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Eco-friendly Management of Fungal Wilt of Tomato Caused by Fusarium oxysporum f. sp. Lycopersici 番茄尖孢镰刀菌枯萎病的生态管理
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.12.229
G. Biswal, D. Singh
The trial was conducted at Central Farm, Orissa University of Agriculture and Technology, Bhubaneswar in two consecutive years to study the efficacy of bio-agents in comparison with the fungicide, antibiotic and chemicals to manage wilt disease caused by Fusarium oxysporum f. sp. lycopersici. In the present study, two potent bio-agents Trichoderma viride (107 spore/ml)and Pseudomonas fluorescens (6 × 108cfu/ml)obtained from city market were also used for root dip of seedlings of tomato cv.BT-10 and soil treatment individually and also in combined form. The experimental results revealed that minimum wilt (2.89%) incidence and maximum yield (74.16 q/ha) was recorded from the treatment where seedling root dip with Carbendazim @ 0.15% and Streptocycline 0.015% + soil drench with Carbendazim (0.2%), Plantomycin (0.1%) and Blitox-50 (0.3%) was practised. It was followed by seedling root dip and soil treatment by only P. fluorescens which produced (70.35 q/ha) and was also better than other treatments in coastal plains of Odisha. Therefore, it is advisable to go for P. fluorescens as seedling dip and soil treatment against theFusarium wilt in tomato in coastal plains of Odisha.
该试验连续两年在布巴内斯瓦尔奥里萨农业技术大学的中央农场进行,目的是研究生物制剂与杀菌剂、抗生素和化学药品在防治由番茄枯萎病引起的枯萎病方面的效果。本研究还利用从城市市场获得的两种强效生物制剂——绿木霉(107孢子/ml)和荧光假单胞菌(6 × 108cfu/ml)对番茄幼苗进行了根浸。BT-10和土壤单独或联合处理。结果表明,多菌灵(0.15%)根浸+链霉素(0.015%)+多菌灵(0.2%)、植物霉素(0.1%)和百立思-50(0.3%)土壤浸水处理的青枯病发生率最低(2.89%),产量最高(74.16 q/ha)。在奥里萨邦沿海平原,幼苗根浸处理次之,土壤单施荧光藻处理产量为70.35 q/ha,也优于其他处理。因此,在奥里萨邦沿海平原,番茄枯萎病防治宜采用荧光假单胞菌作为苗剂和土壤处理。
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引用次数: 1
Effects of Mesorhizobium ciceri and Biochar on the Growth, Nodulation and Antifungal Activity Against Root Pathogenic Fungi in Chickpea (Cicer arietinum L.) 环孢中根瘤菌和生物炭对鹰嘴豆生长、结瘤及抗根病原菌活性的影响
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.520
M. Jahan, U. Shahzad, S. A. Naqvi, I. Tahir, T. Abbas, M. Iqbal, Phoebe Nemenzo
This study was conducted to investigate the effects of the inoculation of Mesorhizobium ciceri on the nodulation, growth and antagonistic expression against soil-borne fungal pathogens (Phytophthora medicaginis, Fusarium oxysporum and Fusarium solani) on Cicer arietinum L. or commonly known as chickpea grown in vermiculite medium amended with 5% green waste (GW) biochar. The combination of M. ciceri and biochar showed significant effect to chickpea compared with other treatments and control plants in terms of nodulation. The chickpea inoculated with M. ciceri and amended with biochar produced the highest nodule number with an average value of 110 nodules per plant and with an average nodule fresh weight of 57.90 mg per plant at 60 days harvest. The other treatments (M. ciceri only and biochar only) and the positive control (2 mM nitrate-treated plants) produced an average of 55, 65 and 15 nodules per plant with the corresponding average nodule weight of 39.5, 46.5 and 35.6 mg per plant, respectively, 60 days after seed sowing. The combined M. ciceri and biochar also enhanced the shoot length, and fresh and dry weights of chickpea. However, it was observed that the primary root length was shorter than the control but clusters of feeder roots were observed. The combination of M. ciceri and biochar also completely inhibited the colony development of all root pathogenic fungi of chickpea after three days of inoculation. Therefore, the inoculation of M. ciceri in vermiculite medium amended with green waste biochar enhanced the nodulation and growth conditions in chickpea as well as inhibited the growth of root pathogenic fungi P. medicaginis, F. oxysporum and F. solani.
