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Improved Production of Recombinant Human Activin A in Escherichia coli 重组人激活素A在大肠杆菌中的改良生产
Q3 Multidisciplinary Pub Date : 2021-08-22 DOI: 10.22059/JSCIENCES.2021.316248.1007609
Z. Hajihassan, Navid Nazari, Fatemeh Armaghan
Activin A as a member of transforming growth factor β (TGF-β) superfamily, plays important roles in the body such as regulation of cell growth and differentiation, wound repairing and modulation of inflammatory responses. More importantly, it can be used as a therapeutic agent; so its recombinant production, especially in the periplasmic space of E. coli as an economical bacterium is of great value. The aim of this study is large- scale production of activin A with a correct structure. For this purpose, three strategies were used. First, an efficient and appropriate signal peptide, modified Iranian Bacillus Licheniformis α-amylase signal peptide, was used to secrete activin A to the periplasmic space of E. coli as a suitable environment for correct protein folding. Second, cytoplasmic chaperones, Dnak, DnaJ, GroEL/ GroES, TF (trigger factor) were expressed simultaneously with activin A. And finally, the optimal agitation rate for improved production of activin A was found in the bioreactor scale. Our results indicated that by the co-expression of TF with activin A and using agitation rate of 1000 rpm maximum expression of activin A in E. coli was obtained. More importantly, based on the CD spectroscopy results and bioassay test the recombinant activin A had appropriate secondary structure and was fully functional.
激活素A作为转化生长因子β (TGF-β)超家族成员,在机体中具有调节细胞生长分化、伤口修复、调节炎症反应等重要作用。更重要的是,它可以作为一种治疗剂;因此,它的重组生产,特别是在大肠杆菌的质周空间作为一种经济细菌具有重要的价值。本研究的目的是大规模生产具有正确结构的激活素A。为此,我们使用了三种策略。首先,利用一种高效、合适的信号肽——改良伊朗地衣芽孢杆菌α-淀粉酶信号肽,将激活素A分泌到大肠杆菌的质周空间,为正确折叠蛋白质提供合适的环境。其次,细胞质伴侣、Dnak、DnaJ、GroEL/ GroES、TF(触发因子)与激活素A同时表达。最后,在生物反应器规模上找到了提高激活素A产量的最佳搅拌速率。结果表明,在搅拌速率为1000 rpm时,TF与激活素A共表达,激活素A在大肠杆菌中的表达量最大。更重要的是,通过CD光谱和生物测定测试,重组激活素A具有合适的二级结构,功能齐全。
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引用次数: 0
Religious Restrictions for Sports: الضوابط الشرعية للألعاب الرياضية 对运动的合法管制
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.l250321
Ibrahim Jalil Khalil Ahmad Ibrahim Jalil Khalil Ahmad
This thesis takes into consideration sports and specifies their religious restrictions. It also aims to provide people interested in sport with these religious restrictions which are illustrated through the descriptive and inductive approach. The thesis has arrived at the conclusion that sports are religiously permitted if they have met the religious conditions. In case a condition is missing, the permission turns into hatred or prohibition. The thesis also recommends that Olympic committees, sport federations and sport centers should have a verified book that is referred to in order to know the religious rules for sports. Also, it is recommended that sportsmen and coaches should attend an annual religious seminar to discuss sport and related issues.
