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Novel regimens of phytopolyphenols and celecoxib enhancing efficacy and selectivity of anticancer effects of chemotherapeutic agents on cultured cancer cells 植物多酚和塞来昔布的新方案可提高化疗药物对培养癌细胞的抗癌效果和选择性
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.02.013
Ming-Yung Chou , Shoei-Yn Lin-Shiau

Background/purpose

Explorations of novel regimens enhancing efficacy and selectivity of chemotherapeutic agents are urgent to solve the problems of cancer therapy. This study aimed to explore synergistic anticancer effects of novel regimens of phytopolyphenols [curcumin (C), tea polyphenols (G) or GC] with celecoxib (Cl) and ZnSO4.

Materials and methods

Antiproliferative effects of drugs on cultured cancer cells and pathogenic biofilms were assayed by MTT and optical density (OD600) respectively; their inhibition on efflux pump (Na+-K+-ATPase) was measured by colorimetric methods. Synergistic (CI < 1) anticancer effects were evaluated by the equations of combination index (CI) and efficacy index (EI).

Results

Both Cl and methotrexate (MTX) alone exhibited inhibitory effects not only on proliferation and efflux pump of cultured cancer cells but also pathogenic biofilm formation. Phytopolyphenols (P) and MTX potentiated these inhibitory effects of Cl. In addition, novel regimens containing Cl, memantine (Mem) or thioridazine (TRZ) further enhanced not only efficacy and selectivity of anticancer effects but also inhibition on efflux pump and pathogenic biofilm formation of four chemotherapeutic agents (MTX, cisplatin, 5-fluorouracil and doxorubicin) respectively.

Conclusion

In this study, novel regimens of phytopolyphenols (P), targeting drugs (T; Cl, Mem or TRZ) and metal ions (M; ZnSO4) so called PTM regimens exerted not only by themselves but also markedly potentiated efficacy and selectivity of anticancer effects of four chemotherapeutic agents. Because of their potent inhibitions on efflux pump and pathogenic biofilm formation, these combinatorial novel regimens were expected to be able to overcome the problems of multidrug resistant cancers and merit for further clinical studies.

探索提高化疗药物疗效和选择性的新型方案是解决癌症治疗问题的当务之急。本研究旨在探索植物多酚[姜黄素(C)、茶多酚(G)或 GC]与塞来昔布(Cl)和硫酸锌的协同抗癌效果。药物对培养的癌细胞和病原生物膜的抗增殖作用分别通过 MTT 和光密度(OD600)进行检测;对外流泵(Na-K-ATPase)的抑制作用则通过比色法进行检测。协同(CI < 1)抗癌效果通过组合指数(CI)和药效指数(EI)方程进行评估。Cl 和单独的甲氨蝶呤(MTX)不仅对培养癌细胞的增殖和外排泵有抑制作用,而且对致病生物膜的形成也有抑制作用。植物多酚(P)和 MTX 增强了 Cl 的这些抑制作用。此外,含有 Cl、美金刚(Mem)或硫利达嗪(TRZ)的新方案不仅进一步增强了抗癌效果和选择性,还分别增强了对四种化疗药物(MTX、顺铂、5-氟尿嘧啶和多柔比星)的外排泵和致病性生物膜形成的抑制作用。在这项研究中,由植物多酚(P)、靶向药物(T;Cl、Mem 或 TRZ)和金属离子(M;ZnSO)组成的新型方案(即 PTM 方案)不仅本身具有抑制作用,而且还显著增强了四种化疗药物的抗癌功效和选择性。由于这些组合新方案能有效抑制外排泵和病原体生物膜的形成,因此有望克服多药耐药性癌症的问题,值得进一步临床研究。
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引用次数: 0
The effect of low-level laser therapy on osteoclast differentiation: Clinical implications for tooth movement and bone density 低强度激光疗法对破骨细胞分化的影响:对牙齿移动和骨密度的临床影响
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.03.023
Chun-Yi Huang , Huynh Hoai Thuong Le , Hsiao-Chi Tsai , Chih-Hsin Tang , Jian-Hong Yu

Background/purpose

Osteoclast differentiation is crucial for orchestrating both tooth movement and the maintenance of bone density. Therefore, the current study sought to explore the impact of low-level laser therapy (LLLT) on osteoclast differentiation, functional gene expression, molecular signaling pathways, and orthodontic tooth movement in clinical settings.

