Journal of oral biology and craniofacial research最新文献_第7页

Comparative analysis of clinical, patient-centered, and COL1A1 gene expression outcomes following coronally advanced flap with xenogeneic collagen matrix versus connective tissue graft in maxillary gingival recession: A parallel-arm, single-blinded randomized controlled clinical trial 异种胶原基质冠状进展皮瓣与结缔组织移植物在上颌龈退缩中的临床、以患者为中心和COL1A1基因表达结果的比较分析：一项平行组、单盲随机对照临床试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-12-17 DOI: 10.1016/j.jobcr.2025.12.010

Vazeeha Afrin Syed, Arvina Rajasekar

Objectives

Coronally advanced flap (CAF) combined with connective tissue graft (CTG) is a gold standard for root coverage but is limited by donor site morbidity. The aim of this study was to compare the clinical, patient-centered, and molecular outcomes of CAF combined with xenogeneic collagen matrix (XCM) versus CTG in the treatment of maxillary gingival recession defects, with a focus on COL1A1 gene expression.

Methods

This randomized controlled trial included 40 patients with Cairo RT1 gingival recession defects, allocated into two groups: CAF + XCM (test; n = 20) and CAF + CTG (control; n = 20). Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL), width of keratinized tissue (WKT), recession height (RH), recession width (RW), and mean root coverage (MRC) were evaluated at baseline and 6 months. Root sensitivity was assessed at baseline and 6 months, while postoperative pain was recorded at 24 h, 7 days, and 14 days. Surgical duration was measured, and COL1A1 gene expression in gingival crevicular fluid was quantified using real-time quantitative PCR.

Results

Both groups showed significant clinical improvement and upregulation of COL1A1 expression at 6 months (p < 0.05). Intergroup differences in clinical and molecular outcomes were not statistically significant. However, the XCM group had significantly shorter surgical time and lower postoperative pain scores (p < 0.05), indicating improved patient comfort.

Conclusions

XCM offers comparable clinical and molecular outcomes to CTG with the added benefits of reduced surgical time and morbidity, making it a viable, patient-friendly alternative in the management of gingival recession.

目的冠状进展皮瓣（CAF）联合结缔组织移植物（CTG）是根覆盖的金标准，但受供区发病率的限制。本研究的目的是比较CAF联合异种胶原基质（XCM）与CTG治疗上颌龈退缩缺陷的临床、患者和分子结果，重点关注COL1A1基因表达。方法随机对照试验纳入40例Cairo RT1龈退缩缺损患者，分为CAF + XCM组（试验组，n = 20）和CAF + CTG组（对照组，n = 20）。临床参数包括探测袋深度（PPD）、临床附着水平（CAL）、角化组织宽度（WKT）、退退高度（RH）、退退宽度（RW）和平均根覆盖（MRC）在基线和6个月进行评估。在基线和6个月时评估牙根敏感性，在术后24小时、7天和14天记录疼痛。测量手术时间，采用实时定量PCR法定量龈沟液COL1A1基因表达。结果两组患者临床表现均有明显改善，6个月时COL1A1表达水平均有明显上调（p < 0.05）。组间临床和分子预后差异无统计学意义。而XCM组手术时间明显缩短，术后疼痛评分明显降低（p < 0.05），患者舒适度明显提高。结论：与CTG相比，sxcm具有相当的临床和分子结果，并具有减少手术时间和发病率的优点，使其成为治疗牙龈萎缩的可行的、患者友好的选择。

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引用次数: 0

Evaluation of bioactive extracellular soluble Hemarina (M101) derived scaffolds for neovascularization in periodontal tissue regeneration 生物活性细胞外可溶性海玛丽娜（M101）衍生支架在牙周组织再生中用于新生血管的评价

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2026-01-13 DOI: 10.1016/j.jobcr.2026.01.003

A. Mohamed Thaha, Kaarthikeyan Gurumoorthy

Background

Periodontitis leads to progressive destruction of periodontal tissues, where low oxygen levels and inadequate vascularization limit regenerative healing. Hemarina-101 (M101), a marine-sourced extracellular hemoglobin with high oxygen-binding and antioxidant potential, may overcome these barriers. This study investigated the ability of M101-incorporated chitosan–alginate scaffolds to support angiogenesis and cell compatibility for potential use in periodontal regeneration.

