Journal of photochemistry and photobiology. B, Biology最新文献_第9页

Light-activated Zein–curcumin films for the photodynamic inactivation of bacterial biofilms: Continuous vs. intermittent illumination 用于细菌生物膜光动力失活的光活化玉米素-姜黄素薄膜：连续与间歇照明

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-19 DOI: 10.1016/j.jphotobiol.2025.113270

Gabriella Nunes Ferreira , Rafael Ravagnani da Costa , Guilherme A. Paixão , Jaison M. Júnior , Thaila Q. Corrêa , Antonio S.N. de Aguiar , Alessandra R. Lima , Vanderlei S. Bagnato , Sergio A. Yoshioka , Lucas D. Dias

The development of protein-based films derived from plants and agricultural sources has gained significant attention in the medical and food industries due to their broad applicability, sustainability, low cost, and non-toxic nature. Over the years, numerous studies have reported (photo)antimicrobial films aligned with the principles of the circular economy. In this study, zein-based films immobilized with curcumin as a photosensitizer (2.5 % and 5 % w/w) were synthesized and fully characterized using UV–Vis spectroscopy, fluorescence analysis, FT-IR, thermogravimetry (TG), and water contact angle measurements. Their potential for photodynamic applications was assessed through photobleaching analysis, confirming the generation of reactive oxygen species (ROS) at both concentrations. Furthermore, microbiological evaluations were conducted against Staphylococcus aureus biofilms under two different lighting regimes: continuous and intermittent exposure at specific time intervals (0, 6, 12, 18, and 24 h). The films demonstrated promising antimicrobial activity, achieving bacterial inactivation of up to 3.5 log reductions. These findings highlight the potential applications of zein-curcumin films as coatings for medical devices (e.g., endotracheal tubes, catheters) and as sustainable food packaging materials. The incorporation of photoantimicrobial properties into biodegradable films opens new perspectives for their use in healthcare and food preservation.

基于植物和农业来源的蛋白质薄膜的开发由于其广泛的适用性、可持续性、低成本和无毒性而在医疗和食品工业中受到了极大的关注。多年来，许多研究报告（图）抗菌膜符合循环经济的原则。本研究合成了以姜黄素为光敏剂（2.5%和5% w/w）固定的玉米蛋白基薄膜，并利用紫外可见光谱、荧光分析、红外光谱、热重（TG）和水接触角测量对其进行了全面表征。通过光漂白分析评估了它们的光动力学应用潜力，确认了两种浓度下活性氧（ROS）的产生。此外，在两种不同的光照条件下，对金黄色葡萄球菌生物膜进行了微生物学评估：在特定的时间间隔（0、6、12、18和24小时）连续照射和间歇照射。该薄膜表现出了良好的抗菌活性，实现了高达3.5 log的细菌灭活。这些发现突出了玉米素-姜黄素薄膜作为医疗器械涂层（例如，气管内管，导管）和可持续食品包装材料的潜在应用。将光抗菌特性纳入可生物降解薄膜为其在医疗保健和食品保存中的应用开辟了新的前景。

{"title":"Light-activated Zein–curcumin films for the photodynamic inactivation of bacterial biofilms: Continuous vs. intermittent illumination","authors":"Gabriella Nunes Ferreira , Rafael Ravagnani da Costa , Guilherme A. Paixão , Jaison M. Júnior , Thaila Q. Corrêa , Antonio S.N. de Aguiar , Alessandra R. Lima , Vanderlei S. Bagnato , Sergio A. Yoshioka , Lucas D. Dias","doi":"10.1016/j.jphotobiol.2025.113270","DOIUrl":"10.1016/j.jphotobiol.2025.113270","url":null,"abstract":"<div><div>The development of protein-based films derived from plants and agricultural sources has gained significant attention in the medical and food industries due to their broad applicability, sustainability, low cost, and non-toxic nature. Over the years, numerous studies have reported (photo)antimicrobial films aligned with the principles of the circular economy. In this study, zein-based films immobilized with curcumin as a photosensitizer (2.5 % and 5 % <em>w</em>/w) were synthesized and fully characterized using UV–Vis spectroscopy, fluorescence analysis, FT-IR, thermogravimetry (TG), and water contact angle measurements. Their potential for photodynamic applications was assessed through photobleaching analysis, confirming the generation of reactive oxygen species (ROS) at both concentrations. Furthermore, microbiological evaluations were conducted against <em>Staphylococcus aureus</em> biofilms under two different lighting regimes: continuous and intermittent exposure at specific time intervals (0, 6, 12, 18, and 24 h). The films demonstrated promising antimicrobial activity, achieving bacterial inactivation of up to 3.5 log reductions. These findings highlight the potential applications of zein-curcumin films as coatings for medical devices (e.g., endotracheal tubes, catheters) and as sustainable food packaging materials. The incorporation of photoantimicrobial properties into biodegradable films opens new perspectives for their use in healthcare and food preservation.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113270"},"PeriodicalIF":3.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular and tissue effects of photodynamic therapy in a murine cutaneous tumor model 光动力疗法对小鼠皮肤肿瘤模型血管和组织的影响

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-17 DOI: 10.1016/j.jphotobiol.2025.113269

Mirian Denise Stringasci , Michelle Barreto Requena , Clara Maria Gonçalves de Faria , Fernando Silva Ramalho , Vanderlei Salvador Bagnato

Skin cancer is the most common neoplasm worldwide and presents a significant health concern. Topical photodynamic therapy (PDT) has emerged as a therapeutic option for treating malignant and potentially precancerous skin lesions. To improve this technique and design more effective protocols, it is vital to understand the relationship between PDT damage in blood vessels and surrounding tissues. In this study, solid tumors of squamous cell carcinoma (SCC) were induced in balb/c nude mice. Damages were evaluated using histology and optical coherence tomography (OCT) images to compare PDT protocols using systemic and topical photosensitizer (PS) administration with different irradiances. The results are promising and may support the development of new and more effective PDT protocols for clinical treatment, including those that require multiple treatment sessions.

