Journal of oral biology and craniofacial research最新文献_第8页

Comparative efficacy of autologous platelet-rich fibrin and collagen membrane on clinical outcomes following vestibuloplasty: A randomized controlled trial 自体富血小板纤维蛋白和胶原膜对前庭成形术后临床结果的比较疗效：一项随机对照试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-11-11 DOI: 10.1016/j.jobcr.2025.10.021

Udita Sharma , Prajakta Rao , Sneh Agrawal , Ruhi Mohta , Rachel R. Changrani , Ritika Kriplani

Background

Adequate vestibular depth and attached gingiva are essential for successful periodontal and prosthetic rehabilitation. Vestibuloplasty is frequently performed to correct shallow vestibules; however, relapse due to scar formation remains a concern. Autologous platelet-rich fibrin (PRF) and collagen membranes are increasingly explored as biological wound dressing materials to improve healing outcomes. This randomized controlled trial compared the clinical efficacy of PRF and collagen membrane following vestibuloplasty.

Materials and methods

Thirty-four systemically healthy patients requiring mandibular anterior vestibuloplasty were randomly allocated into two groups: Group A (PRF, n = 17) and Group B (collagen membrane, n = 17). A standardized surgical protocol and postoperative regimen were followed. Clinical parameters assessed included postoperative pain (Visual Analog Scale), wound healing (Landry Healing Index), vestibular depth (VD), and width of attached gingiva (WAG) at baseline, Day 7, Day 15, 1 month, and 3 months. Statistical analysis was performed using Mann–Whitney U test for intergroup comparisons, and Friedman's test with Wilcoxon signed-rank test for intragroup comparisons. Significance was set at p < 0.05.

Results

PRF significantly reduced postoperative pain at all evaluated time points compared to collagen membrane (p < 0.05). Collagen membrane demonstrated superior wound healing scores on Day 15 and at 3 months (p < 0.05), with comparable outcomes between groups at 1 month. WAG increased significantly in both groups, with no intergroup differences. However, Group B exhibited significantly greater gains in VD at both 1 month (p = 0.002) and 3 months (p = 0.001).

Conclusion

PRF is advantageous in controlling immediate postoperative pain, while collagen membrane provides superior long-term wound healing and vestibular depth augmentation. Both materials are equally effective in maintaining attached gingiva width. The selection of dressing material should be guided by the clinician's primary objective—whether prioritizing postoperative comfort or enhancing regenerative outcomes.

背景：足够的前庭深度和附着的牙龈是成功的牙周修复和修复必不可少的。前庭成形术常用于矫正浅前庭；然而，由于疤痕形成而复发仍然是一个问题。自体富血小板纤维蛋白（PRF）和胶原膜越来越多地被探索作为生物伤口敷料材料，以提高愈合效果。这项随机对照试验比较了前庭成形术后PRF和胶原膜的临床疗效。材料与方法34例全身性健康的下颌前庭成形术患者随机分为两组：A组（PRF, n = 17）和B组（胶原膜，n = 17）。遵循标准化的手术方案和术后治疗方案。临床参数评估包括术后疼痛（视觉模拟量表）、伤口愈合（Landry愈合指数）、前庭深度（VD）和附着龈宽度（WAG）在基线、第7天、第15天、第1个月和第3个月。组间比较采用Mann-Whitney U检验，组内比较采用Friedman检验，组内比较采用Wilcoxon符号秩检验。p <； 0.05为显著性。结果与胶原膜相比，sprf在所有评估时间点均显著减轻了术后疼痛（p < 0.05）。胶原膜在第15天和第3个月表现出更好的伤口愈合评分（p < 0.05）， 1个月时两组之间的结果具有可比性。两组WAG均显著升高，组间差异无统计学意义。然而，B组在1个月（p = 0.002）和3个月（p = 0.001）时VD均有显著增加。结论prf在控制术后即刻疼痛方面具有优势，而胶原膜在创面长期愈合和前庭深度增加方面具有优势。两种材料在维持附着牙龈宽度方面同样有效。敷料的选择应以临床医生的主要目标为指导-是否优先考虑术后舒适度或提高再生结果。

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引用次数: 0

Detection of clinically relevant resistance genes in subgingival biofilms of chronic periodontitis: A cross-sectional molecular surveillance study 慢性牙周炎龈下生物膜中临床相关耐药基因的检测：一项横断面分子监测研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-12-09 DOI: 10.1016/j.jobcr.2025.12.002

Krishnasamy Nitya , K. Hema Shree , Aishwarya Arya , Sameep Shetty

Background

Antimicrobial resistance (AMR) has emerged as a major barrier to effective periodontal therapy, particularly when adjunctive antibiotics are prescribed empirically. The oral cavity—especially the subgingival biofilm—serves as a dynamic reservoir for antibiotic resistance genes (ARGs), yet data from the Indian population remain limited.

