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Dyslipidaemia and inflammatory markers as the risk predictors for cardiovascular disease in newly diagnosed premenopausal hypothyroid women. 血脂异常和炎症标志物作为新诊断的绝经前甲状腺功能减退妇女心血管疾病的危险预测因素
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-37007
Vaideki Balamurugan, Ravindra Maradi, Vivek Joshi, Belle Vijetha Shenoy, Manjunatha B K Goud

Background: Hypothyroidism can predispose systolic and diastolic cardiac dysfunction, increased peripheral vascular resistance, endothelial dysfunction, altered coagulopathy, and dyslipidemia resulting in atherosclerosis. Thyroid hormones can influence homocysteine metabolism by regulating the methylenetetrahydrofolate reductase (M THR). So, this study aimed to compare the markers homocysteine, high sensitive C-reactive protein (hs-CRP), and Atherogenic Indices (AI) between newly diagnosed hypothyroid and euthyroid premenopausal women.

Methods: 80 Female patients between 20 and 45 years were enrolled in this study and were equally divided into cases and controls group. Laboratory tests included: i) Serum T3, T4, TSH was measured using electrochemiluminescence, ii) lipid profile, homocysteine, and hs-CRP were measured for all the participants. Atherogenic indices: Castelli risk indices I&II, Atherogenic coefficient (AEC), and Atherogenic Index of Plasma (AIP) were calculated using formulas. A comparison between the study groups was made by using the Independent t-test and Mann-Whitney U test. p-value < 0.05 was considered significant.

Results: The hypothyroid group had significantly higher levels of homocysteine (p= 0.014), and hs-CRP (hs-CRP> 3.0 mg/L, 70% of participants have intermediate to high risk for a cardiovascular event) and elevated BMI compared to participants in the euthyroid group. Atherogenic indices (p< 0.001) was significantly increased in the hypothyroid participants' group. TC, TG , and LDL were significantly elevated in the hypothyroid group but did not show any association with systolic and diastolic blood pressure.

Conclusions: Premenopausal women with hypothyroidism have a greater predisposition for cardiovascular disease compared to euthyroid.

背景:甲状腺功能减退可诱发收缩期和舒张期心功能障碍、外周血管阻力增加、内皮功能障碍、凝血功能改变和血脂异常导致动脉粥样硬化。甲状腺激素可通过调节亚甲基四氢叶酸还原酶(mthr)影响同型半胱氨酸代谢。因此,本研究旨在比较新诊断为甲状腺功能减退和甲状腺功能正常的绝经前妇女的同型半胱氨酸、高敏c反应蛋白(hs-CRP)和动脉粥样硬化指数(AI)。方法:选取80例年龄在20 ~ 45岁的女性患者,将其平均分为病例组和对照组。实验室测试包括:i)使用电化学发光法测量血清T3、T4、TSH, ii)测量所有参与者的血脂、同型半胱氨酸和hs-CRP。致动脉粥样硬化指数:采用公式计算Castelli风险指数i和ii、致动脉粥样硬化系数(Atherogenic coefficient, AEC)和血浆致动脉粥样硬化指数(Atherogenic Index of Plasma, AIP)。各研究组间的比较采用独立t检验和Mann-Whitney U检验。p值< 0.05为显著性。结果:与甲状腺功能正常组相比,甲状腺功能减退组的同型半胱氨酸水平显著升高(p= 0.014), hs-CRP (hs-CRP> 3.0 mg/L, 70%的参与者有心血管事件的中高风险)和BMI升高。甲状腺功能减退组的动脉粥样硬化指数显著升高(p< 0.001)。甲状腺功能减退组TC、TG和LDL显著升高,但与收缩压和舒张压没有任何关联。结论:与甲状腺功能正常的妇女相比,绝经前甲状腺功能减退的妇女更容易患心血管疾病。
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引用次数: 2
Oxidative stress-related risk of the multiple sclerosis development. 氧化应激相关的多发性硬化症发展风险。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-37546
Marija Vasić, Aleksandra Topić, Bojan Marković, Neda Milinković, Evica Dinčić

Background: Multiple sclerosis (MS) is characterized by inflammation, demyelination and axonal degeneration. Oxidative stress (OS) plays a significant role in the pathogenesis of the disease. The aim of the study was to examine the association between OS and smoking on the MS development.

Methods: The study included 175 patients with relapsing-remitting multiple sclerosis (RRMS) (76 males, 99 females) and 254 healthy subjects (81 males and 173 females). Oxidative stress biomarkers in serum, Total Antioxidant Status (TAS) and Total Oxidative Status (TOS) were determined spectrophotometrically. Oxidative Stress Index (OSI) was calculated as the ratio of TOS and TAS. Urinary 8-oxo7,8-dihydro-2'-deoxyguanosine were determined by HPLC-MS/MS and expressed as 8-oxodG/creatinine.

Results: In females with RRMS were higher TOS, OSI and 8-oxodG/creatinine than in females in control group. The group of males with RRMS had lower level of TAS than the males in control group. Higher levels of 8-oxodG/creatinine was obtained in active, passive and former smokers with RRMS than in control group with the same exposition to tobacco smoke. Independent predictors of MS are passive smoking, increased OSI and increased levels of urinary 8-oxodG/creatinine.