Methods

Chitosan–alginate scaffolds containing 10 % w/w M101 were fabricated and compared with plain scaffolds, untreated controls, and vascular endothelial growth factor (VEGF)-treated groups. Human periodontal ligament cells (PDLCs) and endothelial cells (EA.hy926) were cultured on the scaffolds. Cell viability and proliferation were analyzed using MTT assays and live/dead staining. Expression of angiogenic genes (VEGF-A, ANGPT1, CD31, HIF-1α) was quantified by qPCR. In vivo-like angiogenic responses were assessed using the chorioallantoic membrane (CAM) assay. Statistical significance was set at p < 0.05.

Results

Scaffolds with M101 showed significantly greater cell viability and proliferation than plain scaffolds (p < 0.05), comparable to VEGF-treated controls. Live/dead staining confirmed high densities of viable cells on M101 scaffolds. Gene expression analysis revealed notable upregulation of VEGF-A, ANGPT1, CD31, and HIF-1α in the M101 group, approaching levels seen with VEGF treatment. The CAM assay demonstrated dense, radially organized vessel networks forming around M101 scaffolds, indicating a strong pro-angiogenic effect.

Conclusion

M101-incorporated scaffolds enhanced endothelial cell growth, angiogenic gene activation, and neovascularization compared with plain scaffolds. These findings support the potential of M101-based biomaterials as promising candidates for periodontal tissue regeneration, meriting further preclinical and clinical validation.

牙周炎导致牙周组织的进行性破坏，其中低氧水平和血管化不足限制了再生愈合。Hemarina-101 （M101）是一种海洋来源的细胞外血红蛋白，具有高氧结合和抗氧化潜力，可以克服这些障碍。本研究探讨了m101掺入壳聚糖-海藻酸盐支架支持血管生成和细胞相容性的能力，为牙周再生提供潜在的应用前景。方法制备含有10% w/w M101的海藻酸壳聚糖支架，并与普通支架组、未处理组和血管内皮生长因子（VEGF）处理组进行比较。在支架上培养人牙周韧带细胞（pdlc）和内皮细胞（EA.hy926）。采用MTT法和活/死染色分析细胞活力和增殖。qPCR检测血管生成基因VEGF-A、ANGPT1、CD31、HIF-1α的表达。使用绒毛膜尿囊膜（CAM）试验评估体内样血管生成反应。p <； 0.05为统计学意义。结果M101支架的细胞活力和增殖能力明显高于普通支架（p < 0.05），与vegf处理的对照组相当。活/死染色证实M101支架上存在高密度活细胞。基因表达分析显示，M101组VEGF- a、ANGPT1、CD31和HIF-1α显著上调，接近VEGF治疗组的水平。CAM实验显示密集的、放射状组织的血管网络在M101支架周围形成，表明有很强的促血管生成作用。结论与普通支架相比，m101掺入支架可促进内皮细胞生长、血管生成基因激活和新生血管形成。这些发现支持了基于m101的生物材料作为牙周组织再生的有希望的候选材料的潜力，值得进一步的临床前和临床验证。

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引用次数: 0

Acknowledgement to Reviewers 审稿人致谢

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2026-02-18 DOI: 10.1016/S2212-4268(26)00020-5

引用次数: 0

Diagnostic efficiency of digital photography and AI-assisted image interpretation in dental caries examination: An umbrella review 数字摄影和人工智能辅助图像解释在龋齿检查中的诊断效率：综述

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-11-07 DOI: 10.1016/j.jobcr.2025.10.027

P.D. Madan Kumar , Sasidharan Sivakumar , S. Rajeshwari , Lavanya C , K. Ranganathan

Background

Dental caries, a prevalent oral health issue impacting all demographics globally, necessitates precise detection and evaluation for effective intervention and preventive strategies. Advances in scientific research and technology have facilitated the development of innovative applications that utilize Artificial Intelligence (AI) within the medical sciences. AI offers promising improvements in risk assessment and caries prediction. A comprehensive body of evidence assessing the diagnostic accuracy of AI for identifying Dental caries via digital intraoral photographs remains insufficient.

Aim and objectives

Thus, the aim is to consolidate evidence regarding the efficacy of photographic examination and subsequent image analysis, demonstrating that these methods offer comparable accuracy to traditional visual inspection in the diagnosis of Dental caries.