Aim

To detect and characterize the presence and co-occurrence of clinically relevant ARGs—tet(M), blaTEM, and erm(B)—within subgingival biofilms of Indian patients with chronic periodontitis through PCR-based molecular surveillance.

Methods

This cross-sectional pilot study (n = 20) was conducted among systemically healthy adults diagnosed with chronic periodontitis (2018 AAP-EFP criteria). The sample size was pragmatically defined to assess feasibility and prevalence trends. Subgingival plaque from the deepest pocket per patient (non-pooled) was collected before instrumentation by a single calibrated examiner. DNA was extracted and subjected to end-point PCR targeting tet(M), blaTEM, and erm(B), with gene-specific positive and no-template negative controls included in each run. Amplification reproducibility was verified through cross-laboratory concordance testing. Descriptive and Fisher's exact analyses were applied to explore gender-wise patterns.

Results

tet(M) was detected in 65 % of patients, blaTEM in 45 %, and erm(B) in 30 %. Dual-gene co-occurrence was observed in 35 %, while 20 % harbored all three genes. The most frequent association was tet(M) + blaTEM. Males exhibited a higher mean gene burden (1.8 ± 0.4) than females (1.4 ± 0.5), though differences were not statistically significant (p > 0.05).

Conclusion

This pilot demonstrates a substantial prevalence and co-occurrence of resistance determinants in periodontal biofilms, underscoring the oral cavity's role in local and systemic AMR propagation. While preliminary, these findings support incorporating molecular resistance surveillance into periodontal diagnostics and tailoring region-specific antibiotic stewardship strategies to preserve therapeutic efficacy.

抗生素耐药（AMR）已成为有效牙周治疗的主要障碍，特别是当辅助抗生素处方经验。口腔——尤其是龈下生物膜——是抗生素耐药基因（ARGs）的动态储存库，然而来自印度人群的数据仍然有限。目的通过基于聚合酶链反应（pcr）的分子监测，检测和表征印度慢性牙周炎患者龈下生物膜中临床相关的ARGs-tet (M)、blaTEM和erm(B) -的存在和共现情况。方法本横断面试点研究（n = 20）在诊断为慢性牙周炎（2018年AAP-EFP标准）的全身健康成人中进行。实际定义样本量以评估可行性和流行趋势。每位患者（非合并）最深牙袋的龈下菌斑在仪器前由一名校准的检查员收集。提取DNA，并对tet(M)、blaTEM和erm(B)进行终点PCR，每次运行均包括基因特异性阳性对照和无模板阴性对照。通过跨实验室一致性测试验证扩增重复性。描述性和Fisher的精确分析被应用于探索性别模式。结果65%的患者检出stet(M)， 45%的患者检出blaTEM， 30%的患者检出erm(B)。双基因共现者占35%，三种基因共现者占20%。最常见的关联是tet(M) + blaTEM。男性的平均基因负荷（1.8±0.4）高于女性（1.4±0.5），但差异无统计学意义（p > 0.05）。结论：该试验表明牙周生物膜中耐药决定因素的普遍存在和共同发生，强调了口腔在局部和全身AMR传播中的作用。虽然是初步的，但这些发现支持将分子耐药性监测纳入牙周诊断和定制特定区域的抗生素管理策略，以保持治疗效果。

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引用次数: 0

Tomatidine suppresses PI3K/Akt signaling to induce apoptosis in oral squamous cell carcinoma: An in vitro and molecular docking study 番茄碱抑制PI3K/Akt信号诱导口腔鳞状细胞癌细胞凋亡的体外与分子对接研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2026-01-01 Epub Date: 2025-12-11 DOI: 10.1016/j.jobcr.2025.12.007

Sadhana Padmanabhan , Palati Sinduja , Monal Yuwanati , Selvaraj Jayaraman , Senthilmurugan Mullainathan

Introduction

Oral squamous cell carcinoma (OSCC) has high mortality. Over the decades, there has been not substantial improvement in overall survival which is mostly attributed to lack of effective anticancer agent. Tomatidine, a steroidal alkaloid derived and sourced from unripe green tomatoes, has shown immense anticancer potential. However, its activity in OSCC remains largely uncharacterized. This research aims to evaluate the cytotoxic and pro-apoptotic effects of tomatidine in OSCC.

Method

Target gene for Tomatidine on OSCC treatment was analyzed in the TCGA-HNSC dataset. Two gene were prioritized for network pharmacology to establish their relevance, followed by cheminformatics, drug–target screening, molecular docking, and ADME-T profiling to identify lead compounds. Gene expression and overall survival for target proteins (PI3K/AKT signaling) were examined using GEPIA an cBioportal databases. Functional validation for tomatidine was performed using KB cells via cell viability assay to assess the anticancer effect.

Results

Tomatidine treatment reduced the viability of KB cells in a dosage-dependent manner. The docking simulations showed good binding affinities of tomatidine to PI3K (−8.4 kcal/mol), AKT (−8.8), and PTEN (−10.1), suggesting that tomatidine has a potential ability to disrupt PI3K/Akt signaling and apoptosis.