Conclusions: Our results demonstrate that the OS parameters should be included in the assessment of the risk for MS development. Due to the more sensitivity to oxidative stress, females may be at higher risk of MS development. This data indicates the importance of introducing the antioxidant therapy as a complementary treatment in patients with RRMS.

背景:多发性硬化症(MS)以炎症、脱髓鞘和轴突变性为特征。氧化应激(OS)在该疾病的发病机制中起重要作用。这项研究的目的是检查多发性硬化症和吸烟对多发性硬化症发展的关系。方法:研究对象为175例复发-缓解型多发性硬化症(RRMS)患者(男性76例,女性99例)和254例健康受试者(男性81例,女性173例)。采用分光光度法测定血清氧化应激生物标志物、总抗氧化状态(TAS)和总氧化状态(TOS)。氧化应激指数(OSI)计算为TOS与TAS的比值。采用HPLC-MS/MS法测定尿8-氧- 7,8-二氢-2′-脱氧鸟苷,并以8-氧- g /肌酐表达。结果:RRMS患者TOS、OSI、8-oxodG/肌酐均高于对照组。RRMS男性组TAS水平低于对照组。主动吸烟者、被动吸烟者和RRMS前吸烟者的8-oxodG/肌酐水平均高于接触相同烟草烟雾的对照组。MS的独立预测因子是被动吸烟、OSI升高和尿8-氧合g /肌酐水平升高。结论:我们的研究结果表明,应将OS参数纳入MS发展风险的评估中。由于对氧化应激更敏感,女性发生多发性硬化症的风险更高。这一数据表明,在RRMS患者中引入抗氧化治疗作为补充治疗的重要性。
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引用次数: 2
Prognosis and clinical features analysis of EMT-related signature and tumor Immune microenvironment in glioma. 胶质瘤 EMT 相关特征和肿瘤免疫微环境的预后和临床特征分析
IF 2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-39234
Zheng Xiao, Xiaoyan Liu, Yixiang Mo, Weibo Chen, Shizhong Zhang, Yingwei Yu, Huiwen Weng

Background: As the most common primary malignant intracranial tumor, glioblastoma has a poor prognosis with limited treatment options. It has a high propensity for recurrence, invasion, and poor immune prognosis due to the complex tumor microenvironment.

Methods: Six groups of samples from four datasets were included in this study. We used consensus ClusterPlus to establish two subgroups by the EMT-related gene. The difference in clinicopathological features, genomic characteristics, immune infiltration, treatment response and prognoses were evaluated by multiple algorithms. By using LASSO regression, multi-factor Cox analysis, stepAIC method, a prognostic risk model was constructed based on the final screened genes.

Results: The consensusClusterPlus analyses revealed two subtypes of glioblastoma (C1 and C2), which were characterized by different EMT-related gene expression patterns. C2 subtype with the worse prognosis had the more malignant clinical and pathology manifestations, higher Immune infiltration and tumor-associated molecular pathways scores, and poorer response to treatment. Additionally, our EMT-related genes risk prediction model can provide valuable support for clinical evaluations of glioma.

Conclusions: The assessment system and prediction model displayed good performance in independent prognostic risk assessment and individual patient treatment response prediction. This can help with clinical treatment decisions and the development of effective treatments.

背景:作为最常见的原发性颅内恶性肿瘤,胶质母细胞瘤预后较差,治疗方案有限。由于肿瘤微环境复杂,它具有高复发倾向、高侵袭性和免疫预后差等特点:本研究纳入了来自四个数据集的六组样本。我们使用共识聚类软件(ClusterPlus)根据 EMT 相关基因建立了两个亚组。通过多种算法评估了临床病理特征、基因组特征、免疫浸润、治疗反应和预后的差异。利用 LASSO 回归、多因素 Cox 分析和 stepAIC 方法,根据最终筛选出的基因构建了预后风险模型:结果:ClusterPlus 共识分析显示胶质母细胞瘤有两种亚型(C1 和 C2),它们的 EMT 相关基因表达模式各不相同。预后较差的C2亚型具有更恶性的临床和病理表现、更高的免疫浸润和肿瘤相关分子通路评分以及更差的治疗反应。此外,我们的EMT相关基因风险预测模型可以为胶质瘤的临床评估提供有价值的支持:该评估系统和预测模型在独立预后风险评估和个体患者治疗反应预测方面表现良好。结论:该评估系统和预测模型在独立预后风险评估和个体患者治疗反应预测方面表现良好,有助于临床治疗决策和有效治疗方法的开发。
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引用次数: 0
Association of octacosanol supplementation with redox status in patients on chronic statin therapy. 八烷醇补充与慢性他汀类药物治疗患者氧化还原状态的关系。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-38224
Milica Zrnić-Ćirić, Jelena Kotur-Stevuljević, Ivan Stanković, Brižita Đordjević, Ivana Baralić, Miodrag Ostojić

Background: The uneven lipid-lowering statin effects and statin intolerance raise interest regarding the involvement of coadministration of statins and dietary supplements. This study aimed to evaluate the effects of octacosanol supplementation on markers of redox status in cardiovascular patients on chronic atorvastatin therapy.

