The objectives of this study were to the development and technology transfer for local production of Samae Dam Chicken (Gallus gallus) in Uthai Thani Province, Thailand by studying diversity of phenotypic characteristics in the rearing system. The average age of chickens was eight months, randomly numbered 1,000 in Uthai Thani Province. The results showed that variation of quantitative traits such as body weight, body height, body length, body width, wing length, shank length and toes length, the mean is equal to 3.0±0.25 kg/h, 58.5±0.10 cm, 24.5±0.30 cm, 14.5±0.20 cm 33.0±1.15 cm,10.25±0.5 cm and 5.75±0.45 cm respectively. The relationship between qualitative characteristics found that significant strong positive relationships between beak color and shank color (r=0.70; p<0.01), between necklace color and wings feather color (r=0.60; p<0.05), between necklace color and back- necklace color (r=0.15; p<0.01), between wings feathers color and necklace color (r=0.60; p<0.01), between wings feathers color and back-necklace color (r=0.25; p<0.01), between back-necklace color and necklace color (r=0.20; p<0.01), between back-necklace color and wings feather color (r=0.25; p<0.05) and between shank color and beak color. The relationship between quantitative characteristics all positive relationships among the studied quantitative traits was significant (r=0.30 to 0.55; p<0.01). The relationship between qualitative and quantitative factors in which the relationship between characteristics is significant (p<0.05). The positive correlation of beak color with body height (r=0.14), necklace color with bodyweight (r=0.15), necklace color with body width (r=0.17), wings feather color with body length (r=0.23), wings feather color with body width (r=-0.20), wings feather color with wing length (r=-0.14), tail feathers type with bodyweight (r=-0.25), tail feathers type with body height (r=-0.19), tail feathers type with body length (r=-0.26), tail feathers type with body width (r=-0.12), tail feathers type with wing length (r=-0.14), tail feathers type with shank length (r=-0.22), shank color with bodyweight (r=-0.22), shank color with body height (r=-0.18) and shank color with body length (r=-0.22). Effects of qualitative characteristics on quantitative characteristics of the chicken necklace color, wings feather color, back necklace color tail feathers type, shank color had significant difference (p<0.05).
{"title":"Development and identifying quantitative and quantities traits for breeding of Samae Dam Chicken (Gallus gallus), Thailand: Diversity of phenotypic characteristics","authors":"T. Wiyabot, S. Kiattinarueyut","doi":"10.12681/jhvms.27405","DOIUrl":"https://doi.org/10.12681/jhvms.27405","url":null,"abstract":"The objectives of this study were to the development and technology transfer for local production of Samae Dam Chicken (Gallus gallus) in Uthai Thani Province, Thailand by studying diversity of phenotypic characteristics in the rearing system. The average age of chickens was eight months, randomly numbered 1,000 in Uthai Thani Province. The results showed that variation of quantitative traits such as body weight, body height, body length, body width, wing length, shank length and toes length, the mean is equal to 3.0±0.25 kg/h, 58.5±0.10 cm, 24.5±0.30 cm, 14.5±0.20 cm 33.0±1.15 cm,10.25±0.5 cm and 5.75±0.45 cm respectively. The relationship between qualitative characteristics found that significant strong positive relationships between beak color and shank color (r=0.70; p<0.01), between necklace color and wings feather color (r=0.60; p<0.05), between necklace color and back- necklace color (r=0.15; p<0.01), between wings feathers color and necklace color (r=0.60; p<0.01), between wings feathers color and back-necklace color (r=0.25; p<0.01), between back-necklace color and necklace color (r=0.20; p<0.01), between back-necklace color and wings feather color (r=0.25; p<0.05) and between shank color and beak color. The relationship between quantitative characteristics all positive relationships among the studied quantitative traits was significant (r=0.30 to 0.55; p<0.01). The relationship between qualitative and quantitative factors in which the relationship between characteristics is significant (p<0.05). The positive correlation of beak color with body height (r=0.14), necklace color with bodyweight (r=0.15), necklace color with body width (r=0.17), wings feather