatic metastasis and LMO1 expression were identified to be independent risk factors affecting HCC survival; cells transfected with LMO1-siRNA showed significantly reduced proliferation, increased cell apoptosis and upregulated bax as well as downregulated bcl-2 (P < 0.05). In conclusion, LMO1 may affect the occurrence and development of HCC through regulation of the bcl-2/bax expression.
{"title":"LMO1 Promotes the Development of Hepatocellular Carcinoma by Regulation of bcl-2 and bax","authors":"Jianfeng Zhang, B. Peng, Zhuowen Li, Yuan Zhang, Yongjun Zhang, Xiushan Lu, Yanwen Cao, Zijun Dai, Chuanyin Xiong","doi":"10.1166/jbt.2023.3277","DOIUrl":"https://doi.org/10.1166/jbt.2023.3277","url":null,"abstract":"The existing treatments have no satisfactory outcomes for hepatocellular carcinoma (HCC). LMO1 is abnormally expressed in several tumors, but its relationship with HCC is unclear. Therefore, we intend to evaluate LMO1’s role in HCC and the clinical significance. The HCC patients\u0000 cancer tissues and adjacent tissues were collected. LMO1/control-siRNA was transfected into HepG2 cells followed by analysis of relevant protein expression by immunohistochemistry and Western blot, LMO1 mRNA level by qPCR, cell proliferation by CCK8 assay and apoptosis by flow cytometry. LMO1\u0000 positive rate was significantly elevated in HCC tissues relative to adjacent tissues. LMO1 expression was related to TNM stage and lymph node metastasis of tumor tissues (P <0.05). Patients with positive LMO1 level had a lower cumulative survival rate than those with negative expression\u0000 (P <0.05). TNM stage, lymphatic metastasis and LMO1 expression were identified to be independent risk factors affecting HCC survival; cells transfected with LMO1-siRNA showed significantly reduced proliferation, increased cell apoptosis and upregulated bax as well as downregulated\u0000 bcl-2 (P < 0.05). In conclusion, LMO1 may affect the occurrence and development of HCC through regulation of the bcl-2/bax expression.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42119817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study intends to assess the biological effect of TTR on human retinal endothelial cells under high glucose. Cells were assigned into normal group, high glucose (HG) group and TTR intervention group followed by analysis of cell biological activity and protein expression. The retinal endothelial cell proliferation rate was significantly promoted in HG group (P < 0.05) and inhibited in intervention group (P < 0.05). The HG group had significantly higher cell migration number than normal group (P < 0.05). However, migrated cell number in intervention group was reduced significantly (P < 0.05). In addition, high glucose also significantly enhanced the invasion of retinal endothelial cells (P < 0.05), which was inhibited after TTR intervention (P < 0.05). Moreover, Bcl-2 protein was significantly downregulated and Bax was upregulated in HG group compared to normal group (P < 0.05). Interestingly, their levels were normalized after TTR intervention without difference compared to their levels in normal group (P < 0.05). Consistently, the mRNA level of Bcl-2 and Bax showed similar expression profiles to the protein expression in different groups (P < 0.05). In conclusion, TTR can inhibit the retinal endothelial cell proliferation, migration and invasion ability and regulate Bcl-2/Bax expression.