Methods: A double-blind, randomized, placebo-controlled, single-centre study was conducted. Redox status homeostasis parameters [i.e., advanced oxidation protein products (AOPP), pro-oxidant-antioxidant balance (PAB), total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase activity (SOD), total protein sulfhydryl (SHgroups), and paraoxonase 1 (PO N 1) activity] were assessed in 81 patients. According to favorable changes in lipid profile, patients were classified into two groups: responders (n = 35) and non-responders (n = 46), and followed for 13 weeks. A principal component analysis (PCA) was applied to explore the effect of octacosanol supplementation and the relationship between investigated parameters as predictors of responders' and non-responders' status.

Results: Significant decrease in Oxy-score value was found at the endpoint compared to baseline in responders' group (21.0 (13.4-25.5) versus 15.1 (12.4-18.0); P < 0.01). PCA analysis extracted 4 significant factors in the both groups, whereas extracted factors containing "octacosanol status" variable explained 14.7% and 11.5% of the variance in responders' and non-responders' subgroups, respectively.

Conclusions: Octacosanol supplementation leads to an improvement of lipid profile and markers of redox status in responders' group. New studies are needed to validate our results in order to find the best approach for personalized supplementation as a useful adjunct to standard statin therapy.

背景:他汀类药物降脂效果不均匀和他汀类药物不耐受引起了人们对他汀类药物和膳食补充剂联合使用的兴趣。本研究旨在评估补充八烷醇对慢性阿托伐他汀治疗心血管患者氧化还原状态标志物的影响。方法:采用双盲、随机、安慰剂对照、单中心研究。评估了81例患者的氧化还原状态稳态参数[即晚期氧化蛋白产物(AOPP)、促氧化-抗氧化平衡(PAB)、总氧化状态(TOS)、总抗氧化状态(TAS)、超氧化物歧化酶活性(SOD)、总蛋白巯基(SHgroups)和对氧氧化酶1 (pon1)活性]。根据脂质谱的有利变化,将患者分为两组:有反应者(n = 35)和无反应者(n = 46),随访13周。应用主成分分析(PCA)探讨八烷沙醇补充的影响以及所调查参数作为反应者和无反应者状态的预测因子之间的关系。结果:与基线相比,应答者组在终点的oxy评分值显著下降(21.0 (13.4-25.5)vs 15.1 (12.4-18.0);P < 0.01)。PCA分析在两组中均提取了4个显著因子,其中包含“八烷醇状态”变量的提取因子在反应组和无反应组中分别解释了14.7%和11.5%的方差。结论:补充八烷糖醇可以改善应激反应组的血脂和氧化还原状态。需要新的研究来验证我们的结果,以便找到个性化补充的最佳方法,作为标准他汀类药物治疗的有用辅助。
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引用次数: 0
Associations of serum high-sensitivity C-reactive protein and prealbumin with coronary vessels stenosis determined by coronary angiography and heart failure in patients with myocardial infarction. 血清高敏c反应蛋白和前白蛋白与心肌梗死患者冠状动脉造影确定的冠状血管狭窄和心力衰竭的关系
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-37847
Yun Zhu, Zhen Yu, Ronggui Xu, Beibei Wang, Yiqun Lou, Na Zhang, Ziyin Chen

Background: To explore the associations of serum high-sensitivity C-reactive protein (hs-CRP) and prealbumin (PAB) with the number of diseased coronary vessels, degree of stenosis and heart failure in patients with myocardial infarction (MI).

Methods: A total of 39 MI patients treated in the Cardiology were selected as the observation group, and another 41 patients with normal results of coronary angiography during the same period were selected as the control group. The general data of patients were recorded in detail, the content of serum hs-CRP and PAB in the peripheral blood was detected, and the number of diseased coronary vessels and the degree of stenosis were detected via coronary angiography.

Results: Compared with those in control group, the blood pressure and heart rate significantly rose, the content of indexes related to the severity of MI were significantly increased, the content of hs-CRP was significantly increased, and the content of PAB was significantly decreased in observation group. Hs-CRP was positively correlated with the number of diseased coronary vessels, degree of stenosis and heart failure in patients, but PAB was negatively correlated with the above factors. The survival rate of MI patients with high content of hs-CRP was obviously lower than that of patients with low content of hsCRP.

Conclusions: Serum hs-CRP and PAB are closely associated with the number of diseased coronary vessels, degree of stenosis and heart failure in MI patients.

