Journal of Obstetrics and Gynaecology Research最新文献_第9页

CD1a affects the recurrence and prognosis of ovarian cancer CD1a 影响卵巢癌的复发和预后。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-07 DOI: 10.1111/jog.16120

Qiong Zhu, Jun Liu, Yinghao Xie, Chengqiu Wu

Objective

Explored the correlation between CD1a expression in recurrence and prognosis of ovarian cancer (OV).

Methods

The CD1a expression profile in OV, recurrent OV, and normal tissues, as well as corresponding clinical data, were obtained from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC), Gene Expression Omnibus (GEO), and Genotype Tissue Expression (GTEx) databases. Meanwhile, immunohistochemical detection of CD1a expression in normal and OV tissues. Kaplan–Meier curves were plotted to estimate the hazard ratio (HR) of survival in OV. In addition, the correlation between CD1a and immune cells in OV, as well as the CD1a expression profile and corresponding survival time in pan-cancer were obtained from TCGA database.

Results

CD1a was overexpressed in OV and was significantly under-expressed in recurrent OV (TCGA-OV, p < 0.0001 and ICGC-OV, p < 0.0001). CD1a immunohistochemistry is significantly overexpressed in OV compared to normal tissue (p < 0.05). Recurrent OV (ICGC, p < 0.001; GSE17260, p < 0.001; GSE32062, p < 0.05). The prognosis in OV was significantly better when CD1a is overexpressed compared to under-expressed (HR [low], 1.426: 95% confidence interval [CI], 0.912–2.128; p = 0.050). Meanwhile, the overexpression of CD1a has a better prognosis than low expression in OV and recurrent OV (p = 0.004, HR [low] = 2.462, 95%CI [1.346–4.504] and p = 0.011, HR [low] = 2.199, 95%CI [1.202–4.024]). In addition, CD1a expression was closely correlated with immune cells, the CD8+ T cells, macrophages, and NK cells, while uncharacterized cells were significantly different (p = 2.65e−6, p = 7.52e−13, p = 8.28e−12, and p = 5.89e−8, respectively). Moreover, CD1a expression affected the prognosis in various other cancers.

Conclusions

CD1a expression affected the recurrence and prognosis of OV and is closely related to various immune cell levels.

目的：探讨 CD1a 表达与卵巢癌（OV）复发和预后的相关性：探讨CD1a表达在卵巢癌（OV）复发和预后中的相关性：方法：从The Cancer Genome Atlas（TCGA）、International Cancer Genome Consortium（ICGC）、Gene Expression Omnibus（GEO）和Genotype Tissue Expression（GTEx）数据库中获取卵巢癌、复发性卵巢癌和正常组织中CD1a的表达谱以及相应的临床数据。同时，免疫组化检测CD1a在正常组织和OV组织中的表达。绘制 Kaplan-Meier 曲线以估算 OV 的生存危险比（HR）。此外，还从TCGA数据库中获得了OV中CD1a与免疫细胞的相关性，以及泛癌中CD1a的表达谱和相应的生存时间：结果：CD1a在OV中表达过高，在复发性OV中表达明显偏低（TCGA-OV，p 结论：CD1a的表达影响OV的复发率：CD1a的表达影响OV的复发和预后，并与各种免疫细胞水平密切相关。

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引用次数: 0

Impact of uterine cavity filling pressure levels on pain and procedure duration in diagnostic hysteroscopy: A retrospective cohort study 宫腔充盈压力水平对诊断性宫腔镜检查中疼痛和手术持续时间的影响：一项回顾性队列研究。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-07 DOI: 10.1111/jog.16146

Can Tercan, Emrah Dagdeviren, Ali Selcuk Yeniocak, Sultan Can, Burak Yucel

Aim

This study investigates the impact of intracavitary pressure levels on pain, visualization quality, and procedure duration in office hysteroscopy, comparing standard pressures (60–100 mmHg) with high pressures (110–150 mmHg).

Methods

We conducted a retrospective cohort study involving non-menopausal women undergoing diagnostic hysteroscopy at a tertiary clinic. Patients were categorized into two groups based on intracavitary pressure: the standard pressure group (60–100 mmHg; n = 72) and the high-pressure group (110–150 mmHg; n = 37). Outcomes measured included pain scores, procedure duration, and fluid volume used. Logistic regression was employed to identify risk factors for severe pain.

