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2010年4月から2014年11月の岡崎市におけるジビエ(イノシシおよびシカ)のE型肝炎ウイルス感染状況調査 2010年4月至2014年11月在冈崎市的野猪和鹿的E型肝炎病毒感染情况调查
Pub Date : 2015-12-25 DOI: 10.3358/SHOKUEISHI.56.252
邦彦 中根, 寛将 伊藤, 健治 磯谷, 裕子 板倉, 慶一 糟谷, 慎一 小林
: Cases of hepatitis E have been caused by infection with hepatitis E virus (HEV) due to consumption of raw or undercooked game meats and liver of wild boars or deer in various countries. We investigated HEV prevalence in wild boar and deer in Okazaki City and its outskirts, Aichi, Japan, using liver samples (439 boar; 185 deer) collected between April 2010 and November 2014. HEV RNA was detected in 49 (11.2%)of 439 and 0 of 185 samples of wild boar and deer, respectively. The positive rate (13.0%, 28/216) of HEV RNA from the wild boar with estimated body weight of less than 40 kg was significantly higher than that (2.7%, 3/111) in animals with estimated body weight of more than 40 kg. The 49 HEV strains were typed as genotype 4 (G4) by phylogenetic analysis. They clustered with Aichi/Shizuoka strains and 48 of the 49 strains subclustered together (Okazaki strains).
在许多国家,戊型肝炎病例是由于食用生的或未煮熟的野味和野猪或鹿的肝脏而感染戊型肝炎病毒(HEV)引起的。我们调查了日本爱知县冈崎市及其郊区野猪和鹿的HEV流行情况,使用肝脏样本(439头野猪;在2010年4月至2014年11月期间收集的185只鹿。在439份野猪和185份鹿样本中分别检测到49份(11.2%)和0份(11.2%)HEV RNA。估计体重小于40 kg的野猪HEV RNA阳性率(13.0%,28/216)显著高于估计体重大于40 kg的野猪(2.7%,3/111)。系统发育分析将49株HEV分型为基因4型(G4)。它们与爱知/静冈菌株聚集在一起,49株中有48株亚聚集在一起(冈崎菌株)。
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引用次数: 4
食中毒原因究明のための遺伝子解析によるキノコ鑑別[食衛誌 53 (5),237~242] 为了查明食物中毒原因,通过基因分析鉴别蘑菇。[食卫志53 (5),237 ~ 242]
Pub Date : 2013-04-25 DOI: 10.3358/SHOKUEISHI.54.172
敦 昌山, 太郎 村上, 大輔 佐久間, 雅美 紀, 哲夫 山野, 充 清水
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引用次数: 0
GMダイズ検査におけるGM quickerの有用性について[食衛誌 53 (1),39~ 44(2012)] 关于GM quicker在GM大豆检测中的有效性[食卫杂志53(1),39至44 (2012)]
Pub Date : 2012-04-25 DOI: 10.3358/SHOKUEISHI.53.128
徳子 佐藤, 義紹 杉浦, 敏嗣 田中
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引用次数: 0
Effect of Nisin (Nisaplin) on the Growth of Listeria monocytogenes in Karashi-mentaiko (Red-pepper Seasoned Cod Roe) [食衛誌.50, 173~177 (2009)] Nisin (Nisaplin)对红椒调味鳕鱼籽中单核增生李斯特菌生长的影响[j]。50, 173~177 (2009)]
Pub Date : 2009-10-25 DOI: 10.3358/SHOKUEISHI.50.278
H. Hara, Y. Ohashi, T. Sakurai, K. Yagi, T. Fujisawa, S. Igimi
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引用次数: 0
共同試験による野菜・果実中残留農薬の多成分一斉分析法の評価 [食衛誌.45, 165~174 (2004)] 通过共同试验的蔬菜·果实中残留农药的多成分一齐分析法的评价[食卫志。45,165 ~ 174 (2004)]
Pub Date : 2004-08-25 DOI: 10.3358/SHOKUEISHI.45.218
芳久 柿本, 義隆 苗床, 弘幸 原, 信 宮武, 新介 佐藤, 久子 龍口, 良一 高畠, 亮介 矢本, 照雄 條
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引用次数: 0
内分泌かく乱物質候補ビスフェノールA,スチレンモノマーによるトリプトファン-ニコチンアミド転換経路のかく乱作用 内分泌紊乱物质候选双酚A,苯乙烯单体的色氨酸-烟酰胺转换途径的紊乱作用
Pub Date : 2004-02-25 DOI: 10.3358/SHOKUEISHI.45.1
努 福渡, 舞 鳥落, 万理 太田, 隆造 佐々木, 克己 柴田
内分泌かく乱物質候補であるビスフェノールAとスチレンモノマーがトリプトファン-ニコチンアミド代謝に及ぼす影響を調べた.1% ビスフェノールA含有食の投与により,トリプトファン-ニコチンアミド転換率は対照群の1/15に低下した.尿中のトリプトファン代謝産物量の分析より,キヌレニンから 3-ヒドロキシキヌレニンへの反応に関与するキヌレニンモノヒドロキシラーゼにビスフェノールAが作用することが推察された.1% スチレンモノマー含有食の投与により,転換率は軽度ではあるが有意に低下した.
