Journal of photochemistry and photobiology. B, Biology最新文献_第9页

Development and validation of a multi-modal customized device to stimulate in vitro cell culture systems 多模态定制装置的开发和验证，以刺激体外细胞培养系统

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1016/j.jphotobiol.2025.113306

Sofia Oliveira , Francisca Monteiro , Susana O. Catarino , Betina B. Hinckel , Ioannis Sotiropoulos , Ana Leal , Filipe S. Silva , Óscar Carvalho

This work focuses on the development and validation of a multi-modal stimulation device for in vitro cell culture systems. The device was designed to stimulate cells or tissues placed on 12-well culture plates. It is connected to customized software that controls the parameters of photobiomodulation (PBM) and ultrasound stimulation (US) through light-emitting diodes and piezoelectric disks, respectively. A wide range of stimulation protocols can be explored by modulating central frequency or wavelength, power density, and duration. Four different cell lines were used to validate the safety and functionality of the device. Human osteoblasts, chondrocytes, periodontal ligament fibroblasts, and mouse-derived neuronal cells were cultured and stimulated daily with ultrasound (1.0 MHz, 100 mW/cm², 5 min), light (810 nm, 7.5 mW/cm², 5 min) and combined stimuli. After three days, metabolic activity and proliferation were assessed. Different cell types demonstrated distinct biological responses to the stimuli, as osteoblasts and chondrocytes showed increased metabolic activity after combined stimulation or PBM, while the metabolic activity of human fibroblasts or neuronal-like cells was unchanged after three days. This highlights the importance of a rigorous optimization of stimulation protocols according to the target tissue. The safety of the device and its sterilization conditions were demonstrated as there was no cell death or contamination during in vitro stimulation. This work features a feasible, safe, and effective multi-modal stimulation device that can provide a wide range of stimulation protocols to better understand their effect on cells or tissues.

这项工作的重点是开发和验证体外细胞培养系统的多模态刺激装置。该装置旨在刺激放置在12孔培养板上的细胞或组织。它连接到定制的软件，分别通过发光二极管和压电片控制光生物调节（PBM）和超声刺激（US）的参数。通过调制中心频率或波长、功率密度和持续时间，可以探索广泛的刺激方案。使用了四种不同的细胞系来验证该设备的安全性和功能性。培养人成骨细胞、软骨细胞、牙周韧带成纤维细胞和小鼠来源的神经细胞，每天用超声（1.0 MHz, 100 mW/cm2, 5 min）、光（810 nm, 7.5 mW/cm2, 5 min）和联合刺激进行刺激。3天后，评估代谢活性和增殖。不同类型的细胞对刺激表现出不同的生物学反应，如成骨细胞和软骨细胞在联合刺激或PBM后代谢活性增加，而人成纤维细胞或神经元样细胞的代谢活性在三天后保持不变。这突出了根据目标组织严格优化刺激方案的重要性。在体外刺激过程中，没有细胞死亡或污染，证明了该装置的安全性及其灭菌条件。这项工作的特点是一个可行、安全、有效的多模态刺激装置，可以提供广泛的刺激方案，以更好地了解它们对细胞或组织的影响。

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引用次数: 0

Unveiling connectivity and differences of bacterial response mechanisms exposed to multi-radiation through the transcriptomic profiles and physiological characteristics of Mount Everest Sphingomonas radiodurans 珠穆朗玛峰耐辐射鞘单胞菌转录组学和生理特征揭示多重辐射下细菌反应机制的连通性和差异

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-01 Epub Date: 2025-11-08 DOI: 10.1016/j.jphotobiol.2025.113304

Yang Liu , Lu Zhang , Jinxiu Wang , Pengcheng Zhang , Zhuxiang Zhang , Tuo Chen , Guangxiu Liu , Shichang Kang , Wei Zhang , Gaosen Zhang

Radiation-resistant microorganisms employ complex regulatory networks to safeguard cellular protection and DNA repair upon radiation exposure. And, previous studies have focused on a single type of radiation. However, studies specifically exploring the correlations, including connectivity defined as interlinked regulatory networks or coexisted core defense mechanisms, and differences referred to the radiation-type-specific radiation protection and damage repair, between an organism's resistant radiation types and their underlying resistance mechanisms remain limited. Therefore, we conducted an integrated transcriptomic and physiological analysis of Sphingomonas radiodurans from Mount Everest, investigating its connectivity and differences responding to multi-radiations within UVC, γ-ray, and X-ray radiation. For UVC radiation, extracellular polysaccharides reduced direct cell damage, and the RecF homologous recombination pathway was induced to repair DNA DSBs. In response to γ-ray radiation, EPSs also mitigated cell damage; additionally, γ-ray-induced changes in cell membrane proteins and lipids cooperated with EPS to block radiation penetration, and the RecF pathway was activated for DNA DSBs repair. Regarding X-ray radiation, it similarly induced membrane protein and lipid changes to synergize for radiation blocking, but uniquely activated the RecBCD homologous recombination pathway for DNA DSBs repair. Notably, the ROS-scavenging system served as the common connectivity across all three radiation types, mitigating oxidative stress from radiation-induced ROS accumulation. Combined with weighted gene co-expression network analysis, a high proportion of novel genes encoding hypothetical proteins were significantly upregulated in response to multi-radiation. Taken together, these results highlight the coordinated protective strategies of strain S9–5 involving both shared and radiation-specific mechanisms, provide new insights into bacterial response mechanisms of radiation resistance evolution in extreme environments, and serve as important references for developing protection agents against multi-radiation damage.