color with body length (r=0.23), wings feather color with body width (r=-0.20), wings feather color with wing length (r=-0.14), tail feathers type with bodyweight (r=-0.25), tail feathers type with body height (r=-0.19), tail feathers type with body length (r=-0.26), tail feathers type with body width (r=-0.12), tail feathers type with wing length (r=-0.14), tail feathers type with shank length (r=-0.22), shank color with bodyweight (r=-0.22), shank color with body height (r=-0.18) and shank color with body length (r=-0.22). Effects of qualitative characteristics on quantitative characteristics of the chicken necklace color, wings feather color, back necklace color tail feathers type, shank color had significant difference (p<0.05).","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47157518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryptosporidium is an obligate intracellular parasite that infects a wide variety of hosts. Twenty calves with cryptosporidiosis were divided into two groups. A single dose of 2.5 mg/kg buparvaquone was administered intramuscularly to one group, while 100 mg/kg/day paromomycin was administered orally to the other group. The results obtained from the study in which oocyst shedding and clinical parameters of sucking reflex, mental status, oocyst scattering were observed in both groups offer an effective control option with this study, which provides results that support the application of a single dose of buparvaquone injection against the complex treatment methods applied to calves with cryptosporidiosis in the field. In this study, a significant decrease in oocyst shedding and rapid improvement in clinical findings were observed in calves.
{"title":"Comparison of the effects of buparvaquone and paromomycin on oocyst excretion and clinical parameters in diarrheal calves naturally infected with cryptosporidiosis","authors":"FZ Evci, S. Şentürk","doi":"10.12681/jhvms.27509","DOIUrl":"https://doi.org/10.12681/jhvms.27509","url":null,"abstract":"Cryptosporidium is an obligate intracellular parasite that infects a wide variety of hosts. Twenty calves with cryptosporidiosis were divided into two groups. A single dose of 2.5 mg/kg buparvaquone was administered intramuscularly to one group, while 100 mg/kg/day paromomycin was administered orally to the other group. The results obtained from the study in which oocyst shedding and clinical parameters of sucking reflex, mental status, oocyst scattering were observed in both groups offer an effective control option with this study, which provides results that support the application of a single dose of buparvaquone injection against the complex treatment methods applied to calves with cryptosporidiosis in the field. In this study, a significant decrease in oocyst shedding and rapid improvement in clinical findings were observed in calves.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44089202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Şule Çizmeci, AD Dinç, M. Güler, H. Alkan, MB Kıvrak, A. Takcı, MF Çiftçi, ÖF Yeşilkaya, P. Bulut, S. Bulut, A. Zhunushova
Bu çalışmada adjuvan kullanılarak tek doz Subkutan (SC) FSH uygulamasının ve adjuvan kullanılmadan tek doz epidural FSH uygulamasının kan FSH düzeyleri ve süperovülasyon yanıtı üzerine etkilerinin belirlenmesi amaçlanmıştır. Holştayn inekler (n:18) süperovülasyon prosedürü için üç gruba ayrıldı (n = 6). Gruptaki (İM) hayvanlara 4 gün süreyle 12 saat arayla İntramusculer (IM) azaltıcı FSH (Stimufol, 500 μg domuz FSH ve 100 μg domuz LH, Ulg FMV, Liège, Belçika) dozları verildi. (E) grubundaki hayvanlara epidural aralığa FSH (500 µg) verildi. (SC) grubundaki hayvanlara, 10 mL Montanide ISA 206 adjuvanı içinde FSH eklenerek hazırlanan bir karışım Subkutan (SC) ile uygulandı. Overdeki CL sayısı ultrasonografik yöntemle ölçüldü ve 4 CL varlığına göre süperovülasyon belirlendi. FSH ölçümü için tüm hayvanlardan V. jugularis'ten kan örnekleri alındı. Serum FSH (pFSH) seviyeleri ELISA ile belirlendi. Grup 1'deki tüm hayvanlar, süperovülasyon prosedüründen sonra süperovülasyon tepkisine (4>CL) sahipti. 2. grupta sadece bir hayvan yanıt verdi. Grup 3'te, altı hayvandan dördü yanıt verdi. Kan örneklerinin FSH analizinden sonra, IM ve SC uygulamaları arasındaki fark istatistiksel olarak önemsiz iken, IM ve epidural uygulamalar arasındaki fark istatistiksel olarak anlamlıydı. SC ve epidural uygulamalar arasındaki fark önemsizdi.
