首页 > 最新文献

Journal of Food Composition and Analysis最新文献

英文 中文
Design and application of magnetic deep eutectic solvent-based spontaneous liquid-liquid microextraction for the determination of triazole fungicides in water, juice, and wine 设计和应用基于磁性深共晶溶剂的自发液液微萃取法测定水、果汁和葡萄酒中的三唑类杀菌剂
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-25 DOI: 10.1016/j.jfca.2024.106796
In this study, a simple, rapid, and eco-friendly technique, magnetic deep eutectic solvent-based spontaneous liquid-liquid microextraction (MDES-SLLME), was developed to detect triazole fungicides in water, juice, and wine by UHPLC. The unreported SLLME involves injecting the extractant in the whole liquid-phase microextraction, shortening the extraction and separation time without device assistance. MDES produced by heating bio-derived solvent anisole and metal chloride ferric chloride was used as the extractant to reduce the consumption of toxic solvents. Based on the properties of anisole, MDES spontaneously formed small droplets in samples to complete extraction. Based on the properties of ferric chloride, MDES achieved magnetic separation under an external magnetic field. The method was applied to three matrices (water, juice, and wine). The concentration range was 0.007–0.7 mg L−1, and the matrix effect range was 87.4 %–94.9 %. The limit of detection was 0.002 mg L−1. The spiked recoveries ranged from 85.6 % to 94.7 %. The relative standard deviation ranged from 1.7 %–6.3 %. The SLLME requires attention as a potential complement to single-drop microextraction, hollow fiber liquid-phase microextraction, dispersive liquid-liquid microextraction, homogeneous liquid-liquid microextraction, and emulsive liquid-liquid microextraction.
本研究开发了一种简单、快速、环保的技术--基于磁性深共晶溶剂的自发液-液微萃取(MDES-SLLME),利用超高效液相色谱法检测水、果汁和葡萄酒中的三唑类杀菌剂。这种尚未报道的自发液液微萃取方法是在整个液相微萃取过程中注入萃取剂,无需设备辅助即可缩短萃取和分离时间。使用生物衍生溶剂苯甲醚和金属氯化铁加热产生的 MDES 作为萃取剂,以减少有毒溶剂的消耗。根据苯甲醚的特性,MDES 会在样品中自发形成小液滴,从而完成萃取。基于氯化铁的特性,MDES 在外加磁场下实现了磁性分离。该方法适用于三种基质(水、果汁和葡萄酒)。浓度范围为 0.007-0.7 mg L-1,基质效应范围为 87.4%-94.9%。检测限为 0.002 毫克/升。加标回收率为 85.6 % 至 94.7 %。相对标准偏差为 1.7 %-6.3 %。作为对单滴微萃取、中空纤维液相微萃取、分散液液微萃取、均相液液微萃取和乳化液液微萃取的潜在补充,SLLME 值得关注。
{"title":"Design and application of magnetic deep eutectic solvent-based spontaneous liquid-liquid microextraction for the determination of triazole fungicides in water, juice, and wine","authors":"","doi":"10.1016/j.jfca.2024.106796","DOIUrl":"10.1016/j.jfca.2024.106796","url":null,"abstract":"<div><div>In this study, a simple, rapid, and eco-friendly technique, magnetic deep eutectic solvent-based spontaneous liquid-liquid microextraction (MDES-SLLME), was developed to detect triazole fungicides in water, juice, and wine by UHPLC. The unreported SLLME involves injecting the extractant in the whole liquid-phase microextraction, shortening the extraction and separation time without device assistance. MDES produced by heating bio-derived solvent anisole and metal chloride ferric chloride was used as the extractant to reduce the consumption of toxic solvents. Based on the properties of anisole, MDES spontaneously formed small droplets in samples to complete extraction. Based on the properties of ferric chloride, MDES achieved magnetic separation under an external magnetic field. The method was applied to three matrices (water, juice, and wine). The concentration range was 0.007–0.7 mg L<sup>−1</sup>, and the matrix effect range was 87.4 %–94.9 %. The limit of detection was 0.002 mg L<sup>−1</sup>. The spiked recoveries ranged from 85.6 % to 94.7 %. The relative standard deviation ranged from 1.7 %–6.3 %. The SLLME requires attention as a potential complement to single-drop microextraction, hollow fiber liquid-phase microextraction, dispersive liquid-liquid microextraction, homogeneous liquid-liquid microextraction, and emulsive liquid-liquid microextraction.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of moisture and size of winter melon seeds based on hyperspectral imaging and convex polygon size measurement 基于高光谱成像和凸多边形尺寸测量的冬瓜种子水分和尺寸检测
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-25 DOI: 10.1016/j.jfca.2024.106789
<div><div>The level of moisture content and size of winter melon seeds affect their storage, germination and growth processes. Moderate moisture and size are essential for seed germination and growth. Therefore, detecting the moisture and size of winter melon seeds is beneficial to improve their germination rate. Traditional seed moisture testing methods are complex to operate and require destructive sample preparation or chemical treatment. Common dimensional measurement methods are also time-consuming and laborious. Hyperspectral imaging technology can acquire information about the surface and internal structure of the target separately, it can be used to quickly and non-destructively detect the moisture and size of winter melon seeds. In this study, partial least squares regression (PLSR) and partial least squares support vector machine (LSSVM) models were established to predict the moisture content of winter melon seeds by using reflection and transmittance spectral data. The models were optimized using five variable selection methods. The optimal performance of the LSSVM model based on the CARS algorithm was achieved in both single reflection and transmission spectra. The <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> and RMSEP of the model based on the reflection spectra were 0.9667 % and 0.0215 %, respectively. The <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> and RMSEP of the model based on the transmission spectra were 0.9644 % and 0.0223 %, respectively. In low-level data fusion, the LSSVM model based on the CARS algorithm also achieved optimal performance, but with only a little improvement compared to a single model (reflection spectra or transmission spectra), with <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> and RMSEP of 0.9679 % and 0.0212 %, respectively. In the mid-level data fusion, the LSSVM model also based on the CARS algorithm achieved the optimal performance, and the performance of the model was further improved. The <span><math><msubsup><mrow><mi>R</mi></mrow><mrow><mi>P</mi></mrow><mrow><mn>2</mn></mrow></msubsup></math></span> and RMSEP of the model were 0.9738 % and 0.0191 %, respectively. Finally, the image processing algorithm and the convex polygon size measurement method was proposed to measure the size of winter melon seeds. The absolute error between the calibrated winter melon seed length and width and the true length and width was less than 0.22 mm, and the relative error was less than 2 %. The results show that hyperspectral imaging technology can accurately detect the water content of winter melon seeds. Data fusion method and LSSVM model based on CARS algorithm can detect the water content of winter melon seeds more accurately. The image processing algorithm combined with the convex polygon size measuremen