Methodology

Eight systematic reviews were obtained from Medline, Web of Science, Scopus, and EMBASE up to June 2024. These articles were screened at the title, abstract, and full-text levels, with the risk of bias evaluated using the Joanna Briggs Institute (JBI) assessment tool.

Results

AI-based dental caries detection from intraoral photographs showed sensitivities ranging from 67 % to 96 % and specificities from 75 % to 99.2 % with AUC values reported between 0.74 and 0.987 across modalities. The overall risk of bias was minimal.

Conclusion

Therefore, AI-assisted interpretation of digital photography can significantly aid in the early identification of Dental caries, thereby enhancing patient outcomes.

龋齿是影响全球所有人口的普遍口腔健康问题，需要对其进行精确检测和评估，以制定有效的干预和预防策略。科学研究和技术的进步促进了在医学科学中利用人工智能的创新应用的发展。人工智能在风险评估和龋齿预测方面提供了有希望的改进。评估人工智能通过数字口内照片识别龋齿的诊断准确性的综合证据仍然不足。因此，目的是巩固关于摄影检查和随后的图像分析的有效性的证据，证明这些方法在诊断龋齿方面提供与传统视觉检查相当的准确性。方法截至2024年6月，从Medline、Web of Science、Scopus和EMBASE获取8篇系统评价。这些文章在标题、摘要和全文层面进行筛选，并使用乔安娜布里格斯研究所（JBI）的评估工具评估偏倚风险。结果基于人工智能的口腔内照片龋齿检测灵敏度为67% ~ 96%，特异性为75% ~ 99.2%，各种模式下的AUC值在0.74 ~ 0.987之间。总体偏倚风险最小。因此，人工智能辅助解读数码照片可以显著帮助早期识别龋齿，从而提高患者的治疗效果。

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引用次数: 0

Prevalence and distribution of shoveling and double shoveling non-metric traits in Mysuru population: A cross sectional study Mysuru人群铲铲和双铲非计量性状的流行和分布：一项横断面研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-12-10 DOI: 10.1016/j.jobcr.2025.12.006

G.R. Aiswarya , H.S. Sreeshyla , Swathi Kumareswar

Background

Non-metric dental traits play a pivotal role in anthropological and forensic investigations. Shovel-shaped incisors, in particular, serve as valuable indicators of population affinity and individual identification. This study aimed to assess the frequency and grading of the non-metric shoveling and double shoveling traits in the Mysuru population using the Arizona State University Dental Anthropology System (ASUDAS) scoring criteria.

Methodology

A cross-sectional descriptive study was conducted in Mysuru, among 382 individuals (190 males and 192 females) aged 18–40 years. Traits were assessed clinically using ASUDAS plaques. Data were recorded and analyzed using descriptive statistics and chi-square tests to determine prevalence and statistical significance across gender, arch, quadrant and tooth type.

Results

Shoveling was highly prevalent in the population (95.5 %), especially in upper central incisors (92.2 %), followed by upper lateral incisors (69.3 %). In contrast, lower anterior teeth exhibited minimal expression (<3 %). Double shoveling was rare, observed in only 1.0 % of individuals. No statistically significant difference in trait prevalence was found between genders or sides (p > 0.05). However, shoveling was significantly more common in the upper arch than the lower (p = 0.000). Grading analysis revealed Grade 1 shoveling as the most common form, particularly in upper central and lateral incisors. Double shoveling was mostly absent, with only trace levels (Grade 1) being noted.

Conclusion

The study highlights the prevalence of shoveling, especially in the maxillary anterior teeth among the Mysuru population. The results support the forensic and anthropological value of these traits in personal identification and population studies.

非计量牙齿特征在人类学和法医调查中发挥着关键作用。尤其是铲形门牙，可以作为种群亲和力和个体识别的有价值指标。本研究旨在使用亚利桑那州立大学牙科人类学系统（ASUDAS）评分标准评估Mysuru人群中非公制铲牙和双铲牙特征的频率和分级。方法采用横断面描述性研究方法，对382名年龄在18-40岁的个体（男性190名，女性192名）进行研究。使用ASUDAS斑块进行临床特征评估。采用描述性统计和卡方检验对数据进行记录和分析，以确定不同性别、牙弓、象限和牙型的患病率和统计学意义。结果人群中铲牙率最高（95.5%），以上中切牙最高（92.2%），其次为上侧切牙（69.3%）。相比之下，下前牙表达最少（< 3%）。双铲是罕见的，仅在1.0%的个体中观察到。各性别、各方面的性状患病率差异无统计学意义（p > 0.05）。然而，铲铲在上弓比下弓明显更常见（p = 0.000）。分级分析显示1级铲牙是最常见的形式，特别是在上中门牙和侧门牙。大多数情况下没有双铲，只有微量水平（1级）被记录。结论该研究突出了Mysuru人群中铲牙的患病率，特别是上颌前牙。研究结果支持了这些特征在个人鉴定和人口研究中的法医和人类学价值。