{"title":"Effects of FSH Administered in Different ways on Superovulation Response and Blood FSH Levels in Cows","authors":"Şule Çizmeci, AD Dinç, M. Güler, H. Alkan, MB Kıvrak, A. Takcı, MF Çiftçi, ÖF Yeşilkaya, P. Bulut, S. Bulut, A. Zhunushova","doi":"10.12681/jhvms.27334","DOIUrl":"https://doi.org/10.12681/jhvms.27334","url":null,"abstract":"Bu çalışmada adjuvan kullanılarak tek doz Subkutan (SC) FSH uygulamasının ve adjuvan kullanılmadan tek doz epidural FSH uygulamasının kan FSH düzeyleri ve süperovülasyon yanıtı üzerine etkilerinin belirlenmesi amaçlanmıştır. Holştayn inekler (n:18) süperovülasyon prosedürü için üç gruba ayrıldı (n = 6). Gruptaki (İM) hayvanlara 4 gün süreyle 12 saat arayla İntramusculer (IM) azaltıcı FSH (Stimufol, 500 μg domuz FSH ve 100 μg domuz LH, Ulg FMV, Liège, Belçika) dozları verildi. (E) grubundaki hayvanlara epidural aralığa FSH (500 µg) verildi. (SC) grubundaki hayvanlara, 10 mL Montanide ISA 206 adjuvanı içinde FSH eklenerek hazırlanan bir karışım Subkutan (SC) ile uygulandı. Overdeki CL sayısı ultrasonografik yöntemle ölçüldü ve 4 CL varlığına göre süperovülasyon belirlendi. FSH ölçümü için tüm hayvanlardan V. jugularis'ten kan örnekleri alındı. Serum FSH (pFSH) seviyeleri ELISA ile belirlendi. Grup 1'deki tüm hayvanlar, süperovülasyon prosedüründen sonra süperovülasyon tepkisine (4>CL) sahipti. 2. grupta sadece bir hayvan yanıt verdi. Grup 3'te, altı hayvandan dördü yanıt verdi. Kan örneklerinin FSH analizinden sonra, IM ve SC uygulamaları arasındaki fark istatistiksel olarak önemsiz iken, IM ve epidural uygulamalar arasındaki fark istatistiksel olarak anlamlıydı. SC ve epidural uygulamalar arasındaki fark önemsizdi.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42555378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Avdatek, HH Demirel, İ. Küçükkurt, D. Acaröz, S. Ince
This study aimed to investigate the protective effect of boron (B) against fat diet (FD)-induced spermatological, histopathological, biochemical and DNA damage in Wistar Albino Rats. Male rats were divided into five groups; the control group was fed a normal diet, the FD group was fed a normal diet with 40% beef tallow the FD+B5 group was fed a fat diet containing 5 mg/kg/daily boron, the FD+B10 group was fed a fat diet containing 10 mg/kg/daily boron, and the FD+B20 group was fed a fat diet containing 20 mg/kg/daily boron. The spermatozoon motility, membrane integrity and glutathione (GSH) level increased in the rats' testicular tissue in the groups given B. Conversely, the abnormal sperm rate, malondialdehyde (MDA) level and DNA damage were high in the FD group. Histopathologically, it was observed that decreased spermatozoa concentration in tubules seminiferous contortus lumen in FD and FD+B5 groups. FD+B10 administration reversed FD-induced spermatological parameters, MDA level and DNA damage in the rats' testes. These results indicated that boron protects spermatological parameters and repairs DNA damage, reduces FD-induced high MDA levels, enhances the antioxidant defense system and eliminates tissue injury in rats' testes.
