D. Kocatepe, H. Turan, B. Köstekli̇, C. Altan, B. Corapci
In this study, it was aimed to compare of nutritional composition, cholesterol, trans fatty acids, fatty acids, lipid quality index, vitamins (A, B9, B2, B12, D3), selenium (Se), and iron (Fe) contents between farmed Atlantic salmon and rainbow trout. Crude protein, crude fat, carbohydrate, and energy values of Atlantic salmon were slightly higher than rainbow trout while crude ash and moisture values were higher in rainbow trout (p < 0.05). The cholesterol content of samples in rainbow trout and Atlantic salmon were found as 77.37±1.07 mg 100g-1 and 99.45±2.72 mg 100g-1, respectively (p<0.05). Trans fatty acids of both species were found as <0.3 g 100g-1. ƩSFA value of rainbow trout was found higher than the Atlantic salmon, on the other hand, the ƩMUFA values were found higher in the Atlantic salmon (p<0.05). However, the ƩPUFA values were found similar for both fish species (p>0.05). In general, the results show that rainbow trout contains two times more EPA+DHA amount than Atlantic salmon. While vitamin A has been found higher in rainbow trout, other vitamins (B9, B2, B12, D3) have been higher in Atlantic salmon (p<0.05). Additionally, iron (Fe) and selenium (Se) contents in rainbow trout has been significantly higher than Atlantic salmon (p<0.05).TRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//
{"title":"Preliminary investigation of the nutritional composition of two commercial fish species: Rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)","authors":"D. Kocatepe, H. Turan, B. Köstekli̇, C. Altan, B. Corapci","doi":"10.12681/jhvms.27821","DOIUrl":"https://doi.org/10.12681/jhvms.27821","url":null,"abstract":"In this study, it was aimed to compare of nutritional composition, cholesterol, trans fatty acids, fatty acids, lipid quality index, vitamins (A, B9, B2, B12, D3), selenium (Se), and iron (Fe) contents between farmed Atlantic salmon and rainbow trout. Crude protein, crude fat, carbohydrate, and energy values of Atlantic salmon were slightly higher than rainbow trout while crude ash and moisture values were higher in rainbow trout (p < 0.05). The cholesterol content of samples in rainbow trout and Atlantic salmon were found as 77.37±1.07 mg 100g-1 and 99.45±2.72 mg 100g-1, respectively (p<0.05). Trans fatty acids of both species were found as <0.3 g 100g-1. ƩSFA value of rainbow trout was found higher than the Atlantic salmon, on the other hand, the ƩMUFA values were found higher in the Atlantic salmon (p<0.05). However, the ƩPUFA values were found similar for both fish species (p>0.05). In general, the results show that rainbow trout contains two times more EPA+DHA amount than Atlantic salmon. While vitamin A has been found higher in rainbow trout, other vitamins (B9, B2, B12, D3) have been higher in Atlantic salmon (p<0.05). Additionally, iron (Fe) and selenium (Se) contents in rainbow trout has been significantly higher than Atlantic salmon (p<0.05).TRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47653954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Aim: The aim of the study was to investigate the applicability of an up-to-date method, sperm selection with nanoparticles at different temperatures (37ºC, 21ºC, and 4ºC), on Angora buck semen. Material and Methods: Semen samples were collected from 3 Angora bucks with 7 repetitions and each sample was diluted and divided into 4 groups. Nanopurification procedure was applied by using a nanoparticle dose of 10 μg/ml to the three groups at 37ºC, 21ºC, and 4ºC while in the control group samples were cryopreserved without nanopurificiation treatment. Post-thaw motility and morphology parameters of the sperm samples were analyzed by using computer-aided sperm analysis (CASA) and fluorescein microscopy.Results: After nanopurification at 37ºC total motility, linearity (LIN) (P<0.05), curvilinear velocity (VCL), straight-line velocity (VSL), and acrosome integrity parameters (P<0.001) were higher than the control group while the semen samples nanopurified at 21ºC were superior to the control group in terms of LIN, straightness (STR) (P<0.05), VCL, VSL, average path velocity (VAP), wobble and acrosome integrity (P<0.001). There was no significant difference between the control and the 4ºC group.Conclusion: In conclusion, nanopurification procedure on buck semen was useful in terms of parameters that affect fertility and the method can be performed at 21ºC, as well as 37ºC, to adapt the procedure to field conditions;