{"title":"The Effect of Thyroxin on the Biological Effects of High Glucose-Induced Human Retinal Endothelial Cells and B-Cell Lymphoma 2/Bcl-2 Associated X","authors":"Lei Liu, Xiaoyong Yuan, Yanlin Gao","doi":"10.1166/jbt.2023.3250","DOIUrl":"https://doi.org/10.1166/jbt.2023.3250","url":null,"abstract":"This study intends to assess the biological effect of TTR on human retinal endothelial cells under high glucose. Cells were assigned into normal group, high glucose (HG) group and TTR intervention group followed by analysis of cell biological activity and protein expression. The retinal\u0000 endothelial cell proliferation rate was significantly promoted in HG group (P < 0.05) and inhibited in intervention group (P < 0.05). The HG group had significantly higher cell migration number than normal group (P < 0.05). However, migrated cell number in intervention\u0000 group was reduced significantly (P < 0.05). In addition, high glucose also significantly enhanced the invasion of retinal endothelial cells (P < 0.05), which was inhibited after TTR intervention (P < 0.05). Moreover, Bcl-2 protein was significantly downregulated\u0000 and Bax was upregulated in HG group compared to normal group (P < 0.05). Interestingly, their levels were normalized after TTR intervention without difference compared to their levels in normal group (P < 0.05). Consistently, the mRNA level of Bcl-2 and Bax showed similar\u0000 expression profiles to the protein expression in different groups (P < 0.05). In conclusion, TTR can inhibit the retinal endothelial cell proliferation, migration and invasion ability and regulate Bcl-2/Bax expression.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46984796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
miR-10a participates in the prognosis of patients with sepsis, which also influence multiple organs and cause damages to the kidney and myocardial tissues. This study intends to assess miR-10a’s role in sepsis-induced renal and myocardial injury. 50 Wistar rats were randomized into sham-operation group, model group, MiR-10a group, positive control group and PI3K/AKT-agonist group (n = 10) followed by analysis of the histopathological changes of myocardial and renal tissues, kidney injury, expression of renal GR-α and CK-MB/CK, levels of inflammatory factors (IL-10, IL-6, IL-1β and TNF-α) and the level of miR-10a, PI3K and AKT. Rats in model group and PI3K/AKT-agonist group exhibited highest pathological score of kidney injury, expression of CK-MB, CK and renal GR-α, followed by rats in positive control group and miR-10a group. Furthermore, model group and PI3K/AKT-agonist group showed the highest level of inflammatory factors (TNF-α, IL-1β, IL-6, and IL-10), followed by positive control group and miR-10a group. Lowest miR-10a expression and highest mRNA levels of PI3K and AKT was detected in model group, PI3K/AKT-agonist group and positive control group, followed by miR-10a group. PI3K is a target of miR-10a. In conclusion, miR-10a alleviates the sepsis-induced renal and myocardial injury mainly by mediating the PI3K/AKT transduction pathway, indicating that miR-10a can be utilized as a target gene for sepsis treatment.
{"title":"miR-10a Ameliorates Renal and Myocardial Injury in Sepsis Through Regulation of PI3K/AKT Signaling","authors":"Chenglian Hu, Ying Yang, Lunhun Ye","doi":"10.1166/jbt.2023.3255","DOIUrl":"https://doi.org/10.1166/jbt.2023.3255","url":null,"abstract":"miR-10a participates in the prognosis of patients with sepsis, which also influence multiple organs and cause damages to the kidney and myocardial tissues. This study intends to assess miR-10a’s role in sepsis-induced renal and myocardial injury. 50 Wistar rats were randomized\u0000 into sham-operation group, model group, MiR-10a group, positive control group and PI3K/AKT-agonist group (n = 10) followed by analysis of the histopathological changes of myocardial and renal tissues, kidney injury, expression of renal GR-α and CK-MB/CK, levels of inflammatory\u0000 factors (IL-10, IL-6, IL-1β and TNF-α) and the level of miR-10a, PI3K and AKT. Rats in model group and PI3K/AKT-agonist group exhibited highest pathological score of kidney injury, expression of CK-MB, CK and renal GR-α, followed by rats in positive control\u0000 group and miR-10a group. Furthermore, model group and PI3K/AKT-agonist group showed the highest level of inflammatory factors (TNF-α, IL-1β, IL-6, and IL-10), followed by positive control group and miR-10a group. Lowest miR-10a expression and highest mRNA levels of\u0000 PI3K and AKT was detected in model group, PI3K/AKT-agonist group and positive control group, followed by miR-10a group. PI3K is a target of miR-10a. In conclusion, miR-10a alleviates the sepsis-induced renal and myocardial injury mainly by mediating the PI3K/AKT transduction pathway, indicating\u0000 that miR-10a can be utilized as a target gene for sepsis treatment.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42551025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hong Zhang, Liang Tao, Xinrui Zhang, Hongyan Zhang