抗辐射微生物采用复杂的调控网络来保护辐射暴露时的细胞保护和DNA修复。而且，之前的研究都集中在单一类型的辐射上。然而，专门探讨相关性的研究，包括相互关联的调节网络或共存的核心防御机制的连通性，以及生物体耐辐射类型及其潜在抗性机制之间辐射类型特异性辐射防护和损伤修复的差异，仍然有限。因此，我们对来自珠穆朗玛峰的耐辐射鞘单胞菌进行了综合转录组学和生理学分析，研究了其在UVC、γ射线和x射线多重辐射下的连通性和差异。对于UVC辐射，胞外多糖可减少细胞的直接损伤，并可诱导RecF同源重组途径修复DNA dsb。在对γ射线辐射的反应中，eps还能减轻细胞损伤；此外，γ射线诱导的细胞膜蛋白和脂质变化与EPS协同阻止辐射穿透，激活RecF途径修复DNA dsb。在x射线辐射方面，它同样诱导膜蛋白和脂质改变协同作用进行辐射阻断，但它独特地激活了DNA dsb修复的RecBCD同源重组途径。值得注意的是，ROS清除系统作为所有三种辐射类型的共同连接，减轻了辐射诱导的ROS积累引起的氧化应激。结合加权基因共表达网络分析，高比例的编码假设蛋白的新基因在多重辐射下显著上调。综上所述，这些结果突出了菌株S9-5在共享机制和辐射特异性机制下的协同保护策略，为研究极端环境下细菌对辐射抗性进化的响应机制提供了新的见解，并为开发针对多重辐射损伤的防护剂提供了重要参考。

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引用次数: 0

Phage-conjugated chlorin e6: A strategy overcoming phage resistance in biofilm eradication and wound infection therapy of carbapenem-resistant Acinetobacter baumannii 噬菌体共轭氯e6：耐碳青霉烯鲍曼不动杆菌生物膜根除和伤口感染治疗中克服噬菌体耐药性的策略。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-12-01 Epub Date: 2025-11-07 DOI: 10.1016/j.jphotobiol.2025.113302

Jianhui Su , Siyi Chen , Zhishen Huang , Haoxuan He , Huanhuan Zou , Xiaoyi Huang , Yongzhao Xie , Hongxia Zhao , Zhenbo Xu , Tao Lei , Juan Li , Haiyan Zeng

Carbapenem-Resistant Acinetobacter baumannii (CRAB) frequently causes biofilm-related infections that can exhibit extreme resistance to antibiotic therapy. Phage therapy shows promise as an alternative treatment, yet bacteria may develop resistance to it with prolonged use. Phage-photosensitizer combination therapy represents a novel antimicrobial strategy. This study aims to evaluate the efficacy of chlorin e6-functionalized phage in eradicating biofilms and treating CRAB infections, and to assess its sustained after effect following the emergence of phage resistance. The A. baumannii phage (ABP)-chlorin e6 conjugate (ABP-Ce6) was successfully synthesized and characterized. It preserved the phage's absorptive capacity and lytic activity while enhanced reactive oxygen species (ROS) production. Moreover, ABP-Ce6 demonstrated remarkable antibacterial activity comparable to ABP while exceeding that of Ce6, and showed superior performance in both inhibiting biofilm formation and disrupting existing biofilms in CRAB Ab1513. Significantly, although ABP exhibited no efficacy against the phage-resistant CRAB Ab1513-BIM12 due to its inability to achieve irreversible adsorption, the ABP-Ce6 maintained potent antibacterial and biofilm ablation effects against this strain, outperforming free Ce6. This sustained efficacy arises from ABP's reversible adsorption, which still enables proximity-driven Ce6 delivery to the target bacteria. In vivo, the ABP-Ce6 significantly enhanced mice wound healing for infections caused by CRAB Ab1513 and Ab1513-BIM12. In conclusion, ABP-Ce6 exhibits significant efficacy as a therapeutic agent against CRAB infections even after the bacteria develop resistance to phage therapy. This novel strategy may serve as a hopeful complementary strategy to phage therapy, thereby reducing delays in screening for new therapeutic phages.