Results

The high-pressure group demonstrated significantly shorter procedure durations compared to the standard pressure group (96.56 ± 34.36 vs. 163.00 ± 61.78 s, p < 0.001). Pain scores were lower in the high-pressure group (VAS 3.00 [0.00–7.00] vs. 3.50 [0.00–9.00], p = 0.041). Additionally, high-pressure procedures used less fluid compared to standard pressure procedures, which helps to reduce the risk of fluid-related complications. Logistic regression analysis identified higher fluid volumes (odds ratio [OR] = 1.005, 95% confidence interval [CI] = 1.001–1.010) and the need for pressure increases (OR = 3.914, 95% CI = 1.157–13.238) as significant risk factors for severe pain.

Conclusions

Higher intracavitary pressure levels (above 100 mmHg) in office hysteroscopy are associated with reduced pain, shorter procedure durations, and decreased fluid use, enhancing visualization and procedural efficiency while maintaining patient comfort.

目的：本研究比较了标准压力（60-100 mmHg）和高压（110-150 mmHg），探讨了腔内压力水平对诊室宫腔镜检查中疼痛、可视化质量和手术持续时间的影响：我们进行了一项回顾性队列研究，研究对象是在一家三级医院接受诊断性宫腔镜检查的非绝经妇女。根据腔内压力将患者分为两组：标准压力组（60-100 mmHg；n = 72）和高压组（110-150 mmHg；n = 37）。测量结果包括疼痛评分、手术持续时间和所用液体量。采用逻辑回归法确定严重疼痛的风险因素：结果：与标准压力组相比，高压组的手术持续时间明显更短（96.56 ± 34.36 vs. 163.00 ± 61.78 秒，P 结论：高压组的手术持续时间明显更短，而标准压力组的手术持续时间明显更长（96.56 ± 34.36 vs. 163.00 ± 61.78 秒，P 结论）：诊室宫腔镜检查中较高的腔内压力水平（高于 100 mmHg）与减轻疼痛、缩短手术时间和减少液体用量有关，在保持患者舒适度的同时提高了可视化和手术效率。

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引用次数: 0

Association of maternal 25(OH)D levels during pregnancy with fetal birth weight and preschooler growth status: A retrospective birth cohort study 孕期母体 25（OH）D 水平与胎儿出生体重和学龄前儿童生长状况的关系：一项回顾性出生队列研究。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-06 DOI: 10.1111/jog.16143

Lan Yang, Zhengjia Ling, Di Yao, Jingna Su, Shufeng He, Xiaopei Zhou, Ying Gu

Aim

There is limited and conflicting evidence available regarding the correlation between maternal vitamin D status and childhood overweight and body mass index (BMI). The aim of this study was to investigate the following: (1) potential association between maternal 25-hydroxyvitamin D (25(OH)D) levels and newborn growth status; (2) relationship between maternal 25OHD levels and BMI and the risk of overweight with preschoolers being overweight.

Methods

A cohort of 3213 eligible singleton mother–infant pairs were used to investigate the possible associations between maternal 25(OH)D levels and fetal growth status. Data of 1767 available singleton mother–infant pairs and 6-year-old preschoolers was applied to analyze the potential correlations between maternal 25(OH)D status and risk of childhood overweight.

Results

Compared with sufficient 25(OH)D in pregnancy group (≥75 nmol/L), there were no correlations between the maternal 25(OH)D deficiency (<50 nmol/L) and large gestational age (LGA) (p = 0.465), small gestational age (SGA) (p = 0.607), lower birth weight (LBW) (p = 0.725) or fetal macrosomia (p = 0.535). Moreover, no significant associations between insufficient maternal 25(OH)D (50–75 nmol/L) and LGA (p = 0.505), SGA (p = 0.816), LBW (p = 0.816), or fetal macrosomia (p = 0.413) were observed. We found statistically significant disparities between the fetal birth weight and height (p < 0.001), weight (p < 0.001), head circumference (p = 0.001) of 6-years preschooler. No significant associations were found between the maternal 25(OH)D levels and BMI and risk of overweight at 6-year-old preschoolers.

