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Influences of dietary aspirin supplementation on growth performance, carcass characteristics and gastrointestinal organs of broilers 日粮补充阿司匹林对肉鸡生长性能、胴体性状及胃肠道器官的影响
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-27 DOI: 10.12681/jhvms.27696
M. Tavakoli, M. Bouyeh, A. Seidavi
This study was performed to investigate the effect of dietary aspirin (A) on some performance traits in broilers using a completely randomized design, 120 one-day-old male broiler chicks Ross 308, three levels of aspirin (0, 50, and 100 mg/kg), in 4 replicates, each including 10 chicks, during 42 days. The effects of different levels of aspirin, added to a basal diet, on performance, carcass characteristics, and digestive organs of chicks were investigated. Data analysis was performed by SAS statistical software and the comparison of the means with Duncan's test. The results showed that the chickens fed by a diet containing 100 mg/kg of aspirin had the highest feed intake and weight gain and the best feed conversion ratio compared to the other treatments. Also, the lowest production cost and the best European factor were related to treatment A100 (P<0.05). In addition, the use of the same level of aspirin resulted in a significant increase in some carcass properties and a decrease in ventricular fat compared to the control (P<0.05). So, based on the results of the present study, the use of 100 mg/kg aspirin in the diet of broilers is recommendable to improve some performance parameters.
本研究采用完全随机设计,研究了日粮中阿司匹林(A)对肉鸡某些性能性状的影响,120只一日龄雄性肉鸡Ross 308,三种水平的阿司匹林(0、50和100mg/kg),在42天的4个重复中,每个重复包括10只鸡。研究了在基础日粮中添加不同水平的阿司匹林对雏鸡生产性能、胴体特性和消化器官的影响。数据分析采用SAS统计软件进行,并将平均值与Duncan检验进行比较。结果表明,与其他处理相比,含100mg/kg阿司匹林的日粮喂养的鸡具有最高的采食量和体重增加,并且具有最佳的饲料转化率。此外,最低的生产成本和最佳的欧洲因素与处理A100有关(P<0.05)。此外,与对照组相比,使用相同水平的阿司匹林显著提高了胴体的某些性能,降低了心室脂肪(P<0.05),因此,基于本研究的结果,在肉鸡日粮中使用100mg/kg阿司匹林可改善肉鸡的某些性能参数。
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引用次数: 0
Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows 荷斯坦奶牛和瑞士棕色奶牛MASP2、TG5和DQA1基因的遗传多态性
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.29524
A. El Zeer, M. Fouda, R. Darwish, A. Ateya
The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.
本研究的目的是使用PCR-DNA测序技术在70头荷斯坦奶牛和棕色瑞士奶牛(每头35头)中研究MASP2、TG5和DQA1基因的多态性。在完全无菌条件下,将每只动物的血样收集到含有EDTA二钠作为抗凝剂的试管中,用于DNA提取。PCR扩增了305bp的MASP2、545bp的TG5和373bp的DQA1基因。DNA测序评估详细阐述了两个品种之间单核苷酸多态性(SNPs)形式的核苷酸序列变异。在荷斯坦品种的研究基因中鉴定出的SNPs为MASP2基因中的A46G和TG5基因中的C371T。在DQA1基因中,还报道了棕色瑞士品种特有的T53C SNP。卡方检验显示,所有已鉴定的SNPs的分布都存在显著差异(P<0.001)。因此,已鉴定的单核苷酸多态性可作为荷斯坦和布朗-瑞士品种有效鉴定的候选者和参考指南;因此,开发一种用于奶牛品种生产和免疫特性的标记辅助选择(MAS)。
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引用次数: 0
Determination of predictive factors for the clinical cure rate of endometritis in Holstein dairy cows 荷斯坦奶牛子宫内膜炎临床治愈率预测因素的测定
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.28687
A. Hajibemani, A. Mirzaei
