: One of the most popular methods for figuring out a drug’s intestinal permeability is the intestinal perfusion (SPIP) approach. Metoprolol as tartrate and phenol red are frequently utilized as reference compounds in perfusion tests to assess the permeability coefficient of the study’s focus component, Domperidone maleate. Our study’s goal was to create and validate a reversed-phase liquid chromatographic technique for the simultaneous detection of Domperidone maleate, metoprolol, and phenol red for use in intestinal perfusion studies. The analysis was performed on a C18 column 150 × 1.5 mm, 5.0 µm using a mobile phase consisting of methanol: 20 mM potassium dihydrogen phosphate buffer 43:57, v/v ; pH 3.1. The procedure was validated using the FDA’s standards for stability, linearity, precision, and accuracy. All calibration curves were linear r 2 > 0.99. The system suitability parameters of the method are the required limits all analytes. The in situ intestinal permeability experiments can easily be standardized with the help of this newly created and proven approach.
肠灌注法(SPIP)是测定药物肠通透性最常用的方法之一。美托洛尔作为酒石酸盐和酚红经常被用作灌注试验的参比化合物，以评估本研究的重点成分马来酸多潘立酮的渗透系数。本研究的目的是建立并验证一种同时检测马来酸多潘立酮、美托洛尔和酚红的反相液相色谱技术，用于肠道灌注研究。色谱柱为C18，色谱柱为150 × 1.5 mm, 5.0µm，流动相为甲醇:20 mm磷酸二氢钾缓冲液43:57,v/v;pH值3.1。该方法使用FDA的稳定性、线性度、精密度和准确度标准进行验证。所有的校准曲线均为线性r 2 bb0 0.99。该方法的系统适宜性参数是所有分析物的要求限值。在这种新方法的帮助下，原位肠通透性实验可以很容易地标准化。
{"title":"A newly developed and fully validated HPLC method for simultenous determination of in-situ single pass intestinal permeability of domperidone, metoprolol tartarate and phenol red","authors":"Saniye Özcan, E. Çağlar, M. Kaynak, N. Okur","doi":"10.29228/jrp.376","DOIUrl":"https://doi.org/10.29228/jrp.376","url":null,"abstract":": One of the most popular methods for figuring out a drug’s intestinal permeability is the intestinal perfusion (SPIP) approach. Metoprolol as tartrate and phenol red are frequently utilized as reference compounds in perfusion tests to assess the permeability coefficient of the study’s focus component, Domperidone maleate. Our study’s goal was to create and validate a reversed-phase liquid chromatographic technique for the simultaneous detection of Domperidone maleate, metoprolol, and phenol red for use in intestinal perfusion studies. The analysis was performed on a C18 column 150 × 1.5 mm, 5.0 µm using a mobile phase consisting of methanol: 20 mM potassium dihydrogen phosphate buffer 43:57, v/v ; pH 3.1. The procedure was validated using the FDA’s standards for stability, linearity, precision, and accuracy. All calibration curves were linear r 2 > 0.99. The system suitability parameters of the method are the required limits all analytes. The in situ intestinal permeability experiments can easily be standardized with the help of this newly created and proven approach.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE ROLE OF METABOLOMICS IN MEDICINAL PLANT SCIENCE","authors":"E. Nemutlu","doi":"10.29228/jrp.380","DOIUrl":"https://doi.org/10.29228/jrp.380","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rahma NAFI’AH, Y. Sumirtapura, S. T. Darijanto, M. Iwo
{"title":"Diffusion and Lipolysis Test on Forskolin Microemulsion Gel","authors":"Rahma NAFI’AH, Y. Sumirtapura, S. T. Darijanto, M. Iwo","doi":"10.29228/jrp.431","DOIUrl":"https://doi.org/10.29228/jrp.431","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"43 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cansu Goncuoglu, Nesligul Ozdemir, Emre Kara, Aygin Bayraktarekincioglu
{"title":"Definition and prevalence over the years of polypharmacy in Turkey: A systematic review","authors":"Cansu Goncuoglu, Nesligul Ozdemir, Emre Kara, Aygin Bayraktarekincioglu","doi":"10.29228/jrp.434","DOIUrl":"https://doi.org/10.29228/jrp.434","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"11 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Piperine and curcumin can be combined in a mixture as piperine has been known as a bioenhancer. The piperine-curcumin combination was formulated in a solid dispersion-based microparticle containing Piper nigrum and Curcuma longa extracts. The aim of the study was to simultaneously determine piperine and curcumin concentrations in a combined dosage form of solid dispersion-based microparticles using UV-Vis spectrophotometry. A UV-Vis spectrophotometry was combined with a partial least square (PLS) approach with a central composite design (CCD) to develop a calibration series consisting of 36 standard mixtures of piperine and curcumin at concentrations ranging from 0 to 6 g/mL. The model of the calibration series