Conclusion

There were no apparent correlation found between maternal 25(OH)D concentrations and fetal birth status. There was no evidence found to suggest the effect of maternal 25(OH)D level on overweight at 6-year-old preschoolers.

目的：关于母体维生素 D 状态与儿童超重和体重指数（BMI）之间的相关性，现有证据有限且相互矛盾。本研究旨在调查以下内容：(1) 母体 25- 羟基维生素 D（25(OH)D）水平与新生儿生长状况之间的潜在关联；(2) 母体 25OHD 水平与 BMI 之间的关系以及学龄前儿童超重的风险：方法：研究人员对 3213 对符合条件的单胎母婴进行了队列研究，以探讨母体 25（OH）D 水平与胎儿生长状况之间可能存在的关联。应用1767对符合条件的单胎母婴和6岁学龄前儿童的数据，分析了母亲25（OH）D水平与儿童超重风险之间的潜在相关性：结果：与孕期25（OH）D充足组（≥75 nmol/L）相比，母体25（OH）D缺乏与儿童超重风险之间没有相关性：母体 25（OH）D 浓度与胎儿出生状况之间没有明显的相关性。没有证据表明母体 25（OH）D 水平对 6 岁学龄前儿童超重有影响。

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引用次数: 0

Comparison of outcomes of labor induction with dinoprostone vaginal insert (PROPESS) and double balloon cook catheter in term nulliparous pregnancies 使用地诺前列酮阴道插入物（PROPESS）和双球囊 cook 导管对足月产孕妇进行引产的效果比较。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-06 DOI: 10.1111/jog.16107

Shiyu Li, Hua He, Wenpei Zheng, Jing Liu, Chaoli Chen

Objective

This retrospective study aimed to compare the outcomes of dinoprostone vaginal insert (PROPESS) and double balloon cook catheter (DBC) in term nulliparous pregnancies.

Materials and Methods

A total of 1682 cases were enrolled, all of which were divided into two groups: PROPESS and DBC. The primary outcomes were rate of vaginal delivery (VD) and cesarean section (CS) and successful VD within 24 h and CS indications; the secondary outcomes were labor duration, oxytocin augmentation, complications during labor, maternal/neonatal outcomes, and predictors of successful VD within 24 h.

Results

There was no statistical difference in the rates of VD between the PROPESS group (73.74%) and the DBC group (77.73%) (p = 0.36). The VD rate within 24 h was significantly higher in PROPESS group than in the DBC group (55.45% vs. 38.43%, p < 0.001). The occurrence of nonreassuring fetal heart rate (NRFHR) patterns was higher in the CS indications of the PROPESS group than in the DBC group (56.86% vs. 37.81%, p < 0.001). The oxytocin augmentation was significantly lower in the PROPESS group than in the DBC group (52.79% vs. 94.9%, p < 0.001). The incidence of chorioamnionitis was significantly higher in the DBC group as compared with PROPESS group (4.36% vs. 1.34%, p < 0.001), while neonatal outcomes were comparable between the two groups. PROPESS (p < 0.001, odds ratio [OR] 2.478, 95% confidence interval [CI] 1.718–3.574), oxytocin augmentation (p < 0.001, OR 32.759, 95% CI 20.654–51.958), and amniotomy (p = 0.016, OR 1.897, 95% CI 1.331–2.704) were predictors of VD within 24 h.

Conclusion

The effectiveness of delivery was comparable between the two groups. The PROPESS group resulted in a high successful VD rate within 24 h, although accompanied by NRFHR occurrence. Two cases with fetal presentation change (cephalic to breech) and four cases with umbilical cord prolapse were observed in the DBC group. PROPESS, oxytocin augmentation, and amniotomy were associated with VD interval within 24 h.