Materials and methods

The RAW 264.7 cell line served as the precursor for osteoclasts, and these cells underwent irradiation using a 808-nm LLLT. Osteoclast differentiation was assessed through tartrate-resistant acid phosphatase (TRAP) staining. Functional gene expression levels were evaluated using real-time quantitative polymerase chain reaction (RT-qPCR) while signaling molecules were examined through Western blot analysis. In the clinical study, 12 participants were enrolled. Their tooth movement was monitored using a TRIOS desktop scanner. Bone density measurements were conducted using Mimics software, which processed cone-beam computed tomography (CBCT) images exported in Digital Imaging and Communications in Medicine (DICOM) format.

Results

We found that LLLT effectively promoted receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation and the expression of osteoclast functional genes, including matrix metallopeptidase 9 (MMP9), nuclear factor of activated T-cells cytoplasmic 1(NFATc1), tartrate-resistant acid phosphatase (TRAP) and cathepsin K (CTSK) in RAW264.7 cells. Clinically, the cumulative tooth movement over 90 days was significantly higher in the laser group than in the control group.

Conclusion

Our research demonstrates that LLLT not only significantly promotes osteoclast differentiation but is also a valuable adjunct in orthodontic therapy.

背景/目的破骨细胞的分化对于协调牙齿移动和维持骨密度至关重要。因此,本研究试图探讨低强度激光疗法(LLLT)对破骨细胞分化、功能基因表达、分子信号通路和临床正畸牙齿移动的影响。材料与方法 RAW 264.7 细胞系作为破骨细胞的前体,这些细胞接受了 808 纳米 LLLT 的照射。通过耐酒石酸磷酸酶(TRAP)染色评估破骨细胞的分化情况。功能基因表达水平通过实时定量聚合酶链反应(RT-qPCR)进行评估,信号分子则通过 Western 印迹分析进行检测。在临床研究中,共有 12 名参与者参加。他们使用 TRIOS 台式扫描仪监测牙齿移动情况。骨密度测量使用 Mimics 软件进行,该软件可处理以数字成像和医学通信 (DICOM) 格式导出的锥束计算机断层扫描 (CBCT) 图像。结果我们发现,LLLT能有效促进RAW264.7细胞中核因子κB配体受体激活剂(RANKL)依赖性破骨细胞的分化和破骨细胞功能基因的表达,包括基质金属肽酶9(MMP9)、活化T细胞胞浆核因子1(NFATc1)、抗酒石酸磷酸酶(TRAP)和凝血酶K(CTSK)。结论:我们的研究表明,LLLT 不仅能显著促进破骨细胞的分化,还是正畸治疗中的一种重要辅助手段。
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引用次数: 0
Marginal fit and retention force of zirconia resin-bonded fixed dental prostheses in the posterior region with different designs 不同设计的氧化锆树脂粘结固定义齿在后牙区的边缘密合度和固位力
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.12.010
Yue Yin , Kosuke Nozaki , Reina Nemoto , Omnia Saleh , Yayoi Oishi , Mayuko Matsumura , Wataru Komada , Hiroyuki Miura , Kenji Fueki

Background/purpose

Retainer debonding of resin-bonded fixed dental prostheses (RBFDPs) is one of the major reasons for their lower survival rates than fixed dental prostheses (FDPs) with full-coverage crowns. Recent advances in milling technology have enabled the fabrication of RBFDPs with complex retainers (D-shaped designs). This study aimed to assess the marginal fit and retention force of zirconia RBFDPs with inlay-, L-, and D-shaped designs to clarify their clinical applications.

Materials and methods

Three abutment teeth models without maxillary second premolars were created using inlay-, L-, and D-shaped retainer designs. The zirconia RBFDPs were designed and fabricated according to the manufacturer’s instructions (n = 10). The marginal gap was measured using the silicone replica technique. Zirconia frameworks were bonded to the abutment teeth using resin cement. Tensile test was conducted after thermal cycling and dynamic loading tests. The loads during debonding or fracture were recorded. The failure pattern was analyzed by observing the fracture surface using a scanning electron microscope.

