Journal of Dental Sciences最新文献_第9页

Profiles of peripheral B cell subsets in a cohort of primary Sjögren's syndrome patients and their potential clinical significance 一组原发性斯约格伦综合征患者的外周 B 细胞亚群特征及其潜在临床意义

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.12.024

Yixiao Xing , Boya Li , Pan Wei , Hong Hua

Background/purpose

Primary Sjögren's syndrome is a prototypical autoimmune disease, with B cell dysfunction as a dominant feature. Further insights into distribution of B cell subsets in primary Sjögren's syndrome are urgently required to identify the most appropriate target subpopulation. We aimed to evaluate the profiles of B lymphocyte subpopulations in primary Sjögren's syndrome patients and to investigate their clinical significance.

Materials and methods

Thirty primary Sjögren's syndrome patients and 15 age-and sex-matched healthy controls were recruited. Peripheral B cell subsets were analyzed by flow cytometry.

Results

Compared to healthy controls, circulating CD19⁺ B cells, CD19⁺CD20⁻ B cells, CD19⁺CD27⁻IgD⁺ naïve B cells, CD19⁺IgD⁺CD38^high plasmablasts, CD19⁺CD24^highCD38^high transitional B cells and CD19⁺CD20⁻CD27⁺CD38⁺ plasma cells were elevated in patients with primary Sjögren's syndrome, whereas CD19⁺CD27⁺ memory B cells, CD19⁺CD27⁻IgD^- double negative B cells and CD19⁺CD24^hiCD27⁺ Bregs were decreased. Furthermore, the percentage of circulating CD19⁺CD20⁻CD27⁺CD38⁺ plasma cells was positively correlated with serum IgG levels and the proportional area of lymphocytic infiltration of labial gland.

Conclusion

We identified a comprehensive B lymphocyte subset distribution profile in primary Sjögren's syndrome. Moreover, we detected a clinical significance of CD19⁺CD20⁻CD27⁺CD38⁺ plasma cells, suggesting that these cells might play a key role in disease pathology and represent potential therapeutic targets.

背景/目的原发性斯约格伦综合征是一种典型的自身免疫性疾病，以 B 细胞功能障碍为主要特征。我们迫切需要进一步了解原发性斯约格伦综合征中 B 细胞亚群的分布情况，以确定最合适的目标亚群。我们的目的是评估原发性斯约格伦综合征患者 B 淋巴细胞亚群的分布情况，并研究其临床意义。流式细胞术分析了外周 B 细胞亚群。而 CD19+CD27+ 记忆 B 细胞、CD19+CD27-IgD- 双阴性 B 细胞和 CD19+CD24hiCD27+ Bregs 则减少。此外，循环中 CD19+CD20-CD27+CD38+ 浆细胞的百分比与血清 IgG 水平和唇腺淋巴细胞浸润的比例面积呈正相关。此外，我们还发现了 CD19+CD20-CD27+CD38+ 浆细胞的临床意义，这表明这些细胞可能在疾病病理过程中起着关键作用，是潜在的治疗靶点。

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引用次数: 0

Novel NRAS G12D mutation in extraosseous/peripheral ameloblastoma: Case report and review of mitogen-activated protein kinase pathway mutations in extraosseous/peripheral and intraosseous ameloblastomas 骨外/外周骨髓母细胞瘤中的新型NRAS G12D突变：病例报告及骨外/外周和骨内骨髓母细胞瘤中丝裂原活化蛋白激酶通路突变的综述

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.010

Kyu-Young Oh, Seong-Doo Hong

引用次数: 0

Gastric adenocarcinoma predictive long intergenic noncoding RNA (GAPLINC) promotes oral cancer stemness by acting as a molecular sponge of miR331-3p 胃腺癌预测性长基因间非编码 RNA (GAPLINC) 通过充当 miR331-3p 的分子海绵促进口腔癌干细胞的形成

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.04.031

Kuang-Yuan Liang , Ni-Yu Su , Hsiu-Pin Yang , Pei-Ling Hsieh , Chih-Yuan Fang , Lo-Lin Tsai , Yi-Wen Liao , Chia-Ming Liu , Cheng-Chia Yu

Background/purpose

Accumulating evidence has suggested that treatment failure of cancer therapy can be attributed to cancer stem cells (CSCs). Among numerous regulators of cancer stemness, non-coding RNAs (ncRNAs) have gained significant attention recently. In this study, we examined the role of gastric adenocarcinoma predictive long intergenic noncoding RNA (GAPLINC) in oral CSCs (OCSCs).

