Journal of Clinical Periodontology最新文献_第9页

Association between masticatory function and depression in older adults: Results from NHANES 2009 to 2018 老年人咀嚼功能与抑郁之间的关系：2009 年至 2018 年美国国家健康调查（NHANES）结果。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-10 DOI: 10.1111/jcpe.14046

Xindi Wei, Xiaomeng Zhang, Xiao Zhang, Ruiying Chen, Maurizio S. Tonetti, Junyu Shi, Hongchang Lai

Aim

Depression is prevalent among older adults. Although the number of missing teeth is considered to be associated with depression, the relationship between masticatory function, which is usually indicated by functional tooth units (FTUs), and depression in older adults remains unclear.

Materials and Methods

This study used data from the National Health and Nutrition Examination Survey (NHANES) 2009–2018. The number of FTUs was defined as pairs of opposing natural and artificial teeth. Depression was accessed using the Patient Health Questionnaire (PHQ-9), and participants who scored ≥10 on PHQ-9 were diagnosed with depression. Logistic regression analyses, propensity score matching (PSM) analyses and subgroup analyses were conducted to assess the association between FTU and depression.

Results

The analysis included 5764 individuals over 60 years. An association between FTU and the risk of depression among older adults was detected (odds ratio [OR] = 0.951, 95% confidence interval [CI] 0.915–0.989), suggesting protective roles of more FTUs. Significant increase in the risk of depression in 0 ≤ FTUs ≤ 3 was observed compared with 10 ≤ FTUs ≤ 12 (OR = 1.819, 95% CI 1.157–2.858). However, no significant increase in the risk of depression in 4 ≤ FTUs ≤ 9 was found. After PSM, significant increase in the risk of depression in 0 ≤ FTUs ≤ 3 was still detected compared with 4 ≤ FTUs ≤ 12 (OR = 1.484, 95% CI 1.030–2.136). Subgroup analyses demonstrated consistent results in all subgroups, except for individuals aged 76–80 and drinking regularly.

Conclusions

The findings suggested the association between impaired masticatory function and the risk of depression among older adults. Longitudinal studies are needed to elucidate the role of masticatory function impairment in the development of depression further.

目的：抑郁症在老年人中很普遍。虽然缺牙数量被认为与抑郁有关，但通常以功能性牙齿单位（FTU）表示的咀嚼功能与老年人抑郁之间的关系仍不清楚：本研究使用了 2009-2018 年美国国家健康与营养调查（NHANES）的数据。FTU 的数量定义为对立的天然牙和人工牙。通过患者健康问卷（PHQ-9）了解抑郁情况，PHQ-9得分≥10分的参与者被诊断为抑郁症患者。研究人员进行了逻辑回归分析、倾向得分匹配（PSM）分析和亚组分析，以评估 FTU 与抑郁症之间的关系：分析对象包括 5764 名 60 岁以上的老年人。研究发现，FTU 与老年人患抑郁症的风险之间存在关联（几率比 [OR] = 0.951，95% 置信区间 [CI] 0.915-0.989），这表明更多的 FTU 具有保护作用。与 10 ≤ FTU ≤ 12 相比，0 ≤ FTU ≤ 3 患抑郁症的风险显著增加（OR = 1.819，95% 置信区间 [CI] 1.157-2.858）。然而，4 ≤ FTU ≤ 9 的抑郁风险没有明显增加。在 PSM 后，与 4 ≤ FTU ≤ 12 相比，0 ≤ FTU ≤ 3 的抑郁风险仍有明显增加（OR = 1.484，95% CI 1.030-2.136）。亚组分析表明，除了年龄在76-80岁之间且经常饮酒的人之外，所有亚组的结果都是一致的：研究结果表明，咀嚼功能受损与老年人患抑郁症的风险有关。要进一步阐明咀嚼功能受损在抑郁症发病中的作用，还需要进行纵向研究。

