Journal of oral biology and craniofacial research最新文献_第9页

A custom-made appliance for mandibular mobilization in children with limited mouth opening 一种用于限制开口儿童下颌活动的定制器具

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-17 DOI: 10.1016/j.jobcr.2025.10.010

Mahdis Maleki, Sally Elshennawy, Paniz Haghighi, Taras Masnyi, Kyle Stevens

Introduction

Limited mouth opening can impact oral function and hygiene, necessitating surgical intervention. Adjunctive appliance therapy has been shown to improve postoperative outcomes. At The Hospital for Sick Children (SickKids), a customized Mandibular Opening Appliance (MOA) was developed to enhance jaw mobility post-surgery.

Methods

A retrospective chart review was conducted for seven patients who completed MOA therapy at our orthodontic clinic between 2021 and 2024, using data from patients' charts. Maximum incisal opening (MIO) was recorded pre-surgery, post-surgery, following MOA use, and follow-up.

Results

Pre-surgical MIO ranged from 5 to 17 mm, with a mean of 8.8 ± 5.2 mm. Among the five patients with good to excellent compliance, final MIO ranged from 18 to 42 mm, representing a mean increase of 23.2 ± 9.2 mm (range: 13–36 mm). In contrast, two patients with poor compliance showed only minimal improvement (2 mm and 4 mm).

Conclusions

These findings demonstrate the efficiency of the MOA in improving MIO in compliant pediatric patients. Incorporating such appliances post-surgery shows potential for improving mandibular mobility and supporting long-term outcomes. Although a larger sample size is needed to strengthen the evidence, current results remain compelling and support refining appliance protocols for this patient population.

口腔张开有限会影响口腔功能和卫生，需要手术干预。辅助器械治疗已被证明可以改善术后预后。在病童医院（SickKids），一种定制的下颌开口器（MOA）被开发出来，以增强术后颌骨的活动能力。方法回顾性分析2021 - 2024年在我院正畸门诊完成MOA治疗的7例患者的病历资料。记录术前、术后、MOA使用后及随访时的最大切口（MIO）。结果术前MIO范围为5 ~ 17 mm，平均8.8±5.2 mm。5例依从性良好至优异的患者，最终MIO范围为18 ~ 42 mm，平均增加23.2±9.2 mm（范围：13 ~ 36 mm）。相比之下，2例依从性较差的患者只有最小的改善（2mm和4mm）。结论MOA可有效改善依从性患儿的MIO。术后结合此类矫治器显示出改善下颌活动能力和支持长期预后的潜力。虽然需要更大的样本量来加强证据，但目前的结果仍然令人信服，并支持为这一患者群体改进器械方案。

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引用次数: 0

Efficacy of chitosan dressing as a local haemostatic agent in the management of dental extractions in patients on antiplatelet therapy. A prospective randomized study 壳聚糖敷料局部止血在拔牙患者抗血小板治疗中的疗效观察。一项前瞻性随机研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-16 DOI: 10.1016/j.jobcr.2025.10.012

Deepak Agrawal, Sabah Zaheer, Vilas Newaskar

Introduction

Dental extractions in patients on antiplatelet therapy pose a bleeding risk. Current guidelines support continuing antiplatelet therapy during surgery, but effective local hemostasis is crucial. Chitosan, a biopolymer with haemostatic, antimicrobial, and wound-healing properties, may offer advantages over cotton gauze. This study evaluated chitosan dressing vs. standard gauze during dental extractions in patients on antiplatelet therapy.

Methodology

A prospective randomized study was conducted over 18 months at the Department of Oral and Maxillofacial Surgery, Government College of Dentistry, Indore, with 100 patients on antiplatelet therapy. Extraction sites were randomly assigned to Group A (chitosan) or Group B (cotton gauze). The primary outcome was time to hemostasis, with secondary outcomes including pain (VAS), Landry healing index, postoperative bleeding, and complications. Data were analyzed using SPSS v25.0.

Results

Group A showed significantly faster hemostasis (median 0.67 min) compared to Group B (median 4.5 min; p < 0.001). Bleeding ceased within 3 min in all Group A sockets vs. 11 % in Group B (p < 0.001). Group A also had lower pain scores and higher healing index values at Day 7 (both p < 0.001). Dry socket incidence was low and similar between groups.

Conclusion

Chitosan dressing appears to be a promising adjunct for achieving rapid hemostasis, reducing postoperative discomfort, and improving early healing following dental extractions in patients on antiplatelet therapy. Larger multicenter studies with longer follow-up are recommended to confirm these findings and explore broader clinical applications.

