Hafize Uzun, Yagmur Ozge Turac Kosem, Mehmet Velidedeoglu, Pınar Kocael, Seyma Dumur, Osman Simsek
Background: Mammography, used for breast cancer (BC) screening, has limitations such as decreased sensitivity in dense breasts. Currently used tumor markers are insufficient in diagnosing breast cancer. In this study, we aimed to investigate the relationship between serum levels of synaptophysin-like protein 1 (SYPL1) and BC, as well as compare SYPL1 with other blood tumor markers. Method: The study group consisted of 80 female patients with a histopathological diagnosis of invasive BC and didn’t receive any radiotherapy/chemotherapy. The control group 72 women with noprevious history of breast disease and evaluated as Breast Imaging Reporting and Data Systems (BI-RADS 1-2) on imaging. Serum SYPL1, cancer antigen 15-3 (CA 15-3) and carcinoembryonic antigen (CEA) were measured in both groups. Results: The diagnostic values of SYPL1, CEA and CA15-3 proteins in diagnosing BC were statistically significant. The sensitivity of SYPL1 was 48.75%, with a specificity of 80.56%. CA15-3 had a sensitivity of 80% and a specificity of 49.30%. There wasn’t statistically significant correlation between serum SYPL1 and tumor diameter, lymph node metastasis, distant organ metastasis, and stage.
Conclusion: The serum SYPL1 maintained a higher discriminatory ability for BC. The serum SYPL1 level can be used with high specificity in the diagnosis of BC. Although SYPL1 has low diagnostic value in BC by itself.
背景:乳房x线摄影，用于乳腺癌筛查，有局限性，如在致密乳房敏感性降低。目前使用的肿瘤标志物对乳腺癌的诊断不足。在本研究中，我们旨在探讨血清突触素样蛋白1 (SYPL1)水平与BC的关系，并将SYPL1与其他血液肿瘤标志物进行比较。方法:研究组80例经组织病理学诊断为浸润性BC的女性患者，未接受任何放疗/化疗。对照组为72名既往无乳腺疾病病史的女性，采用乳腺影像学报告和数据系统(BI-RADS 1-2)进行影像学评估。检测两组患者血清SYPL1、癌抗原15-3 (CA 15-3)、癌胚抗原(CEA)水平。结果:SYPL1、CEA、CA15-3蛋白对BC的诊断价值有统计学意义。SYPL1的敏感性为48.75%，特异性为80.56%。CA15-3的敏感性为80%，特异性为49.30%。血清SYPL1与肿瘤直径、淋巴结转移、远处脏器转移、分期无统计学意义。& # x0D;结论:血清SYPL1对BC具有较高的鉴别能力。血清SYPL1水平可作为诊断BC的高特异性指标。虽然SYPL1本身对BC的诊断价值较低。
{"title":"The Clinical significance of serum synaptophysin like 1 protein levels in breast cancer","authors":"Hafize Uzun, Yagmur Ozge Turac Kosem, Mehmet Velidedeoglu, Pınar Kocael, Seyma Dumur, Osman Simsek","doi":"10.5937/jomb0-46198","DOIUrl":"https://doi.org/10.5937/jomb0-46198","url":null,"abstract":"Background: Mammography, used for breast cancer (BC) screening, has limitations such as decreased sensitivity in dense breasts. Currently used tumor markers are insufficient in diagnosing breast cancer. In this study, we aimed to investigate the relationship between serum levels of synaptophysin-like protein 1 (SYPL1) and BC, as well as compare SYPL1 with other blood tumor markers. Method: The study group consisted of 80 female patients with a histopathological diagnosis of invasive BC and didn’t receive any radiotherapy/chemotherapy. The control group 72 women with noprevious history of breast disease and evaluated as Breast Imaging Reporting and Data Systems (BI-RADS 1-2) on imaging. Serum SYPL1, cancer antigen 15-3 (CA 15-3) and carcinoembryonic antigen (CEA) were measured in both groups. Results: The diagnostic values of SYPL1, CEA and CA15-3 proteins in diagnosing BC were statistically significant. The sensitivity of SYPL1 was 48.75%, with a specificity of 80.56%. CA15-3 had a sensitivity of 80% and a specificity of 49.30%. There wasn’t statistically significant correlation between serum SYPL1 and tumor diameter, lymph node metastasis, distant organ metastasis, and stage. 