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引用次数: 0

Using SWATH-MS to identify new molecular biomarkers in gingival crevicular fluid for detecting periodontitis and its response to treatment 利用 SWATH-MS 在牙龈沟液中鉴定新的分子生物标记物，以检测牙周炎及其对治疗的反应。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-10 DOI: 10.1111/jcpe.14037

T. Blanco-Pintos, A. Regueira-Iglesias, M. Relvas, M. Alonso-Sampedro, M. P. Chantada-Vázquez, C. Balsa-Castro, I. Tomás

Aim

To identify new biomarkers to detect untreated and treated periodontitis in gingival crevicular fluid (GCF) using sequential window acquisition of all theoretical mass spectra (SWATH-MS).

Materials and Methods

GCF samples were collected from 44 periodontally healthy subjects and 40 with periodontitis (Stages III–IV). In the latter, 25 improved clinically 2 months after treatment. Samples were analysed using SWATH-MS, and proteins were identified by the UniProt human-specific database. The diagnostic capability of the proteins was determined with generalized additive models to distinguish the three clinical conditions.

Results

In the untreated periodontitis vs. periodontal health modelling, five proteins showed excellent or good bias-corrected (bc)-sensitivity/bc-specificity values of >80%. These were GAPDH, ZG16B, carbonic anhydrase 1, plasma protease inhibitor C1 and haemoglobin subunit beta. GAPDH with MMP-9, MMP-8, zinc-α-2-glycoprotein and neutrophil gelatinase-associated lipocalin and ZG16B with cornulin provided increased bc-sensitivity/bc-specificity of >95%. For distinguishing treated periodontitis vs. periodontal health, most of these proteins and their combinations revealed a predictive ability similar to previous modelling. No model obtained relevant results to differentiate between periodontitis conditions.

Conclusions

New single and dual GCF protein biomarkers showed outstanding results in discriminating untreated and treated periodontitis from periodontal health. Periodontitis conditions were indistinguishable. Future research must validate these findings.

目的：利用所有理论质谱的顺序窗口采集（SWATH-MS）技术，确定检测牙龈缝隙液（GCF）中未治疗和已治疗牙周炎的新生物标记物：采集了 44 名牙周健康者和 40 名牙周炎患者（III-IV 期）的 GCF 样本。后者中有 25 人在治疗 2 个月后临床症状有所改善。使用 SWATH-MS 对样本进行分析，并通过 UniProt 人类特异性数据库对蛋白质进行鉴定。利用广义加性模型确定了蛋白质的诊断能力，以区分三种临床状况：结果：在未经治疗的牙周炎与牙周健康模型中，有五种蛋白质的灵敏度/特异度偏差校正（bc）值大于80%，显示出极佳或良好的灵敏度/特异度偏差校正（bc）值。它们是 GAPDH、ZG16B、碳酸酐酶 1、血浆蛋白酶抑制剂 C1 和血红蛋白亚基 beta。GAPDH与MMP-9、MMP-8、锌-α-2-糖蛋白和中性粒细胞明胶酶相关脂褐质以及ZG16B与玉米蛋白的bc敏感性/bc特异性均大于95%。在区分治疗过的牙周炎与牙周健康方面，这些蛋白质及其组合的预测能力与以前的模型相似。在区分牙周炎条件方面，没有任何模型能获得相关结果：结论：新的单一和双重 GCF 蛋白生物标志物在区分未经治疗和已治疗的牙周炎与牙周健康方面表现突出。牙周炎的情况无法区分。未来的研究必须验证这些发现。

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引用次数: 0

A nomogram-based predictive model for tooth survival in Chinese patients with periodontitis: An 11-year retrospective cohort study 基于提名图的中国牙周炎患者牙齿存活率预测模型：一项为期 11 年的回顾性队列研究。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-10 DOI: 10.1111/jcpe.14027

Shuwen Shi, Yang Meng, Jian Jiao, Dong Shi, Xianghui Feng, Huanxin Meng

Aim

To develop a nomogram-based predictive model of tooth survival by comprehensively analysing clinical and radiographic risk factors of tooth loss (TL).