冬瓜种子的含水量和大小会影响其储藏、发芽和生长过程。适度的水分和大小对种子的发芽和生长至关重要。因此,检测冬瓜种子的水分和大小有利于提高其发芽率。传统的种子水分检测方法操作复杂,需要进行破坏性的样品制备或化学处理。普通的尺寸测量方法也费时费力。高光谱成像技术可分别获取目标物的表面和内部结构信息,可用于快速、无损地检测冬瓜种子的水分和尺寸。本研究建立了偏最小二乘回归(PLSR)和偏最小二乘支持向量机(LSSVM)模型,利用反射和透射光谱数据预测冬瓜子的水分含量。使用五种变量选择方法对模型进行了优化。基于 CARS 算法的 LSSVM 模型在单反射光谱和透射光谱中都达到了最佳性能。基于反射光谱的模型的 RP2 和 RMSEP 分别为 0.9667 % 和 0.0215 %。基于透射光谱的模型的 RP2 和 RMSEP 分别为 0.9644 % 和 0.0223 %。在低层次数据融合中,基于 CARS 算法的 LSSVM 模型也达到了最佳性能,但与单一模型(反射光谱或透射光谱)相比仅有些许改进,RP2 和 RMSEP 分别为 0.9679 % 和 0.0212 %。在中层数据融合中,同样基于 CARS 算法的 LSSVM 模型达到了最优性能,模型的性能得到了进一步提高。模型的 RP2 和 RMSEP 分别为 0.9738 % 和 0.0191 %。最后,提出了图像处理算法和凸多边形尺寸测量方法来测量冬瓜种子的尺寸。标定的冬瓜种子长宽与真实长宽的绝对误差小于 0.22 毫米,相对误差小于 2%。结果表明,高光谱成像技术可以准确检测冬瓜种子的含水量。基于 CARS 算法的数据融合方法和 LSSVM 模型能更准确地检测冬瓜种子的含水量。图像处理算法结合凸多边形尺寸测量方法可有效地用于高精度检测冬瓜种子的尺寸。
{"title":"Detection of moisture and size of winter melon seeds based on hyperspectral imaging and convex polygon size measurement","authors":"","doi":"10.1016/j.jfca.2024.106789","DOIUrl":"10.1016/j.jfca.2024.106789","url":null,"abstract":"&lt;div&gt;&lt;div&gt;The level of moisture content and size of winter melon seeds affect their storage, germination and growth processes. Moderate moisture and size are essential for seed germination and growth. Therefore, detecting the moisture and size of winter melon seeds is beneficial to improve their germination rate. Traditional seed moisture testing methods are complex to operate and require destructive sample preparation or chemical treatment. Common dimensional measurement methods are also time-consuming and laborious. Hyperspectral imaging technology can acquire information about the surface and internal structure of the target separately, it can be used to quickly and non-destructively detect the moisture and size of winter melon seeds. In this study, partial least squares regression (PLSR) and partial least squares support vector machine (LSSVM) models were established to predict the moisture content of winter melon seeds by using reflection and transmittance spectral data. The models were optimized using five variable selection methods. The optimal performance of the LSSVM model based on the CARS algorithm was achieved in both single reflection and transmission spectra. The &lt;span&gt;&lt;math&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;/msubsup&gt;&lt;/math&gt;&lt;/span&gt; and RMSEP of the model based on the reflection spectra were 0.9667 % and 0.0215 %, respectively. The &lt;span&gt;&lt;math&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;/msubsup&gt;&lt;/math&gt;&lt;/span&gt; and RMSEP of the model based on the transmission spectra were 0.9644 % and 0.0223 %, respectively. In low-level data fusion, the LSSVM model based on the CARS algorithm also achieved optimal performance, but with only a little improvement compared to a single model (reflection spectra or transmission spectra), with &lt;span&gt;&lt;math&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;/msubsup&gt;&lt;/math&gt;&lt;/span&gt; and RMSEP of 0.9679 % and 0.0212 %, respectively. In the mid-level data fusion, the LSSVM model also based on the CARS algorithm achieved the optimal performance, and the performance of the model was further improved. The &lt;span&gt;&lt;math&gt;&lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;R&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow&gt;&lt;mn&gt;2&lt;/mn&gt;&lt;/mrow&gt;&lt;/msubsup&gt;&lt;/math&gt;&lt;/span&gt; and RMSEP of the model were 0.9738 % and 0.0191 %, respectively. Finally, the image processing algorithm and the convex polygon size measurement method was proposed to measure the size of winter melon seeds. The absolute error between the calibrated winter melon seed length and width and the true length and width was less than 0.22 mm, and the relative error was less than 2 %. The results show that hyperspectral imaging technology can accurately detect the water content of winter melon seeds. Data fusion method and LSSVM model based on CARS algorithm can detect the water content of winter melon seeds more accurately. The image processing algorithm combined with the convex polygon size measuremen","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Real-time monitoring of cattle (Bos taurus) tissue using a novel point-of-care (POC) polymerase spiral reaction (PSR) colorimetric assay 使用新型护理点(POC)聚合酶螺旋反应(PSR)比色法实时监测牛(金牛)组织
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.jfca.2024.106773
Adulteration of cattle meat with other species raises critical public health concerns, as existing detection methods lack sensitivity, specificity and practicality. We address this gap by introducing a novel Point-of-Care (POC) polymerase spiral reaction (PSR) assay. This rapid, on-site tool enables highly specific and sensitive identification of cattle tissue, even in resource-limited settings. This innovation paves the way for improved food safety and consumer protection. The cattle-specific PSR assay employs COX3 gene-targeting primers and a chromogenic indicator triad (HNB, Malachite Green and Phenol Red) for rapid visual detection. Cross-amplification absence with other meats (buffalo, sheep, goat and pig) and concordance with SYBR Green I and agarose gel electrophoresis confirm specificity. PSR at 62 °C for 60 minutes exhibits a 100 fg detection limit and identifies 0.1 % meat admixing. Efficacy is demonstrated across a diverse range of cattle meat samples, blind samples and processed cattle muscle samples. Compatibility with both conventional (snap chill) and commercial DNA extraction kits enables on-site analysis within 90 minutes, facilitating rapid field sample analysis.
