Journal of insect physiology最新文献_第2页

A potential role for the interaction of Wolbachia surface proteins with the Drosophila microtubulin in maintenance of endosymbiosis and affecting spermiogenesis 沃尔巴克氏体表面蛋白与果蝇微管蛋白相互作用在维持内共生和影响精子发生中的潜在作用。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.jinsphys.2024.104743

Bin Mao , Ying-Ying Wang , Si-Ying Li , Yue Fu , Yun-Li Xiao , Yu-Feng Wang

Wolbachia, as a widely infected intracellular symbiotic bacterium in Arthropoda, is able to manipulate the reproduction of insect hosts for facilitating their own transmission. Cytoplasmic incompatibility (CI) is the most common phenotype that Wolbachia induced in insect hosts where they resulted in the failure of uninfected egg hatch when fertilized with the sperm derived from Wolbachia-infected males, suggesting that the sperm are modified by Wolbachia during spermatogenesis. Although the molecular mechanisms of CI are beginning to be understood, the effects of Wolbachia on the symbiotic relationship and the proper dynamics of spermatogenesis have not yet been fully investigated. We report here that Wolbachia infection induced a significant upregulation of betaTub85D in the testis of Drosophila melanogaster. Knockdown of betaTub85D in fly testes resulted in significant decrease in the copy number of Wolbachia surface protein gene (wsp), indicating a notable reduction of Wolbachia density. Pull-down analyses revealed that WSP interacted with the betaTub85D of D. melanogaster. Wolbachia infection altered the interactome between betaTub85D and other proteins in the testes, and may thus change the protein synthesis and metabolic pathways. Wolbachia infection induced not only an interaction of betaTub85D with Mst77F but also increase in phosphorylated Mst77F. These results suggest that Wolbachia WSP protein might play important roles in anchoring the endosymbiont to the host’s cytoskeleton and consequently interfere the interactions among key proteins involved in spermatogenesis in the insect host testes, resulting in modified sperm.

沃尔巴克氏体是节肢动物中广泛感染的细胞内共生细菌，能够操纵昆虫宿主的繁殖以促进其自身的传播。细胞质不相容（Cytoplasmic incompatibility， CI）是沃尔巴克氏体在昆虫宿主中诱导的最常见的表型，当与沃尔巴克氏体感染的雄性精子受精时，它们导致未感染的卵子孵化失败，这表明精子在精子发生过程中被沃尔巴克氏体修饰。虽然CI的分子机制开始被理解，但沃尔巴克氏体对共生关系和精子发生的适当动力学的影响尚未得到充分的研究。我们在这里报道沃尔巴克氏体感染诱导黑腹果蝇睾丸中β ub85d的显著上调。敲低betaTub85D后，蝇睾丸沃尔巴克氏体表面蛋白基因（Wolbachia surface protein gene, wsp）表达显著降低，沃尔巴克氏体密度显著降低。下拉分析显示WSP与黑腹龙的betaTub85D相互作用。沃尔巴克氏体感染改变了睾丸中betaTub85D与其他蛋白质之间的相互作用，从而可能改变蛋白质的合成和代谢途径。沃尔巴克氏体感染不仅诱导betaTub85D与Mst77F相互作用，而且磷酸化的Mst77F也增加。这些结果表明，沃尔巴克氏体WSP蛋白可能在将内共生体锚定在宿主细胞骨架上，从而干扰昆虫睾丸中参与精子发生的关键蛋白之间的相互作用，从而导致精子被修饰。

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引用次数: 0

Adult brain neurogenesis does not account for behavioral differences between solitary and social bees 成年的脑神经发生不能解释独居蜜蜂和群居蜜蜂之间的行为差异。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.jinsphys.2024.104737

Iulia Antioch , Sarah Larnaudie , Isabelle Lafon, Jean-Marc Devaud, Claire Rampon , Raphaël Jeanson