Materials and Methods

In this study, 3447 teeth of 131 subjects who underwent non-surgical periodontal treatment were examined retrospectively within a mean follow-up period of 11.6 years. The association of risk factors including clinical and radiographic parameters with TL was assessed using univariate and multivariate Cox regression analyses. A nomogram-based predictive model was developed, and its validation and discriminatory ability were analysed.

Results

In all, 313 teeth were lost in 94 patients in this study (overall tooth loss [OTL] 9.08%; 0.21 teeth/patient/year). Male, heavy smoking, molar teeth, probing depth (PD), attachment loss (AL), tooth mobility and radiographic bone loss were significantly associated with TL (p < .05). A gradient effect of tooth mobility on TL increased from degree I to III versus none (p < .0001). The area under the curve (AUC) of the model was 0.865. Calibration curve and decision curve analysis demonstrated good performance and high net benefit, respectively.

Conclusions

Adopting a specific nomogram could facilitate the prediction of tooth survival and the development of tailored treatment plans in Chinese patients with advanced periodontitis.

目的：通过全面分析牙齿脱落（TL）的临床和放射学风险因素，建立基于提名图的牙齿存活率预测模型：本研究对 131 名接受过非手术牙周治疗的受试者的 3447 颗牙齿进行了回顾性检查，平均随访时间为 11.6 年。使用单变量和多变量 Cox 回归分析评估了包括临床和放射学参数在内的风险因素与 TL 的关系。研究还建立了一个基于提名图的预测模型，并对其验证和判别能力进行了分析：本研究中共有 94 名患者的 313 颗牙齿脱落（总牙齿脱落率 [OTL] 9.08%；0.21 颗/患者/年）。男性、大量吸烟、磨牙、探诊深度 (PD)、附着丧失 (AL)、牙齿移动性和放射骨丧失与 TL 显著相关（p 结论：采用特定的提名图可以帮助确定牙齿缺失的程度：采用特定的提名图有助于预测中国晚期牙周炎患者的牙齿存活率并制定有针对性的治疗方案。

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引用次数: 0

Association of the Life's Essential 8 cardiovascular health score with periodontitis among US adults 美国成年人生活必备 8 项心血管健康评分与牙周炎的关系。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-04 DOI: 10.1111/jcpe.14042

Lang Liang, Chia-Yu Chen, Izzuddin M. Aris

Aim

To investigate whether cardiovascular health (CVH) is associated with periodontitis.

Materials and Methods

We used data from the 2009 to 2014 National Health and Nutrition Examination Survey. We quantified CVH using Life's Essential 8, which includes four health behaviours (diet, smoking, physical activity and sleep) and four health factors (body mass index, blood cholesterol, glucose and pressure). We categorized scores as low (0–49), moderate (50–79) and high (80–100). We calculated subscores of health behaviours and factors and categorized them as low, moderate and high. We used logistic regression to assess the association of CVH with periodontitis, adjusting for age, gender, race/ethnicity, education, poverty index, marital status and health insurance. We computed odds ratios (ORs) and 95% confidence intervals (CIs).

Results

This study included 9296 adults ≥30 years old. Multivariable-adjusted models showed that subjects with moderate (OR, 0.62; 95% CI: 0.52–0.74) or high (OR, 0.43; 95% CI: 0.33–0.57) CVH had significantly lower odds of periodontitis compared to those with low CVH. These results were consistent in the health behaviours model, but the estimates in the health factors model were not significant.

Conclusions

Improving CVH may help prevent periodontitis. Longitudinal studies are needed to confirm our findings.

