Journal of photochemistry and photobiology. B, Biology最新文献_第8页

Multifaceted impacts of UVB radiation on morphology, cellular dynamics, and behavior in Macrobrachium olfersii larvae UVB辐射对奥氏沼虾（Macrobrachium olfersii）幼虫形态、细胞动力学和行为的多方面影响

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-26 DOI: 10.1016/j.jphotobiol.2025.113289

Giuliam Kátia Strücker, Madson Silveira de Melo, Evelise Maria Nazari

The incidence of ultraviolet B (UVB) radiation on Earth's surface has been increasing, impacting marine, coastal, and freshwater organisms. Although these organisms may possess molecular, cellular, and behavioral mechanisms to mitigate the effects of UVB radiation, the embryos and larvae of many species lack fully developed protective strategies. In this context, Macrobrachium olfersii is a decapod essential to the energy cycling of aquatic environments. M. olfersii inhabits shallow and clear coastal waters and is, therefore, susceptible to UVB exposure. This study aimed to investigate the complex effects of UVB radiation on M. olfersii larvae by comparing their responses at two stages of exposure—embryonic and post-hatching—across various levels of cellular, morphological, and behavioral damage. Specifically, we examined whether UVB-induced damage in embryos persists into larval development (first set) and whether irradiated larvae exhibit responses to UVB similar to those observed in irradiated embryos (second set). In the first set of experiments, the parameters of larval body length, eye index, and proteins associated with mitophagy were affected. In the second set, these parameters were impacted, along with more pronounced DNA damage, cell proliferation, and apoptosis. Furthermore, both experimental sets decreased larval migration toward illuminated fields, while cell transparency decreased only in the second set. The results reveal carryover effects of UVB radiation on M. olfersii development, with early exposure prompting adaptation and later exposure causing physiological and functional impairments, potentially reducing predator evasion, survival, and reproductive success.

地球表面紫外线B （UVB）辐射的发生率一直在增加，影响海洋、沿海和淡水生物。尽管这些生物可能具有减轻UVB辐射影响的分子、细胞和行为机制，但许多物种的胚胎和幼虫缺乏充分发展的保护策略。在这种情况下，奥氏沼虾是水生环境能量循环必不可少的十足动物。M. olfersii生活在浅水和清澈的沿海水域，因此很容易受到中波紫外线的照射。本研究旨在通过比较UVB辐射对M. olfersii幼虫在暴露于胚胎和孵化后两个阶段的不同水平的细胞、形态和行为损伤的反应，探讨UVB辐射对M. olfersii幼虫的复杂影响。具体来说，我们研究了UVB诱导的胚胎损伤是否会持续到幼虫发育（第一组），以及辐照后的幼虫对UVB的反应是否与辐照后的胚胎相似（第二组）。在第一组实验中，影响了幼虫体长、眼指数和与有丝分裂相关的蛋白质等参数。在第二组中，这些参数受到影响，同时出现更明显的DNA损伤、细胞增殖和凋亡。此外，两组实验都减少了幼虫向光照场的迁移，而细胞透明度仅在第二组实验中降低。研究结果揭示了UVB辐射对M. olfersii发育的携带效应，早期暴露促进适应，后期暴露导致生理和功能损伤，可能降低捕食者的躲避、生存和繁殖成功率。

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引用次数: 0

Mimicking exogenous amino acids: Raman-based and machine learning tracking of Colon cancer cells metabolism 模拟外源性氨基酸：基于拉曼和机器学习的结肠癌细胞代谢跟踪

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-22 DOI: 10.1016/j.jphotobiol.2025.113285