In many taxa, increasing attention is being paid to how group living shapes the expression of brain plasticity and behavioural flexibility. In eusocial insects, the lifelong commitment of workers and queens to a reproductive or non-reproductive caste is accompanied by a loss of behavioural totipotency, and often, by the expression of a limited behavioural repertoire in workers due to their specialisation. On the other hand, individuals of solitary species have a broader behavioural repertoire as they have to perform all the tasks themselves. This raises the question of whether solitary and social insects differ in their levels of brain plasticity. One mechanism found in both invertebrates and vertebrates to contribute to brain plasticity is adult neurogenesis. It is a mechanism by which adult-born neurons are generated, differentiated and functionally integrated in the brain circuits during adulthood. In this study, we compared the solitary bee Osmia bicornis and the eusocial bee Apis mellifera. We focused on the mushroom bodies which are higher-order integration centres in the insect brain. Based on their known behavioural repertoire, our prediction was that both solitary and social bees would exhibit neurogenesis in the brain until the pupal stage, but that this capacity would persist only in adult solitary bees. However, our results do not validate this prediction, as they indicate that no cells are produced in the mushroom bodies or other areas of the adult solitary bee brain.

在许多类群中，人们越来越关注群居生活如何影响大脑可塑性和行为灵活性的表达。在群居昆虫中，工蚁和蚁后终生致力于生殖或非生殖种姓，伴随着行为全能性的丧失，工蚁往往由于其专业化而表现出有限的行为剧目。另一方面，独居物种的个体由于必须自己完成所有的任务，因此其行为剧目范围更广。这就提出了一个问题：独居昆虫和群居昆虫的大脑可塑性水平是否不同。在无脊椎动物和脊椎动物中发现的一种有助于大脑可塑性的机制是成体神经发生。它是一种机制，通过这种机制，成体出生的神经元在成年期生成、分化并在功能上整合到大脑回路中。在这项研究中，我们比较了独居蜂茭白和群居蜂Apis mellifera。我们重点研究了蘑菇体，它是昆虫大脑中的高阶整合中心。根据已知的行为剧目，我们预测独居蜂和群居蜂的大脑在蛹期之前都会出现神经发生，但只有成年独居蜂才会持续存在这种能力。然而，我们的研究结果并没有验证这一预测，因为研究结果表明，成年独居蜂大脑的蘑菇体或其他区域没有产生任何细胞。

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引用次数: 0

PKC phospho-activated PFK1 is required for PBAN regulated sex pheromone biosynthesis in Helicoverpa armigera Helicoverpa armigera 的性信息素生物合成需要 PKC 磷酸化激活的 PFK1。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.jinsphys.2024.104739

Ruolan He , Zelong She , Yao Zhang, Shuangyan Yao, Jizhen Wei, Mengfang Du, Shiheng An

The enzyme 6-phosphofructokinase-1 (PFK1) acts as the primary rate-limiting enzyme in glycolysis, catalyzing the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. This glycolytic process provides essential substrates for the synthesis of sex pheromones. However, the specific function of PFK1 in sex pheromone biosynthesis remains unidentified. This study aimed to investigate the detailed mechanism by which PFK1 influences pheromone biosynthesis activating neuropeptide (PBAN)-regulated sex pheromone biosynthesis in Hecoverpa armigera. Findings revealed the presence of two PFK genes in pheromone glands (PGs). Further investigation demonstrated that RNAi-mediated knockdown of PFK1 significantly reduced sex pheromone production, mating success and the female ability to attract males, whereas PFK2 did not influence sex pheromone biosynthesis. Importantly, PFK1 was activated by PBAN in both isolated PGs and Sf9 cells. However, PBAN-induced activation of PFK1 could be attenuated by chelerythrine chloride (CC), a specific inhibitor of protein kinase C (PKC). Furthermore, the phosphorylation levels of PFK1 significantly increased in response to PBAN challenge, while CC treatment significantly mitigated this phosphorylation. PFK1 activity was found to depend on phosphorylation at the S135 and S676 sites in response to PBAN stimulation. Mutants at these sites abolished PFK1 phosphorylation and its activity. Overall, our findings unveil a critical mechanism by which the PBAN signaling recruits PKC to phosphorylate PFK1 at S135 and S676 sites, thereby activating PFK1. This activation ensures the normal progression of the glycolysis pathway, ultimately facilitating sex pheromone biosynthesis.