本文以体育运动为研究对象,明确了体育运动的宗教限制。它还旨在通过描述和归纳的方法向对体育感兴趣的人提供这些宗教限制。本文得出结论:体育运动在宗教上是被允许的,只要符合宗教条件。如果缺少一个条件,允许就会变成仇恨或禁止。本文还建议奥委会、体育联合会和体育中心应该有一本经过验证的书籍,以便了解体育的宗教规则。此外,建议运动员和教练应参加一年一度的宗教研讨会,讨论体育和相关问题。
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引用次数: 0
Issues of discrepancy between the original and ostensible from the book of inheritance by collection and study: مسائل تعارض الأصل والظاهر في كتاب الفرائض: جمعًا ودراسة
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.y270121
Alaa Ahmad Altayyar Alaa Ahmad Altayyar
  The research aims to collect issues that contradict the origin and the apparent mentioned in the chapter on the obligations, and study them in a comparative jurisprudential study that shows the position of the original, the apparent position and the face of the contradiction, and then weighting one of them with the evidence, and the method used in the study is the inductive and deductive approach. The most important findings of the study is that: There may be a conflict between origins and ostensible such as evidence and the weights in which the conflict occurs, according to the human view, not according to the fact of the matter, because religion does not conflict, if we find that there is a conflict between us, we collect the original and pure and face the conflict even We remove what appeared to us from conflict, it is required to implement the issue three conditions: the union of origin and ostensible in her , and the realization of the conflict between the origin and the apparent, and the difference in the time of the occurrence of the origin and the apparent, and I can not say that the issues i collected are all issues that conflict the origin and the ostensible in these sections, but they are what I have reached by reading, research.
本研究旨在收集义务一章中提到的与起源和表见相矛盾的问题,用比较法学的方法研究它们,表明起源的立场、表见的立场和矛盾的面对,然后用证据对其中一个进行加权,研究的方法是归纳和演绎的方法。研究最重要的发现是:可能会有冲突的起源和证据和权重等表面上的冲突发生时,根据人类的观点,而不是根据事实,因为宗教信仰不冲突,如果我们发现我们之间有冲突,我们收集原始和纯和面对冲突甚至我们消除了冲突,应实现问题三个条件:起源和表面的结合,以及起源和表面之间冲突的实现,以及起源和表面发生时间的差异,我不能说我收集的问题都是这些章节中起源和表面冲突的问题,但它们是我通过阅读,研究得出的结论。
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引用次数: 0
A New Lifetime Model, Stochastic Orders and Kidney Infection Regression model 一种新的生命周期模型、随机顺序与肾脏感染回归模型
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.22059/JSCIENCES.2021.321231.1007639
Araf Khanjari Idenak, M. Zadkarami, S. M. R. Alavi
We introduce a method to generate a new class of lifetime models based on the bounded distributions such that the defined models are exclusively a special case of the new class. A new subfamily, Generalized Alpha Power (GAP) is discussed and some stochastic orders in this subfamily are investigated to identify the proposed method effect. The performance of the maximum likelihood estimators based on the simulation is studied and in the end, the importance and flexibility of the new family for the models are illustrated by a real data set. Our results indicate that using the proposed method substantially improves the fitness of any G-family model and can be extended to any real data set. Finally, the GAPTW regression model is applied to the kidney infection data.
我们提出了一种基于有界分布生成新一类生命周期模型的方法,使得所定义的模型完全是新一类生命周期模型的特例。讨论了一个新的子族——广义阿尔法幂(GAP),并对该子族中的一些随机阶进行了研究,以验证该方法的效果。研究了基于仿真的极大似然估计器的性能,最后通过一个实际数据集说明了新类模型的重要性和灵活性。我们的结果表明,使用该方法大大提高了任何g族模型的适应度,并且可以扩展到任何实际数据集。最后,将GAPTW回归模型应用于肾脏感染数据。
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引用次数: 0
A pause with the Verses of Bahrain in The Holy Quran: وقفة مع آيات البحرين في القرآن الكريم 暂停与巴林在神圣的可兰经的诗句:وقفةمعآياتالبحرينفيالقرآنالكريم
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.a110121
Awad Mohammed Ahmed Kambal Awad Mohammed Ahmed Kambal
The essence of this topic is the conversation about (The Verses of Bahrain) mentioned in the Book of God Almighty, as these verses are mentioned five times in the Qur’an. The honorable one, and that is why this research was the subject of five investigations. The researcher spoke in those investigations about the verses in which the word (Bahrain) came in Deuteronomy, The researcher dealt with the many benefits and blessings of these seas, as their fresh and salty waters have many benefits, and countless and countless goodies, and there is the great divine wisdom in the isthmus and the interdicted stone, which was mentioned in those verses, and the differences of scholars in the past and present in that barrier, and how modern science has interpreted that. In the course of the research, the researcher mentioned the living creatures that live inside these seas, as well as extracting pearls and coral from the depths of these vast oceans, and seeing the large ships that pierce the water in an enormous way back and forth, all with the power of God Almighty. As for the method and nature of the study, it is represented in the inductive analytical method, by collecting the verses in question, explaining and interpreting them according to the pleasure of the Almighty God from ancient and modern sources, and this method is what researchers follow in such research. The most important results of this research can be summarized as follows: The seas have a great variety of importance and that is why they took a good space from the Holy Book. And that the Signs of the Sea include a scientific miracle that modern science recently discovered. That the Prophet, may God’s prayers and peace be upon him, did not explain such verses, because the time has not yet come for them. That such verses clearly indicate the greatness and enormous power of God.