耐碳青霉烯鲍曼不动杆菌（CRAB）经常引起生物膜相关感染，对抗生素治疗表现出极大的耐药性。噬菌体疗法有望成为一种替代疗法，但细菌可能会因长期使用而产生耐药性。噬菌体-光敏剂联合治疗是一种新的抗菌策略。本研究旨在评价氯e6功能化噬菌体清除生物膜和治疗螃蟹感染的效果，并评估其在噬菌体耐药性出现后的持续后效。成功合成了鲍曼芽胞杆菌噬菌体(ABP)-氯e6偶联物（ABP- ce6）并对其进行了表征。它保留了噬菌体的吸收能力和裂解活性，同时提高了活性氧（ROS）的产生。此外，ABP-Ce6具有与ABP相当且超过Ce6的显著抗菌活性，在抑制螃蟹Ab1513生物膜形成和破坏已有生物膜方面均表现出优异的性能。值得注意的是，尽管ABP由于无法实现不可逆吸附而对抗噬菌体螃蟹Ab1513-BIM12没有效果，但ABP-Ce6对该菌株保持了有效的抗菌和生物膜消融作用，优于游离Ce6。这种持续的功效源于ABP的可逆吸附，它仍然可以使接近驱动的Ce6递送到目标细菌。在体内，ABP-Ce6显著促进了CRAB Ab1513和Ab1513- bim12引起的小鼠伤口愈合。综上所述，即使在细菌对噬菌体治疗产生耐药性后，ABP-Ce6作为治疗螃蟹感染的药物仍具有显著的疗效。这种新策略可能作为噬菌体治疗的一种有希望的补充策略，从而减少筛选新的治疗噬菌体的延迟。

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引用次数: 0

Wavelength-dependent excitation ratio of photosystem I and II in Arabidopsis thaliana 拟南芥光系统I和II的波长依赖性激发比

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-10-06 DOI: 10.1016/j.jphotobiol.2025.113278

Peter R. Bos, Herbert van Amerongen, Emilie Wientjes

Photosynthesis is a finely tuned process in which plants balance excitations between photosystem I (PSI) and photosystem II (PSII) to optimize energy conversion efficiency. The distribution of light energy between PSI and PSII across the full spectrum of photosynthetically active radiation is hard to quantify. Current attempts rely on estimating the PSI/PSII reaction center ratio and PS antenna sizes. In this study, we employed a streak-camera system to measure excitation distribution between PSI and PSII in Arabidopsis thaliana leaves over the wavelength range 430–630 nm, bypassing the need for indirect estimations of antenna size and pigment distribution. Our findings show that the absorption weighted PSII/(PSI + PSII) excitation ratio is 0.60 ± 0.01 in the 430–630 nm spectral range. By fitting the excitation spectrum with absorption spectra of PSI, PSII, and LHCII, we estimate the PSII/(PSI + PSII) reaction center ratio to be 0.58 ± 0.004. The excitation ratio shows that in the dark-adapted supercomplex organisation, PSII is overexcited. By recording a light response curve of ΦPSI and ΦPSII, we determined that the dark-adapted supercomplex organisation leads to overexcitation of PSII in low to medium light illumination intensities (∼372 μmol m⁻² s⁻¹). However, state transitions alone can rebalance the charge separation ratio. The quantitative excitation ratio and its correlation with reaction center ratios provide crucial parameters for refining photosynthetic models and understanding energy distribution across photosystems.

光合作用是植物平衡光系统I （PSI）和光系统II （PSII）之间的激发以优化能量转换效率的精细调节过程。在整个光合有效辐射光谱中，PSI和PSII之间的光能分布很难量化。目前的尝试依赖于估计PSI/PSII反应中心比和PS天线尺寸。在这项研究中，我们利用条纹相机系统测量了拟南芥叶片中PSI和PSII在430-630 nm波长范围内的激发分布，绕过了间接估计天线尺寸和色素分布的需要。结果表明，在430 ~ 630 nm光谱范围内，吸收加权PSII/(PSI + PSII)激发比为0.60±0.01。将激发光谱与PSI、PSII和LHCII的吸收光谱拟合，得到PSII/(PSI + PSII)反应中心比为0.58±0.004。激发比表明，在适应黑暗的超复杂组织中，PSII被过度激发。通过记录ΦPSI和ΦPSII的光响应曲线，我们确定了适应黑暗的超复杂组织导致PSII在低到中等光照强度（~ 372 μmol m−2 s−1）下过度激发。然而，状态跃迁本身可以重新平衡电荷分离比。定量激发比及其与反应中心比的相关性为完善光合模型和理解光系统间的能量分布提供了关键参数。