 Conclusion: The serum SYPL1 maintained a higher discriminatory ability for BC. The serum SYPL1 level can be used with high specificity in the diagnosis of BC. Although SYPL1 has low diagnostic value in BC by itself.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To study the roles of micro ribonucleic acid (miR)-155 host gene (MIR155HG) and tumor necrosis factor-α (TNF-α) in the evaluation of prognosis of patients with systemic lupus erythematosus (SLE).
Methods: A total of 130 patients with SLE admitted to our hospital were selected, and the SLE disease activity index (SLEDAI) score was given. The expressions of MIR155HG and TNF-α were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), the incidence of complications during treatment was observed, and the associations of MIR155HG and TNF-α with SLEDAI before treatment and complications were analyzed. All patients were followed up after discharge, and the related factors to the prognosis of patients were analyzed via Cox regression analysis.
Results: The levels of MIR155HG and TNF-α were higher in patients with an SLEDAI score of 10-14 points than those in patients with an SLEDAI score of 5-9 points and 0-4 points. MIR155HG and TNF-α were positively correlated with the incidence of infection, renal damage and cardiac damage. Moreover, there was also a positive correlation between the expressions of serum MIR155HG and TNF-α in SLE patients. SLEDAI score ≥10 points, complications during hospitalization, and highly-expressed MIR155HG and TNF-α were risk factors related to the prognosis of patients.
Conclusion: MIR155HG and TNF-α are key regulators in the pathogenesis of SLE, and they can affect the prognosis of patients. Such a finding provides potential new targets for the treatment of SLE.
{"title":"Roles of MIR155HG and TNF-α in Evaluation of Prognosis of Patients with Systemic Lupus Erythematosus","authors":"Xiaojing Gu, Hu Chen, Rongping Li, Dibin Guo","doi":"10.5937/jomb0-45870","DOIUrl":"https://doi.org/10.5937/jomb0-45870","url":null,"abstract":"Background: To study the roles of micro ribonucleic acid (miR)-155 host gene (MIR155HG) and tumor necrosis factor-α (TNF-α) in the evaluation of prognosis of patients with systemic lupus erythematosus (SLE).
 Methods: A total of 130 patients with SLE admitted to our hospital were selected, and the SLE disease activity index (SLEDAI) score was given. The expressions of MIR155HG and TNF-α were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), the incidence of complications during treatment was observed, and the associations of MIR155HG and TNF-α with SLEDAI before treatment and complications were analyzed. All patients were followed up after discharge, and the related factors to the prognosis of patients were analyzed via Cox regression analysis.
 Results: The levels of MIR155HG and TNF-α were higher in patients with an SLEDAI score of 10-14 points than those in patients with an SLEDAI score of 5-9 points and 0-4 points. MIR155HG and TNF-α were positively correlated with the incidence of infection, renal damage and cardiac damage. Moreover, there was also a positive correlation between the expressions of serum MIR155HG and TNF-α in SLE patients. SLEDAI score ≥10 points, complications during hospitalization, and highly-expressed MIR155HG and TNF-α were risk factors related to the prognosis of patients.
 Conclusion: MIR155HG and TNF-α are key regulators in the pathogenesis of SLE, and they can affect the prognosis of patients. Such a finding provides potential new targets for the treatment of SLE.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135462194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Multiple sclerosis is one of the most common demyelinating diseases of the central nervous system. We aimed to investigate serum and cerebrospinal fluid levels of different laboratory inflammatory biomarkers in patients with MS.
Methods: A total of 120 subjects participated in the study, 60 of whom are diagnosed with MS, 30 with the final diagnose of non-inflammatory diseases of the CNS, and 30 healthy subjects representing the control group. Regarding to progression of radiological findings after 2 years from the initial diagnosis, MS group was divided into subgroups: with stationary radiological findings (n=30) and with radiologically proven disease progression (n=30). In all patients we analyzed levels of laboratory inflammatory biomarkers: CRP, NLR, GDF15 and NFs. Values of NFs and GDF15 were analyzed initial while the values of CRP and NLR were analyzed initial and after two years.
Results: We found statistically lower GDF15 values and initial CRP values in MS group in regards to group with non-inflammatory diseases of the CNS (p=0.000). On the other side, we determined significant elevation of laboratory markers CRP and NLR, initial and after two years period, in MS subgroup with progression of MRI findings (p= 0.000 and p=0.050, respectively). Also we found a positive correlation of CRP and neurofilaments (r=0.243, p=0.04), as well as a positive correlation of CRP and GDF15 in patients with MS (r=0.769, p=0.000).