由于现有的检测方法缺乏灵敏度、特异性和实用性,牛肉掺杂其他物种的问题引发了严重的公共卫生问题。为了弥补这一不足,我们推出了一种新型的护理点(POC)聚合酶螺旋反应(PSR)检测方法。即使在资源有限的环境中,这种快速的现场工具也能对牛组织进行高度特异和灵敏的鉴定。这项创新为改善食品安全和消费者保护铺平了道路。牛特异性 PSR 检测法采用 COX3 基因靶向引物和三色显色指示剂(HNB、孔雀石绿和酚红)进行快速肉眼检测。与其他肉类(水牛、绵羊、山羊和猪)的交叉扩增缺失以及与 SYBR Green I 和琼脂糖凝胶电泳的一致性证实了其特异性。62 °C 60 分钟的 PSR 检测限为 100 fg,可识别 0.1 % 的肉类混合物。在各种牛肉样品、盲样品和加工过的牛肌肉样品中都证明了其有效性。与传统(快速冷冻)和商用 DNA 提取试剂盒兼容,可在 90 分钟内完成现场分析,便于快速进行现场样品分析。
{"title":"Real-time monitoring of cattle (Bos taurus) tissue using a novel point-of-care (POC) polymerase spiral reaction (PSR) colorimetric assay","authors":"","doi":"10.1016/j.jfca.2024.106773","DOIUrl":"10.1016/j.jfca.2024.106773","url":null,"abstract":"<div><div>Adulteration of cattle meat with other species raises critical public health concerns, as existing detection methods lack sensitivity, specificity and practicality. We address this gap by introducing a novel Point-of-Care (POC) polymerase spiral reaction (PSR) assay. This rapid, on-site tool enables highly specific and sensitive identification of cattle tissue, even in resource-limited settings. This innovation paves the way for improved food safety and consumer protection. The cattle-specific PSR assay employs <em>COX3</em> gene-targeting primers and a chromogenic indicator triad (HNB, Malachite Green and Phenol Red) for rapid visual detection. Cross-amplification absence with other meats (buffalo, sheep, goat and pig) and concordance with SYBR Green I and agarose gel electrophoresis confirm specificity. PSR at 62 °C for 60 minutes exhibits a 100 fg detection limit and identifies 0.1 % meat admixing. Efficacy is demonstrated across a diverse range of cattle meat samples, blind samples and processed cattle muscle samples. Compatibility with both conventional (snap chill) and commercial DNA extraction kits enables on-site analysis within 90 minutes, facilitating rapid field sample analysis.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multilayer spatial-spectral segmentation network for detecting AFB1 用于检测 AFB1 的多层空间光谱分割网络
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.jfca.2024.106790
Aflatoxin, a toxin produced by Aspergillus flavus, is commonly found in peanuts and poses a significant threat to human health. In this paper, we propose an adjustable Mul-U-Net model, capable of learning both spectral and spatial information in parallel, facilitating rapid detection of aflatoxins by adjusting the number of central spatial U units. First, AFB1 was semantically segmented by inputting hyperspectral image cubes into six semantic segmentation models: Mul-U-Net, U-Net, FCN, SegNet, CNN, and RefineNet. Experimental results demonstrate that Mul-U-Net outperformed the other models, achieving an overall accuracy of 95.64 % and an RMSE of 0.2079. We further examined the influence of different spatial U units on model performance and found that the optimal spatial U count matched the number of labels. In the aflatoxin dataset, the Mul-2 U-Net model achieved the highest accuracy of 96.12 %. Further validation on the Indian Pines and Pavia University datasets showed that Mul-16 U-Net and Mul-9 U-Net achieved the highest accuracies of 98.12 % and 98.01 %, respectively. This study presents a robust, high-performance, and tunable Mul-U-Net semantic segmentation network, offering a valuable new approach for the identification of aflatoxins in peanuts.