这个话题的本质是关于全能的真主的书中提到的(巴林经文)的对话,因为这些经文在古兰经中被提到了五次。这就是为什么这项研究是五次调查的主题。研究人员在调查中谈到了《申命记》中出现“巴林”这个词的经文,研究人员谈到了这些海洋的许多好处和祝福,因为它们的淡水和咸水有很多好处,还有无数的好处,在这些经文中提到的地峡和被封锁的石头中有伟大的神圣智慧,以及过去和现在的学者在这个障碍上的差异。以及现代科学是如何解释的。在研究过程中,研究员提到了生活在这些海洋中的生物,以及从这些浩瀚的海洋深处提取珍珠和珊瑚,以及看到巨大的船只在水中来回穿梭,这一切都是在全能的上帝的力量下进行的。在研究的方法和性质上,表现为归纳分析法,即从古今文献中收集所研究的经文,按照全能神的旨意进行解释和阐释,这是研究者在研究中所遵循的方法。这项研究的最重要的结果可以概括如下:海洋具有各种各样的重要性,这就是为什么它们从《圣经》中占据了很好的空间。海洋的迹象包括现代科学最近发现的一个科学奇迹。先知,愿真主的祈祷和和平在他身上,没有解释这些经文,因为时间还没有到来。这些经文清楚地表明上帝的伟大和巨大的力量。
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引用次数: 0
Raising children in the Holy Quran: تربية الأولاد في القرآن الكريم
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.l171220
Layla Mohamed Alaqel Layla Mohamed Alaqel
The aim of the research is to explain the method of the Qur'an in raising children, by addressing the texts referring to the verses of raising children in the Holy Quran, citing some hadiths and the positions of prophets and righteous people in raising their children and the ideological, moral and emotional aspects and the rights of children over their parents in education. This research has followed the descriptive approach, and the results of the research were: God sent the prophets to guide people, and among the ways of guiding good education of children, if the Muslim child grew up healthy and correct, it was called to the goodness of the Muslim community as a word, and Islam has taken more care of the education of children than before of nations, and for the sake of raising children goodly, Islam has guaranteed children many Islamic rights, which exceed what the children's rights organizations have adopted together, and I have recommended the importance of instilling faith in the generation. Young, as well as the interest in the educators themselves, as a large number of them do not know the Islamic curriculum in raising children, and also the work of weekly seminars extending a period of time especially for mothers, to introduce them to the importance of education and its correct methods.
