Pub Date : 2024-11-05DOI: 10.1016/j.jfca.2024.106931
Xu Zhang , Xiaoqian Zhan , Wenyu Liu , Ting Wang , Ting Zhang , Changqing Wei
Walnut oil, loaded with unsaturated fatty acids, offers significant health advantages yet is greatly vulnerable to oxidative spoilage.The investigation explored the impact of four oil extraction techniques on the inherent antioxidants, fatty acid profiles, antioxidant efficacy, and oxidation stability index (OSI) of walnut oil. The investigation utilized variance analysis coefficients and partial least squares regression analyses were conducted. It identified β-sitosterol and stigmasterol as crucial inherent antioxidant compounds. The maximal concentrations achieved through hot pressing were 943.16 mg/kg for β-sitosterol and 121.54 mg/kg for stigmasterol. Stigmasterol contribution to the DPPH radical scavenging activity was found to be 0.8224 in PLS1 (R2>0.95). Consequently, the combined effect of these antioxidants on the OSI was tested., revealing that a 2:1 ratio of these substances, under conditions of 45 ℃ ultrasound temperature and 27 minutes ultrasound duration(p<0.05), improved the OSI from 2 to 6.42.
{"title":"Prolonging the oxidative stability of walnut oil by endogenous antioxidants: Phytosterol compounding for improved antioxidant capacity","authors":"Xu Zhang , Xiaoqian Zhan , Wenyu Liu , Ting Wang , Ting Zhang , Changqing Wei","doi":"10.1016/j.jfca.2024.106931","DOIUrl":"10.1016/j.jfca.2024.106931","url":null,"abstract":"<div><div>Walnut oil, loaded with unsaturated fatty acids, offers significant health advantages yet is greatly vulnerable to oxidative spoilage.The investigation explored the impact of four oil extraction techniques on the inherent antioxidants, fatty acid profiles, antioxidant efficacy, and oxidation stability index (OSI) of walnut oil. The investigation utilized variance analysis coefficients and partial least squares regression analyses were conducted. It identified <em>β</em>-sitosterol and stigmasterol as crucial inherent antioxidant compounds. The maximal concentrations achieved through hot pressing were 943.16 mg/kg for <em>β</em>-sitosterol and 121.54 mg/kg for stigmasterol. Stigmasterol contribution to the DPPH radical scavenging activity was found to be 0.8224 in PLS1 (R<sup>2</sup>>0.95). Consequently, the combined effect of these antioxidants on the OSI was tested., revealing that a 2:1 ratio of these substances, under conditions of 45 ℃ ultrasound temperature and 27 minutes ultrasound duration(<em>p</em><0.05), im<em>p</em>roved the OSI from 2 to 6.42.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106931"},"PeriodicalIF":4.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-04DOI: 10.1016/j.jfca.2024.106930
Xinjun Hu , Mingkui Dai , Jianheng Peng , Jiahao Zeng , Jianping Tian , Manjiao Chen
Sorghum, as the primary raw material for brewing, has varieties that are crucial to the quality and yield of the brewing process. To accurately identify and classify different sorghum varieties, a Two-Dimensional Feature Adaptive Convolution Model (DD-FACM) based on data acquired by Hyperspectral imaging (HSI) and 3D Super-Depth-of-Field Microscopy was built. The experimental results demonstrated that the DD-FACM that was built using the combined spectral data and super-depth-of-field image data achieved 100 % accuracy in the identification of 5 varieties of sorghum grains, which was 8 %, 4.2 %, and 4.1 % higher than the classification accuracies of the support vector machine (SVM) model that was built based only the spectral data, the EfficientNet_B3 model built using only the depth-of-field image data, and the DD-FACM built using the combination of the HSI(spectral and RGB image) data, respectively. To verify the effectiveness of the DD-FACM's feature extraction, the extracted features were visualized using t-distributed stochastic neighbor embedding (t-SNE). The results indicated that the DD-FACM based on the spectral data and the image data could achieve the rapid, accurate, and non-destructive identification of different sorghum varieties. This study not only provides brewing enterprises with an efficient method for sorghum variety identification but also offers technical support for variety identification research in related fields.