Results

D-shaped RBFDPs showed a significantly larger marginal gap than inlay- and L-shaped RBFDPs (P < 0.05). However, the mean marginal values were clinically acceptable (<120 μm). The D-shaped model exhibited the highest tensile strength in the tensile tests. The inlay-shaped and most of the D-shaped RBFDPs experienced debonding with cohesive failure, whereas the L-shaped RBFDPs showed fractures near the connector.

Conclusion

The D-shaped retainer design was superior to the inlay- and L-shaped designs with respect to the inhibition of retainer debonding. However, the marginal fitness needs to be improved.

背景/目的树脂粘结固定义齿(RBFDP)的固位体脱粘是其存活率低于全覆盖冠固定义齿(FDP)的主要原因之一。最近铣削技术的进步使得制作带有复杂固位体(D 型设计)的 RBFDP 成为可能。本研究旨在评估镶嵌式、L型和D型设计的氧化锆RBFDP的边缘密合度和固位力,以明确其临床应用。材料和方法使用镶嵌式、L型和D型固位体设计制作了三个无上颌第二前磨牙的基牙模型。氧化锆 RBFDP 按照制造商的说明进行设计和制作(n = 10)。使用硅胶复制技术测量边缘间隙。使用树脂粘结剂将氧化锆框架粘结到基牙上。热循环和动态加载测试后进行拉伸测试。记录脱粘或断裂时的载荷。结果D型RBFDP的边缘间隙明显大于嵌体型和L型RBFDP(P< 0.05)。不过,平均边缘值在临床上是可以接受的(<120 μm)。在拉伸试验中,D 型模型的拉伸强度最高。结论在抑制固位体脱落方面,D 型固位体设计优于嵌体和 L 型设计。但是,边缘适配性还需要改进。
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引用次数: 0
The potential positive effect of periodontal treatment on brain function activity using functional magnetic resonance imaging analysis 利用功能磁共振成像分析牙周治疗对大脑功能活动的潜在积极影响
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.09.024
Kosuke Muraoka , Masafumi Oda , Kenichi Yoshino , Tatsurou Tanaka , Masaki Morishita , Taiji Nakamura , Ryota Kibune , Kazuo Sonoki , Yasuhiro Morimoto , Keisuke Nakashima , Shuji Awano

Background/purpose

There are reports on the relationship between periodontal treatment and the whole body. The purpose of the present study was to investigate the effect of periodontal initial treatment on brain function activity by improving periodontal tissue and the occlusal status of subjects with periodontitis.

Materials and methods

The subjects were 13 patients with periodontitis. Following the patient's informed written consent, the periodontal initial treatment provided to the patient included tooth brushing instruction, scaling and root planning, however, occlusal adjustment was not performed at this stage. Periodontal examination, occlusal force examination and fMRI results were also evaluated at the initial and the reevaluation examinations.

Results

After the periodontal initial treatment had been performed, periodontal tissue had significantly improved. In addition, cerebral blood flow in the insula and primary motor cortex was also improved, as confirmed by fMRI.

Conclusion

This result suggests that the periodontal ligament has recovered and the periodontal ligament neuron have been further subjected to clenching in the insula.

背景/目的有报道称牙周治疗与全身的关系密切。本研究的目的是通过改善牙周组织和牙周炎患者的咬合状况,研究牙周初始治疗对大脑功能活动的影响。在获得患者知情同意书后,为患者提供的牙周初始治疗包括刷牙指导、洗牙和根面规划,但在此阶段不进行咬合调整。在初次检查和复查时,还对牙周检查、咬合力检查和 fMRI 结果进行了评估。结论这一结果表明,牙周韧带已经恢复,牙周韧带神经元进一步受到了岛叶挛缩的影响。
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引用次数: 0
A bibliometric analysis of teledentistry published in the category of dentistry, oral surgery and medicine 对牙科、口腔外科和医学类别中发表的远程齿科文献进行文献计量分析
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.016
Ting-Chia Liu , Yu-Chao Chang

Background/purpose

Telemedicine has gained the popularity during COVID-19 pandemic. “Teledentistry” as the dental application of telemedicine was also with increased attention. The bibliometric analysis was employed to examine the global research trends and the current implementation status of teledentistry.