接受抗血小板治疗的患者拔牙有出血风险。目前的指南支持手术期间继续抗血小板治疗，但有效的局部止血是至关重要的。壳聚糖是一种生物聚合物，具有止血、抗菌和伤口愈合的特性，可能比棉纱布更有优势。本研究评价了在接受抗血小板治疗的患者拔牙时，壳聚糖敷料与标准纱布的对比。方法在印度政府牙科学院口腔颌面外科对100例接受抗血小板治疗的患者进行为期18个月的前瞻性随机研究。提取部位随机分为A组（壳聚糖）和B组（棉纱）。主要终点是止血时间，次要终点包括疼痛（VAS）、Landry愈合指数、术后出血和并发症。数据采用SPSS v25.0进行分析。结果A组止血速度明显快于B组（中位4.5 min; p < 0.001），平均止血时间为0.67 min。所有A组的血槽在3分钟内停止出血，而B组为11% （p < 0.001）。A组在第7天疼痛评分较低，愈合指数值较高（p < 0.001）。干窝发生率低，组间相似。结论壳聚糖敷料对接受抗血小板治疗的拔牙患者具有快速止血、减少术后不适和促进早期愈合的作用。建议进行更大规模的多中心研究，随访时间更长，以证实这些发现，并探索更广泛的临床应用。

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引用次数: 0

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-16 DOI: 10.1016/j.jobcr.2025.10.008

Sutthida Pakdeemeechai , Jirapha Kammai , Nattakan Chaipattanawan , Napatsorn Imerb

Background

Medication-related osteonecrosis of the jaw (MRONJ) is a serious complication of antiresorptive and antiangiogenic therapies. Identifying its incidence and risk factors is vital for prevention and management.

Objective

To identify clinical and pharmacological factors associated with healing outcomes in patients with MRONJ who receiving antiresorptive or antiangiogenic therapy treated at a single center over one-year analysis period. Additionally, we examined the healing outcome and its association with MRONJ stage and anatomical location.

Methods

This single-center cohort study, combining retrospective baseline data collection with prospective longitudinal follow-up over one year in Northern Thailand. Patient demographics, medication history, clinical features, and treatment outcomes were analyzed.

Results

Seventy-four patients (79.73 % female; mean age 72.07 ± 10.78 years) with 205 sites were analyzed. The overall MRONJ prevalence was 22.44 %, highest among patients with bone metastasis (85.71 %) and multiple myeloma (23.53 %). One-year incidence reached 23.46 %, with cancer patients showing the highest incidence (100 %). Tooth extraction was the leading local risk factor (71.74 %). Overall healing rate was 67.39 %, with better outcomes in stage 1 (77.78 %) and stage 2 (68.00 %) compared to stage 3 (0 %). In stages 1 and 2, univariable analysis showed stage 1 had higher odds of healing (OR: 1.84; 95 % CI: 0.53–6.41), and multivariable analysis confirmed this trend (adjusted OR: 3.51; 95 % CI: 0.16–76.4), though neither was statistically significant.

Conclusion

MRONJ prevalence and incidence remain substantial in high-risk populations. Better healing was observed in early-stage lesions, emphasizing the importance of early detection and timely intervention to improve clinical outcomes.

背景：药物相关性颌骨骨坏死（MRONJ）是抗吸收和抗血管生成治疗的严重并发症。确定其发病率和危险因素对预防和管理至关重要。目的在为期一年的分析期内，确定在单一中心接受抗吸收或抗血管生成治疗的MRONJ患者与愈合结果相关的临床和药理学因素。此外，我们研究了愈合结果及其与MRONJ分期和解剖位置的关系。方法本研究采用单中心队列研究，在泰国北部进行回顾性基线数据收集和前瞻性纵向随访，随访时间超过一年。分析患者人口统计学、用药史、临床特征和治疗结果。结果共分析205个部位74例，女性79.73%，平均年龄72.07±10.78岁。MRONJ的总患病率为22.44%，以骨转移（85.71%）和多发性骨髓瘤（23.53%）患者最高。1年发病率为23.46%，肿瘤患者发病率最高（100%）。拔牙是主要的局部危险因素（71.74%）。总治愈率为67.39%，1期（77.78%）和2期（68.00%）优于3期（0%）。在第一阶段和第二阶段，单变量分析显示第一阶段有更高的愈合几率（OR: 1.84; 95% CI: 0.53-6.41），多变量分析证实了这一趋势（调整OR: 3.51; 95% CI: 0.16-76.4），尽管两者均无统计学意义。结论mronj在高危人群中的患病率和发病率仍然较高。在早期病变中观察到较好的愈合，强调早期发现和及时干预对改善临床结果的重要性。

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引用次数: 0

Assessment of the success and survival of full mouth rehabilitations: a 3 year follow up study 评估全口康复的成功和生存：一项为期3年的随访研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-15 DOI: 10.1016/j.jobcr.2025.10.007

Divyansh Sinha, Suresh Venugopalan, Vijay Anand

Introduction

Full mouth rehabilitation (FMR) addresses complex dental issues like tooth wear, cosmetic concerns, and loss of occlusal stability, enhancing both function and aesthetics. This study aimed to evaluate the success and survival of tooth-supported FMR treatments and identify common causes of treatment failure.