{"title":"Rapid sorghum variety identification by hyperspectral imaging combined with super-depth-of-field microscopy","authors":"Xinjun Hu , Mingkui Dai , Jianheng Peng , Jiahao Zeng , Jianping Tian , Manjiao Chen","doi":"10.1016/j.jfca.2024.106930","DOIUrl":"10.1016/j.jfca.2024.106930","url":null,"abstract":"<div><div>Sorghum, as the primary raw material for brewing, has varieties that are crucial to the quality and yield of the brewing process. To accurately identify and classify different sorghum varieties, a Two-Dimensional Feature Adaptive Convolution Model (DD-FACM) based on data acquired by Hyperspectral imaging (HSI) and 3D Super-Depth-of-Field Microscopy was built. The experimental results demonstrated that the DD-FACM that was built using the combined spectral data and super-depth-of-field image data achieved 100 % accuracy in the identification of 5 varieties of sorghum grains, which was 8 %, 4.2 %, and 4.1 % higher than the classification accuracies of the support vector machine (SVM) model that was built based only the spectral data, the EfficientNet_B3 model built using only the depth-of-field image data, and the DD-FACM built using the combination of the HSI(spectral and RGB image) data, respectively. To verify the effectiveness of the DD-FACM's feature extraction, the extracted features were visualized using t-distributed stochastic neighbor embedding (t-SNE). The results indicated that the DD-FACM based on the spectral data and the image data could achieve the rapid, accurate, and non-destructive identification of different sorghum varieties. This study not only provides brewing enterprises with an efficient method for sorghum variety identification but also offers technical support for variety identification research in related fields.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106930"},"PeriodicalIF":4.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-04DOI: 10.1016/j.jfca.2024.106926
Ting Shi , Gangcheng Wu , Qingzhe Jin , Xingguo Wang
Camellia oil has become one of the most popular edible vegetable oils especially in China. It can be obtained by cold-pressing (CPE), soxhlet extraction (SE), aqueous enzymatic extraction (AEE), and supercritical carbon dioxide extraction (SC-CO2), while research on their efficient identification is limited. Thus, in this study, proton nuclear magnetic resonance (1H NMR) and conventional chemical analysis, respectively coupled to chemometrics, were employed to compare the camellia oils, produced by CPE, SE, AEE, and SC-CO2. The results showed an obviously overlapping among those four different extracted camellia oils, in both principal component analysis (PCA) and hierarchical clustering analysis (HCA), when using fatty acids as input variables. While two obtained PCA models showed good discrimination, according to the minor component compositions (α-tocopherol, squalene, stigmasterol, β-sitosterol, β-amyrin and lanosterol) and 1H NMR spectra, respectively. Additionally, by means of variable importance for the projection (VIP) scores, less 10 dominant 1H NMR spectra signals were screened out as detailed markers for different camellia oils classification. Therefore, 1H NMR combined with chemometrics may be applied as an efficient technique to classify different extracted camellia oils and potentially other vegetable oils.
{"title":"1H NMR and chemical analysis to characterize camellia oil obtained by different extraction methods: A comparative study using chemometrics","authors":"Ting Shi , Gangcheng Wu , Qingzhe Jin , Xingguo Wang","doi":"10.1016/j.jfca.2024.106926","DOIUrl":"10.1016/j.jfca.2024.106926","url":null,"abstract":"<div><div>Camellia oil has become one of the most popular edible vegetable oils especially in China. It can be obtained by cold-pressing (CPE), soxhlet extraction (SE), aqueous enzymatic extraction (AEE), and supercritical carbon dioxide extraction (SC-CO<sub>2</sub>), while research on their efficient identification is limited. Thus, in this study, proton nuclear magnetic resonance (<sup>1</sup>H NMR) and conventional chemical analysis, respectively coupled to chemometrics, were employed to compare the camellia oils, produced by CPE, SE, AEE, and SC-CO<sub>2</sub>. The results showed an obviously overlapping among those four different extracted camellia oils, in both principal component analysis (PCA) and hierarchical clustering analysis (HCA), when using fatty acids as input variables. While two obtained PCA models showed good discrimination, according to the minor component compositions (α-tocopherol, squalene, stigmasterol, β-sitosterol, β-amyrin and lanosterol) and <sup>1</sup>H NMR spectra, respectively. Additionally, by means of variable importance for the projection (VIP) scores, less 10 dominant <sup>1</sup>H NMR spectra signals were screened out as detailed markers for different camellia oils classification. Therefore, <sup>1</sup>H NMR combined with chemometrics may be applied as an efficient technique to classify different extracted camellia oils and potentially other vegetable oils.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106926"},"PeriodicalIF":4.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research paper presents a novel methodology that integrates electrochemical techniques, specifically electromembrane extraction (EME) and vortex-assisted switchable hydrophilicity solvent-based liquid-phase microextraction (VASHS-LPME). Electrochemical analysis allows for the sensitive detection of Hg(II). The study investigated the impact of vortexing during the switch of the extraction solvent for the first time. Additionally, it explored the potential of vortexing as an alternative to time-consuming temperature control processes, the use of hazardous acids like HCl, and dry ice. The study examined the utilization of potato dextrose agar (PDA) gel as a membrane in EME, eliminating the need for an organic solvent in Hg(II) extraction. We optimized the experimental conditions and found that by meeting specific parameters, we could efficiently extract Hg(II) from a 20 mL solution sample using a PDA gel-based membrane in an aqueous acceptor phase. Subsequently, the VASHS-LPME method was applied under optimal conditions for the extraction of Hg(II) ions. The study demonstrated a linear range of 0.0075–100 μmol L−1, with a limit of quantification (LOQ) and limit of detection (LOD) ranging from 0.0075 to 0.0022 μmol L−1. The precision values obtained for intra- and inter-day relative standard deviations (RSDs) were 3.8 % and 4.3 %, respectively. The technique proposed in the research was effectively employed to determine the quantity of Hg(II) in food and water samples.