皮肤癌是世界上最常见的肿瘤，是一个重要的健康问题。局部光动力疗法（PDT）已成为治疗恶性和潜在癌前皮肤病变的一种治疗选择。为了改进这项技术并设计更有效的方案，了解血管和周围组织的PDT损伤之间的关系至关重要。本研究在balb/c裸鼠身上诱导鳞状细胞癌（SCC）实体瘤。使用组织学和光学相干断层扫描（OCT）图像对损伤进行评估，以比较使用不同辐照度的全身光敏剂和局部光敏剂（PS）的PDT方案。研究结果是有希望的，并可能支持开发新的和更有效的PDT临床治疗方案，包括那些需要多次治疗的方案。

引用次数: 0

Restoration of carious dentin using diode irradiation on unmodified and chitosan nanoparticle-modified adhesive before LED polymerization: A SEM, FTIR, and μTBS evaluation. LED聚合前未改性和壳聚糖纳米颗粒改性胶粘剂二极管辐照修复牙本质龋病：SEM、FTIR和μTBS评价。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-16 DOI: 10.1016/j.jphotobiol.2025.113265

Amer M. Alanazi , Shaheryar Shafqat , Muhammad Omar Niaz , Zainab Dua

Aim

To investigate the restoration of caries-affected dentin (CAD) using adhesive modification technique via diode laser (DL) irradiation of unmodified and modified adhesive with Chitosan nanoparticles (CHNP) before light emitting diode (LED) polymerization, and CHNPs modified adhesive+LED polymerization without DL on micro-tensile bond strength (μTBS), Degree of conversion (DC) and resin tag length (RTL).

Material and methods

Forty-four human mandibular molars were obtained, and CAD was exposed according to ICDAS criteria 5. Teeth were positioned vertically within the auto-cure acrylic resin and randomly distributed into four categories according to the adhesive modification (n = 11). Group1-Etch(E) + Bond(B) + LED,Group 2-E + B(CHNP) + LED, Group 3-E + B + DL + LED and Group 4-E + B(CHNPS) + DL + LED. All samples were restored using composite buildup followed by thermal aging. Surface characterization of CHNP with Energy-dispersive spectroscopy (EDS) was assessed along with RTL via scanning electron microscopy (SEM). μTBS and failure modes were analyzed utilizing a universal testing machine (UTM) and stereomicroscope, respectively. The DC was quantified using a Fourier Transform Infrared Spectroscopy (FTIR). A one-way analysis of variance, complemented by Tukey's post hoc test, was implemented for comparisons among the investigated groups (p < 0.05).

Results

Group 4-E + B(CHNP) + DL + LED (107.43 ± 1.55 μm) presented the longest RTL with a score of grade 3 and the highest bond strength (11.54 ± 0.37 MPa). Whereas, Group 1 (E + B+ LED) displayed the shortest RTL (76.63 ± 0.82 μm) and lowest μTBS (9.31 ± 0.17 MPa). The adhesive irradiated with DL before LED polymerization in Groups 2 and 4 presented comparable DC (p˃0.05).

Conclusion

Pre-irradiating both unmodified and modified adhesives with chitosan nanoparticles using a diode laser before LED polymerization seems to be an effective approach. This technique enhances the material's mechanical properties by improving adhesive penetration, which in turn affects the length of resin tags,μTBS, and DC.

目的：通过二极管激光（DL）照射未改性和改性壳聚糖纳米颗粒（CHNP）的胶粘剂，观察未改性和改性壳聚糖纳米颗粒（CHNP）在发光二极管（LED）聚合前修复龋病牙本质（CAD），以及CHNP改性胶粘剂+LED无DL聚合的微拉伸粘结强度（μTBS）、转化度（DC）和树脂标签长度（RTL）的变化。材料与方法：取人下颌磨牙44颗，按照ICDAS标准进行CAD暴露5。将牙齿垂直放置在自动固化丙烯酸树脂中，根据粘接剂改性随机分为四类（n = 11）。Group1-Etch (E) +债券(B) +领导,集团双电子+ B (CHNP) +领导集团3 E + B + DL +领导和集团4 E + B (CHNPS) + DL +领导。所有样品都采用复合材料堆积，然后进行热老化。利用能量色散光谱（EDS）和扫描电子显微镜（SEM）对CHNP进行了表面表征。μTBS和失效模式分别采用通用试验机（UTM）和体视显微镜进行分析。利用傅里叶变换红外光谱（FTIR）对直流电进行定量分析。结果显示，4-E + B(CHNP) + DL + LED（107.43±1.55 μm）组的RTL最长，为3级，结合强度最高（11.54±0.37 MPa）。组1 （E + B+ LED）的RTL最短（76.63±0.82 μm）， μTBS最低（9.31±0.17 MPa）。2组和4组在LED聚合前用DL辐照后的DC值相当（p < 0.05）。结论：在LED聚合前，用二极管激光对未改性和改性的壳聚糖纳米颗粒进行预照射是一种有效的方法。该技术通过提高胶粘剂的渗透性来提高材料的机械性能，从而影响树脂标签、μTBS和DC的长度。