Monika Kopeć, Karolina Beton-Mysur, Arkadiusz Jarota, Beata Brożek-Płuska

Amino acids are fundamental biomolecules that constitute the building blocks of proteins. Monitoring their dysregulation and fluctuations serves as an important indicator of human health. The aim of this study is to explore the role of exogenous amino acids metabolism in colon cancer development. Raman spectroscopy and Raman imaging were employed to investigate biochemical changes in normal human colon cells (CCD-18Co) and cancerous colon cells (Caco-2), both untreated and supplemented with leucine, threonine, and arginine. Spectroscopic data were analyzed using chemometric methods, specifically Partial Least Squares Discriminant Analysis (PLS-DA). Raman spectroscopy, combined with chemometric approach, identified unique Raman biomarkers – 1088/1262, 1444/1660, 1580/1004, and 1630/1444 - that correspond to changes in nucleic acids, lipids/lipids+Amide I, proteins, and lipids, respectively, in both normal and cancer cells supplemented with amino acids. These findings underscore the significant potential of Raman spectroscopy and Raman imaging as powerful tools for investigating the role of exogenous amino acids in colon cancer progression. We have also shown that the S₁-state lifetime of tetra-sulphonated aluminum phthalocyanine remains unaffected by amino acid supplementation, as confirmed by femtosecond transient absorption spectroscopy, supporting its stability as a photosensitizer for photodynamic therapy.

氨基酸是构成蛋白质基石的基本生物分子。监测它们的失调和波动是人类健康的重要指标。本研究旨在探讨外源性氨基酸代谢在结肠癌发生发展中的作用。采用拉曼光谱和拉曼成像技术研究了未处理和补充亮氨酸、苏氨酸和精氨酸的正常人结肠细胞（CCD-18Co）和癌变结肠细胞（Caco-2）的生化变化。光谱数据分析使用化学计量学方法，特别是偏最小二乘判别分析（PLS-DA）。拉曼光谱与化学计量学方法相结合，鉴定出独特的拉曼生物标志物- 1088/1262、1444/1660、1580/1004和1630/1444 -分别对应于补充氨基酸的正常细胞和癌细胞中核酸、脂质/脂质+酰胺I、蛋白质和脂质的变化。这些发现强调了拉曼光谱和拉曼成像作为研究外源氨基酸在结肠癌进展中的作用的强大工具的巨大潜力。我们还表明，经飞秒瞬态吸收光谱证实，四磺化酞青铝的S₁态寿命不受氨基酸补充的影响，支持其作为光动力治疗的光敏剂的稳定性。

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引用次数: 0

LED light emission and olive pomace-rich culture medium in potentiating bacterial cellulose synthesis and improving biopolymer properties LED发光和橄榄果渣培养基在增强细菌纤维素合成和改善生物聚合物性能方面的应用

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-21 DOI: 10.1016/j.jphotobiol.2025.113287

Paula Rodrigues , Halima Khelifa , Tatiana B. Schreiner , Noureddine Halla , Maria Filomena F. Barreiro , Pedro J.L. Crugeira

The growing demand for sustainable and high-performance materials has driven research into optimizing bacterial cellulose (BC) production using innovative strategies. In this context, this work aimed to evaluate the enhancement of BC biosynthesis by Komagataeibacter intermedius, combining LED photobiomodulation with a natural olive pomace-based medium (20 % MOP) and their impact on the properties of the produced BC. Hestrin-Schram medium was used as the control. LED irradiation (630 nm) was applied at 14 J/cm² (control LED; 20 % MOP LED) in static culture for 7 days at 28 °C. The MOP medium and LED emission demonstrated a synergistic effect, resulting in a significant production increase of 385.97 % compared to the control. The BC membranes synthesized in the 20 % MOP and LED irradiation exhibited improved thermal stability, stress capacity (247.06 %), and strain at break (33.96 %) compared to the control. The production-to-quality ratio, the valorization of the by-product, and the use of the energy-efficient emitter are key factors for the scalability and viability of the bioprocess and its industrial application.