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引用次数: 0

Evaluation and comparison of the efficacy of the diode laser and Biodentine on dentinal tubule occlusion 二极管激光与百度定治疗牙本质小管闭塞的疗效评价与比较

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-12-11 DOI: 10.1016/j.jobcr.2025.11.014

Zahra Jalalian , Fatemeh Dibaji , Marzieh Rohaninasab

Background & aim

Dental hypersensitivity (DH) is a common issue characterized by discomfort and pain when cold, hot, or acidic substances are consumed. DH treatment aims to control or eliminate causative factors and utilize desensitizing agents to occlude open dentinal tubules; however, there is no standardized definitive treatment yet. The increasing use of lasers, especially diode lasers, along with innovative materials such as Biodentin has contributed to advancing treatments. This study aimed to evaluate and compare the efficacy of a diode laser and Biodentin in the treatment of dentinal tubule occlusion.

Materials & methods

In this experimental study, 48 extracted human teeth were randomly divided into four groups: a control group (no treatment), a Biodentine-treated group, a diode laser 660 nm-treated group, and a combination group treated with both laser and Biodentine. After treatment, the samples were examined by scanning electron microscopy (SEM), and the percentage of tubule occlusion was categorized into four levels.

Results

A total of 83.3 % of the samples in the combination group (laser and Biodentine) achieved level 3 occlusion (more than 75 % occlusion), whereas 91.7 % of those in the Biodentine-only group were also in level 3. The percentage of patients in the laser-only group was 33.3 % in Level 2 and 66.7 % in Level 1 occlusion.

Conclusion

In conclusion, there was no significant difference in the occlusion measurement between the combination treatment group and the Biodentine-only group, with both being primarily rated as Level 3 occlusions. Therefore, the use of a diode laser at 660 nm in conjunction with Biodentine has effects similar to those of Biodentine alone.

背景：心理过敏症（DH）是一种常见的疾病，在食用冷、热或酸性物质时表现为不适和疼痛。DH治疗的目的是控制或消除致病因素，利用脱敏剂封堵开放的牙本质小管；然而，目前还没有标准化的明确治疗方法。越来越多地使用激光器，特别是二极管激光器，以及创新材料，如Biodentin，有助于推进治疗。本研究旨在评估和比较二极管激光和生物牙本质素治疗牙本质小管闭塞的疗效。材料与方法本实验研究将48颗拔除的人牙随机分为4组：对照组（未处理）、Biodentine治疗组、二极管激光660 nm治疗组、激光与Biodentine联合治疗组。治疗后用扫描电镜检查，将小管闭塞率分为4个等级。结果激光联合百奥汀组83.3%的样本达到3级咬合（75%以上），而百奥汀组91.7%的样本也达到3级咬合。仅激光治疗组2级患者的比例为33.3%，1级患者的比例为66.7%。结论综上所述，联合治疗组与单独使用百奥汀组在咬合测量上无显著差异，均初步评定为3级咬合。因此，660nm二极管激光与Biodentine联合使用的效果与单独使用Biodentine的效果相似。