Materials and methods

Titles and subjects were searched in the Web of Science database by using the keywords “teledentistry OR oral telemedicine OR dental telemedicine OR telemedicine in dentistry” in the category of Dentistry, Oral Surgery & Medicine. Documents were collected from the establishment date up to December 31, 2023. Microsoft Excel was used for the descriptive and statistical analyses. The data were exhibited with visualization by VOSviewer.

Results

A total of 146 articles were identified for bibliometric analysis. An upward trend in the number of publications was evident. The statistical analysis indicated a notable increased teledentistry publications affected by COVID-19 pandemic (P < 0.01). The applications of teledentistry were mainly related to clinical practice (89.73%), such as oral and maxillofacial surgery, orthodontics, dental caries, oral mucosal lesions, and dental emergencies. For geographic distributions, the United States lead with 46 publications (34%) by corresponding authors listed in the article. The keyword network analysis highlighted the prominent research areas and the changes influenced by the pandemic and technological development, respectively.

Conclusion

This bibliometric study provided an overview of the progress, trends and current directions for teledentistry in the fields of dentistry, oral surgery and medicine.

背景/目的在 COVID-19 大流行期间,远程医疗得到了普及。"远程牙科 "作为远程医疗的牙科应用也受到越来越多的关注。材料和方法在 Web of Science 数据库中,以 "teledentistry OR oral telemedicine OR dental telemedicine OR telemedicine in dentistry "为关键词,在牙科、口腔外科及医学类别中检索标题和主题。收集的文件从建立之日起至 2023 年 12 月 31 日。使用 Microsoft Excel 进行描述性分析和统计分析。通过 VOSviewer 对数据进行了可视化展示。论文数量呈明显上升趋势。统计分析表明,受 COVID-19 大流行的影响,远程电学出版物明显增加(P < 0.01)。远程口腔医学的应用主要与临床实践有关(89.73%),如口腔颌面外科、正畸、龋齿、口腔粘膜病变和牙科急诊。在地域分布方面,美国居首位,发表了 46 篇文章(占 34%),作者均为文章中列出的通讯作者。关键词网络分析分别突出了受流行病和技术发展影响的突出研究领域和变化。 结论这项文献计量学研究概述了牙科、口腔外科和医学领域远程医疗的进展、趋势和当前方向。
{"title":"A bibliometric analysis of teledentistry published in the category of dentistry, oral surgery and medicine","authors":"Ting-Chia Liu ,&nbsp;Yu-Chao Chang","doi":"10.1016/j.jds.2024.05.016","DOIUrl":"10.1016/j.jds.2024.05.016","url":null,"abstract":"<div><h3>Background/purpose</h3><p>Telemedicine has gained the popularity during COVID-19 pandemic. “Teledentistry” as the dental application of telemedicine was also with increased attention. The bibliometric analysis was employed to examine the global research trends and the current implementation status of teledentistry.</p></div><div><h3>Materials and methods</h3><p>Titles and subjects were searched in the Web of Science database by using the keywords “teledentistry OR oral telemedicine OR dental telemedicine OR telemedicine in dentistry” in the category of Dentistry, Oral Surgery &amp; Medicine. Documents were collected from the establishment date up to December 31, 2023. Microsoft Excel was used for the descriptive and statistical analyses. The data were exhibited with visualization by VOSviewer.</p></div><div><h3>Results</h3><p>A total of 146 articles were identified for bibliometric analysis. An upward trend in the number of publications was evident. The statistical analysis indicated a notable increased teledentistry publications affected by COVID-19 pandemic (<em>P</em> &lt; 0.01). The applications of teledentistry were mainly related to clinical practice (89.73%), such as oral and maxillofacial surgery, orthodontics, dental caries, oral mucosal lesions, and dental emergencies. For geographic distributions, the United States lead with 46 publications (34%) by corresponding authors listed in the article. The keyword network analysis highlighted the prominent research areas and the changes influenced by the pandemic and technological development, respectively.</p></div><div><h3>Conclusion</h3><p>This bibliometric study provided an overview of the progress, trends and current directions for teledentistry in the fields of dentistry, oral surgery and medicine.</p></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"19 3","pages":"Pages 1827-1833"},"PeriodicalIF":3.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224001624/pdfft?md5=ca28ce389b87c755bbe191febef5996f&pid=1-s2.0-S1991790224001624-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141140423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial efficacy and osteogenic potential of mineral trioxide aggregate-based retrograde filling material incorporated with silver nanoparticle and calcium fluoride 加入纳米银粒子和氟化钙的三氧化二矿骨料逆行填充材料的抗菌功效和成骨潜力
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.10.001
Min-Yong Lee , Hi-Won Yoon , Kwang-Mahn Kim , Jae-Sung Kwon