目的：研究心血管健康（CVH）是否与牙周炎有关：我们使用了 2009 年至 2014 年全国健康与营养调查的数据。我们使用 "生活必备 8 要素 "对心血管健康进行量化，其中包括四种健康行为（饮食、吸烟、体育锻炼和睡眠）和四种健康因素（体重指数、血胆固醇、血糖和血压）。我们将得分分为低分（0-49 分）、中分（50-79 分）和高分（80-100 分）。我们计算了健康行为和因素的子分数，并将其分为低、中和高。我们使用逻辑回归评估了 CVH 与牙周炎的关系，并对年龄、性别、种族/民族、教育程度、贫困指数、婚姻状况和医疗保险进行了调整。我们计算了几率比（ORs）和 95% 的置信区间（CIs）：本研究共纳入 9296 名年龄≥30 岁的成年人。多变量调整模型显示，与低 CVH 受试者相比，中度（OR，0.62；95% CI：0.52-0.74）或高度（OR，0.43；95% CI：0.33-0.57）CVH 受试者患牙周炎的几率明显较低。这些结果在健康行为模型中是一致的，但在健康因素模型中的估计值并不显著：结论：改善CVH有助于预防牙周炎。需要进行纵向研究来证实我们的发现。

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引用次数: 0

Association between biological ageing and periodontitis: Evidence from a cross-sectional survey and multi-omics Mendelian randomization analysis 生物老化与牙周炎之间的关系：横断面调查和多组学孟德尔随机分析的证据。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-02 DOI: 10.1111/jcpe.14040

Zhendong Huang, Simin Peng, Ting Cen, Xiaoxuan Wang, Li Ma, Zhengguo Cao

Aim

To investigate the relationship and potential causality between biological ageing and periodontitis.

Materials and Methods

We obtained the National Health and Nutrition Examination Survey (NHANES) and genome-wide association study (GWAS) summary statistics as well as single-cell sequencing data. Multivariate regression analysis based on cross-sectional data, Mendelian randomization (MR) and multi-omics integration analysis were employed to explore the causal association and potential molecular mechanisms between biological ageing and periodontitis. Additionally, two-step MR mediation analysis explored the risk factors in biological ageing-mediated periodontitis.

Results

We analysed data from 3189 participants in the NHANES data and found that higher biological age was associated with increased risk of periodontitis. MR analyses revealed causal associations between biological age measures and periodontitis risk. Frailty (odds ratio [OR] = 2.08, 95% confidence interval [CI]: 1.04–4.18, p = .039) and GrimAge acceleration (OR = 1.16, 95% CI: 1.01–1.32, p = .033) were causally associated with periodontitis risk, and these results were validated in a large-scale meta-periodontitis GWAS dataset. Additionally, the risk effects of body mass index, waist circumference and lifetime smoking on periodontitis were partially mediated by frailty and GrimAge acceleration.

Conclusions

Evidence from cross-sectional survey and MR analysis suggests that biological ageing increases the risk of periodontitis. Additionally, improving the associated risk factors can help prevent both ageing and periodontitis.

目的：研究生物老化与牙周炎之间的关系和潜在因果关系：我们获得了美国国家健康与营养调查（NHANES）和全基因组关联研究（GWAS）的汇总统计数据以及单细胞测序数据。我们采用了基于横截面数据的多元回归分析、孟德尔随机化（MR）和多组学整合分析来探讨生物老化与牙周炎之间的因果关系和潜在的分子机制。此外，两步MR中介分析还探讨了生物老化介导的牙周炎的风险因素：我们分析了 3189 名 NHANES 数据参与者的数据，发现生理年龄越高，患牙周炎的风险越高。磁共振分析显示，生理年龄指标与牙周炎风险之间存在因果关系。虚弱（比值比 [OR] = 2.08，95% 置信区间 [CI]：1.04-4.18，p = .039）和 GrimAge 加速（比值比 [OR] = 1.16，95% 置信区间 [CI]：1.01-1.32，p = .033）与牙周炎风险存在因果关系，这些结果在大规模元牙周炎 GWAS 数据集中得到了验证。此外，体质指数、腰围和终生吸烟对牙周炎的风险影响部分由虚弱和GrimAge加速度介导：来自横断面调查和磁共振分析的证据表明，生物老化会增加牙周炎的风险。此外，改善相关风险因素有助于预防老化和牙周炎。