This study assesses the role of CHRDL1 (Chordin-like 1) i in the bone marrow stromal cells (BMSC) proliferation and excretion of exosome and myocardial damage under high sugar. BMSC from rats with high CHRDL1 expression was established. The exosome in the supernatant of BMSC with high CHRDL1 level was collected. H9C2 cells were assigned into control set, high sugar set and exo-CHRDL1-BMSC set followed by analysis of CHRDL1 level, BMSC proliferation and apoptosis, expression of Beclin-1, Atg5, Bcl-2 and Bax, and ROS and SOD activity. Cell proliferation was prompted and apoptotic activity was reduced in exo-CHRDL1-BMSC set with reduced ROS activity and increased SOD activity as well as upregulated Bcl-2 and downregulated Bax. In addition, exo-CHRDL1-BMSC set presented increased CHRDL1 secretion and upregulated Beclin-1 and Atg5 expression. In conclusion, proliferation of BMSC under high sugar is prompted and apoptosis is reduced by CHRDL1 through regulating the autophagy.
{"title":"Chordin-Like 1 Regulates Bone Marrow Stem Cell Proliferation and Excretion of Exosome and Myocardial Damage Under High Sugar","authors":"Hong Zhang, Liang Tao, Xinrui Zhang, Hongyan Zhang","doi":"10.1166/jbt.2023.3248","DOIUrl":"https://doi.org/10.1166/jbt.2023.3248","url":null,"abstract":"This study assesses the role of CHRDL1 (Chordin-like 1) i in the bone marrow stromal cells (BMSC) proliferation and excretion of exosome and myocardial damage under high sugar. BMSC from rats with high CHRDL1 expression was established. The exosome in the supernatant of BMSC with high\u0000 CHRDL1 level was collected. H9C2 cells were assigned into control set, high sugar set and exo-CHRDL1-BMSC set followed by analysis of CHRDL1 level, BMSC proliferation and apoptosis, expression of Beclin-1, Atg5, Bcl-2 and Bax, and ROS and SOD activity. Cell proliferation was prompted and apoptotic\u0000 activity was reduced in exo-CHRDL1-BMSC set with reduced ROS activity and increased SOD activity as well as upregulated Bcl-2 and downregulated Bax. In addition, exo-CHRDL1-BMSC set presented increased CHRDL1 secretion and upregulated Beclin-1 and Atg5 expression. In conclusion, proliferation\u0000 of BMSC under high sugar is prompted and apoptosis is reduced by CHRDL1 through regulating the autophagy.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46855744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To investigate the effect of bone marrow mesenchymal stem cells (bmsc) on RA-fibroblast-like synovial cells (FLS) and collagen-induced arthritis (CIA), and to further explore the mechanism of action. Methods: The rheumatoid arthritis cell model was established, and the cells were co-cultured with BMSC. The proliferation and apoptosis of the cells were detected by MTT and flow cytometry, the expression level of inflammatory factors in the cells was detected by ELISA, and the phosphorylation activation and expression of proteins in related pathways were analyzed by western blotting. Results: bmsc inhibited the proliferation of TNF-a-induced RA-FLS, increased the apoptosis rate, and up-regulated caspase-3, PARP and Burlington levels. Meanwhile, the expression of il-10, il-1β and il-6 was inhibited. p-STAT3 levels were down-regulated in a dose-dependent manner. Overexpression of STAT3 partially neutralizes BMSC-mediated caspase-3 increase and PARP shear, as well as down-regulation of il-10, IL-1B, and il-6. This suggests that BMSCs inactivate the STAT3 pathway. In addition, BMSCs can effectively inhibit the production of inflammatory cytokines in rat models of RA-FLS and CIA. Conclusions: In summary, synthesis is a potential long-term treatment drug for rheumatoid arthritis, which can play a therapeutic role in rheumatoid arthritis by inactivating the STAT3 pathway. At the same time, it reveals the role of STAT3 pathway in the pathogenesis of rheumatoid arthritis, and suggests the possibility of STAT3 pathway as a therapeutic target for rheumatoid arthritis.
