Journal of chemical technology and biotechnology最新文献

{"title":"Performance of immobilized recombinant Escherichia coli on β-cyclodextrin glucanotransferase secretion and cell viability in a membrane bioreactor","authors":"Nurul Nabila Huda Baharudin,&nbsp;Nor Hasmaliana Abdul Manas,&nbsp;Rohaida Che Man,&nbsp;Rosli Md Illias,&nbsp;Siti Kholijah Abdul Mudalip,&nbsp;Siti Zubaidah Sulaiman","doi":"10.1002/jctb.70037","DOIUrl":"https://doi.org/10.1002/jctb.70037","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>The efficiency of recombinant enzyme secretion into the medium is essential for simplifying purification and increasing yield. However, <i>Escherichia coli</i>, a commonly used expression host, faces challenges such as cell lysis and plasmid instability during enzyme secretion. Cell immobilization is proposed to address this problem. This study aimed to investigate the performance of immobilized recombinant <i>E. coli</i> focusing on <i>β</i>-cyclodextrin glucanotransferase (<i>β</i>-CGTase) secretion, cell lysis and plasmid stability in a membrane bioreactor. The study compared two immobilization configurations, IC-9 (9 hollow-fiber membranes) and IC-19 (19 hollow-fiber membranes), as well as a shake flask system.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>IC-9 exhibited superior performance, achieving 1.5-fold higher <i>β</i>-CGTase activity than IC-19. Additionally, IC-9 showed 87% lower cell lysis and 12% higher plasmid stability compared to IC-19, indicating improved process reliability. The immobilized cells in the membrane bioreactor exhibited 2.5-fold higher <i>β</i>-CGTase secretion, 75% lower cell lysis and 29% higher plasmid stability compared to the shake flask system. Moreover, <i>β</i>-CGTase secretion by immobilized cells was twice as high as that of free cells and remained stable over seven cycles, achieving a cumulative <i>β</i>-CGTase activity of 1120.21 U mL⁻¹. Kinetic analysis showed that immobilized cells exhibited a lower maximum specific growth rate (0.012 h^−<b>1</b>) compared to free cells (0.149 h^−<b>1</b>), but significantly higher <i>β</i>-CGTase secretion, with an <i>α</i> (growth-associated production coefficient) of 1.317 U mg⁻¹ mL⁻¹ compared to 0.004 U mg⁻¹ mL⁻¹ for free cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>These results highlight the potential of membrane bioreactors for large-scale recombinant enzyme production with improved efficiency and operational stability. © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 12","pages":"2689-2699"},"PeriodicalIF":2.4,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145469758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monoethanolamine deep eutectic solvent on isolation of cardanol from cashew nutshell liquid","authors":"Anantharaj Ramalingam,&nbsp;Vichitra Malaiyarasan,&nbsp;Gayathri Mahavishnu","doi":"10.1002/jctb.70034","DOIUrl":"10.1002/jctb.70034","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Monoethanolamine-based deep eutectic solvent (MEADES) at different molar ratios (1:2, 1:3, 1:4, 1:5, 1:6, and 1:7) was prepared and used for the isolation of cardanol from cashew nutshell liquid (CNSL).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>In this work, the density of MEADES at different molar ratios (1:2, 1:3, 1:4, 1:5, 1:6, and 1:7), isobutyl methyl ketone (IBMK), ethyl acetate (EtAc), acetone, hexane, methanol, ethanol, and butanol was measured at different temperatures (293.15–343.15 K). Subsequently, the density of the binary mixture of IBMK with EtAc over the whole composition range was measured at different temperatures (293.15–343.15 K). Further, the pH of all the prepared MEADES was determined and functional group analysis was carried out using Fourier transform infrared (FTIR) spectroscopy. Finally, the experimental isolation of cardanol was conducted with feed:ternary mixtures at different solvent ratios (50:150, 50:125, 5:100, and 50:75 mL) and characterized using FTIR spectroscopy and gas chromatography–mass spectrometry.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>It was observed that all the prepared deep eutectic solvents have strong hydrogen bonds between choline chloride (ChCl) and monoethanolamine (MEA) at all the studied molar ratios when the wavenumbers of individual compounds with mixtures of ChCl and MEA were compared. All the prepared MEADES used for isolation of cardanol from CNSL by varying the feed:ternary solvent ratios. It was concluded that the percentage of cardanol isolation was strongly dependent on the molar ratio of ChCl and MEA, and the volume of IBMK and EtAc as cosolvent in their mixtures. 