{"title":"Evaluation and comparison of the efficacy of the diode laser and Biodentine on dentinal tubule occlusion","authors":"Zahra Jalalian , Fatemeh Dibaji , Marzieh Rohaninasab","doi":"10.1016/j.jobcr.2025.11.014","DOIUrl":"10.1016/j.jobcr.2025.11.014","url":null,"abstract":"<div><h3>Background & aim</h3><div>Dental hypersensitivity (DH) is a common issue characterized by discomfort and pain when cold, hot, or acidic substances are consumed. DH treatment aims to control or eliminate causative factors and utilize desensitizing agents to occlude open dentinal tubules; however, there is no standardized definitive treatment yet. The increasing use of lasers, especially diode lasers, along with innovative materials such as Biodentin has contributed to advancing treatments. This study aimed to evaluate and compare the efficacy of a diode laser and Biodentin in the treatment of dentinal tubule occlusion.</div></div><div><h3>Materials & methods</h3><div>In this experimental study, 48 extracted human teeth were randomly divided into four groups: a control group (no treatment), a Biodentine-treated group, a diode laser 660 nm-treated group, and a combination group treated with both laser and Biodentine. After treatment, the samples were examined by scanning electron microscopy (SEM), and the percentage of tubule occlusion was categorized into four levels.</div></div><div><h3>Results</h3><div>A total of 83.3 % of the samples in the combination group (laser and Biodentine) achieved level 3 occlusion (more than 75 % occlusion), whereas 91.7 % of those in the Biodentine-only group were also in level 3. The percentage of patients in the laser-only group was 33.3 % in Level 2 and 66.7 % in Level 1 occlusion.</div></div><div><h3>Conclusion</h3><div>In conclusion, there was no significant difference in the occlusion measurement between the combination treatment group and the Biodentine-only group, with both being primarily rated as Level 3 occlusions. Therefore, the use of a diode laser at 660 nm in conjunction with Biodentine has effects similar to those of Biodentine alone.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 235-239"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered taste perception in Type 2 Diabetes Mellitus: A case-control study on taste thresholds and glycemic control 2型糖尿病味觉感知改变：味觉阈值和血糖控制的病例对照研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-11-30 DOI: 10.1016/j.jobcr.2025.11.015

Julika De , Hina Handa , Gaurav Arya , Sanskriti Agrawal , Anirban Das , Swagata Saha

Background and objectives

Diabetes Mellitus (DM) is associated with altered taste perception, often leading to cravings for carbohydrate-rich foods. Although taste dysfunction has been linked to diabetic neuropathy, this association is debatable. Few studies have evaluated taste thresholds in individuals with diabetes, necessitating further investigations. This study aimed to assess gustatory function in patients with Type 2 Diabetes Mellitus (T2DM).

Materials and methods

A total of 138 participants were included in the study: 46 with controlled T2DM, 46 with uncontrolled T2DM, and 46 healthy controls. Taste perception was assessed using whole-mouth threshold and spatial tests. Data were analysed using the Kruskal-Wallis test, followed by Dunn-Bonferroni post hoc tests for pairwise comparisons. The significance level (p) was set at 0.05 for all statistical analyses.

Results

HbA1c levels were highest in the non-controlled diabetes group (11.22%), followed by the controlled DM group (7.43%) and the control group (5.08%) (p < 0.001). Whole-mouth threshold and spatial taste tests showed significant taste impairment in patients with diabetes, worsening with poor glycaemic control (p < 0.001), particularly for sweet, sour, and bitter tastes. Salt perception was unaffected (p = 0.56).

Conclusion

Individuals with diabetes exhibit taste dysfunction, particularly for sweet, sour, and bitter tastes. Blunted sweet perception may lead to increased sugar intake, worsening hyperglycaemia. These findings highlight the need for dietary counselling to mitigate the impact of taste impairment on glycaemic control.

背景与目的糖尿病（DM）与味觉改变有关，通常导致对富含碳水化合物的食物的渴望。虽然味觉功能障碍与糖尿病神经病变有关，但这种联系是有争议的。很少有研究评估糖尿病患者的味觉阈值，这需要进一步的研究。本研究旨在评估2型糖尿病（T2DM）患者的味觉功能。材料和方法本研究共纳入138名参与者：46名T2DM患者，46名未控制T2DM患者，46名健康对照。味觉知觉通过全口阈值和空间测试进行评估。采用Kruskal-Wallis检验分析数据，随后采用Dunn-Bonferroni事后检验进行两两比较。所有统计分析的显著性水平(p)设为0.05。结果非控制糖尿病组患者shba1c水平最高（11.22%），其次为控制糖尿病组（7.43%）和对照组（5.08%）（p < 0.001）。全口阈值和空间味觉测试显示，糖尿病患者有明显的味觉障碍，血糖控制不良会加重（p < 0.001），尤其是甜、酸和苦的味觉。对盐的感知不受影响（p = 0.56）。结论糖尿病患者存在味觉功能障碍，尤其是甜、酸、苦味觉功能障碍。甜味感知迟钝可能导致糖摄入量增加，加剧高血糖。这些发现强调了饮食咨询的必要性，以减轻味觉障碍对血糖控制的影响。

