Pub Date : 2000-01-01DOI: 10.3358/SHOKUEISHI.41.137
T. Matsuoka, Y. Kawashima, H. Akiyama, H. Miura, Y. Goda, Y. Kusakabe, K. Isshiki, M. Toyoda, A. Hino
A method using polymerase chain reaction (PCR) was designed for the detection of genetically modified maize (GM-maize). There are four lines of GM-maize imported from the United States, and the presence of recombinant deoxyribonucleic acid (DNA) in the maize could be detected with four pairs of specific oligonucleotide primers designed from the sequences of the newly introduced genes. The maize zein gene was also detected as an internal control. This method allows specific detection of each of Bt11, Event176, MON810 and LIBERTY by using pairs of specific primers designed to amplify a segment including part of the exogenously introduced sequence and part of the intrinsic maize sequence. The detection sensitivity was about 0.05% for Event176, MON810 and LIBERTY, and about 0.01% for Bt11. To distinguish among three insect-resistant GM-maize lines, we designed a multiplex PCR method. These three GM-maize lines were distinguishable on the basis of the expected lengths of their amplicons.
{"title":"A method of detecting recombinant DNAs from four lines of genetically modified maize.","authors":"T. Matsuoka, Y. Kawashima, H. Akiyama, H. Miura, Y. Goda, Y. Kusakabe, K. Isshiki, M. Toyoda, A. Hino","doi":"10.3358/SHOKUEISHI.41.137","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.137","url":null,"abstract":"A method using polymerase chain reaction (PCR) was designed for the detection of genetically modified maize (GM-maize). There are four lines of GM-maize imported from the United States, and the presence of recombinant deoxyribonucleic acid (DNA) in the maize could be detected with four pairs of specific oligonucleotide primers designed from the sequences of the newly introduced genes. The maize zein gene was also detected as an internal control. This method allows specific detection of each of Bt11, Event176, MON810 and LIBERTY by using pairs of specific primers designed to amplify a segment including part of the exogenously introduced sequence and part of the intrinsic maize sequence. The detection sensitivity was about 0.05% for Event176, MON810 and LIBERTY, and about 0.01% for Bt11. To distinguish among three insect-resistant GM-maize lines, we designed a multiplex PCR method. These three GM-maize lines were distinguishable on the basis of the expected lengths of their amplicons.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"25 1","pages":"137-143"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72683074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biological method for determination of residual benzylpenicillin in livestock products.","authors":"K. Fujita, Kanako Yamaguchi, S. Matsuoka, M. Takayama, M. Watai, K. Tanno, N. Maruyama, M. Murayama, M. Toyoda","doi":"10.3358/SHOKUEISHI.41.149","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.149","url":null,"abstract":"畜産食品中のベンジルペニシリン (PCG) の微生物学的試験法について検討した. 水で抽出し, タングステン酸により除タンパクした後, Bond Elut C18で精製を行った. 定量は円筒平板法を用い, 同定はマイクロバイオオートグラフィーにより実施した. 本法によるCodex規格の残留基準値レベルでの平均回収率は, 80.7%であり, 定量下限は肉, 肝臓, 腎臓で5ppb, 牛乳で1ppbであった.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"24 26 1","pages":"149-153"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88709783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Method-performance studies of notified analytical method for esprocarb and other 4 pesticides.","authors":"K. Sasaki, T. Tatsuno, Munetomo Nakamura, T. Imazawa, T. Ohshima, Y. Kondo, H. Tagata, M. Chiba, R. Matsuda, M. Toyoda","doi":"10.3358/SHOKUEISHI.41.219","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.219","url":null,"abstract":"エスプロカルブ等5農薬の告示試験法評価のために共同実験を行った. 6分析機関で5農薬を添加した玄米等8作物を分析したときの各農薬回収率の平均値は87.2～102.2%, 併行再現性及び室間再現性の相対標準偏差はそれぞれ1.1～9.6%, 7.9～17.3%と良好な結果が得られた. 検出限界はピリブチカルブでは0.0001～0.01μg/g, 他の4農薬では0.00005～0.005μg/gの範囲であった.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"49 48 1","pages":"219-223"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80301473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of nonylphenol in fish on the market.","authors":"S. Nemoto, S. Takatsuki, K. Sasaki, M. Toyoda","doi":"10.3358/SHOKUEISHI.41.377","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.377","url":null,"abstract":"6店舗で購入した市販魚のノニルフェノール (NP) 汚染を調査した結果, 35検体中24検体から9～800ng/gのNPが検出された (検出限界8ng/g). 購入した店舗間で魚のNP汚染に差が認められたことから, 包装材のn-ヘプタンによる溶出試験を行った. その結果, NPが検出された魚の包装に使用されていたラップフィルムから70～931ng/cm2のNPが溶出したが, NP不検出の魚の包装ラップフィルムからはほとんど溶出しなかった. 耐衝撃性ポリスチレン製トレイからもNPが溶出したが, トレイと魚汚染レベルとの関連性は低かった. これらの結果から魚のNP汚染には包装材, 特にラップフィルムからの移行の関与が強く示唆された.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"57 1","pages":"377-380"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79400127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-01-01DOI: 10.3358/SHOKUEISHI.41.321