{"title":"The effects of boron on oxidative stress, reproductive parameters, and DNA damage in testicular tissue in wistar rats on a fatty diet","authors":"F. Avdatek, HH Demirel, İ. Küçükkurt, D. Acaröz, S. Ince","doi":"10.12681/jhvms.27219","DOIUrl":"https://doi.org/10.12681/jhvms.27219","url":null,"abstract":"This study aimed to investigate the protective effect of boron (B) against fat diet (FD)-induced spermatological, histopathological, biochemical and DNA damage in Wistar Albino Rats. Male rats were divided into five groups; the control group was fed a normal diet, the FD group was fed a normal diet with 40% beef tallow the FD+B5 group was fed a fat diet containing 5 mg/kg/daily boron, the FD+B10 group was fed a fat diet containing 10 mg/kg/daily boron, and the FD+B20 group was fed a fat diet containing 20 mg/kg/daily boron. The spermatozoon motility, membrane integrity and glutathione (GSH) level increased in the rats' testicular tissue in the groups given B. Conversely, the abnormal sperm rate, malondialdehyde (MDA) level and DNA damage were high in the FD group. Histopathologically, it was observed that decreased spermatozoa concentration in tubules seminiferous contortus lumen in FD and FD+B5 groups. FD+B10 administration reversed FD-induced spermatological parameters, MDA level and DNA damage in the rats' testes. These results indicated that boron protects spermatological parameters and repairs DNA damage, reduces FD-induced high MDA levels, enhances the antioxidant defense system and eliminates tissue injury in rats' testes.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46801521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Akyuz, A. Kükürt, M. Makav, E. Gökçe, H. Eroğlu, H. Deveci, G. Gökce, M. Karapehlivan
In this study, it was aimed to evaluate total sialic acid (TSA), paraoxonase activity (PON1), malondialdehyde (MDA) and some serum biochemical parameters in cows with subclinical paratuberculosis. Paratuberculosis (PT) test was performed on 500 cows aged 2-6 years by ELISA method. Twenty-four cows diagnosed with paratuberculosis constituted the PT group and 12 healthy cows constituted the control group. According to the results, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and UREA levels were found to be statistically higher (p <0.05) in the group with paratuberculosis compared to the control group. Iron (Fe) levels were found to be lower than the control group (p> 0.05). Compared to the control group, there was a decrease in PON1 activity and a significant increase in TSA and MDA levels in the PT group (p <0.05). In conclusion, in subclinical paratuberculosis infection, the long incubation period and the absence of clinical findings until advanced stages constitute a handicap. However, our findings show that the determination of lipid peroxidation (MDA), PON1 activity and TSA concentration caused by the inflammatory and oxidative stress state caused by the infection can be used as new biomarkers for this infection.
{"title":"Evaluation of total sialic acid, paraoxonase activity and malondialdehyde in cows with subclinical paratuberculosis","authors":"E. Akyuz, A. Kükürt, M. Makav, E. Gökçe, H. Eroğlu, H. Deveci, G. Gökce, M. Karapehlivan","doi":"10.12681/jhvms.25689","DOIUrl":"https://doi.org/10.12681/jhvms.25689","url":null,"abstract":"In this study, it was aimed to evaluate total sialic acid (TSA), paraoxonase activity (PON1), malondialdehyde (MDA) and some serum biochemical parameters in cows with subclinical paratuberculosis. Paratuberculosis (PT) test was performed on 500 cows aged 2-6 years by ELISA method. Twenty-four cows diagnosed with paratuberculosis constituted the PT group and 12 healthy cows constituted the control group. According to the results, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and UREA levels were found to be statistically higher (p <0.05) in the group with paratuberculosis compared to the control group. Iron (Fe) levels were found to be lower than the control group (p> 0.05). Compared to the control group, there was a decrease in PON1 activity and a significant increase in TSA and MDA levels in the PT group (p <0.05). In conclusion, in subclinical paratuberculosis infection, the long incubation period and the absence of clinical findings until advanced stages constitute a handicap. However, our findings show that the determination of lipid peroxidation (MDA), PON1 activity and TSA concentration caused by the inflammatory and oxidative stress state caused by the infection can be used as new biomarkers for this infection.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46348429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
GH gene is one of the important genes that affect the productive and physiological traits in livestock. This study aimed to use a meta-analysis to determine the association of the GH gene with growth traits in cattle. Four different genetic models were used: dominant LL + LV versus VV, recessive LL versus LV + VV, complete over dominant LL+VV vs. LV and co-dominant LL vs. LV, LL vs. VV and LV vs. VV. The random-effect model was used in data analysis based on I2. Meta-analysis showed an effect of GH genotypes on the birth weight (BW) under the recessive (p<0.05) and complete over dominant (p<0.01) models. However, no association (p>0.05) was found under the dominant model. In terms of co-dominant model, the GH polymorphism showed a significant association with BW (SMD = 0.359, 95% CI = 0.119 to 0.599, p = 0.003) under LL vs. LV genotype combination patterns. No association was found between GH polymorphism and BW under LL vs. VV genotype combination. The results showed that the LL genotype had a significant effect on BW traits from LV and VV genotypes when comparing the models. The results showed an effect of the GH on ADG under four genetic models (p<0.05).