98.93% cardanol isolation was achieved when using a 50 mL CNSL:75 mL mixture of solvents (i.e., 25 mL MEADES:25 mL IBMK:25 mL EtAc). © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2200-2207"},"PeriodicalIF":2.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of biochar for enhancing methane production and alleviating sulfide toxicity in skim latex wastewater with minimized roles of biochar as a carbon source","authors":"Sengthong Lee,&nbsp;Nuntiya Paepatung,&nbsp;Pornpan Panichnumsin,&nbsp;Keonakhone Khounvilay,&nbsp;Norazwina Zainol,&nbsp;Chantaraporn Phalakornkule","doi":"10.1002/jctb.70033","DOIUrl":"https://doi.org/10.1002/jctb.70033","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>The conversion of organic matter in sulfate-rich wastewater to methane presents a significant challenge due to the competition between methanogenesis and sulfidogenesis. In this study, we investigated the potential of <i>Earleaf acacia</i>-based biochar (EA-biochar) to enhance methane production and alleviate sulfide toxicity in skim latex wastewater (SLW). The role of biochar as an extra carbon source was minimized by washing it three times before use. Anaerobic digestion of SLW was investigated under both batch and fed-batch operational modes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The methane yield of the batch with EA-biochar addition was 252.85 ± 5.37 mL g⁻¹ COD, significantly higher than that of the batch without EA-biochar (192.93 ± 5.52 mL g⁻¹ COD). In addition, the methane production rate of the batch with EA-biochar was 25% higher than that of the batch without EA-biochar addition. The increased methane yield was correlated with lower levels of volatile fatty acids and total organic carbon in the media, highlighting the role of EA-biochar in mediating electron transfer for methanogenesis rather than providing additional carbon sources.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>The results from the fed-batch operation were consistent with those from the batch operation, indicating that the biochar effectively promoted methanogenesis for at least 80 days. © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 12","pages":"2575-2584"},"PeriodicalIF":2.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145469458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulation of a biorefinery process from the organic fraction of municipal solid waste generated in Mexico City","authors":"Arturo Pérez-Roman,&nbsp;Teresa Lopez-Arenas,&nbsp;Mauricio Sales-Cruz,&nbsp;José Rocha-Rios","doi":"10.1002/jctb.70018","DOIUrl":"10.1002/jctb.70018","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>In this work, a biorefinery process was designed and simulated in Aspen Plus® V.10 for production of biogas, bioethanol, and biofertilizer from the Organic Fraction of Municipal Solid Waste (OFMSW) generated in Mexico City (CDMX). The process consists of five stages: (i) anaerobic digestion, (ii) acid hydrolysis, (iii) simultaneous saccharification and co-fermentation, (iv) conventional distillation, and (v) dehydration by extractive distillation. Stirred tank and stoichiometric reactors were used for the anaerobic biodigester simulation, while stoichiometric reactors were employed for simulation of the acid hydrolysis, and simultaneous saccharification and co-fermentation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The overall results of the simulated process show that it is possible to obtain 149.6 Tons of Biogas, 41.9 Tons of Biofertilizer and 19.7 Tons of Bioethanol from 1000 Tons of OFMSW processed daily, which is equivalent to a total yield of 0.21 Ton Bioproducts/Ton OFMSW or 0.947 Ton Bioproducts/Ton Volatile Solids contained in the OFMSW.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSIONS</h3>\u0000 \u0000 <p>The conceptual design and simulation of the biorefinery process presented in this work indicate that it is technically feasible to obtain 3 different bioproducts from the OFMSW generated in CDMX. Computer-aided process design will allow progress towards the circular economy, where biorefineries will play a leading role. © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2053-2064"},"PeriodicalIF":2.