Conclusion: We found significant elevation of laboratory markers of systemic inflammation, CRP and NLR in MS patients who developed disease progression based on MRI findings.
Key words: Multiple sclerosis; Neutrophil to lymphocyte ratio; C reactive protein, Neurofilaments, Growth differentiation factor 15
{"title":"Determination of systemic inflamatory biomarkers in multiple sclerosis","authors":"Maša Todorović, Stanislava Nikolić, Željko Živanović, Svetlana Simić, Lorand Sakalaš, Igor Spasić, Branislava Ilinčić, Velibor Čabarkapa","doi":"10.5937/jomb0-45083","DOIUrl":"https://doi.org/10.5937/jomb0-45083","url":null,"abstract":"Background: Multiple sclerosis is one of the most common demyelinating diseases of the central nervous system. We aimed to investigate serum and cerebrospinal fluid levels of different laboratory inflammatory biomarkers in patients with MS.
 Methods: A total of 120 subjects participated in the study, 60 of whom are diagnosed with MS, 30 with the final diagnose of non-inflammatory diseases of the CNS, and 30 healthy subjects representing the control group. Regarding to progression of radiological findings after 2 years from the initial diagnosis, MS group was divided into subgroups: with stationary radiological findings (n=30) and with radiologically proven disease progression (n=30). In all patients we analyzed levels of laboratory inflammatory biomarkers: CRP, NLR, GDF15 and NFs. Values of NFs and GDF15 were analyzed initial while the values of CRP and NLR were analyzed initial and after two years.
 Results: We found statistically lower GDF15 values and initial CRP values in MS group in regards to group with non-inflammatory diseases of the CNS (p=0.000). On the other side, we determined significant elevation of laboratory markers CRP and NLR, initial and after two years period, in MS subgroup with progression of MRI findings (p= 0.000 and p=0.050, respectively). Also we found a positive correlation of CRP and neurofilaments (r=0.243, p=0.04), as well as a positive correlation of CRP and GDF15 in patients with MS (r=0.769, p=0.000).
 Conclusion: We found significant elevation of laboratory markers of systemic inflammation, CRP and NLR in MS patients who developed disease progression based on MRI findings.
 Key words: Multiple sclerosis; Neutrophil to lymphocyte ratio; C reactive protein, Neurofilaments, Growth differentiation factor 15","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135615987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To investigate the expression levels and diagnostic value of glial cell line-derived neurotrophic factor (GDNF), carcinoembryonic antigen (CEA) and carbohydrate antigen199 (CA199) in patients with colorectal carcinoma (CRC).
Methods. 50 CRC patients at our hospital from Feb. 2020 to Feb. 2021 were chosen as the malignant group, another 50 patients with benign colonic diseases were chosen as the benign group, and 50 healthy people who came to our hospital for physical examination during the same period were considered as the control group. Fasting peripheral venous blood was taken from all research subjects in the morning and tested by a fully-automated electrochemiluminometer to determine the GDNF, CEA and CA199 levels. The sensitivity and specificity of the combined detection of the three indexes for CRC were analyzed, and the ROC curve was plotted to record the area under the curve (AUC).
Results. The malignant group had remarkably higher CEA and CA199 levels (P<0.001) and a lower GDNF level (P<0.001) when compared with the benign and control groups. The sensitivity, specificity, positive predictive value and negative predictive value of the combined detection were 96.0%, 94.0%, 88.9% and 97.9%, respectively. The combined detection had the AUC (95% CI) = 0.950 (0.909-0.991), the standard error of 0.021, and the progressive Sig.b<0.001.
Conclusion. The combined diagnosis of serum GDNF, CEA and CA199 is a reliable method to improve the diagnostic accuracy of CRC, and this strategy can effectively reduce the missed diagnosis rate and has high application value in clinic.
{"title":"Expression levels and diagnostic value of serum GDNF, CEA and CA199 in patients with Colorectal Carcinoma","authors":"Jue Wang, Lulu Liu, Yan Zheng, Sai Gu","doi":"10.5937/jomb0-44745","DOIUrl":"https://doi.org/10.5937/jomb0-44745","url":null,"abstract":"Background: To investigate the expression levels and diagnostic value of glial cell line-derived neurotrophic factor (GDNF), carcinoembryonic antigen (CEA) and carbohydrate antigen199 (CA199) in patients with colorectal carcinoma (CRC). 