{"title":"Restoration of carious dentin using diode irradiation on unmodified and chitosan nanoparticle-modified adhesive before LED polymerization: A SEM, FTIR, and μTBS evaluation.","authors":"Amer M. Alanazi , Shaheryar Shafqat , Muhammad Omar Niaz , Zainab Dua","doi":"10.1016/j.jphotobiol.2025.113265","DOIUrl":"10.1016/j.jphotobiol.2025.113265","url":null,"abstract":"<div><h3>Aim</h3><div>To investigate the restoration of caries-affected dentin (CAD) using adhesive modification technique via diode laser (DL) irradiation of unmodified and modified adhesive with Chitosan nanoparticles (CHNP) before light emitting diode (LED) polymerization, and CHNPs modified adhesive+LED polymerization without DL on micro-tensile bond strength (μTBS), Degree of conversion (DC) and resin tag length (RTL).</div></div><div><h3>Material and methods</h3><div>Forty-four human mandibular molars were obtained, and CAD was exposed according to ICDAS criteria 5. Teeth were positioned vertically within the auto-cure acrylic resin and randomly distributed into four categories according to the adhesive modification (<em>n</em> = 11). Group1-Etch(E) + Bond(B) + LED,Group 2-E + B(CHNP) + LED, Group 3-E + B + DL + LED and Group 4-E + B(CHNPS) + DL + LED. All samples were restored using composite buildup followed by thermal aging. Surface characterization of CHNP with Energy-dispersive spectroscopy (EDS) was assessed along with RTL via scanning electron microscopy (SEM). μTBS and failure modes were analyzed utilizing a universal testing machine (UTM) and stereomicroscope, respectively. The DC was quantified using a Fourier Transform Infrared Spectroscopy (FTIR). A one-way analysis of variance, complemented by Tukey's post hoc test, was implemented for comparisons among the investigated groups (<em>p</em> < 0.05).</div></div><div><h3>Results</h3><div>Group 4-E + B(CHNP) + DL + LED (<em>107.43 ± 1.55</em> μ<em>m) presented the longest RTL with a score of grade 3 and the highest bond strength</em> (<em>11.54 ± 0.37 MPa). Whereas,</em> Group 1 (E + B+ LED) displayed the shortest RTL (<em>76.63 ± 0.82</em> μ<em>m) and lowest</em> μTBS <em>(9.31</em> ± <em>0.17 MPa).</em> The adhesive irradiated with DL before LED polymerization in Groups 2 and 4 presented comparable DC (p˃0.05).</div></div><div><h3>Conclusion</h3><div>Pre-irradiating both unmodified and modified adhesives with chitosan nanoparticles using a diode laser before LED polymerization seems to be an effective approach. This technique enhances the material's mechanical properties by improving adhesive penetration, which in turn affects the length of resin tags,μTBS, and DC.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113265"},"PeriodicalIF":3.7,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of new chlorin e6 derivatives bearing galactose moieties on the macrocyclic periphery and their structure–activity relationships 含半乳糖大环的氯代e6衍生物的合成及其构效关系。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-16 DOI: 10.1016/j.jphotobiol.2025.113268

Marina V. Mal'shakova , Nikolay D. Belykh , Lydia N. Shestakova , Natalia E. Galochkina , Artem O. Belotelov , Sergey S. Soroko , Irina V. Balalaeva , Dmitry V. Belykh , Natalia Yu. Shilyagina

Recent studies of photosensitizers have increasingly focused on improving their aqueous solubility, reducing dark toxicity and enhancing photodynamic activity. In our work, we have synthesized and characterized a series of chlorin e₆ derivatives varying in the number of galactose moieties in the molecule and way they are linked to the chlorin macrocycle. We established that the quantity and localization of galactose moieties in the macrocycle affects both dark and photoinduced cytotoxicity. All of the studied derivatives exhibited singlet oxygen quantum yield photogeneration comparable to that of the clinically approved chlorin e₆ dimeglumine (Photoditazine®) with marginal exceeding. Although the incorporation of galactose moieties to the periphery of the chlorin macrocycle led to slightly decreased photostability of resulting derivatives, it did not significantly hinder their photodynamic activity. Moreover, cellular uptake of the obtained derivatives was significantly improved compared to that of Photoditazine®. Collectively, favorable spectral, photochemical, photobiological characteristics, along with improved aqueous solubility, suggest that chlorin e₆ derivatives with peripheral galactose substituents are promising photosensitizers for clinical applications.

近年来对光敏剂的研究越来越集中在改善其水溶性、降低暗毒性和光动力活性方面。在我们的工作中，我们合成并表征了一系列氯e6衍生物，这些衍生物在分子中半乳糖部分的数量和它们与氯大环的连接方式上都有所不同。我们确定了半乳糖部分在大环中的数量和定位影响暗和光诱导的细胞毒性。所有被研究的衍生物都表现出单线态氧量子产率，与临床批准的氯e6二甲基胺（photodiazine®）的产率相当，但边际超过。虽然半乳糖部分掺入到氯大环的外围会导致衍生物的光稳定性略有下降，但不会显著阻碍其光动力活性。此外，与Photoditazine®相比，所获得的衍生物的细胞摄取显着提高。总的来说，良好的光谱，光化学，光生物学特性，以及改善的水溶性，表明具有外周半乳糖取代基的氯e6衍生物是临床应用的有前途的光敏剂。

{"title":"Synthesis of new chlorin e6 derivatives bearing galactose moieties on the macrocyclic periphery and their structure–activity relationships","authors":"Marina V. Mal'shakova , Nikolay D. Belykh , Lydia N. Shestakova , Natalia E. Galochkina , Artem O. Belotelov , Sergey S. Soroko , Irina V. Balalaeva , Dmitry V. Belykh , Natalia Yu. Shilyagina","doi":"10.1016/j.jphotobiol.2025.113268","DOIUrl":"10.1016/j.jphotobiol.2025.113268","url":null,"abstract":"<div><div>Recent studies of photosensitizers have increasingly focused on improving their aqueous solubility, reducing dark toxicity and enhancing photodynamic activity. In our work, we have synthesized and characterized a series of chlorin e<sub>6</sub> derivatives varying in the number of galactose moieties in the molecule and way they are linked to the chlorin macrocycle. We established that the quantity and localization of galactose moieties in the macrocycle affects both dark and photoinduced cytotoxicity. All of the studied derivatives exhibited singlet oxygen quantum yield photogeneration comparable to that of the clinically approved chlorin e<sub>6</sub> dimeglumine (Photoditazine®) with marginal exceeding. Although the incorporation of galactose moieties to the periphery of the chlorin macrocycle led to slightly decreased photostability of resulting derivatives, it did not significantly hinder their photodynamic activity. Moreover, cellular uptake of the obtained derivatives was significantly improved compared to that of Photoditazine®. Collectively, favorable spectral, photochemical, photobiological characteristics, along with improved aqueous solubility, suggest that chlorin e<sub>6</sub> derivatives with peripheral galactose substituents are promising photosensitizers for clinical applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113268"},"PeriodicalIF":3.7,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual near-infrared wavelength photobiomodulation accelerates transdermal burn wound repair via anti-inflammatory, pain relief and redox-regulating mechanisms 双近红外波长光生物调节通过抗炎、缓解疼痛和氧化还原调节机制加速透皮烧伤创面修复。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-16 DOI: 10.1016/j.jphotobiol.2025.113267