{"title":"A meta-analysis of the association between Growth Hormone (GH) gene polymorphism and growth traits in cattle breeds","authors":"M. Bayraktar, M. Özdemir","doi":"10.12681/jhvms.29407","DOIUrl":"https://doi.org/10.12681/jhvms.29407","url":null,"abstract":"GH gene is one of the important genes that affect the productive and physiological traits in livestock. This study aimed to use a meta-analysis to determine the association of the GH gene with growth traits in cattle. Four different genetic models were used: dominant LL + LV versus VV, recessive LL versus LV + VV, complete over dominant LL+VV vs. LV and co-dominant LL vs. LV, LL vs. VV and LV vs. VV. The random-effect model was used in data analysis based on I2. Meta-analysis showed an effect of GH genotypes on the birth weight (BW) under the recessive (p<0.05) and complete over dominant (p<0.01) models. However, no association (p>0.05) was found under the dominant model. In terms of co-dominant model, the GH polymorphism showed a significant association with BW (SMD = 0.359, 95% CI = 0.119 to 0.599, p = 0.003) under LL vs. LV genotype combination patterns. No association was found between GH polymorphism and BW under LL vs. VV genotype combination. The results showed that the LL genotype had a significant effect on BW traits from LV and VV genotypes when comparing the models. The results showed an effect of the GH on ADG under four genetic models (p<0.05).","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41717852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was performed to investigate the effect of dietary aspirin (A) on broilers. A completely randomized design using 120 one-day-old male broiler chicks Ross 308, three levels of aspirin (0, 50, and 100 mg/kg) was used in 4 replicates, each including 10 chicks, during 42 days. The effects of different levels of aspirin, added to a basal diet, on blood plasma parameters, liver enzymes, immune system, cecal microflora, and fatty acids profile of breast muscle of chicks were investigated. Data analysis was performed by SAS statistical software and the comparison of the means with Duncan's test. The results showed that the effects of aspirin on blood parameters, liver enzymes of broilers (except for alkaline phosphatase which significantly reduced), the humoral immune system in response to antigen injection as sheep red blood cell (SRBC), antibody titer against Newcastle and influenza virus were not significant (P≥0.05). The highest percentage of neutrophils and eosinophils was related to this treatment. Also, aspirin treatments increased numerically the levels of unsaturated fatty acids and decreased saturated fatty acids. Moreover, aspirin led to a reduction in the population of Escherichia coli. So, based on the results of the present study, the use of 100 mg/kg aspirin in the diet of broilers is recommendable.