 Methods. 50 CRC patients at our hospital from Feb. 2020 to Feb. 2021 were chosen as the malignant group, another 50 patients with benign colonic diseases were chosen as the benign group, and 50 healthy people who came to our hospital for physical examination during the same period were considered as the control group. Fasting peripheral venous blood was taken from all research subjects in the morning and tested by a fully-automated electrochemiluminometer to determine the GDNF, CEA and CA199 levels. The sensitivity and specificity of the combined detection of the three indexes for CRC were analyzed, and the ROC curve was plotted to record the area under the curve (AUC). 
 Results. The malignant group had remarkably higher CEA and CA199 levels (P<0.001) and a lower GDNF level (P<0.001) when compared with the benign and control groups. The sensitivity, specificity, positive predictive value and negative predictive value of the combined detection were 96.0%, 94.0%, 88.9% and 97.9%, respectively. The combined detection had the AUC (95% CI) = 0.950 (0.909-0.991), the standard error of 0.021, and the progressive Sig.b<0.001. 
 Conclusion. The combined diagnosis of serum GDNF, CEA and CA199 is a reliable method to improve the diagnostic accuracy of CRC, and this strategy can effectively reduce the missed diagnosis rate and has high application value in clinic.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Geng, Jinli Shi, Weina Guo, Haiyan Li, Dan Yang, Yan Gao
Background: It is an important clinical means to identify benign and malignant breast diseases caused by nipple discharge through the detection and analysis of components in nipple discharge. This study was aimed to test the expression and clinical significance of carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153) and carcinoembryonic antigen (CEA) in nipple discharge of breast cancer patients.
Methods: From January 2017 to December 2018, 86 patients with invasive ductal carcinoma of the breast with nipple discharge (breast cancer group) and 50 patients with ordinary breast duct hyperplasia with nipple discharge (benign control group) were selected, and the nipple discharge and serum CA125, CA153 and CEA levels in the two groups were detected by electrochemiluminescence immunoassay.
Results: The nipple discharge and serum CA125, CA153 and CEA levels in the benign control group were significantly lower than those in the breast cancer group, and the serum CA125, CA153 and CEA levels were obviously lower than those nipple discharge levels. The expression levels of CA125, CA153 and CEA in nipple discharge in the breast cancer group had no significant difference in different age of onset and different tumor sites. The CA125, CA153 and CEA levels in nipple discharge of patients with tumor diameter n5 cm, low differentiation, high stage, metastasis, and recurrence were obviously elevated versus to those of patients with tumor diameter <5 cm, high differentiation, low stage, and no metastasis and recurrence. These levels were not significantly correlated with the expression of estrogen receptor (ER) and progesterone receptor (PR), but was significantly correlated with the expression of human epidermal growth factor receptor (HER-2) and Ki-67. The accuracy, sensitivity, and negative predictive value of nipple discharge CA125, CA153 and CEA combined tests in the diagnosis of breast cancer were markedly improved compared with serum combinations and individual tests.
Conclusion: The combination of CA125, CA153 and CEA in nipple discharge can be considered as a potential diagnostic method for breast cancer, which is an effective supplement to serological diagnosis, and can provide new ideas for the differential diagnosis of benign and malignant breast cancer with nipple discharge.
{"title":"Expression and clinical significance of CA125, CA153 and CEA in nipple discharge of breast cancer patients","authors":"Jun Geng, Jinli Shi, Weina Guo, Haiyan Li, Dan Yang, Yan Gao","doi":"10.5937/jomb0-45192","DOIUrl":"https://doi.org/10.5937/jomb0-45192","url":null,"abstract":"Background: It is an important clinical means to identify benign and malignant breast diseases caused by nipple discharge through the detection and analysis of components in nipple discharge. This study was aimed to test the expression and clinical significance of carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153) and carcinoembryonic antigen (CEA) in nipple discharge of breast cancer patients. 
 Methods: From January 2017 to December 2018, 86 patients with invasive ductal carcinoma of the breast with nipple discharge (breast cancer group) and 50 patients with ordinary breast duct hyperplasia with nipple discharge (benign control group) were selected, and the nipple discharge and serum CA125, CA153 and CEA levels in the two groups were detected by electrochemiluminescence immunoassay.