Ashok Priyadarshi, Gaurav K. Keshri, Divya Yadav, Asheesh Gupta

Burns cause severe tissue damage and impose a significant psychological and financial burden, highlighting the need for innovative, effective, and cost-efficient therapeutic interventions for tissue repair. Research indicates that multimodal therapy enhances healing by positively regulating various phases of tissue repair. Photobiomodulation therapy (PBMT), which employs low-power light, offers a promising non-pharmacological energy-based healing approach for non-healing wounds. Absorbed photons stimulate cellular metabolism, increase ATP production, and modulate molecular signaling pathways, thereby facilitating the repair process. In this study, we investigated the effects of combined NIR pulsed 810 nm and superpulsed 904 nm lasers PBMT on inflammation, pain, and redox status during transdermal burn wound healing in rats. Animals were divided into five groups, namely uninjured, control (burn), pulsed 810 nm, superpulsed 904 nm lasers, and dual wavelength PBMT. Combined PBMT exhibited a positive synergistic effect on the burn repair process by significantly (P < 0.05) reducing inflammation (NF-κB, TNF-α, IL-1β, NOS-2, IL-6), pain (substance P-receptor, COX-2), and oxidative stress (ROS, 4-HNE adduct, LPO, 3-NT), while increasing redox potential/endogenous antioxidant (Nrf2, catalase), and maintaining cytoprotection (GRP78) seven days post-wounding. These molecular findings were well-supported by histopathological analysis, which indicated decreased mast cell infiltration, exhibiting the potent anti-inflammatory effects of the combined PBMT. Altogether, the current findings demonstrate that dual PBMT synergistically accelerates the repair of full-thickness burns, exhibits analgesic, anti-inflammatory responses, reduces oxidative stress, activates the antioxidant defense pathway, and maintains cellular redox homeostasis. This non-invasive dual NIR PBMT presents a promising translational therapeutic approach for burn repair in clinical care.

烧伤会造成严重的组织损伤，并造成严重的心理和经济负担，因此需要创新、有效和经济的组织修复治疗干预措施。研究表明，多模式治疗通过积极调节组织修复的各个阶段来促进愈合。光生物调节疗法（PBMT）采用低功率光，为非药物能量治疗非愈合性伤口提供了一种很有前途的方法。吸收的光子刺激细胞代谢，增加ATP的产生，调节分子信号通路，从而促进修复过程。在这项研究中，我们研究了近红外脉冲810 nm和超脉冲904 nm激光联合PBMT对大鼠透皮烧伤创面愈合过程中炎症、疼痛和氧化还原状态的影响。实验动物分为5组，分别为未损伤组、对照组（烧伤组）、810 nm脉冲激光组、超脉冲904 nm激光组和双波长PBMT组。联合PBMT在烧伤修复过程中表现出积极的协同作用

{"title":"Dual near-infrared wavelength photobiomodulation accelerates transdermal burn wound repair via anti-inflammatory, pain relief and redox-regulating mechanisms","authors":"Ashok Priyadarshi, Gaurav K. Keshri, Divya Yadav, Asheesh Gupta","doi":"10.1016/j.jphotobiol.2025.113267","DOIUrl":"10.1016/j.jphotobiol.2025.113267","url":null,"abstract":"<div><div>Burns cause severe tissue damage and impose a significant psychological and financial burden, highlighting the need for innovative, effective, and cost-efficient therapeutic interventions for tissue repair. Research indicates that multimodal therapy enhances healing by positively regulating various phases of tissue repair. Photobiomodulation therapy (PBMT), which employs low-power light, offers a promising non-pharmacological energy-based healing approach for non-healing wounds. Absorbed photons stimulate cellular metabolism, increase ATP production, and modulate molecular signaling pathways, thereby facilitating the repair process. In this study, we investigated the effects of combined NIR pulsed 810 nm and superpulsed 904 nm lasers PBMT on inflammation, pain, and redox status during transdermal burn wound healing in rats. Animals were divided into five groups, namely uninjured, control (burn), pulsed 810 nm, superpulsed 904 nm lasers, and dual wavelength PBMT. Combined PBMT exhibited a positive synergistic effect on the burn repair process by significantly (<em>P</em> < 0.05) reducing inflammation (NF-κB, TNF-α, IL-1β, NOS-2, IL-6), pain (substance P-receptor, COX-2), and oxidative stress (ROS, 4-HNE adduct, LPO, 3-NT), while increasing redox potential/endogenous antioxidant (Nrf2, catalase), and maintaining cytoprotection (GRP78) seven days post-wounding. These molecular findings were well-supported by histopathological analysis, which indicated decreased mast cell infiltration, exhibiting the potent anti-inflammatory effects of the combined PBMT. Altogether, the current findings demonstrate that dual PBMT synergistically accelerates the repair of full-thickness burns, exhibits analgesic, anti-inflammatory responses, reduces oxidative stress, activates the antioxidant defense pathway, and maintains cellular redox homeostasis. This non-invasive dual NIR PBMT presents a promising translational therapeutic approach for burn repair in clinical care.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113267"},"PeriodicalIF":3.7,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ sonoporation to enhance the tumour uptake of silicon phthalocyanine and improve PDT effectiveness in a triple negative breast cancer murine model 原位超声穿孔增强肿瘤对硅酞菁的摄取并改善三阴性乳腺癌小鼠模型的PDT效果。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-12 DOI: 10.1016/j.jphotobiol.2025.113266

Martina Capozza , Alberto Mangia , Michela Gagliardi , Rachele Stefania , Francesca Garello , Laura Conti , Enzo Terreno

The effectiveness of photodynamic therapy (PDT) has been well demonstrated in vitro, but in vivo studies have only shown a delay in tumour growth. Tumour recurrence is often reported in clinical trials and is usually associated with limited tumour uptake of the photosensitiser (PS). In this study, sonoporation (SNP) is utilised as a physical targeting tool to enhance the uptake of an untargeted PS in cells and tumour tissues. Using a chemometric approach, we identified the optimal sonoporation stimulus to maximise uptake and cell viability (frequency 1.05 MHz, tON (percentage of positive signal in a single pulse): 50 %, DC: 90 %, burst: 1 s, sonoporation time 1 min). We achieved a viability of 83.9 % ± 10.3, and an uptake in live cells of 53.1 % ± 6.6. The combination of sonoporation and photodynamic therapy resulted in a significant reduction in cell viability. In a triple-negative breast cancer model, sonoporation combined with photodynamic therapy significantly inhibited tumour growth. For the first time, our results highlight the potential of sonoporation as a non-invasive method to increase the intratumour uptake of photosensitisers, offering a promising strategy to improve PDT efficacy.