{"title":"Effects of dietary aspirin supplementation on liver enzymes, immune response, cecum microflora and fatty acids profile in breast meat of broiler chickens","authors":"M. Tavakoli, M. Bouyeh, A. Seidavi","doi":"10.12681/jhvms.27695","DOIUrl":"https://doi.org/10.12681/jhvms.27695","url":null,"abstract":"The present study was performed to investigate the effect of dietary aspirin (A) on broilers. A completely randomized design using 120 one-day-old male broiler chicks Ross 308, three levels of aspirin (0, 50, and 100 mg/kg) was used in 4 replicates, each including 10 chicks, during 42 days. The effects of different levels of aspirin, added to a basal diet, on blood plasma parameters, liver enzymes, immune system, cecal microflora, and fatty acids profile of breast muscle of chicks were investigated. Data analysis was performed by SAS statistical software and the comparison of the means with Duncan's test. The results showed that the effects of aspirin on blood parameters, liver enzymes of broilers (except for alkaline phosphatase which significantly reduced), the humoral immune system in response to antigen injection as sheep red blood cell (SRBC), antibody titer against Newcastle and influenza virus were not significant (P≥0.05). The highest percentage of neutrophils and eosinophils was related to this treatment. Also, aspirin treatments increased numerically the levels of unsaturated fatty acids and decreased saturated fatty acids. Moreover, aspirin led to a reduction in the population of Escherichia coli. So, based on the results of the present study, the use of 100 mg/kg aspirin in the diet of broilers is recommendable.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46473088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Multidrug-resistant (MDR) Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) has increased markedly in recent years, which has currently posed a major challenge in antimicrobial treatments and raised concerns regarding possible transfer of such bacteria through the food chain. The aim of this research was to investigate the prevalence of ESBL-producing E. coli in milk, cheese and meat samples and to determine their virulence, pathotype, serotype, antibiotic resistance and genetic relatedness. A total of 300 food samples were purchased from public markets in different districts of Giresun city. Five (1.6%) of 300 food samples resulted positive for ESBL-producing E. coli isolation. β-Lactamase-encoding genes of the CTX-M (20%), TEM (40%), and SHV (20%) groups were detected singly or in combination. Five ESBL-producing E. coli isolates were further analysed for the presence of virulence genes. Virulence factor genes detected were hlyA (20%), ehlyA (20%), iutA (60%), iucD (40%), fimH (100%), kpsMTII (100%) and traT (100%). Of 130 E.coli isolates, 5 ESBL-producing E. coli strains (totally 3.8%) were isolated, including 3 EHEC (60%). No EIEC, ETEC, EPEC, DAEC or EAggEC3 was detected. In PCR, three stx2 (60%) and one flicH7 (20%) encoding genes were found in ESBL-producing E. coli isolates. In addition, genes encoding Shiga toxins were detected in 3 of 5 isolates, three isolates (60%) encoded O128 serotype.The antibiotic susceptibility test of positive isolates showed resistant to cefuroxime, trimethoprim/ sulfamethoxazole, cefazolin, streptomycin, ceftriaxone, tetracycline, ampicillin and trimethoprim. Most of ESBL-producing E. coli isolates showed 80% MDR phenotypes against at least four classes of antibiotics. Specific-PCR detection of antibiotic resistance genes showed the prevalence of the tetA gene in most of the isolates (80%), followed by dfrA, qnr, aadA1 and sul1. PFGE results show that the isolates from different districts presented no clonal relatedness. This is the first report of the characteristics of multidrug resistance ESBL-producing shigatoxigenic E. coli in dairy and meat products in a local city in Turkey. Our findings indicate that dairy and meat products could be reservoirs of MDR ESBL-producing E. coli strains that were possessed several virulence factors and may be a cause of concern for human health.