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Fenton and Fered-Fenton processes for sustainable management of textile reverse osmosis concentrate: multivariate analysis approach","authors":"Gamze Canbakal,&nbsp;Öykü Nur Ersoz,&nbsp;Gulay Arslan Cene,&nbsp;Senem Yazici Guvenc,&nbsp;Emine Can-Güven,&nbsp;Gamze Varank","doi":"10.1002/jctb.70030","DOIUrl":"10.1002/jctb.70030","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Water scarcity and increasingly stringent discharge regulations are driving the adoption of sustainable water management strategies, necessitating the reuse of industrial wastewater and the implementation of zero or near-zero liquid discharge systems. Reverse osmosis (RO) is an effective technology for recycling wastewater in the textile industry. The elevated resistivity and decreased biodegradability of contaminants in RO concentrates generate a novel wastewater stream.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This study examines the reutilization of these wastewaters through Fenton and Fered-Fenton processes. The Box–Behnken design (BBD) was used to model process variables. The operational parameters for both processes were the H₂O₂/chemical oxygen demand (COD) ratio, Fe²⁺/H₂O₂ ratio, current density and reaction time. The evaluated system responses were COD, color index (CI) and ultraviolet absorbance at 254 nm (UV₂₅₄) and 280 nm (UV₂₈₀). The correlation coefficients (<i>R</i>²) for all created models approached 1, demonstrating the efficacy of the BBD method in modeling pollutant removal with the Fenton and Fered-Fenton processes. The Fenton process achieved removal efficiency of 73.5% for COD, 94.5% for CI, 84% for UV₂₅₄ and 81% for UV₂₈₀. The efficiencies obtained in the Fered-Fenton process were 87% for COD, 97.5% for CI, 94.5% for UV₂₅₄ and 89% for UV₂₈₀ under optimum conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The results indicate that the modified Fenton process may be a more successful alternative method, notably owing to its enhanced removal efficiencies for organic matter. © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2187-2199"},"PeriodicalIF":2.4,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vanadium removal and recovery from aqueous solution with repeated use of a KOH-modified seaweed biochar adsorbent: characterisation and removal mechanisms","authors":"Bashir M Ghanim,&nbsp;Ronan Courtney,&nbsp;J Tony Pembroke,&nbsp;James J Leahy,&nbsp;Thomas F O'Dwyer,&nbsp;John G Murnane","doi":"10.1002/jctb.70015","DOIUrl":"10.1002/jctb.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Vanadium (V) is a critical raw material which is extensively used in metallurgical, aerospace and chemical industries. However, V-enriched wastewaters pose risks to human and environmental health due to their tendency to persist and bioaccumulate. Active V treatment processes generate significant quantities of byproducts with associated high operating costs and there is a need therefore to investigate novel passive technologies such as biosorption. This study investigates the capacity and reusability of KOH-modified seaweed biochar (BC_KOH) to (re-)adsorb, desorb and recover V, and evaluates the influences of initial V concentration, contact time, solution temperature and pH.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The maximum uptake of 48.8 mg V g⁻¹ BC_KOH occurred within 75 min and followed an exothermic adsorption process best described by the Langmuir isotherm model. The magnitude of enthalpy change suggested a physisorption binding interaction with optimum uptake in the range pH 3.5–4.5. Introduction of a saline content (100–400 mg Na⁺ L⁻¹) into the adsorption solution resulted in a modest reduction in the V adsorption level by BC_KOH but further increases in saline concentration thereafter had only limited impact. Successive V(V) adsorption/desorption cycles indicated that V(V) binding to BC_KOH displays effective reversibility with the adsorbent material demonstrating good regeneration characteristics.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>BC_KOH offers clear potential to be used as a cost-effective passive and robust adsorbent of V within a range of acidic industrial waste streams. The capacity of the biochar to desorb and re-adsorb demonstrates high V recovery potential and excellent biochar regeneration capability. © 2025 The Author(s). <i>Journal of Chemical Technology and Biotechnology</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2039-2052"},"PeriodicalIF":2.