光动力疗法（PDT）的有效性已经在体外得到了很好的证明，但在体内的研究只显示了肿瘤生长的延迟。肿瘤复发经常在临床试验中报道，通常与有限的肿瘤摄取光敏剂（PS）有关。在本研究中，sonoporization （SNP）被用作物理靶向工具，以增强细胞和肿瘤组织中非靶向PS的摄取。使用化学测量方法，我们确定了最佳的声穿孔刺激，以最大限度地提高摄取和细胞活力（频率1.05 MHz, tON（单脉冲中阳性信号的百分比）：50%,DC: 90%，爆发：1秒，声穿孔时间1分钟）。我们获得了83.9%±10.3%的活力，53.1%±6.6的活细胞摄取。声波穿孔和光动力疗法的结合导致细胞活力显著降低。在三阴性乳腺癌模型中，超声穿孔联合光动力疗法显著抑制肿瘤生长。我们的研究结果首次强调了超声穿孔作为一种非侵入性方法增加肿瘤内光敏剂摄取的潜力，为提高PDT疗效提供了一种有希望的策略。

{"title":"In situ sonoporation to enhance the tumour uptake of silicon phthalocyanine and improve PDT effectiveness in a triple negative breast cancer murine model","authors":"Martina Capozza , Alberto Mangia , Michela Gagliardi , Rachele Stefania , Francesca Garello , Laura Conti , Enzo Terreno","doi":"10.1016/j.jphotobiol.2025.113266","DOIUrl":"10.1016/j.jphotobiol.2025.113266","url":null,"abstract":"<div><div>The effectiveness of photodynamic therapy (PDT) has been well demonstrated <em>in vitro</em>, but <em>in vivo</em> studies have only shown a delay in tumour growth. Tumour recurrence is often reported in clinical trials and is usually associated with limited tumour uptake of the photosensitiser (PS). In this study, sonoporation (SNP) is utilised as a physical targeting tool to enhance the uptake of an untargeted PS in cells and tumour tissues. Using a chemometric approach, we identified the optimal sonoporation stimulus to maximise uptake and cell viability (frequency 1.05 MHz, tON (percentage of positive signal in a single pulse): 50 %, DC: 90 %, burst: 1 s, sonoporation time 1 min). We achieved a viability of 83.9 % ± 10.3, and an uptake in live cells of 53.1 % ± 6.6. The combination of sonoporation and photodynamic therapy resulted in a significant reduction in cell viability. In a triple-negative breast cancer model, sonoporation combined with photodynamic therapy significantly inhibited tumour growth. For the first time, our results highlight the potential of sonoporation as a non-invasive method to increase the intratumour uptake of photosensitisers, offering a promising strategy to improve PDT efficacy.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113266"},"PeriodicalIF":3.7,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated laser speckle contrast and multispectral imaging for in vivo multimodal assessment of small intestinal viability 集成激光散斑对比和多光谱成像用于小肠活力的体内多模式评估。

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-11 DOI: 10.1016/j.jphotobiol.2025.113264

Yi Xie , Danfei Huang , Jiaxuan Yan , Libin Zhu , Tianci Weng , Lechao Zhang , Zhonghao Xie , Guangzao Huang , Shujat Ali , Xiaojing Chen

Accurate intraoperative identification of necrotic intestinal segments remains challenging owing to the limitations of invasive methods or single-parameter techniques. A multimodal system integrating laser speckle contrast and multispectral imaging was developed. The noninvasive system simultaneously acquires images of blood flow index (BFI) and tissue oxygen saturation (StO₂), which enables the assessment of tissue metabolic rate of oxygen (tMRO₂). For in vivo calculation of StO₂ in the small intestine, a linear transformation algorithm based on three hemoglobin isosbestic points was employed, and an optimal characteristic wavelength (763 nm) was selected to reduce background interference. The system was validated in the small intestinal ischemia and reperfusion model of rabbits. During occlusion, BFI and tMRO₂ rapidly declined by approximately 80 %, while StO₂ gradually decreased, ultimately by approximately 46 %. Upon reperfusion, segments with reversible damage exhibited a significant recovery. In contrast, irreversibly necrotic regions showed only transient increases before secondary decline. Although either modality alone can identify necrotic intestinal segments that are indiscernible to the human eye, the location of occluded mesenteric vessels cannot be accurately identified. The multimodal imaging approach resolved this limitation, and enabled reliable identification of potentially and irreversibly necrotic segments, as confirmed by pathological analysis. This system provides a noninvasive and cost-effective solution for intraoperative intestinal viability assessment and surgical precision.

由于侵入性方法或单参数技术的局限性，术中准确识别坏死肠段仍然具有挑战性。研制了一种集激光散斑对比和多光谱成像于一体的多模态系统。该无创系统同时获取血流指数（BFI）和组织氧饱和度（StO2）图像，从而能够评估组织氧代谢率（tMRO2）。在体内计算小肠StO2时，采用基于3个血红蛋白等吸点的线性变换算法，选择最佳特征波长（763 nm）以减少背景干扰。该系统在家兔小肠缺血再灌注模型中得到验证。遮挡期间，BFI和tMRO2迅速下降约80%，而StO2逐渐下降，最终下降约46%。再灌注时，可逆性损伤的节段表现出明显的恢复。相反，不可逆坏死区域在继发性下降之前仅显示短暂的增加。虽然单独的两种方式都可以识别人眼无法识别的坏死肠段，但闭塞的肠系膜血管的位置不能准确识别。多模态成像方法解决了这一限制，并能够可靠地识别潜在的和不可逆的坏死节段，病理分析证实了这一点。该系统为术中肠道活力评估和手术精度提供了一种无创且经济高效的解决方案。

