High-resolution transmission electron microscopy (HRTEM) images can capture the atomic-resolution details of the dynamically changing structure of nanomaterials. Here, we propose a new scheme and an improved reconstruction algorithm to reconstruct the exit wave function for each image in a focal series of HRTEM images to reveal structural changes. In this scheme, the wave reconstructed from the focal series of images is treated as the initial wave in the reconstruction process for each HRTEM image. Additionally, to suppress noise at the frequencies where the signal is weak due to the modulation of the lens transfer function, a weight factor is introduced in the improved reconstruction algorithm. The advantages of the new scheme and algorithms are validated by using the HRTEM images of a natural specimen and a single-layer molybdenum disulphide. This algorithm enables image resolution enhancement and lens aberration removal, while potentially allowing the visualisation of the structural evolution of nanostructures.
{"title":"Exit wave reconstruction of a focal series of images with structural changes in high-resolution transmission electron microscopy","authors":"Xiaohan Zhang, Shaowen Chen, Shuya Wang, Ying Huang, Chuanhong Jin, Fang Lin","doi":"10.1111/jmi.13335","DOIUrl":"10.1111/jmi.13335","url":null,"abstract":"<p>High-resolution transmission electron microscopy (HRTEM) images can capture the atomic-resolution details of the dynamically changing structure of nanomaterials. Here, we propose a new scheme and an improved reconstruction algorithm to reconstruct the exit wave function for each image in a focal series of HRTEM images to reveal structural changes. In this scheme, the wave reconstructed from the focal series of images is treated as the initial wave in the reconstruction process for each HRTEM image. Additionally, to suppress noise at the frequencies where the signal is weak due to the modulation of the lens transfer function, a weight factor is introduced in the improved reconstruction algorithm. The advantages of the new scheme and algorithms are validated by using the HRTEM images of a natural specimen and a single-layer molybdenum disulphide. This algorithm enables image resolution enhancement and lens aberration removal, while potentially allowing the visualisation of the structural evolution of nanostructures.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"296 1","pages":"24-33"},"PeriodicalIF":1.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bugnet, S. Löffler, M. Ederer, D. M. Kepaptsoglou, Q. M. Ramasse
The concept of electronic orbitals has enabled the understanding of a wide range of physical and chemical properties of solids through the definition of, for example, chemical bonding between atoms. In the transmission electron microscope, which is one of the most used and powerful analytical tools for high-spatial-resolution analysis of solids, the accessible quantity is the local distribution of electronic states. However, the interpretation of electronic state maps at atomic resolution in terms of electronic orbitals is far from obvious, not always possible, and often remains a major hurdle preventing a better understanding of the properties of the system of interest. In this review, the current state of the art of the experimental aspects for electronic state mapping and its interpretation as electronic orbitals is presented, considering approaches that rely on elastic and inelastic scattering, in real and reciprocal spaces. This work goes beyond resolving spectral variations between adjacent atomic columns, as it aims at providing deeper information about, for example, the spatial or momentum distributions of the states involved. The advantages and disadvantages of existing experimental approaches are discussed, while the challenges to overcome and future perspectives are explored in an effort to establish the current state of knowledge in this field. The aims of this review are also to foster the interest of the scientific community and to trigger a global effort to further enhance the current analytical capabilities of transmission electron microscopy for chemical bonding and electronic structure analysis.