本试验研究了在蛭石培养基中接种环孢中根瘤菌对鹰嘴豆(Cicer arietinum L.,俗称鹰嘴豆)结瘤、生长和对土源真菌病原菌(疫霉、尖孢镰刀菌和枯萎菌)拮抗表达的影响。与其他处理和对照植物相比,绿僵菌与生物炭组合处理对鹰嘴豆结瘤效果显著。在收获60 d时,接种西芹霉菌并加生物炭改性的鹰嘴豆的单株平均结瘤数最高,达到110个,单株平均结瘤鲜重为57.90 mg。播种后60 d,其他处理(单处理和单处理生物炭)和阳性对照(2 mM硝酸盐处理)平均每株产生55、65和15个根瘤,相应的平均根瘤重分别为39.5、46.5和35.6 mg /株。与生物炭复合处理也能提高鹰嘴豆的茎长、鲜重和干重。然而,观察到主根长度比对照短,但观察到成群的取食根。接种3天后,绿僵菌与生物炭的组合也完全抑制了鹰嘴豆所有根致病真菌的菌落发育。由此可见,在添加了绿色废生物炭的蛭石培养基中接种青霉菌,改善了鹰嘴豆的结瘤和生长条件,抑制了鹰嘴豆根部病原菌P. medicaginis、F. oxysporum和F. solani的生长。
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引用次数: 2
Potential Role of Native Arbuscular Mycorrhizal Fungi (AMF) in theRestoration of Laurisilva 乡土丛枝菌根真菌(AMF)在月桂树恢复中的潜在作用
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.503
Catarina Drumonde-Melo, P. Borges, H. Freitas, Luis V. Nunes
The beneficial association of seedlings with arbuscular mycorrhizal fungi (AMF) is thought to improve early tree establishment through increased uptake of poorly labile soil nutrients (particularly P) and enhancing plant tolerance to biotic and abiotic factors. Seedlings of Juniperus brevifolia, an endemic woody plant of the Azores archipelago with potential commercial value, was grown in the nursery with and without inoculation by a commercial plant growth promoter consisting of AMF isolated from the Azores (MICOazorica). Treatments were arranged in a randomized complete block design in a greenhouse. At six months after planting, all AMF-inoculated plants were colonized. The percentage of colonization varied between 46% and 96% (Mean 70%). At harvest, all physical parameters were significantly greater in AMF-inoculated plants relative to uninoculated plants. Based on the obtained results, we strongly advise the use of native AMF, in strategies used in restoration programs in the Azores.
幼苗与丛枝菌根真菌(AMF)的有益联系被认为通过增加对不稳定土壤养分(特别是磷)的吸收和增强植物对生物和非生物因素的耐受性来改善早期树木的建立。摘要采用从亚速尔群岛分离的AMF组成的商业植物生长促进剂,在苗圃中接种和不接种短叶刺柏(Juniperus brevifolia)幼苗。在温室中按随机完全区组设计进行处理。种植6个月后,所有接种amf的植株均定植。定植率在46% ~ 96%之间(平均70%)。收获时,接种amf植株的所有物理参数均显著高于未接种植株。根据获得的结果,我们强烈建议在亚速尔群岛的恢复计划中使用本地AMF。
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引用次数: 0
Editorial Highlights for Journal of Plant Pathology and Microbiology 植物病理学与微生物学杂志社论要闻
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.E496
S. Kumar
I am pleased to introduce Journal of Plant Pathology & Microbiology a rapid peer reviewed Journal which have a key concerns over the plant pathology practices by exploring the best pathological and plant related microbiological research and by exhibiting this information both directly, as pathological findings, and in practice oriented formats of direct application in day-to-day situations. I am pleased to announce that, all issues of volume 11 were published online well within the time and the print issues were also brought out and dispatched within 30 days of publishing the issue online during the year of 2019.