Endometritis is one of the most common disorders that negatively influence reproductive performance in dairy cows. The purpose of this study was to determine the predictive factors of the clinical cure of endometritis. A total of 700 Holstein dairy cows were examined for signs of clinical endometritis between 30 and 40 days in milk (the first examination in the time of clean test). A total of 131 cows with clinical endometritis were assigned for treatment. Cytological examination, reagent test strips, and characteristics of reproductive organs were evaluated at the time of treatment (the first examination) and two weeks after the treatment (the second examination: for evaluation of response to treatment of endometritis). After evaluation of treatment, cows were grouped into the responsive (n=56) and unresponsive (n=75) to treatment. Percentages of non-degenerated neutrophil (NDN), degenerated neutrophil (DN), total neutrophils and leukocyte esterase activity were significantly higher (P<0.05) in the unresponsive cows than those of responsive ones to treatment at the second examinations. Moreover, the nitrate test was significantly higher in the unresponsive group than the responsive group at the first examination. However, protein concentration and percentages of lymphocyte cells were significantly higher in the unresponsive animals than the responsive cows at the first and second stages of examination (P<0.05). Increasing of lymphocytes percentage, protein concentration, and nitrate test at the first stage of examination in the unresponsive cows showed more sever of inflammatory conditions and uterine infection in the unresponsive cows. Evaluation of these factors can predict the clinical cure of endometritis in dairy cows.
子宫内膜炎是影响奶牛繁殖性能的最常见疾病之一。本研究的目的是确定子宫内膜炎临床治愈的预测因素。对700头荷斯坦奶牛在30至40天的牛奶中进行了临床子宫内膜炎症状检查(清洁试验时的第一次检查)。共有131头患有临床子宫内膜炎的奶牛被分配接受治疗。在治疗时(第一次检查)和治疗后两周(第二次检查:评估对子宫内膜炎治疗的反应)评估细胞学检查、试剂试纸条和生殖器官特征。在评估治疗后,将奶牛分为对治疗有反应(n=56)和无反应(n=75)。在第二次检查中,对治疗无反应的奶牛的非变性中性粒细胞(NDN)、变性中性粒细胞核(DN)、总中性粒细胞和白细胞酯酶活性的百分比显著高于对治疗有反应的奶牛(P<0.05)。此外,在第一次检查时,无反应组的硝酸盐测试显著高于有反应组。然而,在第一和第二阶段的检查中,无反应动物的蛋白质浓度和淋巴细胞百分比显著高于有反应的奶牛(P<0.05),无反应奶牛在第一阶段的硝酸盐测试显示,无反应奶牛的炎症状况和子宫感染更严重。评价这些因素可以预测奶牛子宫内膜炎的临床治愈率。
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引用次数: 0
Distribution of Salmonella virulence factors originated from sheep and cattle in Algerian slaughterhouses 产自阿尔及利亚屠宰场羊和牛的沙门氏菌毒力因子分布
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.29148
S. Nouichi, L. Mezali, TM Hamdi
The present study was carried out to determine the virulence gene profiles associated with Salmonella subsp. enterica strains recovered from cattle and sheep samples by polymerase chain reaction (PCR). A total of 84 Salmonella isolates belonging to 10 serovars were screened for presence of 8 different virulence genes (invA, pefA, sefA, pipB, sseC, ssaP, spvC and iroB) using their specific primers. Different distribution patterns of these genes were noticed amongst the isolates. While the iroB gene was the most identified (n= 65, 77.4%), the sefA gene was not detected in all tested strains. pefA and spvC genes were detected in S. Typhimurium only with a rate of 3.6%. The four other genes were found to be present in 61.9% each. Occurrence of virulence genes did not vary significantly (P> 0.05) by serovar or animal species, neither by type of sample. The presence of all screened genes was significantly associated with resistance to at least one antibiotic from different classes except quinolones. These results show that transmissible pathogenic Salmonella strains are circulating in slaughterhouses in Algeria.