{"title":"Prevalence, Characterization and PFGE profiles of multidrug resistance extended spectrum β-lactamase producing Escherichia coli strains in animal-derived food products from public markets in Eastern Turkey","authors":"T. Cebeci","doi":"10.12681/jhvms.29251","DOIUrl":"https://doi.org/10.12681/jhvms.29251","url":null,"abstract":"Multidrug-resistant (MDR) Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) has increased markedly in recent years, which has currently posed a major challenge in antimicrobial treatments and raised concerns regarding possible transfer of such bacteria through the food chain. The aim of this research was to investigate the prevalence of ESBL-producing E. coli in milk, cheese and meat samples and to determine their virulence, pathotype, serotype, antibiotic resistance and genetic relatedness. A total of 300 food samples were purchased from public markets in different districts of Giresun city. Five (1.6%) of 300 food samples resulted positive for ESBL-producing E. coli isolation. β-Lactamase-encoding genes of the CTX-M (20%), TEM (40%), and SHV (20%) groups were detected singly or in combination. Five ESBL-producing E. coli isolates were further analysed for the presence of virulence genes. Virulence factor genes detected were hlyA (20%), ehlyA (20%), iutA (60%), iucD (40%), fimH (100%), kpsMTII (100%) and traT (100%). Of 130 E.coli isolates, 5 ESBL-producing E. coli strains (totally 3.8%) were isolated, including 3 EHEC (60%). No EIEC, ETEC, EPEC, DAEC or EAggEC3 was detected. In PCR, three stx2 (60%) and one flicH7 (20%) encoding genes were found in ESBL-producing E. coli isolates. In addition, genes encoding Shiga toxins were detected in 3 of 5 isolates, three isolates (60%) encoded O128 serotype.The antibiotic susceptibility test of positive isolates showed resistant to cefuroxime, trimethoprim/ sulfamethoxazole, cefazolin, streptomycin, ceftriaxone, tetracycline, ampicillin and trimethoprim. Most of ESBL-producing E. coli isolates showed 80% MDR phenotypes against at least four classes of antibiotics. Specific-PCR detection of antibiotic resistance genes showed the prevalence of the tetA gene in most of the isolates (80%), followed by dfrA, qnr, aadA1 and sul1. PFGE results show that the isolates from different districts presented no clonal relatedness. This is the first report of the characteristics of multidrug resistance ESBL-producing shigatoxigenic E. coli in dairy and meat products in a local city in Turkey. Our findings indicate that dairy and meat products could be reservoirs of MDR ESBL-producing E. coli strains that were possessed several virulence factors and may be a cause of concern for human health.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43818298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Global animal feeding strategies have been modified due to several ecosystem changes that cause stress and decline in health, growth, and yields. According to the literature, natural additives have been fundamental to animal health from day to day. The present study measured changes in antioxidant status, immunity, liver functions and organ weights tested under stress conditions to determine whether dietary supplementation with phytogenic additive could provide beneficial effects. Forty-eight adult, male Sprague-Dawley rats were randomly separated into four groups; Control(C), Stress(S), Treatment(Tr), Treatment and Stress(TrS). Rats in groups Tr and TrS received phytogenic additive by adding into water (2mL/L) 5days a week for 28days. All rats were exposed to prolonged light phase conditions (18h light: 6h dark) for 14 days. Also, two chronic stresses, isolation and crowded environments, were applied to animals in the Stress and TrS groups. There was a significant decline in the oxidant status in untreated stress group, while phytogenic additive fed rats maintained a significantly higher total antioxidant status. This study also showed a significant increase in IL-4 and decrease in IFN-γ in the untreated Stress group compared to the Control group. There were increases in liver enzymes in the Stress group in comparison to the Control group. After the phytogenic treatment, there was an increase in the weight of the liver, intestine, brain and testes. In conclusion, this study showed that supplementation of phytogenic additive containing milk thistle and artichoke with choline, carnitine, vitamin E and melatonin describes the protective effects on antioxidant status, immunological parameters and liver functions under mixed stress conditions.