Conclusion

Tomatidine has potent anticancer effects against OSCC cells inhibiting the PI3K/Akt signaling pathway and promoting apoptosis. These findings highlight the tomatidine utility as a natural anticancer compound.

口腔鳞状细胞癌（OSCC）死亡率高。几十年来，由于缺乏有效的抗癌药物，总体生存率并没有显著提高。番茄碱是一种从未成熟的绿色番茄中提取的甾体生物碱，具有巨大的抗癌潜力。然而，它在OSCC中的活动在很大程度上仍然是未知的。本研究旨在探讨番茄碱在OSCC中的细胞毒性和促凋亡作用。方法在TCGA-HNSC数据集中分析番茄碱治疗OSCC的靶基因。首先对两个基因进行网络药理学分析以确定其相关性，然后进行化学信息学、药物靶标筛选、分子对接和ADME-T分析以确定先导化合物。使用GEPIA和cBioportal数据库检测靶蛋白（PI3K/AKT信号通路）的基因表达和总生存期。利用KB细胞，通过细胞活力测定对番茄碱的抗癌作用进行功能验证。结果替丁对KB细胞的杀伤作用呈剂量依赖性。对接模拟显示，番茄碱对PI3K（−8.4 kcal/mol）、AKT（−8.8 kcal/mol）和PTEN（−10.1 kcal/mol）具有良好的结合亲和力，表明番茄碱具有破坏PI3K/ AKT信号通路和细胞凋亡的潜在能力。结论番茄碱对OSCC细胞具有明显的抗癌作用，可抑制PI3K/Akt信号通路，促进细胞凋亡。这些发现突出了番茄碱作为一种天然抗癌化合物的效用。

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引用次数: 0

Development and validation of an AI-based application for early detection and risk stratification of oral potentially malignant disorders 开发和验证基于人工智能的口腔潜在恶性疾病的早期检测和风险分层应用

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-10-28 DOI: 10.1016/j.jobcr.2025.10.017

Akash Gajanan Prabhune , Vinay R. Srihari , Shreya Shree , Manish Katiyar , Vipin Thampi

Background

Oral Potentially Malignant Disorders (OPMDs) are early indicators of oral cancer, and timely detection is essential for improving patient outcomes. However, diagnosis often relies on expert clinical evaluation, which may not be available in low-resource settings.

Objective

This study presents the development and validation of PRAYAAS, an AI-based mobile application for early detection and risk stratification of OPMDs using intraoral images.

Methods

A total of 794 intraoral images were classified into three categories: (1) Normal mucosa/inflammatory conditions, (2) Premalignant conditions, and (3) Oral carcinoma. Images were split into training (70 %), validation (18 %), and test (12 %) datasets while maintaining class balance. Preprocessing involved resizing to 224 × 224 pixels, contrast enhancement, and normalization. A U-Net-based model segmented lesion regions, followed by classification using a fine-tuned DenseNet201 model. Model performance was evaluated using accuracy, precision, recall, F1-score, and confusion matrices.

Results

The DenseNet201 classifier achieved 94 % accuracy on the test set. For normal/inflammatory lesions, precision and recall were 1.00. For premalignant lesions, precision was 0.87 and recall was 1.00. For carcinoma, precision was 1.00 and recall was 0.80. The integrated segmentation module improved lesion focus and reduced background noise. The app provides class-wise risk scores and a user-friendly interface for clinical support.

Conclusion

PRAYAAS offers a robust, mobile-enabled solution for early OPMD screening. By integrating segmentation and classification into a single platform, the tool holds promise for enhancing community-based oral cancer detection and referral.

背景：口腔潜在恶性疾病（OPMDs）是口腔癌的早期指标，及时发现对于改善患者预后至关重要。然而，诊断往往依赖于专家的临床评估，这在资源匮乏的环境中可能无法获得。目的开发并验证基于人工智能的移动应用程序PRAYAAS，通过口腔内图像进行opmd的早期检测和风险分层。方法将794张口腔内图像分为3类：(1)粘膜/炎症正常；(2)癌前病变；(3)口腔癌。图像被分成训练（70%）、验证（18%）和测试（12%）数据集，同时保持类平衡。预处理包括调整大小为224 × 224像素，对比度增强和规范化。基于u - net的模型对病变区域进行分割，然后使用微调的DenseNet201模型进行分类。使用准确性、精密度、召回率、f1分数和混淆矩阵来评估模型的性能。结果DenseNet201分类器在测试集上的准确率达到94%。对于正常/炎性病变，准确率和召回率为1.00。对于癌前病变，准确率为0.87，召回率为1.00。对于癌，准确率为1.00，召回率为0.80。集成的分割模块提高了病灶聚焦，降低了背景噪声。该应用程序提供分类风险评分和临床支持的用户友好界面。结论：prayaas为早期OPMD筛查提供了一个强大的、可移动的解决方案。通过将分割和分类整合到单一平台中，该工具有望加强社区口腔癌的检测和转诊。