Methodology

A retrospective analysis of 60 patients who underwent FMR between 2019 and 2021 was conducted. Data were collected from the Dental Information Archiving Software (DIAS) and analyzed using modified USPHS criteria, covering parameters like postoperative sensitivity, anatomical form, and marginal adaptation, marginal discoloration, surface texture and recurrent caries. Additional aesthetic and functional discrepancies were also documented.

Results

At cementation, all parameters scored 100 % alpha. At 3-year follow-up, slight declines were observed in sensitivity (99.17 % alpha), anatomic form (99.17 % alpha), marginal discoloration (98.88 % alpha), and recurrent caries (99.17 % alpha), with no statistical significance. Marginal adaptation (97.22 % alpha, p = 0.004) and surface texture (97.5 % alpha, p = 0.006) showed significant differences. Aesthetic issues included midline disparities (34 %) and ceramic chipping (30 %), while functional issues included absence of incisal guidance (23 %) and occlusal plane discrepancies (16 %).

Discussion

Findings suggest that successful FMR relies on a multidisciplinary approach and advanced CAD/CAM technology, which enhances accuracy and reduces treatment time. However, common aesthetic issues, such as gingival zenith variations, highlight areas for further improvement.

全口康复（FMR）解决复杂的牙齿问题，如牙齿磨损、美容问题和咬合稳定性丧失，增强功能和美观。本研究旨在评估牙支持FMR治疗的成功和生存，并确定治疗失败的常见原因。方法回顾性分析2019 - 2021年间60例FMR患者。从牙科信息存档软件（DIAS）中收集数据，并使用改进的USPHS标准进行分析，包括术后敏感性、解剖形态、边缘适应、边缘变色、表面纹理和复发性龋病等参数。还记录了其他美学和功能差异。结果胶结时，各项指标α评分均为100%。3年随访，敏感性（99.17% α）、解剖形态（99.17% α）、边缘变色（98.88% α）、龋齿复发（99.17% α）略有下降，差异无统计学意义。边缘适应性（97.22% α, p = 0.004）和表面纹理（97.5% α, p = 0.006）差异有统计学意义。美学问题包括中线差异（34%）和陶瓷碎片（30%），而功能问题包括缺切引导（23%）和咬合平面差异（16%）。研究结果表明，成功的FMR依赖于多学科方法和先进的CAD/CAM技术，这可以提高准确性并缩短治疗时间。然而，常见的美学问题，如牙龈天顶变化，突出了需要进一步改善的领域。

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引用次数: 0

A novel approach utilizing Β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin- loaded chitosan microspheres for periodontal regeneration in two-wall intra bony defects 利用Β-tricalcium磷酸盐/明胶复合支架结合庆大霉素负载壳聚糖微球修复两壁骨内缺损的新方法

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-15 DOI: 10.1016/j.jobcr.2025.10.004

Mohamed Hamdy Helal , Aya Anwar Alsherif , Yasmin Hamdy , Malak Yousef Mohamed Shoukheba , Khaled M. Ali , Mohamed Yehia Abdelfattah , Moustafa Nabil Aboushelib

Objectives

There is an increasing demand for innovative biomaterials, scaffolds and techniques in dental practices to improve regenerative therapies. Researchers studied periodontium regeneration using biologically compatible scaffolds to repair periodontal and bony defects. The present study aimed to evaluate the effect of β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for periodontal regeneration in two-wall intra bony defects in Dogs.

Design

12 bilateral intrabony defects (total 24 defects), 5 mm height, and 3 mm depth, were surgically created on the mesial surface of second mandibular premolars and allowed to become chronically healed in 12 mongrel male dogs. Dogs were randomly divided into three groups: Group I (8 defects) received β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres. Group II (8 defects) received the same scaffolds without gentamycin. Group III (8 defects) received open flap debridement (OFD) as a control. The animals were sacrificed at 8 weeks post-operatively for histological and histomorphometry analysis (n = 8, α = 0.05).

Results

In comparison to the control, both test groups exhibited the highest percentage of newly formed bone height, newly formed bone area, newly formed cementum, and newly formed periodontal tissues, indicating a substantial (P < 0.001) increase in new regenerative tissue. However, in the control group, epithelial downgrowth predominated.

