Adipose tissue is a key determinant of the sensory, nutritional, and functional qualities of meat, yet its faithful replication in vitro remains one of the central challenges for cultured meat production. This review first examines the roles of fat in conventional meat, encompassing its contributions to flavor, texture, and nutritional value through diverse lipid classes such as triacylglycerols, phospholipids, and cholesterol. The biological basis of adipogenesis is then outlined across the phases of proliferation, differentiation, and maturation, emphasizing transcriptional regulators, metabolic transitions, and lipid remodeling that collectively shape adipocyte development. Building on these foundations, insights from in vivo regulation—including species, sex, cuts, and dietary influences—are integrated with in vitro culture strategies. These integrations illustrate how physiological cues can inform the design of media formulations, signaling environments, and scaffolding platforms to enhance lipid composition and improve nutritional quality as well as co-culture strategies with myocytes to reproduce marbling-like structures. By bridging adipose biology with tissue engineering, this review establishes a framework for cultured fat design that meets the sensory and nutritional benchmarks of conventional adipose tissue while offering opportunities for functional tailoring. Finally, remaining challenges are briefly discussed as critical steps for translating laboratory progress into viable and socially accepted food applications.
{"title":"Integrative Adipogenic Engineering of Cultured Fat for Cell Based Meat","authors":"Young-Seung Lee, Hyun Jung Lee, Cheorun Jo","doi":"10.1111/1541-4337.70338","DOIUrl":"10.1111/1541-4337.70338","url":null,"abstract":"<p>Adipose tissue is a key determinant of the sensory, nutritional, and functional qualities of meat, yet its faithful replication in vitro remains one of the central challenges for cultured meat production. This review first examines the roles of fat in conventional meat, encompassing its contributions to flavor, texture, and nutritional value through diverse lipid classes such as triacylglycerols, phospholipids, and cholesterol. The biological basis of adipogenesis is then outlined across the phases of proliferation, differentiation, and maturation, emphasizing transcriptional regulators, metabolic transitions, and lipid remodeling that collectively shape adipocyte development. Building on these foundations, insights from in vivo regulation—including species, sex, cuts, and dietary influences—are integrated with in vitro culture strategies. These integrations illustrate how physiological cues can inform the design of media formulations, signaling environments, and scaffolding platforms to enhance lipid composition and improve nutritional quality as well as co-culture strategies with myocytes to reproduce marbling-like structures. By bridging adipose biology with tissue engineering, this review establishes a framework for cultured fat design that meets the sensory and nutritional benchmarks of conventional adipose tissue while offering opportunities for functional tailoring. Finally, remaining challenges are briefly discussed as critical steps for translating laboratory progress into viable and socially accepted food applications.</p>","PeriodicalId":155,"journal":{"name":"Comprehensive Reviews in Food Science and Food Safety","volume":"25 1","pages":""},"PeriodicalIF":14.1,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12673359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145660002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As food safety issues gain increasing attention, the need for rapid, accurate, and low-cost detection techniques becomes essential. Colorimetric sensors, known for their rapid response, high sensitivity, low cost, and readily observable results, are playing an increasingly important role in food quality inspection. This review article provides an in-depth discussion on the development and application of colorimetric sensing technology in the field of food detection and safety. The review outlines the fundamental principles of colorimetric sensors and provides a critical assessment of research on array dyes, substrates, and system optimization. In recent years, the optimization of functionalized precious metal nanoparticles and nanoenzymes has pushed detection limits down to the parts per billion level. Porous polymer substrates modified with synergistic flexible materials combine high adsorption, strong anti-interference, and excellent mechanical properties, providing support for integrated applications in wearable sensors and smart packaging. The integration of data acquisition and analysis techniques based on color changes is also discussed. Through an overview of the application of sensors in food safety testing, food quality control, and food production and processing monitoring, this review article highlights the significant potential of colorimetric sensor arrays (CSAs) in enhancing food safety and quality. The review also identifies current challenges, with major obstacles including environmental interference, matrix effects, and limited industrial validation. The review concludes that combining advanced sensor materials, portable and stable optical devices with online analytical platforms for real-time data extraction and simple analysis will enable CSAs to develop into a powerful on-site tool for ensuring food quality and safety.