{"title":"Integrated laser speckle contrast and multispectral imaging for in vivo multimodal assessment of small intestinal viability","authors":"Yi Xie , Danfei Huang , Jiaxuan Yan , Libin Zhu , Tianci Weng , Lechao Zhang , Zhonghao Xie , Guangzao Huang , Shujat Ali , Xiaojing Chen","doi":"10.1016/j.jphotobiol.2025.113264","DOIUrl":"10.1016/j.jphotobiol.2025.113264","url":null,"abstract":"<div><div>Accurate intraoperative identification of necrotic intestinal segments remains challenging owing to the limitations of invasive methods or single-parameter techniques. A multimodal system integrating laser speckle contrast and multispectral imaging was developed. The noninvasive system simultaneously acquires images of blood flow index (BFI) and tissue oxygen saturation (StO<sub>2</sub>), which enables the assessment of tissue metabolic rate of oxygen (tMRO<sub>2</sub>). For in vivo calculation of StO<sub>2</sub> in the small intestine, a linear transformation algorithm based on three hemoglobin isosbestic points was employed, and an optimal characteristic wavelength (763 nm) was selected to reduce background interference. The system was validated in the small intestinal ischemia and reperfusion model of rabbits. During occlusion, BFI and tMRO<sub>2</sub> rapidly declined by approximately 80 %, while StO<sub>2</sub> gradually decreased, ultimately by approximately 46 %. Upon reperfusion, segments with reversible damage exhibited a significant recovery. In contrast, irreversibly necrotic regions showed only transient increases before secondary decline. Although either modality alone can identify necrotic intestinal segments that are indiscernible to the human eye, the location of occluded mesenteric vessels cannot be accurately identified. The multimodal imaging approach resolved this limitation, and enabled reliable identification of potentially and irreversibly necrotic segments, as confirmed by pathological analysis. This system provides a noninvasive and cost-effective solution for intraoperative intestinal viability assessment and surgical precision.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113264"},"PeriodicalIF":3.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neodymium-doped yttrium vanadate laser and Phthalocyanine photosensitizer doped chitosan nanoparticle activated via Photodynamic therapy on smear layer and push-out bond strength of fiber post: An in vitro SEM, EDX assessment 掺钕钒酸钇激光和酞菁光敏剂掺杂壳聚糖纳米粒子光动力活化对纤维桩涂抹层和推出粘结强度的影响：体外SEM， EDX评价

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-10 DOI: 10.1016/j.jphotobiol.2025.113263

Fahad Alkhudhairy

Aims

To quantify the effectiveness of different root canal sterilants, i.e., Sodium Hypochlorite (NaOCl), Neodymium-doped Yttrium Vanadate (Nd: YVO₄) laser, Phthalocyanine photosensitizer doped Chitosan nanoparticles activated via Photodynamic therapy (Pc-CNPs-PDT), on smear layer (SL) elimination and push out bond strength (PBS) of glass fiber posts (GFPs) bonded to root dentin.

Materials and methods

Forty-eight human premolars with an approximate root length measuring 15 ± 1 mm were included. Endodontic treatment was performed using a nickel‑titanium rotary instrument followed by obturation. The post space was prepared, and specimens were allocated to four groups based on the disinfection protocol employed. Group 1 (Saline + EDTA), Group 2 (2.25 % NaOCl + EDTA), Group 3 (Nd: YVO4 laser + EDTA), Group 4 (Pc-CNPs-PDT + EDTA). A scanning electron microscope was used to assess the surface topography of CHNPs, energy-dispersive spectroscopy (EDS), and SL removal efficacy. Each post was luted using a dual-cure resin cement. The bonded specimens were sectioned into slices (coronal, middle, and apical). A universal testing machine and a stereomicroscope were used to test the PBS and failure modes, respectively. The dataset underwent analysis through one-way analysis of variance ANOVA and Tukey's test to determine notable differences among groups (p = 0.05).

Results

Coronal section of Group 4 (Pc (CHNPs)-PDT + EDTA) samples exhibited maximum SL removal. Whereas, minimum SL elimination was displayed by Saline pretreated canals at the apical section. Samples irrigated with 2.5 % NaOCl + EDTA and Pc (CHNPs)-PDT + EDTA presented comparable scores of bond integrity at all three root sections.

Conclusion

Phthalocyanine photosensitizer doped Chitosan nanoparticles, activated via Photodynamic therapy, can be considered as a suitable alternative to the gold standard technique, NaOCl and EDTA, in removing smear layer and improving glass fiber post adhesion to canal dentin.

目的研究次氯酸钠（NaOCl）、掺钕钒酸钇（Nd: YVO4）激光、光动力疗法激活的酞青素光敏剂掺杂壳聚糖纳米颗粒（Pc-CNPs-PDT）等不同根管灭菌剂对玻璃纤维桩（gfp）黏结在根本质上的涂片层（SL）消除和推出粘结强度（PBS）的影响。材料与方法48颗人前磨牙，根长约为15±1 mm。采用镍钛旋转器械进行根管治疗，随后进行封闭。准备岗哨，按消毒方案将标本分为4组。1组（生理盐水+ EDTA）、2组（2.25% NaOCl + EDTA）、3组（Nd: YVO4激光+ EDTA）、4组（Pc-CNPs-PDT + EDTA）。采用扫描电镜对CHNPs的表面形貌、能谱分析（EDS）和SL去除效果进行了评价。每个桩使用双固化树脂水泥。将粘接标本切片（冠状、中、尖）。用万能试验机和体视显微镜分别对PBS和失效模式进行了测试。对数据集进行单因素方差分析和Tukey检验，各组间差异有统计学意义（p = 0.05）。结果第4组（Pc (CHNPs)-PDT + EDTA）样品的横切面显示出最大的SL去除。然而，经盐水预处理的根管在根尖部分显示出最小的SL消除。用2.5% NaOCl + EDTA和Pc (CHNPs)-PDT + EDTA灌溉的样品在所有三个根段的键完整性得分相当。结论经光动力活化的酞菁光敏剂掺杂壳聚糖纳米颗粒可作为金标准技术NaOCl和EDTA的替代材料，去除涂膜层，改善玻璃纤维桩与根管牙本质的粘附。