Conclusions

Within the limitations of this study, the novel treatment approach using β-TCP/Gelatin Scaffolds with Gentamicin-Loaded Chitosan improved periodontal regeneration and wound healing in two-wall intra bony defects in Dogs.

目的在牙科实践中，对创新生物材料、支架和技术的需求不断增加，以改善再生治疗。研究人员研究了使用生物相容性支架修复牙周和骨缺损的牙周组织再生。本研究旨在评价β-磷酸三钙/明胶复合支架结合庆大霉素壳聚糖微球修复犬双壁骨缺损的效果。设计12只雄性杂种犬，在第二下颌前磨牙的内侧表面手术形成12个双侧骨内缺损（共24个缺损），高度为5mm，深度为3mm，并允许其长期愈合。将犬随机分为3组：1组（8个缺陷）采用β-磷酸三钙/明胶复合支架，外加负载庆大霉素的壳聚糖微球；II组（8个缺陷）采用相同的支架，不使用庆大霉素。第三组（8个缺损）采用开放式皮瓣清创（OFD）作为对照。术后8周处死动物进行组织学和组织形态学分析（n = 8, α = 0.05）。结果与对照组相比，两组新形成的骨高度、新形成的骨面积、新形成的牙骨质和新形成的牙周组织的百分比最高，表明新再生组织的显著增加（P < 0.001）。然而，在对照组中，上皮细胞的下降占主导地位。结论在本研究的局限性内，β-TCP/明胶支架与庆大霉素负载壳聚糖的新型治疗方法可改善犬双壁骨内缺损的牙周再生和伤口愈合。

{"title":"A novel approach utilizing Β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin- loaded chitosan microspheres for periodontal regeneration in two-wall intra bony defects","authors":"Mohamed Hamdy Helal , Aya Anwar Alsherif , Yasmin Hamdy , Malak Yousef Mohamed Shoukheba , Khaled M. Ali , Mohamed Yehia Abdelfattah , Moustafa Nabil Aboushelib","doi":"10.1016/j.jobcr.2025.10.004","DOIUrl":"10.1016/j.jobcr.2025.10.004","url":null,"abstract":"<div><h3>Objectives</h3><div>There is an increasing demand for innovative biomaterials, scaffolds and techniques in dental practices to improve regenerative therapies. Researchers studied periodontium regeneration using biologically compatible scaffolds to repair periodontal and bony defects. The present study aimed to evaluate the effect of β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for periodontal regeneration in two-wall intra bony defects in Dogs.</div></div><div><h3>Design</h3><div>12 bilateral intrabony defects (total 24 defects), 5 mm height, and 3 mm depth, were surgically created on the mesial surface of second mandibular premolars and allowed to become chronically healed in 12 mongrel male dogs. Dogs were randomly divided into three groups: Group I (8 defects) received β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres. Group II (8 defects) received the same scaffolds without gentamycin. Group III (8 defects) received open flap debridement (OFD) as a control. The animals were sacrificed at 8 weeks post-operatively for histological and histomorphometry analysis (n = 8, α = 0.05).</div></div><div><h3>Results</h3><div>In comparison to the control, both test groups exhibited the highest percentage of newly formed bone height, newly formed bone area, newly formed cementum, and newly formed periodontal tissues, indicating a substantial (P < 0.001) increase in new regenerative tissue. However, in the control group, epithelial downgrowth predominated.</div></div><div><h3>Conclusions</h3><div>Within the limitations of this study, the novel treatment approach using β-TCP/Gelatin Scaffolds with Gentamicin-Loaded Chitosan improved periodontal regeneration and wound healing in two-wall intra bony defects in Dogs.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1696-1709"},"PeriodicalIF":0.0,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145325161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphotype stratification of radix entomolaris in mandibular molars 下颌骨磨牙昆虫根的形态型分层

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-14 DOI: 10.1016/j.jobcr.2025.10.001

Siva Shankar Dev , Ramya Ramadoss , K. Nitya , Sandhya Sundar , Suganya Panneer Selvam , K. Hema Shree

Background

Radix entomolaris (RE), a distolingual supernumerary root in mandibular first molars, presents significant challenges in endodontic treatment due to its complex and variable anatomy. Conventional radiographic techniques often fail to detect such intricacies, increasing the risk of missed canals and treatment failure.

Objective

This study aimed to characterize the morphometric complexity of RE using cone-beam computed tomography (CBCT) and to develop machine learning (ML) models for classifying root canal morphotypes and predicting anatomical bifurcation.