6-磷酸果糖激酶-1（PFK1）是糖酵解过程中的主要限速酶，催化 6-磷酸果糖转化为 1,6-二磷酸果糖。这一糖酵解过程为性信息素的合成提供了必要的底物。然而，PFK1 在性信息素生物合成中的具体功能仍未确定。本研究旨在探究 PFK1 影响 Hecoverpa armigera 中由信息素生物合成激活神经肽（PBAN）调控的性信息素生物合成的详细机制。研究结果显示，信息素腺体（PGs）中存在两个 PFK 基因。进一步研究表明，RNAi介导的PFK1基因敲除会显著降低性信息素的产生、交配成功率和雌虫吸引雄虫的能力，而PFK2基因则不会影响性信息素的生物合成。重要的是，PFK1 在离体 PG 和 Sf9 细胞中均被 PBAN 激活。然而，氯化白屈菜红碱（CC）（一种特异性蛋白激酶 C（PKC）抑制剂）可以减轻 PBAN 诱导的 PFK1 激活。此外，PFK1 的磷酸化水平在 PBAN 挑战下明显升高，而 CC 处理可明显减轻这种磷酸化。研究发现，PFK1 的活性取决于 S135 和 S676 位点的磷酸化对 PBAN 刺激的反应。这些位点上的突变体可消除 PFK1 磷酸化及其活性。总之，我们的研究结果揭示了一个关键机制，PBAN 信号通过该机制招募 PKC 使 PFK1 在 S135 和 S676 位点磷酸化，从而激活 PFK1。这种激活确保了糖酵解途径的正常进行，最终促进了性信息素的生物合成。

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引用次数: 0

Cuticular nitrogen economy during development in the cockroach Cryptocercus punctulatus and the termite Neotermes jouteli 斑点隐尾虫和白蚁发育过程中表皮氮经济性的研究。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.jinsphys.2024.104745

D.E. Mullins , C.A. Nalepa , A.J. Mullins , S.E. Gabbert

The role of nitrogen during insect development and reproduction is key in the success of a species, and is of primary importance in wood feeding taxa. Based on comparison of xylophagous, one-piece termites to the termite sister group, subsocial wood-feeding cockroaches in the genus Cryptocercus, it has been proposed that the evolution of termite eusociality involved a fundamental shift in nitrogen allocation strategies. Cryptocercus exhibits a nitrogen storage economy, with individuals gradually increasing in size and cuticular density over a years-long developmental period. Termites, however, remain in a juvenilized morphotype with minimal investment into cuticle, suggesting that nitrogen is conserved and circulated according to the needs of the colony via behaviors such as trophallaxis and cannibalism. We examined the nitrogen economy of Cryptocercus punctulatus and the dampwood termite Neotermes jouteli, focusing on cuticular nitrogen investment during development and exuvial nitrogen losses resulting from molting. Cryptocercus progressively changes from a pale, thin, soft cuticle at hatch to a dark, thick, heavily sclerotized cuticle in adults; increases in N/mg cuticle and the quantity of cuticular catecholamines are correlated with these ontogenetic color changes. There were significant differences in the nitrogen content of two successive age classes of early stage juveniles and in their discarded exuvia at molt. Soldier and alate castes of N. jouteli exhibited the highest sclerotization/melanization indices; pseudergates had levels comparable to those measured in Class I (3rd and 4th instar) juveniles of C. punctulatus. Exuvia of N. jouteli contained 0.19 μgN/mg, while exuvia of approximately two- and three-year-old C. punctulatus had 72.9 and 82.6 μgN/mg, respectively. Our data support the hypothesis that the evolution of termite eusociality from subsocial cockroach ancestors was rooted in chronic fitness limitations imposed by their low nitrogen diet.

氮在昆虫发育和繁殖过程中的作用是物种成功的关键，对食木类群至关重要。通过对食木白蚁与隐尾虫属亚社会性食木蟑螂的比较，作者提出白蚁的社会性进化涉及氮分配策略的根本转变。隐尾蚴在长达数年的发育过程中，个体的大小和表皮密度逐渐增加，表现出氮储存经济性。然而，白蚁仍然处于幼体形态，角质层的投入很少，这表明氮是根据蚁群的需要通过营养和同类相食等行为保存和循环的。本文研究了点隐尾蚴（Cryptocercus punctulatus）和湿木白蚁（netermes jouteli）的氮经济，重点分析了发育过程中表皮氮的投入和蜕皮过程中脱落氮的损失。隐尾蚴从孵化时的苍白、薄、软的角质层逐渐转变为成虫时的深色、厚、严重硬化的角质层；N/mg角质层和角质层儿茶酚胺含量的增加与这些个体发生的颜色变化有关。不同龄期幼鱼的氮含量及蜕皮后的废皮中氮含量存在显著差异。士兵和贵族种姓的黑化/硬化指数最高；假门菌含量与I类（3龄和4龄）马尾松幼鱼相当。黄颡鱼和三岁左右的小针叶鱼的外皮含量分别为72.9 μgN/mg和82.6 μgN/mg。我们的数据支持了一种假设，即白蚁从亚社会性蟑螂祖先进化而来的群居性根植于它们的低氮饮食所施加的慢性适应性限制。