was validated for determination coefficient ( R 2 ), root means square of error prediction/cross-validation (RMSEP/RMSECV) dan predicted residual sum of square (PRESS). Accuracy and precision were determined as per ICH guidelines. The PLS model was successfully validated and applied for resolving overlaid spectra of piperine and curcumin at 206 – 408 nm. Accuracy and precision studies of prepared samples containing a mixture of piperine and curcumin at low, medium, and high concentrations conducted on different days were met with the AOAC International requirements. The limit of detection (LOD) was determined using a pseudo-variates model, and the limit was found to be 0.25 µ g/mL and 0.33 µ g/mL for piperine and curcumin, respectively. The proposed method is suitable for simultaneously determining piperine and curcumin that appeared in a mixture of P. nigrum and C. longa extracts in the solid dispersion-based microparticle samples.
{"title":"Chemometrics-assisted UV-spectroscopy for simultaneous determination of curcumin and piperine in solid dispersion-based microparticles containing Curcuma longa and Piper nigrum extracts","authors":"Dewi Setyaningsih, Y. Hartini, D. Notario","doi":"10.29228/jrp.423","DOIUrl":"https://doi.org/10.29228/jrp.423","url":null,"abstract":": Piperine and curcumin can be combined in a mixture as piperine has been known as a bioenhancer. The piperine-curcumin combination was formulated in a solid dispersion-based microparticle containing Piper nigrum and Curcuma longa extracts. The aim of the study was to simultaneously determine piperine and curcumin concentrations in a combined dosage form of solid dispersion-based microparticles using UV-Vis spectrophotometry. A UV-Vis spectrophotometry was combined with a partial least square (PLS) approach with a central composite design (CCD) to develop a calibration series consisting of 36 standard mixtures of piperine and curcumin at concentrations ranging from 0 to 6 g/mL. The model of the calibration series was validated for determination coefficient ( R 2 ), root means square of error prediction/cross-validation (RMSEP/RMSECV) dan predicted residual sum of square (PRESS). Accuracy and precision were determined as per ICH guidelines. The PLS model was successfully validated and applied for resolving overlaid spectra of piperine and curcumin at 206 – 408 nm. Accuracy and precision studies of prepared samples containing a mixture of piperine and curcumin at low, medium, and high concentrations conducted on different days were met with the AOAC International requirements. The limit of detection (LOD) was determined using a pseudo-variates model, and the limit was found to be 0.25 µ g/mL and 0.33 µ g/mL for piperine and curcumin, respectively. The proposed method is suitable for simultaneously determining piperine and curcumin that appeared in a mixture of P. nigrum and C. longa extracts in the solid dispersion-based microparticle samples.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adeltrudis Adelsa DANIMAYOSTU, E. Lukitaningsih, R. Martien, Retno Danarti
: High Performance Liquid Chromatography (HPLC) was used to determine vitamin D3 content from the topical dosage form. Self-nanoemulsifying drug delivery systems (SNEDDS) based hydrogel was aimed to optimize the delivery of vitamin D3 through the skin due to its high lipophilicity. Quantifying vitamin D3 was performed using the internal standard, vitamin D2, on LiChrocart C18 125-4 reverse phase column. Several characteristics of validation were referred including specificity, linearity, range, accuracy, precision, limits of detection, and quantification. The mobile phase was optimized as a mixture of acetonitrile-methanol-water for injection 94:3:3 v/v, and the flow rate was optimized at 0.8 mL/min at 25°C. Vitamin D3 detection and quantification were observed at a wavelength of 266 nm using an ultra-violet (UV) detector. The analysis method performed no excipient interference, including separating vitamin D3 from its matrix. Extraction of vitamin D3 from its matrix was observed using the dialysis membrane, with agitation (vortex) and no agitation (immersion). The drug content determination was found to be 108.22 % ± 14.77% for the immersion method and 72.04% ± 33.37% for the vortex method. Further, the high variability of the results indicated the dialysis membrane was not a suitable method for determining lipophilic drug content from the colloidal polymer matrix. The other techniques with negligible variation, such as reverse dialysis or subsequent solvent addition, are preferred.