我很高兴向大家介绍《植物病理学与微生物学杂志》,这是一本经过快速同行评审的杂志,它通过探索最好的病理学和植物相关微生物学研究,并通过直接展示这些信息,作为病理发现,以及在日常情况下直接应用于实践导向的形式,来关注植物病理学实践。我很高兴地宣布,2019年第11卷的所有问题都在网上顺利出版,印刷问题也在网上出版后30天内提出并发出。
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引用次数: 0
Biochemical Studies in Rust Resistant and Susceptible Genotypes of Soybean 大豆抗锈病和敏感基因型的生化研究
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.505
K. Sachin, Nargund Vb, J. Shamarao
Biochemical parameters play an important role in resistance or susceptibility in plants against diseases. Development of resistant varieties is the most appropriate approach to control the disease and the concept is now developing to explore the built-in plant defense mechanism in relation to pathogen attack. An attempt was made to study the biochemical factors imparting rust resistance in soybean genotypes at 75 days after sowing (DAS). The resistant genotypes had more of total phenols than susceptible genotypes. Total sugar (Reducing and Non reducing) content was more in resistant genotypes compared to susceptible genotypes at 75 DAS. The resistant genotypes EC- 241780 recorded maximum (5.78 mg/g.) reducing sugar followed by 4.75 mg/g in EC- 241778 (P) genotype and least was recorded 3.31 mg/g in JS-335 susceptible genotype. Among the genotypes EC 241778 recorded highest non reducing sugar content of 2.27 mg g of fresh wt followed by DSb 21 (2.25 mg/g). The least non-reducing sugar content of 1.55 mg/g was recorded in JS 335 followed by JS 93-05, (1.63 mg/g).
生物化学参数在植物抗病或易感性中起着重要作用。培育抗病品种是控制病害最合适的途径,目前这一概念正在发展,以探索与病原体攻击有关的植物内在防御机制。对播后75 d大豆基因型抗锈病的生化因素进行了研究。抗性基因型的总酚含量高于敏感基因型。在75 DAS时,抗性基因型的总糖(还原性和非还原性)含量高于敏感基因型。抗性基因型EC- 241780的还原糖含量最高(5.78 mg/g),其次是EC- 241778 (P)基因型的4.75 mg/g,而敏感基因型JS-335的还原糖含量最低(3.31 mg/g)。在基因型中,EC 241778的非还原糖含量最高,为2.27 mg/g,其次是dsb21 (2.25 mg/g)。非还原糖含量最低的品种是JS 335,为1.55 mg/g,其次是JS 93-05,为1.63 mg/g。
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引用次数: 0
A First Record of Exserohilum rostratum as a New Pathogen Causing Bean Blight in Egypt. 在埃及首次发现引起大豆枯萎病的新病原菌。
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.496
Farag Mf, Attia Fm
Seedling blight of bean (Phaseolus vulgaris L.) was recorded in bean fields at five different localities in Beni Sweif Governorate, Egypt. Symptoms appeared as green dark to purplish-brown spots, with brown margins. The affected plant leaves were collected for mycological analysis. Percentage of disease incidence were 30%, 25%, 22%, 15% and 35% in El-Wasta, Nasser, Beni Sweif, Sumosta and Beba counties respectively. Leaf samples were surface sterilized and cultured on potato dextrose agar. The growing fungi were identified on morphological as well as on molecular basis. Microscopic examination revealed that the isolated organisms have the same characteristics of Exserohilum rostratum (Drechsler) Leonard & Suggs. Among the 30 fungal isolates collected from the five bean plantations, a representative isolate was grown for DNA extraction, PCR and rDNA sequencing. Universal primers targeting ITS regions of the rDNA were used for PCR and sequencing. Results confirmed that the sequences of these fungi showed close relationship with E. rostratum with 99.6% - 100% similarity. The obtained sequences were deposited in the GenBank with accession numbers MT075801, MT071830, MT071831, MT071832, and MT071834. Pathogenicity tests confirmed that E. rostratum strains were pathogenic showing the same disease symptoms previously observed on bean plants in the study areas. The minimum temperature for spore germination was 5°C, the optimum temperature was 35°C, and the maximum temperature was 50°C. On the other hand, conidial germination was stopped at both lower (3°C) and higher (55°C) temperatures. Studying the host range of the fungus showed that the pathogen was able to attack tomato, pepper, squash and potato beside common bean and watermelon.