{"title":"A novel approach to sperm selection: Nanoparticle-based purification improves quality of Angora cryopreserved buck’s semen","authors":"H. Alemdar, MB Tırpan","doi":"10.12681/jhvms.28206","DOIUrl":"https://doi.org/10.12681/jhvms.28206","url":null,"abstract":"The Aim: The aim of the study was to investigate the applicability of an up-to-date method, sperm selection with nanoparticles at different temperatures (37ºC, 21ºC, and 4ºC), on Angora buck semen. Material and Methods: Semen samples were collected from 3 Angora bucks with 7 repetitions and each sample was diluted and divided into 4 groups. Nanopurification procedure was applied by using a nanoparticle dose of 10 μg/ml to the three groups at 37ºC, 21ºC, and 4ºC while in the control group samples were cryopreserved without nanopurificiation treatment. Post-thaw motility and morphology parameters of the sperm samples were analyzed by using computer-aided sperm analysis (CASA) and fluorescein microscopy.Results: After nanopurification at 37ºC total motility, linearity (LIN) (P<0.05), curvilinear velocity (VCL), straight-line velocity (VSL), and acrosome integrity parameters (P<0.001) were higher than the control group while the semen samples nanopurified at 21ºC were superior to the control group in terms of LIN, straightness (STR) (P<0.05), VCL, VSL, average path velocity (VAP), wobble and acrosome integrity (P<0.001). There was no significant difference between the control and the 4ºC group.Conclusion: In conclusion, nanopurification procedure on buck semen was useful in terms of parameters that affect fertility and the method can be performed at 21ºC, as well as 37ºC, to adapt the procedure to field conditions;","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46084135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It was aimed to investigate the genotypic structures of the Pit-1 gene from genomic DNA samples taken from Simmental and Brown Swiss breeds and determine the distribution of genotype and allele frequencies of bovine breeds and to determine the relationships between the detected genotypes and some performance traits. Pit-1/HinfI gene polymorphisms were determined using the PCR-RFLP method from genomic DNA obtained from Brown Swiss and Simmental breed cattle used in the study. According to the Hardy-Weinberg genetic test, the distribution of genotype frequencies of the studied population was found to be in equilibrium (p˃0.05) in both breeds. Genotype frequencies of AA, AB, and BB alleles of the Pit-1 gene were determined as 5.73%, 36.42%, and 57.85%, respectively, for Simmental breed, while the genotype frequencies for Brown Swiss breed were determined as 9.43%, 42.86%, and 48.01%, respectively. The actual milk yield for Simmental breed was determined as 5953 ± 364.2, 5212 ± 993.4, 5507 ± 889.5 kg, the 305-day milk yield as 5953 ± 276.8, 5642 ± 782.7, 5427 ± 246.7 kg, the lactation period as 307 ± 47, 293 ± 29, 303 ± 22 days, the daily milk yield as 19.53 ± 6, 18.49 ± 8, 17.81 ± 2 kg for AA, AB, and BB genotypes, respectively. The actual milk yield for the Brown Swiss breed was determined as 3606 ± 253, 3558 ± 530, 3999 ± 099 kg, the 305-day milk yield as 4920 ± 217, 4462 ± 900, 4635 ± 870 kg, the lactation period as 222 ± 36, 240 ± 04, 258 ± 37 days, the daily milk yield as 17.62 ± 2, 15.49 ± 2, 16.04 ± 3 kg for AA, AB, and BB genotypes, respectively. In the statistical analyses, the relationships between the performance traits studied and Pit-1 genotypes in Simmental and Brown Swiss cattle were not prove to be significant statistically.