{"title":"Determination of Vitamin D3 Loaded Selfnanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel","authors":"Adeltrudis Adelsa DANIMAYOSTU, E. Lukitaningsih, R. Martien, Retno Danarti","doi":"10.29228/jrp.410","DOIUrl":"https://doi.org/10.29228/jrp.410","url":null,"abstract":": High Performance Liquid Chromatography (HPLC) was used to determine vitamin D3 content from the topical dosage form. Self-nanoemulsifying drug delivery systems (SNEDDS) based hydrogel was aimed to optimize the delivery of vitamin D3 through the skin due to its high lipophilicity. Quantifying vitamin D3 was performed using the internal standard, vitamin D2, on LiChrocart C18 125-4 reverse phase column. Several characteristics of validation were referred including specificity, linearity, range, accuracy, precision, limits of detection, and quantification. The mobile phase was optimized as a mixture of acetonitrile-methanol-water for injection 94:3:3 v/v, and the flow rate was optimized at 0.8 mL/min at 25°C. Vitamin D3 detection and quantification were observed at a wavelength of 266 nm using an ultra-violet (UV) detector. The analysis method performed no excipient interference, including separating vitamin D3 from its matrix. Extraction of vitamin D3 from its matrix was observed using the dialysis membrane, with agitation (vortex) and no agitation (immersion). The drug content determination was found to be 108.22 % ± 14.77% for the immersion method and 72.04% ± 33.37% for the vortex method. Further, the high variability of the results indicated the dialysis membrane was not a suitable method for determining lipophilic drug content from the colloidal polymer matrix. The other techniques with negligible variation, such as reverse dialysis or subsequent solvent addition, are preferred.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yeşim Kaya Yaşar, S. Sevgi, Gülin Renda, İrem Çavuşoğlu Nalbantoğlu, M. Duman, Feride Sena Sezen
: Fraxinus species (Oleaceae) are commonly used in the treatment of several inflammatory conditions with pain, such as rheumatism, arthritis, and gout in folk medicine. We aimed to evaluate this ethnobotanical claim through in vivo experiments. The n-hexane, dichloromethane, ethyl acetate and water subextracts were obtained from the methanolic extract, which was prepared from the bark of Fraxinus angustifolia Vahl. The antinociceptive and anti-inflammatory activities of extracts were evaluated in mice by hot plate and formalin-induced edema assays, respectively. Mice were treated with 5-100 mg/kg methanol crude extract and 100 mg/kg subextracts intraperitoneally. Administration of methanol extract decreased paw thickness in doses of 25 and 100 mg/kg. Water and ethyl acetate subextracts decreased paw thickness while water subextract also decreased paw volume compared with the control. Paw thickness values in the dichloromethane and n-hexane subextract groups and paw volume values in the ethyl acetate, dichloromethane, and n-hexane subextract groups were comparable to those in the control group. The latency values were higher in the groups treated with the dichloromethane and n-hexane subextracts than in the control group while the other subextracts did not change the latency values compared to the control group in the hot plate test. In conclusion, the cortex of Fraxinus angustifolia exhibited a significant in vivo anti-inflammatory and antinociceptive activity.