{"title":"Integration of Colorimetric Sensing Dyes and Multi-Substrate Arrays for Food Quality and Safety Monitoring: Design, Optimization, and Analysis","authors":"Jingfei Shen, Qisheng Liu, Ruxin Zhang, Zhengzhu Zhang, Quansheng Chen, Jingming Ning, Luqing Li","doi":"10.1111/1541-4337.70344","DOIUrl":"10.1111/1541-4337.70344","url":null,"abstract":"<p>As food safety issues gain increasing attention, the need for rapid, accurate, and low-cost detection techniques becomes essential. Colorimetric sensors, known for their rapid response, high sensitivity, low cost, and readily observable results, are playing an increasingly important role in food quality inspection. This review article provides an in-depth discussion on the development and application of colorimetric sensing technology in the field of food detection and safety. The review outlines the fundamental principles of colorimetric sensors and provides a critical assessment of research on array dyes, substrates, and system optimization. In recent years, the optimization of functionalized precious metal nanoparticles and nanoenzymes has pushed detection limits down to the parts per billion level. Porous polymer substrates modified with synergistic flexible materials combine high adsorption, strong anti-interference, and excellent mechanical properties, providing support for integrated applications in wearable sensors and smart packaging. The integration of data acquisition and analysis techniques based on color changes is also discussed. Through an overview of the application of sensors in food safety testing, food quality control, and food production and processing monitoring, this review article highlights the significant potential of colorimetric sensor arrays (CSAs) in enhancing food safety and quality. The review also identifies current challenges, with major obstacles including environmental interference, matrix effects, and limited industrial validation. The review concludes that combining advanced sensor materials, portable and stable optical devices with online analytical platforms for real-time data extraction and simple analysis will enable CSAs to develop into a powerful on-site tool for ensuring food quality and safety.</p>","PeriodicalId":155,"journal":{"name":"Comprehensive Reviews in Food Science and Food Safety","volume":"25 1","pages":""},"PeriodicalIF":14.1,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145646984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Over the years, numerous D- and z-values have been published in the scientific literature. While these values initially appear to be valid criteria for assessing and comparing the heat resistance of different microorganisms under the same conditions or the same microorganism under different conditions, this is not always the case. This review presents a comprehensive overview of food safety in fruit processing, considering contamination routes and focusing on the selection of surrogates for key pathogens such as Salmonella spp. and Escherichia coli. Factors influencing heat resistance parameters, such as the experimental method, food matrix, mathematical model, and challenges of industrial upscaling, are explored. We critically assess the diversity and lack of methodological standardization in determining D- and z-values, showing the consequences for risk assessment and surrogate selection. Along with a review of methods used by scientists, the problem of different methods for calculating the decimal reduction time is highlighted, as well as the lack of specifications for the use of z-values. Additionally, this review proposes the use of the glass capillary method for testing bacterial thermal resistance in liquid foods, such as fruit juices and nectars, in order to standardize the methodology and facilitate comparisons across the literature.
{"title":"Facing the D-Ilemma of Heat Resistance Parameters: From Pathogen Risk Assessment to Surrogate Selection Challenges in the Fruit Juice and Nectar Production","authors":"Astrid Gędas, Agnes Weiss","doi":"10.1111/1541-4337.70346","DOIUrl":"10.1111/1541-4337.70346","url":null,"abstract":"<p>Over the years, numerous <i>D</i>- and <i>z</i>-values have been published in the scientific literature. While these values initially appear to be valid criteria for assessing and comparing the heat resistance of different microorganisms under the same conditions or the same microorganism under different conditions, this is not always the case. This review presents a comprehensive overview of food safety in fruit processing, considering contamination routes and focusing on the selection of surrogates for key pathogens such as <i>Salmonella</i> spp. and <i>Escherichia coli</i>. Factors influencing heat resistance parameters, such as the experimental method, food matrix, mathematical model, and challenges of industrial upscaling, are explored. We critically assess the diversity and lack of methodological standardization in determining <i>D</i>- and <i>z</i>-values, showing the consequences for risk assessment and surrogate selection. Along with a review of methods used by scientists, the problem of different methods for calculating the decimal reduction time is highlighted, as well as the lack of specifications for the use of <i>z</i>-values. Additionally, this review proposes the use of the glass capillary method for testing bacterial thermal resistance in liquid foods, such as fruit juices and nectars, in order to standardize the methodology and facilitate comparisons across the literature.</p>","PeriodicalId":155,"journal":{"name":"Comprehensive Reviews in Food Science and Food Safety","volume":"25 1","pages":""},"PeriodicalIF":14.1,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145646964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}