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引用次数: 0

Combined nanodiamond-mediated drug delivery and upconversion phototherapy for enhanced liver cancer treatment 纳米金刚石介导的药物传递和上转化光疗联合用于增强肝癌治疗

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-09-05 DOI: 10.1016/j.jphotobiol.2025.113260

Yun Teng , Zhige Li , Zheng Cui , Linfeng Wan , Zhuo Li , Xin Zhang , Lesheng Teng , Junsong Liu , Hongdong Li

To mitigate the significant side effects and limited efficacy of traditional treatment methods, we propose a novel strategy that involves the use of nanodiamond (ND) drug loading combined with up-converted blue light irradiation to achieve highly efficient chemotherapy-photodynamic treatment of liver tumor cells, specifically for the HepG2 cell line. ND-loaded drugs delivered a high concentration of doxorubicin to HepG2 cells, enhancing the chemotherapy effect. Upon exposure to near-infrared irradiation, blue light is excited by up-conversion nanoparticles (UCNPs), thereby activating the production of reactive oxygen demonstrated that the dual-mode therapy of chemotherapy and photodynamic therapy showed significantly improved anticancer effects. The inhibition rates were 77.4 % in vitro and 88.1 % in vivo for HepG2 cells, which are significantly higher than those treated with single photodynamic therapy (18.9 %, 28.6 %) or drug-loaded therapy (62.6 %, 71.4 %). It is noted that under the treatment conditions, no obvious tissue injury or inflammation was observed in the heart, liver, spleen, lung, and kidney samples collected from mice, indicating that the UCNPs and NDs exhibit good biocompatibility and are less toxic to normal cells and tissues, which would be favorable for clinical applications.

为了减轻传统治疗方法的显著副作用和有限的疗效，我们提出了一种新的策略，包括使用纳米金刚石（ND）药物负载结合上转换蓝光照射，以实现高效的化疗光动力治疗肝肿瘤细胞，特别是HepG2细胞系。nd负载药物向HepG2细胞递送高浓度的阿霉素，增强化疗效果。在近红外照射下，蓝光被上转换纳米粒子（UCNPs）激发，从而激活活性氧的产生，表明化疗和光动力双模式治疗显着提高了抗癌效果。体外和体内对HepG2细胞的抑制率分别为77.4%和88.1%，明显高于单次光动力治疗组（分别为18.9%和28.6%）和载药治疗组（分别为62.6%和71.4%）。在处理条件下，小鼠的心、肝、脾、肺、肾等组织均未出现明显的组织损伤和炎症反应，说明UCNPs和NDs具有良好的生物相容性，对正常细胞和组织的毒性较小，有利于临床应用。

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引用次数: 0

The integrated treatment of photodynamic therapy and oncolytic Virotherapy: A dual-modal paradigm for breast Cancer 光动力疗法和溶瘤病毒疗法的综合治疗：乳腺癌的双峰模式

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-08-28 DOI: 10.1016/j.jphotobiol.2025.113248

Yixiang Li , Kaidi Wang , Qianhao Min, Hongyu Lu, Guoqing Zhang, Jianhua Yan

Triple-negative breast cancer (TNBC) is a serious threat to lives and health. We developed a dual approach of Photodynamic therapy (PDT) and Newcastle Disease Oncolytic Virus (NDV) to mediate killing effects and anti-tumor immune effects against TNBC. In this study, we firstly verified that PDT combined with NDV effectively eliminated tumor cells. Cloning formation analysis, western blot and flow cytometer demonstrated that PDT + NDV triggered tumor cells ROS stress, apoptosis and inhibition of proliferation. Moreover, PDT + NDV upregulated the IL-6, STAT3 and NF-κB, increased the expression of HMGB1 and TNF-α, triggerd immunogenic cell death and strengthened the pro-inflammatory microenvironment, thereby effectively activating anti-tumor immune responses and enhancing the infiltration of CD8⁺ T cells. Our research demonstrated the synergistic anti-tumor effects of combining PDT and NDV, offering a promising strategy for the treatment of TNBC.

三阴性乳腺癌（TNBC）对生命和健康构成严重威胁。我们开发了光动力疗法（PDT）和新城病溶瘤病毒（NDV）的双重方法来介导对TNBC的杀伤作用和抗肿瘤免疫作用。在本研究中，我们首次验证了PDT联合NDV有效清除肿瘤细胞。克隆形态分析、western blot和流式细胞仪显示PDT + NDV可引发肿瘤细胞ROS应激、凋亡和增殖抑制。PDT + NDV上调IL-6、STAT3和NF-κB，增加HMGB1和TNF-α的表达，引发免疫原性细胞死亡，增强促炎微环境，从而有效激活抗肿瘤免疫应答，增强CD8+ T细胞浸润。我们的研究证明了PDT联合NDV的协同抗肿瘤作用，为TNBC的治疗提供了一个有希望的策略。