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引用次数: 0

SFXN1 expression analysis in oral squamous cell carcinoma and its association with the PI3K-AKT-mTOR pathway and immune cell infiltration SFXN1在口腔鳞状细胞癌中的表达分析及其与PI3K-AKT-mTOR通路和免疫细胞浸润的关系

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-11-25 DOI: 10.1016/j.jobcr.2025.11.010

Prabhu Manickam Natarajan , Manoj Kumar Karuppan Perumal , Sudhir Rama Varma , Sam Thomas Kuriadom , Ruba Odeh , Remya Rajan Renuka

Background

Oral squamous cell carcinoma (OSCC) is a predominant malignancy characterized by aggressive progression and poor prognosis. This study investigated the role of SFXN1 (Sideroflexin 1), a mitochondrial serine transporter, in OSCC using integrated bioinformatic and experimental approaches.

Objective

To analyze the expression, clinical relevance, and functional associations of SFXN1 in OSCC through comprehensive bioinformatic and in vitro investigations.

Methods

Transcriptomic data from TCGA pan-cancer cohorts were analyzed to evaluate SFXN1 expression patterns. The expression levels in OSCC were validated using KB OC cell lines, with clinical correlations assessed for tumor grade, nodal status, and patient survival. Immune infiltration associations and protein‒protein interaction networks were constructed, followed by pathway enrichment analyses. Experimental validation was performed via in vitro assays.

Results

The results revealed that SFXN1 was significantly overexpressed in head and neck squamous cell carcinoma and markedly upregulated in KB cells compared with controls. SFXN1 expression was associated with tumor grade and nodal metastasis, although no significant stage-specific differences were observed. Survival analysis revealed no statistically significant association with overall survival. Immune infiltration analysis indicated modest but significant correlations between SFXN1 and immune cell populations, particularly CD4⁺ T cells. Protein network analysis identified hub genes, including AKT1, BCL2, MTOR, and CASP3. Pathway enrichment implicated SFXN1 is involved in the PI3K-AKT-mTOR and p53 signaling pathways.

Conclusion

These findings highlight the involvement of SFXN1 in cancer-related pathways and its potential role in OSCC, suggesting potential therapeutic targeting opportunities that need further investigation.

背景：口腔鳞状细胞癌（OSCC）是一种主要的恶性肿瘤，其特点是进展积极，预后差。本研究采用生物信息学和实验相结合的方法研究了线粒体丝氨酸转运蛋白SFXN1 （Sideroflexin 1）在OSCC中的作用。目的通过综合生物信息学和体外研究，分析SFXN1在OSCC中的表达、临床意义和功能关联。方法分析来自TCGA泛癌症队列的转录组学数据，评估SFXN1表达模式。使用KB OC细胞系验证OSCC中的表达水平，并评估肿瘤分级、淋巴结状态和患者生存率的临床相关性。构建免疫浸润关联和蛋白-蛋白相互作用网络，然后进行途径富集分析。通过体外分析进行实验验证。结果与对照组相比，SFXN1在头颈部鳞状细胞癌中显著过表达，在KB细胞中显著上调。SFXN1表达与肿瘤分级和淋巴结转移有关，但未观察到明显的分期特异性差异。生存分析显示与总生存无统计学意义的关联。免疫浸润分析显示SFXN1与免疫细胞群，特别是CD4+ T细胞之间存在适度但显著的相关性。蛋白网络分析鉴定出枢纽基因，包括AKT1、BCL2、MTOR和CASP3。通路富集暗示SFXN1参与PI3K-AKT-mTOR和p53信号通路。结论这些发现突出了SFXN1参与癌症相关通路及其在OSCC中的潜在作用，提示潜在的治疗靶向机会需要进一步研究。

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引用次数: 0

High expression of TRMT112 is associated with the development of oral squamous cell carcinoma TRMT112的高表达与口腔鳞状细胞癌的发展有关

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2026-01-06 DOI: 10.1016/j.jobcr.2025.12.014

Anitha Pandi , Premkumar Rajendhiran , Vijayashree Priyadharsini Jayaseelan , Paramasivam Arumugam

Background

TRMT112 is a member of the transfer RNA (tRNA) methyltransferase family, and its dysregulation in humans is involved in carcinogenesis. This study aimed to investigate the expression and clinical significance of TRMT112 in patients with oral squamous cell carcinoma (OSCC).