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引用次数: 0

Supportive periodontal care with or without subgingival instrumentation: Microbiological results of a 2-year randomized clinical trial 使用或不使用龈下器械的支持性牙周护理：为期两年的随机临床试验的微生物学结果。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-02 DOI: 10.1111/jcpe.14038

P. D. M. Angst, U. Van der Velden, C. Susin, S. C. Gomes

Aim

To compare the subgingival microbiota of patients receiving supportive periodontal care (SPC) with and without subgingival instrumentation, over 2 years.

Materials and Methods

This study was a randomized clinical trial that included 62 participants (50.97 ± 9.26 years old; 40 females) who completed non-surgical periodontal therapy. Participants were randomly assigned to receive oral prophylaxis with oral hygiene instructions alone (test) or in combination with subgingival instrumentation (control) during SPC. Pooled subgingival biofilm samples were obtained from four sites per patient at SPC baseline and at 3, 6, 12, 18, and 24 months. Real-time polymerase chain reaction was used for absolute quantification of Eubacteria and the target bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Data were analysed using generalized estimating equations, taking into consideration the clustering of observations within individuals.

Results

No significant differences were found between the experimental groups regarding the mean counts of Eubacteria and target bacteria, as well as the periodontal parameters at the sampled sites. Although significant variability in bacterial counts was present during SPC, all counts after 2 years were not statistically different from those at baseline. Bacterial counts were associated with the presence of plaque, bleeding on probing, mean probing depth ≥3 mm, and follow-up period.

Conclusions

SPC with or without subgingival instrumentation can result in comparable subgingival microbiological outcomes. Clinical trial registration: clinicaltrials.gov: NCT01598155 (https://clinicaltrials.gov/study/NCT01598155?intr=supragingival%20control&rank=4#study-record-dates).

目的：比较接受支持性牙周护理（SPC）和不接受龈下器械治疗的患者在两年内的龈下微生物群：本研究是一项随机临床试验，包括 62 名完成非手术牙周治疗的参与者（50.97 ± 9.26 岁；40 名女性）。参与者被随机分配到在 SPC 期间单独接受口腔卫生指导的口腔预防治疗（试验）或结合龈下器械治疗的口腔预防治疗（对照）。在 SPC 基线期、3、6、12、18 和 24 个月时，从每位患者的四个部位采集龈下生物膜样本。实时聚合酶链反应用于绝对定量龈上真菌和目标细菌牙龈卟啉单胞菌、连翘单胞菌和牙髓震颤素。数据采用广义估计方程进行分析，并考虑了个体内观察结果的聚类：结果：在取样部位，实验组与实验组之间在抑菌和目标细菌的平均数量以及牙周参数方面没有发现明显差异。虽然在 SPC 期间细菌数量存在明显差异，但两年后的所有细菌数量与基线时的细菌数量在统计学上没有差异。细菌计数与牙菌斑的存在、探诊出血、平均探诊深度≥3毫米和随访时间有关：临床试验注册：clinicaltrials.gov：临床试验注册：clinicaltrials.gov：NCT01598155 (https://clinicaltrials.gov/study/NCT01598155?intr=supragingival%20control&rank=4#study-record-dates)。

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引用次数: 0

Efficacy of communicating periodontal risk on psychological outcomes and supragingival plaque control in patients undergoing first periodontal consultation: A parallel-arm, randomized trial 传达牙周风险对首次接受牙周咨询的患者的心理结果和龈上菌斑控制的效果：平行臂随机试验

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-02 DOI: 10.1111/jcpe.14032

Roberto Farina, Anna Simonelli, Maria Elena Guarnelli, Giacomo Secchiati, Giulia Montemezzo, Chiara Scapoli, Leonardo Trombelli

Aim

To evaluate the efficacy of the PerioRisk prognostic tool in implementing the effect of motivational interviewing (MI) on psychological outcomes and supragingival plaque control.