黄曲霉毒素是一种由黄曲霉菌产生的毒素,通常存在于花生中,对人类健康构成严重威胁。在本文中,我们提出了一种可调整的 Mul-U-Net 模型,该模型能够同时学习光谱和空间信息,通过调整中心空间 U 单元的数量来促进黄曲霉毒素的快速检测。首先,将高光谱图像立方体输入六个语义分割模型,对 AFB1 进行语义分割:这六种模型分别是:Mul-U-Net、U-Net、FCN、SegNet、CNN 和 RefineNet。实验结果表明,Mul-U-Net 的表现优于其他模型,总体准确率达到 95.64 %,RMSE 为 0.2079。我们进一步研究了不同空间 U 单位对模型性能的影响,发现最佳空间 U 数量与标签数量相匹配。在黄曲霉毒素数据集中,Mul-2 U-Net 模型的准确率最高,达到 96.12%。在印度松树和帕维亚大学数据集上的进一步验证表明,Mul-16 U-Net 和 Mul-9 U-Net 的准确率最高,分别达到 98.12 % 和 98.01 %。本研究提出了一种稳健、高性能和可调整的 Mul-U-Net 语义分割网络,为识别花生中的黄曲霉毒素提供了一种有价值的新方法。
{"title":"Multilayer spatial-spectral segmentation network for detecting AFB1","authors":"","doi":"10.1016/j.jfca.2024.106790","DOIUrl":"10.1016/j.jfca.2024.106790","url":null,"abstract":"<div><div>Aflatoxin, a toxin produced by <em>Aspergillus flavus</em>, is commonly found in peanuts and poses a significant threat to human health. In this paper, we propose an adjustable Mul-U-Net model, capable of learning both spectral and spatial information in parallel, facilitating rapid detection of aflatoxins by adjusting the number of central spatial U units. First, AFB<sub>1</sub> was semantically segmented by inputting hyperspectral image cubes into six semantic segmentation models: Mul-U-Net, U-Net, FCN, SegNet, CNN, and RefineNet. Experimental results demonstrate that Mul-U-Net outperformed the other models, achieving an overall accuracy of 95.64 % and an RMSE of 0.2079. We further examined the influence of different spatial U units on model performance and found that the optimal spatial U count matched the number of labels. In the aflatoxin dataset, the Mul-2 U-Net model achieved the highest accuracy of 96.12 %. Further validation on the Indian Pines and Pavia University datasets showed that Mul-16 U-Net and Mul-9 U-Net achieved the highest accuracies of 98.12 % and 98.01 %, respectively. This study presents a robust, high-performance, and tunable Mul-U-Net semantic segmentation network, offering a valuable new approach for the identification of aflatoxins in peanuts.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemical sensor based on Fe3O4@Au/MOF-P2W17V composite modified glassy carbon electrode for food nitrite detection 基于 Fe3O4@Au/MOF-P2W17V 复合改性玻璃碳电极的电化学传感器用于食品亚硝酸盐检测
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.jfca.2024.106792
Excessive consumption of nitrites can be harmful to human health. Therefore, the design of novel electrocatalysts for efficient electrochemical quantification of nitrite is important. In this paper, a magnetic metal-organic framework (Fe3O4@Au/MOF) was synthesized by a self-assembly method using waste orange peel as the reducing agent, and vanadium-substituted tungsten phosphate (K7P2W17VO62-18H2O, P2W17V) and Fe3O4@Au/MOF were loaded on glassy carbon electrodes for the first time by layer-by-layer self-assembly and electrodeposition. Then, a nitrite sensor based on Fe3O4@Au/MOF-P2W17V nanocomposites was prepared. The composites' characterization reveals that the combination of P2W17V with Fe3O4@Au/MOF not only inhibits nanoparticle agglomeration but also offers high catalytic activity and electrical conductivity when compared to pure Fe3O4@Au/MOF and P2W17V. Under the optimal conditions, the Fe3O4@Au/MOF-P2W17V sensor's linear range for detecting nitrite was 0.01–100 mM, with a sensitivity of 11.682 μA·μM−1·cm−2 and a detection limit as low as 0.532 μM. In 100 cycle stability trials and 30 d reproducibility experiments, the current peak retained more than 95 % of its initial value. Furthermore, the sensor demonstrated good selectivity and has been successfully applied to detect nitrite in ham sausage, squash, milk and brined quail eggs, and the results were basically consistent with those of the naphthylenediamine hydrochloride method. The effective fabrication of Fe3O4@Au/MOF-P2W17V opens up a new avenue for determining low amounts of nitrite in analytical applications that is viable for practical use.