研究了内分泌紊乱物质候选双酚A和苯乙烯单体对色氨酸-烟酰胺代谢的影响。使用含有1%双酚A的食物后,色氨酸-烟酰胺转化率降至对照组的1/15。通过对尿中色氨酸代谢产物量的分析,可以推测出双酚A作用于由绢糠氨酸向3-羟基绢糠氨酸反应的绢糠氨酸单羟化酶。使用含有1%苯乙烯单体的食物后,转化率虽有轻度但显著降低。
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引用次数: 5
クルマエビ(Penaeus japonicus)におけるオキシテトラサイクリン及びオキソリン酸の残留性と調理による影響 车虾(Penaeus japonicus)中羟四环素和磷酸盐的残留和烹调的影响
Pub Date : 2002-04-25 DOI: 10.3358/SHOKUEISHI.43.62
Kazuaki Uno
クルマエビにオキシテトラサイクリン(OTC)及びオキソリン酸(OA)を経口投与(50 mg/kg)し,それらの残留性について検討した.更に,加熱調理が魚体内の残留薬物に及ぼす影響を検討した.OTCの消失半減期(T1/2)は血リンパでは45時間,筋肉では47時間であった.一方,OAのT1/2は血リンパで55時間及び筋肉で108時間であった.OTCでは,残留基準値以下になるのに要する時間は血リンパ及び筋肉でそれぞれ投薬後8.1日及び7.4日と算出された.OAでは,検出限界値以下になるのに要する時間は血リンパで投薬後21日及び筋肉で29日と算出された.これらは休薬期間以内であった.しかし,殻における両薬物は休薬期間以上に残留すると考えられた.また,加熱調理により両薬物とも残留濃度は減少したが,完全に消失しなかった.
给车虾口服(50mg /kg)羟四环素(OTC)和磷酸盐(OA),对它们的持久性进行了研究。此外,研究了加热烹调对鱼体内残留药物的影响。OTC的消失半衰期(t1 /2)在血淋巴为45小时,在肌肉为47小时。而OA的t1 /2在血淋巴和肌肉的时间分别为55小时和108小时。OTC计算出,血淋巴和肌肉达到残留标准值以下所需的时间分别为用药后8.1天和7.4天。OA计算出,达到检测极限值以下所需的时间在血淋巴和肌肉中分别为用药后21天和29天。这些都在停药期间内。但是,壳中的两种药物在停药期间会残留。另外,通过加热烹调,两种药物的残留浓度均有所减少,但并未完全消失。
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引用次数: 10
遺伝子組換えジャガイモ (NewLeaf Plus® Potato) からの組換え遺伝子検知法の確立及びスナック菓子からの検知 基于基因重组土豆(NewLeaf Plus®Potato)的重组基因检测方法的建立以及对膨化食品的检测
Pub Date : 2002-02-25 DOI: 10.3358/SHOKUEISHI.43.24
Hiroshi Akiyama, Kazue Sugimoto, Misao Matsumoto, Kazuto Isuzugawa, Masaaki Shibuya, Yukihiro Goda, M. Toyoda
日本で安全性審査未終了の遺伝子組換えジャガイモ(NewLeaf Plus® potato; NL-P)について,ポリメラーゼ連鎖反応(PCR)を用いた検知法を検討した.陽性対照プライマー対は,Potato sucrose synthase遺伝子を認識するものを用いた.ジャガイモ葉巻ウイルスのreplicase (PLRV-rep)遺伝子を認識するプライマー対によりPCRを行った結果,NL-Pに特異的なバンドが検出された.更に擬陽性を避けるために,2生物種由来の連続した遺伝子領域を増幅するプライマー対を設計し,特異的にNL-Pが検知されることが明らかとなった.確立したPCR検知法をジャガイモ加工品25検体に応用したところ,スナック菓子3検体からNL-Pが検出された.