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引用次数: 0

Wolbachia infection modifies phloem feeding behavior but not plant virus transmission by a hemipteran host 沃尔巴克氏体感染改变韧皮部摄食行为，但不改变植物病毒通过半足类宿主传播。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2025-01-01 DOI: 10.1016/j.jinsphys.2024.104746

Gina M. Angelella, Jillian J. Foutz, Joanna Galindo-Schuller

Wolbachia-infected and uninfected subpopulations of beet leafhoppers, Circulifer tenellus (Baker) (Hemiptera: Cicadellidae), co-occur in the Columbia Basin region of Washington and Oregon. While facultative endosymbionts such as Hamiltonella defensa have demonstrably altered feeding/probing behavior in hemipteran hosts, the behavioral phenotypes conferred by Wolbachia to its insect hosts, including feeding/probing, are largely understudied. We studied the feeding/probing behavior of beet leafhoppers with and without Wolbachia using electropenetrography, along with corresponding inoculation rates of beet curly top virus, a phloem-limited plant pathogen vectored by beet leafhoppers. Insects carrying the virus with and without Wolbachia were individually recorded for four hours while interacting with a potato plant, and wavelengths annotated following established conventions. Virus inoculation rates and the duration of phloem salivation events did not vary. Wolbachia-infected insects more than tripled the duration of phloem ingestion, but despite this, Wolbachia infection was linked with marginally lower, not enhanced, acquisition. Regardless, results suggest potential for Wolbachia to increase the acquisition rate of other phloem-limited plant pathogens.

感染沃尔巴克氏体和未感染沃尔巴克氏体的甜菜叶蝉亚群，圆叶蝉（半翅目：圆叶蝉科），共同出现在华盛顿和俄勒冈州的哥伦比亚盆地地区。虽然兼性内共生菌如防御汉氏菌已经明显改变了半足动物宿主的进食/探测行为，但沃尔巴克氏菌赋予昆虫宿主的行为表型，包括进食/探测，在很大程度上还没有得到充分的研究。利用电渗透技术研究了带沃尔巴克氏体和不带沃尔巴克氏体的甜菜跳叶蝉的取食/探测行为，以及相应的甜菜卷顶病毒（一种由甜菜跳叶蝉传播的韧皮部限制植物病原体）的接种量。携带沃尔巴克氏体病毒和不携带沃尔巴克氏体病毒的昆虫分别与马铃薯植物相互作用4小时，并按照既定惯例对波长进行注释。病毒接种率和韧皮部唾液分泌事件的持续时间没有变化。感染沃尔巴克氏体的昆虫摄取韧皮部的时间增加了两倍多，但尽管如此，沃尔巴克氏体感染与略微降低（而不是增强）的获取有关。无论如何，结果表明沃尔巴克氏体有可能增加其他韧皮部限制的植物病原体的获得率。

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引用次数: 0

Autophagy in the retina affects photoreceptor synaptic plasticity and behavior 视网膜的自噬影响感光细胞突触的可塑性和行为。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2024-12-09 DOI: 10.1016/j.jinsphys.2024.104741