本研究旨在确定与沙门氏菌亚种相关的毒力基因谱。采用聚合酶链式反应(PCR)从牛和羊样品中检出肠炎菌株。利用特异性引物对10个血清型84株沙门氏菌进行了8种不同毒力基因(invA、pefA、sefA、pipB、sseC、ssaP、spvC和iroB)的筛选。这些基因在不同的分离株中有不同的分布模式。iroB基因检出最多(n= 65, 77.4%), sefA基因未检出。鼠伤寒沙门氏菌中pefA和spvC基因检出率仅为3.6%。其他四种基因各占61.9%。毒力基因的发生在不同的血清型和动物种类之间无显著差异(P> 0.05),在不同的样品类型之间无显著差异。除喹诺酮类药物外,所有筛选基因的存在与对至少一种不同类别抗生素的耐药性显著相关。这些结果表明,传染性致病性沙门氏菌菌株在阿尔及利亚的屠宰场中流行。
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引用次数: 0
Border Disease In A Lamb: A Case Report 羔羊边境疾病一例报告
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.28216
F. Akkaya, FZ Evci, ZA Küpeli, Ç. Kandemir, T. Taşkin, N. Koşum
In this case, it was aimed to report the clinical, hematological and pathological analysis results of a lamb born as infected with border disease virus (BDV). Severe central nervous system findings such as standing inability, continuous teeth grinding, muscle fasciculations in the head, ear and tail, hair loss around the eyes, lips and ears, abnormal appearance of the fleece, ataxia/rhythmic tremor and incoordination were detected in the case of lamb infected with BDV. The presence of BDV antibody and the levels of Triiodothyronine (T3) and Thyroxine (T4) were investigated in the serum samples for definite diagnosis. The tissue samples obtained from the brain and cerebellum were evaluated histopathologically and diagnosis of BDV was confirmed by necroscopic findings.  
在本病例中,目的是报告出生时感染边境病病毒(BDV)的羔羊的临床、血液学和病理分析结果。在感染BDV的羔羊中发现了严重的中枢神经系统症状,如站立能力丧失、连续磨牙、头、耳和尾肌肉束动、眼睛、嘴唇和耳朵周围毛发脱落、羊毛外观异常、共济失调/节律性震颤和不协调。检测血清BDV抗体及三碘甲状腺原氨酸(T3)、甲状腺素(T4)水平,以明确诊断。从大脑和小脑获得的组织样本进行组织病理学评估,并通过坏死镜检查证实BDV的诊断。
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引用次数: 0
Pre- and post -exercise variation of blood parameters on performance endurance horses: a first race study from Algeria 高性能耐力马运动前后血液参数的变化:来自阿尔及利亚的首次比赛研究
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.28752
M. Chikhaoui, S. Fadhéla, Cza Belalia, N. Ayad
Abstract: This paper presents the first study conducted in Algeria to determine the pre and post-race hematological and biochemical parameters of horses of a 90 km endurance race for eliminated horses’ category and those who completed the races with good performance. This study was conducted on 15 endurance horses (10 Arabian Thoroughbred, 4 Arabian-Barb and 1 Barb). Nine (9) horses successfully completed the endurance race, whereas the other 6 were eliminated. Blood samples were collected from each animal before and after the end of the race. Whole blood samples were used for measuring the blood-biochemical parameters.  Our results showed a significant increase in the hematological parameters (RBC, HB, Ht and MCV), the total leucocyte count, neutrophils and platelets for the group of horses who finished the race successfully. A significant decrease was noticed for the lymphocytes and eosinophils numbers while serum biochemical parameters showed a significant increase of Ca, TG, urea, U-A, AST and CK and a decrease of Mg, CHE, GLU and ƔGT. This study showed that, for the eliminated horses, there were significant changes in values of the total leucocytic count, lymphocytes, neutrophils, HB and Ht as compared to good performance horses. Serum biochemical parameters showed a significant increase of TG, U-A, AST and CK and a significant decrease of Ca, Mg, CHE and ƔGT. Most of the hemato-biochemical parameters of both the eliminated horses and those who completed the race successfully depicted significant differences. The results of this study suggested that biochemical findings could help identify horses with an increased risk of developing metabolic alterations before their elimination during competitive endurance races of different distances. Therefore, proper management protocols, standard feeding and watering with proper preparation of animals before the race could prevent electrolytes imbalance and pathological affections during racing.