过量摄入亚硝酸盐会对人体健康造成危害。因此,设计新型电催化剂以实现亚硝酸盐的高效电化学定量非常重要。本文以废弃橘皮为还原剂,采用自组装方法合成了磁性金属有机框架(Fe3O4@Au/MOF),并首次通过逐层自组装和电沉积的方法将磷酸钒取代钨(K7P2W17VO62-18H2O,P2W17V)和Fe3O4@Au/MOF负载在玻璃碳电极上。然后,制备了基于 Fe3O4@Au/MOF-P2W17V 纳米复合材料的亚硝酸盐传感器。复合材料的表征结果表明,与纯 Fe3O4@Au/MOF 和 P2W17V 相比,P2W17V 与 Fe3O4@Au/MOF 的结合不仅能抑制纳米颗粒的团聚,还能提供较高的催化活性和导电性。在最佳条件下,Fe3O4@Au/MOF-P2W17V 传感器检测亚硝酸盐的线性范围为 0.01-100 mM,灵敏度为 11.682 μA-μM-1-cm-2,检测限低至 0.532 μM。在 100 个周期的稳定性试验和 30 天的重现性实验中,电流峰值保持了其初始值的 95% 以上。此外,该传感器还具有良好的选择性,已成功应用于火腿肠、地瓜、牛奶和盐渍鹌鹑蛋中亚硝酸盐的检测,结果与盐酸萘二胺法基本一致。Fe3O4@Au/MOF-P2W17V的有效制备为在分析应用中检测低量亚硝酸盐开辟了一条新途径,具有很强的实用性。
{"title":"Electrochemical sensor based on Fe3O4@Au/MOF-P2W17V composite modified glassy carbon electrode for food nitrite detection","authors":"","doi":"10.1016/j.jfca.2024.106792","DOIUrl":"10.1016/j.jfca.2024.106792","url":null,"abstract":"<div><div>Excessive consumption of nitrites can be harmful to human health. Therefore, the design of novel electrocatalysts for efficient electrochemical quantification of nitrite is important. In this paper, a magnetic metal-organic framework (Fe<sub>3</sub>O<sub>4</sub>@Au/MOF) was synthesized by a self-assembly method using waste orange peel as the reducing agent, and vanadium-substituted tungsten phosphate (K<sub>7</sub>P<sub>2</sub>W<sub>17</sub>VO<sub>62</sub>-18H<sub>2</sub>O, P<sub>2</sub>W<sub>17</sub>V) and Fe<sub>3</sub>O<sub>4</sub>@Au/MOF were loaded on glassy carbon electrodes for the first time by layer-by-layer self-assembly and electrodeposition. Then, a nitrite sensor based on Fe<sub>3</sub>O<sub>4</sub>@Au/MOF-P<sub>2</sub>W<sub>17</sub>V nanocomposites was prepared. The composites' characterization reveals that the combination of P<sub>2</sub>W<sub>17</sub>V with Fe<sub>3</sub>O<sub>4</sub>@Au/MOF not only inhibits nanoparticle agglomeration but also offers high catalytic activity and electrical conductivity when compared to pure Fe<sub>3</sub>O<sub>4</sub>@Au/MOF and P<sub>2</sub>W<sub>17</sub>V. Under the optimal conditions, the Fe<sub>3</sub>O<sub>4</sub>@Au/MOF-P<sub>2</sub>W<sub>17</sub>V sensor's linear range for detecting nitrite was 0.01–100 mM, with a sensitivity of 11.682 μA·μM<sup>−1</sup>·cm<sup>−2</sup> and a detection limit as low as 0.532 μM. In 100 cycle stability trials and 30 d reproducibility experiments, the current peak retained more than 95 % of its initial value. Furthermore, the sensor demonstrated good selectivity and has been successfully applied to detect nitrite in ham sausage, squash, milk and brined quail eggs, and the results were basically consistent with those of the naphthylenediamine hydrochloride method. The effective fabrication of Fe<sub>3</sub>O<sub>4</sub>@Au/MOF-P<sub>2</sub>W<sub>17</sub>V opens up a new avenue for determining low amounts of nitrite in analytical applications that is viable for practical use.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel method for rice identification: Coupling Raman spectroscopy with Fourier spectrum and analyzing with deep learning 水稻识别的新方法将拉曼光谱与傅立叶光谱耦合并利用深度学习进行分析
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.jfca.2024.106793
The development of detection methods for food adulteration is of great significance for promoting innovation and development in food safety supervision technology. In this study, we proposed a novel model: Raman-Fourier-BiLSTM-CNN (RFBC), which combines Raman spectroscopy with deep learning to achieve precise identification of seven brands of japonica rice in the northeastern region of China. This model focuses on revealing the worth of the Fourier spectrum of Raman spectra. The raw Raman spectra and their Fourier spectra are cleverly coupled through a Bi-directional Long Short-Term Memory (BiLSTM) connection structure, and the resulting composite features are deeply analyzed by a Convolutional Neural Network (CNN). Compared to traditional algorithms, RFBC exhibits superior feature extraction capabilities. To further evaluate the recognition performance of RFBC, this study compared it with the RFBC model containing only the original Raman spectrum branch, as well as with machine learning models based on Principal Component Analysis (PCA), including Support Vector Machine (SVM), Random Forest (RF), K-Nearest Neighbor (KNN), and eXtreme Gradient Boosting (XGBoost). The results show that, compared with the other five models, RFBC can accurately identify different brands of japonica rice, with significant advantages in accuracy, precision, recall, and other aspects. RFBC achieves a classification accuracy of 97.1 %, macro precision of 97.2 %, macro recall of 97.1 %, and macro F1-score of 97.2 %. The system proposed in this study can more accurately identify brands of japonica rice, providing strong technical support for combating counterfeit japonica rice products.