Yukio Saito, A. Yamamoto, S. Kodama, M. Ohto, T. Ohura, A. Matsunaga
{"title":"Rapid determination of pesticide residues in wines by GC-ECD with large-volume injection system.","authors":"Yukio Saito, A. Yamamoto, S. Kodama, M. Ohto, T. Ohura, A. Matsunaga","doi":"10.3358/SHOKUEISHI.41.321","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.321","url":null,"abstract":"","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"128 1","pages":"321-325"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86187752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-01-01DOI: 10.3358/SHOKUEISHI.41.397
T. Hagiwara, T. Yasuno, Kazuo Saito
{"title":"Determination of total bromine in natural food additives.","authors":"T. Hagiwara, T. Yasuno, Kazuo Saito","doi":"10.3358/SHOKUEISHI.41.397","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.397","url":null,"abstract":"","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"EM-22 1","pages":"397-400"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84526703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of cyclosulfamuron in agricultural products by HPLC.","authors":"Masako Ito, T. Nagayama, I. Takano, Maki Kobayashi, Yasuhiro Tamura, Y. Tateishi, N. Kimura, K. Kitayama, K. Yasuda","doi":"10.3358/SHOKUEISHI.41.371","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.371","url":null,"abstract":"紫外吸収検出器付きHPLCを用いたシクロスルファムロン分析法について検討した. 農産物中のシクロスルファムロンはアセトンで抽出し, 酢酸エチル-n-ヘキサン等量混液に転溶した. 酢酸エチル-n-ヘキサン層を減圧留去後, 残留物をアセトン-n-ヘキサン (1 : 19) 混液に溶解し, ACCUCATカートリッジカラムで精製した. シクロスルファムロンは, UV検出器 (波長260nm) を装着したHPLCにより精度良く測定できた. 0.10μg/g添加時における回収率はほぼ80%以上, 標準偏差はおおむね10未満であり, 検出限界は0.01μg/gであった.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"172 1","pages":"371-376"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76673445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production of toxic metabolites by Penicillium strains isolated from mold-ripened cheese.","authors":"T. Shimada, M. Ichinoe","doi":"10.3358/SHOKUEISHI.41.126","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.126","url":null,"abstract":"輸入及び国産ナチュラルチーズより分離したPenicillium roqueforti は, 2%酵母エキス-50%ショ糖液体培地 (YES) にて供試37菌株中16菌株にマイコフェノール酸の生産性を認めた. 供試37菌株のうち, 14℃培養でロックフォール由来5菌株 (14.7～55.0μg/mL) 及びダナブルー由来2菌株 (5.3, 17.7μg/mL) にマイコフェノール生産能を認め, 培養温度14℃の方が25℃より高生産量であった. 国産青かびチーズ由来菌では10菌株中9菌株に4.1～12.2μg/mLのマイコフェノール酸生産能があった. 一方, P. camemberti はYES培地25℃培養で供試20菌株中すべての菌株に痕跡量～6.09μg/mLのサイクロピアゾン酸の生産能がみられ, 培養温度14℃の方が25℃よりも低生産量であった.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"20 1","pages":"126-132"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80628710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of oxytetracycline in meat and fish using metal chelate resin for clean-up.","authors":"N. Takeda, Hiroki Nishiumi","doi":"10.3358/SHOKUEISHI.41.364","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.364","url":null,"abstract":"畜水産食品中のオキシテトラサイクリン (OTC) をHPLCで分析するために, 0.25%メタリン酸-メタノール-アセトニトリル (6 : 2 : 2) を抽出溶媒とし, 銅イオン負荷金属キレート樹脂を利用した迅速で簡便な前処理法と, 移動相からMg2+を除去してOTCピークの消失を視認できる簡易なオンライン確認法を確立した. 食肉類及び水産食品など9種類の試料を用いた添加回収実験 (0.1及び1μg/g) での平均回収率は80%以上, 定量下限は0.02μg/gであった. 市販畜水産食品40試料を分析し, うなぎ浦焼 (0.04μg/g) とえび (0.39μg/g) からOTCを検出した. 陽性試料は開発したオンライン確認法で確認を行った.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"43 1","pages":"364-367"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81173128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-01-01DOI: 10.3358/SHOKUEISHI.41.242
I. Takano, T. Nagayama, Maki Kobayashi, Masako Ito, Yasuhiro Tamura, Chieko Takada, N. Kimura, K. Kitayama, K. Yasuda
{"title":"A simplified HPLC procedure for the determination of glyphosate in soybeans employing postcolumn fluorescence labeling.","authors":"I. Takano, T. Nagayama, Maki Kobayashi, Masako Ito, Yasuhiro Tamura, Chieko Takada, N. Kimura, K. Kitayama, K. Yasuda","doi":"10.3358/SHOKUEISHI.41.242","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.242","url":null,"abstract":"","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":"41 1","pages":"242-245"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77334455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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