Aleksandra Tyszka , Kornel Szypulski , Elzbieta Pyza, Milena Damulewicz

The visual system is a sensory system which is sensitive to light and detects photic stimuli. It plays many important functions, such as vision, circadian clock entrainment and regulation of sleep-wake behavior. The interconnection between the visual system and clock network is precisely regulated. The outer layer of the visual system called the retina, is composed of opsin-based photoreceptors that, in addition to visual information, provide photic information for the circadian clock, which in turn, regulates daily rhythms, such as activity and sleep patterns. The retina houses its own circadian oscillators (belonging to peripheral oscillators), however, they are also controlled by the main clock (pacemaker). Photoreceptor cells show many clock and light-dependent rhythms, such as the rhythms in synaptic plasticity or rhodopsin turnover, but their precise regulation is still not completely understood. In this study, we provided evidence that one of the mechanisms involved in the regulation of retinal rhythms is autophagy. We showed that autophagy is rhythmic in photoreceptors, with a specific daily pattern of autophagosome levels in different cells. Moreover, our data suggest that rhythmic autophagy-dependent degradation of the presynaptic protein Bruchpilot or photosensitive rhodopsin is involved in the regulation of daily rhythms observed in the retina. In effect, autophagy disruption in the photoreceptors, which affects photic signal transmission to the main clock neurons, causes changes in sleep level and pattern.

视觉系统是一种感觉系统，它对光很敏感，能探测到光刺激。它具有许多重要的功能，如视觉、生物钟引导和睡眠-觉醒行为的调节。视觉系统和时钟网络之间的连接是精确调节的。视觉系统的外层称为视网膜，由视蛋白为基础的光感受器组成，除了视觉信息外，它还为昼夜节律钟提供光信息，昼夜节律钟反过来调节日常节律，如活动和睡眠模式。视网膜拥有自己的昼夜节律振荡器（属于外围振荡器），然而，它们也由主时钟（起搏器）控制。光感受器细胞表现出许多时钟和光依赖的节律，如突触可塑性或视紫红质更新的节律，但它们的精确调控尚不完全清楚。在这项研究中，我们提供了证据，其中一个机制参与视网膜节律的调节是自噬。我们发现自噬在光感受器中具有节律性，在不同细胞中具有特定的自噬体水平的每日模式。此外，我们的数据表明突触前蛋白Bruchpilot或光敏视紫质的节律性自噬依赖性降解参与了视网膜中观察到的日常节律调节。实际上，自噬破坏光感受器，影响光信号传递到主要时钟神经元，导致睡眠水平和模式的变化。

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引用次数: 0

Activation of the immune deficiency pathway (IMD) reduces the mosquito heart rate via a nitric oxide-based mechanism 免疫缺陷途径（IMD）的激活通过一种基于一氧化氮的机制降低蚊子的心率。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2024-12-06 DOI: 10.1016/j.jinsphys.2024.104738

Tania Y. Estévez-Lao, Lindsay E. Martin, Julián F. Hillyer

The immune deficiency pathway (IMD) is an important component of the antibacterial, antimalarial and antiviral response in mosquitoes. The IMD pathway also drives the infection induced migration of hemocytes to the heart. During an infection, periostial hemocytes kill pathogens in areas of high hemolymph flow and produce nitric oxide that reduces the heart rate. Here, we investigated the consequences of repressing the IMD pathway by silencing the transcription factor, rel2, or activating the pathway by silencing the negative regulator, caspar, in Anopheles gambiae. In uninfected mosquitoes, repression of the IMD pathway does not affect the circulatory system. However, activating the IMD pathway decreases the heart rate, and this correlates with increased transcription and activity of nitric oxide synthase (NOS), but not increased transcription of the lysozymes, LysC1 or LysC2. In infected mosquitoes, however, activation of the IMD pathway does not affect the heart rate but repression of the pathway decreases the heart rate. This latter phenotype correlates with increased transcription and activity of nitric oxide synthase, which is likely due to an increase in infection intensity. In conclusion, we demonstrate that a major immune signaling pathway that regulates periostial hemocyte aggregation, the IMD pathway, reduces the heart rate via a nitric oxide-based mechanism.

免疫缺陷途径（IMD）是蚊子抗菌、抗疟和抗病毒反应的重要组成部分。IMD途径也驱动感染诱导的血细胞向心脏迁移。在感染期间，骨膜血细胞杀死高血淋巴流量区域的病原体，并产生降低心率的一氧化氮。在冈比亚按蚊中，我们研究了通过沉默转录因子rel2或通过沉默负调节因子caspar激活IMD通路来抑制IMD通路的后果。在未感染的蚊子中，抑制IMD途径不影响循环系统。然而，激活IMD通路会降低心率，这与一氧化氮合酶（NOS）的转录和活性增加有关，而与溶菌酶LysC1或LysC2的转录增加无关。然而，在受感染的蚊子中，激活IMD途径不影响心率，但抑制该途径会降低心率。后一种表型与一氧化氮合酶转录和活性的增加相关，这可能是由于感染强度的增加。总之，我们证明了一个主要的免疫信号通路，调节外周血细胞聚集，IMD途径，通过一个基于一氧化氮的机制降低心率。