 Results: The nipple discharge and serum CA125, CA153 and CEA levels in the benign control group were significantly lower than those in the breast cancer group, and the serum CA125, CA153 and CEA levels were obviously lower than those nipple discharge levels. The expression levels of CA125, CA153 and CEA in nipple discharge in the breast cancer group had no significant difference in different age of onset and different tumor sites. The CA125, CA153 and CEA levels in nipple discharge of patients with tumor diameter n5 cm, low differentiation, high stage, metastasis, and recurrence were obviously elevated versus to those of patients with tumor diameter <5 cm, high differentiation, low stage, and no metastasis and recurrence. These levels were not significantly correlated with the expression of estrogen receptor (ER) and progesterone receptor (PR), but was significantly correlated with the expression of human epidermal growth factor receptor (HER-2) and Ki-67. The accuracy, sensitivity, and negative predictive value of nipple discharge CA125, CA153 and CEA combined tests in the diagnosis of breast cancer were markedly improved compared with serum combinations and individual tests.
 Conclusion: The combination of CA125, CA153 and CEA in nipple discharge can be considered as a potential diagnostic method for breast cancer, which is an effective supplement to serological diagnosis, and can provide new ideas for the differential diagnosis of benign and malignant breast cancer with nipple discharge.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136308928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: To assess the influence of serum level of YAP on laboratory examination findings, imaging findings and disease activity of rheumatoid arthritis patients combined cardiovascular disease (RA-CVD).
Methods: RA-CVD patients (n=60), RA-nCVD patients (n=60) and healthy subjects (n=60) were recruited. Serum levels of YAP in them were detected by qRT-PCR. Their baseline characteristics were analyzed and compared. Disease activity, CVD risk factors and imaging findings in RA-CVD and RA-nCVD patients were evaluated and compared. In addition, potential influences of YAP on disease activity, CVD risk factors and imaging findings in RA-CVD patients were assessed.
Results: RA-CVD patients had higher levels of ERS, anti-CCP, RF, HDL-C, CRP, FRS, BNP, LA, LVs, LVd and cIMT, and lower level of EF in comparison to RA-nCVD patients. Serum level of YAP was higher in RA-CVD patients than that of RA-nCVD patients and healthy subjects. YAP level was positively correlated to DAS28, TG, CRP, PLT, FRS, BNP and cIMT in RA-CVD patients.
Conclusion: Serum level of YAP increases in RA-CVD patients. YAP is a potential factor driving the development of CVD in RA patients through regulating inflammatory response, lipid metabolism, glycometabolism and thrombosis.
{"title":"YAP drives the development of cardiovascular disease in patients with rheumatoid arthritis","authors":"Guozhu Che, Ying Liu, Na Zhang, Jing Zhao","doi":"10.5937/jomb0-45932","DOIUrl":"https://doi.org/10.5937/jomb0-45932","url":null,"abstract":"Background: To assess the influence of serum level of YAP on laboratory examination findings, imaging findings and disease activity of rheumatoid arthritis patients combined cardiovascular disease (RA-CVD). 
 Methods: RA-CVD patients (n=60), RA-nCVD patients (n=60) and healthy subjects (n=60) were recruited. Serum levels of YAP in them were detected by qRT-PCR. Their baseline characteristics were analyzed and compared. Disease activity, CVD risk factors and imaging findings in RA-CVD and RA-nCVD patients were evaluated and compared. In addition, potential influences of YAP on disease activity, CVD risk factors and imaging findings in RA-CVD patients were assessed. 
 Results: RA-CVD patients had higher levels of ERS, anti-CCP, RF, HDL-C, CRP, FRS, BNP, LA, LVs, LVd and cIMT, and lower level of EF in comparison to RA-nCVD patients. Serum level of YAP was higher in RA-CVD patients than that of RA-nCVD patients and healthy subjects. YAP level was positively correlated to DAS28, TG, CRP, PLT, FRS, BNP and cIMT in RA-CVD patients. 
 Conclusion: Serum level of YAP increases in RA-CVD patients. YAP is a potential factor driving the development of CVD in RA patients through regulating inflammatory response, lipid metabolism, glycometabolism and thrombosis.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136309098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: to observe the basic metabolic characteristics of obese patients with PCOS, and observe and compare the effect of laparoscopic sleeve gastrectomy and metformin treatment after 3 months.
Methods: In January to December 2018, the Second Hospital of Hebei Medical University selected 104 women who were classified as obese with a BMI of 28kg/cm2 or higher and had PCOS. They were divided into obese PCOS group (53 cases) and obese non-pcos group (51 cases).