Methods

One hundred extracted mandibular first molars with confirmed RE were scanned using high-resolution CBCT. Morphometric parameters—including canal curvature, cross-sectional area, roundness index (a circularity metric), volumetric size, and root fusion status (fused vs. separate roots)—were extracted at multiple apical levels. These features were used to train supervised and unsupervised ML models. A decision tree classifier predicted bifurcation presence using four anatomical features, while K-means clustering (k = 2) stratified morphotypes.

Results

The decision tree classifier achieved an F1-score of 0.87, with a sensitivity of 85.7 % and specificity of 89.4 %. Volumetric canal size was the strongest predictor of bifurcation (AUC = 0.81). K-means clustering identified two morphotypes: simple (round, single orifice) and complex (irregular, bifurcated, or C-shaped canals). Notably, roundness index decreased coronally, and 27 % of samples exhibited mid-root bifurcation.

Conclusion

CBCT-derived features, particularly canal volume and curvature, effectively predict RE complexity. The integration of these features into machine learning models provides a clinically valuable framework for morphotype classification and personalized endodontic planning. These findings support the adoption of AI-assisted diagnostics in managing anatomically complex root canal systems.

昆虫根（RE）是下颌第一磨牙的双舌多根，由于其复杂多变的解剖结构，在牙髓治疗中面临着巨大的挑战。传统的放射技术往往不能检测到这种复杂性，增加了漏管和治疗失败的风险。目的利用锥形束计算机断层扫描（CBCT）表征根管形态复杂性，并建立机器学习（ML）模型用于根管形态分类和预测根管解剖分叉。方法采用高分辨率CBCT对100颗经确诊的下颌第一磨牙进行扫描。形态测量参数——包括根管曲率、横截面积、圆度指数（一种圆度指标）、体积大小和根融合状态（融合根与分离根）——在多个根尖水平上提取。这些特征被用来训练有监督和无监督的ML模型。决策树分类器使用四种解剖特征预测分叉的存在，而k -means聚类（k = 2）分层形态。结果决策树分类器的f1评分为0.87，灵敏度为85.7%，特异性为89.4%。容量管大小是分叉最强的预测因子（AUC = 0.81）。K-means聚类鉴定出两种形态：简单（圆形、单孔）和复杂（不规则、分叉或c形管）。值得注意的是，圆度指数呈冠状下降，27%的样品出现中根分叉。结论cbct衍生的特征，尤其是椎管体积和曲率，可有效预测RE复杂性。将这些特征整合到机器学习模型中，为形态分类和个性化牙髓规划提供了一个有临床价值的框架。这些发现支持采用人工智能辅助诊断来管理解剖复杂的根管系统。

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引用次数: 0

Quantitative evaluation of spatial heterogeneity in dentin attrition using combined spectroscopic and elemental profiling 结合光谱和元素分析定量评价牙本质磨损的空间异质性

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-14 DOI: 10.1016/j.jobcr.2025.09.026

Sibi Charan, Ramya Ramadoss, Nitya Krishnasamy, Sandhya Sundar, Suganya Panneer Selvam, K. Hema Shree

Background

Age-related attrition in dentin is associated with gradual mineral loss and collagen degradation, affecting its structural and functional integrity. However, there remains a need for standardized, quantitative indices to assess these degenerative changes.

Objective

This study aimed to evaluate the structural, chemical, and elemental changes in healthy versus attrited dentin using a multimodal analytical approach and to introduce novel quantitative indices for assessing dentin degradation.

Methods

Human teeth exhibiting signs of attrition were collected, processed, and analyzed using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), inductively coupled plasma mass spectrometry (ICP-MS). Four novel indices—Crystallinity Loss Index (CLI), Mineral Integrity Index (MII), Collagen Degradation Ratio (CDR), and Structural Degradation Index (SDI)—were developed and applied.

Results

Attrited dentin showed significantly reduced crystallinity (CLI = 25.2 %), decreased mineral integrity (MII = 0.38), and marked collagen loss (CDR = 0.60) compared to healthy samples. Elemental analysis revealed a decreased Ca/P ratio and lower concentrations of Zn and Sr, with elevated Fe and Cu levels in aged dentin. The SDI (0.237) provided a composite representation of overall dentin degradation.

Conclusion

The combined use of spectroscopic and elemental analyses, along with the application of novel quantitative indices, enables a robust evaluation of dentin degradation associated with attrition. These metrics hold potential in clinical diagnosis, forensic dentistry, and restorative treatment planning.