背景:探讨血清高敏c反应蛋白(hs-CRP)和前白蛋白(PAB)与心肌梗死(MI)患者冠状动脉病变数、狭窄程度和心力衰竭的关系。方法:选取心内科治疗的心肌梗死患者39例作为观察组,同期冠状动脉造影结果正常的患者41例作为对照组。详细记录患者一般资料,检测外周血hs-CRP、PAB含量,并通过冠状动脉造影检测病变冠状血管数及狭窄程度。结果:与对照组比较,观察组患者血压、心率均明显升高,心肌梗死严重程度相关指标含量均显著升高,hs-CRP含量显著升高,PAB含量显著降低。Hs-CRP与患者冠脉病变数、狭窄程度、心力衰竭呈正相关,PAB与上述因素呈负相关。hs-CRP含量高的心肌梗死患者生存率明显低于hs-CRP含量低的心肌梗死患者。结论:心肌梗死患者血清hs-CRP、PAB与冠脉病变数、狭窄程度及心力衰竭密切相关。
{"title":"Associations of serum high-sensitivity C-reactive protein and prealbumin with coronary vessels stenosis determined by coronary angiography and heart failure in patients with myocardial infarction.","authors":"Yun Zhu,&nbsp;Zhen Yu,&nbsp;Ronggui Xu,&nbsp;Beibei Wang,&nbsp;Yiqun Lou,&nbsp;Na Zhang,&nbsp;Ziyin Chen","doi":"10.5937/jomb0-37847","DOIUrl":"https://doi.org/10.5937/jomb0-37847","url":null,"abstract":"<p><strong>Background: </strong>To explore the associations of serum high-sensitivity C-reactive protein (hs-CRP) and prealbumin (PAB) with the number of diseased coronary vessels, degree of stenosis and heart failure in patients with myocardial infarction (MI).</p><p><strong>Methods: </strong>A total of 39 MI patients treated in the Cardiology were selected as the observation group, and another 41 patients with normal results of coronary angiography during the same period were selected as the control group. The general data of patients were recorded in detail, the content of serum hs-CRP and PAB in the peripheral blood was detected, and the number of diseased coronary vessels and the degree of stenosis were detected via coronary angiography.</p><p><strong>Results: </strong>Compared with those in control group, the blood pressure and heart rate significantly rose, the content of indexes related to the severity of MI were significantly increased, the content of hs-CRP was significantly increased, and the content of PAB was significantly decreased in observation group. Hs-CRP was positively correlated with the number of diseased coronary vessels, degree of stenosis and heart failure in patients, but PAB was negatively correlated with the above factors. The survival rate of MI patients with high content of hs-CRP was obviously lower than that of patients with low content of hsCRP.</p><p><strong>Conclusions: </strong>Serum hs-CRP and PAB are closely associated with the number of diseased coronary vessels, degree of stenosis and heart failure in MI patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 1","pages":"9-15"},"PeriodicalIF":2.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9921086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10826238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnostic performance of lactate dehydrogenase (LDH) isoenzymes levels for the severity of COVID-19. 乳酸脱氢酶(LDH)同工酶水平对COVID-19严重程度的诊断价值
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-37234
Ilkay Ergenc, Emre Capar, Sengel Buket Erturk, Gunel Bahramzade, Fatih Atalah, Derya Kocakaya, Sait Karakurt, Goncagul Haklar, Zekaver Odabasi

Background: Lactate dehydrogenase (LDH) levels predict coronavirus disease 2019 (COVID-19) severity. We investigated LDH isoenzyme levels to identify the tissue responsible for serum LDH elevation in patients with COVID-19.

Methods: Hospitalised COVID-19 patients with serum LDH levels exceeding the upper reference limit included. LDH isoenzymes were detected quantitatively on agarose gels. The radiological severity of lung involvement on computed tomography was scored as 0-5 for each lobe (total possible score, 0-25). Disease severity was determined using the World Health Organization (WHO) clinical progression scale.

Results: In total, 111 patients (mean age, 59.96 ± 16.14), including 43 females (38.7%), were enrolled. The serum levels of total LDH and all five LDH isoenzymes were significantly higher in the severe group. The levels of all LDH isoenzymes excluding LDH5 positively correlated with the WHO score. LDH3 levels correlated with chest computed tomography findings (r2 = 0.267, p = 0.005). On multivariate analysis, LDH3 was an independent risk factor for the deterioration of COVID-19.

Conclusions: LDH3 appears to be an independent risk factor for deterioration in patients with COVID-19. LDH elevation in patients with COVID-19 predominantly resulted from lung, liver and muscle damage.