{"title":"Co-Culture of Bone Marrow Mesenchymal Stem Cells and Fibroblast-Like Synoviocytes (RA-FLS) Alleviates Rheumatoid Arthritis Cell Apoptosis by Inhibiting Inflammatory Response","authors":"qingchen liang, Yanjie Tian, Zijin Liu, Dejun Yu, Hengbing Guo, Fenglong Sun","doi":"10.1166/jbt.2023.3254","DOIUrl":"https://doi.org/10.1166/jbt.2023.3254","url":null,"abstract":"Objective: To investigate the effect of bone marrow mesenchymal stem cells (bmsc) on RA-fibroblast-like synovial cells (FLS) and collagen-induced arthritis (CIA), and to further explore the mechanism of action. Methods: The rheumatoid arthritis cell model was established,\u0000 and the cells were co-cultured with BMSC. The proliferation and apoptosis of the cells were detected by MTT and flow cytometry, the expression level of inflammatory factors in the cells was detected by ELISA, and the phosphorylation activation and expression of proteins in related pathways\u0000 were analyzed by western blotting. Results: bmsc inhibited the proliferation of TNF-a-induced RA-FLS, increased the apoptosis rate, and up-regulated caspase-3, PARP and Burlington levels. Meanwhile, the expression of il-10, il-1β and il-6 was inhibited. p-STAT3 levels were\u0000 down-regulated in a dose-dependent manner. Overexpression of STAT3 partially neutralizes BMSC-mediated caspase-3 increase and PARP shear, as well as down-regulation of il-10, IL-1B, and il-6. This suggests that BMSCs inactivate the STAT3 pathway. In addition, BMSCs can effectively inhibit\u0000 the production of inflammatory cytokines in rat models of RA-FLS and CIA. Conclusions: In summary, synthesis is a potential long-term treatment drug for rheumatoid arthritis, which can play a therapeutic role in rheumatoid arthritis by inactivating the STAT3 pathway. At the same time,\u0000 it reveals the role of STAT3 pathway in the pathogenesis of rheumatoid arthritis, and suggests the possibility of STAT3 pathway as a therapeutic target for rheumatoid arthritis.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45510191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMSCs can effectively reduce the accumulation of extracellular matrix in renal tissues of rats with FSGS. This study intends to investigate the effect of BMSCs on extracellular matrix accumulation in renal tissues of rats with FSGS. 60 female mice aged 3 weeks were randomly assigned into control group, model group, transplantation group, positive control group and p38MAPK signaling pathway agonist group (agonist group) followed by analysis of renal histopathology, body weight, kidney weight and renal weight index of rats, expression of Col-IV, FN, MMP-9 protein and mRNA, as well as P38MAPK, P-CREB protein expression. The body weight, kidney weight and kidney weight index of rats in control group, transplant group and positive control group were significantly lower than those in model group and agonist group (P < 0.05) without differences among control group, transplantation group, positive control group, model group and agonist group (P > 0.05). COL-IV and FN protein and mRNA expression was higher in control group, transplantation group and positive control group, while MMP-9 expression was lower. However, agonist group and model group showed opposite profile of these proteins (P < 0.05). The protein expressions of p38MAPK and P-CREB in control group and transplantation group were lower than those in model group, positive control group and agonist group (P < 0.05) without significant difference among other groups (P > 0.05). In conclusion, BMSCs can reduce the accumulation of extracellular matrix and glomerular sclerosis, thereby controlling FSGS progress.