摘要这项回顾性研究旨在比较地诺前列酮阴道插入物（PROPESS）和双球囊妊娠导管（DBC）对足月无子宫妊娠的疗效：材料：共 1682 个病例，全部分为两组：PROPESS组和DBC组。主要结果为阴道分娩率（VD）、剖宫产率（CS）、24 小时内成功 VD 率和 CS 适应症；次要结果为产程、催产素添加量、产程并发症、产妇/新生儿结局和 24 小时内成功 VD 的预测因素：PROPESS 组（73.74%）和 DBC 组（77.73%）的顺产率没有统计学差异（P = 0.36）。PROPESS 组在 24 小时内的 VD 率明显高于 DBC 组（55.45% 对 38.43%，p 结论：PROPESS 组和 DBC 组在 24 小时内的 VD 率相当：两组的分娩效果相当。PROPESS 组在 24 小时内的顺产成功率较高，但伴随着 NRFHR 的发生。DBC 组有 2 例胎儿先露改变（头臀位）和 4 例脐带脱垂。PROPESS、催产素注射和羊膜切开术与 24 小时内的 VD 间隔有关。

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引用次数: 0

Reevaluating the variation of cesarean scar defect 重新评估剖腹产疤痕缺陷的变化。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-05 DOI: 10.1111/jog.16137

Hiroshi Kobayashi, Shogo Imanaka

Aim

The increasing incidence of cesarean sections has led to a higher prevalence of cesarean scar defects (CSDs), predominantly characterized by postmenstrual bleeding in affected women. CSD manifests in various forms, including isolated myometrial defects, intrauterine cystic protrusions, and extrauterine cystic extensions. The pathophysiological mechanisms underpinning CSD and its associated symptoms remain insufficiently understood. This review explores the pathogenesis of CSD, highlights its morphological characteristics with an emphasis on variable clinical diversity, and addresses the challenges for future research.

Methods

A comprehensive narrative review was conducted using electronic databases, including PubMed and Google Scholar, to identify 41 relevant literatures published up to December 31, 2023.

Results

The myometrium comprises two distinct layers—the inner and outer myometrium—with differences in their origin, structure, and function. Disruptions within these layers contribute to CSD development. Histopathologically, symptomatic CSD may be linked to uterine scar endometriosis, cystic adenomyosis, or endometrial cysts, suggesting that CSD, particularly those necessitating surgical intervention, are not limited to myometrial defects but may also involve iatrogenic endometriosis or adenomyosis, thereby exacerbating clinical symptoms.

Conclusion

This review provides an updated understanding of the histopathological features and classification of CSD, with an emphasis on elucidating its underlying pathogenesis.

目的：随着剖宫产率的增加，剖宫产瘢痕缺损（CSD）的发病率也越来越高，主要表现为受影响的妇女经后出血。CSD 的表现形式多种多样，包括孤立的子宫肌层缺损、宫腔内囊性突起和宫腔外囊性延伸。人们对 CSD 及其相关症状的病理生理机制仍不甚了解。这篇综述探讨了 CSD 的发病机制，强调了其形态学特征，重点是其临床多样性，并探讨了未来研究面临的挑战：方法：使用PubMed和谷歌学术等电子数据库进行了全面的叙述性综述，以确定截至2023年12月31日发表的41篇相关文献：子宫肌层由两层不同的肌层组成--子宫肌内层和子宫肌外层，它们的起源、结构和功能各不相同。这两层子宫肌层的破坏会导致 CSD 的发生。从组织病理学角度看，有症状的CSD可能与子宫瘢痕子宫内膜异位症、囊性子宫腺肌病或子宫内膜囊肿有关，这表明CSD，尤其是那些需要手术干预的CSD，并不局限于子宫肌层缺陷，还可能涉及先天性子宫内膜异位症或子宫腺肌病，从而加重临床症状：本综述提供了对 CSD 组织病理学特征和分类的最新理解，重点在于阐明其潜在的发病机制。

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引用次数: 0

Malignant peripheral nerve sheath tumor of the cervix in an adolescent with neurofibromatosis type 1: A case report and review of literature 一名患有神经纤维瘤病 1 型的青少年宫颈恶性周围神经鞘瘤：病例报告和文献综述。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-05 DOI: 10.1111/jog.16139

Nozomi Furuzono, Shinichi Togami, Ikumi Kitazono, Takuro Nishikawa, Akihide Tanimoto, Hiroaki Kobayashi