背景:乳酸脱氢酶(LDH)水平可预测2019冠状病毒病(COVID-19)的严重程度。我们研究了LDH同工酶水平,以确定导致COVID-19患者血清LDH升高的组织。方法:纳入血清LDH水平超过参考上限的住院COVID-19患者。琼脂糖凝胶定量检测LDH同工酶。计算机断层扫描肺受累的放射学严重程度评分为0-5分(可能的总评分为0-25分)。采用世界卫生组织(WHO)临床进展量表确定疾病严重程度。结果:共纳入111例患者,平均年龄59.96±16.14岁,其中女性43例(38.7%)。重度组血清总LDH及5种LDH同工酶水平均显著升高。除LDH5外的所有LDH同工酶水平与WHO评分呈正相关。LDH3水平与胸部计算机断层扫描结果相关(r2 = 0.267, p = 0.005)。多因素分析显示,LDH3是COVID-19恶化的独立危险因素。结论:LDH3可能是COVID-19患者病情恶化的独立危险因素。COVID-19患者的LDH升高主要由肺、肝和肌肉损伤引起。
{"title":"Diagnostic performance of lactate dehydrogenase (LDH) isoenzymes levels for the severity of COVID-19.","authors":"Ilkay Ergenc,&nbsp;Emre Capar,&nbsp;Sengel Buket Erturk,&nbsp;Gunel Bahramzade,&nbsp;Fatih Atalah,&nbsp;Derya Kocakaya,&nbsp;Sait Karakurt,&nbsp;Goncagul Haklar,&nbsp;Zekaver Odabasi","doi":"10.5937/jomb0-37234","DOIUrl":"https://doi.org/10.5937/jomb0-37234","url":null,"abstract":"<p><strong>Background: </strong>Lactate dehydrogenase (LDH) levels predict coronavirus disease 2019 (COVID-19) severity. We investigated LDH isoenzyme levels to identify the tissue responsible for serum LDH elevation in patients with COVID-19.</p><p><strong>Methods: </strong>Hospitalised COVID-19 patients with serum LDH levels exceeding the upper reference limit included. LDH isoenzymes were detected quantitatively on agarose gels. The radiological severity of lung involvement on computed tomography was scored as 0-5 for each lobe (total possible score, 0-25). Disease severity was determined using the World Health Organization (WHO) clinical progression scale.</p><p><strong>Results: </strong>In total, 111 patients (mean age, 59.96 ± 16.14), including 43 females (38.7%), were enrolled. The serum levels of total LDH and all five LDH isoenzymes were significantly higher in the severe group. The levels of all LDH isoenzymes excluding LDH5 positively correlated with the WHO score. LDH3 levels correlated with chest computed tomography findings (r<sup>2</sup> = 0.267, <i>p</i> = 0.005). On multivariate analysis, LDH3 was an independent risk factor for the deterioration of COVID-19.</p><p><strong>Conclusions: </strong>LDH3 appears to be an independent risk factor for deterioration in patients with COVID-19. LDH elevation in patients with COVID-19 predominantly resulted from lung, liver and muscle damage.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 1","pages":"16-26"},"PeriodicalIF":2.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10767995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The assessment value of pathological condition of serum adiponectin and amylin in primary osteoporosis and its correlation analysis with bone metabolism indexes. 血清脂联素、胰淀素病理状态在原发性骨质疏松症中的评价价值及其与骨代谢指标的相关性分析。
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-35877
Xiao Jie Wang, Xue Bai, Ying Miu, Pan Chen, Pi Jun Yan, Chun Xia Jiang

Background: This paper explores the assessment value of pathological condition of serum adiponectin (APN) and amylin in primary osteoporosis (POP) and their correlation with bone metabolism indexes.

Methods: From January 2019 to June 2021, 79 cases of POP patients were selected as the research objects. A test of the patients' bone density was conducted, and clinical grading of POP was via T value (normal, mild, moderate, severe). The analysis of the assessment value of pathological condition of serum APN and amylin for POP and their association with bone metabolism indexes in patients was performed.

Results: APN and amylin in patients were declined with POP's aggravation. APN of 5.15 μg/mL or less and amylin of 15.38 pmol/L or less were risk factors influencing the aggravation of pathological condition of POP (P< 0 .0 5). The area under the curve (AUC) of combined detection of APN and amylin to assess the severity of POP was elevated vs. alone test of amylin (P< 0.05). 25-hydroxyvitamin D (25-(OH) D) and total type 1 procollagen amino-terminal propeptide (t-PINP) in patients were descended with the aggravation of pathological condition of osteoporosis (P < 0.05). At the same time, no distinct differences were presented in the three groups of type I collagen hydroxyl terminal peptide b degradation product (β-CTX) and N-terminal osteocalcin (N-MID) (P> 0.05). APN, amylin, 25-(OH)D, β-CTX, and t-PINP were negatively linked with POP clinical grade (P< 0.05). APN and amylin were associated with 25-(OH) D, β-CTX, t-PINP (P< 0.05), and APN and amylin were not linked with N-MID (P> 0.05).

Conclusions: Serum APN and amylin are provided with evaluation values for the severity of POP and are associated with bone metabolism in patients.

背景:探讨血清脂联素(APN)和胰淀素(amylin)病理状态在原发性骨质疏松症(POP)中的评价价值及其与骨代谢指标的相关性。方法:选取2019年1月~ 2021年6月79例POP患者作为研究对象。对患者进行骨密度检测,通过T值对POP进行临床分级(正常、轻度、中度、重度)。分析患者血清APN和胰淀素病理状态对POP的评估价值及其与骨代谢指标的相关性。结果:随着POP的加重,APN和胰淀素呈下降趋势。APN为5.15 μg/mL及以下、amylin为15.38 pmol/L及以下是影响POP病理状况加重的危险因素(P< 0.05),联合检测APN和amylin评估POP严重程度的曲线下面积(AUC)较单独检测amylin升高(P< 0.05)。25-羟基维生素D (25-(OH) D)和总1型前胶原氨基末端前肽(t-PINP)随骨质疏松病理状况的加重而下降(P < 0.05)。同时,三组ⅰ型胶原羟基末端肽b降解产物(β-CTX)和n端骨钙素(N-MID)均无显著差异(P> 0.05)。APN、胰淀素、25-(OH)D、β-CTX、t-PINP与POP临床分级呈负相关(P< 0.05)。APN和amylin与25-(OH) D、β-CTX、t-PINP相关(P< 0.05),而APN和amylin与N-MID无关(P> 0.05)。结论:血清APN和胰淀素对POP的严重程度具有评价价值,并与患者骨代谢有关。
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引用次数: 0
Interactions of the eNOS and ACE genes and cigarette smoking in chronic obstructive pulmonary disease. eNOS和ACE基因与慢性阻塞性肺疾病吸烟的相互作用
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-34017
Marija Stanković, Valentina Đorđević, Andrija Tomović, Ljudmila Nagorni-Obradović, Nataša Petrović-Stanojević, Mirjana Kovač, Dragica Radojković

Background: Chronic obstructive pulmonary disease (COPD) is a complex disorder with unexplained heritability. Interactions of genetic and environmental factors are thought to be crucial in COPD. So, we aim to examine interactions of the endothelial nitric oxide synthase (eNOS) and angiotensin converting enzyme (ACE) genes and cigarette smoking in COPD.