年龄相关的牙本质磨损与逐渐丢失的矿物质和胶原蛋白降解有关，影响其结构和功能的完整性。然而，仍然需要标准化的定量指标来评估这些退行性变化。目的采用多模态分析方法评价健康牙本质与受损牙本质的结构、化学和元素变化，并引入新的定量指标来评估牙本质降解。方法采用x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、电感耦合等离子体质谱（ICP-MS）等方法对有磨损痕迹的人牙齿进行处理和分析。提出并应用了结晶度损失指数（CLI）、矿物完整性指数（MII）、胶原蛋白降解率（CDR）和结构降解指数（SDI）四个新指标。结果与健康牙本质相比，牙本质的结晶度明显降低（CLI = 25.2%），矿物质完整性降低（MII = 0.38），胶原蛋白损失明显（CDR = 0.60）。元素分析显示老年牙本质Ca/P比值降低，Zn和Sr浓度降低，Fe和Cu含量升高。SDI（0.237）提供了总体牙本质降解的综合表征。结论光谱分析和元素分析的结合，以及新的定量指标的应用，能够对与磨耗相关的牙本质降解进行可靠的评估。这些指标在临床诊断、法医牙科和恢复性治疗计划中具有潜力。

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引用次数: 0

Assessment of selective salivary properties and taste perception in subjects with oral submucous fibrosis – A case-control study 评估口腔黏膜下纤维化患者的选择性唾液特性和味觉感知-一项病例对照研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-13 DOI: 10.1016/j.jobcr.2025.09.017

Mauli Alkesh Mody , Pritesh B. Ruparelia , Manali Patel , Kosha P. Ruparelia

Background

The present study was conducted to evaluate and compare selective salivary properties and taste perception in subjects with and without oral submucous fibrosis (OSMF), since OSMF affects the properties of saliva and may lead to decline in the quality of life.

Materials and method

Eighty subjects were enrolled in the study and equally divided into two groups based on the presence or absence of OSMF, for estimating, analysing and comparing the salivary flow rate (SFR), pH and taste perception using Modified Schirmer strip, pH strip and taste solutions respectively, in both groups. Chi-square test and unpaired t-test were applied.

Results

Compared to the control group, a statistically significant decrease in SFR was noted among stage 4 OSMF subjects. Alkaline pH was present in OSMF group, regardless of the stage. Taste alterations were present in relation to sweet, salty and sour taste in subjects with OSMF.

Conclusion

There is a marked decrease in SFR, alteration in salivary pH and significant taste changes such as hypogeusia, dysgeusia and ageusia in sweet, salt and sour tastes in subjects with OSMF.

本研究旨在评估和比较有和没有口腔粘膜下纤维化（OSMF）的受试者的选择性唾液特性和味觉感知，因为OSMF会影响唾液的特性，并可能导致生活质量下降。材料与方法将80名受试者纳入研究，根据是否存在OSMF将其平均分为两组，分别使用改良Schirmer试纸、pH试纸和味觉溶液对两组的唾液流率（SFR）、pH值和味觉感知进行估计、分析和比较。采用卡方检验和非配对t检验。结果与对照组相比，4期OSMF患者的SFR有统计学意义的显著下降。不论分期，OSMF组均呈碱性。味觉改变与OSMF受试者的甜味、咸味和酸味有关。结论OSMF患者的SFR明显下降，唾液pH值明显改变，甜味、咸味、酸味出现味觉减退、味觉障碍和衰老等味觉变化。

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引用次数: 0

Thermomechanical DNA extraction from infected dental pulp for next-generation sequencing applications 从感染牙髓中热机械提取DNA用于下一代测序应用

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-10 DOI: 10.1016/j.jobcr.2025.09.029

Preethesh Shetty, Raksha Bhat, Shishir Shetty

Background

DNA extraction from infected dental pulp tissue represents a critical methodological limitation in molecular endodontics, severely constraining pathogen identification and precision therapeutic approaches. Conventional extraction protocols demonstrate systematic failures when applied to inflamed pulp samples containing complex hydroxyapatite-collagen matrices, neutrophil extracellular traps, and inflammatory mediators that compromise nucleic acid integrity and downstream next-generation sequencing applications.

Methods

The present investigation comprehensively validated a thermomechanical extraction protocol combining optimized extended thermal incubation with intensive mechanical disruption cycles specifically designed for infected dental pulp tissues. Performance was systematically evaluated against the current standardised systems using multi-parameter quality assessment, statistical analysis including effect size calculations across 24 infected pulp samples from patients with irreversible pulpitis.

Results

The thermomechanical protocol achieved transformative improvements across all performance metrics: 3.7-fold enhancement in DNA concentration (69.8 ± 10.21 vs. 18.83 ± 12.72 ng/μL, p < 0.01, Cohen's d = 4.2), 18 % improvement in protein purity ratios (A₂₆₀/A₂₈₀: 2.23 ± 0.23 vs. 1.89 ± 0.060, p < 0.01), and unprecedented 4–6 fold enhancement in inter-sample reproducibility (coefficient of variation reduction from 67.6 % to 14.6 %). Universal optimal quality classification was achieved (100 % vs. 58.3 % for conventional protocols), enabling reliable endodontic microbiome analysis and pulp genomics applications.