在日本尚未完成安全性审查的转基因土豆(NewLeaf Plus®potato;NL-P)方面,研究了采用聚合酶链式反应(PCR)的检测方法。阳性对照引物对使用了识别Potato sucrose synthase基因的引物。利用识别马铃薯雪茄病毒replicase (PLRV-rep)基因的引体对进行PCR的结果,检测出NL-P特异性带。为了进一步避免拟阳性现象,研究人员设计了对来自2个生物物种的连续基因区域进行扩增的引体对,从而可以特异性地检测NL-P。将确立的PCR检测法应用于25个土豆加工品中,结果在3个零食样品中检测出NL-P。
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引用次数: 27
[Multiresidue analysis of organophosphorus pesticides in vegetables and fruits using dual-column GC-FPD, -NPD]. [双柱GC-FPD, -NPD法分析蔬菜水果中有机磷农药的多重残留]。
Pub Date : 2001-12-25 DOI: 10.3358/SHOKUEISHI.42.385
英二 上野, 晴美 大島, 勲 斎藤, 浩 松本
野菜・果実中の農薬残留モニタリングのための多成分分析法を検討した.極性農薬を含む63種の有機リン系農薬を対象として,アセトニトリル抽出,メスシリンダーを用いたろ過・塩析及び有機層の分取操作に加えて,GPCの農薬流出画分を2分し,それにシリカゲル及びフロリジルミニカラムを組み合わせた精製条件を確立し,多数の試料を連続してGC測定することが可能となった.GC装置には主として定量用にRtx®-OPPesticides (FPD, Pモード)を,確認用にRtx®-200 (NPD)を用いたデュアルカラム-デュアル検出器方式を採用し,1回の注入により対象としたすべての農薬の迅速な分析を可能とした.添加回収率は全農薬で 70% 以上,相対標準偏差は60種の農薬で 20% 未満,検出限界は0.5~2 ppbと良好であった.本法を用いて野菜・果実中の農薬残留モニタリングを行ったところ30検体中20検体から15種類の農薬(平均2.0種類/検体)が検出された.
研究了用于蔬菜、果实中农药残留监测的多成分分析法。以包括极性农药在内的63种有机磷农药为对象,除了进行乙腈提取、使用雌缸的过滤和盐析以及有机层的分离操作之外,还对GPC的农药流出画进行了2份。另外,还确立了有机硅和氟氧化锆的组合提纯条件,可以连续对多个样品进行GC测定。GC装置主要采用定量用Rtx®-OPPesticides (FPD, P模式),确认用Rtx®-200 (NPD)的双列-双检测器方式,通过1次的注入可以迅速分析作为对象的所有农药。所有农药的添加回收率均在70%以上,60种农药的相对标准差小于20%,检出界限为0.5 ~ 2ppb,表现良好。利用本法对蔬菜、水果中的农药残留进行了监测,在30个样品中从20个样品中检测出15种农药(平均2.0种/样品)。
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引用次数: 18
全国の行政検査結果(1998年度)を基にした食品中の防かび剤の濃度実態及び摂取量の推定 以全国行政检查结果(1998年)为基础的食品中防霉剂的浓度实态及摄取量的推测。
Pub Date : 2001-12-25 DOI: 10.3358/SHOKUEISHI.42.404
肇 石綿, 基弘 西島, 喜延 深澤
The mean concentration and daily intake of five preservatives were estimated based on the results of an analysis of 89,927 samples of food obtained in official inspections by Japanese local governments in fiscal year 1998. The mean concentration of benzoic acid was 9.5% of the allowable limit, and those of dehydroacetic acid, p-hydroxybenzoic acid, propionic acid, and sorbic acid were 1.5%, 5.7%, 1.7%, and 23.9%, respectively. Daily intake levels of these preservatives per person estimated from the concentration and daily consumption of foods were 6.23 mg, 0.0303 mg, 1.02 mg, 8.10 mg, and 25.0 mg, respectively, and assuming a body weight of 50 kg, the amounts of benzoic acid, p-hydroxybenzoic acid, and sorbic acid consumed were 2.5%, 0.2%, and 2.0% of their acceptable daily intakes, respectively. These values were similar to those obtained based on the results of the official inspections in fiscal years 1994 and 1996.
这五种防腐剂的平均浓度和每日摄入量是根据1998财政年度日本地方政府在官方检查中获得的89,927份食品样本的分析结果得出的。苯甲酸的平均浓度为允许限量的9.5%,脱氢乙酸、对羟基苯甲酸、丙酸和山梨酸的平均浓度分别为1.5%、5.7%、1.7%和23.9%。这些防腐剂的人均每日摄入量分别为6.23毫克、0.0303毫克、1.02毫克、8.10毫克和25.0毫克,假设体重为50公斤,苯甲酸、对羟基苯甲酸和山梨酸的摄入量分别为其每日可接受摄入量的2.5%、0.2%和2.0%。这些数值与根据1994和1996财政年度官方视察结果所得的数值相似。
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引用次数: 1
期刊
Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)
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