Materials and methods

Quantitative real-time PCR (qPCR) and Western blot were used to analyze TRMT112 expression in paired tumor and non-tumor tissues of OSCC. Furthermore, we analyzed TRMT112 expression for clinicopathological features, prognosis, immune infiltration, and immunotherapy response using the TCGA-HNSCC datasets, which primarily include OSCC data through UALCAN, Human Protein Atlas, Kaplan-Meier plots, and TIMER2.0. The oncogenic role and mechanism of TRMT112 were analyzed using a functional enrichment approach.

Results

TRMT112 expression was significantly upregulated in OSCC tissues compared to non-tumor tissues. The upregulated expression of TRMT112 was associated with advanced tumor stages, metastasis, lower immune infiltrating levels, immunotherapy resistance, and worse prognosis. Protein network and functional pathway enrichment analysis revealed that TRMT112 interacts with well-known oncoproteins that play a critical role in oral cancer progression.

Conclusions

Overall, our novel findings revealed that TRMT112 is associated with the oncogenic process of OSCC, which suggests that TRMT112 could serve as a potential prognostic and therapeutic candidate.

trmt112是转移RNA （tRNA）甲基转移酶家族的一员，其在人类中的失调与致癌有关。本研究旨在探讨TRMT112在口腔鳞状细胞癌（OSCC）患者中的表达及临床意义。材料与方法采用实时荧光定量PCR （quantitative real-time PCR, qPCR）和Western blot技术分析TRMT112在OSCC配对肿瘤组织和非肿瘤组织中的表达。此外，我们使用TCGA-HNSCC数据集分析了TRMT112表达与临床病理特征、预后、免疫浸润和免疫治疗反应的关系，该数据集主要包括通过UALCAN、Human Protein Atlas、Kaplan-Meier图和TIMER2.0获得的OSCC数据。利用功能富集法分析了TRMT112的致癌作用和机制。结果与非肿瘤组织相比，strmt112在OSCC组织中的表达明显上调。TRMT112表达上调与肿瘤分期、转移、免疫浸润水平降低、免疫治疗耐药、预后差相关。蛋白网络和功能通路富集分析显示，TRMT112与已知的在口腔癌进展中起关键作用的癌蛋白相互作用。总之，我们的新发现揭示了TRMT112与OSCC的致癌过程有关，这表明TRMT112可能作为潜在的预后和治疗候选基因。

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引用次数: 0

Evaluation of chlorogenic acids and melanoidin interactions with salivary proteins and their effect on tooth discoloration 评价绿原酸和类黑素与唾液蛋白的相互作用及其对牙齿变色的影响

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-11-14 DOI: 10.1016/j.jobcr.2025.11.006

Trianna Wahyu Utami , Muhammad Raka Aqila Zaky , Margareta Rinastiti , Diatri Nari Ratih , Sri Budi Barunawati , Nunuk Purwanti , Yosi Bayu Murti

Background

Tooth discoloration is a multifactorial process that is significantly influenced by the interaction between dietary polyphenols and salivary proteins. This study investigates coffee-induced discoloration using in vitro staining models and in silico simulations.

Materials and methods

For 14 days, human premolar teeth were soaked in coffee solution (27 g/450 mL) with daily replacement. Lab and ΔE measurements were used to quantify color changes before and after immersion using ultraviolet–visible spectroscopy. Melanoidin compounds docked with salivary proteins (statherin, histatin, and proline-rich protein [PRP]). Hex 8.0 was used for molecular docking, whereas Discovery Studio Visualizer and UCSF Chimera were used for molecular dynamics analysis.

Results

Molecular docking revealed the strong binding affinity of chlorogenic acid with PRP (−251.66 kJ/mol), histatin (−245.4 kJ/mol), and statherin (−240.5 kJ/mol), stabilized by hydrogen bonds with glycine/arginine/tyrosine residues. The molecular dynamics simulations confirmed complex stability with statherin (RMSD 4.2–4.6 Å) and histatin (RMSD 4.4–4.6 Å). Chromameter analysis showed significant color changes after coffee exposure (ΔEab = 12.286 ± 3.645), with a decrease in lightness (L: 83.569 → 71.873) and an increase in redness (a*: 1.375 → 2.992) and yellowness (b*: 15.848 → 18.585). Paired samples correlation analysis revealed statistically significant changes in the a* and b* values (p < 0.001), while the L* value showed no significant change (p = 0.257).