{"title":"Electrochemical determination of Hg(II) in aqueous samples using electromembrane extraction combined with vortex-assisted switchable solvent","authors":"Siamak Kiani Shahvandi , Mehrorang Ghaedi , Hamid Ahmar , Pouya Karimi , Hamedreza Javadian","doi":"10.1016/j.jfca.2024.106876","DOIUrl":"10.1016/j.jfca.2024.106876","url":null,"abstract":"<div><div>This research paper presents a novel methodology that integrates electrochemical techniques, specifically electromembrane extraction (EME) and vortex-assisted switchable hydrophilicity solvent-based liquid-phase microextraction (VASHS-LPME). Electrochemical analysis allows for the sensitive detection of Hg(II). The study investigated the impact of vortexing during the switch of the extraction solvent for the first time. Additionally, it explored the potential of vortexing as an alternative to time-consuming temperature control processes, the use of hazardous acids like HCl, and dry ice. The study examined the utilization of potato dextrose agar (PDA) gel as a membrane in EME, eliminating the need for an organic solvent in Hg(II) extraction. We optimized the experimental conditions and found that by meeting specific parameters, we could efficiently extract Hg(II) from a 20 mL solution sample using a PDA gel-based membrane in an aqueous acceptor phase. Subsequently, the VASHS-LPME method was applied under optimal conditions for the extraction of Hg(II) ions. The study demonstrated a linear range of 0.0075–100 μmol L<sup>−1</sup>, with a limit of quantification (LOQ) and limit of detection (LOD) ranging from 0.0075 to 0.0022 μmol L<sup>−1</sup>. The precision values obtained for intra- and inter-day relative standard deviations (RSDs) were 3.8 % and 4.3 %, respectively. The technique proposed in the research was effectively employed to determine the quantity of Hg(II) in food and water samples.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106876"},"PeriodicalIF":4.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.jfca.2024.106928
Dejin Wang , Xingqiang Wu , Jingchun Mao , Zongyi Wang , Yujie Xie , Xudong Wu , Kaixuan Tong , Hongping Man , Chunlin Fan , Hui Chen
In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges (R2> 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.
{"title":"A modified QuEChERS method for the generic and rapid determination of pesticides and mycotoxins in raw coffee beans by liquid chromatography-quadrupole-time-of-flight mass spectrometry","authors":"Dejin Wang , Xingqiang Wu , Jingchun Mao , Zongyi Wang , Yujie Xie , Xudong Wu , Kaixuan Tong , Hongping Man , Chunlin Fan , Hui Chen","doi":"10.1016/j.jfca.2024.106928","DOIUrl":"10.1016/j.jfca.2024.106928","url":null,"abstract":"<div><div>In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges (<em>R</em><sup><em>2</em></sup>> 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106928"},"PeriodicalIF":4.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vibrio parahaemolyticus in seafood is associated with gastroenteritis diseases worldwide. Rapid and sensitive detection methods of the pathogenic strain are essential for monitoring and preventing the diseases. Colorimetric multiplex loop-mediated isothermal amplification combined with lateral flow dipstick assay (mLAMP-LFD) was developed to differentiate pathogenic strains from non-pathogenic V. parahaeholyticus. Thermolabile hemolysin (TLH) and thermo-stable direct hemolysin (TDH) genes were designed and used for mLAMP-LFD assay. The assay specificity was validated using strains belonging to 11 species of other foodborne pathogens, while the sensitivity was evaluated by using a DNA template ranging from 0.005 to 0.4 ng/reaction. Shrimp samples inoculated at 101, 102, and 103 CFU/ 10 g, and enrichment times of 0, 2, 4, 6, and 8 h were used for assay validation. The standardized assay accurately identified 20 V. parahaemolyticus strains and showed no cross-amplification. The assay’s limit of detection was 0.02 ng and took 30 min to complete. Shrimp samples inoculated at 10 CFU of V. parahaemolyticus could be detected following a four-hour enrichment. In conclusion, the high sensitivity and specificity of the mLAMP-LFD assay developed in the present study could be used to identify pathogenic strains of V. parahaemolyticus.