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://scijournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous stirred tank reactor with recycled iron mixer for Fe(II)/chlorine-driven textile dyes oxidation: toward a sustainable continuous-flow process for micropollutants degradation","authors":"Slimane Merouani,&nbsp;Abdelkader Sigha,&nbsp;Hasan A. M. Hussein,&nbsp;Sadi M. Y. Almajdalawi","doi":"10.1002/jctb.70031","DOIUrl":"10.1002/jctb.70031","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>This study presents a novel and sustainable continuous-flow process for micropollutants degradation, using a lab-scale continuous stirred tank reactor (CSTR) equipped with a recycled iron mixer to drive Fe(II)/chlorine-induced oxidation. The recycled iron rod functions both as a mechanical stirrer and an in-situ source of Fe(II). The system's performance was evaluated by studding parameters such as chlorine flow rate (40–300 μL/s), submerged rod length (1–8 cm), rotation speed (0–500 rpm), dye concentration (5–40 mg/L), and pH (3–6).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULT</h3>\u0000 \u0000 <p>Maximum dye removal of 74% was achieved at pH 3, with higher chlorine flow rates, longer submerged rod length, and faster rotation speeds for an inlet dye concentration of 20 mg/L, while the reaction residence time did not exceed 45 s. Over 90% dye removal was observed at low concentrations (5 mg/L), and optimal performance occurred under acidic pH conditions. Higher pH values inhibited iron corrosion, quenching Fe(II) release and nullifying degradation. The matrix effect showed minimal impact with mineral water, while river and seawater reduced efficiency. Scavenger tests indicated ferryl species (Fe(IV)) as primary oxidant, while hydroxyl and chlorine radicals contributing less (10–20%). A comparison with a conventional Fe(II)/chlorine process showed that while the pre-dissolved Fe(II) system achieved higher conversion rates, the recycled iron system offered greater sustainability and operational simplicity through continuous Fe(II) generation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>This process provides a cost-effective, environmentally friendly, and efficient solution for micropollutant degradation in water matrices with light mineral and organic charges. © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2176-2186"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An appraisal on various hydrogen productions and their CO2 emissions – a comparison on the Grey, blue and green pathways","authors":"Stephen Truslove,&nbsp;Elliott L. Bruce,&nbsp;Humphrey H. P. Yiu","doi":"10.1002/jctb.70029","DOIUrl":"10.1002/jctb.70029","url":null,"abstract":"<p>The transition to a net-zero economy requires revolutionary change to the fundamentals of economies around the world. The need to decarbonise processes affects almost every aspect of industry and society, with energy, transport, and manufacturing making up some of the most polluting sectors. Many complementary solutions must be realised to surmount such challenges, and one suggestion is the greater use of hydrogen as a fuel or feedstock. For this to occur, hydrogen production must be significantly scaled up without introducing so many emissions as to counteract improvements made downstream. Although hydrogen gas can be found in underground deposits, almost all hydrogen is produced industrially from fossil fuels, notably <i>via</i> steam reforming process (grey hydrogen), which is usually associated with a considerable carbon footprint. Alternative production pathways with a lower carbon footprint have been developed for large-scale production. This perspective examines the impact of large-scale grey, blue, and green hydrogen production methods and their viability as sources of a cleaner fuel for heat and electricity generation. © 2025 Society of Chemical Industry (SCI).</p>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 9","pages":"1753-1761"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced downstream process design for recovery of 2-phenylethanol and 2-phenylethyl acetate after biotransformation","authors":"Tamara Janković,&nbsp;Adrie JJ Straathof,&nbsp;Anton A Kiss","doi":"10.1002/jctb.70027","DOIUrl":"10.1002/jctb.70027","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>2-Phenylethanol (2-PE) and 2-phenylethyl acetate (2-PEAc) are valuable aroma compounds with growing market demands. As an alternative to conventional petrochemical production, more valuable natural forms of these chemicals can be obtained by biotransformation. Low product concentrations, resulting from significant product toxicity to microorganisms, and high boiling points of products complicate recovery process. <i>In situ</i> product recovery by liquid–liquid extraction can be used to increase bioprocess yield and productivity. However, the subsequent