在埃及贝尼·斯韦弗省5个不同地区的豆田中记录了大豆幼苗枯萎病。症状表现为绿、暗至紫褐色斑点,边缘为棕色。采集受感染植物叶片进行真菌学分析。El-Wasta、Nasser、Beni Sweif、Sumosta和Beba县的发病率分别为30%、25%、22%、15%和35%。对叶片样品进行表面灭菌,并在马铃薯葡萄糖琼脂上培养。对生长真菌进行了形态和分子鉴定。显微镜检查显示,分离的生物体具有与rostratum exserhilum (Drechsler) Leonard & Suggs相同的特征。从5个大豆种植园收集的30株真菌分离株中,培养有代表性的分离株进行DNA提取、PCR和rDNA测序。使用针对rDNA ITS区域的通用引物进行PCR和测序。结果表明,这些真菌序列与rostratum亲缘关系密切,相似度为99.6% ~ 100%。获得的序列存储在GenBank中,登录号为MT075801、MT071830、MT071831、MT071832和MT071834。致病性试验证实,该菌株具有致病性,表现出与研究区豆科植物相同的疾病症状。孢子萌发的最低温度为5℃,最适温度为35℃,最高温度为50℃。另一方面,在较低(3℃)和较高(55℃)的温度下,分生孢子的萌发都停止了。对病原菌的寄主范围研究表明,除普通豆和西瓜外,该病菌还能侵染番茄、辣椒、南瓜和马铃薯。
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引用次数: 4
Field Evaluation of Bread Wheat (Triticum aestivum L.) Genotypes for Stripe Rust (Puccinia striiformis W.) Resistance in Arsi Highlands of Oromia Region, South -Eastern-Ethiopia 小麦(Triticum aestivum L.)田间评价条锈病(Puccinia striiformis W.)基因型埃塞俄比亚东南部奥罗米亚地区阿尔西高地的抵抗
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.521
Getnet Muche Abebele, Merkuz Abera Admasu, Bekele Hundie Agdu
Wheat is one of the world's most important crops whose grain production is increasing year after year. However, the emerged virulent stripe rust races at one point of the world spread to the rest of wheat producing countries by wind as well as human travels and damaged popular resistant wheat cultivars thereby posed food insecurity. This study was carried out with the aim to identify possible sources of stripe rust resistance among Ethiopian bread wheat pipelines for durable resistance breeding. Twenty-eight advanced bread wheat pipelines, local susceptible and resistant check cultivars Kubsa and Wane respectively were field tested in randomized complete block design with three replications across two stripe rust hot-spot locations for their slow rusting characteristics. Slow rusting resistance at the adultplant stage was assessed through the determination of final rust severity (FRS), average coefficient of infection (ACI), and relative area under disease progressive curve (rAUDPC). Among the twenty-eight, 24, 2 and 2 genotypes displayed high, moderate and low level of slow rusting over two locations respectively. The results revealed that wheat lines, ETBW- 8858, ETBW-8870, ETBW-8583, ETBW-8668, ETBW-8595, ETBW-8684, ETBW-9548, ETBW-9549, ETBW-9552, ETBW-9554, ETBW-9558, ETBW-9559, ETBW-9560, ETBW-875, ETBW-8802, ETBW-8862, ETBW- 8804, ETBW-8896, ETBW-9556, ETBW-9557, ETBW-8991,ETBW-9486,ETBW-9556 and ETBW-9561 had low values of FRS, ACI and rAUDPC and were regarded as good slow rusting lines. Strong positive correlations were observed between different parameters of slow rusting. As compared with susceptible, resistant check variety and other test lines, three lines namely. ETBW-8684; ETBW-9558 and ETBW-8751 are high yielders and could be released for production. Twenty-four lines with high and moderate levels of slow rusting and expected to possess both major and minor resistance genes could be used for durable stripe rust resistance breeding in wheat. However, is advised to postulate inherent resistance genes in these lines and their seedling susceptibility for providing fruitful recommendations.