{"title":"The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice","authors":"Yeşim Kaya Yaşar, S. Sevgi, Gülin Renda, İrem Çavuşoğlu Nalbantoğlu, M. Duman, Feride Sena Sezen","doi":"10.29228/jrp.414","DOIUrl":"https://doi.org/10.29228/jrp.414","url":null,"abstract":": Fraxinus species (Oleaceae) are commonly used in the treatment of several inflammatory conditions with pain, such as rheumatism, arthritis, and gout in folk medicine. We aimed to evaluate this ethnobotanical claim through in vivo experiments. The n-hexane, dichloromethane, ethyl acetate and water subextracts were obtained from the methanolic extract, which was prepared from the bark of Fraxinus angustifolia Vahl. The antinociceptive and anti-inflammatory activities of extracts were evaluated in mice by hot plate and formalin-induced edema assays, respectively. Mice were treated with 5-100 mg/kg methanol crude extract and 100 mg/kg subextracts intraperitoneally. Administration of methanol extract decreased paw thickness in doses of 25 and 100 mg/kg. Water and ethyl acetate subextracts decreased paw thickness while water subextract also decreased paw volume compared with the control. Paw thickness values in the dichloromethane and n-hexane subextract groups and paw volume values in the ethyl acetate, dichloromethane, and n-hexane subextract groups were comparable to those in the control group. The latency values were higher in the groups treated with the dichloromethane and n-hexane subextracts than in the control group while the other subextracts did not change the latency values compared to the control group in the hot plate test. In conclusion, the cortex of Fraxinus angustifolia exhibited a significant in vivo anti-inflammatory and antinociceptive activity.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DISCOVERY OF NOVEL HCV NS5B POLYMERASE INHIBITORS BY IN SILICO APPROACHES","authors":"Berin Karaman Mayack","doi":"10.29228/jrp.456","DOIUrl":"https://doi.org/10.29228/jrp.456","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Ischemia/reperfusion (IR) encompasses the processes of organ function damage and oxidative injury depending on the successive blood flow obstruction and removal of the obstruction. Known as a vitamin, riboflavin (Rb) is known to be protective against tissue damage with its antioxidant and anti-inflammatory properties. The aim of this study is to investigate the effect of Rb treatment on bladder contraction dysfunction and tissue damage due to IR. The study was conducted with forty Sprague-Dawley rats. The abdominal aorta of anaesthetized rats was occluded to induce ischemia (60 min) and then allowed reperfusion (60 min). Rb (25 mg/kg), n-acetylcysteine NAC (100 mg/kg) or saline was administered orally 15 min before the IR model immediately. The bladder was assessed by biochemical and histological analysis, and the contractility of the I/R-related bladder was detected by organ bath. Compared to the control group, MDA, MPO and caspase-3 activities increased in the IR group, while GSH levels decreased. MDA, GSH and caspase-3 activities were reversed with Rb treatment, but there was no change in MPO level. Healing of IR-induced edema and oxidant damage with Rb and NAC treatment resulted in improvement of the thinning of the bladder wall. According to these results, it can be said that Rb therapy can regulate IR-induced bladder dysfunction by improving antioxidant properties and tissue damage.
{"title":"Effect of riboflavin on rat bladder contractility and oxidant damage following ischemia/reperfusion","authors":"Büşra Ertaş","doi":"10.29228/jrp.468","DOIUrl":"https://doi.org/10.29228/jrp.468","url":null,"abstract":": Ischemia/reperfusion (IR) encompasses the processes of organ function damage and oxidative injury depending on the successive blood flow obstruction and removal of the obstruction. Known as a vitamin, riboflavin (Rb) is known to be protective against tissue damage with its antioxidant and anti-inflammatory properties. The aim of this study is to investigate the effect of Rb treatment on bladder contraction dysfunction and tissue damage due to IR. The study was conducted with forty Sprague-Dawley rats. The abdominal aorta of anaesthetized rats was occluded to induce ischemia (60 min) and then allowed reperfusion (60 min). Rb (25 mg/kg), n-acetylcysteine NAC (100 mg/kg) or saline was administered orally 15 min before the IR model immediately. The bladder was assessed by biochemical and histological analysis, and the contractility of the I/R-related bladder was detected by organ bath. Compared to the control group, MDA, MPO and caspase-3 activities increased in the IR group, while GSH levels decreased. MDA, GSH and caspase-3 activities were reversed with Rb treatment, but there was no change in MPO level. Healing of IR-induced edema and oxidant damage with Rb and NAC treatment resulted in improvement of the thinning of the bladder wall. According to these results, it can be said that Rb therapy can regulate IR-induced bladder dysfunction by improving antioxidant properties and tissue damage.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"28 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A NEW APPROACH IN DRUG DISCOVERY: NETWORK PHARMACOLOGY","authors":"Neziha Yagmur DİKER, Vahap Murat Kutluay","doi":"10.29228/jrp.454","DOIUrl":"https://doi.org/10.29228/jrp.454","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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