Conclusion

Chlorogenic acid drives discoloration through stable protein interactions and measurable color shifts, suggesting that targeted inhibition of these molecular pathways could prevent coffee-induced discoloration.

背景：牙齿变色是一个多因素的过程，主要受膳食多酚和唾液蛋白相互作用的影响。本研究使用体外染色模型和计算机模拟来研究咖啡诱导的变色。材料与方法将人前磨牙用咖啡溶液（27 g/450 mL）浸泡14 d，每天更换一次。实验室和ΔE测量使用紫外可见光谱学来量化浸泡前后的颜色变化。类黑素化合物与唾液蛋白（脂肪蛋白、组蛋白和富含脯氨酸的蛋白[PRP]）对接。使用Hex 8.0进行分子对接，使用Discovery Studio Visualizer和UCSF Chimera进行分子动力学分析。结果分子对接显示绿原酸与PRP（−251.66 kJ/mol）、组蛋白（−245.4 kJ/mol）和石脂蛋白（−240.5 kJ/mol）具有较强的结合亲和力，并通过与甘氨酸/精氨酸/酪氨酸残基的氢键稳定。分子动力学模拟证实了与startherin （RMSD 4.2-4.6 Å）和组蛋白（RMSD 4.4-4.6 Å）的复合物稳定性。色度分析显示，咖啡暴露后颜色变化显著（ΔEab = 12.286±3.645），亮度降低（L: 83.569→71.873），红度增加（a*: 1.375→2.992），黄度增加（b*: 15.848→18.585）。配对样本相关分析显示，a*和b*值变化有统计学意义（p < 0.001）， L*值变化无统计学意义（p = 0.257）。结论绿原酸通过稳定的蛋白质相互作用和可测量的颜色变化来驱动变色，表明有针对性地抑制这些分子途径可以预防咖啡诱导的变色。

{"title":"Evaluation of chlorogenic acids and melanoidin interactions with salivary proteins and their effect on tooth discoloration","authors":"Trianna Wahyu Utami , Muhammad Raka Aqila Zaky , Margareta Rinastiti , Diatri Nari Ratih , Sri Budi Barunawati , Nunuk Purwanti , Yosi Bayu Murti","doi":"10.1016/j.jobcr.2025.11.006","DOIUrl":"10.1016/j.jobcr.2025.11.006","url":null,"abstract":"<div><h3>Background</h3><div>Tooth discoloration is a multifactorial process that is significantly influenced by the interaction between dietary polyphenols and salivary proteins. This study investigates coffee-induced discoloration using in vitro staining models and in silico simulations.</div></div><div><h3>Materials and methods</h3><div>For 14 days, human premolar teeth were soaked in coffee solution (27 g/450 mL) with daily replacement. Lab and ΔE measurements were used to quantify color changes before and after immersion using ultraviolet–visible spectroscopy. Melanoidin compounds docked with salivary proteins (statherin, histatin, and proline-rich protein [PRP]). Hex 8.0 was used for molecular docking, whereas Discovery Studio Visualizer and UCSF Chimera were used for molecular dynamics analysis.</div></div><div><h3>Results</h3><div>Molecular docking revealed the strong binding affinity of chlorogenic acid with PRP (−251.66 kJ/mol), histatin (−245.4 kJ/mol), and statherin (−240.5 kJ/mol), stabilized by hydrogen bonds with glycine/arginine/tyrosine residues. The molecular dynamics simulations confirmed complex stability with statherin (RMSD 4.2–4.6 Å) and histatin (RMSD 4.4–4.6 Å). Chromameter analysis showed significant color changes after coffee exposure (ΔEab = 12.286 ± 3.645), with a decrease in lightness (L: 83.569 → 71.873) and an increase in redness (a*: 1.375 → 2.992) and yellowness (b*: 15.848 → 18.585). Paired samples correlation analysis revealed statistically significant changes in the a* and b* values (p < 0.001), while the L* value showed no significant change (p = 0.257).</div></div><div><h3>Conclusion</h3><div>Chlorogenic acid drives discoloration through stable protein interactions and measurable color shifts, suggesting that targeted inhibition of these molecular pathways could prevent coffee-induced discoloration.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 1","pages":"Pages 93-100"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145527620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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