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In-situ digestion and micro-destructive sampling for dental proteomic sex determination analysis using polyvinylalcohol-based gel 用聚乙烯醇凝胶进行牙齿蛋白质组性测定的原位消化和微破坏取样
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-31 DOI: 10.1016/j.culher.2026.01.012
Enrico Greco , Matteo Battistella , Elia Marin , Andrea Casini , Leonardo Bellandi , Caterina Gabriele , Alessandro Miani , Prisco Piscitelli , Cătălin A. Lazăr , Pierluigi Barbieri , Rodorico Giorgi , Marco Gaspari

Background

Determining biological sex is pivotal for archaeological and forensic studies, providing insights into past societies, burial practices, and population demographics. Traditional methods, such as morphological analysis and DNA-based techniques, face limitations, including inconclusive results for juveniles, contamination, and genetic material degradation. Additionally, these approaches often involve significant destruction of artifacts, raising ethical concerns. Dental proteomics has emerged as a robust alternative, utilizing the stability of enamel-bound proteins, such as amelogenin isoforms (AMELx and AMELy). However, current sampling techniques are invasive, requiring substantial material removal and risking the integrity of culturally significant specimens.

Results

This study introduces a novel, minimally invasive technique for enamel protein sampling using a polyvinyl alcohol (PVA)-based highly viscous polymeric dispersion (HVPD). Acting as an elastic gel-like system, HVPD enables in-situ digestion and efficient extraction of proteins without compromising the tooth's structural integrity. The method was successfully applied to contemporary and archaeological samples, demonstrating effective recovery of amelogenin isoforms, reduced contamination risk, and compatibility with degraded specimens. This minimally invasive approach achieves reliable detection of all key amelogenin markers required for sex determination, matching the analytical outcome of conventional destructive protocols while preserving specimen integrity.’

Significance

This methodology offers transformative potential for archaeology, anthropology, and forensic science, bridging scientific innovation with ethical stewardship. By preserving the physical and cultural integrity of artifacts, it addresses a critical need for sustainable research practices. Reliable biological sex determination in challenging contexts expands the scope of proteomic applications, providing new insights into past societies while safeguarding irreplaceable heritage for future generations.
生物性别的确定对于考古和法医研究至关重要,它可以提供对过去社会、埋葬习俗和人口统计的见解。传统的方法,如形态分析和基于dna的技术,面临着局限性,包括对幼鱼的不确定结果、污染和遗传物质降解。此外,这些方法通常涉及对人工制品的重大破坏,引起道德问题。牙齿蛋白质组学已经成为一种强有力的替代方法,它利用了釉质结合蛋白的稳定性,如釉质原蛋白异构体(AMELx和AMELy)。然而,目前的采样技术是侵入性的,需要大量的材料去除,并冒着具有文化意义的标本完整性的风险。本研究介绍了一种新颖的、微创的珐琅蛋白取样技术,该技术采用聚乙烯醇(PVA)为基础的高粘性聚合物分散体(HVPD)。作为一种弹性凝胶状系统,HVPD可以在不损害牙齿结构完整性的情况下原位消化和有效提取蛋白质。该方法已成功应用于当代和考古样品,证明了淀粉原蛋白同种异型的有效恢复,降低了污染风险,并且与降解样品具有相容性。这种微创方法可以可靠地检测出性别鉴定所需的所有关键淀粉原蛋白标记,与传统破坏性方法的分析结果相匹配,同时保持标本的完整性。这种方法为考古学、人类学和法医学提供了变革的潜力,将科学创新与道德管理联系起来。通过保护文物的物理和文化完整性,它解决了可持续研究实践的关键需求。在具有挑战性的环境中可靠的生物性别确定扩展了蛋白质组学应用的范围,为过去的社会提供了新的见解,同时为后代保护了不可替代的遗产。
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引用次数: 0
Adhesives for cultural heritage conservation: Functions, performance evaluation, and application development 文化遗产保护用胶粘剂:功能、性能评价及应用开发
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-31 DOI: 10.1016/j.culher.2026.01.010
Jiaojiao Liu , Zhi Luo , Jianghua Wei , Yi Li , Junchang Yang , Ran Chen
Adhesives play a vital role in the restoration, reinforcement, and stabilization of cultural heritage, yet their use is challenged by inherent trade-offs: performance versus substrate compatibility, bonding strength versus reversibility, and durability versus environmental stability. These conflicts complicate the pursuit of both scientific rigor and sustainability in conservation. This review surveys adhesive applications across diverse heritage substrates—including stone, wood, painted artifacts, ceramics, textiles, metals, and paper—with emphasis on bonding mechanisms and key performance criteria. It reassesses traditional materials such as glutinous rice mortar and animal glue through modern analytical perspectives, and evaluates the advancement and limitations of synthetic polymers, notably acrylic and epoxy resins. Emerging technologies are examined, including nanocomposite systems for enhanced compatibility and mechanical integrity, bio-based and biomimetic adhesives for green conservation, and smart or self-healing materials addressing reversibility challenges. Recent progress in analytical techniques for characterizing ancient adhesives and understanding environmental degradation mechanisms is also reviewed. The paper concludes by identifying critical challenges and future directions, underscoring interdisciplinary innovation and sustainability as essential drivers for next-generation adhesive development aligned with conservation ethics. This framework aims to guide research toward materials that combine high performance, compatibility, and ethical compliance.