Background/purpose

The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF2) in retrograde filling material of MTA.

Materials and methods

MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF2, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF2. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against Enterococcus faecalis (E. faecalis) and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.

Results

When comparing the levels of antibacterial efficacy, the order of effectiveness was G4>G2>G3>G1 (P < 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (P < 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (P < 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (P < 0.05).

Conclusion

The addition of AgNPs and CaF2 to MTA could be a promising option for use as a new retrograde filling material.

背景/目的根尖切除术中使用的逆行充填材料,尤其是三氧化二铝矿物骨料(MTA),应具有较高的抗菌功效和成骨潜力。我们评估了在 MTA 逆行充填材料中添加纳米银(AgNPs)和氟化钙(CaF2)后的抗菌效果、生物相容性和成骨潜力。第一组(G1):蒸馏水;第二组(G2):50 ppm AgNPs;第三组(G3):50 ppm AgNPs:50 ppm AgNPs;第 3 组(G3):1 wt% CaF2;第 4 组(G3):1 wt% AgNPs:1 wt% CaF2,以及第 4 组(G4):50 ppm AgNPs 和 1 wt% CaF2。监测了各组的 pH 值变化,同时测量了表面粗糙度。使用比色法评估了各组对粪肠球菌(E. faecalis)的抗菌效果和小鼠前成骨细胞(MC3T3)的活力。各组 MC3T3 细胞成骨潜能标志物(OCN、ALPL 和 RUNX2)的基因表达水平采用实时 qPCR 法进行量化。结果比较各组的抗菌效果,效果依次为 G4>G2>G3>G1 (P < 0.05)。在细胞存活率测试中,由于 MTA 稀释生长培养基对 MC3T3 细胞增殖有积极影响,因此与对照组相比,G1-4 的细胞存活率在统计学上有所提高(P <0.05)。然而,与 G1 相比,G2-4 在统计学上没有显著差异(P < 0.05)。结论在 MTA 中添加 AgNPs 和 CaF2 可作为一种新型逆行充填材料,是一种很有前景的选择。
{"title":"Antibacterial efficacy and osteogenic potential of mineral trioxide aggregate-based retrograde filling material incorporated with silver nanoparticle and calcium fluoride","authors":"Min-Yong Lee ,&nbsp;Hi-Won Yoon ,&nbsp;Kwang-Mahn Kim ,&nbsp;Jae-Sung Kwon","doi":"10.1016/j.jds.2023.10.001","DOIUrl":"10.1016/j.jds.2023.10.001","url":null,"abstract":"<div><h3>Background/purpose</h3><p>The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF<sub>2</sub>) in retrograde filling material of MTA.</p></div><div><h3>Materials and methods</h3><p>MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF<sub>2</sub>, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF<sub>2</sub>. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against <em>Enterococcus faecalis (E. faecalis)</em> and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.</p></div><div><h3>Results</h3><p>When comparing the levels of antibacterial efficacy, the order of effectiveness was G4&gt;G2&gt;G3&gt;G1 (<em>P</em> &lt; 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (<em>P</em> &lt; 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (<em>P</em> &lt; 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>The addition of AgNPs and CaF<sub>2</sub> to MTA could be a promising option for use as a new retrograde filling material.</p></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"19 3","pages":"Pages 1783-1791"},"PeriodicalIF":3.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790223003367/pdfft?md5=ff4fa48d97abd875da151f98409b0f6a&pid=1-s2.0-S1991790223003367-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135656858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predentin's influence on clastic cell behavior in human external cervical resorption: Evidence from a case study Predentin 对人类宫颈外口吸收过程中碎屑细胞行为的影响:病例研究的证据
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.04.023
Toshihiro Noma , Shohei Yoshimoto , Yasuhiko Kamura , Masaki Arioka