对可持续和高性能材料的需求不断增长，推动了利用创新策略优化细菌纤维素（BC）生产的研究。在此背景下，本研究旨在评估将LED光生物调节与天然橄榄果浆培养基（20% MOP）相结合，对Komagataeibacter intermedius生物合成BC的促进作用，以及它们对所产BC性质的影响。以Hestrin-Schram培养基为对照。以14 J/cm2（对照LED， 20% MOP LED）照射630 nm，在28°C下静态培养7天。MOP介质与LED发光表现出协同效应，产量较对照显著提高385.97%。与对照组相比，在20% MOP和LED照射下合成的BC膜具有更好的热稳定性、应力承受能力（247.06%）和断裂应变（33.96%）。生产质量比、副产品的价值和节能发射器的使用是生物工艺的可扩展性和可行性及其工业应用的关键因素。

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引用次数: 0

Plasmonic photothermal based quick sterilization of surgical tools and pathogen suspensions 基于等离子光热的手术工具和病原体悬浮液的快速灭菌

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-21 DOI: 10.1016/j.jphotobiol.2025.113286

Sarabjot Kaur , Parminder Kaur , Shobit Attery , Vrushali Raka , Rajneesh Dadwal , Hemraj Nandanwar , Sanjeev Soni

This study investigates the efficacy of plasmonic photothermal technique for quick sterilization of surgical tools and pathogen suspensions for the elimination of a high load of broad-spectrum pathogens viz. ESKAPE pathogens - Candida albicans (5 × 10⁷ CFU/mL of each pathogen). Here, the contaminated surgical tools and the corresponding pathogens suspension to be sterilized, are irradiated with electromagnetic radiation (Visible-NIR) in the presence of synthesized triangular silver nanoplates leading to localized heat generation through plasmonic photothermal phenomenon. The irradiation source including the sterilization device prototype is developed In-house and is used for the sterilization of reusable surgical tools. The sterilization is achieved at lower temperatures of ∼65–70 °C, at atmospheric pressure within 10 min of total duration. Also, sterility of the nanoplates suspension after a treatment cycle is confirmed for its re-use for multiple subsequent sterilization cycles. The demonstrated sterilization efficacy ∼7.69 Log₁₀ reductions of each pathogen, meets the required sterility assurance level (SAL) norms of 6 Log₁₀ reduction. Further, the mechanism behind sterilization efficacy of plasmonic photothermal technique shows the morphological alterations & membrane lysis along with the combined impact of reactive oxygen species (ROS), which disrupts cellular metabolic pathways within the cells. In-vivo investigation of the use of a surgical blade sterilized through plasmonic photothermal technique, on BALB/C mice, further strengthens the real-time application and safety. Contrary to the antimicrobial studies reported in literature for such phenomenon, as far as we are aware, it is the first reported instance of the sterilization for reusable surgical tools through plasmonic photothermal technique.

本研究探讨了等离子光热技术对手术工具和病原体悬浮液快速灭菌的效果，以消除高负荷的广谱病原体，即ESKAPE病原体-白色念珠菌（每种病原体5 × 107 CFU/mL）。在这里，被污染的手术工具和相应的待消毒的病原体悬浮液，在合成的三角形纳米银片存在的情况下，用电磁辐射（可见光-近红外）照射，通过等离子体光热现象产生局部热。包括灭菌装置原型在内的辐照源是内部开发的，用于可重复使用的手术工具的灭菌。灭菌在较低的温度下（~ 65-70°C），在大气压下，总持续时间为10分钟。此外，在处理周期后，纳米片悬浮液的无菌性被确认为可用于多个后续灭菌周期。结果表明，每种病原体的灭菌效率降低了7.69 Log10，达到了无菌保证水平（SAL）要求的6 Log10降低标准。此外，等离子光热技术杀菌效果背后的机制显示了形态改变和膜裂解以及活性氧（ROS）的综合影响，这破坏了细胞内的细胞代谢途径。等离子光热技术灭菌手术刀片在BALB/C小鼠体内的应用研究，进一步加强了实时性和安全性。与文献报道的这种现象的抗菌研究相反，据我们所知，这是第一个通过等离子光热技术对可重复使用的手术工具进行灭菌的报道。

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引用次数: 0

Polyphenol-rich green propolis extract improves antioxidant and UV protection in sunscreen formulations 富含多酚的绿色蜂胶提取物在防晒配方中提高抗氧化和紫外线防护