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引用次数: 0

Corticotropin-releasing factor-like diuretic hormone 44 and five corresponding GPCRs in Drosophila suzukii: Structural and functional characterization 苏氏果蝇促肾上腺皮质激素释放因子样利尿激素44和5个相应的gpcr：结构和功能表征。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2024-12-06 DOI: 10.1016/j.jinsphys.2024.104740

Hojung Yoon , Briana Price , Ryssa Parks , Hyo Sang Jang , Muhammad Hafeez , Jacob Corcoran , Seung-Joon Ahn , Man-Yeon Choi

Diuretic hormones (DHs) activate corresponding G protein-coupled receptors (GPCRs), mediating the water and ion homeostasis in arthropods. There are two different DHs known to be expressed in insects, calcitonin (CT)-like DH31 and corticotropin-releasing factor (CRF)-like DH44. In this study, we identified and characterized a DH44 and five GPCR variants, DH44-R1 and DH44-R2a/b/c/d, in Drosophila suzukii (spotted-wing drosophila), causing detrimental damage to fresh and soft-skinned fruits. Among the five DH44 receptors, DH44-R1 was the longest GPCR and most strongly responded to DH44, and the other DH44-R2 splicing variants were relatively shorter and over 90 % similar to each other. Some DH44-Rs including DH44-R1 utilized both cAMP and Ca²⁺ as second messengers. Interestingly, DH44-R1 was dominantly expressed in the brain, whereas DH44-R2 variants were dominant in the digestive organs, particularly the Malpighian tubules (MTs) by their gene expressions. The results suggest that DH44 may have multiple physiological functions, including the regulation of the sleep-wake cycle and diuretic activity. Injection of DH44 stimulated fluid secretion in adults, and the rate of the excretion increased in a dose-dependent manner. Moreover, when the flies were injected with a mixture of DH31 and DH44, a high mortality rate was observed. Here, we demonstrate the gene structures, expressions, characterization of DH44 and five GPCRs, their second messengers, and the effects of DH peptides on the fly. These investigations offer molecular insights into the physiological roles of the DH system and may assist in the fundamental aspects of developing D. suzukii management in the field.

利尿激素（Diuretic hormone, DHs）激活相应的G蛋白偶联受体（gpcr），介导节肢动物体内水分和离子的稳态。已知昆虫中表达两种不同的DHs，降钙素（CT）样DH31和促肾上腺皮质激素释放因子（CRF）样DH44。在这项研究中，我们鉴定并鉴定了一个DH44和五个GPCR变异DH44- r1和DH44- r2a /b/c/d，对新鲜和软皮果蝇造成有害损害。在5个DH44受体中，DH44- r1是最长的GPCR，对DH44的应答最强烈，其他DH44- r2剪接变异体相对较短，相似度在90% %以上。包括DH44-R1在内的一些DH44-Rs同时利用cAMP和Ca2+作为第二信使。有趣的是，DH44-R1主要在大脑中表达，而DH44-R2变体主要在消化器官中表达，特别是马尔比氏小管（MTs）。结果表明，DH44可能具有多种生理功能，包括调节睡眠-觉醒周期和利尿活性。注射DH44刺激成人体液分泌，排泄率呈剂量依赖性增加。此外，DH31和DH44混合注射后，果蝇死亡率较高。在这里，我们展示了DH44和五种gpcr及其第二信使的基因结构、表达、特征，以及DH肽对果蝇的影响。这些研究为DH系统的生理作用提供了分子见解，并可能有助于在该领域发展铃木氏菌管理的基本方面。

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引用次数: 0

Effects of temperature and humidity on the survival and hatching response of diapausing and non-diapausing Aedes aegypti eggs 温度和湿度对滞育和非滞育埃及伊蚊卵存活和孵化反应的影响。

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2024-12-03 DOI: 10.1016/j.jinsphys.2024.104726