粘合剂在文化遗产的修复、加固和稳定中发挥着至关重要的作用,但它们的使用受到固有权衡的挑战:性能与基材兼容性、粘合强度与可逆性、耐久性与环境稳定性。这些冲突使追求科学的严谨性和保护的可持续性变得复杂。本文综述了粘合剂在各种遗产基材上的应用,包括石头、木材、彩绘文物、陶瓷、纺织品、金属和纸张,重点介绍了粘合机制和关键性能标准。它通过现代分析视角重新评估传统材料,如糯米砂浆和动物胶,并评估合成聚合物的进步和局限性,特别是丙烯酸和环氧树脂。研究了新兴技术,包括增强相容性和机械完整性的纳米复合材料系统,用于绿色保护的生物基和仿生粘合剂,以及解决可逆性挑战的智能或自修复材料。综述了古代胶粘剂表征分析技术和环境降解机制的最新进展。论文最后指出了关键挑战和未来方向,强调跨学科创新和可持续性是下一代粘合剂开发与保护伦理一致的重要驱动力。该框架旨在指导研究结合高性能,兼容性和道德合规的材料。
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引用次数: 0
Non-invasive methods for characterising letterpress prints: Surface microscopy and profilometry 凸版印刷特征的非侵入性方法。表面显微术和轮廓测定法
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-31 DOI: 10.1016/j.culher.2026.01.013
Iris Bautista-Morenilla , Júlia María Olivera López , Cristina Ruiz-Recasens , Oriol Moret Viñals , Lluís Casas Duocastella , Gema Campo-Francés
This study presents a methodological framework for characterising letterpress prints by combining morphological analysis with three-dimensional measurements. Original prints, as well as controlled mock-ups produced in the laboratory were analysed with surface microscopy to assess ink distribution on the paper. Optical profilometry was used to quantify the reverse embossing generated in the paper by the type-form.
Results show that the various methods and materials employed for letterpress printing over time lead to differences regarding ink distribution and relief levels. These are described and presented as key identification features through which the origin of early and contemporary letterpress prints can be determined.
The paper provides visual examples of the traits that letterpress prints can show throughout history and establishes a methodological approach for their characterisation. Therefore, this research expands the range of methodologies available for the technical cataloguing of letterpress prints.