Results: 1. There was no significant difference in waist circumference and WHR between patients who are obese with PCOS and patients who are obese without PCOS (P > 0.05).Obese PCOS patients were significantly higher in AMH, LH/FSH, T, FAI, homa-ir, TG, LDL, apo-b and uric acid than the group of non-PCOS patients who were obese. (P<0.05). The SHBG levels of obese patients with PCOS were obviously lower when contrasted with the levels in obese patients without PCOS (P < 0.05). 2. Body weight, BMI, INS, homa-ir and TG of obese PCOS patients were significantly decreased 3 months after laparoscopic sleeve gastrectomy compared with that before surgery (P < 0.05). After three months of medical treatment with metformin, the patients' HOMA-IR was obviously reduced when contrasted with the pre-treatment HOMA-IR levels (P < 0.05), and there was no significant difference in the improvement degree of homa-ir between the two groups (P > 0.05).
Conclusion: 1. Obese patients with PCOS demonstrated higher expression of AMH, LH/FSH, T, SHBG, and FAI when contrasted with the control group. Additionally, they experienced more severe insulin resistance and lipid metabolism disorders. 2. The weight and BMI of obese PCOS patients were significantly decreased after weight loss, while IR and blood lipid were significantly improved, while IR was improved in metformin group, and no significant discrepancy was observed in the degree of improvement of insulin resistance between both groups.
{"title":"Clinical observation of laparoscopic sleeve gastrectomy and metformin treatment in obese PCOS patients","authors":"Qingya Ma, Xiaojing He, Zijie Fu, Xiaodong Ren, Ranran Sun, Siqi Zhu, Xiaodong Li, Yahui Bian","doi":"10.5937/jomb0-44411","DOIUrl":"https://doi.org/10.5937/jomb0-44411","url":null,"abstract":"Background: to observe the basic metabolic characteristics of obese patients with PCOS, and observe and compare the effect of laparoscopic sleeve gastrectomy and metformin treatment after 3 months.
 Methods: In January to December 2018, the Second Hospital of Hebei Medical University selected 104 women who were classified as obese with a BMI of 28kg/cm2 or higher and had PCOS. They were divided into obese PCOS group (53 cases) and obese non-pcos group (51 cases).
 Results: 1. There was no significant difference in waist circumference and WHR between patients who are obese with PCOS and patients who are obese without PCOS (P > 0.05).Obese PCOS patients were significantly higher in AMH, LH/FSH, T, FAI, homa-ir, TG, LDL, apo-b and uric acid than the group of non-PCOS patients who were obese. (P<0.05). The SHBG levels of obese patients with PCOS were obviously lower when contrasted with the levels in obese patients without PCOS (P < 0.05). 2. Body weight, BMI, INS, homa-ir and TG of obese PCOS patients were significantly decreased 3 months after laparoscopic sleeve gastrectomy compared with that before surgery (P < 0.05). After three months of medical treatment with metformin, the patients' HOMA-IR was obviously reduced when contrasted with the pre-treatment HOMA-IR levels (P < 0.05), and there was no significant difference in the improvement degree of homa-ir between the two groups (P > 0.05).
 Conclusion: 1. Obese patients with PCOS demonstrated higher expression of AMH, LH/FSH, T, SHBG, and FAI when contrasted with the control group. Additionally, they experienced more severe insulin resistance and lipid metabolism disorders. 2. The weight and BMI of obese PCOS patients were significantly decreased after weight loss, while IR and blood lipid were significantly improved, while IR was improved in metformin group, and no significant discrepancy was observed in the degree of improvement of insulin resistance between both groups.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135785789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lidija Tulić, None Ivan, Jelena Stojnic, Jovan Bila, Zeljka Vukovic, Boba Kotlica
Background: This study aimed to examine if there is a connection between recombinant FSH dose and OS parameters in serum and the impact on IVF outcome. Methods: This study consisted of 101 participants that went through IVF procedures. Parameter that were tested are SOD, SH groups and MDA. Serum samples were drawn before stimulation and on the last day of ovarian stimulation. Results: Two groups were formed according to the dose of gonadotropins (rFSH). In both groups there were no significant differences in live–birth rate and miscarriage. In both groups mean serum MDA and SH-groups were significantly higher after ovarian stimulation, but mean serum SOD was significantly lower when compared to values before stimulation. There were less patients without OS before stimulation. Conclusion: Our results suggest that there is a difference in serum concentration in groups SOD, SH groups and MDA at the beginning and at the end ovarian stimulation. On the other hand, dose of rFSH is not related with change of parameters for oxidative stress, quality of oocytes, embryos, fertilization, pregnancies, and miscarriage rate. Patients without oxidative stress before the IVF procedure needed lower doses of gonadotropins during stimulation. Keywords: in vitro fertilization, oxidative stress, gonadotropin dose