{"title":"Current opinion on the prospect of mapping electronic orbitals in the transmission electron microscope: State of the art, challenges and perspectives","authors":"M. Bugnet, S. Löffler, M. Ederer, D. M. Kepaptsoglou, Q. M. Ramasse","doi":"10.1111/jmi.13321","DOIUrl":"10.1111/jmi.13321","url":null,"abstract":"<p>The concept of electronic orbitals has enabled the understanding of a wide range of physical and chemical properties of solids through the definition of, for example, chemical bonding between atoms. In the transmission electron microscope, which is one of the most used and powerful analytical tools for high-spatial-resolution analysis of solids, the accessible quantity is the local distribution of electronic states. However, the interpretation of electronic state maps at atomic resolution in terms of electronic orbitals is far from obvious, not always possible, and often remains a major hurdle preventing a better understanding of the properties of the system of interest. In this review, the current state of the art of the experimental aspects for electronic state mapping and its interpretation as electronic orbitals is presented, considering approaches that rely on elastic and inelastic scattering, in real and reciprocal spaces. This work goes beyond resolving spectral variations between adjacent atomic columns, as it aims at providing deeper information about, for example, the spatial or momentum distributions of the states involved. The advantages and disadvantages of existing experimental approaches are discussed, while the challenges to overcome and future perspectives are explored in an effort to establish the current state of knowledge in this field. The aims of this review are also to foster the interest of the scientific community and to trigger a global effort to further enhance the current analytical capabilities of transmission electron microscopy for chemical bonding and electronic structure analysis.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"295 3","pages":"217-235"},"PeriodicalIF":1.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ziao Jiao, Mingliang Pan, Khadija Yousaf, Daniel Doveiko, Michelle Maclean, David Griffin, Yu Chen, David Day Uei Li
We propose a smartphone-based optical sectioning (SOS) microscope based on the HiLo technique, with a single smartphone replacing a high-cost illumination source and a camera sensor. We built our SOS with off-the-shelf optical, mechanical cage systems with 3D-printed adapters to seamlessly integrate the smartphone with the SOS main body. The liquid light guide can be integrated with the adapter, guiding the smartphone's LED light to the digital mirror device (DMD) with neglectable loss. We used an electrically tuneable lens (ETL) instead of a mechanical translation stage to realise low-cost axial scanning. The ETL was conjugated to the objective lens's back pupil plane (BPP) to construct a telecentric design by a 4f configuration to maintain the lateral magnification for different axial positions. SOS has a 571.5 µm telecentric scanning range and an 11.7 µm axial resolution. The broadband smartphone LED torch can effectively excite fluorescent polystyrene (PS) beads. We successfully used SOS for high-contrast fluorescent PS beads imaging with different wavelengths and optical sectioning imaging of multilayer fluorescent PS beads. To our knowledge, the proposed SOS is the first smartphone-based HiLo optical sectioning microscopy (£1965), which can save around £7035 compared with a traditional HiLo system (£9000). It is a powerful tool for biomedical research in resource-limited areas.
我们提出了一种基于智能手机的光学切片(SOS)显微镜,它以 HiLo 技术为基础,用一部智能手机取代了高成本的照明光源和相机传感器。我们利用现成的光学、机械笼系统和 3D 打印适配器构建了 SOS,将智能手机与 SOS 主体无缝集成。液态光导可与适配器集成,以可忽略的损耗将智能手机的 LED 光导至数字镜像设备(DMD)。我们使用电动可调透镜(ETL)代替机械平移台,以实现低成本的轴向扫描。ETL 与物镜的后瞳孔平面 (BPP) 连接,通过 4f 配置构建远心设计,以保持不同轴向位置的横向放大率。SOS 的远心扫描范围为 571.5 微米,轴向分辨率为 11.7 微米。宽带智能手机 LED 手电筒可有效激发荧光聚苯乙烯(PS)珠。我们成功地将 SOS 用于不同波长的高对比度荧光聚苯乙烯珠成像和多层荧光聚苯乙烯珠的光学切片成像。据我们所知,拟议的 SOS 是首个基于智能手机的 HiLo 光学切片显微镜(1965 英镑),与传统 HiLo 系统(9000 英镑)相比,可节省约 7035 英镑。它是资源有限地区生物医学研究的有力工具。
{"title":"Smartphone-based optical sectioning (SOS) microscopy with a telecentric design for fluorescence imaging","authors":"Ziao Jiao, Mingliang Pan, Khadija Yousaf, Daniel Doveiko, Michelle Maclean, David Griffin, Yu Chen, David Day Uei Li","doi":"10.1111/jmi.13334","DOIUrl":"10.1111/jmi.13334","url":null,"abstract":"<p>We propose a smartphone-based optical sectioning (SOS) microscope based on the HiLo technique, with a single smartphone replacing a high-cost illumination source and a camera sensor. We built our SOS with off-the-shelf optical, mechanical cage systems with 3D-printed adapters to seamlessly integrate the smartphone with the SOS main body. The liquid light guide can be integrated with the adapter, guiding the smartphone's LED light to the digital mirror device (DMD) with neglectable loss. We used an electrically tuneable lens (ETL) instead of a mechanical translation stage to realise low-cost axial scanning. The ETL was conjugated to the objective lens's back pupil plane (BPP) to construct a telecentric design by a 4f configuration to maintain the lateral magnification for different axial positions. SOS has a 571.5 µm telecentric scanning range and an 11.7 µm axial resolution. The broadband smartphone LED torch can effectively excite fluorescent polystyrene (PS) beads. We successfully used SOS for high-contrast fluorescent PS beads imaging with different wavelengths and optical sectioning imaging of multilayer fluorescent PS beads. To our knowledge, the proposed SOS is the first smartphone-based HiLo optical sectioning microscopy (£1965), which can save around £7035 compared with a traditional HiLo system (£9000). It is a powerful tool for biomedical research in resource-limited areas.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"296 1","pages":"10-23"},"PeriodicalIF":1.5,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13334","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Jaime, S. Desbief, J. Silvent, G. Goupil, M. Bernacki, N. Bozzolo, A. Nicolaÿ