Materials and methods

RNA Sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were used to determine the expression of GAPLINC. Flow cytometry and sphere-forming assay were exploited to isolate OCSCs. Measurement of aldehyde dehydrogenase 1 (ALDH1) activity, CD44 expressing cells, and various phenotypic assays, such as self-renewal, migration, invasion, and colony-forming abilities, were conducted in CSCs of two types of oral cancer cells (SAS and GNM) following the knockdown of GAPLINC. A luciferase reporter was also carried out to validate the direct interaction between GAPLINC and microRNA (miR)-331-3p.

Results

Our results showed that GAPLINC was overexpressed in OCSCs from patient-derived and oral cancer cell lines. We demonstrated that silencing of GAPLINC in OCSCs downregulated various CSC hallmarks, such as ALDH1 activity, percentage of CD44-expressing cells, self-renewal capacity, and colony-forming ability. Moreover, our results revealed that the effect of GAPLINC on cancer stemness was mediated by direct repression of miR-331-3p.

Conclusion

These data have potential clinical implications in that we unraveled the aberrant upregulation of GAPLINC and demonstrated that suppression of GAPLINC may reduce cancer stemness via sequestering miR-331-3p.

背景/目的越来越多的证据表明，癌症治疗失败可归因于癌症干细胞（CSCs）。在癌症干细胞的众多调控因子中，非编码 RNA（ncRNA）近来受到了广泛关注。在这项研究中，我们研究了胃腺癌预测性长基因间非编码RNA（GAPLINC）在口腔CSCs（OCSCs）中的作用。材料与方法RNA测序和定量实时聚合酶链反应（qRT-PCR）用于确定GAPLINC的表达。流式细胞术和球形成试验用于分离口腔癌细胞。在敲除 GAPLINC 后，对两种口腔癌细胞（SAS 和 GNM）的 CSCs 进行了醛脱氢酶 1 (ALDH1) 活性、CD44 表达细胞和各种表型检测，如自我更新、迁移、侵袭和集落形成能力。我们的研究结果表明，GAPLINC 在患者来源的口腔癌细胞系和口腔癌细胞系的 OCSCs 中过表达。我们的研究结果表明，GAPLINC 在患者来源的口腔癌细胞系和口腔癌细胞系的 OCSCs 中过表达，而在 OCSCs 中沉默 GAPLINC 会降低各种 CSC 标志，如 ALDH1 活性、CD44 表达细胞百分比、自我更新能力和集落形成能力。结论这些数据具有潜在的临床意义，因为我们揭示了GAPLINC的异常上调，并证明抑制GAPLINC可通过封闭miR-331-3p降低癌症干性。

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引用次数: 0

Soft coral Lobophytum crassum extract inhibits migration and induces apoptosis capabilities in human oral squamous cell carcinoma cells 软珊瑚 Lobophytum crassum 提取物抑制人口腔鳞状细胞癌细胞迁移并诱导其凋亡的能力

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.06.016

Shih-Min Wang, Mei-Chin Lu, Yu-Ming Hsu, Chun-Chung Huang, Chih-Yuan Fang, Chuan-Hang Yu, Chueh Pin Ju, Chengen Yu, Shiao-Pieng Lee

引用次数: 0

The potential positive effect of periodontal treatment on brain function activity using functional magnetic resonance imaging analysis 利用功能磁共振成像分析牙周治疗对大脑功能活动的潜在积极影响

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.09.024

Kosuke Muraoka , Masafumi Oda , Kenichi Yoshino , Tatsurou Tanaka , Masaki Morishita , Taiji Nakamura , Ryota Kibune , Kazuo Sonoki , Yasuhiro Morimoto , Keisuke Nakashima , Shuji Awano

Background/purpose

There are reports on the relationship between periodontal treatment and the whole body. The purpose of the present study was to investigate the effect of periodontal initial treatment on brain function activity by improving periodontal tissue and the occlusal status of subjects with periodontitis.

Materials and methods

The subjects were 13 patients with periodontitis. Following the patient's informed written consent, the periodontal initial treatment provided to the patient included tooth brushing instruction, scaling and root planning, however, occlusal adjustment was not performed at this stage. Periodontal examination, occlusal force examination and fMRI results were also evaluated at the initial and the reevaluation examinations.

Results

After the periodontal initial treatment had been performed, periodontal tissue had significantly improved. In addition, cerebral blood flow in the insula and primary motor cortex was also improved, as confirmed by fMRI.

Conclusion

This result suggests that the periodontal ligament has recovered and the periodontal ligament neuron have been further subjected to clenching in the insula.