{"title":"Development and validation of an AI-based application for early detection and risk stratification of oral potentially malignant disorders","authors":"Akash Gajanan Prabhune , Vinay R. Srihari , Shreya Shree , Manish Katiyar , Vipin Thampi","doi":"10.1016/j.jobcr.2025.10.017","DOIUrl":"10.1016/j.jobcr.2025.10.017","url":null,"abstract":"<div><h3>Background</h3><div>Oral Potentially Malignant Disorders (OPMDs) are early indicators of oral cancer, and timely detection is essential for improving patient outcomes. However, diagnosis often relies on expert clinical evaluation, which may not be available in low-resource settings.</div></div><div><h3>Objective</h3><div>This study presents the development and validation of PRAYAAS, an AI-based mobile application for early detection and risk stratification of OPMDs using intraoral images.</div></div><div><h3>Methods</h3><div>A total of 794 intraoral images were classified into three categories: (1) Normal mucosa/inflammatory conditions, (2) Premalignant conditions, and (3) Oral carcinoma. Images were split into training (70 %), validation (18 %), and test (12 %) datasets while maintaining class balance. Preprocessing involved resizing to 224 × 224 pixels, contrast enhancement, and normalization. A U-Net-based model segmented lesion regions, followed by classification using a fine-tuned DenseNet201 model. Model performance was evaluated using accuracy, precision, recall, F1-score, and confusion matrices.</div></div><div><h3>Results</h3><div>The DenseNet201 classifier achieved 94 % accuracy on the test set. For normal/inflammatory lesions, precision and recall were 1.00. For premalignant lesions, precision was 0.87 and recall was 1.00. For carcinoma, precision was 1.00 and recall was 0.80. The integrated segmentation module improved lesion focus and reduced background noise. The app provides class-wise risk scores and a user-friendly interface for clinical support.</div></div><div><h3>Conclusion</h3><div>PRAYAAS offers a robust, mobile-enabled solution for early OPMD screening. By integrating segmentation and classification into a single platform, the tool holds promise for enhancing community-based oral cancer detection and referral.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1806-1812"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors influencing the retention rate of resin based pit and fissure sealant among primary school children- A randomised clinical trial 影响小学生树脂基窝沟封闭剂保留率的因素-一项随机临床试验

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-08-20 DOI: 10.1016/j.jobcr.2025.08.010

Aruna Vishwakarma , Prashanth Vishwakarma , Shruti Pundkar , Anoli Agrawal

Background

In modern dentistry, the focus is more on preventing caries than treating them, which helps preserve tooth structure. There is an approximately 50 % prevalence of caries on the occlusal surface in school children. Pits and fissures with irregularities and invasions are responsible for this. In dentistry, to overcome these problems, different sealant technique is introduced, for instance, the conventional method, using a bur, using air abrasion, etc. Therefore, this clinical trial was designed to evaluate the retention rate of sealant using different application techniques.

Methodology

The present Randomised clinical trial included 50 children aged 6–9 from a primary school children based on inclusion and exclusion criteria. The contralateral 1st mandibular molar teeth were divided randomly before placing sealant with two different techniques.

Results

The results obtained showed that there was a statistically highly significant difference in mean scores at the end of the 1st, 3rd, 9th, and 12th months of pit and fissure sealant application done with altered morphology without the use of bonding agent compared to altered morphology with bonding agent.

Conclusion

The present study showed that sealant retention was higher in the altered morphology without bonding agent group compared to the altered morphology with bonding agent.

在现代牙科中，预防龋齿比治疗龋齿更重要，这有助于保护牙齿结构。在学龄儿童中，大约有50%的龋发生在牙合面。不规则和侵入的凹坑和裂缝是造成这种情况的原因。在牙科中，为了克服这些问题，引入了不同的密封技术，例如传统的方法，使用bur，使用空气磨损等。因此，本临床试验旨在评估不同应用技术下密封剂的保留率。方法本随机临床试验根据纳入和排除标准纳入了50名6-9岁的小学儿童。采用两种不同的方法将对侧下颌第一磨牙随机分开，然后放置密封胶。结果结果显示，在使用牙槽和裂隙密封剂的第1、3、9和12个月结束时，未使用粘结剂的形态改变组与使用粘结剂的形态改变组的平均评分有显著的统计学差异。结论形态学改变组的密封胶固位率高于形态学改变组。

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引用次数: 0

Whitlockite as a next-generation biomaterial for bone regeneration: A systematic review of In Vivo evidence for bone regeneration. Whitlockite作为下一代骨再生生物材料：骨再生体内证据的系统回顾。

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-08-06 DOI: 10.1016/j.jobcr.2025.08.002

Sinduja Palati, Dhanraj Ganapathy, Saravanan Sekaran

Background: Whitlockite (WH), a magnesium-enriched calcium phosphate mineral, is emerging as a promising biomaterial in bone tissue engineering due to its chemical similarity to natural bone and dual role in promoting osteogenesis and regulating bone resorption. Compared to conventional materials like hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), WH offers higher solubility, superior ion release (notably Mg²⁺), and enhanced bioactivity.

