Pub Date : 2001-02-25DOI: 10.3358/SHOKUEISHI.42.33
S. Kawasaki, Bon Kimura, Tateo Fujii
We evaluated the TaqMan PCR Salmonella amplification/detection kit (PE Applied Biosystems) for rapid detection of Salmonella from a variety of meat samples. This system uses the 5' nuclease activity of Taq DNA polymerase, which digests an internal fluorogenic probe to monitor the amplification of the target gene. The detection sensitivity of the kit, using 2 kinds of DNA extraction protocols, was compared with that obtained with 4 protocols of official culture methods. A total of 98 meat samples (16 raw beef, 31 pork and 51 chicken) were tested. The results of the TaqMan PCR method and the combined results of the 4 cultural protocols showed excellent agreement. However, no single culture protocol showed optimal recovery of Salmonella comparable to the PCR method. These results suggest that the TaqMan PCR method is a reliable and rapid method useful for detecting Salmonella in meat products.
{"title":"TaqMan™ Salmonella Amplification/Detectionキットと公定法による食肉中からのサルモネラ検出比較","authors":"S. Kawasaki, Bon Kimura, Tateo Fujii","doi":"10.3358/SHOKUEISHI.42.33","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.42.33","url":null,"abstract":"We evaluated the TaqMan PCR Salmonella amplification/detection kit (PE Applied Biosystems) for rapid detection of Salmonella from a variety of meat samples. This system uses the 5' nuclease activity of Taq DNA polymerase, which digests an internal fluorogenic probe to monitor the amplification of the target gene. The detection sensitivity of the kit, using 2 kinds of DNA extraction protocols, was compared with that obtained with 4 protocols of official culture methods. A total of 98 meat samples (16 raw beef, 31 pork and 51 chicken) were tested. The results of the TaqMan PCR method and the combined results of the 4 cultural protocols showed excellent agreement. However, no single culture protocol showed optimal recovery of Salmonella comparable to the PCR method. These results suggest that the TaqMan PCR method is a reliable and rapid method useful for detecting Salmonella in meat products.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91541706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-12-25DOI: 10.3358/SHOKUEISHI.41.368
和寛 菅本, 望 江藤
Cytotoxicity of 4-hydroxy-2E-hexenal (HHE), a hepatotoxic aldehyde, to Chinese hamster lung fibroblast V79 and Balb/c mouse semi-normal cell line BALB/3T3 clone A31 cells was examined. The 50%-inhibitory concentrations to V79 and A31 were 3 μmol/L and 16 μmol/L, respectively. Judging from the sensitivity, HHE might show cytotoxicity below these concentrations. Changes in HHE and malonaldehyde (MA) contents were also examined in yellowtail meat stored at -20°C. HHE content increased up to 60.82±4.39 μmol/kg meat during 28 weeks of storage. In contrast to HHE, MA content increased only slightly in the meat during 28 weeks of storage. From the viewpoint of food hygiene, it is noteworthy that the HHE content increased even when meat was stored at -20°C.