背景/目的有报道称牙周治疗与全身的关系密切。本研究的目的是通过改善牙周组织和牙周炎患者的咬合状况，研究牙周初始治疗对大脑功能活动的影响。在获得患者知情同意书后，为患者提供的牙周初始治疗包括刷牙指导、洗牙和根面规划，但在此阶段不进行咬合调整。在初次检查和复查时，还对牙周检查、咬合力检查和 fMRI 结果进行了评估。结论这一结果表明，牙周韧带已经恢复，牙周韧带神经元进一步受到了岛叶挛缩的影响。

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引用次数: 0

Antibacterial efficacy and osteogenic potential of mineral trioxide aggregate-based retrograde filling material incorporated with silver nanoparticle and calcium fluoride 加入纳米银粒子和氟化钙的三氧化二矿骨料逆行填充材料的抗菌功效和成骨潜力

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.10.001

Min-Yong Lee , Hi-Won Yoon , Kwang-Mahn Kim , Jae-Sung Kwon

Background/purpose

The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF₂) in retrograde filling material of MTA.

Materials and methods

MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF₂, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF₂. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against Enterococcus faecalis (E. faecalis) and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.

Results

When comparing the levels of antibacterial efficacy, the order of effectiveness was G4>G2>G3>G1 (P < 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (P < 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (P < 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (P < 0.05).

Conclusion

The addition of AgNPs and CaF₂ to MTA could be a promising option for use as a new retrograde filling material.

背景/目的根尖切除术中使用的逆行充填材料，尤其是三氧化二铝矿物骨料（MTA），应具有较高的抗菌功效和成骨潜力。我们评估了在 MTA 逆行充填材料中添加纳米银（AgNPs）和氟化钙（CaF2）后的抗菌效果、生物相容性和成骨潜力。第一组（G1）：蒸馏水；第二组（G2）：50 ppm AgNPs；第三组（G3）：50 ppm AgNPs：50 ppm AgNPs；第 3 组（G3）：1 wt% CaF2；第 4 组（G3）：1 wt% AgNPs：1 wt% CaF2，以及第 4 组（G4）：50 ppm AgNPs 和 1 wt% CaF2。监测了各组的 pH 值变化，同时测量了表面粗糙度。使用比色法评估了各组对粪肠球菌（E. faecalis）的抗菌效果和小鼠前成骨细胞（MC3T3）的活力。各组 MC3T3 细胞成骨潜能标志物（OCN、ALPL 和 RUNX2）的基因表达水平采用实时 qPCR 法进行量化。结果比较各组的抗菌效果，效果依次为 G4>G2>G3>G1 (P < 0.05)。在细胞存活率测试中，由于 MTA 稀释生长培养基对 MC3T3 细胞增殖有积极影响，因此与对照组相比，G1-4 的细胞存活率在统计学上有所提高（P <0.05）。然而，与 G1 相比，G2-4 在统计学上没有显著差异（P < 0.05）。结论在 MTA 中添加 AgNPs 和 CaF2 可作为一种新型逆行充填材料，是一种很有前景的选择。

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引用次数: 0

Marginal fit and retention force of zirconia resin-bonded fixed dental prostheses in the posterior region with different designs 不同设计的氧化锆树脂粘结固定义齿在后牙区的边缘密合度和固位力

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.12.010

Yue Yin , Kosuke Nozaki , Reina Nemoto , Omnia Saleh , Yayoi Oishi , Mayuko Matsumura , Wataru Komada , Hiroyuki Miura , Kenji Fueki

Background/purpose

Retainer debonding of resin-bonded fixed dental prostheses (RBFDPs) is one of the major reasons for their lower survival rates than fixed dental prostheses (FDPs) with full-coverage crowns. Recent advances in milling technology have enabled the fabrication of RBFDPs with complex retainers (D-shaped designs). This study aimed to assess the marginal fit and retention force of zirconia RBFDPs with inlay-, L-, and D-shaped designs to clarify their clinical applications.

Materials and methods

Three abutment teeth models without maxillary second premolars were created using inlay-, L-, and D-shaped retainer designs. The zirconia RBFDPs were designed and fabricated according to the manufacturer’s instructions (n = 10). The marginal gap was measured using the silicone replica technique. Zirconia frameworks were bonded to the abutment teeth using resin cement. Tensile test was conducted after thermal cycling and dynamic loading tests. The loads during debonding or fracture were recorded. The failure pattern was analyzed by observing the fracture surface using a scanning electron microscope.