Objective: This systematic review evaluates the in vivo efficacy of WH-based biomaterials in bone regeneration. Key outcomes include bone volume fraction (BV/TV), bone mineral density (BMD), osteogenic marker expression, and histological bone quality.

Methods: A comprehensive search of PubMed, Web of Science, Google Scholar, and Cochrane Central was conducted up to March 2025. Eligible studies assessed WH-based materials in animal bone defect models with quantifiable regenerative outcomes. Two reviewers independently performed data extraction and quality assessment using the SYRCLE Risk of Bias tool. Meta-analysis was not feasible due to significant heterogeneity across models, scaffold types, and endpoints.

Results: Seventeen animal studies (rats, mice, rabbits) met inclusion criteria. WH was used in forms such as nanoparticles, granules, and scaffolds with polymers like chitosan and gelatin. WH consistently outperformed HA and β-TCP with up to a 2-6 % increase in BV/TV, BMD, and histological bone formation. Upregulation of ALP, OCN, RUNX2, and COL1 was observed. Doped WH variants and composites enhanced osteoinductive and angiogenic responses. No adverse effects were reported.

Conclusion: WH demonstrates superior osteogenic and biocompatible properties over traditional calcium phosphates. Future standardized, long-term studies are needed to support clinical translation for orthopedic and dental bone regeneration.

背景：惠特洛克石（Whitlockite， WH）是一种富镁磷酸钙矿物，具有与天然骨相似的化学性质和促进骨生成和调节骨吸收的双重作用，是一种很有前途的骨组织工程生物材料。与羟基磷灰石（HA）和β-磷酸三钙（β-TCP）等传统材料相比，WH具有更高的溶解度，更好的离子释放（特别是Mg2+）和增强的生物活性。目的：系统评价骨再生生物材料在体内的应用效果。主要结果包括骨体积分数（BV/TV）、骨矿物质密度（BMD）、成骨标志物表达和组织学骨质量。方法：综合检索PubMed、Web of Science、谷歌Scholar和Cochrane Central，检索截止至2025年3月。符合条件的研究评估了wh基材料在动物骨缺损模型中的可量化再生结果。两名审稿人使用sycle偏倚风险工具独立进行数据提取和质量评估。由于模型、支架类型和终点的显著异质性，meta分析是不可行的。结果：17项动物研究（大鼠、小鼠、家兔）符合纳入标准。WH以纳米颗粒、颗粒和壳聚糖和明胶等聚合物的支架等形式使用。WH持续优于HA和β-TCP，在BV/TV、骨密度和组织学骨形成方面增加了2- 6%。ALP、OCN、RUNX2、COL1表达上调。掺杂WH变体和复合材料增强了骨诱导和血管生成反应。无不良反应报告。结论：水灵比传统的磷酸钙具有更好的成骨和生物相容性。未来需要标准化的长期研究来支持骨科和牙科骨再生的临床转化。

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引用次数: 0

Dual-action platelet-rich fibrin in periodontal therapy: Assessing the efficacy of injectable versus metronidazole-enhanced platelet-rich fibrin in non-surgical periodontal treatment - A clinical study. 双重作用富血小板纤维蛋白在牙周治疗：评估注射与甲硝唑增强富血小板纤维蛋白在非手术牙周治疗中的疗效-一项临床研究。

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-08-07 DOI: 10.1016/j.jobcr.2025.07.028

Devadharshini Chandrasekar, Burnice Nalina Kumari Chellathurai, Jaideep Mahendra, Vijayalakshmi Rajaram

Background: Adjunctive therapies play a crucial role in enhancing the efficacy of non-surgical periodontal therapy (NSPT) by addressing the multifactorial nature of periodontal disease. Injectable platelet-rich fibrin (i-PRF) and metronidazole-infused PRF gel have emerged as potential biomaterials that promote periodontal regeneration and antibacterial effects, respectively.

Objective: To evaluate and compare the clinical efficacy of i-PRF and metronidazole-infused PRF gel as adjuncts to NSPT in patients with periodontitis.

Method: ology: A randomized controlled trial was conducted on 20 periodontal sites in patients with Stage II-III periodontitis. Sites were divided into two groups (n = 10 each), receiving either i-PRF or metronidazole-infused PRF gel following NSPT. Clinical parameters, including Oral Hygiene Index (OHI), Gingival Index (GI), Bleeding on Probing (BOP), Probing Pocket Depth (PPD), and Clinical Attachment Level (CAL), were assessed at baseline, 4 weeks, and 3 months. Statistical analyses included the Mann-Whitney U test for intergroup comparisons and the Friedman test for intragroup comparisons.