Malignant peripheral nerve sheath tumors (MPNSTs) of the cervix are rare, particularly in patients with neurofibromatosis type 1 (NF1). This report describes a cervical MPNST in an 18-year-old patient with no history of sexual activity, abnormal vaginal discharge, and prolonged menstruation. She had more than six café-au-lait spots on her body since birth and was diagnosed with NF1 at 2 years of age. Positron emission tomography-computed tomography revealed a large pelvic mass and lung and bone metastases. Biopsy confirmed MPNST. Immunohistochemical staining showed diffuse positivity for CD10, approximately 30% positivity for cyclin D1, partial positivity for α-SMA, desmin, and MyoD1, and negativity for myogenin, S-100, and SOX-10. A cancer gene panel identified several genetic abnormalities, but none were actionable mutations. Despite systemic chemotherapy, the tumor progressed rapidly, and the patient died 8 weeks post-admission. Early diagnosis of MPNST is crucial. In patients with NF1, even mild symptoms can indicate MPNST.

宫颈恶性周围神经鞘瘤（MPNST）非常罕见，尤其是在 1 型神经纤维瘤病（NF1）患者中。本报告描述了一名 18 岁患者的宫颈 MPNST，该患者无性活动史，阴道分泌物异常，月经期延长。她从出生起身上就有超过 6 个咖啡色斑块，2 岁时被诊断为 NF1。正电子发射断层计算机断层扫描显示，她的盆腔有一个大肿块，并有肺转移和骨转移。活组织检查证实了 MPNST。免疫组化染色显示 CD10 呈弥漫阳性，细胞周期蛋白 D1 阳性约 30%，α-SMA、desmin 和 MyoD1 部分阳性，肌原蛋白、S-100 和 SOX-10 阴性。癌症基因面板发现了几种基因异常，但都不是可操作的突变。尽管进行了全身化疗，但肿瘤进展迅速，患者在入院后8周死亡。MPNST 的早期诊断至关重要。在 NF1 患者中，即使是轻微的症状也可能预示着 MPNST。

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引用次数: 0

Clinical implications of genetic testing for congenital protein C deficiency in pregnancy 妊娠先天性蛋白 C 缺乏症基因检测的临床意义。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-05 DOI: 10.1111/jog.16138

Tomoka Maehana, Toshiya Nishikubo, Ryo Maekawa, Taeko Hotta, Kyohei Nishikawa, Keita Waki, Yuko Makino, Juria Akasaka, Ryuji Kawaguchi, Fuminori Kimura

Congenital protein C (PC) deficiency is a mostly autosomal dominant hereditary thrombophilia associated with early onset arterial and venous thrombotic diseases. In newborns, PC deficiency results in severe complications such as cerebral hemorrhage, cerebral infarction, and purpura fulminans, leading to death in some cases. We report two cases of deep vein thrombosis diagnosed during pregnancy that prompted genetic testing confirming definitive congenital PC deficiency. One patient with deep vein thrombosis at 30 weeks of gestation underwent anticoagulation therapy with the placement of an inferior vena cava filter. Genetic testing revealed a missense mutation in the PC gene. Another patient developed deep vein thrombosis at 9 weeks of gestation and received anticoagulant therapy, revealing a frameshift mutation in the gene. Genetic testing confirming congenital PC deficiency facilitates tailored postpartum management, including long-term anticoagulation therapy, based on the mother's thrombosis risk. For newborns, early diagnosis allows timely preparation of treatments, such as freshly thawed frozen plasma or PC replacement therapy and ensures closer monitoring through imaging evaluations, enabling early intervention to decrease the severity of potential complications. Given its utility in managing maternal and neonatal outcomes, early genetic testing in suspected cases of maternal PC deficiency is crucial before delivery.