摘要:本文首次在阿尔及利亚进行了一项研究,测定了90公里耐力赛中被淘汰的马和表现良好的马的赛前和赛后血液学和生化参数。本研究以15匹耐力马(10匹阿拉伯纯种马、4匹阿拉伯刺马和1匹刺马)为研究对象。9匹马成功地完成了耐力赛,而其他6匹马则被淘汰。在比赛结束前后,从每只动物身上采集了血液样本。采用全血标本测定血液生化指标。我们的结果显示,成功完成比赛的马的血液学参数(RBC, HB, Ht和MCV),白细胞总数,中性粒细胞和血小板显著增加。淋巴细胞和嗜酸性粒细胞显著减少,血清生化指标Ca、TG、尿素、U-A、AST和CK显著升高,Mg、CHE、GLU和ƔGT显著降低。本研究表明,与表现良好的马相比,被淘汰的马的总白细胞计数、淋巴细胞、中性粒细胞、HB和Ht值发生了显著变化。血清生化指标TG、U-A、AST、CK显著升高,Ca、Mg、CHE、ƔGT显著降低。被淘汰的马和成功完成比赛的马的大部分血液生化参数都有显著差异。这项研究的结果表明,生化研究结果可以帮助识别在不同距离的竞争性耐力比赛中被淘汰之前发生代谢改变风险增加的马。因此,在赛前适当的管理方案、规范的喂养和饮水以及适当的动物准备,可以防止比赛中电解质失衡和病理影响。
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引用次数: 0
Determination of Direct and Indirect Effects on Milk Yield of Anatolian Buffaloes Using Path Analysis 用通径分析法测定对安纳托利亚水牛产奶量的直接和间接影响
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.28470
A. Şahin, Y. Aksoy, A. Yildirim, Z. Ulutaş
This study aimed to determine the direct and indirect effects of the independent variables presented by the lactation lenght (LL), age of calving (CAGE), and daily milk yield (DMY) on the dependent variable of lactation milk yield (LMY) in Anatolian buffaloes. In this study, a total of 3761 LMY records of the 834 Anatolian buffaloes calving between 2012 and 2017 in Tokat province and around were used as the research material. In the study, the simple correlation coefficients between the dependent variable of LMY and independent variables were determined positive and significant (P<0.001). The research specified the direct determination coefficients of the independent variables of the LL, CAGE, and DMY on the dependent variable of LMY as 0.378, 0.004, and 0.350, respectively. The path coefficients related to LL, CAGE, and DMY were determined as 0.615, 0.021, and 0.592, respectively. Since the DMY and LL examined in the study had a high direct impact on the lactation milk yield, therefore it could be suggested that using these data as criteria in breeding programs conducted in the herds evaluated in the study will increase the success in the selections applied.