Sylvia Fischer , María Sol De Majo , Cristian Di Battista , Raúl E. Campos

In seasonally varying environments, diapause, which is induced by a short photoperiod, favors overwintering of many insects. In Aedine mosquitoes, embryonic diapause is associated with higher survival and resistance to low temperature and humidity. Aedes aegypti, the main vector of dengue and other arboviruses, has recently expanded its distribution towards temperate regions. One of the mechanisms that might have favored this expansion in South America is the ability to induce embryonic diapause. This type of diapause has been recently discovered in populations from Argentina, associated with hatching inhibition and increased amounts of lipids in the eggs. The aim of this study was to assess the four-month survival of diapausing (D) and non-diapausing (ND) eggs stored at different humidity and temperature conditions. Two populations from the temperate region of Argentina were analyzed: one from Buenos Aires (BA), a city with a relatively mild and short winter, and another from San Bernardo (SB), a locality with a harsher and longer winter. For both populations, D and ND eggs were obtained from colonies maintained under 10:14 L:D and 14:10 L:D hours respectively. Eggs were exposed to six different conditions of humidity and temperature for 85 days. After exposure, egg survival and hatching response were analyzed. D eggs showed significantly higher survival at low humidity (both populations), and at medium and high humidity and at low temperatures (SB population). In addition, D eggs showed a significantly lower hatching response at high humidity and low temperatures, and higher proportion of not hatched eggs remaining viable after two immersions under all conditions. D eggs from SB were significantly more tolerant to low temperatures than those from BA. ND eggs from SB were significantly more tolerant to low temperatures, while those from BA were more tolerant to low humidity. Overall, the effect of diapause was a significant increase in the number of not hatched, viable embryos after immersion. Results suggest that the ability of Ae. aegypti to induce egg diapause increases the probability of successful overwintering and further expansion of its distribution range, and as a consequence the risk of arbovirus transmission might increase in temperate areas.

在季节变化的环境中，由较短的光周期引起的滞育有利于许多昆虫越冬。在伊蚊中，胚胎滞育与更高的存活率和对低温和低湿度的抵抗力有关。登革热和其他虫媒病毒的主要媒介埃及伊蚊最近向温带地区扩大了分布。其中一个可能有利于南美洲这种扩张的机制是诱导胚胎滞育的能力。最近在阿根廷的种群中发现了这种类型的滞育，这与孵化抑制和卵中脂质的增加有关。本研究的目的是评估不同湿度和温度条件下滞育(D)和非滞育（ND）卵的4个月存活率。研究分析了阿根廷温带地区的两个人群：一个来自布宜诺斯艾利斯（BA），一个冬天相对温和和短暂的城市，另一个来自圣贝纳多（SB），一个冬天更严酷和更长的地方。两个种群分别在10:14 L:D和14:10 L:D维持的菌落中获得D和ND卵。鸡蛋暴露在六种不同的湿度和温度条件下85 天。暴露后，分析卵存活率和孵化反应。D卵在低湿度（2个种群）和中、高湿及低温条件下（SB种群）的成活率显著提高。此外，在高湿低温条件下，D卵的孵化响应显著降低，2次浸泡后未孵化卵的存活率均较高。SB的D蛋对低温的耐受性显著高于BA的D蛋。来自SB的ND蛋对低温的耐受性显著提高，而来自BA的ND蛋对低湿的耐受性显著提高。总的来说，滞育的影响是浸泡后未孵化的活胚数量显著增加。结果表明，Ae。埃及伊蚊诱导卵滞育增加了成功越冬的可能性，并进一步扩大了其分布范围，因此在温带地区虫媒病毒传播的风险可能增加。

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引用次数: 0

Corrigendum to “Cellular dynamics of host − parasitoid interactions: Insights from the encapsulation process in a partially resistant host” [J. Insect Physiol. 155 (2024) 104646] 寄主与寄生虫相互作用的细胞动力学：寄主-寄生虫相互作用的细胞动力学：从部分抗性寄主的封装过程中获得的启示" [J. Insect Physiol.

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology

Pub Date : 2024-12-01 DOI: 10.1016/j.jinsphys.2024.104711

Samuel Gornard, Florence Mougel, Isabelle Germon, Véronique Borday-Birraux, Pascaline Venon, Salimata Drabo, Laure Kaiser

引用次数: 0