{"title":"Neodymium-doped yttrium vanadate laser and Phthalocyanine photosensitizer doped chitosan nanoparticle activated via Photodynamic therapy on smear layer and push-out bond strength of fiber post: An in vitro SEM, EDX assessment","authors":"Fahad Alkhudhairy","doi":"10.1016/j.jphotobiol.2025.113263","DOIUrl":"10.1016/j.jphotobiol.2025.113263","url":null,"abstract":"<div><h3>Aims</h3><div>To quantify the effectiveness of different root canal sterilants, i.e., Sodium Hypochlorite (NaOCl), Neodymium-doped Yttrium Vanadate (Nd: YVO<sub>4</sub>) laser, Phthalocyanine photosensitizer doped Chitosan nanoparticles activated via Photodynamic therapy (Pc-CNPs-PDT), on smear layer (SL) elimination and push out bond strength (PBS) of glass fiber posts (GFPs) bonded to root dentin.</div></div><div><h3>Materials and methods</h3><div>Forty-eight human premolars with an approximate root length measuring 15 ± 1 mm were included. Endodontic treatment was performed using a nickel‑titanium rotary instrument followed by obturation. The post space was prepared, and specimens were allocated to four groups based on the disinfection protocol employed. Group 1 (Saline + EDTA), Group 2 (2.25 % NaOCl + EDTA), Group 3 (Nd: YVO4 laser + EDTA), Group 4 (Pc-CNPs-PDT + EDTA). A scanning electron microscope was used to assess the surface topography of CHNPs, energy-dispersive spectroscopy (EDS), and SL removal efficacy. Each post was luted using a dual-cure resin cement. The bonded specimens were sectioned into slices (coronal, middle, and apical). A universal testing machine and a stereomicroscope were used to test the PBS and failure modes, respectively. The dataset underwent analysis through one-way analysis of variance ANOVA and Tukey's test to determine notable differences among groups (<em>p</em> = 0.05).</div></div><div><h3>Results</h3><div>Coronal section of Group 4 (Pc (CHNPs)-PDT + EDTA) samples exhibited maximum SL removal. Whereas, minimum SL elimination was displayed by Saline pretreated canals at the apical section. Samples irrigated with 2.5 % NaOCl + EDTA and Pc (CHNPs)-PDT + EDTA presented comparable scores of bond integrity at all three root sections.</div></div><div><h3>Conclusion</h3><div>Phthalocyanine photosensitizer doped Chitosan nanoparticles, activated via Photodynamic therapy, can be considered as a suitable alternative to the gold standard technique, NaOCl and EDTA, in removing smear layer and improving glass fiber post adhesion to canal dentin.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113263"},"PeriodicalIF":3.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined nanodiamond-mediated drug delivery and upconversion phototherapy for enhanced liver cancer treatment 纳米金刚石介导的药物传递和上转化光疗联合用于增强肝癌治疗

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-05 DOI: 10.1016/j.jphotobiol.2025.113260

Yun Teng , Zhige Li , Zheng Cui , Linfeng Wan , Zhuo Li , Xin Zhang , Lesheng Teng , Junsong Liu , Hongdong Li

To mitigate the significant side effects and limited efficacy of traditional treatment methods, we propose a novel strategy that involves the use of nanodiamond (ND) drug loading combined with up-converted blue light irradiation to achieve highly efficient chemotherapy-photodynamic treatment of liver tumor cells, specifically for the HepG2 cell line. ND-loaded drugs delivered a high concentration of doxorubicin to HepG2 cells, enhancing the chemotherapy effect. Upon exposure to near-infrared irradiation, blue light is excited by up-conversion nanoparticles (UCNPs), thereby activating the production of reactive oxygen demonstrated that the dual-mode therapy of chemotherapy and photodynamic therapy showed significantly improved anticancer effects. The inhibition rates were 77.4 % in vitro and 88.1 % in vivo for HepG2 cells, which are significantly higher than those treated with single photodynamic therapy (18.9 %, 28.6 %) or drug-loaded therapy (62.6 %, 71.4 %). It is noted that under the treatment conditions, no obvious tissue injury or inflammation was observed in the heart, liver, spleen, lung, and kidney samples collected from mice, indicating that the UCNPs and NDs exhibit good biocompatibility and are less toxic to normal cells and tissues, which would be favorable for clinical applications.

为了减轻传统治疗方法的显著副作用和有限的疗效，我们提出了一种新的策略，包括使用纳米金刚石（ND）药物负载结合上转换蓝光照射，以实现高效的化疗光动力治疗肝肿瘤细胞，特别是HepG2细胞系。nd负载药物向HepG2细胞递送高浓度的阿霉素，增强化疗效果。在近红外照射下，蓝光被上转换纳米粒子（UCNPs）激发，从而激活活性氧的产生，表明化疗和光动力双模式治疗显着提高了抗癌效果。体外和体内对HepG2细胞的抑制率分别为77.4%和88.1%，明显高于单次光动力治疗组（分别为18.9%和28.6%）和载药治疗组（分别为62.6%和71.4%）。在处理条件下，小鼠的心、肝、脾、肺、肾等组织均未出现明显的组织损伤和炎症反应，说明UCNPs和NDs具有良好的生物相容性，对正常细胞和组织的毒性较小，有利于临床应用。

{"title":"Combined nanodiamond-mediated drug delivery and upconversion phototherapy for enhanced liver cancer treatment","authors":"Yun Teng , Zhige Li , Zheng Cui , Linfeng Wan , Zhuo Li , Xin Zhang , Lesheng Teng , Junsong Liu , Hongdong Li","doi":"10.1016/j.jphotobiol.2025.113260","DOIUrl":"10.1016/j.jphotobiol.2025.113260","url":null,"abstract":"<div><div>To mitigate the significant side effects and limited efficacy of traditional treatment methods, we propose a novel strategy that involves the use of nanodiamond (ND) drug loading combined with up-converted blue light irradiation to achieve highly efficient chemotherapy-photodynamic treatment of liver tumor cells, specifically for the HepG2 cell line. ND-loaded drugs delivered a high concentration of doxorubicin to HepG2 cells, enhancing the chemotherapy effect. Upon exposure to near-infrared irradiation, blue light is excited by up-conversion nanoparticles (UCNPs), thereby activating the production of reactive oxygen demonstrated that the dual-mode therapy of chemotherapy and photodynamic therapy showed significantly improved anticancer effects. The inhibition rates were 77.4 % in vitro and 88.1 % in vivo for HepG2 cells, which are significantly higher than those treated with single photodynamic therapy (18.9 %, 28.6 %) or drug-loaded therapy (62.6 %, 71.4 %). It is noted that under the treatment conditions, no obvious tissue injury or inflammation was observed in the heart, liver, spleen, lung, and kidney samples collected from mice, indicating that the UCNPs and NDs exhibit good biocompatibility and are less toxic to normal cells and tissues, which would be favorable for clinical applications.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113260"},"PeriodicalIF":3.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin safety of 233 nm far UV-C ex vivo and in vivo – Pilot study for evaluating different populations and multiple exposures 233nm远紫外- c的体外和体内皮肤安全性——评估不同人群和多次暴露的初步研究