本研究旨在确定安纳托利亚水牛泌乳长度(LL)、产仔年龄(CAGE)和日产奶量(DMY)等自变量对泌乳产奶量(LMY)因变量的直接和间接影响。在本研究中,共使用了3761份2012年至2017年间在托卡特省及周边地区产仔的安纳托利亚水牛的LMY记录作为研究材料。研究中,LMY的因变量与自变量之间的简单相关系数为正且显著(P<0.001)。研究表明,LL、CAGE和DMY的自变量对LMY因变量的直接决定系数分别为0.378、0.004和0.350。与LL、CAGE和DMY相关的通径系数分别为0.615、0.021和0.592。由于研究中检查的DMY和LL对泌乳乳产量有很高的直接影响,因此可以建议,在研究中评估的畜群中进行的育种计划中使用这些数据作为标准,将提高应用选择的成功率。
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引用次数: 0
Rupture of the Prepubic Tendon in a Quadruplet Pregnant Romanov Ewe 四胞胎孕妇Romanov Ewe的耻骨前肌腱断裂
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.28261
Ö. Yılmaz, Ö. Biçici, E. Karakaya-Bilen
 A case of prepubic tendon rupture in a Romanov ewe in which four live lambs were removed by cesarean section is presented. A three-year-old ewe, on the 142±3 days of the second gestation was brought to the Siirt University Animal Health Application and Research Center with a sudden downward expansion of the abdomen during the last one day.  When ewe was examined reluctance to locomotion, mild depression, tachycardia, tachypnea and abdominal peculiar enlargement on the left side more than right side were noticed. Because of this case was detected early the general condition was not so bad and clinical examination revealed that swelling was non-painful and non-edematous. Ultrasonography was performed to detect viability of the fetuses, and to eliminate possibility of hernia of the abdominal muscles. Based on anamnesis, abdominal palpation, and clinical examination, this case was diagnosed with prepubic tendon rupture. The prognosis of saving the lives of ewe and fetuses depends on successfully handling all aspects of the case in the last period of pregnancy. Although it is not possible to repair the abdominal integrity surgically in cases of prepubic tendon rupture in the ewe, a planned cesarean operation is indicated to increase the survival rates of both ewe and lambs. 
报道了一例Romanov母羊的耻骨前肌腱断裂,其中四只活羊羔通过剖宫产取出。一只三岁的母羊在第二次妊娠142±3天被带到希尔特大学动物健康应用与研究中心,在最后一天腹部突然向下扩张。当检查母羊的不情愿运动时,注意到左侧的轻度抑郁、心动过速、呼吸急促和腹部异常肿大多于右侧。因为这个病例很早就被发现了,所以总体情况并没有那么糟糕,临床检查显示肿胀没有疼痛和水肿。超声检查是为了检测胎儿的生存能力,并消除腹肌疝的可能性。根据记忆、腹部触诊和临床检查,本病例被诊断为耻骨前肌腱断裂。能否挽救母羊和胎儿的生命,取决于能否在妊娠最后阶段成功处理好病例的各个方面。尽管在母羊胸骨前肌腱断裂的情况下,不可能通过手术修复腹部完整性,但有计划的剖宫产手术可以提高母羊和羔羊的存活率。
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引用次数: 0
The possibilities of using quinoa flour in the production of chicken meat patties 藜麦粉用于鸡肉肉饼生产的可能性
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.29784
R. Meral, N. Kutlu, A. Alav, O. Kılınççeker
In this study, the mixtures obtained by mixing quinoa flour with wheat flour in different proportions were added to chicken meat patties and their effects on some quality characteristics were investigated. The yields of the meatballs prepared with the mixes containing 50% and 100% quinoa flour were higher than those of other meatballs (69.59% and 69.71%, respectively). The moisture retention of the fried meatballs prepared with mixtures containing 50%, 70% and 100% quinoa flour was found to be 45.80%, 45.97% and 51.09%, respectively. The results indicated that the moisture retention of these meatballs was higher than those of meatballs containing 30 and 0% quinoa flour. In contrast, oil absorption rates in the fried samples were in the range of 4.46-5.65% for all quinoa-containing samples and were lower compared to the control sample. Firmness decreased in meat patties prepared with mixtures containing high quinoa rates. It was observed that quinoa flour did not have a negative effect on quality factors. It was concluded that especially the mixtures containing 30% and 50% quinoa flour can be recommended.