小麦是世界上最重要的作物之一,其粮食产量逐年增加。然而,在世界某个地方出现的有毒条锈病通过风和人类旅行传播到其他小麦生产国,并破坏了流行的抗性小麦品种,从而造成了粮食不安全。本研究的目的是确定埃塞俄比亚面包小麦管道中可能的条锈病抗性来源,以便进行持久抗性育种。以28个高级面包小麦管道为材料,分别采用3个随机完全区组设计,在2个条锈病热点地区对其缓锈特性进行田间试验。通过测定最终锈病严重程度(FRS)、平均侵染系数(ACI)和疾病进展曲线下相对面积(rAUDPC)来评价成虫期的慢锈病抗性。28个基因型中,24个基因型、2个基因型和2个基因型分别在2个地点表现出高、中、低慢锈程度。结果表明,ETBW- 8858、ETBW-8870、ETBW-8583、ETBW-8668、ETBW-8595、ETBW-8684、ETBW-9548、ETBW-9549、ETBW-9552、ETBW-9558、ETBW-9559、ETBW-9560、ETBW-875、ETBW-8802、ETBW- 8804、ETBW-8896、ETBW-9556、ETBW-9557、ETBW-8991、ETBW-9486、ETBW-9556和ETBW-9561等小麦品系的FRS、ACI和rAUDPC值较低,属于缓蚀优良品系。慢锈各参数之间存在较强的正相关关系。与易感、耐检品种和其他试验品系相比,即三种品系。etbw - 8684;ETBW-9558和ETBW-8751产量高,可以投放生产。24个缓锈程度高和中等、预计同时具有主、次要抗性基因的品系可用于小麦持久条锈病抗性选育。然而,建议假设这些品种的内在抗性基因及其幼苗敏感性,以便提供富有成效的建议。
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引用次数: 2
Evaluation of Bioactive Compounds of Some Common and Traditional MedicinalPlants in Relevance with the Growth of Paddy 与水稻生长有关的几种常用和传统药用植物生物活性成分的评价
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.511
B. Sridevi, S. Lokesh
India, in terms of its natural resources, is a very rich country since thousands of medicinal plants are found growing and can as well be accessed for their benefits. Manufacturing drugs using plant products has been a field of utmost importance these days. The therapeutic value behind such plants can be exposed to the world only if it is brought out in a consumable form. Assessing the phytochemistry of each plant before determining its medicinal property and the condition that it can target is crucial. Several phytochemical tests and bioassays are therefore necessary to evaluate the various chemical compounds in the plants. In this study, weeds viz., Leucas aspera (Lamiaceae), Tridax procumbens (Asteraceae), Justiciaadhathoda (Acanthaceae), Alternanthera sessilis (Amaranthaceae), Phyllanthus niruri (Euphorbiaceae), Acalypha indica (Euphorbiaceae) and six medicinal plants Rauvolfia tetraphylla (Apocynaceae), Achyranthes aspera (Amaranthaceae), Tinospora cordifolia (Menispermaceae), Bacopa monnieri (Scrophulariaceae), Eclipta prostrata (Asteraceae) and Clitoria ternatea (Fabaceae) were chosen to investigate their phytochemical composition, phenolic content, flavonoid content, anti-fungal activity and their effect on paddy seed germination. Extraction was carried out using methanol. The highest phenolic content was observed in extract of P. niruri (29.66mg/g GAE). In contrast Leucas aspera showed highest flavonoid content (12.76mg/g QAE). P. niruri at its higher concentration indicated the reduced incidence of fungi like Alternaria padwickii, Verticillium cinnabarinum and Drechslera oryzae which was from 9 to 2%, 5 to 2%, and 10 to 3%, respectively. These findings indicated the importance of common traditional plants in agriculture apart from their medicinal value.