开发食品掺假检测方法对促进食品安全监管技术的创新和发展具有重要意义。在本研究中,我们提出了一种新型模型:拉曼光谱与深度学习相结合的拉曼-傅立叶-BiLSTM-CNN(Raman-Fourier-BiLSTM-CNN,RFBC)模型,实现了对东北地区七种品牌粳米的精准识别。该模型重点揭示了拉曼光谱傅立叶谱的价值。原始拉曼光谱及其傅立叶光谱通过双向长短期记忆(BiLSTM)连接结构巧妙地耦合在一起,由此产生的复合特征由卷积神经网络(CNN)进行深度分析。与传统算法相比,RFBC 表现出更出色的特征提取能力。为了进一步评估 RFBC 的识别性能,本研究将其与仅包含原始拉曼光谱分支的 RFBC 模型以及基于主成分分析 (PCA) 的机器学习模型进行了比较,包括支持向量机 (SVM)、随机森林 (RF)、K-近邻 (KNN) 和 eXtreme Gradient Boosting (XGBoost)。结果表明,与其他五种模型相比,RFBC 可以准确识别不同品牌的粳米,在准确度、精确度、召回率等方面具有显著优势。RFBC 的分类准确率为 97.1%,宏观准确率为 97.2%,宏观召回率为 97.1%,宏观 F1 分数为 97.2%。本研究提出的系统能更准确地识别粳米品牌,为打击假冒粳米产品提供了有力的技术支持。
{"title":"A novel method for rice identification: Coupling Raman spectroscopy with Fourier spectrum and analyzing with deep learning","authors":"","doi":"10.1016/j.jfca.2024.106793","DOIUrl":"10.1016/j.jfca.2024.106793","url":null,"abstract":"<div><div>The development of detection methods for food adulteration is of great significance for promoting innovation and development in food safety supervision technology. In this study, we proposed a novel model: Raman-Fourier-BiLSTM-CNN (RFBC), which combines Raman spectroscopy with deep learning to achieve precise identification of seven brands of japonica rice in the northeastern region of China. This model focuses on revealing the worth of the Fourier spectrum of Raman spectra. The raw Raman spectra and their Fourier spectra are cleverly coupled through a Bi-directional Long Short-Term Memory (BiLSTM) connection structure, and the resulting composite features are deeply analyzed by a Convolutional Neural Network (CNN). Compared to traditional algorithms, RFBC exhibits superior feature extraction capabilities. To further evaluate the recognition performance of RFBC, this study compared it with the RFBC model containing only the original Raman spectrum branch, as well as with machine learning models based on Principal Component Analysis (PCA), including Support Vector Machine (SVM), Random Forest (RF), K-Nearest Neighbor (KNN), and eXtreme Gradient Boosting (XGBoost). The results show that, compared with the other five models, RFBC can accurately identify different brands of japonica rice, with significant advantages in accuracy, precision, recall, and other aspects. RFBC achieves a classification accuracy of 97.1 %, macro precision of 97.2 %, macro recall of 97.1 %, and macro F1-score of 97.2 %. The system proposed in this study can more accurately identify brands of japonica rice, providing strong technical support for combating counterfeit japonica rice products.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Compliance with voluntary nutritional labelling on alcoholic beverages in Spain 西班牙酒精饮料自愿营养标签的遵守情况
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.jfca.2024.106791
Access to nutritional information is a fundamental consumer´s right, as it facilitates informed decision-making regarding dietary choices. However, excluding a recent directive concerning wine products, European Union regulations grant exemption to beverages with an alcohol content exceeding 1.2 % from the requirement of disclosing nutritional values. The purpose of this study was to evaluate the industry´s compliance with voluntary commitments for nutritional labelling of alcoholic beverages in Spain. A cross-sectional study was conducted in the municipality of Madrid (Spain) during March and April 2023. Data on 627 alcoholic beverages were collected. We considered that a product label displayed complete nutritional data when it showed the energy value per 100 mL as well as the corresponding macronutrients (fats, carbohydrates, and proteins). Only 22.2 % of these products provided any nutritional information, with beers leading at 56.5 %, followed by spirits (26.5 %), vermouths and aperitifs (16.7 %), and wines and sparkling wines significantly fewer at 2.9 %. Only 2.4 % of the sampled beverages provided comprehensive nutritional information including energy and macronutrients. These findings highlight a failure to meet voluntary labelling commitments in Spain, with significant variations observed across sectors. The government should ensure consumers´ access to readily available and accurate information regarding the nutritional composition of alcoholic beverages.
获取营养信息是消费者的一项基本权利,因为这有助于在知情的情况下做出饮食选择。然而,除最近一项有关葡萄酒产品的指令外,欧盟法规对酒精含量超过 1.2%的饮料免除了披露营养价值的要求。本研究旨在评估西班牙酒精饮料行业对营养标签自愿承诺的遵守情况。这项横向研究于 2023 年 3 月至 4 月期间在马德里市(西班牙)进行。收集了 627 种酒精饮料的数据。当产品标签显示每 100 毫升的能量值以及相应的宏量营养素(脂肪、碳水化合物和蛋白质)时,我们认为该产品标签显示了完整的营养数据。这些产品中只有 22.2% 提供了任何营养信息,其中啤酒占 56.5%,其次是烈性酒(26.5%)、苦艾酒和开胃酒(16.7%),而葡萄酒和气泡酒则少得多,仅占 2.9%。只有 2.4%的抽样饮料提供了全面的营养信息,包括能量和宏量营养素。这些调查结果表明,西班牙未能履行自愿性标签承诺,各行业之间的差异很大。政府应确保消费者能够随时获得有关酒精饮料营养成分的准确信息。
{"title":"Compliance with voluntary nutritional labelling on alcoholic beverages in Spain","authors":"","doi":"10.1016/j.jfca.2024.106791","DOIUrl":"10.1016/j.jfca.2024.106791","url":null,"abstract":"<div><div>Access to nutritional information is a fundamental consumer´s right, as it facilitates informed decision-making regarding dietary choices. However, excluding a recent directive concerning wine products, European Union regulations grant exemption to beverages with an alcohol content exceeding 1.2 % from the requirement of disclosing nutritional values. The purpose of this study was to evaluate the industry´s compliance with voluntary commitments for nutritional labelling of alcoholic beverages in Spain. A cross-sectional study was conducted in the municipality of Madrid (Spain) during March and April 2023. Data on 627 alcoholic beverages were collected. We considered that a product label displayed complete nutritional data when it showed the energy value per 100 mL as well as the corresponding macronutrients (fats, carbohydrates, and proteins). Only 22.2 % of these products provided any nutritional information, with beers leading at 56.5 %, followed by spirits (26.5 %), vermouths and aperitifs (16.7 %), and wines and sparkling wines significantly fewer at 2.9 %. Only 2.4 % of the sampled beverages provided comprehensive nutritional information including energy and macronutrients. These findings highlight a failure to meet voluntary labelling commitments in Spain, with significant variations observed across sectors. The government should ensure consumers´ access to readily available and accurate information regarding the nutritional composition of alcoholic beverages.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Removal residual, commercial processing factor and risk assessment of four fungicides in orange fruit and various processing by-products 橙果和各种加工副产品中四种杀菌剂的去除残留量、商业加工因素和风险评估
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-23 DOI: 10.1016/j.jfca.2024.106786
The residual behaviors and processing factors of four fungicides (prochloraz, kasugamycin, oxine-copper and fenaminstrobin) in orange fruit and various processing by-products were evaluated. The residues of kasugamycin, oxine-copper and fenaminstrobin were detected using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with QuEChERS pretreatment method and that of prochloraz was monitored using gas chromatography with an electron capture detector (GC-ECD). The results showed washing processing reduced residues by 8.6 %-33.9 %. Juicing was an effective process to reduce pesticide residues by 65.6 %-98.7 % with processing factor (PF) of 0.021–0.339. However, high levels of residual prochloraz, oxine-copper and fenaminstrobin (from 5.777 to 51.533 mg/kg) were detected in orange essential oils, with PF of 8.720–14.558. Prochloraz was more easily enriched in wet pomace compared with the other three fungicides. When spraying was conducted in accordance with good agricultural practices and using the recommended highest pesticide application dosage and frequency, the dietary intake exposure risks of the four residual fungicides in orange and its processed juices in China were less than 100 % for different age groups and caused no unacceptable risks to human health.