Methods: The eNOS G 894T and ACE ID variants were analyzed in 122 COPD patients and 200 controls from Serbia. The effect of the variants on COPD was assessed by logistic regression. Interactions between eNOS, ACE and cigarette smoking in COPD were evaluated using a case-control model. Interaction between the genes was analyzed in silico.

Results: No effect of the eNOS G 894T and ACE ID variants on COPD was found in our study. Gene-gene interaction between the eN OS T T and A CE D was identified (p=0.033) in COPD. The interaction is realized within the complex network of biochemical pathways. Gene-environment interactions between the eNOS T and cigarette smoking (p=0.013), and the ACE II and cigarette smoking (p=0.009) were detected in COPD in our study.

Conclusions: This is the first research to reveal interactions of the eNOS and ACE genes and cigarette smoking in COPD progressing our understanding of COPD heritability and contributing to the development of appropriate treatments.

背景:慢性阻塞性肺疾病(COPD)是一种遗传性不明的复杂疾病。遗传和环境因素的相互作用被认为是COPD的关键。因此,我们的目的是研究内皮型一氧化氮合酶(eNOS)和血管紧张素转换酶(ACE)基因与COPD患者吸烟的相互作用。方法:对来自塞尔维亚的122例COPD患者和200例对照进行eNOS G 894T和ACE ID变异分析。通过logistic回归评估这些变异对COPD的影响。采用病例-对照模型评估eNOS、ACE与COPD患者吸烟之间的相互作用。在计算机上分析了基因之间的相互作用。结果:在我们的研究中未发现eNOS G 894T和ACE ID变异对COPD的影响。在COPD患者中发现enos T T和A CE D之间的基因-基因相互作用(p=0.033)。这种相互作用是在生化途径的复杂网络中实现的。在COPD患者中检测到eNOS T与吸烟之间的基因-环境相互作用(p=0.013), ACE II与吸烟之间的基因-环境相互作用(p=0.009)。结论:这是第一个揭示eNOS和ACE基因与吸烟在COPD中的相互作用的研究,促进了我们对COPD遗传性的理解,并有助于开发适当的治疗方法。
{"title":"Interactions of the eNOS and ACE genes and cigarette smoking in chronic obstructive pulmonary disease.","authors":"Marija Stanković,&nbsp;Valentina Đorđević,&nbsp;Andrija Tomović,&nbsp;Ljudmila Nagorni-Obradović,&nbsp;Nataša Petrović-Stanojević,&nbsp;Mirjana Kovač,&nbsp;Dragica Radojković","doi":"10.5937/jomb0-34017","DOIUrl":"https://doi.org/10.5937/jomb0-34017","url":null,"abstract":"<p><strong>Background: </strong>Chronic obstructive pulmonary disease (COPD) is a complex disorder with unexplained heritability. Interactions of genetic and environmental factors are thought to be crucial in COPD. So, we aim to examine interactions of the endothelial nitric oxide synthase (eNOS) and angiotensin converting enzyme (ACE) genes and cigarette smoking in COPD.</p><p><strong>Methods: </strong>The eNOS G 894T and ACE ID variants were analyzed in 122 COPD patients and 200 controls from Serbia. The effect of the variants on COPD was assessed by logistic regression. Interactions between eNOS, ACE and cigarette smoking in COPD were evaluated using a case-control model. Interaction between the genes was analyzed in silico.</p><p><strong>Results: </strong>No effect of the eNOS G 894T and ACE ID variants on COPD was found in our study. Gene-gene interaction between the eN OS T T and A CE D was identified (p=0.033) in COPD. The interaction is realized within the complex network of biochemical pathways. Gene-environment interactions between the eNOS T and cigarette smoking (p=0.013), and the ACE II and cigarette smoking (p=0.009) were detected in COPD in our study.</p><p><strong>Conclusions: </strong>This is the first research to reveal interactions of the eNOS and ACE genes and cigarette smoking in COPD progressing our understanding of COPD heritability and contributing to the development of appropriate treatments.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 1","pages":"94-104"},"PeriodicalIF":2.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10767999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pregnancy outcomes and newborn characteristics in women with follicular fluid thyroid autoantibodies undergoing assisted reproduction. 卵泡液甲状腺自身抗体妇女辅助生殖的妊娠结局和新生儿特征
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-35243
Sanja Medenica, Eliana Garalejić, Džihan Abazović, Zoran Bukumirić, Stavroula A Paschou, Biljana Arsić, Snežana Vujošević, Biljana Međo, Miloš Žarković

Background: Higher levels of thyroid autoantibodies in follicular fluid (FF) of thyroid autoimmunity (TAI) positive women are strongly correlated with serum levels and may have effect on the post-implantation embryo development. Literature highlights that levothyroxine (LT4) treatment may attenuate the risk of adverse pregnancy outcomes. The aim of the study was to estimate the pregnancy and newborn outcomes in women with FF thyroid autoantibodies undergoing assisted reproductive technology (ART).