研究了4-羟基- 2e -己烯醛(HHE)对中国仓鼠肺成纤维细胞V79和Balb/c小鼠半正常细胞系Balb/ 3T3克隆A31细胞的细胞毒性。对V79和A31的50%抑制浓度分别为3 μmol/L和16 μmol/L。从敏感性来看，低于这些浓度的HHE可能表现出细胞毒性。在-20℃条件下，检测了黄尾肉HHE和丙二醛(MA)含量的变化。贮藏28周后，HHE含量达到60.82±4.39 μmol/kg。与HHE相比，贮藏28周期间，肉中MA含量仅略有增加。从食品卫生的角度来看，值得注意的是，即使肉类在-20°C下保存，HHE含量也有所增加。
{"title":"脂質過酸化由来アルデヒド, 4-ヒドロキシヘキセナールの細胞毒性及び凍結貯蔵ブリ筋肉中での変動","authors":"和寛 菅本, 望 江藤","doi":"10.3358/SHOKUEISHI.41.368","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.368","url":null,"abstract":"Cytotoxicity of 4-hydroxy-2E-hexenal (HHE), a hepatotoxic aldehyde, to Chinese hamster lung fibroblast V79 and Balb/c mouse semi-normal cell line BALB/3T3 clone A31 cells was examined. The 50%-inhibitory concentrations to V79 and A31 were 3 μmol/L and 16 μmol/L, respectively. Judging from the sensitivity, HHE might show cytotoxicity below these concentrations. Changes in HHE and malonaldehyde (MA) contents were also examined in yellowtail meat stored at -20°C. HHE content increased up to 60.82±4.39 μmol/kg meat during 28 weeks of storage. In contrast to HHE, MA content increased only slightly in the meat during 28 weeks of storage. From the viewpoint of food hygiene, it is noteworthy that the HHE content increased even when meat was stored at -20°C.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84446563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of acibenzolar-S-methyl and its degradation product in agricultural products by HPLC","authors":"S. Takatsuki, S. Nemoto, T. Tsutsumi, K. Sasaki, M. Toyoda","doi":"10.3358/SHOKUEISHI.41.381","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.381","url":null,"abstract":"HPLCによる農産物中のアシベンゾラル-S-メチル (BTH) 及び分解物のbenzo[1,2,3]thiodiazole-7-carboxylic acid (BTC) の分析法を確立した. 試料からリン酸緩衝液 (pH 8.0) とアセトンで抽出後, BTHは塩基性溶液 (pH7.5～8.5) からエーテル-ヘキサン (1 : 1) で再抽出し, BTCはリン酸酸性溶液 (<pH 3) から同溶媒で再抽出した. BTHは脱脂後, フロリジルで精製した. BTCは, 直接フロリジルで精製した. UV検出器付きHPLC及びLC/MSで測定を行った. 米, 麦及びとうもろこしにBTH又はBTCを0.1μg/g添加したときの回収率は, 平均76.8～86.7%, 標準偏差は0.3～2.7であった. 検出限界はUV検出器付きHPLCで0.003μg/g (BTH) 及び0.008μg/g (BTC) であった.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74847705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-10-25DOI: 10.3358/SHOKUEISHI.41.312
K. Monma, K. Sasaki, F. Ushio, Y. Saitoh, Hisatsugu Ichikawa, T. Matsuoka, M. Nishijima, A. Hino
{"title":"Detection Rate of Glyphosate Tolerance Gene in Foreign and Domestic Soybeans and Tofu Products","authors":"K. Monma, K. Sasaki, F. Ushio, Y. Saitoh, Hisatsugu Ichikawa, T. Matsuoka, M. Nishijima, A. Hino","doi":"10.3358/SHOKUEISHI.41.312","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.312","url":null,"abstract":"","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76735956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-10-25DOI: 10.3358/SHOKUEISHI.41.326
T. Chonan, K. Nishimura, Yuzi Hirama
{"title":"Rapid Determination of Residues of Five Kinds of Veterinary Drugs in Livestock Products by HPLC","authors":"T. Chonan, K. Nishimura, Yuzi Hirama","doi":"10.3358/SHOKUEISHI.41.326","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.326","url":null,"abstract":"","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74842697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GC/MS(SIM)による農作物中110農薬の一斉分析法","authors":"S. Nemoto, K. Sasaki, Syuichi Eto, Isao Saito, H. Sakai, Tetsuo Takahashi, Yasuhide Tonogai, T. Nagayama, S. Hori, Yoshiaki Maekawa, M. Toyoda","doi":"10.3358/SHOKUEISHI.41.233","DOIUrl":"https://doi.org/10.3358/SHOKUEISHI.41.233","url":null,"abstract":"農作物からアセトニトリル抽出後, GPC及びカートリッジカラムによるクリーンアップを行い, GC/MS (SIM) 測定する110農薬の一斉分析法を作成した. 6農作物に4又は5分析機関で添加回収試験を行った結果, 各農薬の回収率データ中70～120%の良好な結果を示したデータが全体の70%以上を占めた農薬は77種であった. 各農薬の検出限界値は0.0004～0.08μg/g以下であった.","PeriodicalId":17269,"journal":{"name":"Journal of The Food Hygienic Society of Japan (shokuhin Eiseigaku Zasshi)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72961113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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