Results: Both groups showed significant improvement in PPD and CAL over the study period (p < 0.001). The metronidazole-infused PRF gel group demonstrated a more pronounced reduction in GI and BOP compared to the i-PRF group (p = 0.01 and p = 0.66, respectively). Improvements in OHI were observed in both groups but were not statistically significant.

Conclusion: The study highlights the potential of metronidazole-infused PRF gel as a superior adjunct to NSPT due to its enhanced antimicrobial effects and periodontal tissue healing properties. Personalized therapeutic strategies incorporating bioactive materials can optimize periodontal treatment outcomes.

背景：通过解决牙周病的多因素特性，辅助治疗在提高非手术牙周治疗（NSPT）的疗效方面起着至关重要的作用。可注射的富血小板纤维蛋白（i-PRF）和注入甲硝唑的PRF凝胶已分别成为促进牙周再生和抗菌作用的潜在生物材料。目的：评价并比较i-PRF与甲硝唑灌注PRF凝胶联合NSPT治疗牙周炎的临床疗效。方法：对ⅱ~ⅲ期牙周炎患者20个牙周部位进行随机对照试验。试验点分为两组（每组10个），分别在NSPT后接受i-PRF或注射甲硝唑PRF凝胶。临床参数包括口腔卫生指数（OHI）、牙龈指数（GI）、探诊出血（BOP）、探诊袋深度（PPD）和临床依恋水平（CAL），分别在基线、4周和3个月进行评估。统计分析包括组间比较的Mann-Whitney U检验和组内比较的Friedman检验。结果：在研究期间，两组患者的PPD和CAL均有显著改善(p结论：该研究强调了甲硝唑注入PRF凝胶作为NSPT的优越辅助物的潜力，因为它具有增强的抗菌作用和牙周组织愈合性能。结合生物活性材料的个性化治疗策略可以优化牙周治疗效果。

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引用次数: 0

Assessment of efficacy of lignocaine patch in pain management post third molar extraction: A prospective, split mouth study 评估利多卡因贴片在第三磨牙拔牙后疼痛管理中的疗效：一项前瞻性的裂口研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-09-24 DOI: 10.1016/j.jobcr.2025.09.016

P.S. Tripthi, Rakshita Kumar, Padmaraj Hegde

Introduction

Postoperative pain following third molar extractions is a significant concern, often managed using opioids and NSAIDs, associated with considerable side effects. Lignocaine patches, known for their localized action and minimal systemic effects, have shown promise in managing various pain conditions. This study aimed to evaluate the efficacy of 5 % lignocaine patches in reducing postoperative pain following third molar extractions.

Methodology

A prospective, split-mouth study was conducted on 30 patients aged 18–40 years requiring bilateral extraction of impacted mandibular third molars. Post extraction on one side (test lignocaine patches were applied postoperatively, while post extraction of contralateral side, standard oral analgesics were given. Pain levels were assessed using the Visual Analog Scale (VAS) on the day of extraction, postoperative day (POD) 1 and POD 2. Additional patches or analgesics were provided if pain exceeded VAS thresholds.

Results

The test group reported significantly lower mean VAS scores (1.96 ± 0.72) compared to the control group (2.55 ± 0.35, p = 0.004). Significant reduction in pain was observed in both groups over time, but the test group showed greater pain relief by POD 2 (p = 0.000) and no major adverse effects were reported, only 13.3 % experienced inadequate analgesia, requiring the need of a second patch.

Conclusion

These findings suggest that 5 % lignocaine patches offer an effective alternative to oral analgesics for managing postoperative pain after third molar extractions. Further research, however, is recommended to explore their potential in reducing opioid use and their effectiveness in other minor oral surgical procedures.

第三磨牙拔牙后的术后疼痛是一个值得关注的问题，通常使用阿片类药物和非甾体抗炎药进行治疗，并伴有相当大的副作用。利多卡因贴片，以其局部作用和最小的全身作用而闻名，在治疗各种疼痛状况方面显示出希望。本研究旨在评估5%利多卡因贴片减少第三磨牙拔牙术后疼痛的疗效。方法对30例年龄18-40岁需要双侧拔除下颌阻生第三磨牙的患者进行前瞻性、口裂研究。术后一侧拔牙后应用试验用利多卡因贴片，对侧拔牙后给予标准口服镇痛药。采用视觉模拟评分法（VAS）评估拔牙当天、术后第1天（POD）和第2天（POD）的疼痛程度。如果疼痛超过VAS阈值，则提供额外的贴片或镇痛药。结果试验组VAS平均评分（1.96±0.72）明显低于对照组（2.55±0.35,p = 0.004）。随着时间的推移，两组的疼痛都有明显的减轻，但试验组显示POD 2的疼痛缓解更大（p = 0.000），没有报告主要的不良反应，只有13.3%的人经历了不充分的镇痛，需要第二次贴片。结论5%利多卡因贴片是治疗第三磨牙拔牙术后疼痛的有效替代药物。然而，建议进一步研究它们在减少阿片类药物使用方面的潜力及其在其他小型口腔外科手术中的有效性。