{"title":"The relationships between Pit-1 gene polymorphism and some performance traits in simmental and brown swiss breeds","authors":"O. Toyğar, M. Ozdemir","doi":"10.12681/jhvms.28126","DOIUrl":"https://doi.org/10.12681/jhvms.28126","url":null,"abstract":"It was aimed to investigate the genotypic structures of the Pit-1 gene from genomic DNA samples taken from Simmental and Brown Swiss breeds and determine the distribution of genotype and allele frequencies of bovine breeds and to determine the relationships between the detected genotypes and some performance traits. Pit-1/HinfI gene polymorphisms were determined using the PCR-RFLP method from genomic DNA obtained from Brown Swiss and Simmental breed cattle used in the study. According to the Hardy-Weinberg genetic test, the distribution of genotype frequencies of the studied population was found to be in equilibrium (p˃0.05) in both breeds. Genotype frequencies of AA, AB, and BB alleles of the Pit-1 gene were determined as 5.73%, 36.42%, and 57.85%, respectively, for Simmental breed, while the genotype frequencies for Brown Swiss breed were determined as 9.43%, 42.86%, and 48.01%, respectively. The actual milk yield for Simmental breed was determined as 5953 ± 364.2, 5212 ± 993.4, 5507 ± 889.5 kg, the 305-day milk yield as 5953 ± 276.8, 5642 ± 782.7, 5427 ± 246.7 kg, the lactation period as 307 ± 47, 293 ± 29, 303 ± 22 days, the daily milk yield as 19.53 ± 6, 18.49 ± 8, 17.81 ± 2 kg for AA, AB, and BB genotypes, respectively. The actual milk yield for the Brown Swiss breed was determined as 3606 ± 253, 3558 ± 530, 3999 ± 099 kg, the 305-day milk yield as 4920 ± 217, 4462 ± 900, 4635 ± 870 kg, the lactation period as 222 ± 36, 240 ± 04, 258 ± 37 days, the daily milk yield as 17.62 ± 2, 15.49 ± 2, 16.04 ± 3 kg for AA, AB, and BB genotypes, respectively. In the statistical analyses, the relationships between the performance traits studied and Pit-1 genotypes in Simmental and Brown Swiss cattle were not prove to be significant statistically.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44169628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Özkan, U. Kaya, I. Karaaslan, S. Dalkiran, B. Camdeviren, M. Yüksel, A. Yakan
Weaning stress is crucial threatening factor in animal breeding. The aim of this study was to investigate the effects of weaning to oxidative stress and stress status of goat kids at the molecular levels. In the study, blood samples were collected at the weaning process (7 days before weaning- Pre-W, Day of weaning- Day-W and 7 days after weaning- Post-W) from 24 healthy Damascus goats. Cortisol, MDA (Malondialdehyde), COX-2 (Cyclooxygenase-2), and NRF2 (Nuclear factor erythroid 2-related factor 2) proteins levels were investigated in plasma, while COX-2 and NRF2 genes expression levels were determined from leukocytes. Compared to Pre-W, COX-2 gene was upregulated almost 2-fold in Day-W. On the other hand, NRF2 gene expression levels were tended to upregulation in Day-W, and it was upregulated approximately 2-fold in Post-W. While MDA levels were gradually increased in the weaning process, the levels of COX-2 and NRF2 proteins showed similar changes with gene expression trends. Positive correlations were also found between cortisol and COX-2 and NRF-2 protein levels in plasma. It was thought that significant outputs gained in terms of MDA levels in plasma of kids at the weaning process. For the first time, determination of COX-2 and NRF2 levels both mRNA and protein levels in the weaning process of goat kids were satisfactorily evaluated in this study. Particularly, NRF2 was found to have the potential to be a fundamental biomarker to assess the oxidative status of weaning kids.
{"title":"NRF2 is a potential biomarker for the evaluation of weaning-related oxidative stress in goat kids","authors":"H. Özkan, U. Kaya, I. Karaaslan, S. Dalkiran, B. Camdeviren, M. Yüksel, A. Yakan","doi":"10.12681/jhvms.28455","DOIUrl":"https://doi.org/10.12681/jhvms.28455","url":null,"abstract":"Weaning stress is crucial threatening factor in animal breeding. The aim of this study was to investigate the effects of weaning to oxidative stress and stress status of goat kids at the molecular levels. In the study, blood samples were collected at the weaning process (7 days before weaning- Pre-W, Day of weaning- Day-W and 7 days after weaning- Post-W) from 24 healthy Damascus goats. Cortisol, MDA (Malondialdehyde), COX-2 (Cyclooxygenase-2), and NRF2 (Nuclear factor erythroid 2-related factor 2) proteins levels were investigated in plasma, while COX-2 and NRF2 genes expression levels were determined from leukocytes. Compared to Pre-W, COX-2 gene was upregulated almost 2-fold in Day-W. On the other hand, NRF2 gene expression levels were tended to upregulation in Day-W, and it was upregulated approximately 2-fold in Post-W. While MDA levels were gradually increased in the weaning process, the levels of COX-2 and NRF2 proteins showed similar changes with gene expression trends. Positive correlations were also found between cortisol and COX-2 and NRF-2 protein levels in plasma. It was thought that significant outputs gained in terms of MDA levels in plasma of kids at the weaning process. For the first time, determination of COX-2 and NRF2 levels both mRNA and protein levels in the weaning process of goat kids were satisfactorily evaluated in this study. Particularly, NRF2 was found to have the potential to be a fundamental biomarker to assess the oxidative status of weaning kids.