{"title":"DIFFERENT DOSES OF RECOMBINANT FSH AND DETERMINING PARAMETERS OF OXIDATIVE STRESS","authors":"Lidija Tulić, None Ivan, Jelena Stojnic, Jovan Bila, Zeljka Vukovic, Boba Kotlica","doi":"10.5937/jomb0-46156","DOIUrl":"https://doi.org/10.5937/jomb0-46156","url":null,"abstract":"Background: This study aimed to examine if there is a connection between recombinant FSH dose and OS parameters in serum and the impact on IVF outcome. \u0000Methods: This study consisted of 101 participants that went through IVF procedures. Parameter that were tested are SOD, SH groups and MDA. Serum samples were drawn before stimulation and on the last day of ovarian stimulation. \u0000Results: Two groups were formed according to the dose of gonadotropins (rFSH). In both groups there were no significant differences in live–birth rate and miscarriage. In both groups mean serum MDA and SH-groups were significantly higher after ovarian stimulation, but mean serum SOD was significantly lower when compared to values before stimulation. There were less patients without OS before stimulation. \u0000Conclusion: Our results suggest that there is a difference in serum concentration in groups SOD, SH groups and MDA at the beginning and at the end ovarian stimulation. On the other hand, dose of rFSH is not related with change of parameters for oxidative stress, quality of oocytes, embryos, fertilization, pregnancies, and miscarriage rate. Patients without oxidative stress before the IVF procedure needed lower doses of gonadotropins during stimulation. \u0000Keywords: in vitro fertilization, oxidative stress, gonadotropin dose","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136023557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zihan Jin, Lu Xiao, Xinyi Xu, Changhong Miao, Yi Liu
Background: The relationship between triglyceride glucose (TyG) index and the incidence of acute kidney injury (AKI) in patients with acute myocardial infarction (AMI) is unclear. This study aims to explore the relationship between the two.
Methods: Participants were enrolled from Medical Information Mart for Intensive Care IV (MIMICIV) and grouping of subjects based on the quartile interval of the TyG index. With the presence of AKI as the main outcome, a logistic regression model was constructed. The correlation of the TyG index with the results obtained was examined by using a restricted cubic spline (RCS) model.
Results: Among the 1,101 AMI participants, 64.7% were male patients and the overall incidence rate of AKI was 37.1%. The results of multivariate Logistic regression analysis (LRA) revealed independent correlation of the TyG index with increased AKI risk (odds ratio (OR) = 2.23; confidence interval (CI) = 1.59 to 3.17; P < 0.001). Q4 (9.5-12.208) in the TyG index quartile was independently correlated with increased AKI risk (OR = 2.99, 95% CI = 1.64 to 5.46, P < 0.001). The RCS model showed that the AKI risk increased linearly as the TyG index increased (P=0.313).
Conclusion: In patients with AMI, the incidence of TyG and AKI is closely related. Nevertheless, further studies are needed to verify the finding.
{"title":"Association between triglyceride-glucose index and acute kidney injury in patients with acute myocardial infarction based on Medical Information Mart for Intensive Care database: A cross-sectional study","authors":"Zihan Jin, Lu Xiao, Xinyi Xu, Changhong Miao, Yi Liu","doi":"10.5937/jomb0-45219","DOIUrl":"https://doi.org/10.5937/jomb0-45219","url":null,"abstract":"Background: The relationship between triglyceride glucose (TyG) index and the incidence of acute kidney injury (AKI) in patients with acute myocardial infarction (AMI) is unclear. This study aims to explore the relationship between the two.
 Methods: Participants were enrolled from Medical Information Mart for Intensive Care IV (MIMICIV) and grouping of subjects based on the quartile interval of the TyG index. With the presence of AKI as the main outcome, a logistic regression model was constructed. The correlation of the TyG index with the results obtained was examined by using a restricted cubic spline (RCS) model.