{"title":"Assessment of efficacy of lignocaine patch in pain management post third molar extraction: A prospective, split mouth study","authors":"P.S. Tripthi, Rakshita Kumar, Padmaraj Hegde","doi":"10.1016/j.jobcr.2025.09.016","DOIUrl":"10.1016/j.jobcr.2025.09.016","url":null,"abstract":"<div><h3>Introduction</h3><div>Postoperative pain following third molar extractions is a significant concern, often managed using opioids and NSAIDs, associated with considerable side effects. Lignocaine patches, known for their localized action and minimal systemic effects, have shown promise in managing various pain conditions. This study aimed to evaluate the efficacy of 5 % lignocaine patches in reducing postoperative pain following third molar extractions.</div></div><div><h3>Methodology</h3><div>A prospective, split-mouth study was conducted on 30 patients aged 18–40 years requiring bilateral extraction of impacted mandibular third molars. Post extraction on one side (test lignocaine patches were applied postoperatively, while post extraction of contralateral side, standard oral analgesics were given. Pain levels were assessed using the Visual Analog Scale (VAS) on the day of extraction, postoperative day (POD) 1 and POD 2. Additional patches or analgesics were provided if pain exceeded VAS thresholds.</div></div><div><h3>Results</h3><div>The test group reported significantly lower mean VAS scores (1.96 ± 0.72) compared to the control group (2.55 ± 0.35, <em>p</em> = 0.004). Significant reduction in pain was observed in both groups over time, but the test group showed greater pain relief by POD 2 (<em>p</em> = 0.000) and no major adverse effects were reported, only 13.3 % experienced inadequate analgesia, requiring the need of a second patch.</div></div><div><h3>Conclusion</h3><div>These findings suggest that 5 % lignocaine patches offer an effective alternative to oral analgesics for managing postoperative pain after third molar extractions. Further research, however, is recommended to explore their potential in reducing opioid use and their effectiveness in other minor oral surgical procedures.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1601-1606"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shade determination based on digital photographs and different sources of illumination. An in-vivo, pilot study 基于数码照片和不同光源的阴影测定。一项体内试验研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-09-23 DOI: 10.1016/j.jobcr.2025.09.020

Srishty Pundir, Siddharth Swarup, V.N.V. Madhav

Aim

The aim of the study was to compare shade estimation in maxillary anterior teeth under different ambient light sources, whilst using digital photography.

Materials and methods

In this study different ambient light conditions were considered for shade determination. 96 readings were obtained from six maxillary anterior teeth were selected for this study, and shade was determined at the middle 1/3rd of the tooth using a digital spectrophotometer and the L∗a∗b∗values obtained, which was the control. Photographs were taken as per the different light sources. The photographs were then subjected to a Classic Color Meter software to derive the L∗a∗b∗ values. The L∗a∗b∗ values obtained were statistically analysed and results obtained.

Results

The results depicted that cool white light as compared to the digital spectrophotometer showed better results with statistically insignificant difference in the L∗ category (p = 0.598) & b category (p = 0.143). Warm white light was also comparable only in the L∗ category (p = 0.903). Natural sunlight showed similar results when compared in the L∗ category (p = 0.939), but varied significantly in a∗ and b∗ categories. While LED Ceiling light showed the most significantly different results under all categories of L∗, a∗ and, b∗.

Conclusion

It was concluded that digital photography method of shade estimation can be a reliable method of shade estimation under cool white light illuminance. Warm white light and natural sunlight are dependable measures for value matching, whereas LED ceiling lights yielded inaccurate shades through the digital photography method of shade estimation in all axes of the CIELAB space.

目的利用数码摄影技术，比较不同环境光源下上颌前牙的阴影估计。材料和方法在本研究中考虑了不同的环境光条件来确定阴影。本研究选取6颗上颌前牙的96个读数，用数字分光光度计测定牙齿中间1/3处的色度，得到L∗a∗b∗值，作为对照。照片是根据不同的光源拍摄的。然后将照片置于经典色计软件中以推导出L * a * b *值。对所得的L∗a∗b∗值进行统计分析并得出结果。结果冷白光与数字分光光度计相比，在L *类（p = 0.598）和b类（p = 0.143）中显示出更好的结果，差异无统计学意义。温白光也仅在L *类别中具有可比性（p = 0.903）。自然光照在L *类别中表现出相似的结果（p = 0.939），但在a *和b *类别中变化显著。而LED吸顶灯在L∗、a∗和b∗的所有类别下的结果差异最显著。结论在冷白光条件下，数字摄影的阴影估计方法是一种可靠的阴影估计方法。暖白光和自然光是价值匹配的可靠措施，而LED吸顶灯通过数字摄影方法在CIELAB空间的所有轴上进行阴影估计，产生了不准确的阴影。