就其自然资源而言,印度是一个非常丰富的国家,因为发现有成千上万的药用植物在生长,并且可以获得它们的益处。利用植物产品制造药物已成为当今最重要的领域。这些植物背后的治疗价值只有以可消费的形式出现,才能暴露给世界。在确定每种植物的药用特性和可靶向的条件之前,评估每种植物的植物化学是至关重要的。因此,有必要进行几种植物化学试验和生物测定,以评估植物中的各种化合物。本研究选取了六种药用植物,分别为:细叶草(Lamiaceae)、三叶草(Asteraceae)、刺叶草(Justiciaadhathoda)、苋属(amaranaceae)、千叶草(Phyllanthus niruri)、刺叶草(Acalypha indica)和四叶草(Apocynaceae)、牛膝草(amaranaceae)、堇叶草(menispermacae)、马齿苋(rophulariaceae)、马齿苋(Bacopa monnieri)。以菊科赤藓(Eclipta prostrata)和豆科阴蒂(Clitoria ternatea)为研究对象,研究了其植物化学成分、酚类含量、类黄酮含量、抗真菌活性及其对水稻种子萌发的影响。用甲醇进行萃取。其中,乌桕提取物中酚类物质含量最高,为29.66mg/g GAE。黄酮类化合物的含量最高,为12.76mg/g QAE。P. niruri浓度越高,padwickii Alternaria、朱砂黄萎病菌(Verticillium cinnabarinum)和稻瘟病菌(Drechslera oryzae)的发病率分别从9%降至2%、5%降至2%和10%降至3%。这些发现表明,除了药用价值外,常见的传统植物在农业中的重要性。
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引用次数: 0
Studies on Predominant Epiphytic Micro-flora as Antagonists to Postharvest Pathogens of Apple 苹果采后病原菌拮抗优势附生菌群的研究
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.512
Aqleema Banoo, Efath Shahnaz, B. Saba, Ay, R. S. Rasool, Taibah Bashir, R. Latif
Apple (Malus domestica Borkh) is an important horticultural crop that is affected by the number of diseases round the year. The fruit is particularly susceptible to a number of pathogens both pre- and post-harvest. Management of these diseases is based mostly on the application of synthetic fungicides with obvious disadvantages of environmental pollution, health hazards, pathogen resistance, etc. In the present study, eleven epiphytes were isolated using potato dextrose agar, nutrient agar and yeast maltose agar media. Amongst them, five fungal isolates viz., Aspergillus sp. (I1), Penicillium sp. (I2), Fusarium sp. (I3), Rhizopus sp. (I4) and Alternaria sp. (I5) and six bacterial isolates viz., Pseudomonas sp. (I6), Pseudomonas sp. (I7), Bacillus sp. (I8), Bacillus sp. (I9), Staphylococcus sp. (I10) and Micrococcus sp. (I11) were predominantly noticed under all the three methods (leaf impression, serial dilution and fruit washing) and were hence, used for further studies. The highest average colony count of 3.62 colonies/cm2 was recorded in fruit washing method followed by leaf impression (3.17) and lowest in serial dilution method (2.12). The in vitro screening of various bacterial and fungal epiphytes revealed that isolates of Pseudomonas sp. (I6) and Bacillus (I8 and I9) were the only bacterial strains capable of inhibiting the growth of all the test pathogens using dual culture method. Assays on wounded apples revealed that Pseudomonas sp. I6 at 107 cfu/ml was effective antagonist against Penicillium sp. and Fusarium sp., Bacillus sp. I9 at 107 cfu/ml was effective antagonist against Alternaria sp., whereas, Bacillus sp. I8 at 107 cfu/ml was most effective antagonist against Diplodia sp. The present study revealed that the antagonists were more or less efficient towards each pathogen and can be utilized for the management of post-harvest diseases of apple.