IF 3.7 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of photochemistry and photobiology. B, Biology

Pub Date : 2025-09-05 DOI: 10.1016/j.jphotobiol.2025.113262

Daniela F. Zamudio Díaz , Constanze Baber , Holger Klose , Jan Ruschel , Sascha Rohn , Martina C. Meinke , Johannes Schleusener

Nosocomial infections remain a major healthcare challenge, underlining the demand for antimicrobial technologies. Far UV-C (200–235 nm) has emerged as a safer alternative to traditional 254 nm UV-C for microbial reduction on skin and wounds but also in occupied spaces due to its strong germicidal properties and minimal skin penetration. However, studies on humans remain limited.

This study aimed at evaluating the skin safety of 233 nm UV-C for potential applications in antisepsis and public area decontamination. Ex vivo experiments first assessed age-dependent DNA damage before proceeding to in vivo studies. Healthy volunteers of varying skin types and ages underwent single exposures to evaluate the effects of age and pigmentation. Additionally, young and light-skinned volunteers received multiple exposures. Biopsies were collected to assess DNA damage and repair.

A biocidal dose of 233 nm induced superficial DNA damage, showing lower damage than that induced by suberythemal UV-B exposure, a recognized safe dose for human skin. Older and dark-skinned participants exhibited higher residual DNA damage 24 h-post-exposure compared to younger and lighter-skinned individuals, though nearly complete repair was observed after seven days. Repeated exposures, up to cumulative doses of 120 mJ/cm² and 240 mJ/cm², did not induce significant changes on skin pigmentation, antioxidant activity, or immune response. However, DNA damage accumulation suggested limitations in repair mechanisms over 24 h. These results confirm the safety of a single exposure to 233 nm, but highlight potential risks with repeated applications, emphasizing the need for careful dose regulation and further in vivo studies, including well-established treatments at 222 nm.

医院感染仍然是一个主要的卫生保健挑战，强调了对抗菌技术的需求。远紫外线- c （200-235 nm）已成为传统254 nm紫外线- c更安全的替代品，用于皮肤和伤口上的微生物减少，但由于其强大的杀菌性能和最小的皮肤穿透性，也适用于占用空间。然而，对人类的研究仍然有限。本研究旨在评估233nm UV-C在防腐和公共场所去污方面的潜在应用。在进行体内研究之前，体外实验首先评估了年龄依赖性DNA损伤。不同皮肤类型和年龄的健康志愿者接受了单次暴露，以评估年龄和色素沉着的影响。此外，年轻和浅肤色的志愿者接受了多次接触。收集活检来评估DNA损伤和修复。233nm的生物杀灭剂剂量可诱导浅表DNA损伤，其损伤程度低于暴露于红斑下UV-B（公认的人体皮肤安全剂量）所造成的损伤。与年轻和皮肤较浅的人相比，年龄较大和皮肤黝黑的参与者在暴露24小时后表现出更高的残留DNA损伤，尽管在7天后观察到几乎完全修复。重复暴露，累积剂量达到120 mJ/cm2和240 mJ/cm2，不会引起皮肤色素沉着、抗氧化活性或免疫反应的显著变化。然而，DNA损伤积累表明修复机制在24小时内的局限性。这些结果证实了单次暴露于233 nm的安全性，但强调了重复应用的潜在风险，强调需要仔细调节剂量和进一步的体内研究，包括222 nm的成熟治疗。

{"title":"Skin safety of 233 nm far UV-C ex vivo and in vivo – Pilot study for evaluating different populations and multiple exposures","authors":"Daniela F. Zamudio Díaz , Constanze Baber , Holger Klose , Jan Ruschel , Sascha Rohn , Martina C. Meinke , Johannes Schleusener","doi":"10.1016/j.jphotobiol.2025.113262","DOIUrl":"10.1016/j.jphotobiol.2025.113262","url":null,"abstract":"<div><div>Nosocomial infections remain a major healthcare challenge, underlining the demand for antimicrobial technologies. Far UV-C (200–235 nm) has emerged as a safer alternative to traditional 254 nm UV-C for microbial reduction on skin and wounds but also in occupied spaces due to its strong germicidal properties and minimal skin penetration. However, studies on humans remain limited.</div><div>This study aimed at evaluating the skin safety of 233 nm UV-C for potential applications in antisepsis and public area decontamination. Ex vivo experiments first assessed age-dependent DNA damage before proceeding to in vivo studies. Healthy volunteers of varying skin types and ages underwent single exposures to evaluate the effects of age and pigmentation. Additionally, young and light-skinned volunteers received multiple exposures. Biopsies were collected to assess DNA damage and repair.</div><div>A biocidal dose of 233 nm induced superficial DNA damage, showing lower damage than that induced by suberythemal UV-B exposure, a recognized safe dose for human skin. Older and dark-skinned participants exhibited higher residual DNA damage 24 h-post-exposure compared to younger and lighter-skinned individuals, though nearly complete repair was observed after seven days. Repeated exposures, up to cumulative doses of 120 mJ/cm<sup>2</sup> and 240 mJ/cm<sup>2</sup>, did not induce significant changes on skin pigmentation, antioxidant activity, or immune response. However, DNA damage accumulation suggested limitations in repair mechanisms over 24 h. These results confirm the safety of a single exposure to 233 nm, but highlight potential risks with repeated applications, emphasizing the need for careful dose regulation and further in vivo studies, including well-established treatments at 222 nm.</div></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"272 ","pages":"Article 113262"},"PeriodicalIF":3.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0