{"title":"The influence of phytogenic additive on the antioxidant capacity, immunity and liver functions in stress-induced male rats","authors":"E. Koseli, N. Seyidoglu, R. Gurbanli, C. Aydin","doi":"10.12681/jhvms.27432","DOIUrl":"https://doi.org/10.12681/jhvms.27432","url":null,"abstract":"Global animal feeding strategies have been modified due to several ecosystem changes that cause stress and decline in health, growth, and yields. According to the literature, natural additives have been fundamental to animal health from day to day. The present study measured changes in antioxidant status, immunity, liver functions and organ weights tested under stress conditions to determine whether dietary supplementation with phytogenic additive could provide beneficial effects. Forty-eight adult, male Sprague-Dawley rats were randomly separated into four groups; Control(C), Stress(S), Treatment(Tr), Treatment and Stress(TrS). Rats in groups Tr and TrS received phytogenic additive by adding into water (2mL/L) 5days a week for 28days. All rats were exposed to prolonged light phase conditions (18h light: 6h dark) for 14 days. Also, two chronic stresses, isolation and crowded environments, were applied to animals in the Stress and TrS groups. There was a significant decline in the oxidant status in untreated stress group, while phytogenic additive fed rats maintained a significantly higher total antioxidant status. This study also showed a significant increase in IL-4 and decrease in IFN-γ in the untreated Stress group compared to the Control group. There were increases in liver enzymes in the Stress group in comparison to the Control group. After the phytogenic treatment, there was an increase in the weight of the liver, intestine, brain and testes. In conclusion, this study showed that supplementation of phytogenic additive containing milk thistle and artichoke with choline, carnitine, vitamin E and melatonin describes the protective effects on antioxidant status, immunological parameters and liver functions under mixed stress conditions.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46034150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Marami, A. Nobakht, Y. Mehmannavaz, F. Mazlum, S. Mahdavi
This experiment was performed to evaluate the effects of replacement of different levels of soybean meal with sunflower meal on laying performance and egg quality parameters in laying hens. In this experiment, 360 laying hens from the age of 47 to 57 weeks, in 9 treatments and 5 replications (8 hens in each replication) were divided into a 3 × 3 factorial arrangement consisting of sunflower meal (0, 30, and 60%) and enzyme additive (0, 100 and 200 g/ton) in a completely randomized design for ten weeks. The results showed that the replacement of soybean meal with sunflower meal and enzyme has significant effects on the performance of laying hens. So that 60% replacement of soybean meal with sunflower meal increased the amount of daily feed intake of laying hens (P<.0.05). The use of 200 gr/ton of multi enzyme in laying hens diet, without having significant effects on other performance parameters, increased the amount of daily feed intake (P<.0.05). Concurrently, there was a non-significant (P>.0.05) trend for egg quality parameters, but weights of egg albumin were affected significantly (P<.0.05) substitution of 60% of sunflower meal in diets increased the amount egg albumin weight. In general, in laying hens, it is possible to replace 60% of soybean meal with sunflower meal without adversely affecting the performance and egg quality. The use of a multi-enzyme only increased the amount of feed intake.
{"title":"Replacing of soybean meal with sunflower meal with and without multi-enzyme on laying performance and egg quality in Hy-Line laying hens","authors":"M. Marami, A. Nobakht, Y. Mehmannavaz, F. Mazlum, S. Mahdavi","doi":"10.12681/jhvms.27459","DOIUrl":"https://doi.org/10.12681/jhvms.27459","url":null,"abstract":"This experiment was performed to evaluate the effects of replacement of different levels of soybean meal with sunflower meal on laying performance and egg quality parameters in laying hens. In this experiment, 360 laying hens from the age of 47 to 57 weeks, in 9 treatments and 5 replications (8 hens in each replication) were divided into a 3 × 3 factorial arrangement consisting of sunflower meal (0, 30, and 60%) and enzyme additive (0, 100 and 200 g/ton) in a completely randomized design for ten weeks. The results showed that the replacement of soybean meal with sunflower meal and enzyme has significant effects on the performance of laying hens. So that 60% replacement of soybean meal with sunflower meal increased the amount of daily feed intake of laying hens (P<.0.05). The use of 200 gr/ton of multi enzyme in laying hens diet, without having significant effects on other performance parameters, increased the amount of daily feed intake (P<.0.05). Concurrently, there was a non-significant (P>.0.05) trend for egg quality parameters, but weights of egg albumin were affected significantly (P<.0.05) substitution of 60% of sunflower meal in diets increased the amount egg albumin weight. In general, in laying hens, it is possible to replace 60% of soybean meal with sunflower meal without adversely affecting the performance and egg quality. The use of a multi-enzyme only increased the amount of feed intake.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48154866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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