引用次数: 0

Restoration of carious dentin using diode irradiation on unmodified and chitosan nanoparticle-modified adhesive before LED polymerization: A SEM, FTIR, and μTBS evaluation. LED聚合前未改性和壳聚糖纳米颗粒改性胶粘剂二极管辐照修复牙本质龋病：SEM、FTIR和μTBS评价。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-09-16 DOI: 10.1016/j.jphotobiol.2025.113265

Amer M. Alanazi , Shaheryar Shafqat , Muhammad Omar Niaz , Zainab Dua

Aim

To investigate the restoration of caries-affected dentin (CAD) using adhesive modification technique via diode laser (DL) irradiation of unmodified and modified adhesive with Chitosan nanoparticles (CHNP) before light emitting diode (LED) polymerization, and CHNPs modified adhesive+LED polymerization without DL on micro-tensile bond strength (μTBS), Degree of conversion (DC) and resin tag length (RTL).

Material and methods

Forty-four human mandibular molars were obtained, and CAD was exposed according to ICDAS criteria 5. Teeth were positioned vertically within the auto-cure acrylic resin and randomly distributed into four categories according to the adhesive modification (n = 11). Group1-Etch(E) + Bond(B) + LED,Group 2-E + B(CHNP) + LED, Group 3-E + B + DL + LED and Group 4-E + B(CHNPS) + DL + LED. All samples were restored using composite buildup followed by thermal aging. Surface characterization of CHNP with Energy-dispersive spectroscopy (EDS) was assessed along with RTL via scanning electron microscopy (SEM). μTBS and failure modes were analyzed utilizing a universal testing machine (UTM) and stereomicroscope, respectively. The DC was quantified using a Fourier Transform Infrared Spectroscopy (FTIR). A one-way analysis of variance, complemented by Tukey's post hoc test, was implemented for comparisons among the investigated groups (p < 0.05).

Results

Group 4-E + B(CHNP) + DL + LED (107.43 ± 1.55 μm) presented the longest RTL with a score of grade 3 and the highest bond strength (11.54 ± 0.37 MPa). Whereas, Group 1 (E + B+ LED) displayed the shortest RTL (76.63 ± 0.82 μm) and lowest μTBS (9.31 ± 0.17 MPa). The adhesive irradiated with DL before LED polymerization in Groups 2 and 4 presented comparable DC (p˃0.05).

Conclusion

Pre-irradiating both unmodified and modified adhesives with chitosan nanoparticles using a diode laser before LED polymerization seems to be an effective approach. This technique enhances the material's mechanical properties by improving adhesive penetration, which in turn affects the length of resin tags,μTBS, and DC.

目的：通过二极管激光（DL）照射未改性和改性壳聚糖纳米颗粒（CHNP）的胶粘剂，观察未改性和改性壳聚糖纳米颗粒（CHNP）在发光二极管（LED）聚合前修复龋病牙本质（CAD），以及CHNP改性胶粘剂+LED无DL聚合的微拉伸粘结强度（μTBS）、转化度（DC）和树脂标签长度（RTL）的变化。材料与方法：取人下颌磨牙44颗，按照ICDAS标准进行CAD暴露5。将牙齿垂直放置在自动固化丙烯酸树脂中，根据粘接剂改性随机分为四类（n = 11）。Group1-Etch (E) +债券(B) +领导,集团双电子+ B (CHNP) +领导集团3 E + B + DL +领导和集团4 E + B (CHNPS) + DL +领导。所有样品都采用复合材料堆积，然后进行热老化。利用能量色散光谱（EDS）和扫描电子显微镜（SEM）对CHNP进行了表面表征。μTBS和失效模式分别采用通用试验机（UTM）和体视显微镜进行分析。利用傅里叶变换红外光谱（FTIR）对直流电进行定量分析。结果显示，4-E + B(CHNP) + DL + LED（107.43±1.55 μm）组的RTL最长，为3级，结合强度最高（11.54±0.37 MPa）。组1 （E + B+ LED）的RTL最短（76.63±0.82 μm）， μTBS最低（9.31±0.17 MPa）。2组和4组在LED聚合前用DL辐照后的DC值相当（p < 0.05）。结论：在LED聚合前，用二极管激光对未改性和改性的壳聚糖纳米颗粒进行预照射是一种有效的方法。该技术通过提高胶粘剂的渗透性来提高材料的机械性能，从而影响树脂标签、μTBS和DC的长度。

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引用次数: 0

Light-emitting diode-derived blue light overexposure accelerates corneal endothelial cell aging by inducing abnormal ROS accumulation 发光二极管来源的蓝光过度暴露通过诱导异常ROS积累加速角膜内皮细胞老化

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-09-01 DOI: 10.1016/j.jphotobiol.2025.113252