本研究提出了一种方法学框架,通过结合形态分析和三维测量来表征凸版印刷。用表面显微镜对原始印刷品和实验室生产的受控模型进行分析,以评估纸张上的油墨分布。采用光学轮廓法定量分析了由字型在纸张上产生的反向压纹。结果表明,随着时间的推移,凸版印刷采用的各种方法和材料导致油墨分布和浮雕水平的差异。这些被描述和呈现为关键的识别特征,通过这些特征可以确定早期和当代凸版印刷的起源。本文提供了凸版印刷在整个历史中可以显示的特征的视觉例子,并建立了一种方法方法来描述它们的特征。因此,本研究扩大了活版印刷技术编目的方法范围。
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引用次数: 0
Portable ER-FTIR as a non-destructive method to pre-screen collagen for ZooMS analysis in archaeology 便携式ER-FTIR无损筛选考古中ZooMS分析中胶原蛋白的方法
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-24 DOI: 10.1016/j.culher.2026.01.009
M. Di Matteo , K. McGrath , C. Lemorini , S. Nunziante-Cesaro , S. Soncin
In the last decades, archaeology has witnessed a significant increase in the use of biomolecular analyses to study a variety of materials, including skeletal elements, as they are frequently preserved in archaeological deposits and directly linked to cultural and economic dynamics of ancient human populations. Radiocarbon dating, isotopic studies, and proteomic analyses are particularly useful to explore these questions, while their success is highly dependent on the state of preservation of collagen, the most abundant component of the organic fraction of skeletal elements. Over time collagen degrades, and its preservation is often compromised in very ancient archaeological contexts or when taphonomic processes are particularly severe, which can significantly limit the feasibility of subsequent biomolecular analyses.
The aim of this study is to test whether external reflectance Fourier Transform Infrared Spectroscopy (ER-FTIR) can serve as a rapid, non-destructive pre-screening tool for assessing collagen preservation prior to ZooMS analysis. To evaluate the method's effectiveness, various faunal bone fragments were selected from different archaeological contexts (e.g., rock shelters, pits in dune fields, etc.) located in the Central Sahara (SW Libya), dating to the Middle and Late Holocene (8300–3400 cal BP). The bone fragments were first subjected to ER-FTIR analysis and then to ZooMS (Zooarchaeology by Mass Spectrometry) to compare the results and assess the presence of collagen in the samples.
Our results indicate that collagen was detected in about one-third of the samples, consistently associated with specific spectral features and further validated by ZooMS analyses. The method effectively distinguished well-preserved from poorly preserved samples while avoiding destructive sampling. This pre-screening approach reduces time and financial costs and safeguards the integrity of archaeological bones. Beyond its practical application, it also contributes to bioarchaeology and conservation science by providing a reproducible, non-destructive framework for evaluating biomolecular preservation across different sites and periods.
在过去的几十年里,考古学见证了生物分子分析在研究各种材料方面的显著增加,包括骨骼元素,因为它们经常被保存在考古沉积物中,并与古代人类种群的文化和经济动态直接相关。放射性碳测年、同位素研究和蛋白质组学分析对探索这些问题特别有用,而它们的成功高度依赖于胶原蛋白的保存状态,胶原蛋白是骨骼元素有机部分中最丰富的成分。随着时间的推移,胶原蛋白会降解,在非常古老的考古背景下,或者在埋藏过程特别严重的情况下,胶原蛋白的保存往往会受到损害,这极大地限制了随后生物分子分析的可行性。本研究的目的是测试外反射傅里叶变换红外光谱(ER-FTIR)是否可以作为一种快速,非破坏性的预筛选工具,用于评估ZooMS分析之前的胶原保存情况。为了评估该方法的有效性,研究人员从位于中撒哈拉沙漠(利比亚西南部)的不同考古环境(如岩石掩体、沙丘坑等)中选择了各种动物骨碎片,时间可追溯到全新世中晚期(8300-3400 cal BP)。骨碎片首先进行ER-FTIR分析,然后进行ZooMS(动物考古质谱)分析,以比较结果并评估样品中胶原蛋白的存在。我们的结果表明,在大约三分之一的样品中检测到胶原蛋白,与特定的光谱特征一致,并通过ZooMS分析进一步验证。该方法有效地区分了保存良好和保存不良的样品,同时避免了破坏性采样。这种预先筛选的方法减少了时间和经济成本,并保障了考古骨骼的完整性。除了实际应用之外,它还为生物考古学和保护科学提供了一个可复制的、非破坏性的框架,用于评估不同地点和时期的生物分子保护。
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引用次数: 0
Chinese vernacular villages renovation: A methodology for establishing a 3D interactive database 中国乡土村落改造:三维互动数据库的建立方法
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-24 DOI: 10.1016/j.culher.2026.01.004
Dan Hu , Liheng Zhu , Jiajia Wei
Vernacular villages sustainable development has emerged as a crucial component of China’s rural revitalization strategy. How to integrate 3D digital technology with vernacular village renovation has become a topic worthy of in-depth exploration.
