Ayse Esra KARADAG, M. E. Okur, Y. Özhan, Hande Si̇pahi̇, Fatih Demirci
: Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mg/mL each standardized extract was applied to oleic+palmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2% level, followed by 36.4% for M. sylvestris , and S. fruticosa extracts with 27.3% reduction, respectively. Glutathione levels increased to 59.1% when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7%; S. fruticosa extract decreased ALT levels by 53.5% and M. sylvestris extract by 38.5%, whereas the standard resveratrol reduced ALT level by 30.9%, respectively. The AST levels for M. sylvestris extract was 46.5%, compared to resveratrol by 93%. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.
:鼠尾草叶、锦葵花、蒲公英空中部分、车前草种子、天竺葵空中部分和葱球茎被记录为治疗肝脏疾病和不同肝脏疾病的传统用途。以民族植物学文献资料为依据，在分子水平上评估草药治疗肝脏疾病的潜在用途。根据欧洲药典，采用高效液相色谱法制备不同的草药提取物并进行标准化。采用HepG2细胞培养模型，将每种标准提取物0.25 mg/mL分别应用于油酸+棕榈酸诱导的脂肪肝。对比分析ALT、AST、GSH、MDA水平，并对细胞进行Nil Red染色。对MDA的还原活性最高的是芥花提取物，其还原活性为48.2%，其次是牛蒡提取物，其还原活性为36.4%，而牛蒡提取物的还原活性为27.3%。应用苜蓿提取物时，谷胱甘肽含量增加到59.1%。黄芪提取物使培养基中的谷胱甘肽水平提高了49.7%;枸杞提取物降低ALT水平53.5%，山羊草提取物降低ALT水平38.5%，而标准白藜芦醇分别降低ALT水平30.9%。与白藜芦醇相比，西林草提取物的AST含量为46.5%，高出93%。sativum, M. sylvestris和S. fruticosa标准提取物显示出相对较好的相关性和活性，需要进一步进行体内研究。
{"title":"Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis","authors":"Ayse Esra KARADAG, M. E. Okur, Y. Özhan, Hande Si̇pahi̇, Fatih Demirci","doi":"10.29228/jrp.467","DOIUrl":"https://doi.org/10.29228/jrp.467","url":null,"abstract":": Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mg/mL each standardized extract was applied to oleic+palmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2% level, followed by 36.4% for M. sylvestris , and S. fruticosa extracts with 27.3% reduction, respectively. Glutathione levels increased to 59.1% when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7%; S. fruticosa extract decreased ALT levels by 53.5% and M. sylvestris extract by 38.5%, whereas the standard resveratrol reduced ALT level by 30.9%, respectively. The AST levels for M. sylvestris extract was 46.5%, compared to resveratrol by 93%. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69839992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NOSE-TO-BRAIN DRUG DELIVERY OF NANOFORMULATIONS: PREPARATION AND IN VITRO EVALUATION","authors":"Işık ÇELIKKOL, Özge ESIM, Canan HASCICEK","doi":"10.29228/jrp.504","DOIUrl":"https://doi.org/10.29228/jrp.504","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136209753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A comprehensive database analysis of post-translational modifications with chemical and functional categories. A throughout PTM study of 2022 databases","authors":"Nadir Gül, Ahmet Yildiz","doi":"10.29228/jrp.286","DOIUrl":"https://doi.org/10.29228/jrp.286","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"9 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69836288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Moghimipour, Arash Tajmal, Hossein Heidari Kaydan, A. Salimi
{"title":"Analysing the Effect of Permeation Enhancers on the In Vitro Skin Permeability of Propranolol: A Mechanistic Study","authors":"E. Moghimipour, Arash Tajmal, Hossein Heidari Kaydan, A. Salimi","doi":"10.29228/jrp.294","DOIUrl":"https://doi.org/10.29228/jrp.294","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69836499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kartini, Aminatush Sholichah, Kurnia Wahyu Islamiyah, Endang Wahyu Fitriani
{"title":"Formulation and validation of analytical methods for ursolic acid in Plantago major gel preparations","authors":"K. Kartini, Aminatush Sholichah, Kurnia Wahyu Islamiyah, Endang Wahyu Fitriani","doi":"10.29228/jrp.311","DOIUrl":"https://doi.org/10.29228/jrp.311","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69836662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High-fat diet induced cardiac hemodynamic changes: effects of exercise and obestatin","authors":"G. Memi, L. Öztürk, O. Palabıyık","doi":"10.29228/jrp.322","DOIUrl":"https://doi.org/10.29228/jrp.322","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69836996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Burcu ÜNLÜ ENDİRLİK, A. Eken, F. Öztürk, A. Gürbay
{"title":"Assessing perfluorooctanoic acid toxicity in lung, heart, and testis tissues of mice: Evaluation of protective effects of taurine and coenzyme Q10","authors":"Burcu ÜNLÜ ENDİRLİK, A. Eken, F. Öztürk, A. Gürbay","doi":"10.29228/jrp.326","DOIUrl":"https://doi.org/10.29228/jrp.326","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring medicines use patterns and practices among the public in the Gaza strip, Palestine: A qualitative study","authors":"Ashraf ABU MHADI, S. C. Ong, Y. Abed","doi":"10.29228/jrp.329","DOIUrl":"https://doi.org/10.29228/jrp.329","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"20 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69837386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium","authors":"D. Kavitha, R. Padmini, T. Deepan, M. Dhanaraju","doi":"10.29228/jrp.360","DOIUrl":"https://doi.org/10.29228/jrp.360","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Employment of liquisolid approach in the development optimization and evaluation of an oral disintegrating tablet of aripiprazole","authors":"Vishranth N, P. Sudheer, D. R, V. S, I. Pasha","doi":"10.29228/jrp.362","DOIUrl":"https://doi.org/10.29228/jrp.362","url":null,"abstract":"","PeriodicalId":17096,"journal":{"name":"Journal of Research in Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69838550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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