Zhaolin Liu , Yiran Yang , Ke Yan , Jieli Wu , Xiang Lin , Liying Tang , Qingjian Li , Caihong Huang , Jiaoyue Hu , Zuguo Liu

Blue light, defined as short-wavelength visible light ranging from 400 to 500 nm, is recognized for its high energy within the visible light spectrum. The prevalent use of light-emitting diodes (LEDs) has significantly increased exposure to blue light. Corneal endothelial cells (CECs) playing a crucial role in maintaining corneal transparency to get clear visual field. However, the specific effects of blue light on corneal endothelium remain unclear. To investigate this, in vivo and in vitro models of LED blue light irradiation were established. We examined changes in CEC fate and indicators related to oxidative stress. Our findings revealed that blue light exposure led to increased production of ROS in CECs, causing oxidative stress primarily in mitochondria. This, in turn, resulted in cell senescence, dysfunction, and apoptosis, ultimately contributing to the aging of corneal endothelium with accelerated cell loss. Notably, the rise in ROS levels triggered the activation of the Nrf2 signaling pathway in the early stages. This activation was associated with protective effects on CECs and inhibition of cell senescence. Our study sheds light on the intricate relationship between blue light exposure, oxidative stress, and the fate of CECs, providing valuable insights into the potential mechanisms underlying corneal aging.

蓝光被定义为波长在400至500纳米之间的短波可见光，因其在可见光光谱中的高能量而被认可。发光二极管（led）的普遍使用大大增加了蓝光的暴露。角膜内皮细胞在维持角膜透明度以获得清晰视野方面起着至关重要的作用。然而，蓝光对角膜内皮的具体影响尚不清楚。为此，我们建立了LED蓝光辐照的体内和体外模型。我们检查了CEC命运和氧化应激相关指标的变化。我们的研究结果表明，蓝光暴露导致CECs中ROS的产生增加，主要在线粒体中引起氧化应激。这反过来又导致细胞衰老、功能障碍和凋亡，最终导致角膜内皮老化，加速细胞损失。值得注意的是，ROS水平的升高在早期阶段触发了Nrf2信号通路的激活。这种激活与cec的保护作用和细胞衰老的抑制有关。我们的研究揭示了蓝光照射、氧化应激和CECs命运之间的复杂关系，为角膜衰老的潜在机制提供了有价值的见解。

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引用次数: 0

Neodymium-doped yttrium vanadate laser and Phthalocyanine photosensitizer doped chitosan nanoparticle activated via Photodynamic therapy on smear layer and push-out bond strength of fiber post: An in vitro SEM, EDX assessment 掺钕钒酸钇激光和酞菁光敏剂掺杂壳聚糖纳米粒子光动力活化对纤维桩涂抹层和推出粘结强度的影响：体外SEM， EDX评价

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-09-10 DOI: 10.1016/j.jphotobiol.2025.113263

Fahad Alkhudhairy

Aims

To quantify the effectiveness of different root canal sterilants, i.e., Sodium Hypochlorite (NaOCl), Neodymium-doped Yttrium Vanadate (Nd: YVO₄) laser, Phthalocyanine photosensitizer doped Chitosan nanoparticles activated via Photodynamic therapy (Pc-CNPs-PDT), on smear layer (SL) elimination and push out bond strength (PBS) of glass fiber posts (GFPs) bonded to root dentin.

Materials and methods

Forty-eight human premolars with an approximate root length measuring 15 ± 1 mm were included. Endodontic treatment was performed using a nickel‑titanium rotary instrument followed by obturation. The post space was prepared, and specimens were allocated to four groups based on the disinfection protocol employed. Group 1 (Saline + EDTA), Group 2 (2.25 % NaOCl + EDTA), Group 3 (Nd: YVO4 laser + EDTA), Group 4 (Pc-CNPs-PDT + EDTA). A scanning electron microscope was used to assess the surface topography of CHNPs, energy-dispersive spectroscopy (EDS), and SL removal efficacy. Each post was luted using a dual-cure resin cement. The bonded specimens were sectioned into slices (coronal, middle, and apical). A universal testing machine and a stereomicroscope were used to test the PBS and failure modes, respectively. The dataset underwent analysis through one-way analysis of variance ANOVA and Tukey's test to determine notable differences among groups (p = 0.05).

Results

Coronal section of Group 4 (Pc (CHNPs)-PDT + EDTA) samples exhibited maximum SL removal. Whereas, minimum SL elimination was displayed by Saline pretreated canals at the apical section. Samples irrigated with 2.5 % NaOCl + EDTA and Pc (CHNPs)-PDT + EDTA presented comparable scores of bond integrity at all three root sections.

Conclusion

Phthalocyanine photosensitizer doped Chitosan nanoparticles, activated via Photodynamic therapy, can be considered as a suitable alternative to the gold standard technique, NaOCl and EDTA, in removing smear layer and improving glass fiber post adhesion to canal dentin.