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-17 DOI: 10.1016/j.jphotobiol.2025.113284

Michelli Ferrera Dario , Flávia Sobreira Gonçalves , Axelle Sogan , Yanka Rodrigues Cardoso Barbosa , Mariana Mandelli de Almeida , Mauro Luciano Baesso , Monique de Souza , Fabio Galeotti , Nicola Volpi , André Rolim Baby , Maria Valéria Robles Velasco

The potential adverse effects, including systemic reactions, of some organic filters used in sunscreens have driven the search for alternatives. Brazilian green propolis, rich in polyphenols, shows promise in absorbing ultraviolet (UV) radiation and acting as an antioxidant in photoprotective formulations, allowing for a reduction in the concentration of organic filters while maintaining photoprotection efficacy. Oil-in-water emulsions were prepared containing chemical UV filters—ethylhexyl methoxycinnamate and butyl methoxydibenzoylmethane—with or without the addition of Brazilian green propolis extract at concentrations of 2.5 % and 5.0 % (w/w), along with a blank formulation as control. These formulations were assessed for antioxidant activity, photoprotective efficacy (in vitro and in vivo methods), photostability, inhibition of lipid peroxidation in the stratum corneum of human volunteers, and skin permeability. The addition of green propolis extract significantly enhanced both the antioxidant activity and sun protection factor (SPF) of the formulations without significant penetration, making it an excellent alternative to partially replace organic filters. In vitro photoprotective efficacy parameters met broad-spectrum sunscreen standards, providing good to excellent ultraviolet A (UVA) protection. However, the low photostability of the formulations limited the extract's ability to protect the stratum corneum from lipid peroxidation. Therefore, Brazilian green propolis serves as a promising adjuvant in sunscreen formulation, boosting efficacy while ensuring formulation safety.

防晒霜中使用的一些有机过滤器的潜在副作用，包括全身反应，促使人们寻找替代品。富含多酚的巴西绿色蜂胶在吸收紫外线（UV）辐射和光防护配方中充当抗氧化剂方面表现出了希望，可以在保持光防护效果的同时降低有机过滤器的浓度。以空白配方为对照，分别添加2.5%和5.0% （w/w）的巴西绿蜂胶提取物，制备含有化学紫外线过滤器（甲氧基肉桂酸乙基己基和甲氧基二苯甲酰甲烷）的水包油乳液。对这些配方进行了抗氧化活性、光保护功效（体外和体内方法）、光稳定性、对人类志愿者角质层脂质过氧化的抑制以及皮肤渗透性的评估。绿色蜂胶提取物的添加显著提高了配方的抗氧化活性和防晒系数（SPF），而不显着渗透，使其成为部分替代有机过滤器的理想替代品。体外光防护效能参数符合广谱防晒标准，提供良好至优异的紫外线防护。然而，配方的低光稳定性限制了提取物保护角质层免受脂质过氧化的能力。因此，巴西绿蜂胶在防晒配方中是一种很有前途的佐剂，在保证配方安全的同时提高功效。

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引用次数: 0

Comparative efficacy and safety of traditional single-shot and two successive pass and diascopy method in Q-switched Nd: YAG laser-assisted tattoo removal: an evaluator-blinded trial q开关Nd: YAG激光辅助纹身去除的传统单次和两次连续传递和复制方法的疗效和安全性比较：一项评估者盲法试验。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-14 DOI: 10.1016/j.jphotobiol.2025.113283

Amirhossein Rahimnia , Ala Ehsani , Mina Koohian Mohammadabadi , Amirhoushang Ehsani , Pedram Nourmohammadpour , Darya Khosravi , Parvaneh Hatami

Background

Various methods for tattoo removal have been employed over the last century, including different methods of laser treatment.

Aim

To compare three different methods of Q-switched laser treatment.