Results

D-shaped RBFDPs showed a significantly larger marginal gap than inlay- and L-shaped RBFDPs (P < 0.05). However, the mean marginal values were clinically acceptable (<120 μm). The D-shaped model exhibited the highest tensile strength in the tensile tests. The inlay-shaped and most of the D-shaped RBFDPs experienced debonding with cohesive failure, whereas the L-shaped RBFDPs showed fractures near the connector.

Conclusion

The D-shaped retainer design was superior to the inlay- and L-shaped designs with respect to the inhibition of retainer debonding. However, the marginal fitness needs to be improved.

背景/目的树脂粘结固定义齿（RBFDP）的固位体脱粘是其存活率低于全覆盖冠固定义齿（FDP）的主要原因之一。最近铣削技术的进步使得制作带有复杂固位体（D 型设计）的 RBFDP 成为可能。本研究旨在评估镶嵌式、L型和D型设计的氧化锆RBFDP的边缘密合度和固位力，以明确其临床应用。材料和方法使用镶嵌式、L型和D型固位体设计制作了三个无上颌第二前磨牙的基牙模型。氧化锆 RBFDP 按照制造商的说明进行设计和制作（n = 10）。使用硅胶复制技术测量边缘间隙。使用树脂粘结剂将氧化锆框架粘结到基牙上。热循环和动态加载测试后进行拉伸测试。记录脱粘或断裂时的载荷。结果D型RBFDP的边缘间隙明显大于嵌体型和L型RBFDP（P< 0.05）。不过，平均边缘值在临床上是可以接受的（<120 μm）。在拉伸试验中，D 型模型的拉伸强度最高。结论在抑制固位体脱落方面，D 型固位体设计优于嵌体和 L 型设计。但是，边缘适配性还需要改进。

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引用次数: 0

Novel regimens of phytopolyphenols and celecoxib enhancing efficacy and selectivity of anticancer effects of chemotherapeutic agents on cultured cancer cells 植物多酚和塞来昔布的新方案可提高化疗药物对培养癌细胞的抗癌效果和选择性

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.02.013

Ming-Yung Chou , Shoei-Yn Lin-Shiau

Background/purpose

Explorations of novel regimens enhancing efficacy and selectivity of chemotherapeutic agents are urgent to solve the problems of cancer therapy. This study aimed to explore synergistic anticancer effects of novel regimens of phytopolyphenols [curcumin (C), tea polyphenols (G) or GC] with celecoxib (Cl) and ZnSO₄.

Materials and methods

Antiproliferative effects of drugs on cultured cancer cells and pathogenic biofilms were assayed by MTT and optical density (OD600) respectively; their inhibition on efflux pump (Na⁺-K⁺-ATPase) was measured by colorimetric methods. Synergistic (CI < 1) anticancer effects were evaluated by the equations of combination index (CI) and efficacy index (EI).

Results

Both Cl and methotrexate (MTX) alone exhibited inhibitory effects not only on proliferation and efflux pump of cultured cancer cells but also pathogenic biofilm formation. Phytopolyphenols (P) and MTX potentiated these inhibitory effects of Cl. In addition, novel regimens containing Cl, memantine (Mem) or thioridazine (TRZ) further enhanced not only efficacy and selectivity of anticancer effects but also inhibition on efflux pump and pathogenic biofilm formation of four chemotherapeutic agents (MTX, cisplatin, 5-fluorouracil and doxorubicin) respectively.

Conclusion

In this study, novel regimens of phytopolyphenols (P), targeting drugs (T; Cl, Mem or TRZ) and metal ions (M; ZnSO₄) so called PTM regimens exerted not only by themselves but also markedly potentiated efficacy and selectivity of anticancer effects of four chemotherapeutic agents. Because of their potent inhibitions on efflux pump and pathogenic biofilm formation, these combinatorial novel regimens were expected to be able to overcome the problems of multidrug resistant cancers and merit for further clinical studies.