{"title":"Development of colorimetric multiplex loop mediated isothermal amplification combined with lateral flow dipstick (mLAMP-LFD) assay for the identification of TDH toxin-producing Vibrio parahaemolyticus","authors":"Soottawat Benjakul , Phutthipong Sukkapat , Suriya Palamae , Prashant Singh , Mingkwan Yingkajorn , Jirayu Buatong , Jirakrit Saetang","doi":"10.1016/j.jfca.2024.106924","DOIUrl":"10.1016/j.jfca.2024.106924","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> in seafood is associated with gastroenteritis diseases worldwide. Rapid and sensitive detection methods of the pathogenic strain are essential for monitoring and preventing the diseases. Colorimetric multiplex loop-mediated isothermal amplification combined with lateral flow dipstick assay (mLAMP-LFD) was developed to differentiate pathogenic strains from non-pathogenic <em>V. parahaeholyticus</em>. Thermolabile hemolysin (<em>TLH</em>) and thermo-stable direct hemolysin (<em>TDH</em>) genes were designed and used for mLAMP-LFD assay. The assay specificity was validated using strains belonging to 11 species of other foodborne pathogens, while the sensitivity was evaluated by using a DNA template ranging from 0.005 to 0.4 ng/reaction. Shrimp samples inoculated at 10<sup>1</sup>, 10<sup>2</sup>, and 10<sup>3</sup> CFU/ 10 g, and enrichment times of 0, 2, 4, 6, and 8 h were used for assay validation. The standardized assay accurately identified 20 <em>V. parahaemolyticus</em> strains and showed no cross-amplification. The assay’s limit of detection was 0.02 ng and took 30 min to complete. Shrimp samples inoculated at 10 CFU of <em>V. parahaemolyticus</em> could be detected following a four-hour enrichment. In conclusion, the high sensitivity and specificity of the mLAMP-LFD assay developed in the present study could be used to identify pathogenic strains of <em>V. parahaemolyticus</em>.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106924"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jfca.2024.106923
Wenjiao Wang , Zhiqian Liu , Simone Rochfort
Fatty acid (FA) regio-position is critical information for the structures of both triglycerides and phospholipids in food products. Although lipase/phospholipase hydrolysis combined with TLC separation and GC analysis can be used to determine the overall sn-2 FA composition of triglycerides/phospholipids in food samples, it is much more challenging to pinpoint FA regio-position at the molecular species level. Currently, the most widely used method to obtain such information is based on the relative intensity of fragment ions generated in tandem mass spectrometry. The reliability of this approach was verified in this study using regio-pure phospholipid standards and by cross-validation with the Paternò–Büchi photochemical reaction. We have demonstrated that for food materials with widespread regioisomers, a standard calibration curve from regio-pure standards is needed to determine the proportion of each isomer, whereas fragment ion intensity can only be used to reveal the sn-position of FA in phospholipid molecules devoid of regioisomers.