在本研究中,将藜麦粉和小麦粉以不同比例混合得到的混合物加入鸡肉肉饼中,并研究了它们对一些质量特性的影响。用含有50%和100%藜麦粉的混合物制备的肉丸的产量高于其他肉丸(分别为69.59%和69.71%)。用含有50%、70%和100%藜麦粉的混合物制备的油炸肉丸的保水率分别为45.80%、45.97%和51.09%。结果表明,这些丸子的保湿性高于含有30%和0%藜麦粉的丸子。相反,对于所有含有藜麦的样品,油炸样品中的吸油率在4.46-5.65%的范围内,并且与对照样品相比更低。用含有高藜麦含量的混合物制备的肉饼硬度降低。据观察,藜麦粉对品质因素没有负面影响。得出的结论是,尤其是含有30%和50%藜麦粉的混合物是可以推荐的。
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引用次数: 0
Antimicrobial and antibiofilm forming activity of Origanum munzurense against some Gram-Positive bacteria and yeast 土豆泥对革兰氏阳性菌和酵母菌的抑菌活性和抗膜活性
IF 0.4 4区 农林科学 Q4 Veterinary Pub Date : 2023-01-25 DOI: 10.12681/jhvms.28910
E. Zeybek, A. Kart, H. Yalçin
In vitro antimicrobial and antibiofilm forming activities of Origanum munzurense extract were investigated against some gram-positive bacteria and yeast strains. Liquid microdilution method and microplate method were used for antimicrobial and antibiofilm forming activities, respectively. Minimal inhibitory concentration values were found to be 2 mg/ml for Enterococcus faecalis ATCC 29212, 64 mg/ml for Listeria monocytogenes RSKK 02028, 2 mg/ml for L. monocytogenes RSKK 472, 2 mg/ml for Bacillus cereus NRRL 569 , 128 mg/ml for S. aureus ATCC 25923, 32 mg/ml for S. aureus (MRSA) ATCC 43300 strain, 64 mg/ml for Candida tropicalis ATCC 13803, 2 mg/ml for C. albicans ATCC 90028, 1 mg/ml for S. aureus FRI 918 and 1 mg/ml for Staphylococcus epidermidis ATCC 12228 strain. The extract prevented the biofilm formations of E. faecalis ATCC 29212, S. aureus FRI 918 and S.epidermidis ATCC 12228 at concentrations of 2 mg/ml, 500 µg/ml and 250 µg/ml, respectively. No biofilm formation was observed for the other bacteria tested.As a result, Origanum munzurense extract has antibacterial and antibiofilm activity on the selected pathogenic microorganisms tested. Due to these properties of O. munzurense, it could be considered as a plant-based antimicrobial agent to be used especially in the food industry.
研究了牛至提取物对部分革兰氏阳性菌和酵母的体外抗菌和成膜活性。液体微量稀释法和微板法分别用于抗菌和抗菌成膜活性。发现对粪肠球菌ATCC 29212的最小抑制浓度值为2 mg/ml,对单核细胞增生性李斯特菌RSKK 02028为64 mg/ml,单核细胞增多性李斯特菌RS KK 472为2 mg/ml,对蜡样芽孢杆菌NRRL 569为2 g/ml,对金黄色葡萄球菌ATCC 25923为128 mg/ml,金黄色葡萄球菌FRI 918为1mg/ml,表皮葡萄球菌ATCC 12228菌株为1mg/ml。提取物分别在2 mg/ml、500µg/ml和250µg/ml的浓度下阻止了粪肠球菌ATCC 29212、金黄色葡萄球菌FRI 918和表皮葡萄球菌ATCC 12228的生物膜形成。对于测试的其他细菌,没有观察到生物膜的形成。结果表明,牛至提取物对所选病原微生物具有抗菌和抗生物膜活性。由于O.munzurense的这些特性,它可以被认为是一种植物性抗菌剂,特别是在食品工业中使用。
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引用次数: 0
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Journal of the Hellenic Veterinary Medical Society
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