This research aims to develop a methodology for constructing a web-based visualization and comprehensive database. It is designed to synthesize traditional renovation methods with 3D digital modeling in service of vernacular village revitalization. Its specific aims include: supplying both authentic and derivative information for renovation; enabling the long-term preservation of cultural heritage; providing visualized renovation strategies; facilitating 3D visual design within an authentic context; and offering an interactive platform for user participation.
Methodologically, it derives the database requirements and content from an analysis of relevant literature and renovation cases. It critically formulates a database design methodology, building upon existing best practices, that is tailored to vernacular villages in China. It has been practiced in Muka Village as a case study, demonstrating its potential applicability to other vernacular villages.
The research resulted in a database structured into a five-level hierarchy: National/Provincial, Regional, Village, Settlement, and Building. Its content encompasses multi-level 3D models and derived data, policy documents, visualized renovation strategies, and interactive model upload/download functionalities. The process is: data collection and processing, derived data generation, data format unification, and visualization.
乡土村落可持续发展已成为中国乡村振兴战略的重要组成部分。如何将三维数字技术与乡土村落改造相结合,成为一个值得深入探索的课题。本研究旨在建立一种基于网络的可视化综合数据库的建构方法。将传统的改造方法与三维数字建模相结合,服务于乡土乡村振兴。其具体目标包括:为改造提供真实的和衍生的信息;使文化遗产得以长期保存;提供可视化的改造策略;在真实的环境中促进3D视觉设计;并提供用户参与的互动平台。在方法上,通过对相关文献和改造案例的分析,得出数据库的需求和内容。它在现有最佳实践的基础上,批判性地制定了一种数据库设计方法,为中国的乡土村庄量身定制。以木卡村为例进行了实践研究,证明了其在其他乡土村落的潜在适用性。研究结果将数据库分为五个层次结构:国家/省、地区、村庄、聚落和建筑。其内容包括多级3D模型和衍生数据、政策文件、可视化改造策略和交互式模型上传/下载功能。该过程是:数据的收集和处理、派生数据的生成、数据格式的统一和可视化。
{"title":"Chinese vernacular villages renovation: A methodology for establishing a 3D interactive database","authors":"Dan Hu ,&nbsp;Liheng Zhu ,&nbsp;Jiajia Wei","doi":"10.1016/j.culher.2026.01.004","DOIUrl":"10.1016/j.culher.2026.01.004","url":null,"abstract":"<div><div>Vernacular villages sustainable development has emerged as a crucial component of China’s rural revitalization strategy. How to integrate 3D digital technology with vernacular village renovation has become a topic worthy of in-depth exploration.</div><div>This research aims to develop a methodology for constructing a web-based visualization and comprehensive database. It is designed to synthesize traditional renovation methods with 3D digital modeling in service of vernacular village revitalization. Its specific aims include: supplying both authentic and derivative information for renovation; enabling the long-term preservation of cultural heritage; providing visualized renovation strategies; facilitating 3D visual design within an authentic context; and offering an interactive platform for user participation.</div><div>Methodologically, it derives the database requirements and content from an analysis of relevant literature and renovation cases. It critically formulates a database design methodology, building upon existing best practices, that is tailored to vernacular villages in China. It has been practiced in Muka Village as a case study, demonstrating its potential applicability to other vernacular villages.</div><div>The research resulted in a database structured into a five-level hierarchy: National/Provincial, Regional, Village, Settlement, and Building. Its content encompasses multi-level 3D models and derived data, policy documents, visualized renovation strategies, and interactive model upload/download functionalities. The process is: data collection and processing, derived data generation, data format unification, and visualization.</div></div>","PeriodicalId":15480,"journal":{"name":"Journal of Cultural Heritage","volume":"78 ","pages":"Pages 67-78"},"PeriodicalIF":3.3,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146075499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shining a light on the degradation of triarylmethane dyes: Multi-analytical study of a faded 1880s printed cotton dress 揭示三芳基甲烷染料的降解:一件褪色的19世纪80年代印花棉裙的多分析研究
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-23 DOI: 10.1016/j.culher.2026.01.008
Catarina M. Pinto , David Buti , Letizia Monico , Aldo Romani , Lucia Burgio , Brenda Doherty
This study examines the fading of the blue-green background in an 1885 printed cotton dress by Edmund Potter & Co., in the collection of the Victoria and Albert Museum (V&A). Uneven fading patterns indicate differential light exposure, with protected areas retaining greater vibrancy. An in-situ analytical campaign employing portable UV–VIS–NIR spectroscopy, colorimetry, and VIS hyperspectral imaging identified dye constituents and evaluated their conservation state. Complementary micro-destructive Surface Enhanced Raman spectroscopy suggests the presence of early synthetic triarylmethane dyes. To further investigate their photo-fading behaviour, mordanted cotton mock-ups dyed with selected blue-green triarylmethane dyes commercially available at the time of the dress’s production, namely, diamond green B (C.I. 42,000, Basic Green 4, 1877), diamond green G (C.I. 42,040, Basic Green 1, 1879) and yellowish light green SF (C.I. 42,095, Acid Green 5, late 19th Century) were subjected to controlled aging and monitored. Comparative analysis with in situ data provided contextual insight into the photochemical behaviour of the dyes, highlighting differences associated with substituent type and orientation and their potential influence on observed photoproducts, including benzophenones. These findings advance the understanding of triarylmethane dye fading in important historical textiles and inform conservation strategies for museum display of such dye sensitive collections.