 Results: Among the 1,101 AMI participants, 64.7% were male patients and the overall incidence rate of AKI was 37.1%. The results of multivariate Logistic regression analysis (LRA) revealed independent correlation of the TyG index with increased AKI risk (odds ratio (OR) = 2.23; confidence interval (CI) = 1.59 to 3.17; P < 0.001). Q4 (9.5-12.208) in the TyG index quartile was independently correlated with increased AKI risk (OR = 2.99, 95% CI = 1.64 to 5.46, P < 0.001). The RCS model showed that the AKI risk increased linearly as the TyG index increased (P=0.313).
 Conclusion: In patients with AMI, the incidence of TyG and AKI is closely related. Nevertheless, further studies are needed to verify the finding.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136144039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Murat Caglayan, Ataman Gonel, Cuneyt Tayman, Ufuk Cakir, Ismail Koyuncu, Ebru Temiz, Yasemin Sert
Background: Routine screening for hereditary disorders in newborns includes screening for treatable metabolic and endocrine disorders, such as biotidinase deficiency, galactosemia, maple syrup urine disease, hypothyroidism, and cystic fibrosis. Incorrect test results may be encountered due to the use of vitamin K1. To investigate the interference effect of vitamin K1 on neonatal screening tests and to raise awareness of erroneous measurements.
Methods: Heel blood samples were taken from 25 newborns born in a neonatal intensive care unit. Dry blood C0, C2, C3, C4, C4DC, C5:1, C5OH, C5DC, C6, C6DC, C8, C8:1, C8DC, C10, C10:1, C10DC, C12, C14, C14:1, C14:2, C16, C16:1, C18, C18:1, C18:2, C18:OH, methylglutaryl, valine, leucine/isoleucine, methionine, phenylalanine, argininosuccinic acid, aspartate, alanine, arginine, citrulline, glycine, ornithine, and glutamate tests were studied using the tandem mass spectrometry (MS) method. The results of the heel blood samples obtained before and after the application of vitamin K1 (Phyto menadione) were compared.
Results: In two studies conducted with in vitro and in vivo tests, C0, C2, C3, C4, C4DC, C5, C5OH, C6, C8, C10, C10:1, C14, C16, C16:1, C18, C18:1, methylglutaryl, phenylalanine, argininosuccinic acid, tyrosine, aspartate, arginine, citrulline, glycine, and glutamine were all significantly elevated (p < 0.05).
Conclusions: Heel blood samples may yield false results due to vitamin K1 administration. In the case of doubtful results, a new sample should be taken and the measurement should be repeated.
{"title":"Investigation of the effect of vitamin K1 prophylaxis on newborn screenings tests in newborns.","authors":"Murat Caglayan, Ataman Gonel, Cuneyt Tayman, Ufuk Cakir, Ismail Koyuncu, Ebru Temiz, Yasemin Sert","doi":"10.5937/jomb0-40162","DOIUrl":"10.5937/jomb0-40162","url":null,"abstract":"<p><strong>Background: </strong>Routine screening for hereditary disorders in newborns includes screening for treatable metabolic and endocrine disorders, such as biotidinase deficiency, galactosemia, maple syrup urine disease, hypothyroidism, and cystic fibrosis. Incorrect test results may be encountered due to the use of vitamin K1. To investigate the interference effect of vitamin K1 on neonatal screening tests and to raise awareness of erroneous measurements.</p><p><strong>Methods: </strong>Heel blood samples were taken from 25 newborns born in a neonatal intensive care unit. Dry blood C0, C2, C3, C4, C4DC, C5:1, C5OH, C5DC, C6, C6DC, C8, C8:1, C8DC, C10, C10:1, C10DC, C12, C14, C14:1, C14:2, C16, C16:1, C18, C18:1, C18:2, C18:OH, methylglutaryl, valine, leucine/isoleucine, methionine, phenylalanine, argininosuccinic acid, aspartate, alanine, arginine, citrulline, glycine, ornithine, and glutamate tests were studied using the tandem mass spectrometry (MS) method. The results of the heel blood samples obtained before and after the application of vitamin K1 (Phyto menadione) were compared.</p><p><strong>Results: </strong>In two studies conducted with in vitro and in vivo tests, C0, C2, C3, C4, C4DC, C5, C5OH, C6, C8, C10, C10:1, C14, C16, C16:1, C18, C18:1, methylglutaryl, phenylalanine, argininosuccinic acid, tyrosine, aspartate, arginine, citrulline, glycine, and glutamine were all significantly elevated (p < 0.05).</p><p><strong>Conclusions: </strong>Heel blood samples may yield false results due to vitamin K1 administration. In the case of doubtful results, a new sample should be taken and the measurement should be repeated.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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