评估了四种杀菌剂(丙环唑、春雷霉素、氧乐果和唑螨酯)在橘子果实和各种加工副产品中的残留行为和加工因素。采用超高效液相色谱-串联质谱(UPLC-MS/MS)结合 QuEChERS 预处理方法检测了春雷霉素、氧乐果和唑菌酰胺的残留量,采用气相色谱-电子捕获检测器(GC-ECD)监测了丙草胺的残留量。结果表明,清洗处理可减少 8.6 %-33.9 % 的残留物。榨汁是一种有效的加工方法,可减少 65.6 %-98.7 % 的农药残留,加工系数(PF)为 0.021-0.339。然而,在橙子精油中检测到了高浓度的残留丙草胺、氧氯化铜和杀螟丹(从 5.777 到 51.533 毫克/千克不等),加工系数为 8.720-14.558 。与其他三种杀菌剂相比,丙草胺更容易在湿果渣中富集。如果按照良好农业规范进行喷洒,并采用建议的最高农药施用剂量和频率,中国不同年龄组的橘子及其加工果汁中四种残留杀菌剂的膳食摄入暴露风险均小于 100%,不会对人体健康造成不可接受的风险。
{"title":"Removal residual, commercial processing factor and risk assessment of four fungicides in orange fruit and various processing by-products","authors":"","doi":"10.1016/j.jfca.2024.106786","DOIUrl":"10.1016/j.jfca.2024.106786","url":null,"abstract":"<div><div>The residual behaviors and processing factors of four fungicides (prochloraz, kasugamycin, oxine-copper and fenaminstrobin) in orange fruit and various processing by-products were evaluated. The residues of kasugamycin, oxine-copper and fenaminstrobin were detected using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with QuEChERS pretreatment method and that of prochloraz was monitored using gas chromatography with an electron capture detector (GC-ECD). The results showed washing processing reduced residues by 8.6 %-33.9 %. Juicing was an effective process to reduce pesticide residues by 65.6 %-98.7 % with processing factor (<em>PF</em>) of 0.021–0.339. However, high levels of residual prochloraz, oxine-copper and fenaminstrobin (from 5.777 to 51.533 mg/kg) were detected in orange essential oils, with <em>PF</em> of 8.720–14.558. Prochloraz was more easily enriched in wet pomace compared with the other three fungicides. When spraying was conducted in accordance with good agricultural practices and using the recommended highest pesticide application dosage and frequency, the dietary intake exposure risks of the four residual fungicides in orange and its processed juices in China were less than 100 % for different age groups and caused no unacceptable risks to human health.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct quadruplex real-time VPCR for simultaneous rapid detection of Pinelliae Rhizoma, Rhizoma Pinelliae Pedatisectae and Rhizoma Typhonii Flagelliformis 直接四重实时 VPCR,用于同时快速检测赤松菌、赤松 Pedatisectae 菌和韧皮部鞭毛菌
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-23 DOI: 10.1016/j.jfca.2024.106761
Known for its health benefits, Pinelliae Rhizoma is frequently utilized in many botanical dietary supplements and herbal products. However, the substantial demand has led to the frequent adulteration with morphologically similar species, such as Rhizoma Pinelliae Pedatisectae and Rhizoma Typhonii Flagelliformis. Addressing this challenge, a direct quadruplex real-time VPCR approach has been developed in this study. Firstly, a direct lysis protocol has been implemented to facilitate the rapid release of genomic DNA within only 2 min. Secondly, specific primers and probes were designed for Pinellia ternata (Thunb.) Breit., Pinellia pedatisecta Schott and Typhonium flagelliforme (Lodd.) Blume, respectively. A conserved sequence on the ITS was also included as an internal control to mitigate the potential false negatives arising from DNA extraction or DNA amplification errors. Furthermore, the amplification process has been refined through a VPCR heating model, curtailing the detection to a scant 30 min. After a series of optimizations, the developed direct quadruplex VPCR allows for the simultaneous detection of these three plant materials, with each material having a detection sensitivity as low as 1 pg/µL. At last, the method's applicability was validated by using several commercial samples previously identified by Sanger sequencing.