本研究考察了维多利亚和阿尔伯特博物馆(V&;A)收藏的埃德蒙·波特公司1885年印花棉布连衣裙的蓝绿色背景褪色情况。不均匀的褪色模式表明不同的光照,保护区保留更大的活力。采用便携式紫外-可见-近红外光谱、比色法和可见高光谱成像进行原位分析,鉴定染料成分并评估其保存状态。互补微破坏表面增强拉曼光谱表明存在早期合成的三芳基甲烷染料。为了进一步研究它们的光褪色行为,我们选择了在服装生产时可以买到的蓝绿色三芳基甲烷染料,即钻石绿B (C.I. 42000,基本绿4,1877年)、钻石绿G (C.I. 42,040,基本绿1,1879年)和淡黄浅绿SF (C.I. 42,095,酸性绿5,19世纪末),对棉质模型进行了控制老化和监测。与原位数据的比较分析提供了对染料光化学行为的上下文洞察,突出了与取代基类型和取向相关的差异及其对观察到的光产物(包括二苯甲酮)的潜在影响。这些发现促进了对重要历史纺织品中三芳基甲烷染料褪色的理解,并为博物馆展示此类染料敏感收藏品提供了保护策略。
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引用次数: 0
Black manganese crusts on Galician Romanesque buildings: first analytical data on characterization and cleaning 加利西亚罗马式建筑上的黑锰结壳:关于特征和清洗的第一个分析数据
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-22 DOI: 10.1016/j.culher.2026.01.006
T. Rivas , L. Espiño , S. Pozo-Antonio , C. López
This work presents the results of the characterization of Mn-rich black crusts developed on granite in a Galician Romanesque monastery and the initial experiments aimed at their removal. Once the crusts were characterized from a chemical, mineralogical and micromorphological point of view, several in situ cleaning methods was performed: microabrasion, a Nd:YAG laser and chemical cleaning processes (aqua regia, EDTA, oxalic acid, gallic acid and formic acid solutions). An in situ analytical methodology based on optical microscopy, colour spectrophotometry, LIBS and Raman spectroscopy was applied to characterize the cleaning effectiveness and the side effects exerted in the granite underneath. Among all the methods, saturated oxalic acid proved to be the most effective, although slight calcium contamination was detected. In contrast, less effective methods such as laser cleaning and microabrasion resulted in textural alterations in the granite.