先天性蛋白 C（PC）缺乏症主要是一种常染色体显性遗传性血栓性疾病，与早发性动脉和静脉血栓性疾病有关。在新生儿中，PC 缺乏症会导致严重的并发症，如脑出血、脑梗塞和紫癜，在某些情况下会导致死亡。我们报告了两例在怀孕期间被诊断为深静脉血栓形成的病例，这两例病例通过基因检测确诊为先天性 PC 缺乏症。其中一名患者在妊娠 30 周时出现深静脉血栓，在接受抗凝治疗的同时，植入了下腔静脉过滤器。基因检测发现该患者的 PC 基因存在错义突变。另一名患者在妊娠 9 周时出现深静脉血栓，接受了抗凝治疗，结果发现该基因存在框移突变。通过基因检测确诊先天性 PC 缺乏症后，可根据母亲的血栓形成风险，对其进行量身定制的产后管理，包括长期抗凝治疗。对于新生儿来说，早期诊断有助于及时准备治疗，如新鲜解冻的冰冻血浆或 PC 替代疗法，并确保通过影像学评估进行更密切的监测，从而进行早期干预，降低潜在并发症的严重性。鉴于 PC 缺乏症在控制孕产妇和新生儿预后方面的作用，在分娩前对疑似 PC 缺乏症孕产妇进行早期基因检测至关重要。

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引用次数: 0

Letter to “Humans-written versus ChatGPT-generated case reports” 致 "人类撰写的病例报告与 ChatGPT 生成的病例报告 "的信。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-11-04 DOI: 10.1111/jog.16127

Amnuay Kleebayooon, Viroj Wiwanitkit

引用次数: 0

Menstrual blood-derived mesenchymal stem cells combining with platelet-rich plasma infusion in endometrium repair 月经血间充质干细胞结合富血小板血浆注入修复子宫内膜。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-10-31 DOI: 10.1111/jog.16135

Yang Wang, Wenxin Liu, Hui Xu, Liyuan Dong, Yalou Jiao, Zhe Qiao

Objective

Thin endometrium caused by various factors affects the conception rate of females worldwide; however, current medications are still insufficient. Therefore, a novel approach is needed. We previously reported the effect of menstrual blood-derived mesenchymal stem cells (MenSCs) in ameliorating ethanol-induced endometrial injuries. In the present study, we aimed to investigate whether the effect of MenSCs is enhanced by a combination of platelet-rich plasma (PRP) infusion.

Methods

An endometrial injury mouse model was established by infiltrating 95% ethanol for 15 s into the uterus, followed by MenSCs, PRP, or MenSCs + PRP treatment. Pathological changes were observed by HE staining. The expression of CK18, vimentin, ItgαVβ3, and VEGF was determined using IHC staining and WB blotting.

Results

Compared with the model, MenSCs, PRP, and MenSCs + PRP treatments significantly improved endometrial damage and thickness, with the combined therapy displaying the most pronounced efficacy. The density of CK18-, vimentin-, and ItgαVβ3 positive cells increased most significantly in the MenSCs + PRP group of mice. In addition, the protein expression of CK18, vimentin, and VEGF was significantly upregulated after MenSCs + PRP, MenSCs, and PRP treatment, with MenSCs + PRP therapy showing the best efficacy.

Conclusion

MenSCs + PRP therapy is more beneficial for ameliorating ethanol-induced endometrial damage than MenSCs or PRP alone, providing a basis for the investigation of novel approaches for treating thin endometria.

目的各种因素导致的子宫内膜薄影响着全球女性的受孕率，但目前的药物治疗仍显不足。因此，需要一种新的方法。我们曾报道过经血间充质干细胞（MenSCs）在改善乙醇引起的子宫内膜损伤方面的作用。在本研究中，我们旨在探讨是否结合富血小板血浆（PRP）输注可增强间充质干细胞的作用：方法：将 95% 的乙醇浸润子宫 15 秒，然后用 MenSCs、PRP 或 MenSCs + PRP 治疗，建立子宫内膜损伤小鼠模型。通过 HE 染色观察病理变化。通过 IHC 染色和 WB 印迹检测 CK18、波形蛋白、ItgαVβ3 和 VEGF 的表达：结果：与模型相比，MenSCs、PRP 和 MenSCs + PRP 治疗能显著改善子宫内膜损伤和厚度，其中联合治疗的疗效最明显。MenSCs+PRP组小鼠CK18、波形蛋白和ItgαVβ3阳性细胞的密度增加最为明显。此外，MenSCs + PRP、MenSCs 和 PRP 治疗后，CK18、波形蛋白和血管内皮生长因子的蛋白表达明显上调，其中 MenSCs + PRP 治疗的疗效最好：结论：MenSCs + PRP疗法比单独使用MenSCs或PRP疗法更有利于改善乙醇引起的子宫内膜损伤，为研究治疗薄型子宫内膜的新方法提供了依据。