The curtaining effect is a common challenge in focused ion beam (FIB) surface preparation. This study investigates methods to reduce this effect during plasma FIB milling of Inconel 718 (nickel-based superalloy). Platinum deposition, silicon mask and XeF2 gas injection were explored as potential solutions. These methods were evaluated for two ion beam current conditions; a high ion beam intensity condition (30 kV–1 µA) and a medium one (30 kV–100 nA) and their impact on curtaining reduction and resulting cross-section quality was assessed quantitatively thanks to topographic measurements done by atomic force microscopy (AFM). XeF2 assistance notably improved cross-section quality at medium current level. Pt deposition and Si mask individually mitigated the curtaining effect, with greater efficacy at 100 nA. Both methods also contributed to reducing cross-section curvature, with the Si mask outperforming Pt deposition. However, combining Pt deposition and Si mask with XeF2 injection led to deterioration of these protective layers and the reappearance of the curtaining effect after a quite short exposure time.
{"title":"Study of curtaining effect reduction methods in Inconel 718 using a plasma focused ion beam","authors":"F. Jaime, S. Desbief, J. Silvent, G. Goupil, M. Bernacki, N. Bozzolo, A. Nicolaÿ","doi":"10.1111/jmi.13320","DOIUrl":"10.1111/jmi.13320","url":null,"abstract":"<p>The curtaining effect is a common challenge in focused ion beam (FIB) surface preparation. This study investigates methods to reduce this effect during plasma FIB milling of Inconel 718 (nickel-based superalloy). Platinum deposition, silicon mask and XeF<sub>2</sub> gas injection were explored as potential solutions. These methods were evaluated for two ion beam current conditions; a high ion beam intensity condition (30 kV–1 µA) and a medium one (30 kV–100 nA) and their impact on curtaining reduction and resulting cross-section quality was assessed quantitatively thanks to topographic measurements done by atomic force microscopy (AFM). XeF<sub>2</sub> assistance notably improved cross-section quality at medium current level. Pt deposition and Si mask individually mitigated the curtaining effect, with greater efficacy at 100 nA. Both methods also contributed to reducing cross-section curvature, with the Si mask outperforming Pt deposition. However, combining Pt deposition and Si mask with XeF<sub>2</sub> injection led to deterioration of these protective layers and the reappearance of the curtaining effect after a quite short exposure time.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"295 3","pages":"287-299"},"PeriodicalIF":1.5,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christian Schmidt, Tom Boissonnet, Julia Dohle, Karen Bernhardt, Elisa Ferrando-May, Tobias Wernet, Roland Nitschke, Susanne Kunis, Stefanie Weidtkamp-Peters
Bioimage data are generated in diverse research fields throughout the life and biomedical sciences. Its potential for advancing scientific progress via modern, data-driven discovery approaches reaches beyond disciplinary borders. To fully exploit this potential, it is necessary to make bioimaging data, in general, multidimensional microscopy images and image series, FAIR, that is, findable, accessible, interoperable and reusable. These FAIR principles for research data management are now widely accepted in the scientific community and have been adopted by funding agencies, policymakers and publishers. To remain competitive and at the forefront of research, implementing the FAIR principles into daily routines is an essential but challenging task for researchers and research infrastructures. Imaging core facilities, well-established providers of access to imaging equipment and expertise, are in an excellent position to lead this transformation in bioimaging research data management. They are positioned at the intersection of research groups, IT infrastructure providers, the institution´s administration, and microscope vendors. In the frame of German BioImaging – Society for Microscopy and Image Analysis (GerBI-GMB), cross-institutional working groups and third-party funded projects were initiated in recent years to advance the bioimaging community's capability and capacity for FAIR bioimage data management. Here, we provide an imaging-core-facility-centric perspective outlining the experience and current strategies in Germany to facilitate the practical adoption of the FAIR principles closely aligned with the international bioimaging community. We highlight which tools and services are ready to be implemented and what the future directions for FAIR bioimage data have to offer.