Methods

A prospective, comparative cohort study was carried out on 17 subjects with tattoos. Each tattoo was segmented into three sections. The first part underwent a single application of the with QS Nd:YAG. The second part received two pass of QS Nd:YAG treatment with a 15-min interval at each session. The third part received a laser treatment with a 4 mm thick silicone blade on the skin (diascopy).

Results

The study included 17 participants aged 18 to 37 years, with an average age of 26.41 ± 9.64 years. Males represented 94.1 % (n = 16) of the sample, while females accounted for 5.9 % (n = 1). The highest clearance degree was achieved by two-shot laser method (3.47 ± 0.68), followed by one-shot laser method (2.72 ± 0.62) and diascopy method (2.45 ± 0.86). Although the clearance degree of two-shot method was significantly higher than one-shot method (P value: 0.014), one-shot and diascopy methods did not show any significant difference in this regard (P value: 0.11). There was no significant difference between mean pain severity expressed by patients in the three treatment methods. None of the patients showed any side effects after treatment.

Conclusion

We found that using two passes of laser treatment in one session enhanced the clinical efficacy, with comparable safety profile to standard one-shot method or diascopy method.

背景：在上个世纪，人们采用了多种方法去除纹身，包括不同的激光治疗方法。目的比较三种不同的调q激光治疗方法。方法：对17名纹身者进行前瞻性、比较队列研究。每个纹身被分成三个部分。第一部分进行了qsnd:YAG的单次应用。第二组接受两组QS Nd:YAG治疗，每组间隔15分钟。第三部分接受激光治疗，在皮肤上使用4毫米厚的硅胶刀片（diascopy）。结果：纳入17例患者，年龄18 ~ 37岁，平均年龄26.41±9.64岁。男性占94.1% (n = 16)，女性占5.9% （n = 1）。两次激光法获得的清除率最高（3.47±0.68），其次是一次激光法（2.72±0.62）和一体机法（2.45±0.86）。虽然两针法的清除程度显著高于一针法（P值：0.014），但一针法与一体机法在这方面没有显著差异（P值：0.11）。三种治疗方法患者表达的平均疼痛严重程度差异无统计学意义。所有患者治疗后均未出现任何副作用。结论：我们发现在一个疗程中使用两道激光治疗可提高临床疗效，其安全性与标准的一次注射方法或一体式手术方法相当。

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引用次数: 0

3, 7-dihydroxy-2, 4-dimethoxyphenanthrene protects against UVB-induced skin hyperpigmentation via antioxidant and anti-melanogenic mechanisms 3,7 -二羟基- 2,4 -二甲氧基菲通过抗氧化和抗黑素机制防止uvb诱导的皮肤色素沉着。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-13 DOI: 10.1016/j.jphotobiol.2025.113277

Mengyan Li , Die Li , Yu Zhang , Juan Wang , Ziying Zhang , Weiqun Wang , Shaochun Chen , Lechun Lyu , Weimin Liu

Background

Excessive reactive oxygen species (ROS) produced by UVB radiation can disrupt the normal redox balance, leading to oxidative cellular damage as well as triggering melanin synthesis by melanocytes. Currently, natural active substances are emphasized in UV protection research. This study explored the protective effect of 3, 7-dihydroxy-2, 4-dimethoxyphenanthrene (DDP), a bioactive compound from Dendrobium lindleyi Stendel, against UVB-induced skin hyperpigmentation and examined its specific mechanism.

Methods

To evaluate the anti- melanogenic and antioxidant activities of DDP in vitro, molecular docking was used to predict the binding conformation of DDP to tyrosinase (TYR) and melanocortin 1 receptor (MC1R). Subsequently, CCK8 and cell live/dead staining were used to screen the safe concentration range. B16-F10 cells were treated with different doses of DDP before UVB light irradiation and before α-melanocyte-stimulating hormone (α-MSH) induction, and the evaluation of cells included determination of melanin content, Western blotting analysis, TYR activity assay, ROS assay, DPPH• and ABTS•⁺ radical scavenging assays. A brown guinea pig model was used to further evaluate the protective effect of DDP on UVB-induced skin pigmentation in vivo, and compared with commercial skin care products.