Materials and Methods

Participants underwent MI immediately after their first periodontal visit. According to a parallel-arm, randomized study design, MI was performed with (RISK group) or without (CTR group) information on PerioRisk level and treatment goals based on PerioRisk output. Psychological outcomes were assessed using the Positive Affect Negative Affect Scale (PANAS) and Protection Motivation Theory (PMT). Plaque index (PI) was re-evaluated after 8–12 weeks.

Results

Significant improvements in PMT overall score and PI were observed in CTR and RISK groups, without inter-group difference in PANAS and PMT overall scores and PI. A sub-analysis showed that the overall PMT scores recorded immediately after MI in both CTR and RISK groups for subjects with no tooth loss due to periodontitis were higher than those recorded before MI in subjects with tooth loss due to periodontitis.

Conclusions

At first periodontal visit, MI (implemented with without PerioRisk) has tangible effects on psychological outcomes and supragingival plaque control and seems to anticipate the awareness that is commonly generated by periodontitis-related tooth loss (ClinicalTrials.gov protocol registration ID: NCT05078411).

目的：评估PerioRisk预后工具在实施动机访谈（MI）对心理结果和龈上牙菌斑控制效果方面的有效性：参与者在首次牙周就诊后立即进行动机访谈。根据平行臂随机研究设计，在提供（RISK 组）或不提供（CTR 组）PerioRisk 等级信息和基于 PerioRisk 输出的治疗目标的情况下进行 MI。心理结果采用积极情感消极情感量表（PANAS）和保护动机理论（PMT）进行评估。斑块指数（PI）在 8-12 周后重新评估：结果：CTR 组和 RISK 组的 PMT 总分和 PI 均有显著改善，但 PANAS 和 PMT 总分和 PI 在组间无差异。一项子分析显示，在CTR组和RISK组中，牙周炎导致牙齿缺失的受试者在MI后立即记录的PMT总分高于牙周炎导致牙齿缺失的受试者在MI前记录的总分：在首次牙周就诊时，MI（在不使用 PerioRisk 的情况下实施）对心理结果和龈上牙菌斑控制有明显的效果，而且似乎可以预见牙周炎相关牙齿缺失通常会引起的意识（ClinicalTrials.gov 协议注册编号：NCT05078411）。

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引用次数: 0

Associations between oral health and depression and anxiety: A cross-sectional and prospective cohort study from the UK Biobank 口腔健康与抑郁和焦虑之间的关系：来自英国生物库的一项横断面和前瞻性队列研究。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-01 DOI: 10.1111/jcpe.14039

Jingjing Wang, Yani Wang, Huihui Li, Weijing Wang, Dongfeng Zhang

Aim

To investigate the associations between oral health and depression, anxiety and their comorbidity in the UK Biobank cohort.

Materials and Methods

Oral health problems were self-reported at baseline. Symptoms of depression and anxiety were assessed using the Mental Health Questionnaire (PHQ-4) in a cross-sectional study. In the cohort study, diagnoses of depression and anxiety disorders were based on hospital records. Logistic regression and Cox regression models were used to analyse the association between oral health and depression/anxiety.