{"title":"A practical guide to bioimaging research data management in core facilities","authors":"Christian Schmidt, Tom Boissonnet, Julia Dohle, Karen Bernhardt, Elisa Ferrando-May, Tobias Wernet, Roland Nitschke, Susanne Kunis, Stefanie Weidtkamp-Peters","doi":"10.1111/jmi.13317","DOIUrl":"10.1111/jmi.13317","url":null,"abstract":"<p>Bioimage data are generated in diverse research fields throughout the life and biomedical sciences. Its potential for advancing scientific progress via modern, data-driven discovery approaches reaches beyond disciplinary borders. To fully exploit this potential, it is necessary to make bioimaging data, in general, multidimensional microscopy images and image series, FAIR, that is, findable, accessible, interoperable and reusable. These FAIR principles for research data management are now widely accepted in the scientific community and have been adopted by funding agencies, policymakers and publishers. To remain competitive and at the forefront of research, implementing the FAIR principles into daily routines is an essential but challenging task for researchers and research infrastructures. Imaging core facilities, well-established providers of access to imaging equipment and expertise, are in an excellent position to lead this transformation in bioimaging research data management. They are positioned at the intersection of research groups, IT infrastructure providers, the institution´s administration, and microscope vendors. In the frame of German BioImaging – Society for Microscopy and Image Analysis (GerBI-GMB), cross-institutional working groups and third-party funded projects were initiated in recent years to advance the bioimaging community's capability and capacity for FAIR bioimage data management. Here, we provide an imaging-core-facility-centric perspective outlining the experience and current strategies in Germany to facilitate the practical adoption of the FAIR principles closely aligned with the international bioimaging community. We highlight which tools and services are ready to be implemented and what the future directions for FAIR bioimage data have to offer.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"294 3","pages":"350-371"},"PeriodicalIF":2.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13317","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariana De Niz, Rodrigo Escobedo García, Celina Terán Ramirez, Ysa Pakowski, Yuriney Abonza, Nikki Bialy, Vanessa L. Orr, Andres Olivera, Victor Abonza, Karina Alleva, Silvana Allodi, Michael F. Almeida, Alexis Ricardo Becerril Cuevas, Frederic Bonnet, Armando Burgos Solorio, Teng-Leong Chew, Gustavo Chiabrando, Beth Cimini, Aurélie Cleret-Buhot, Gastón Contreras Jiménez, Laura Daza, Vanessa De Sá, Natalia De Val, Diego L. Delgado-Álvarez, Kevin Eliceiri, Reto Fiolka, Hernan Grecco, Dorit Hanein, Paúl Hernández Herrera, Phil Hockberger, Haydee O. Hernandez, Yael Hernandez Guadarrama, Michelle Itano, Caron A. Jacobs, Luis F. Jiménez-García, Vilma Jiménez Sabinina, Andres Kamaid, Antje Keppler, Abhishek Kumar, Judith Lacoste, Alenka Lovy, Kate Luby-Phelps, Anita Mahadevan-Jansen, Leonel Malacrida, Shalin B. Mehta, Caroline Miller, Kildare Miranda, Joshua A. Moore, Alison North, Peter O'Toole, Mariana Olivares Urbano, Lía I. Pietrasanta, Rodrigo V. Portugal, Andrés H. Rossi, Jonathan Sanchez Contreras, Caterina Strambio-De-Castilla, Gloria Soldevila, Bruno Vale, Diana Vazquez, Chris Wood, Claire M. Brown, Adan Guerrero
In September 2023, the two largest bioimaging networks in the Americas, Latin America Bioimaging (LABI) and BioImaging North America (BINA), came together during a 1-week meeting in Mexico. This meeting provided opportunities for participants to interact closely with decision-makers from imaging core facilities across the Americas. The meeting was held in a hybrid format and attended in-person by imaging scientists from across the Americas, including Canada, the United States, Mexico, Colombia, Peru, Argentina, Chile, Brazil and Uruguay. The aims of the meeting were to discuss progress achieved over the past year, to foster networking and collaborative efforts among members of both communities, to bring together key members of the international imaging community to promote the exchange of experience and expertise, to engage with industry partners, and to establish future directions within each individual network, as well as common goals. This meeting report summarises the discussions exchanged, the achievements shared, and the goals set during the LABIxBINA2023: Bioimaging across the Americas meeting.