Methods: The study population included 24 women with confirmed clinical pregnancy, 8 TAI positive and 16 TAI negative women. LT4 supplementation was applied in 20.8% patients, TAI positive.

Results: Pregnancy outcomes were: twin pregnancy rate 41.7%, early miscarriage rate 8.3%, late miscarriage rate 4.2%, preterm birth rate 16.7%, term birth rate 70.8%, live birth rate 96.0%. There was significant difference in serum and in FF TgAbs (p< 0.001)between the groups according to TAI, while serum fT3 was lower in the group with TAI (p = 0.047). Serum P4 was higher in LT4 treated group (p = 0.005), with TAI, and newborns in this group had higher birth weight (p = 0.001) and height (p = 0.008). Maternal complications occurred in 23.8% of patients. No congenital malformations in newborns were noted.

Conclusions: Thyroid autoantibodies present in FF may have an effect on the post-implantation embryo development, but have no effect on further course of pregnancy. The special benefit of LT4 treatment for successful ART outcome was demonstrated for newborn anthropometric parameters.

背景:甲状腺自身免疫(TAI)阳性妇女卵泡液(FF)中甲状腺自身抗体水平升高与血清水平密切相关,可能对着床后胚胎发育有影响。文献强调,左旋甲状腺素(LT4)治疗可降低不良妊娠结局的风险。该研究的目的是评估患有FF甲状腺自身抗体的妇女接受辅助生殖技术(ART)的妊娠和新生儿结局。方法:纳入临床证实妊娠的24例,TAI阳性8例,TAI阴性16例。20.8%的患者补充LT4, TAI阳性。结果:妊娠结局:双胎妊娠率41.7%,早期流产率8.3%,晚期流产率4.2%,早产率16.7%,足月产率70.8%,活产率96.0%。TAI组间血清和FF TgAbs差异有统计学意义(p< 0.001),而TAI组血清fT3较低(p = 0.047)。LT4治疗组血清P4高于TAI组(p = 0.005),且LT4治疗组新生儿出生体重(p = 0.001)和身高(p = 0.008)均高于TAI组。产妇并发症发生率为23.8%。新生儿无先天性畸形。结论:FF中存在的甲状腺自身抗体可能对着床后胚胎发育有影响,但对妊娠的进一步发展没有影响。对于新生儿的人体测量参数,LT4治疗对成功的ART结果的特殊益处被证明。
{"title":"Pregnancy outcomes and newborn characteristics in women with follicular fluid thyroid autoantibodies undergoing assisted reproduction.","authors":"Sanja Medenica,&nbsp;Eliana Garalejić,&nbsp;Džihan Abazović,&nbsp;Zoran Bukumirić,&nbsp;Stavroula A Paschou,&nbsp;Biljana Arsić,&nbsp;Snežana Vujošević,&nbsp;Biljana Međo,&nbsp;Miloš Žarković","doi":"10.5937/jomb0-35243","DOIUrl":"https://doi.org/10.5937/jomb0-35243","url":null,"abstract":"<p><strong>Background: </strong>Higher levels of thyroid autoantibodies in follicular fluid (FF) of thyroid autoimmunity (TAI) positive women are strongly correlated with serum levels and may have effect on the post-implantation embryo development. Literature highlights that levothyroxine (LT4) treatment may attenuate the risk of adverse pregnancy outcomes. The aim of the study was to estimate the pregnancy and newborn outcomes in women with FF thyroid autoantibodies undergoing assisted reproductive technology (ART).</p><p><strong>Methods: </strong>The study population included 24 women with confirmed clinical pregnancy, 8 TAI positive and 16 TAI negative women. LT4 supplementation was applied in 20.8% patients, TAI positive.</p><p><strong>Results: </strong>Pregnancy outcomes were: twin pregnancy rate 41.7%, early miscarriage rate 8.3%, late miscarriage rate 4.2%, preterm birth rate 16.7%, term birth rate 70.8%, live birth rate 96.0%. There was significant difference in serum and in FF TgAbs (p< 0.001)between the groups according to TAI, while serum fT<sub>3</sub> was lower in the group with TAI (p = 0.047). Serum P<sub>4</sub> was higher in LT4 treated group (p = 0.005), with TAI, and newborns in this group had higher birth weight (p = 0.001) and height (p = 0.008). Maternal complications occurred in 23.8% of patients. No congenital malformations in newborns were noted.</p><p><strong>Conclusions: </strong>Thyroid autoantibodies present in FF may have an effect on the post-implantation embryo development, but have no effect on further course of pregnancy. The special benefit of LT4 treatment for successful ART outcome was demonstrated for newborn anthropometric parameters.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 1","pages":"27-33"},"PeriodicalIF":2.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9316465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Impact of the seventh day nucleated red blood cell count on mortality in COVID-19 intensive care unit patients: A retrospective case-control study. 重症监护病房患者第7天有核红细胞计数对死亡率的影响:一项回顾性病例对照研究
IF 2.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-01-20 DOI: 10.5937/jomb0-39839
Muhammed Emin Düz, Mustafa Arslan, Elif E Menek, Burak Yasin Avci

Background: COVID-19 covers a broad clinical spectrum, threatening global health. Although several studies have investigated various prognostic biochemical and hematological parameters, they generally lack specificity and are insufficient for decision-making. Beyond the neonatal period, NRBCs (nucleated red blood cells) in peripheral blood is rare and often associated with malignant neoplasms, bone marrow diseases, and other severe disorders such as sepsis and hypoxia. Therefore, we investigated if NRBCs can predict mortality in hypoxic ICU (Intensive Care Unit) patients of COVID-19.