Results

A total of 305,188 participants were included in the cross-sectional study, and multivariate analysis showed that periodontal disease was associated with depression and/or anxiety (odds ratio [OR]: 1.79, 95% confidence interval [CI]: 1.73–1.86). In the prospective cohort study involving 264,706 participants, periodontal disease was significantly associated with an increased risk of depression and/or anxiety (hazard ratio [HR]: 1.14, 95% CI: 1.10–1.19), depression (HR: 1.19, 95% CI: 1.13–1.25) and anxiety (HR: 1.13, 95% CI: 1.07–1.19). Periodontal disease was also significantly associated with comorbid depression and anxiety (HR: 1.27, 95% CI: 1.16–1.38). Multiple mediation analysis using baseline inflammatory factors showed that white blood cell count and C-reactive protein explained 3.07% and 3.15% of the association between periodontal disease and depression and anxiety, respectively. However, the results of longitudinal multiple mediation analysis of inflammatory factors at first follow-up (N = 10,673) were not significant.

Conclusions

Periodontal disease was found to be consistently associated with an increased risk of depression, anxiety and their comorbidity.

目的：调查英国生物库队列中口腔健康与抑郁、焦虑及其合并症之间的关系：基线时自我报告口腔健康问题。在横断面研究中，使用心理健康问卷（PHQ-4）评估抑郁和焦虑症状。在队列研究中，抑郁症和焦虑症的诊断以医院记录为依据。研究采用逻辑回归和考克斯回归模型分析口腔健康与抑郁/焦虑之间的关系：多变量分析表明，牙周病与抑郁和/或焦虑相关（几率比 [OR]：1.79，95% 置信区间 [CI]：1.73-1.86）。在涉及 264 706 名参与者的前瞻性队列研究中，牙周病与抑郁和/或焦虑（危险比 [HR]：1.14，95% 置信区间 [CI]：1.10-1.19）、抑郁（HR：1.19，95% 置信区间 [CI]：1.13-1.25）和焦虑（HR：1.13，95% 置信区间 [CI]：1.07-1.19）的风险增加显著相关。牙周病与合并抑郁和焦虑也有明显关联（HR：1.27，95% CI：1.16-1.38）。利用基线炎症因子进行的多重中介分析表明，白细胞计数和 C 反应蛋白分别解释了牙周病与抑郁和焦虑之间 3.07% 和 3.15% 的关联。然而，对首次随访时的炎症因子（N = 10 673）进行纵向多重中介分析的结果并不显著：结论：研究发现，牙周病与抑郁症、焦虑症及其并发症的风险增加一直存在关联。

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引用次数: 0

High-yield nanovesicles extruded from dental follicle stem cells promote the regeneration of periodontal tissues as an alternative of exosomes 牙泡干细胞挤出的高产纳米颗粒作为外泌体的替代物促进了牙周组织的再生。

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-07-01 DOI: 10.1111/jcpe.14036

Lu Liang, Limeiting Wang, Zhenhui Liao, Liya Ma, Pinwen Wang, Junjie Zhao, Jinyan Wu, Hefeng Yang

Aim

To identify an optimized strategy for the large-scale production of nanovesicles (NVs) that preserve the biological properties of exosomes (EXOs) for use in periodontal regeneration.

Materials and Methods

NVs from dental follicle stem cells (DFSCs) were prepared through extrusion, and EXOs from DFSCs were isolated. The yield of both extruded NVs (eNVs) and EXOs were quantified through protein concentration and particle number analyses. Their pro-migration, pro-proliferation and pro-osteogenesis capacities were compared subsequently in vitro. Additionally, proteomics analysis was conducted. To further evaluate the periodontal regeneration potential of eNVs and EXOs, they were incorporated into collagen sponges and transplanted into periodontal defects in rats. In vivo imaging and H&E staining were utilized to verify their biodistribution and safety. Micro-Computed Tomography analysis and histological staining were performed to examine the regeneration of periodontal tissues.

Results

The yield of eNVs was nearly 40 times higher than that of EXOs. Interestingly, in vitro experiments indicated that the pro-migration and pro-proliferation abilities of eNVs were superior, and the pro-osteogenesis potential was comparable to EXOs. More importantly, eNVs exhibited periodontal regenerative potential similar to that of EXOs.