{"title":"Building momentum through networks: Bioimaging across the Americas","authors":"Mariana De Niz, Rodrigo Escobedo García, Celina Terán Ramirez, Ysa Pakowski, Yuriney Abonza, Nikki Bialy, Vanessa L. Orr, Andres Olivera, Victor Abonza, Karina Alleva, Silvana Allodi, Michael F. Almeida, Alexis Ricardo Becerril Cuevas, Frederic Bonnet, Armando Burgos Solorio, Teng-Leong Chew, Gustavo Chiabrando, Beth Cimini, Aurélie Cleret-Buhot, Gastón Contreras Jiménez, Laura Daza, Vanessa De Sá, Natalia De Val, Diego L. Delgado-Álvarez, Kevin Eliceiri, Reto Fiolka, Hernan Grecco, Dorit Hanein, Paúl Hernández Herrera, Phil Hockberger, Haydee O. Hernandez, Yael Hernandez Guadarrama, Michelle Itano, Caron A. Jacobs, Luis F. Jiménez-García, Vilma Jiménez Sabinina, Andres Kamaid, Antje Keppler, Abhishek Kumar, Judith Lacoste, Alenka Lovy, Kate Luby-Phelps, Anita Mahadevan-Jansen, Leonel Malacrida, Shalin B. Mehta, Caroline Miller, Kildare Miranda, Joshua A. Moore, Alison North, Peter O'Toole, Mariana Olivares Urbano, Lía I. Pietrasanta, Rodrigo V. Portugal, Andrés H. Rossi, Jonathan Sanchez Contreras, Caterina Strambio-De-Castilla, Gloria Soldevila, Bruno Vale, Diana Vazquez, Chris Wood, Claire M. Brown, Adan Guerrero","doi":"10.1111/jmi.13318","DOIUrl":"10.1111/jmi.13318","url":null,"abstract":"<p>In September 2023, the two largest bioimaging networks in the Americas, Latin America Bioimaging (LABI) and BioImaging North America (BINA), came together during a 1-week meeting in Mexico. This meeting provided opportunities for participants to interact closely with decision-makers from imaging core facilities across the Americas. The meeting was held in a hybrid format and attended in-person by imaging scientists from across the Americas, including Canada, the United States, Mexico, Colombia, Peru, Argentina, Chile, Brazil and Uruguay. The aims of the meeting were to discuss progress achieved over the past year, to foster networking and collaborative efforts among members of both communities, to bring together key members of the international imaging community to promote the exchange of experience and expertise, to engage with industry partners, and to establish future directions within each individual network, as well as common goals. This meeting report summarises the discussions exchanged, the achievements shared, and the goals set during the LABIxBINA2023: Bioimaging across the Americas meeting.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"294 3","pages":"420-439"},"PeriodicalIF":2.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13318","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arbuscular mycorrhizal (AM) symbiosis, the nutritional partnership between AM fungi and most plant species, is globally ubiquitous and of great ecological and agricultural importance. Studying the processes of AM symbiosis is confounded by its highly spatiotemporally dynamic nature. While microscopy methods exist to probe the spatial side of this plant-fungal interaction, the temporal side remains more challenging, as reliable deep-tissue time-lapse imaging requires both symbiotic partners to remain undisturbed over prolonged time periods. Here, we introduce the AMSlide: a noninvasive, high-resolution, live-imaging system optimised for AM symbiosis research. We demonstrate the AMSlide's applications in confocal microscopy of mycorrhizal roots, from whole colonisation zones to subcellular structures, over timeframes from minutes to weeks. The AMSlide's versatility for different microscope set-ups, imaging techniques, and plant and fungal species is also outlined. It is hoped that the AMSlide will be applied in future research to fill in the temporal blanks in our understanding of AM symbiosis, as well as broader root and rhizosphere processes.