这项工作介绍了加利西亚罗马式修道院花岗岩上富锰黑色外壳的表征结果,以及旨在去除它们的初步实验。一旦从化学、矿物学和微观形态学的角度对结壳进行了表征,就可以采用几种原位清洗方法:微磨损、Nd:YAG激光和化学清洗工艺(王水、EDTA、草酸、没食子酸和甲酸溶液)。采用基于光学显微镜、彩色分光光度法、液相色谱法和拉曼光谱的原位分析方法,表征了对花岗岩的清洗效果和副作用。在所有的方法中,饱和草酸法被证明是最有效的,尽管检测到轻微的钙污染。相比之下,激光清洗和微磨损等效果较差的方法会导致花岗岩的纹理变化。
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引用次数: 0
Dual-format indirect ELISA detection of food residues in late Roman and early medieval ceramics: Integrating antibodies and DNA aptamers for ancient protein and β-D-glucan analysis 罗马晚期和中世纪早期陶瓷中食物残留的双格式间接ELISA检测:整合抗体和DNA适体用于古代蛋白质和β- d -葡聚糖分析
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-22 DOI: 10.1016/j.culher.2026.01.005
Janez Kosel , K. Patrick Fazioli , Andrej Magdič , Zvezdana Modrijan , Jaroslav Pavelka , Polonca Ropret
Food and its associated material culture offer an important window into past social worlds, but difficulties in inferring vessel function from and the degradation of organic residues on pottery can hinder archaeological investigations of culinary practices. To overcome these limitations, we applied indirect ELISA assays using both polyclonal antibodies and synthetic aptamers to detect specific proteins and a cereal polysaccharide in ceramic sherds from two hilltop sites in north-eastern Slovenia: an early medieval settlement beneath Ptuj Castle (7th-9th centuries CE) and a Late Roman–early medieval fortified site at Ančnikovo Gradišče (4–5th, 7–10th centuries CE). Sherds bearing charred cooking residues were powder-extracted and assayed on MaxiSorp microtiter plates for six protein targets (ovalbumin, lysozyme, gluten, casein, collagen, and myosin) and for (1→3)-β-d-glucan target using matching primary antibodies and biotinylated DNA aptamers, with detection achieved by adding alkaline phosphatase–conjugated secondary antibodies to label primary antibody binding or alkaline phosphatase–conjugated streptavidin to label aptamer binding. At Ptuj, both formats identified egg proteins, bone collagen, myosin and gluten on individual sherds, with aptamers consistently yielding larger ΔOD margins than antibodies. At Ančnikovo Gradišče, meat residues dominated: myosin and collagen were detected on most Late Roman sherds, while sherd 402 (early medieval) uniquely registered cereal markers—gliadin and β-d-glucan—alongside meat and egg proteins. Casein appeared in only one Late Roman vessel. Co-occurrence of animal and plant residues implies composite dishes such as stews or porridges. Our findings demonstrate that aptamer‐based ELISAs offer enhanced sensitivity for archaeological proteins, and that protein and polysaccharide residue analysis can reconstruct nuanced dietary patterns. This approach holds promise for broad application in contexts lacking textual records. Caution should be taken when attempting to extrapolate broader patterns from this limited dataset.
食物及其相关的物质文化为了解过去的社会世界提供了一个重要的窗口,但是从陶器上的有机残留物推断容器功能和降解的困难可能会阻碍烹饪实践的考古调查。为了克服这些局限性,我们采用间接ELISA法,使用多克隆抗体和合成适体检测斯洛文尼亚东北部两个山顶遗址的陶瓷碎片中的特定蛋白质和谷物多糖:Ptuj城堡下的中世纪早期定居点(公元7 -9世纪)和anannikovo Gradišče的罗马晚期-中世纪早期防御遗址(公元4 - 5世纪,公元7 - 10世纪)。对烧焦的烹饪残渣进行粉末提取,并在MaxiSorp微滴板上检测6个蛋白靶点(卵清蛋白、溶菌酶、谷蛋白、酪蛋白、胶原蛋白和肌球蛋白)和(1→3)-β-d-葡聚糖靶点,使用匹配的一抗和生物素化DNA适配体,通过添加碱性磷酸酶偶联的二抗标记一抗结合或碱性磷酸酶偶联的链亲和素标记适配体结合来检测。在Ptuj,两种格式都能在单个片段上识别鸡蛋蛋白、骨胶原蛋白、肌球蛋白和谷蛋白,适配体的ΔOD边缘始终比抗体大。在an尼科沃Gradišče,肉类残留物占主导地位:在大多数晚期罗马碎片上检测到肌球蛋白和胶原蛋白,而碎片402(中世纪早期)独特注册的谷物标记-麦胶蛋白和β-d-葡聚糖-以及肉类和鸡蛋蛋白质。酪蛋白只出现在一艘晚期罗马船只上。同时出现的动物和植物残留物意味着混合菜肴,如炖菜或粥。我们的研究结果表明,基于适体的elisa对考古蛋白质具有更高的敏感性,蛋白质和多糖残留分析可以重建细微的饮食模式。这种方法有望在缺乏文本记录的环境中得到广泛应用。当试图从这个有限的数据集推断更广泛的模式时,应该谨慎。