External cervical resorption (ECR) is an aggressive disease characterized by resorption of the tooth root structure. While the pericanalar resorption-resistant sheet (PRRS) impedes ECR progression towards the pulp, the underlying mechanisms of its protective role in human teeth remain unclear. This study aimed to elucidate the pathology of ECR in a 31-year-old female patient by employing radiographic, histological, and immunohistochemical analyses of an extracted tooth. Histological examination revealed that the PRRS comprised dentin, predentin, and reparative bone-like tissue. Notably, clastic cells were observed on the surfaces of all three tissues within the same specimens. Immunohistochemical staining for cathepsin K demonstrated diminished resorptive activity of clastic cells on predentin compared to dentin and bone-like tissue. These findings suggest a potential role for predentin in attenuating clastic cell activity, potentially serving as the final barrier safeguarding the pulp tissue.

外牙颈部吸收(ECR)是一种以牙根结构吸收为特征的侵袭性疾病。虽然牙周抗吸收片(PRRS)能阻止 ECR 向牙髓发展,但其在人类牙齿中发挥保护作用的基本机制仍不清楚。本研究旨在通过对一颗拔出的牙齿进行放射学、组织学和免疫组化分析,阐明一位 31 岁女性患者的 ECR 病理学。组织学检查显示,PRRS 由牙本质、前牙本质和修复骨样组织组成。值得注意的是,在同一标本的所有三种组织表面都观察到了碎屑细胞。cathepsin K免疫组化染色显示,与牙本质和类骨组织相比,前牙本质上的碎屑细胞的吸收活性减弱。这些研究结果表明,牙本质在减弱碎屑细胞活性方面具有潜在的作用,有可能成为保护牙髓组织的最后一道屏障。
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引用次数: 0
Partial regression of a healed periapical lesion in an endodontically treated premolar during orthodontic extrusion: A case report 一颗经牙髓治疗的前磨牙在正畸挤压过程中出现部分愈合的根尖周病变:病例报告
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.005
He Liu, Jing Hao, Ya Shen
{"title":"Partial regression of a healed periapical lesion in an endodontically treated premolar during orthodontic extrusion: A case report","authors":"He Liu,&nbsp;Jing Hao,&nbsp;Ya Shen","doi":"10.1016/j.jds.2024.05.005","DOIUrl":"10.1016/j.jds.2024.05.005","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"19 3","pages":"Pages 1894-1896"},"PeriodicalIF":3.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S199179022400151X/pdfft?md5=80a7cb16347fff593a4a9bfa7b0bf02a&pid=1-s2.0-S199179022400151X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141033442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intraoral myoepithelial carcinoma in a young man with intellectual disability: A highly rare and challenging case 一名智障青年的口腔内肌上皮癌:一个非常罕见且具有挑战性的病例
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.015
Ke-Hsuan Liu, Kung-Yu Shao
{"title":"Intraoral myoepithelial carcinoma in a young man with intellectual disability: A highly rare and challenging case","authors":"Ke-Hsuan Liu,&nbsp;Kung-Yu Shao","doi":"10.1016/j.jds.2024.05.015","DOIUrl":"10.1016/j.jds.2024.05.015","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"19 3","pages":"Pages 1888-1889"},"PeriodicalIF":3.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224001612/pdfft?md5=b13b212ac37220bd51ad50accda990ff&pid=1-s2.0-S1991790224001612-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141135330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Involvement of miR-199a-5p-loaded mesoporous silica nanoparticle-polyethyleneimine-KALA in osteogenic differentiation miR-199a-5p负载介孔二氧化硅纳米粒子-聚乙烯亚胺-KALA参与成骨分化过程
IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.01.007
Tianyue Wang , Hidemi Nakata , Bing Shen , Ziying Jiao , Kaori Yokota , Shinji Kuroda , Shohei Kasugai , Eriko Marukawa