Methods: Seventy-one unvaccinated RT-PCR confirmed COVID-19 ICU patients was divided into those who survived (n=35, mean age=58) and died (n=36, mean age=75). Venous blood samples were collected in K3 EDTA tubes and analyzed on a Sysmex XN-1000 hematology analyzer with semiconductor laser flow cytometry and nucleic acid fluorescence staining method for NRBC analysis. NRBC numbers and percentages of the patients were compared on the first and seventh days of admission to the ICU. Results are reported as a proportion of NRBCs per 100 WBCs NRBCs/100 WBC (NRBC% and as absolute NRBC count (NRBC #, × 109/L).

Results: NRBC 7th-day count and % values were statistically higher in non-survival ones. The sensitivity for 7th day NRBC value <0.01 (negative) was 86.11%, the specificity was 48.57%, for <0.02; 75.00%, and 77.14%, for <0.03; 61.11%, and 94.60%.

Conclusions: In conclusion, our results indicate that NRBC elevation (>0.01) significantly predicts mortality in ICU hospitalized patients due to COVID-19. Worse, a high mortality rate is expected, especially with NRBC values of >0.03.

背景:COVID-19涉及广泛的临床范围,威胁着全球健康。虽然有几项研究调查了各种预后生化和血液学参数,但它们通常缺乏特异性,不足以用于决策。在新生儿期之后,外周血中的nrbc(有核红细胞)是罕见的,通常与恶性肿瘤、骨髓疾病和其他严重疾病如败血症和缺氧有关。因此,我们研究nrbc是否可以预测COVID-19低氧ICU(重症监护病房)患者的死亡率。方法:将71例未接种RT-PCR疫苗的COVID-19 ICU确诊患者分为存活组(n=35,平均年龄58岁)和死亡组(n=36,平均年龄75岁)。静脉血经K3 EDTA管采集,在Sysmex XN-1000血液分析仪上采用半导体激光流式细胞仪和核酸荧光染色法进行NRBC分析。比较患者在入住ICU的第1天和第7天NRBC的数量和百分比。结果报告为每100个WBC中NRBC /100 WBC的比例(NRBC%)和绝对NRBC计数(NRBC #, × 109/L)。结果:非生存组NRBC第7天计数和%值均高于生存组。结论:NRBC升高(>0.01)对新冠肺炎ICU住院患者的死亡率有显著预测作用。更糟糕的是,预计死亡率高,特别是NRBC值>0.03。
{"title":"Impact of the seventh day nucleated red blood cell count on mortality in COVID-19 intensive care unit patients: A retrospective case-control study.","authors":"Muhammed Emin Düz,&nbsp;Mustafa Arslan,&nbsp;Elif E Menek,&nbsp;Burak Yasin Avci","doi":"10.5937/jomb0-39839","DOIUrl":"https://doi.org/10.5937/jomb0-39839","url":null,"abstract":"<p><strong>Background: </strong>COVID-19 covers a broad clinical spectrum, threatening global health. Although several studies have investigated various prognostic biochemical and hematological parameters, they generally lack specificity and are insufficient for decision-making. Beyond the neonatal period, NRBCs (nucleated red blood cells) in peripheral blood is rare and often associated with malignant neoplasms, bone marrow diseases, and other severe disorders such as sepsis and hypoxia. Therefore, we investigated if NRBCs can predict mortality in hypoxic ICU (Intensive Care Unit) patients of COVID-19.</p><p><strong>Methods: </strong>Seventy-one unvaccinated RT-PCR confirmed COVID-19 ICU patients was divided into those who survived (n=35, mean age=58) and died (n=36, mean age=75). Venous blood samples were collected in K3 EDTA tubes and analyzed on a Sysmex XN-1000 hematology analyzer with semiconductor laser flow cytometry and nucleic acid fluorescence staining method for NRBC analysis. NRBC numbers and percentages of the patients were compared on the first and seventh days of admission to the ICU. Results are reported as a proportion of NRBCs per 100 WBCs NRBCs/100 WBC (NRBC% and as absolute NRBC count (NRBC #, × 109/L).</p><p><strong>Results: </strong>NRBC 7th-day count and % values were statistically higher in non-survival ones. The sensitivity for 7th day NRBC value <0.01 (negative) was 86.11%, the specificity was 48.57%, for <0.02; 75.00%, and 77.14%, for <0.03; 61.11%, and 94.60%.</p><p><strong>Conclusions: </strong>In conclusion, our results indicate that NRBC elevation (>0.01) significantly predicts mortality in ICU hospitalized patients due to COVID-19. Worse, a high mortality rate is expected, especially with NRBC values of >0.03.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"42 1","pages":"138-144"},"PeriodicalIF":2.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9920868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10826237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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Journal of Medical Biochemistry
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