半夏因其对健康的益处而闻名,经常被用于许多植物膳食补充剂和草药产品中。然而,由于需求量巨大,经常会掺入形态相似的物种,如Rhizoma Pinelliae Pedatisectae和Rhizoma Typhonii Flagelliformis。为了应对这一挑战,本研究开发了一种直接四重实时 VPCR 方法。首先,采用了直接裂解协议,以便在 2 分钟内快速释放基因组 DNA。其次,分别为 Pinellia ternata (Thunb.) Breit.、Pinellia pedatisecta Schott 和 Typhonium flagelliforme (Lodd.) Blume 设计了特异引物和探针。此外,还加入了 ITS 上的一个保守序列作为内部对照,以减少因 DNA 提取或 DNA 扩增错误而可能造成的假阴性。此外,扩增过程还通过 VPCR 加热模型进行了改进,将检测时间缩短至 30 分钟。经过一系列优化,所开发的直接四重 VPCR 可同时检测这三种植物材料,每种材料的检测灵敏度低至 1 pg/µL。最后,该方法的适用性还通过使用先前经桑格测序鉴定的几种商业样本进行了验证。
{"title":"Direct quadruplex real-time VPCR for simultaneous rapid detection of Pinelliae Rhizoma, Rhizoma Pinelliae Pedatisectae and Rhizoma Typhonii Flagelliformis","authors":"","doi":"10.1016/j.jfca.2024.106761","DOIUrl":"10.1016/j.jfca.2024.106761","url":null,"abstract":"<div><div>Known for its health benefits, <em>Pinelliae Rhizoma</em> is frequently utilized in many botanical dietary supplements and herbal products. However, the substantial demand has led to the frequent adulteration with morphologically similar species, such as <em>Rhizoma Pinelliae Pedatisectae</em> and <em>Rhizoma Typhonii Flagelliformis</em>. Addressing this challenge, a direct quadruplex real-time VPCR approach has been developed in this study. Firstly, a direct lysis protocol has been implemented to facilitate the rapid release of genomic DNA within only 2 min. Secondly, specific primers and probes were designed for <em>Pinellia ternata</em> (Thunb.) Breit., <em>Pinellia pedatisecta</em> Schott and <em>Typhonium flagelliforme</em> (Lodd.) Blume, respectively. A conserved sequence on the ITS was also included as an internal control to mitigate the potential false negatives arising from DNA extraction or DNA amplification errors. Furthermore, the amplification process has been refined through a VPCR heating model, curtailing the detection to a scant 30 min. After a series of optimizations, the developed direct quadruplex VPCR allows for the simultaneous detection of these three plant materials, with each material having a detection sensitivity as low as 1 pg/µL. At last, the method's applicability was validated by using several commercial samples previously identified by Sanger sequencing.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of roasting time on the color, protein, water distribution and key volatile compounds of pork 烘烤时间对猪肉色泽、蛋白质、水分分布和主要挥发性化合物的影响
IF 4 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-09-23 DOI: 10.1016/j.jfca.2024.106787
The study aimed to determine the changes in physical and chemical indicators in pork during the roasting process and establish predictive models for industrial intelligent production in the future, the effects of roasting times (0–15 min) on the dynamic changes in the surface color, water, protein and the volatile compounds in pork were investigated. The results showed that during the roasting process, the L* value increased firstly (0–3 min) and then decreased (3–15 min), the a* value decreased firstly (0–12 min) and then increased (12–15 min), while the b* value increased (0–15 min). A total of 29 volatile compounds were identified by gas chromatography–mass spectrometry, 11 volatile differentiators were identified by partial least squares-discriminant analysis, and 14 key volatile compounds were selected by odor activity values analysis. The total water content significantly decreased and the water in meat migrated from high degrees of freedom to low degrees of freedom. Roasting for 6 min was a key turning point in the changes of the α-helix, β-sheet and random coil. In addition, SDS-PAGE analysis, and the changes in the contents of carbonyl, total and free sulfhydryl verified proteins oxidation in the pork during the roasting process.
该研究旨在确定猪肉在焙烧过程中理化指标的变化,并建立未来工业智能化生产的预测模型,研究了焙烧时间(0-15 min)对猪肉表面色泽、水分、蛋白质和挥发性化合物动态变化的影响。结果表明,在焙烧过程中,L*值先上升(0-3 分钟)后下降(3-15 分钟),a*值先下降(0-12 分钟)后上升(12-15 分钟),而 b* 值上升(0-15 分钟)。气相色谱-质谱法共鉴定出 29 种挥发性化合物,偏最小二乘判别分析鉴定出 11 种挥发性分化物,气味活性值分析筛选出 14 种关键挥发性化合物。总含水量明显降低,肉中的水分从高自由度向低自由度迁移。焙烧 6 分钟是α-螺旋、β-片和无规线圈变化的关键转折点。此外,SDS-PAGE 分析以及羰基、总巯基和游离巯基含量的变化也证实了猪肉中的蛋白质在烘烤过程中发生了氧化。
{"title":"Impact of roasting time on the color, protein, water distribution and key volatile compounds of pork","authors":"","doi":"10.1016/j.jfca.2024.106787","DOIUrl":"10.1016/j.jfca.2024.106787","url":null,"abstract":"<div><div>The study aimed to determine the changes in physical and chemical indicators in pork during the roasting process and establish predictive models for industrial intelligent production in the future, the effects of roasting times (0–15 min) on the dynamic changes in the surface color, water, protein and the volatile compounds in pork were investigated. The results showed that during the roasting process, the <em>L</em>* value increased firstly (0–3 min) and then decreased (3–15 min), the <em>a</em>* value decreased firstly (0–12 min) and then increased (12–15 min), while the <em>b</em>* value increased (0–15 min). A total of 29 volatile compounds were identified by gas chromatography–mass spectrometry, 11 volatile differentiators were identified by partial least squares-discriminant analysis, and 14 key volatile compounds were selected by odor activity values analysis. The total water content significantly decreased and the water in meat migrated from high degrees of freedom to low degrees of freedom. Roasting for 6 min was a key turning point in the changes of the <em>α</em>-helix, β-sheet and random coil. In addition, SDS-PAGE analysis, and the changes in the contents of carbonyl, total and free sulfhydryl verified proteins oxidation in the pork during the roasting process.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Food Composition and Analysis
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1