Conclusions

Extrusion has proven to be an efficient method for generating numerous eNVs with the potential to replace EXOs in periodontal regeneration.

目的：确定大规模生产保持外泌体（EXOs）生物特性的纳米颗粒（NVs）的优化策略，以用于牙周再生：材料：通过挤压法制备来自牙泡干细胞（DFSCs）的NVs，并从DFSCs中分离出EXOs。通过蛋白质浓度和颗粒数分析，对挤压NVs（eNVs）和EXOs的产量进行了量化。随后在体外比较了它们的促迁移、促增殖和促骨质生成能力。此外，还进行了蛋白质组学分析。为了进一步评估 eNVs 和 EXOs 的牙周再生潜力，将它们纳入胶原海绵并移植到大鼠的牙周缺损处。利用体内成像和 H&E 染色来验证它们的生物分布和安全性。显微计算机断层扫描分析和组织学染色检查了牙周组织的再生情况：结果：eNVs 的产量比 EXOs 高近 40 倍。有趣的是，体外实验表明，eNVs 的促迁移和促增殖能力更强，其促成骨潜力与 EXOs 相当。更重要的是，eNVs 表现出与 EXOs 相似的牙周再生潜力：结论：事实证明，挤压是生成大量 eNVs 的有效方法，具有取代 EXOs 进行牙周再生的潜力。

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引用次数: 0

Interrupted time series analysis of chronic periodontitis-related procedures before and after the scaling reimbursement policy in Korea 韩国实施洗牙报销政策前后慢性牙周炎相关手术的间断时间序列分析

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology

Pub Date : 2024-06-22 DOI: 10.1111/jcpe.14031

Yu-Rin Kim, Seon-Rye Kim, Minkook Son

Aim

To study the use of a quasi-experimental design to assess the effects of scaling reimbursement policies on the incidence of chronic-periodontitis procedures.

Materials and Methods

Interrupted time series analysis was used to compare the effects before and after policy implementation using data on the number of periodontitis-related procedures from the Korean National Health Insurance Service-National Sample Cohort (n = 740,467) and the Health Screening Cohort (n = 337,904). Periodontitis-related procedures with diagnosis codes were categorized into basic (scaling or root planing), intermediate (subgingival curettage) and advanced (tooth extraction, periodontal flap surgery, bone grafting for alveolar bone defects or guided tissue regeneration). Subjects' demographics and comorbidities were considered. The incidence rate of immediate changes and gradual effects before and after policy implementation was assessed.

Results

Following the policy implementation from July 2013, an immediate increase was observed in total and basic procedures. No significant changes were noted in intermediate and advanced procedures initially. A decrease in the slope of intermediate procedures was observed in both databases. Advanced procedures showed varied trends, with no change in the National Sample Cohort but an increase in the Health Screening Cohort, particularly among subjects with comorbidities.

Conclusions

Following the new policy implementation, the number of intermediate procedures decreased while the number of advanced procedures increased, especially among patients with comorbidities. These findings offer valuable insights on policy evaluation.

材料与方法利用韩国国民健康保险服务-全国抽样队列（n = 740,467 人）和健康检查队列（n = 337,904 人）的牙周炎相关手术数量数据，采用间断时间序列分析法比较政策实施前后的效果。带有诊断代码的牙周炎相关手术分为基础（洗牙或根面平整）、中级（龈下刮治）和高级（拔牙、牙周瓣手术、牙槽骨缺损植骨或引导组织再生）。研究还考虑了受试者的人口统计学特征和合并症。结果2013年7月政策实施后，观察到总手术量和基本手术量立即增加。中级和高级程序最初没有明显变化。在两个数据库中均观察到中级程序的斜率有所下降。结论新政策实施后，中级手术数量减少，而高级手术数量增加，尤其是在有合并症的患者中。这些发现为政策评估提供了宝贵的启示。

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引用次数: 0