{"title":"Role of Bone Marrow Mesenchymal Stem Cells in p38MAPK Signaling Pathway and Extracellular Matrix Accumulation in Renal Tissue of Focal Segmental Glomerulosclerosis","authors":"Han Min, Xiumin Gong, Xiaomeng Cai","doi":"10.1166/jbt.2023.3252","DOIUrl":"https://doi.org/10.1166/jbt.2023.3252","url":null,"abstract":"BMSCs can effectively reduce the accumulation of extracellular matrix in renal tissues of rats with FSGS. This study intends to investigate the effect of BMSCs on extracellular matrix accumulation in renal tissues of rats with FSGS. 60 female mice aged 3 weeks were randomly assigned\u0000 into control group, model group, transplantation group, positive control group and p38MAPK signaling pathway agonist group (agonist group) followed by analysis of renal histopathology, body weight, kidney weight and renal weight index of rats, expression of Col-IV, FN, MMP-9 protein and mRNA,\u0000 as well as P38MAPK, P-CREB protein expression. The body weight, kidney weight and kidney weight index of rats in control group, transplant group and positive control group were significantly lower than those in model group and agonist group (P < 0.05) without differences among control\u0000 group, transplantation group, positive control group, model group and agonist group (P > 0.05). COL-IV and FN protein and mRNA expression was higher in control group, transplantation group and positive control group, while MMP-9 expression was lower. However, agonist group and model\u0000 group showed opposite profile of these proteins (P < 0.05). The protein expressions of p38MAPK and P-CREB in control group and transplantation group were lower than those in model group, positive control group and agonist group (P < 0.05) without significant difference\u0000 among other groups (P > 0.05). In conclusion, BMSCs can reduce the accumulation of extracellular matrix and glomerular sclerosis, thereby controlling FSGS progress.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45543522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fracture healing is an extremely complex physiological process, involving a sequence of crucial mechanisms. Whether zoledronic acid (ZA) affects proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and tibial fractures still remains unknown. We performed construction of the tibial fracture model in rats and analyzed the impact of ZA and ZA+LY294002 on fracture healing in rats. Test of the influence of ZA and ZA+LY294002 on crucial osteogenic genes alkaline phosphatase (ALP), RUNX2, OCN and OPN was performed. Examination of the influence of suppressing the PI3K/AKT pathway on the proliferation with bone differentiation of ZA. Results showed ZA distinctly accelerated the proliferation and ALP activity of BMSC cells, BMP2, RUNX2, OCN, OPN and the activation of the PI3K/AKT pathway. Repression of PI3K/AKT pathway suppressed the proliferation and osteogenic differentiation action of ZA. ZA boosted tibial fracture healing in rats via activating the PI3K/AKT pathway. ZA facilitates the proliferation with osteogenic differentiation of BMSC cells and tibial fracture healing in rats via activating the PI3K/AKT pathway.
{"title":"The Influence of Zoledronic Acid on the Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Tibial Fracture in Rats via Phosphatidylinositol-3-Kinase/AKT Pathway","authors":"Jianzhou Liu, Hao Wang, GuoJun Shang, Xiangyang Lv, Zhenwei Xu, Fujun Xiong","doi":"10.1166/jbt.2023.3258","DOIUrl":"https://doi.org/10.1166/jbt.2023.3258","url":null,"abstract":"Fracture healing is an extremely complex physiological process, involving a sequence of crucial mechanisms. Whether zoledronic acid (ZA) affects proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and tibial fractures still remains unknown. We\u0000 performed construction of the tibial fracture model in rats and analyzed the impact of ZA and ZA+LY294002 on fracture healing in rats. Test of the influence of ZA and ZA+LY294002 on crucial osteogenic genes alkaline phosphatase (ALP), RUNX2, OCN and OPN was performed. Examination of the influence\u0000 of suppressing the PI3K/AKT pathway on the proliferation with bone differentiation of ZA. Results showed ZA distinctly accelerated the proliferation and ALP activity of BMSC cells, BMP2, RUNX2, OCN, OPN and the activation of the PI3K/AKT pathway. Repression of PI3K/AKT pathway suppressed the\u0000 proliferation and osteogenic differentiation action of ZA. ZA boosted tibial fracture healing in rats via activating the PI3K/AKT pathway. ZA facilitates the proliferation with osteogenic differentiation of BMSC cells and tibial fracture healing in rats via activating the PI3K/AKT pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48427499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lu‐lu Chen, Feng Su, Si-nan Li, Xin Yi, Yuan Luo, Dong Liang, Zhen-chuan Tang, Chao Feng, Kai Cheng, Fang Deng