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引用次数: 0

ZC3H13-induced the m6A modification of hsa_circ_0081723 promotes cervical cancer progression via AMPK/p53 pathway ZC3H13诱导的hsa_circ_0081723的m6A修饰通过AMPK/p53途径促进宫颈癌的进展。

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research

Pub Date : 2024-10-30 DOI: 10.1111/jog.16140

Qiong Wei, Yi Yang, Chun Li, Huimin Wang

Background

N6-methyladenosine (m6A) modification and circular RNAs (circRNAs) have been confirmed to participate in cervical cancer (CC) progression. However, the function of a novel circRNA, hsa_circ_0081723, has not yet been explored in CC. Therefore, this study aimed to investigate the potential role of hsa_circ_0081723 and its m6A modification in CC.

Methods

The hsa_circ_0081723 and ZC3H13 expressions were examined by qRT-PCR in the CC tissues, and their prognostic significance was evaluated via Kaplan–Meier Plotter. The role of hsa_circ_0081723 in CC progression was checked by loss-of-function assays. The relative protein levels of AMPK/p53 pathway were determined by western blotting. The interactions of hsa_circ_0081723 and ZC3H13 were verified via MeRIP and RNA stability assays.

Results

The hsa_circ_0081723 expression was elevated in CC samples, and its higher levels indicated high histological grade, high FIGO stage, poor differentiation, and poor prognosis. Functionally, silencing hsa_circ_0081723 impaired the malignant behavior of CC cells and enhanced the protein levels of key molecules of the AMPK signaling pathway. Moreover, ZC3H13 was also elevated in CC samples and demonstrated a positive association with hsa_circ_0081723. The relative enrichment of hsa_circ_0081723 m6A and its stability were enhanced in ZC3H13 overexpressed CC cells. Mechanically, ZC3H13 overexpression partially reversed the antitumor effects caused by hsa_circ_0081723 knockdown in CC cells.

Conclusions

This study innovatively demonstrates that ZC3H13-mediated m6A modification of hsa_circ_0081723 promotes CC progression by modulating AMPK/p53 pathway. Our findings may contribute to the understanding of the molecular mechanisms underlying CC and offer potential therapeutic targets for clinical treatment.

背景：N6-甲基腺苷（m6A）修饰和环状 RNA（circRNA）已被证实参与宫颈癌（CC）的进展。然而，一种新的环状 RNA（hsa_circ_0081723）在宫颈癌中的功能尚未得到探讨。因此，本研究旨在探讨 hsa_circ_0081723 及其 m6A 修饰在 CC 中的潜在作用：方法：通过qRT-PCR检测hsa_circ_0081723和ZC3H13在CC组织中的表达，并通过Kaplan-Meier Plotter评估其预后意义。通过功能缺失实验检测了hsa_circ_0081723在CC进展中的作用。蛋白印迹法测定了AMPK/p53通路的相对蛋白水平。通过MeRIP和RNA稳定性实验验证了hsa_circ_0081723与ZC3H13的相互作用：结果：hsa_circ_0081723在CC样本中表达升高，其较高水平表明组织学分级高、FIGO分期高、分化差和预后差。从功能上讲，沉默hsa_circ_0081723可抑制CC细胞的恶性行为，并提高AMPK信号通路关键分子的蛋白水平。此外，ZC3H13也在CC样本中升高，并与hsa_circ_0081723呈正相关。在ZC3H13过表达的CC细胞中，hsa_circ_0081723 m6A的相对富集度和稳定性都有所提高。从机理上讲，ZC3H13的过表达部分逆转了敲除hsa_circ_0081723对CC细胞的抗肿瘤作用：本研究创新性地证明了 ZC3H13 介导的 hsa_circ_0081723 m6A 修饰通过调节 AMPK/p53 通路促进了 CC 的进展。我们的研究结果可能有助于理解CC的分子机制，并为临床治疗提供潜在的治疗靶点。

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