目的研究次氯酸钠（NaOCl）、掺钕钒酸钇（Nd: YVO4）激光、光动力疗法激活的酞青素光敏剂掺杂壳聚糖纳米颗粒（Pc-CNPs-PDT）等不同根管灭菌剂对玻璃纤维桩（gfp）黏结在根本质上的涂片层（SL）消除和推出粘结强度（PBS）的影响。材料与方法48颗人前磨牙，根长约为15±1 mm。采用镍钛旋转器械进行根管治疗，随后进行封闭。准备岗哨，按消毒方案将标本分为4组。1组（生理盐水+ EDTA）、2组（2.25% NaOCl + EDTA）、3组（Nd: YVO4激光+ EDTA）、4组（Pc-CNPs-PDT + EDTA）。采用扫描电镜对CHNPs的表面形貌、能谱分析（EDS）和SL去除效果进行了评价。每个桩使用双固化树脂水泥。将粘接标本切片（冠状、中、尖）。用万能试验机和体视显微镜分别对PBS和失效模式进行了测试。对数据集进行单因素方差分析和Tukey检验，各组间差异有统计学意义（p = 0.05）。结果第4组（Pc (CHNPs)-PDT + EDTA）样品的横切面显示出最大的SL去除。然而，经盐水预处理的根管在根尖部分显示出最小的SL消除。用2.5% NaOCl + EDTA和Pc (CHNPs)-PDT + EDTA灌溉的样品在所有三个根段的键完整性得分相当。结论经光动力活化的酞菁光敏剂掺杂壳聚糖纳米颗粒可作为金标准技术NaOCl和EDTA的替代材料，去除涂膜层，改善玻璃纤维桩与根管牙本质的粘附。

{"title":"Neodymium-doped yttrium vanadate laser and Phthalocyanine photosensitizer doped chitosan nanoparticle activated via Photodynamic therapy on smear layer and push-out bond strength of fiber post: An in vitro SEM, EDX assessment","authors":"Fahad Alkhudhairy","doi":"10.1016/j.jphotobiol.2025.113263","DOIUrl":"10.1016/j.jphotobiol.2025.113263","url":null,"abstract":"<div><h3>Aims</h3><div>To quantify the effectiveness of different root canal sterilants, i.e., Sodium Hypochlorite (NaOCl), Neodymium-doped Yttrium Vanadate (Nd: YVO<sub>4</sub>) laser, Phthalocyanine photosensitizer doped Chitosan nanoparticles activated via Photodynamic therapy (Pc-CNPs-PDT), on smear layer (SL) elimination and push out bond strength (PBS) of glass fiber posts (GFPs) bonded to root dentin.</div></div><div><h3>Materials and methods</h3><div>Forty-eight human premolars with an approximate root length measuring 15 ± 1 mm were included. Endodontic treatment was performed using a nickel‑titanium rotary instrument followed by obturation. The post space was prepared, and specimens were allocated to four groups based on the disinfection protocol employed. Group 1 (Saline + EDTA), Group 2 (2.25 % NaOCl + EDTA), Group 3 (Nd: YVO4 laser + EDTA), Group 4 (Pc-CNPs-PDT + EDTA). A scanning electron microscope was used to assess the surface topography of CHNPs, energy-dispersive spectroscopy (EDS), and SL removal efficacy. Each post was luted using a dual-cure resin cement. The bonded specimens were sectioned into slices (coronal, middle, and apical). A universal testing machine and a stereomicroscope were used to test the PBS and failure modes, respectively. The dataset underwent analysis through one-way analysis of variance ANOVA and Tukey's test to determine notable differences among groups (<em>p</em> = 0.05).</div></div><div><h3>Results</h3><div>Coronal section of Group 4 (Pc (CHNPs)-PDT + EDTA) samples exhibited maximum SL removal. Whereas, minimum SL elimination was displayed by Saline pretreated canals at the apical section. Samples irrigated with 2.5 % NaOCl + EDTA and Pc (CHNPs)-PDT + EDTA presented comparable scores of bond integrity at all three root sections.</div></div><div><h3>Conclusion</h3><div>Phthalocyanine photosensitizer doped Chitosan nanoparticles, activated via Photodynamic therapy, can be considered as a suitable alternative to the gold standard technique, NaOCl and EDTA, in removing smear layer and improving glass fiber post adhesion to canal dentin.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113263"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-IR quantum dot (BagQD) as fluorescent sensor for live-cell imaging and in-vivo antibacterial photocatalytic therapy (APT) 近红外量子点（BagQD）作为荧光传感器用于活细胞成像和体内抗菌光催化治疗（APT）

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-11-01 Epub Date: 2025-09-24 DOI: 10.1016/j.jphotobiol.2025.113271