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引用次数: 0
In situ characterization of nephrite beads from the Hongshan culture: Unveiling techniques, materials and sources 红山文化中软玉珠的原位表征:揭示技术、材料和来源
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-21 DOI: 10.1016/j.culher.2026.01.007
Dian Chen , Guoxiang Liu , Yuanqiu Li , Jiangtao Liu , Tingting Shi , Manda Bai , Yuanchi Shang , Wugan Luo
The Hongshan Culture, a Neolithic society in northeastern China, is renowned for its sophisticated jade artifacts. While past research has focused mainly on large or ceremonial jade pieces, smaller artifacts like beads have received limited systematic study. This research examines 68 nephrite beads from the Nasitai site in Inner Mongolia using in-situ, non-destructive analytical techniques, including microscopic observation, FTIR, Raman spectroscopy, pXRF, and multispectral imaging, to explore craftsmanship, material properties, and provenance. The spiral-grooved notches suggest a unique Hongshan cutting technique involving hole-drilling and the use of a sand-coated rope to split the jade. Ox-nostril-shaped drill marks indicate primitive perforation methods for stringing. Spectral analysis confirmed that the beads are made of tremolite, showing minimal weathering and excellent preservation. Elemental profiling highlighted consistent Ca/Ba ratios, while distinct Mn/Fe and Ni patterns suggest the potential for distinguishing nephrite sources. This analysis indicates that the jade beads may originate from Xiuyan nephrite. Additionally, multispectral imaging further supports this provenance conclusion. This study broadens understanding of Hongshan jade sourcing and technology, demonstrating the value of non-invasive methods in artifact analysis. The findings contribute to discussions on early Chinese jade trade and craft networks, setting a foundation for future comparative studies on Neolithic jade traditions.
红山文化是中国东北部的一个新石器社会,以其精致的玉器而闻名。虽然过去的研究主要集中在大型或仪式玉器上,但像珠子这样较小的文物得到了有限的系统研究。本研究使用原位无损分析技术,包括显微观察、FTIR、拉曼光谱、pXRF和多光谱成像,对内蒙古纳西泰遗址的68颗软玉珠进行了研究,以探索工艺、材料特性和来源。螺旋形的凹槽表明,这是一种独特的红山切割技术,包括钻孔和使用涂砂绳来切割玉石。牛鼻孔形状的钻痕表明原始的射孔管柱方法。光谱分析证实,这些珠子是由透闪石制成的,风化作用最小,保存完好。元素谱分析强调了一致的Ca/Ba比率,而不同的Mn/Fe和Ni模式表明了区分软玉来源的潜力。分析表明,该玉珠可能来源于岫岩软玉。此外,多光谱成像进一步支持了这一结论。本研究拓宽了对红山玉石来源和工艺的认识,展示了非侵入性方法在人工制品分析中的价值。这些发现有助于讨论早期中国玉器贸易和工艺网络,为未来对新石器时代玉器传统的比较研究奠定基础。
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引用次数: 0
Biogeophysical impact of algal biofilms developed on granite and mortar substrates under night-time ornamental lighting 夜间观赏照明下花岗岩和砂浆基质上藻类生物膜的生物地球物理影响
IF 3.3 2区 综合性期刊 0 ARCHAEOLOGY Pub Date : 2026-01-21 DOI: 10.1016/j.culher.2026.01.002
Anxo Méndez , Davide Gulotta , David M. Freire-Lista , Patricia Sanmartín
<div><div>Ornamental LED lighting is commonly used to illuminate architectural heritage at night. Since phototrophs rely on light as an energy source through photosynthesis, the artificial extension of the natural photoperiod can impact microalgae and cyanobacteria dwelling on stone surfaces. These organisms form subaerial biofilms (SABs), which lead to biofouling of building materials and pose challenges for heritage conservation. For the first time, the present study looked in depth at what happens at the SAB-substrate interface when biofilm formation