Conclusions

The current thermomechanical approach establishes performance standards for infected dental pulp DNA extraction, providing reliable methodology for endodontic microbiome analysis, pulp-dentin genomics, and precision therapy selection. The superior reproducibility and pathophysiology-specific refinement positions it as essential for advancing molecular endodontics and evidence-based therapeutic decision-making.

从感染牙髓组织中提取dna是分子牙髓学中一个关键的方法学限制，严重限制了病原体鉴定和精确治疗方法。传统的提取方法在处理含有复杂羟基磷灰石-胶原基质、中性粒细胞胞外陷阱和炎症介质的炎症牙髓样品时显示出系统性的失败，这些物质会损害核酸的完整性和下游下一代测序的应用。方法本研究综合验证了针对感染牙髓组织设计的热机械提取方案，该方案结合了优化的延长热培养和强化的机械破坏循环。采用多参数质量评估和统计分析，包括对来自不可逆牙髓炎患者的24个感染牙髓样本的效应量计算，对当前标准化系统的性能进行系统评估。结果热机械方案在所有性能指标上都取得了革命性的改善：DNA浓度提高3.7倍（69.8±10.21 vs. 18.83±12.72 ng/μL, p < 0.01, Cohen’s d = 4.2），蛋白质纯度比提高18% (A260/A280: 2.23±0.23 vs. 1.89±0.060,p < 0.01)，样品间可重复性提高4-6倍（变异系数从67.6%降低到14.6%）。实现了普遍的最佳质量分类（100%，而传统方案为58.3%），实现了可靠的牙髓微生物组分析和牙髓基因组学应用。结论热力学方法建立了感染牙髓DNA提取的性能标准，为牙髓微生物组分析、牙髓-牙本质基因组学和精确治疗选择提供了可靠的方法。优越的可重复性和病理生理特异性的细化定位它是必不可少的推进分子牙髓学和循证治疗决策。

{"title":"Thermomechanical DNA extraction from infected dental pulp for next-generation sequencing applications","authors":"Preethesh Shetty, Raksha Bhat, Shishir Shetty","doi":"10.1016/j.jobcr.2025.09.029","DOIUrl":"10.1016/j.jobcr.2025.09.029","url":null,"abstract":"<div><h3>Background</h3><div>DNA extraction from infected dental pulp tissue represents a critical methodological limitation in molecular endodontics, severely constraining pathogen identification and precision therapeutic approaches. Conventional extraction protocols demonstrate systematic failures when applied to inflamed pulp samples containing complex hydroxyapatite-collagen matrices, neutrophil extracellular traps, and inflammatory mediators that compromise nucleic acid integrity and downstream next-generation sequencing applications.</div></div><div><h3>Methods</h3><div>The present investigation comprehensively validated a thermomechanical extraction protocol combining optimized extended thermal incubation with intensive mechanical disruption cycles specifically designed for infected dental pulp tissues. Performance was systematically evaluated against the current standardised systems using multi-parameter quality assessment, statistical analysis including effect size calculations across 24 infected pulp samples from patients with irreversible pulpitis.</div></div><div><h3>Results</h3><div>The thermomechanical protocol achieved transformative improvements across all performance metrics: 3.7-fold enhancement in DNA concentration (69.8 ± 10.21 vs. 18.83 ± 12.72 ng/μL, p < 0.01, Cohen's d = 4.2), 18 % improvement in protein purity ratios (A<sub>260</sub>/A<sub>280</sub>: 2.23 ± 0.23 vs. 1.89 ± 0.060, p < 0.01), and unprecedented 4–6 fold enhancement in inter-sample reproducibility (coefficient of variation reduction from 67.6 % to 14.6 %). Universal optimal quality classification was achieved (100 % vs. 58.3 % for conventional protocols), enabling reliable endodontic microbiome analysis and pulp genomics applications.</div></div><div><h3>Conclusions</h3><div>The current thermomechanical approach establishes performance standards for infected dental pulp DNA extraction, providing reliable methodology for endodontic microbiome analysis, pulp-dentin genomics, and precision therapy selection. The superior reproducibility and pathophysiology-specific refinement positions it as essential for advancing molecular endodontics and evidence-based therapeutic decision-making.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1668-1675"},"PeriodicalIF":0.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of Hyaluronic acid and injectable platelet-rich fibrin as a non-surgical approach for management of black triangle 透明质酸与注射富血小板纤维蛋白非手术治疗黑三角的比较评价

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-10-08 DOI: 10.1016/j.jobcr.2025.09.030

Mansi Pal, Nand Lal, Umesh Pratap Verma, Anjani Kumar Pathak

Aims

To compare and evaluate the clinical efficacy of Hyaluronic Acid and Injectable Platelet Rich Fibrin (i-PRF) in the management of gingival black triangles using clinical and photographic analysis over 6 months.