Subhendu Sekhar Bag , Sayantan Sinha , Aniket Banerjee , Hemanshu Mediboyana , Animes K. Golder

The development of multitasking agents that can sense, as well as damage multidrug-resistant (MDR) bacteria with no growth, is the need of the hour to overcome the increased mortality associated with antibacterial resistance. Toward this end, to overcome the limitation of organic dyes as fluorescence probes, and other developed quantum dots (QD), we present new of its kind near infrared type-I (NIR-I) CdSe/ZnSe/FeTiO₃-based perovskite quantum dot, ^BagQD, for fluorescence-guided selective bacterial imaging-sensing and permanent disinfection. ^BagQD possesses unique photodynamic and photophysical properties of the near-infrared NIR-I biological window. The excellent electronic transitions of ^BagQD resulted in efficient quantitative fluorescent sensing-imaging of gram-positive (G+ve) as well as gram-negative (G-ve) bacterial pathogens, and complete in-situ and in-vivo disinfection/elimination of pathogenic bacteria without regrowth via damaging bacterial cell wall and cellular DNA, by ROS generation during photocatalysis. The material cytotoxicity study showed no harm to the human cell line. Moreover, the therapeutic application of ^BagQD on in-vivo mice skin wound infection, revealed the generation of hair follicles and epithelization within 6–8 days. Thus, ^BagQD serves as a new alternative to antibiotics and a smart nanomedicine that can uphold the hope in revolutionizing diagnostics and clinical avenues.

为了克服与抗菌药物耐药性相关的死亡率上升，迫切需要开发能够感知并破坏未生长的多药耐药（MDR）细菌的多任务制剂。为此，为了克服有机染料作为荧光探针和其他量子点（QD）的局限性，我们提出了一种新的近红外i型（NIR-I） CdSe/ZnSe/ fetio3基钙钛矿量子点BagQD，用于荧光引导的选择性细菌成像传感和永久消毒。BagQD具有独特的近红外NIR-I生物窗口光动力学和光物理特性。BagQD优异的电子跃迁特性使其能够对革兰氏阳性（G+ve）和革兰氏阴性（G-ve）病原菌进行高效的定量荧光传感成像，并在光催化过程中产生ROS，通过破坏细菌细胞壁和细胞DNA，完成病原菌的原位和体内消毒/消除，而不再生。细胞毒性实验表明，该物质对人体细胞系无危害。此外，BagQD对小鼠皮肤伤口感染的治疗作用显示，在6-8天内毛囊和上皮的产生。因此，BagQD作为抗生素的新替代品和智能纳米药物，可以为革命性的诊断和临床途径带来希望。

{"title":"Near-IR quantum dot (BagQD) as fluorescent sensor for live-cell imaging and in-vivo antibacterial photocatalytic therapy (APT)","authors":"Subhendu Sekhar Bag , Sayantan Sinha , Aniket Banerjee , Hemanshu Mediboyana , Animes K. Golder","doi":"10.1016/j.jphotobiol.2025.113271","DOIUrl":"10.1016/j.jphotobiol.2025.113271","url":null,"abstract":"<div><div>The development of multitasking agents that can sense, as well as damage multidrug-resistant (MDR) bacteria with no growth, is the need of the hour to overcome the increased mortality associated with antibacterial resistance. Toward this end, to overcome the limitation of organic dyes as fluorescence probes, and other developed quantum dots (QD), we present new of its kind near infrared type-I (NIR-I) CdSe/ZnSe/FeTiO<sub>3</sub>-based perovskite quantum dot, <sup><strong><em>Bag</em></strong></sup><strong><em>QD</em></strong>, for fluorescence-guided selective bacterial imaging-sensing and permanent disinfection. <sup><strong><em>Bag</em></strong></sup><strong><em>QD</em></strong> possesses unique photodynamic and photophysical properties of the near-infrared NIR-I biological window. The excellent electronic transitions of <sup><strong><em>Bag</em></strong></sup><strong><em>QD</em></strong> resulted in efficient quantitative fluorescent sensing-imaging of gram-positive (G+ve) as well as gram-negative (G-ve) bacterial pathogens, and complete in-situ and in-vivo disinfection/elimination of pathogenic bacteria without regrowth via damaging bacterial cell wall and cellular DNA, by ROS generation during photocatalysis. The material cytotoxicity study showed no harm to the human cell line. Moreover, the therapeutic application of <sup><strong><em>Bag</em></strong></sup><strong><em>QD</em></strong> on in-vivo mice skin wound infection, revealed the generation of hair follicles and epithelization within 6–8 days. Thus, <sup><strong><em>Bag</em></strong></sup><strong><em>QD</em></strong> serves as a new alternative to antibiotics and a smart nanomedicine that can uphold the hope in revolutionizing diagnostics and clinical avenues.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113271"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0