occurs under daylight followed by ornamental lighting. Granite (the main material in historical buildings and structures in NW Spain and many other regions of the world) and cement (the most widely used material for construction, especially in modern and contemporary architecture) have been used as test substrates, inhabited by algal biofilms mainly composed by the green algae <em>Bracteacoccus minor</em> and <em>Stichococcus bacillaris</em>. Tests were conducted after a 3-month exposure to different ornamental LED light conditions. The tested conditions comprised a photoperiod of 13 h of daylight, followed by 6 h of exposure to different ornamental LED illumination, and 5 h of darkness (imitating the photoperiod of ornamentally illuminated monuments). The ornamental LED lighting conditions tested were an innovative amber+green light (at 3000 K, with biostatic effect and under trial), two lights suitable for illuminating the urban fabric: warm white light (at 2580 K, with a yellower hue) and cool white light (at 4600 K, with bluer hue), and a reference scenario without ornamental lighting (i.e., darkness). Blank specimens without colonization were also included. Surface roughness and hardness, VIS-light spectrophotometry, static contact angle and water absorption, as well as petrographic and 3D microscopy, were used to evaluate the lighting impact on the SABs interactions with the building material substrates. The presence of SAB increased the surface hardness, water absorption time and static contact angle while reducing the surface hardness of both the granite and mortar specimens. The SAB-substrate interface exposed to amber+green light behaved similarly to the biofouled specimens without ornamental illumination, minimizing its effects in comparison to both white lights tested, which further affected those parameters. The color differences of the surface, changed by the ornamental lighting conditions, were also minimized under the amber+green light. The results suggest a lower level of SAB development under the innovative amber+green light. Petrographic microscopy of thin sections reveals differential SAB coverage across the granite surface, depending on local mineralogical differences, with biotite appearing uncolonized. In the mortar specimens, the SAB was thinner and appeared to fill the macropores. The results can inform the design of more effective ornamental lighting strategies in cities,
装饰性LED照明通常用于在夜间照亮建筑遗产。由于光养生物依靠光作为光合作用的能量来源,人工延长自然光周期会影响居住在石头表面的微藻和蓝藻。这些生物形成水下生物膜(SABs),导致建筑材料的生物污染,给文物保护带来挑战。本研究首次深入研究了当生物膜在日光和观赏照明下形成时,在sabb -基质界面发生了什么。花岗岩(西班牙西北部和世界许多其他地区历史建筑和结构的主要材料)和水泥(最广泛使用的建筑材料,特别是在现代和当代建筑中)已被用作试验基质,藻类生物膜主要由绿藻小苞球菌和杆菌性囊球菌组成。在不同的装饰性LED光条件下暴露3个月后进行测试。测试条件包括13小时的日光,随后6小时暴露在不同的装饰性LED照明下,以及5小时的黑暗(模仿装饰性照明纪念碑的光周期)。测试的装饰性LED照明条件是一种创新的琥珀色+绿光(3000 K,具有生物静力效应,正在试验中),两种适合照亮城市结构的灯:暖白光(2580 K,带有黄色色调)和冷白光(4600 K,带有蓝色色调),以及一个没有装饰性照明的参考场景(即黑暗)。未定植的空白标本也包括在内。利用表面粗糙度和硬度、可见光分光光度法、静态接触角和吸水率以及岩石学和3D显微镜来评估光照对SABs与建筑材料基板相互作用的影响。SAB的存在增加了花岗岩和砂浆试件的表面硬度、吸水时间和静接触角,降低了花岗岩和砂浆试件的表面硬度。暴露在琥珀色+绿光下的ab -基质界面与没有装饰照明的生物污染样品的表现相似,与测试的两种白光相比,其影响最小,这进一步影响了这些参数。在琥珀色+绿光下,由于观赏性照明条件的改变,表面的色差也被最小化。结果表明,琥珀色+绿光下SAB的发展水平较低。薄片岩相显微镜显示花岗岩表面SAB覆盖的差异,取决于当地矿物学差异,黑云母似乎未定植。在砂浆试样中,SAB更薄,似乎填充了大孔隙。研究结果可以为城市中更有效的观赏照明策略的设计提供信息,通过使用琥珀色+绿光来减少生物殖民化。
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Journal of Cultural Heritage
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