苹果(Malus domestica Borkh)是一种重要的园艺作物,一年四季都受病害的影响。水果在收获前和收获后都特别容易受到许多病原体的影响。这些病害的治理多以应用合成杀菌剂为主,但其环境污染、危害健康、病原体耐药等缺点明显。本研究采用马铃薯葡萄糖琼脂、营养琼脂和酵母麦芽糖琼脂培养基分离了11株附生植物。其中,曲霉(I1)、青霉(I2)、镰刀菌(I3)、根霉(I4)、互菌(I5)等5株真菌和假单胞菌(I6)、假单胞菌(I7)、芽孢杆菌(I8)、芽孢杆菌(I9)、葡萄球菌(I10)、微球菌(I11)等6株细菌在叶片压印、连续稀释、洗果等3种方法下均表现突出,值得进一步研究。平均菌落数最高的是洗果法,为3.62个/cm2,其次是叶片压印法(3.17个),最低的是连续稀释法(2.12个)。多种细菌和真菌附生菌的体外筛选表明,采用双重培养法,只有假单胞菌(Pseudomonas sp., I6)和芽孢杆菌(Bacillus, I8和I9)能抑制所有试验病原菌的生长。在伤苹果上的试验表明,107 cfu/ml的假单胞菌sp. I6对青霉菌和镰刀菌有效,107 cfu/ml的芽孢杆菌sp. I9对交替菌有效,107 cfu/ml的芽孢杆菌sp. I8对双plodia sp.最有效。本研究表明,这些拮抗剂对每一种病原菌都有或多或少的拮抗效果,可用于苹果采后病害的防治。
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引用次数: 2
Efficacy of Essential oil of Two Melaleuca Species in the Treatment of Infectious Diseases 两种千层树精油治疗感染性疾病的疗效观察
Pub Date : 2020-01-01 DOI: 10.35248/2157-7471.20.11.516
Aqsa Qurban, A. Ameen, H. Ishfaq
Purpose of this study was to evaluate antibacterial activity of oil extract obtained from parts (leaves) of Melaleuca species against bacterial strains to treat infectious diseases such as Urinary Tract Infection. In general, extracts obtained by extraction method showed antibacterial activity against different tested microorganisms. Agar well diffusion method was used to evaluate antibacterial activity against ten pathogens which include 7 different strains of E. coli, 2 strains of Klebsiella pneumoni and 1 strain of Entercoccus faecalis. According to the results of this study, Essential oils of Melaleuca plant showed the good antibacterial action of the bacterial strains due to the measurement of clear zone of inhibition but antibiotic susceptibility assay was more appreciable. Antibiotics are used as positive control and ethanol as negative control along with it. In conclusion, Extracts of Melaleuca species found to be containing chemical compounds useful in the treatment of many infectious diseases such as urinary tract infection, acne and many other skin diseases.
本研究的目的是评价千层属植物部分(叶)油提取物对细菌菌株的抑菌活性,以治疗尿路感染等感染性疾病。总的来说,提取法得到的提取物对不同的被试微生物都有抑菌活性。采用琼脂孔扩散法对7株大肠杆菌、2株肺炎克雷伯菌和1株粪肠球菌等10种病原菌进行抑菌活性评价。本研究结果表明,千层木香精油对细菌的抑菌作用明显,抑菌带明显,但药敏试验效果较差。抗生素作为阳性对照,乙醇作为阴性对照。综上所述,千层属植物提取物中所含的化合物对治疗泌尿道感染、痤疮等多种皮肤病有一定的疗效。
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Journal of Plant Pathology & Microbiology
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