Objective: To observe Paeonol effects on repair of cerebral cortex in young rats with CP. Methods: Dividing CP model rats as Model and Pae groups, and a sham operation control group was set up. Using Nissl staining to evaluate morphology and number of cortical neurons in young rats, and to determine the protein of BDNF/TrkB in peripheral serum and cerebral cortex. Results: The number of normal morphological neurons in cerebral cortex of young rats in Pae group were more than that of Model group (P < 0.01). BDNF and TrkB concentrations were significantly up-regulation in serum in Model and Pae groups (P < 0.001), meanwhile, The BDNF and TrkB concentrations significantly up-regulated in serum in Pae group (P < 0.01). By IHC and WB assay, BDNF and TrkB protein levels were significantly up-regulation in serum in Model and Pae groups (P < 0.001), meanwhile, The BDNF and TrkB protein levels were significantly up-regulation in serum in Pae group (P < 0.01). Conclusion: Pae can all play a role in repairing cerebral cortex damage in young rats with cerebral palsy via BDNF/TrkB.
{"title":"Paeonol Effects on Repair of Cerebral Cortex Injury and the Expression of Brain-Derived Neurotrophic Factor/Tropomyosin Receptor Kinase B in Postnatal Rats with Cerebral Palsy","authors":"Lu‐lu Chen, Feng Su, Si-nan Li, Xin Yi, Yuan Luo, Dong Liang, Zhen-chuan Tang, Chao Feng, Kai Cheng, Fang Deng","doi":"10.1166/jbt.2023.3260","DOIUrl":"https://doi.org/10.1166/jbt.2023.3260","url":null,"abstract":"Objective: To observe Paeonol effects on repair of cerebral cortex in young rats with CP. Methods: Dividing CP model rats as Model and Pae groups, and a sham operation control group was set up. Using Nissl staining to evaluate morphology and number of cortical neurons\u0000 in young rats, and to determine the protein of BDNF/TrkB in peripheral serum and cerebral cortex. Results: The number of normal morphological neurons in cerebral cortex of young rats in Pae group were more than that of Model group (P < 0.01). BDNF and TrkB concentrations were\u0000 significantly up-regulation in serum in Model and Pae groups (P < 0.001), meanwhile, The BDNF and TrkB concentrations significantly up-regulated in serum in Pae group (P < 0.01). By IHC and WB assay, BDNF and TrkB protein levels were significantly up-regulation in serum\u0000 in Model and Pae groups (P < 0.001), meanwhile, The BDNF and TrkB protein levels were significantly up-regulation in serum in Pae group (P < 0.01). Conclusion: Pae can all play a role in repairing cerebral cortex damage in young rats with cerebral palsy via BDNF/TrkB.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49198191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chi Hyung Lee, J. Kwak, S. Sung, Sang Weon Lee, J. Kwak, Kyung-wan Kim, H. Kang, G. Kim
DOPC liposome properties of various sizes were analyzed via atomic force microscopy (AFM) and patch-clamp. The unilamellarity of small DOPC liposome (below 1 μm) was confirmed via Cryogenic transmission electron microscopy (Cryo-TEM). Small DOPC liposome (below 1 μm) showed a bending modulus (kbend) ranging (between 10−18 and 10−20 J). The bending modulus value was a size dependence. In order words, it decreased as DOPC liposome size increased. For DOPC liposome (above 1 μm), patch-clamp was used to achieve electrically tight whole-cell configurations. Our result showed that the unilamellar DOPC liposome (above 1 μm) exhibit RC circuit response property. It was similar to theoretical values (τ = 4.52 ms) of the unilamellar liposome. In this study, the uniform lamellarity of the DOPC liposome of various sizes was confirmed through electrical and mechanical properties of DOPC liposome.