Results

In the present study, Molecular docking showed that DDP binds to TYR and MC1R better than Kojic Acid. DDP was found to possess excellent anti-melanogenic and antioxidant activities in vitro. DDP was effective in alleviating skin melanin accumulation induced by UVB irradiation without inflammatory side effects.

Conclusions

DDP protects against UVB-induced skin hyperpigmentation via antioxidant and anti-melanogenic mechanisms.

背景：UVB辐射产生过多的活性氧（ROS）会破坏正常的氧化还原平衡，导致细胞氧化损伤，并触发黑色素细胞合成黑色素。目前，天然活性物质是防紫外线研究的重点。本研究探讨了来自林氏石斛的生物活性化合物3,7 -二羟基- 2,4 -二甲氧基菲（DDP）对uvb诱导的皮肤色素沉着的保护作用，并探讨了其具体机制。方法：采用分子对接方法预测DDP与酪氨酸酶（TYR）和黑素皮质素1受体（MC1R）的结合构象，评价DDP的体外抗黑素生成和抗氧化活性。随后采用CCK8和细胞活/死染色法筛选安全浓度范围。在UVB光照射前和α-MSH诱导前分别用不同剂量的DDP处理B16-F10细胞，对细胞的评价包括黑色素含量测定、Western blotting分析、TYR活性测定、ROS测定、DPPH•和ABTS•+自由基清除测定。采用褐豚鼠模型进一步评价DDP对uvb诱导的皮肤色素沉着的体内保护作用，并与市售护肤品进行比较。结果：在本研究中，分子对接显示DDP与TYR和MC1R的结合优于曲酸。体外实验发现DDP具有良好的抗黑色素生成和抗氧化活性。DDP能有效缓解UVB照射引起的皮肤黑色素积累，无炎症副作用。结论：DDP通过抗氧化和抗黑素生成机制对uvb诱导的皮肤色素沉着起到保护作用。

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引用次数: 0

Porphyrin-ligustrazine conjugates: Dual-mode therapeutic agents combining photodynamic and chemotherapy for enhanced antitumor activity 卟啉-川芎嗪缀合物：结合光动力和化疗的双模式治疗剂，增强抗肿瘤活性。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-13 DOI: 10.1016/j.jphotobiol.2025.113282

Lei Shi , Yihui Liu , Zeijie Tian , Chen Tang , LingYan Yang , Ying Fang , Yi Wang , YunMei Liu

Dual-mode therapeutic strategies have demonstrated substantially greater efficacy than single-modality treatments and hold broad clinical potential. In this study, we developed a series of 20 novel ligustrazine–porphyrin derivatives by integrating porphyrin-based photodynamic therapy (PDT) with ligustrazine chemotherapy. UV–vis spectroscopy confirmed that the characteristic photophysical features of porphyrins, including the Soret and Q bands, remained unchanged after conjugation with ligustrazine. Reactive oxygen species (ROS) detection assays revealed that all compounds were capable of generating ROS, with production further enhanced upon coordination with Zn(II). The derivatives exhibited varying levels of antitumor activity against HGC-27, A549, DU145, TCCSUP, HCT-116, and MDA-MB-231 cancer cell lines under both light and dark conditions, while demonstrating relatively lower cytotoxicity toward normal H9C2 cells. Among these compounds, derivative 8d showed the most pronounced antitumor activity.