对于食品中甘油三酯和磷脂的结构来说，脂肪酸（FA）的位置是至关重要的信息。虽然脂肪酶/磷脂酶水解结合 TLC 分离和气相色谱分析可用于确定食品样品中甘油三酯/磷脂的整体 sn-2 FA 组成，但要在分子物种水平上确定 FA 的区域位置则更具挑战性。目前，最广泛使用的获取此类信息的方法是基于串联质谱法产生的碎片离子的相对强度。在本研究中，我们使用了纯磷脂标准品，并通过与 Paternò-Büchi 光化学反应进行交叉验证，验证了这种方法的可靠性。我们的研究表明，对于具有广泛区域异构体的食品原料，需要使用区域纯度标准的标准校准曲线来确定每种异构体的比例，而片段离子强度只能用于揭示没有区域异构体的磷脂分子中 FA 的 sn 位置。
{"title":"Can fragment ion intensity be used for fatty acid sn-position determination of food phospholipids?","authors":"Wenjiao Wang , Zhiqian Liu , Simone Rochfort","doi":"10.1016/j.jfca.2024.106923","DOIUrl":"10.1016/j.jfca.2024.106923","url":null,"abstract":"<div><div>Fatty acid (FA) regio-position is critical information for the structures of both triglycerides and phospholipids in food products. Although lipase/phospholipase hydrolysis combined with TLC separation and GC analysis can be used to determine the overall sn-2 FA composition of triglycerides/phospholipids in food samples, it is much more challenging to pinpoint FA regio-position at the molecular species level. Currently, the most widely used method to obtain such information is based on the relative intensity of fragment ions generated in tandem mass spectrometry. The reliability of this approach was verified in this study using regio-pure phospholipid standards and by cross-validation with the Paternò–Büchi photochemical reaction. We have demonstrated that for food materials with widespread regioisomers, a standard calibration curve from regio-pure standards is needed to determine the proportion of each isomer, whereas fragment ion intensity can only be used to reveal the sn-position of FA in phospholipid molecules devoid of regioisomers.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106923"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/j.jfca.2024.106920
Maria Kochetkova, Irina Timofeeva, Daria Frolova, Andrey Bulatov
Ascorbic acid is essential for human health due to its potent antioxidant properties; however, excessive intake can lead to adverse effects. This has created a high demand for simple and cost-effective analytical techniques to determine this analyte outside of laboratory settings for food quality control. In this study, a portable colorimetric sensor was developed for the quantitative on-site determination of ascorbic acid. The technique is based on the reduction of phosphorus heteropoly acid by ascorbic acid, resulting in the formation of a blue-colored product. The heteropoly acid was immobilized on a polymer template in the sensor's indicator zone with menthol used as an environmentally friendly extractant to enhance the device’s stability and extend its shelf life. The colorimetric sensor demonstrated reliable detection with a low limit of detection of 5 mg kg−1, calculated based on a blank test using 3σ. Under optimized conditions, the linear detection range was 15–150 mg kg−1. The disposable sensors were applied to determine ascorbic acid in fruit and vegetable juices and purees for baby food, with recovery rates between 88 % and 110 %. This easy-to-produce and ready-to-use technique presents significant potential for mass on-site screening of ascorbic acid in food samples.
{"title":"Low-cost digital colorimetric sensor for rapid on-site determination of ascorbic acid in vegetable- and fruit-based purees and juices","authors":"Maria Kochetkova, Irina Timofeeva, Daria Frolova, Andrey Bulatov","doi":"10.1016/j.jfca.2024.106920","DOIUrl":"10.1016/j.jfca.2024.106920","url":null,"abstract":"<div><div>Ascorbic acid is essential for human health due to its potent antioxidant properties; however, excessive intake can lead to adverse effects. This has created a high demand for simple and cost-effective analytical techniques to determine this analyte outside of laboratory settings for food quality control. In this study, a portable colorimetric sensor was developed for the quantitative <em>on-site</em> determination of ascorbic acid. The technique is based on the reduction of phosphorus heteropoly acid by ascorbic acid, resulting in the formation of a blue-colored product. The heteropoly acid was immobilized on a polymer template in the sensor's indicator zone with menthol used as an environmentally friendly extractant to enhance the device’s stability and extend its shelf life. The colorimetric sensor demonstrated reliable detection with a low limit of detection of 5 mg kg<sup>−1</sup>, calculated based on a blank test using 3σ. Under optimized conditions, the linear detection range was 15–150 mg kg<sup>−1</sup>. The disposable sensors were applied to determine ascorbic acid in fruit and vegetable juices and purees for baby food, with recovery rates between 88 % and 110 %. This easy-to-produce and ready-to-use technique presents significant potential for mass <em>on-site</em> screening of ascorbic acid in food samples.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106920"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.1016/j.jfca.2024.106912
Luca Garcia, François Garcia, Cédric Saucier
A Syrah red wine ageing experiment was set up during 24 months to measure the influence of different micro-agglomerated corks on wine composition and color. Two new wine ageing indices based on targeting phenolic composition (Iox, oxidation index and Isu, sulfonation index) or colorimetric one (Hue) are proposed. The results showed that the slopes of evolution of these indices varied according to the wine as well as the index studied, and that the initial composition played a predominant role in these evolutions. In addition, the oxygen transfer rate (OTR) of the cork modulates the rate of evolution of the oxidation index and color, but had no impact on the sulfonation index. Partial least squares (PLS) regressions were used in order to establish some models that can predict the ageing index slope based on accelerated ageing tests (AATs), voltammetric parameters and phenolic composition measured before bottling. The models involved different explaining variables for each test and showed good predictive capacities with correlation coefficients (R²) > 0.79.