purification of 2-PE and 2-PEAc is challenging as a consequence of the multiple phases, high-boiling temperatures of main products, occurrence of remaining substrate and byproducts, and presence of microorganisms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The main goal of this original work is to improve the competitiveness of the biotechnological production of 2-PE by using <i>in silico</i> methods to develop an advanced industrial process for the final purification after centrifugation. An adaptable dividing-wall column was designed to remove 2-PE with 2-PEAc from organic phase or to esterify 2-PE to pure 2-PEAc. The production flexibility of the developed process allows adjustability to market demand. Additionally, recovery of co-produced ethanol from aqueous phase can increase the economic and environmental performance of the developed process. As confirmed by detailed techno-economic analysis, the proposed processes can cost-effectively (total recovery costs of 0.64–0.72 US$/kg_2-PE/2-PEAc) and energy-efficiently (primary energy requirements of 1.83–2.05 kW_thh/kg_2-PE/2-PEAc) recovery of 2-PE or 2-PEAc after biotransformation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>The developed process enhances economic and environmental viability of biotechnological 2-PE production by reducing costs and energy requirements, while ensuring flexibility to adapt to market demands. © 2025 The Author(s). <i>Journal of Chemical Technology and Biotechnology</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2019-2028"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://scijournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of CNF-TiO2 composites and process parameters on DMFC performance enhancement","authors":"Muhammad Syafiq Alias,&nbsp;Siti Kartom Kamarudin,&nbsp;Wei Lun Ang,&nbsp;Mohd Shahbudin Masdar,&nbsp;Norilhamiah Yahya,&nbsp;Nabila A. Karim","doi":"10.1002/jctb.70021","DOIUrl":"10.1002/jctb.70021","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Direct methanol fuel cells (DMFCs) face challenges from methanol crossover and performance degradation caused by inefficient microporous layer (MPL) designs, where conventional carbon-based MPLs show limitations in surface coverage uniformity, catalyst utilization efficiency, and pore structure balance. To address these issues, this study developed an optimized MPL using carbon nanofiber-titanium dioxide (CNF-TiO₂) composites through parametric optimization of the fabrication process. Key variables included MPL loading density, CNF:TiO₂ mass ratio, Nafion binder concentration, and methanol feed concentration, which were analyzed <i>via</i> screening experiments and Response Surface Methodology (RSM) using a Central Composite Design (CCD). The optimization targeted three response metrics: power density (R1), open-circuit voltage (R2), and peak current density (R3).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>The results demonstrated that the modified CNF-TiO₂ microporous layer (MPL) exhibited a synergistic effect when optimized with the selected parameters. Initial screening experiments examined MPL loading, CNF-to-TiO₂ ratio, Nafion® content, and methanol concentration, revealing that the composition ratio, Nafion® content, and methanol concentration had significant impacts on the cell's performance responses. These key parameters were further explored using Response Surface Methodology (RSM). The optimized MPL exhibited improved surface coverage and promoted pore development, resulting in 1.22% microporosity and 34.31% mesoporosity. As a result, the composite MPL enhanced peak power density by 55%, reaching 70.43 mW cm⁻². Analysis of variance (ANOVA) confirmed that the improvements in power density, open-circuit voltage, and optimal current density were statistically significant, highlighting the effectiveness of the composite MPL design and process optimization in enhancing DMFC performance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>These findings highlight the essential need to integrate nanocomposite engineering with parametric optimization to achieve coverage uniformity, catalyst utilization efficiency, and pore structure balance. The research presents a verified methodology for multi-objective optimization in DMFCs, enhancing scalable, high-performance fuel cell systems for sustainable energy applications. © 2025 Society of Chemical Industry (SCI).</p>\u0000 </section>\u0000 </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2103-2117"},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}