双模式治疗策略已被证明比单模治疗有效得多，具有广泛的临床潜力。在这项研究中，我们通过将基于卟啉的光动力疗法（PDT）与川芎嗪化疗相结合，开发了一系列20个新的川芎嗪-卟啉衍生物。紫外-可见光谱证实卟啉与川芎嗪偶联后，其特有的Soret和Q波段的光物理特征保持不变。活性氧（ROS）检测分析表明，所有化合物都能产生ROS，与Zn（II）配合后，ROS的产生进一步增强。在光照和黑暗条件下，这些衍生物对HGC-27、A549、DU145、TCCSUP、HCT-116和MDA-MB-231癌细胞均表现出不同程度的抗肿瘤活性，而对正常H9C2细胞的细胞毒性相对较低。其中，衍生物8d的抗肿瘤活性最强。

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引用次数: 0

Wavelength-dependent photobiomodulation attenuates synovial inflammation in fibroblast-like synoviocytes and a collagenase-induced osteoarthritis model 波长依赖的光生物调节减轻成纤维细胞样滑膜细胞的滑膜炎症和胶原酶诱导的骨关节炎模型

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-09 DOI: 10.1016/j.jphotobiol.2025.113276

Tianxiang Fan , Peng Xia , Safayet Ahmed , Yuen H. Tsang , Zhi Yao , Deli Wang , Ziyu Chen , Lixiang Xue , Dongquan Shi , Bo Wang , Chi Fai Cheung , Marco Y.C. Pang , Ye Li , Siu Ngor Fu

Photobiomodulation (PBM) using light-emitting diodes (LEDs) has demonstrated promising anti-inflammatory effects in musculoskeletal disorders. However, the optimal parameters for modulating synovitis and joint degeneration in osteoarthritis (OA) remain unclear. This study aimed to examine the wavelength- and dose-dependent effects of PBM on inflammatory responses in fibroblast-like synoviocytes (FLS) and evaluate the in vivo therapeutic efficacy of selected wavelengths in a murine model of collagenase-induced OA (CIOA). Primary rat FLS were stimulated with TNF-α and irradiated using PBM at 625, 810, 940, or 1050 nm with fluences ranging from 13 to 78 J/cm² (fixed irradiance of 44 mW/cm²). mRNA levels of IL-1β, IL-6, COX-2, and iNOS were quantified by RT-qPCR. The in vivo study involved daily PBM (810 or 940 nm at 39 J/cm²) for two weeks in CIOA mice, starting one week post-induction. Pain-related behavior was assessed weekly using the incapacitance test. Synovial inflammation and cartilage degradation were evaluated by histology and immunofluorescence staining for IL-1β and IL-6. Among tested parameters, 810 nm at 39 J/cm² showed the most potent suppression of inflammatory gene expression in FLS. In vivo, 810 nm PBM significantly improved pain-related behavior by week 4, reduced synovitis and cartilage damage, and downregulated IL-1β and IL-6 expression in synovium. While 940 nm PBM also demonstrated anti-inflammatory effects in vitro, it did not show significant efficacy in vivo. These findings suggest that PBM at 810 nm exerts stronger anti-inflammatory and chondroprotective effects in inflammatory OA compared with 940 nm, supporting its potential as a non-pharmacological intervention for inflammatory OA phenotypes.

使用发光二极管（led）的光生物调节（PBM）在肌肉骨骼疾病中具有良好的抗炎作用。然而，调节骨关节炎（OA）滑膜炎和关节退变的最佳参数仍不清楚。本研究旨在研究PBM对成纤维细胞样滑膜细胞（FLS）炎症反应的波长和剂量依赖效应，并评估选定波长在胶原酶诱导的OA （CIOA）小鼠模型中的体内治疗效果。用TNF-α刺激原代大鼠FLS，并使用PBM在625、810、940或1050 nm照射，影响范围为13至78 J/cm2（固定辐照度为44 mW/cm2）。RT-qPCR检测IL-1β、IL-6、COX-2、iNOS mRNA水平。在体内研究中，从诱导后一周开始，CIOA小鼠每天服用PBM(810或940 nm， 39 J/cm2)，持续两周。疼痛相关行为每周使用失容测试进行评估。采用组织学和免疫荧光法检测IL-1β和IL-6，评价滑膜炎症和软骨降解。在测试参数中，39 J/cm2下810 nm对FLS炎症基因表达的抑制作用最强。在体内，810 nm PBM在第4周显著改善疼痛相关行为，减轻滑膜炎和软骨损伤，并下调滑膜中IL-1β和IL-6的表达。940 nm PBM在体外也有抗炎作用，但在体内没有明显的疗效。这些发现表明，与940 nm相比，810 nm的PBM在炎性OA中具有更强的抗炎和软骨保护作用，支持其作为炎性OA表型非药物干预的潜力。