Background/purpose

While there are numerous reports on surgical techniques and materials for bone grafting, limited methods are available to enhance the body's inherent capacity to heal bones. Here we investigated microRNA-199a (miR-199a), a molecular that promotes osteoblast differentiation and bone healing.

Materials and methods

To construct a miR-199a delivery complex, miR-199a-5p mimics were coated with mesoporous silica nanoparticles (MSNs) following modified with polyethyleneimine (PEI) and peptide WEAKLAKALAKALAKHLAKALAKALKACEA (KALA) to obtain 199a-5p-loaded MSN-PEI-KALA. Nanoparticle complexes are assessed for particle size and zeta potential using transmission electron microscopy and dynamic light scattering. Then MC3T3-E1 cells are exposed to MSN_miR-199a-5p @PEI-KALA. The impact of MSN_miR-199a-5p@PEI-KALA at varying concentrations on cell viability is assessed using Cell Counting Kit-8. Cell uptake and distribution were analyzed by double fluorescent staining with fluorescein amidite-labeled MSN_miR-199a@PEI-KALA and lysosome labeling. On day 7 after osteogenic induction, alkaline phosphatase (ALP) staining was conducted.

Results

The findings indicated that the nanoparticle complexes encapsulating PEI and peptide exhibited an augmentation in both particle size and zeta potential. At a dosage of 10 μg/mL, MSN_miR-199a@PEI-KALA displayed the lowest cytotoxicity compared to the control group. MC3T3-E1 cells treated with MSN_miR-199a-5p@PEI-KALA exhibited intensified ALP staining and elevated mRNA expression levels of ALP, runt-related transcription factor 2, and osteopontin, suggesting the involvement of miR-199a-5p-loaded MSN-PEI-KALA in osteogenic differentiation.

Conclusion

The successful construction of the delivering complex MSN_miR-199a@PEI-KALA in present research highlights the promise of this biomaterial carrier for the application of miRNAs in treating bone defects.

背景/目的尽管有关骨移植手术技术和材料的报道不胜枚举,但用于增强人体固有的骨骼愈合能力的方法却十分有限。材料与方法为了构建 miR-199a 递送复合物,miR-199a-5p 模拟物被包覆在介孔二氧化硅纳米颗粒(MSNs)上,然后用聚乙烯亚胺(PEI)和肽 WEAKLAKALAKALAKHLAKALAKALKACEA(KALA)修饰,得到 199a-5p 负载的 MSN-PEI-KALA。利用透射电子显微镜和动态光散射评估纳米颗粒复合物的粒度和 ZETA 电位。然后将 MC3T3-E1 细胞暴露于 MSN_miR-199a-5p @PEI-KALA 中。使用细胞计数试剂盒-8 评估不同浓度的 MSN_miR-199a-5p@PEI-KALA 对细胞活力的影响。通过亚氨酸荧光素标记的MSN_miR-199a@PEI-KALA和溶酶体标记的双重荧光染色分析细胞摄取和分布情况。结果研究结果表明,包裹 PEI 和多肽的纳米颗粒复合物的粒径和 ZETA 电位都有所增大。与对照组相比,当剂量为 10 μg/mL 时,MSN_miR-199a@PEI-KALA 的细胞毒性最低。用MSN_miR-199a-5p@PEI-KALA处理的MC3T3-E1细胞的ALP染色增强,ALP、runt相关转录因子2和骨素的mRNA表达水平升高,表明miR-199a-5p负载的MSN-PEI-KALA参与了成骨分化。结论本研究成功构建了MSN_miR-199a@PEI-KALA递送复合物,凸显了这种生物材料载体在应用miRNA治疗骨缺损方面的前景。
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引用次数: 0
期刊
Journal of Dental Sciences
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