本研究的目的是通过引用《古兰经》中关于养育孩子的经文,引用一些圣训和先知和正义人士在养育孩子方面的立场,以及孩子在思想、道德和情感方面的教育和孩子对父母的权利,来解释《古兰经》养育孩子的方法。本研究采用描述性研究方法,研究结果如下:真主派遣先知来引导人们,在引导儿童良好教育的方法中,如果穆斯林儿童健康正确地成长,就被称为穆斯林社区的善良,伊斯兰教比以前的国家更关心儿童的教育,为了培养儿童,伊斯兰教保障了儿童许多伊斯兰权利,这超过了儿童权利组织所采取的总和。我还建议向这一代人灌输信仰的重要性。年轻的,以及对教育者本身的兴趣,因为他们中的很多人不知道伊斯兰教在养育孩子方面的课程,而且每周的研讨会也延长了一段时间,特别是为母亲们,向他们介绍教育的重要性及其正确的方法。
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引用次数: 0
Pluralism and diversity from the Islamic perspective Pluralism and diversity from the Islamic perspective: التعدد والتنوع من المنظور الإسلامي 伊斯兰教视角下的多元主义和多样性:التعدد والتنوع من المنظور الإسلامي
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.r220221
Taghread Yousf Keadan Taghread Yousf Keadan
The aim of this research is to clarify the phenomenon of pluralism and diversity in its various aspects, with a focus on Islam’s position on pluralism and diversity in its various types and divisions. The research also sought to address religious and political pluralism, cultural pluralism, economic pluralism and Islam’s position and outlook on it. The study is based on the descriptive and analytical approach that sheds light on pluralism and diversity from an Islamic perspective, and through the descriptive and analytical approach, the study will provide a description and analysis of the plurality and diversity in thought and culture from the application of Islamic thought. The research reached the following results: - Religious pluralism, or Islam’s view of pluralism in general, is the ideal solution to the problem of religious conflict in the world and to the peaceful coexistence of different religions. Diversity in Islamic society is the best evidence of Islam's tolerance and its recognition that difference is a universal nature. Islam adopts political pluralism on condition that it be disciplined within the framework of commitment to the supremacy of Sharia and not deviating from its established principles. - Islamic economics is based on matters that other systems lack. It combines private and public ownership at the same time, considering that both of them are assets and each of them has its objectives and sources, provided that they are legitimate. Economic pluralism expresses the existence of more than one economic system in a single country, and Islamic law is concerned with the economic aspect.
本研究的目的是澄清多元化和多样性现象的各个方面,重点关注伊斯兰教在其各种类型和分歧中对多元化和多样性的立场。这项研究还设法讨论宗教和政治多元主义、文化多元主义、经济多元主义以及伊斯兰教对此的立场和看法。本研究以描述和分析的方法为基础,从伊斯兰教的角度阐述多元主义和多样性,并通过描述和分析的方法,从伊斯兰思想的应用出发,对思想和文化的多元性和多样性进行描述和分析。研究得出以下结论:-宗教多元主义,或伊斯兰教的多元主义观,是解决世界宗教冲突问题和不同宗教和平共处的理想办法。伊斯兰社会的多样性是伊斯兰教宽容和承认差异是普遍性质的最好证据。伊斯兰采取政治多元化的条件是,它必须在承诺伊斯兰教法至上的框架内受到纪律约束,并且不偏离其既定原则。——伊斯兰经济学的基础是其他体系所缺乏的东西。它同时把私有制和公有制结合起来,考虑到两者都是资产,只要它们是合法的,每一种资产都有其目的和来源。经济多元化表示在一个国家中存在不止一种经济制度,伊斯兰法关注的是经济方面。
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引用次数: 0
The purposes of Islamic sharia politics: مقاصد السياسة الشرعية 伊斯兰教法的目的政治:مقاصدالسياسةالشرعية
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.r050421
Ayman Mohammed Haroush Ayman Mohammed Haroush
In this research a study of the goals of Islamic politics. He talked about the concept of the objectives and their types, and concept of legal politics, then he separated the objectives sought by the street from the jurisprudence of Sharia policy, the researcher wanted to clarify the objectives of politics and highlight the purpose of its jurisprudence, and to show the beauty and merits of the law in this regard, and correct the course of political action to comply with the legitimate objectives. The research was conducted after extrapolating the texts of Sharia and the words of the jurists on the topic of Sharia policy, the legal policy was generally intended to preserve the worldly interests of the people, such as achieving security, services and civilized development, and eschatological interests, such as preserving religion and spreading moral values and science. The research recommended teaching the purposes of Sharia policy to researchers and those working in politics, and need to activate political jurisprudence in contemporary calamities according to the purposes of political law.