{"title":"Measuring and modelling Syrah red wines evolution: Ageing tests, color and new molecular indices","authors":"Luca Garcia, François Garcia, Cédric Saucier","doi":"10.1016/j.jfca.2024.106912","DOIUrl":"10.1016/j.jfca.2024.106912","url":null,"abstract":"<div><div>A Syrah red wine ageing experiment was set up during 24 months to measure the influence of different micro-agglomerated corks on wine composition and color. Two new wine ageing indices based on targeting phenolic composition (I<sub>ox</sub>, oxidation index and I<sub>su</sub>, sulfonation index) or colorimetric one (Hue) are proposed. The results showed that the slopes of evolution of these indices varied according to the wine as well as the index studied, and that the initial composition played a predominant role in these evolutions. In addition, the oxygen transfer rate (OTR) of the cork modulates the rate of evolution of the oxidation index and color, but had no impact on the sulfonation index. Partial least squares (PLS) regressions were used in order to establish some models that can predict the ageing index slope based on accelerated ageing tests (AATs), voltammetric parameters and phenolic composition measured before bottling. The models involved different explaining variables for each test and showed good predictive capacities with correlation coefficients (R²) > 0.79.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106912"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a new automated method for the simultaneous determination of mercury and copper in edible oils using deep eutectic solvents and inductively coupled plasma optical emission spectrometry was developed and tested. The developed method uses a deep eutectic solvent consisting of choline chloride, thiourea and lactic acid to effectively extract mercury and copper from edible oil matrices. The extraction process involves automatically passing an oil sample through a microcolumn with the deep eutectic solvent retained as a stationary phase on glass fiber. This approach significantly reduces sample preparation time and improves reproducibility. The established LODs were 0.13 μg L⁻¹ for mercury and 0.12 μg L⁻¹ for copper, while the LOQs were 0.4 μg L⁻¹ and 0.36 μg L⁻¹, respectively. The method demonstrated high accuracy, with relative standard deviations for repeatability and inter-day reproducibility ranging from 2 % to 9 %. The extraction recovery of both metals exceeded 95 %, confirming the efficiency of the DES-based extraction process. An environmental assessment of the method using the AGREEprep tool showed a favorable environmental index of 0.63.
{"title":"Automated simultaneous determination of mercury and copper in edible oils by reversed-phase column-based extraction with deep eutectic solvent","authors":"Andrey Shishov , Sergey Savinov , Nipu Kumar Das , Tamal Banerjee , Andrey Bulatov","doi":"10.1016/j.jfca.2024.106919","DOIUrl":"10.1016/j.jfca.2024.106919","url":null,"abstract":"<div><div>In this study, a new automated method for the simultaneous determination of mercury and copper in edible oils using deep eutectic solvents and inductively coupled plasma optical emission spectrometry was developed and tested. The developed method uses a deep eutectic solvent consisting of choline chloride, thiourea and lactic acid to effectively extract mercury and copper from edible oil matrices. The extraction process involves automatically passing an oil sample through a microcolumn with the deep eutectic solvent retained as a stationary phase on glass fiber. This approach significantly reduces sample preparation time and improves reproducibility. The established LODs were 0.13 μg L⁻¹ for mercury and 0.12 μg L⁻¹ for copper, while the LOQs were 0.4 μg L⁻¹ and 0.36 μg L⁻¹, respectively. The method demonstrated high accuracy, with relative standard deviations for repeatability and inter-day reproducibility ranging from 2 % to 9 %. The extraction recovery of both metals exceeded 95 %, confirming the efficiency of the DES-based extraction process. An environmental assessment of the method using the AGREEprep tool showed a favorable environmental index of 0.63.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106919"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号