{"title":"Shade determination based on digital photographs and different sources of illumination. An in-vivo, pilot study","authors":"Srishty Pundir, Siddharth Swarup, V.N.V. Madhav","doi":"10.1016/j.jobcr.2025.09.020","DOIUrl":"10.1016/j.jobcr.2025.09.020","url":null,"abstract":"<div><h3>Aim</h3><div>The aim of the study was to compare shade estimation in maxillary anterior teeth under different ambient light sources, whilst using digital photography.</div></div><div><h3>Materials and methods</h3><div>In this study different ambient light conditions were considered for shade determination. 96 readings were obtained from six maxillary anterior teeth were selected for this study, and shade was determined at the middle 1/3rd of the tooth using a digital spectrophotometer and the L∗a∗b∗values obtained, which was the control. Photographs were taken as per the different light sources. The photographs were then subjected to a Classic Color Meter software to derive the L∗a∗b∗ values. The L∗a∗b∗ values obtained were statistically analysed and results obtained.</div></div><div><h3>Results</h3><div>The results depicted that cool white light as compared to the digital spectrophotometer showed better results with statistically insignificant difference in the L∗ category (p = 0.598) & b category (p = 0.143). Warm white light was also comparable only in the L∗ category (p = 0.903). Natural sunlight showed similar results when compared in the L∗ category (p = 0.939), but varied significantly in a∗ and b∗ categories. While LED Ceiling light showed the most significantly different results under all categories of L∗, a∗ and, b∗.</div></div><div><h3>Conclusion</h3><div>It was concluded that digital photography method of shade estimation can be a reliable method of shade estimation under cool white light illuminance. Warm white light and natural sunlight are dependable measures for value matching, whereas LED ceiling lights yielded inaccurate shades through the digital photography method of shade estimation in all axes of the CIELAB space.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 6","pages":"Pages 1573-1578"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative clinical characterization of microflora between primary and secondary endodontic infections-An in vivo cross sectional study 原发性和继发性牙髓感染的微生物群临床特征比较-一项体内横断面研究

Q1 Medicine

Journal of oral biology and craniofacial research

Pub Date : 2025-11-01 Epub Date: 2025-09-23 DOI: 10.1016/j.jobcr.2025.09.018

Mahantesh Yeli , Balaram Naik , RaghavendraD. Kulkarni , Vignesh Kamath , Prashant Moogi , Shruti Patil , Kishore Bhat

Aim

The aim of the present study was to analyse the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.

Materials and methodology

The experimental material included the samples collected from 139 patients. These subjects were divided into two groups of 74 and 65 patients. Group 1-Primary endodontic infections, Group 2-Secondary endodontic infections (Retreatment cases). The collected samples were subjected to aerobic and anaerobic bacterial cultures and selected specific uncultivable microorganisms were subjected to Polymerase Chain Reaction. The material collected in transport medium was mixed thoroughly and it was divided into two aliquots. One of the aliquots was used for aerobic culture and Polymerase Chain Reaction, the other was subjected to anaerobic culture study. Statistical analysis was done. P < 0.05 was considered as statistically significant.

Results

For the given sample, the values for Porphyromonas gingivalis (p = 0.001), Prevotella intermedia (p = 0.017) and Streptococcus aerobic (p = 0.001), Klebsiella aerogens (p = 0.03) and Streptococcus (p = 0.005) and Dialister invisus (p = 0.024) showed statistically significant differences between primary and secondary lesions.

Conclusions

The primary lesions showed significantly higher number of Klebsiella aerogens, Streptococcus, Dialister invisus and secondary lesions with Porphyromonas gingivalis, Prevotella intermedia, Streptococcus respectively.

目的本研究的目的是分析接受根管治疗的患者的原发性和继发性/持续性根管感染的微生物群，以及临床和放射学表现。材料和方法实验材料包括139例患者的样本。这些受试者被分为两组，每组74名和65名患者。组1-原发性牙髓感染，组2-继发性牙髓感染（再治疗病例）。收集的样品进行好氧和厌氧细菌培养，并选择特定的不可培养微生物进行聚合酶链反应。将收集到的物料在输送介质中充分混合，并分成两等分。其中一份用于好氧培养和聚合酶链反应，另一份用于厌氧培养研究。进行统计分析。P <； 0.05认为有统计学意义。结果原发性和继发性病变中牙龈卟啉单胞菌（p = 0.001）、中间普雷沃菌（p = 0.017）、需氧链球菌（p = 0.001）、克雷伯菌（p = 0.03）、链球菌（p = 0.005）和内溶Dialister （p = 0.024）的检出率差异均有统计学意义。结论原发性病变中肺克雷伯菌、链球菌、内隐Dialister数量显著高于原发性病变，继发性病变中分别有牙龈卟啉单胞菌、中间普雷沃菌、链球菌。

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引用次数: 0