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引用次数: 0

Photoacidity and solvent-dependent fluorescence of 7,8-didemethyl-8-hydroxy-5-deazariboflavin 7,8-二甲基-8-羟基-5-去氮杂黄素的光酸性和溶剂依赖性荧光。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-10-09 DOI: 10.1016/j.jphotobiol.2025.113280

Elena V. Nemtseva , Dmitry V. Gulnov , Anna A. Deeva , Natalia S. Rodionova , Matvey V. Vavilov , Renata I. Zagitova , Valentin N. Petushkov

7,8-didemethyl-8-hydroxy-5-deazariboflavin (8-HDF or F₀) is a chromophore involved into electronic energy transfer processes in DNA-photolyases, cryptochromes and bioluminescent reaction of the Siberian earthworm, Henlea sp. We studied the impact of the solvent properties (polarity, hydrogen bonding ability, etc.) on F₀ fluorescence using the Catalan multiparametric approach and molecular dynamics technique. Analysis of the absorption and fluorescence spectra, as well as fluorescence decays and decay-associated spectra, of F₀ in 14 solvents revealed the presence of a mixture of protonated and deprotonated forms – F₀H and F₀⁻, respectively, in the majority of solvents. We performed a correlation analysis of the spectral maxima of excitation/emission spectra and fluorescence lifetimes with the Catalan parameters of the solvents. A stronger dependence of spectral maxima of F₀⁻ than of F₀H on the solvent polarizability (SP) was found. For both F₀H and F₀⁻, a weaker effect of solvent basicity (SB) was oppositely directed on excitation and emission spectra, which resulted in a stronger effect on the Stokes shifts. In water solutions with low pH (2.9–4.5), absorption and fluorescence spectra of F₀ indicated the proton transfer during the excited-state lifetime (ESPT). For this process, ΔpK_a = 3.67 ± 0.04 was first obtained, characterizing F₀ as a weak photoacid. All-atom molecular dynamics simulations of F₀H and F₀⁻ in water, methanol, DMSO, and acetone demonstrated that solvent hydrogen-bonding interactions influence the fluorophore's conformational dynamics and could alter F₀ photophysical properties. A special role of the N3 atom as a donor of the hydrogen bonds with solvents or proteins was proposed.

7,8-二甲基-8-羟基-5-脱氮黄素（8-HDF或F0）是一种参与西伯利亚蚯蚓（Henlea sp.） dna光解酶、隐色素和生物发光反应的电子能量传递过程的发色基团。我们利用Catalan多参数方法和分子动力学技术研究了溶剂性质（极性、氢键能力等）对F0荧光的影响。对F0在14种溶剂中的吸收光谱、荧光光谱以及荧光衰减和衰减相关光谱的分析表明，在大多数溶剂中分别存在质子化和去质子化形式F0H和F0-的混合物。我们对激发/发射光谱的光谱最大值和荧光寿命与溶剂的加泰罗尼亚参数进行了相关性分析。发现F0-的光谱最大值比F0H对溶剂极化率（SP）的依赖性更强。对于F0H和F0-，溶剂碱度（SB）对激发光谱和发射光谱的影响较弱，对Stokes位移的影响较强。在低pH（2.9 ~ 4.5）水溶液中，F0的吸收光谱和荧光光谱显示了激发态寿命（ESPT）期间的质子转移。该工艺首先得到ΔpKa = 3.67±0.04，表征F0为弱光酸。F0H和F0-在水、甲醇、DMSO和丙酮中的全原子分子动力学模拟表明，溶剂氢键相互作用影响荧光团的构象动力学，并可能改变F0的光物理性质。提出了N3原子作为溶剂或蛋白质氢键供体的特殊作用。

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引用次数: 0