丛枝菌根(AM)共生是 AM 真菌与大多数植物物种之间的营养合作关系,在全球无处不在,具有重要的生态和农业意义。研究 AM 共生的过程受到其高度时空动态性质的困扰。虽然已有显微镜方法可以探测这种植物-真菌相互作用的空间方面,但时间方面仍然更具挑战性,因为可靠的深组织延时成像要求共生双方在长时间内保持不受干扰。在这里,我们介绍 AMSlide:一种非侵入式、高分辨率、实时成像系统,是 AM 共生研究的最佳选择。我们展示了 AMSlide 在菌根共聚焦显微镜中的应用,从整个定植区到亚细胞结构,时间范围从几分钟到几周不等。此外,还概述了 AMSlide 在不同显微镜设置、成像技术以及植物和真菌物种方面的多功能性。希望 AMSlide 能在未来的研究中得到应用,以填补我们对 AM 共生以及更广泛的根部和根圈过程的认识上的时间空白。
{"title":"The AMSlide for noninvasive time-lapse imaging of arbuscular mycorrhizal symbiosis.","authors":"Jennifer McGaley, Ben Schneider, Uta Paszkowski","doi":"10.1111/jmi.13313","DOIUrl":"https://doi.org/10.1111/jmi.13313","url":null,"abstract":"<p><p>Arbuscular mycorrhizal (AM) symbiosis, the nutritional partnership between AM fungi and most plant species, is globally ubiquitous and of great ecological and agricultural importance. Studying the processes of AM symbiosis is confounded by its highly spatiotemporally dynamic nature. While microscopy methods exist to probe the spatial side of this plant-fungal interaction, the temporal side remains more challenging, as reliable deep-tissue time-lapse imaging requires both symbiotic partners to remain undisturbed over prolonged time periods. Here, we introduce the AMSlide: a noninvasive, high-resolution, live-imaging system optimised for AM symbiosis research. We demonstrate the AMSlide's applications in confocal microscopy of mycorrhizal roots, from whole colonisation zones to subcellular structures, over timeframes from minutes to weeks. The AMSlide's versatility for different microscope set-ups, imaging techniques, and plant and fungal species is also outlined. It is hoped that the AMSlide will be applied in future research to fill in the temporal blanks in our understanding of AM symbiosis, as well as broader root and rhizosphere processes.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Core facilities coming of age","authors":"Sebastian Munck, Kurt I. Anderson","doi":"10.1111/jmi.13319","DOIUrl":"10.1111/jmi.13319","url":null,"abstract":"","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"294 3","pages":"253-254"},"PeriodicalIF":2.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Light microscopy facilities vary in the number of imaging systems and the scope of technologies they support. Each facility must craft an identity through the selection of equipment and development of staff in order to serve the needs of its local research environment. The process of crafting a light microscopy facility can be compared to curation of an art exhibition: great care should be given to the selection and placement of each object in order to make a coherent statement.
Lay Description: Light microscopy facilities vary in the number of imaging systems and the scope of technologies they support. Each facility must develop an identity through the selection of equipment and development of staff in order to serve the needs of its local research environment. The process of crafting a light microscopy facility can be compared to curation of an art exhibition: great care should be given to the selection and placement of each object in order to make a coherent statement.
{"title":"Strategies for selecting and managing equipment in a light microscopy facility","authors":"Kurt I. Anderson","doi":"10.1111/jmi.13316","DOIUrl":"10.1111/jmi.13316","url":null,"abstract":"<p>Light microscopy facilities vary in the number of imaging systems and the scope of technologies they support. Each facility must craft an identity through the selection of equipment and development of staff in order to serve the needs of its local research environment. The process of crafting a light microscopy facility can be compared to curation of an art exhibition: great care should be given to the selection and placement of each object in order to make a coherent statement.</p><p><b>Lay Description</b>: Light microscopy facilities vary in the number of imaging systems and the scope of technologies they support. Each facility must develop an identity through the selection of equipment and development of staff in order to serve the needs of its local research environment. The process of crafting a light microscopy facility can be compared to curation of an art exhibition: great care should be given to the selection and placement of each object in order to make a coherent statement.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"294 3","pages":"268-275"},"PeriodicalIF":2.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13316","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}