探索提高化疗药物疗效和选择性的新型方案是解决癌症治疗问题的当务之急。本研究旨在探索植物多酚[姜黄素（C）、茶多酚（G）或 GC]与塞来昔布（Cl）和硫酸锌的协同抗癌效果。药物对培养的癌细胞和病原生物膜的抗增殖作用分别通过 MTT 和光密度（OD600）进行检测；对外流泵（Na-K-ATPase）的抑制作用则通过比色法进行检测。协同（CI < 1）抗癌效果通过组合指数（CI）和药效指数（EI）方程进行评估。Cl 和单独的甲氨蝶呤（MTX）不仅对培养癌细胞的增殖和外排泵有抑制作用，而且对致病生物膜的形成也有抑制作用。植物多酚（P）和 MTX 增强了 Cl 的这些抑制作用。此外，含有 Cl、美金刚（Mem）或硫利达嗪（TRZ）的新方案不仅进一步增强了抗癌效果和选择性，还分别增强了对四种化疗药物（MTX、顺铂、5-氟尿嘧啶和多柔比星）的外排泵和致病性生物膜形成的抑制作用。在这项研究中，由植物多酚（P）、靶向药物（T；Cl、Mem 或 TRZ）和金属离子（M；ZnSO）组成的新型方案（即 PTM 方案）不仅本身具有抑制作用，而且还显著增强了四种化疗药物的抗癌功效和选择性。由于这些组合新方案能有效抑制外排泵和病原体生物膜的形成，因此有望克服多药耐药性癌症的问题，值得进一步临床研究。

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引用次数: 0

Carotid artery calcification detected on the panoramic radiographs 全景照片上发现颈动脉钙化

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.04.005

Feng-Chou Cheng, Shu-Yu Liu, Bo-Lin Liu, Chun-Pin Chiang

引用次数: 0

The effect of low-level laser therapy on osteoclast differentiation: Clinical implications for tooth movement and bone density 低强度激光疗法对破骨细胞分化的影响：对牙齿移动和骨密度的临床影响

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.03.023

Chun-Yi Huang , Huynh Hoai Thuong Le , Hsiao-Chi Tsai , Chih-Hsin Tang , Jian-Hong Yu

Background/purpose

Osteoclast differentiation is crucial for orchestrating both tooth movement and the maintenance of bone density. Therefore, the current study sought to explore the impact of low-level laser therapy (LLLT) on osteoclast differentiation, functional gene expression, molecular signaling pathways, and orthodontic tooth movement in clinical settings.

Materials and methods

The RAW 264.7 cell line served as the precursor for osteoclasts, and these cells underwent irradiation using a 808-nm LLLT. Osteoclast differentiation was assessed through tartrate-resistant acid phosphatase (TRAP) staining. Functional gene expression levels were evaluated using real-time quantitative polymerase chain reaction (RT-qPCR) while signaling molecules were examined through Western blot analysis. In the clinical study, 12 participants were enrolled. Their tooth movement was monitored using a TRIOS desktop scanner. Bone density measurements were conducted using Mimics software, which processed cone-beam computed tomography (CBCT) images exported in Digital Imaging and Communications in Medicine (DICOM) format.

Results

We found that LLLT effectively promoted receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation and the expression of osteoclast functional genes, including matrix metallopeptidase 9 (MMP9), nuclear factor of activated T-cells cytoplasmic 1(NFATc1), tartrate-resistant acid phosphatase (TRAP) and cathepsin K (CTSK) in RAW264.7 cells. Clinically, the cumulative tooth movement over 90 days was significantly higher in the laser group than in the control group.

Conclusion

Our research demonstrates that LLLT not only significantly promotes osteoclast differentiation but is also a valuable adjunct in orthodontic therapy.

背景/目的破骨细胞的分化对于协调牙齿移动和维持骨密度至关重要。因此，本研究试图探讨低强度激光疗法（LLLT）对破骨细胞分化、功能基因表达、分子信号通路和临床正畸牙齿移动的影响。材料与方法 RAW 264.7 细胞系作为破骨细胞的前体，这些细胞接受了 808 纳米 LLLT 的照射。通过耐酒石酸磷酸酶（TRAP）染色评估破骨细胞的分化情况。功能基因表达水平通过实时定量聚合酶链反应（RT-qPCR）进行评估，信号分子则通过 Western 印迹分析进行检测。在临床研究中，共有 12 名参与者参加。他们使用 TRIOS 台式扫描仪监测牙齿移动情况。骨密度测量使用 Mimics 软件进行，该软件可处理以数字成像和医学通信 (DICOM) 格式导出的锥束计算机断层扫描 (CBCT) 图像。结果我们发现，LLLT能有效促进RAW264.7细胞中核因子κB配体受体激活剂（RANKL）依赖性破骨细胞的分化和破骨细胞功能基因的表达，包括基质金属肽酶9（MMP9）、活化T细胞胞浆核因子1（NFATc1）、抗酒石酸磷酸酶（TRAP）和凝血酶K（CTSK）。结论：我们的研究表明，LLLT 不仅能显著促进破骨细胞的分化，还是正畸治疗中的一种重要辅助手段。

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引用次数: 0