Material and method

Thirty patients (15 in each group) were evaluated in this comparative study. Patients were divided into two groups: Group(HA) and Group(i-PRF). Injections were administered at baseline, 3 weeks, and 6 weeks. Black triangle height and area were measured at baseline, 1 month, 3 months, and 6 months using a UNC-15 probe and ImageJ software. Data were analyzed using repeated measures ANOVA and Tukey's post hoc test.

Results

Both the HA and i-PRF groups showed significant reductions in black triangle height and area over a 6-month period. HA reduced height by 0.80 mm at 1 month (p = 0.067), 1.47 mm at 3 months (p < 0.001), and 2.00 mm at 6 months (p < 0.001), while i-PRF reduced height by 1.20 mm (p = 0.003), 1.80 mm (p < 0.001), and 2.27 mm (p < 0.001) at the same intervals. Area reductions followed similar patterns. No significant intergroup differences were observed.

Conclusion

HA and i-PRF are effective, minimally invasive, and biocompatible treatments for managing black triangles. HA offers ease of use and patient comfort, while i-PRF is biologically driven and cost-effective. Material selection should be based on clinical conditions and patient preferences.

目的比较和评价透明质酸与富血小板纤维蛋白（i-PRF）治疗6个月牙龈黑三角的临床疗效。材料与方法30例患者（每组15例）进行比较研究。患者分为两组：HA组和i-PRF组。在基线、3周和6周进行注射。使用UNC-15探针和ImageJ软件在基线、1个月、3个月和6个月测量黑色三角形的高度和面积。数据分析采用重复测量方差分析和Tukey事后检验。结果在6个月的时间里，HA组和i-PRF组的黑三角高度和面积均有显著降低。HA在1个月降低高度0.80 mm (p = 0.067)，在3个月降低高度1.47 mm (p < 0.001)，在6个月降低高度2.00 mm (p < 0.001)，而i-PRF在相同的间隔降低高度1.20 mm (p = 0.003), 1.80 mm （p < 0.001）和2.27 mm （p < 0.001）。面积的减少也有类似的模式。各组间无显著差异。结论ha和i-PRF治疗黑三角是一种有效、微创、生物相容性好的治疗方法。HA提供了易用性和患者舒适度，而i-PRF是生物驱动的，具有成本效益。材料的选择应根据临床情况和患者的偏好。

{"title":"Comparative evaluation of Hyaluronic acid and injectable platelet-rich fibrin as a non-surgical approach for management of black triangle","authors":"Mansi Pal, Nand Lal, Umesh Pratap Verma, Anjani Kumar Pathak","doi":"10.1016/j.jobcr.2025.09.030","DOIUrl":"10.1016/j.jobcr.2025.09.030","url":null,"abstract":"<div><h3>Aims</h3><div>To compare and evaluate the clinical efficacy of Hyaluronic Acid and Injectable Platelet Rich Fibrin (i-PRF) in the management of gingival black triangles using clinical and photographic analysis over 6 months.</div></div><div><h3>Material and method</h3><div>Thirty patients (15 in each group) were evaluated in this comparative study. Patients were divided into two groups: <u>Group(HA) and Group(i-PRF).</u> Injections were administered at baseline, 3 weeks, and 6 weeks. Black triangle height and area were measured at baseline, 1 month, 3 months, and 6 months using a UNC-15 probe and ImageJ software. Data were analyzed using repeated measures ANOVA and Tukey's post hoc test.</div></div><div><h3>Results</h3><div>Both the HA and i-PRF groups showed significant reductions in black triangle height and area over a 6-month period. HA reduced height by 0.80 mm at 1 month (p = 0.067), 1.47 mm at 3 months (p < 0.001), and 2.00 mm at 6 months (p < 0.001), while i-PRF reduced height by 1.20 mm (p = 0.003), 1.80 mm (p < 0.001), and 2.27 mm (p < 0.001) at the same intervals. Area reductions followed similar patterns. No significant intergroup differences were observed.</div></div><div><h3>Conclusion</h3><div>HA and i-PRF are effective, minimally invasive, and biocompatible treatments for managing black triangles. HA offers ease of use and patient comfort, while i-PRF is biologically driven and cost-effective. Material selection should be based on clinical conditions and patient preferences.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1660-1667"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0