本研究对伊斯兰政治的目标进行了研究。他谈到了目标及其类型的概念,以及法律政治的概念,然后他将街头所寻求的目标与伊斯兰教法政策的法理分开,研究者想要澄清政治的目标并突出其法理的目的,并在这方面展示法律的美和优点,并纠正政治行动的过程以符合合法目标。该研究是在对伊斯兰教法文本和法学家关于伊斯兰教法政策主题的言论进行外推后进行的,法律政策一般旨在维护人民的世俗利益,如实现安全,服务和文明发展,以及末世利益,如保存宗教和传播道德价值观和科学。该研究建议向研究人员和从事政治工作的人教授伊斯兰教法政策的目的,并需要根据政治法的目的在当代灾难中激活政治法学。
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引用次数: 0
Effects of commonality in transaction contracts and their provisions – Comparative Jurisprudence Study –: آثار الشيوع في عقود المعاملات وأحكامها – دراسة فقهية مقارنة –
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.e240121
Shamsul Alam Mohammed Fayaz Al-Ghazi Shamsul Alam Mohammed Fayaz Al-Ghazi
This research deals with the rule of benefiting from the common share and its effects on transaction contracts; this research has been presented in five sections. In the first, I explained the commons and its causes with mentioning their types. In the second, I knew the effects of commonality in companies and demonstrated the dispute of jurists regarding the requirement of communality in the profit of the company. The third came indicating the effects of common property in mortgage, including the doctrine of the jurists in that and their evidence and what was mentioned in the discussions, indicating the most correct saying of the scholars. On the fourth, I explained the provisions of common lease between two partners. The fifth section included the doctrines of jurists in the endowment of one of the partners share of the public domain, explaining the evidence of jurists in that and what was mentioned in the discussions and indicated the most correct in it; then it was concluded with a conclusion that included the most important results and recommendations.
本文研究了普通股收益规则及其对交易契约的影响;本研究分为五个部分。在第一篇文章中,我解释了公地及其成因,并提到了它们的类型。在第二部分,我了解了公共性在公司中的作用,并论证了法学家对公共性在公司利润中的要求的争议。第三种观点指出了共同财产在抵押中的作用,包括法学家的学说以及他们的证据和讨论中提到的内容,表明了学者们最正确的说法。第四,我解释了双方共同租赁的条款。第五部分包括了法学家的学说关于其中一个合伙人的公共领域份额的捐赠,解释了法学家的证据以及讨论中提到的内容并指出了其中最正确的;然后得出结论,其中包括最重要的结果和建议。
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引用次数: 0
loyalty in the Holy Quran: الولاء في القرآن الكريم
Q3 Multidisciplinary Pub Date : 2021-06-30 DOI: 10.26389/ajsrp.c231220
Salama Awad Al-Hosan Salama Awad Al-Hosan
This study deals with the issue of loyalty in the Holy Quran. Because of its great importance in the Muslim faith and the impact it entails, and since this loyalty deals with many types, many people differed in its legitimacy, or expanded without restrictions, this study aimed to clarify the meaning and types of loyalty in the Holy Qur’an, and to know its most important aspects and the controls set by the Holy Quran for each of these types. To achieve these goals, the researcher followed: (the inductive approach) to collect and extrapolate the word loyalty in the Noble Qur’an, the (analytical) method to clarify the meaning of this word through its context in the Qur’an, and the (deductive) approach to derive the connotations of this word through the Qur’an context. And this study reached some results, the most important of which are: that loyalty may be to someone other than God in its worldly sense, and it may only be in its absolute meaning to God Almighty.
本研究涉及神圣古兰经中的忠诚问题。由于忠诚在穆斯林信仰中的重要性及其所带来的影响,而且这种忠诚涉及许多类型,许多人在其合法性上存在差异,或者不受限制地扩展,因此本研究旨在澄清《古兰经》中忠诚的含义和类型,并了解其最重要的方面以及《古兰经》对每种类型的控制。为了实现这些目标,研究者采用了归纳法(归纳法)来收集和推断高贵古兰经中的“忠诚”一词;分析法(归纳法)通过古兰经中的上下文来厘清“忠诚”一词的含义;演绎法(归纳法)通过古兰经的上下文来推导“忠诚”一词的内涵。这项研究得出了一些结论,其中最重要的是:忠诚可能是对世俗意义上的上帝以外的人,也可能是对全能的上帝的绝对意义上的忠诚。
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引用次数: 0
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journal of sciences islamic republic of iran
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