{"title":"Unilamellar Characteristic Analysis of 1,2-dioleoyl-sn-3-phosphocholine Liposome of Various Sizes via Atomic Force Microscopy and Patch-Clamp","authors":"Chi Hyung Lee, J. Kwak, S. Sung, Sang Weon Lee, J. Kwak, Kyung-wan Kim, H. Kang, G. Kim","doi":"10.1166/jbt.2023.3263","DOIUrl":"https://doi.org/10.1166/jbt.2023.3263","url":null,"abstract":"DOPC liposome properties of various sizes were analyzed via atomic force microscopy (AFM) and patch-clamp. The unilamellarity of small DOPC liposome (below 1 μm) was confirmed via Cryogenic transmission electron microscopy (Cryo-TEM). Small DOPC liposome (below 1 μm)\u0000 showed a bending modulus (kbend) ranging (between 10−18 and 10−20 J). The bending modulus value was a size dependence. In order words, it decreased as DOPC liposome size increased. For DOPC liposome (above 1 μm), patch-clamp was\u0000 used to achieve electrically tight whole-cell configurations. Our result showed that the unilamellar DOPC liposome (above 1 μm) exhibit RC circuit response property. It was similar to theoretical values (τ = 4.52 ms) of the unilamellar liposome. In this study, the uniform\u0000 lamellarity of the DOPC liposome of various sizes was confirmed through electrical and mechanical properties of DOPC liposome.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47164558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Osteoarthritis (OA) is a degenerative joint disease due to the limited capacity to repair itself. There is an increasing need for novel and more effective alternatives to promote cartilage joint regeneration. Natural compounds as herbal remedies are ideal to be considered to treat OA. In order to explore the functional herbal remedy, we investigated the efficacy of herbal mixture along with bone mesenchymal stem cells (BMSCs) in repairing rat cartilage tissues. Forty SD rats were randomly divided into four groups. A cartilage injury models by a drilling was made. The histological H&E analysis, Mankin scores and cartilage-specific markers were tested. We found that herbal mixture treatment can significantly improve the damaged cartilage compared to the control. Moreover, the combination of herbal formulation and 3D bioscaffold containing BMSCs can produce better efficacy to repair the damaged cartilages. Our data provides that herbal formulation is effective to treat damaged cartilage, and the herbal remedy along with BMSCs is most promising therapeutic effect in repairing damaged cartilage tissue, demonstrating a combinational therapeutic effect to be considered in the clinic.
{"title":"The Therapeutic Effect of Herbal Mixture in Repairing Degenerated Joint","authors":"Haidong Wang, Xiao‐dong Yao, Chengjun Wu","doi":"10.1166/jbt.2023.3238","DOIUrl":"https://doi.org/10.1166/jbt.2023.3238","url":null,"abstract":"Osteoarthritis (OA) is a degenerative joint disease due to the limited capacity to repair itself. There is an increasing need for novel and more effective alternatives to promote cartilage joint regeneration. Natural compounds as herbal remedies are ideal to be considered to treat OA.\u0000 In order to explore the functional herbal remedy, we investigated the efficacy of herbal mixture along with bone mesenchymal stem cells (BMSCs) in repairing rat cartilage tissues. Forty SD rats were randomly divided into four groups. A cartilage injury models by a drilling was made. The histological\u0000 H&E analysis, Mankin scores and cartilage-specific markers were tested. We found that herbal mixture treatment can significantly improve the damaged cartilage compared to the control. Moreover, the combination of herbal formulation and 3D bioscaffold containing BMSCs can produce better\u0000 efficacy to repair the damaged cartilages. Our data provides that herbal formulation is effective to treat damaged cartilage, and the herbal remedy along with BMSCs is most promising therapeutic effect in repairing damaged cartilage tissue, demonstrating a combinational therapeutic effect\u0000 to be considered in the clinic.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49296734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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