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45149161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to determine the effects of a trace element solution applied to cows in the transition period on the levels of fatty acids in the transition period. The material of this study consisted of 20 clinically healthy 3-5 year old simental cows kept under the same care and feeding conditions. Pregnant cows used in the study were divided into 2 equal groups with 10 cows in each group. A single dose of 20 ml of a trace element solution was administered intramuscularly to the pregnant cows in the study group 3 weeks before the birth. Blood samples were taken for analysis at the time of trace element administration, the moment of birth and 3 weeks after birth. Serum samples were analyzed using gas chromatography-mass spectrophotometry (GC-MS) device. When the findings of the study were examined, the fatty acid levels determined 3 weeks before the birth in the group in which trace element solution applied were higher compared to the moment of delivery and the period 3 weeks after birth. In the control group, the fatty acid levels determined 3 weeks before the birth increased at the time of delivery, and the fatty acid levels determined 3 weeks after the birth remained higher than the levels determined 3 weeks before the birth. It was concluded that trace element supplementation applied to pregnant cows during the transition period prevented excessive increase in serum fatty acid levels during the transition period.
{"title":"Effects of trace elements applied to cows in transition period on serum fatty acid profile","authors":"Ö. Kizil, M. Kizil","doi":"10.12681/jhvms.25761","DOIUrl":"https://doi.org/10.12681/jhvms.25761","url":null,"abstract":"The aim of this study was to determine the effects of a trace element solution applied to cows in the transition period on the levels of fatty acids in the transition period. The material of this study consisted of 20 clinically healthy 3-5 year old simental cows kept under the same care and feeding conditions. Pregnant cows used in the study were divided into 2 equal groups with 10 cows in each group. A single dose of 20 ml of a trace element solution was administered intramuscularly to the pregnant cows in the study group 3 weeks before the birth. Blood samples were taken for analysis at the time of trace element administration, the moment of birth and 3 weeks after birth. Serum samples were analyzed using gas chromatography-mass spectrophotometry (GC-MS) device. When the findings of the study were examined, the fatty acid levels determined 3 weeks before the birth in the group in which trace element solution applied were higher compared to the moment of delivery and the period 3 weeks after birth. In the control group, the fatty acid levels determined 3 weeks before the birth increased at the time of delivery, and the fatty acid levels determined 3 weeks after the birth remained higher than the levels determined 3 weeks before the birth. It was concluded that trace element supplementation applied to pregnant cows during the transition period prevented excessive increase in serum fatty acid levels during the transition period.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47444611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Karlidağ, A. Köseman, A. Akyol, G. Saatçıoğlu, I. Şeker, AB Uyumlu, M. Yilmaztekin, S. Erdoğan
In this study, the larvae acceptance rate and the royal jelly yield in honeybee (Apis mellifera L.) colonies supplemented with different industrial sugars at different locations were determined. For this purpose, feeding groups (1. Sucrose group, 2. Glucose group, 3. Bee feed syrup group, 4. Control group) and locations (1. Battalgazi, 2. Doğanşehir) were formed. In queenless colonies that produce royal jelly, in order to sustain 5-15 day-old young feeder worker bees, two sealed frames with brood from support colonies were added. The royal jelly yield was harvested seven times. Based on the location, the feeding groups, and the location x feeding groups interaction, 12600 larvae were grafted, 9054 larvae were accepted, and the larvae acceptance rate was determined as 71.86%. Based on the location, feeding groups and the location x feeding group interaction, the yield per cell was calculated as 213.15 ± 11.53 mg/cell, the yield per colony as 6.88 ± 0.38 g/col./app., and the total yield per colony as 34.40 ± 1.91 g/colony. In the study, no statistically significant difference was determined between feeding with sucrose, bee feed and the supplementary feeding with glucose. On the other hand, it was determined that the location where the royal jelly was produced affected both the larvae acceptance and the royal jelly yield.
{"title":"The effects of different industrial sugars on royal jelly production","authors":"S. Karlidağ, A. Köseman, A. Akyol, G. Saatçıoğlu, I. Şeker, AB Uyumlu, M. Yilmaztekin, S. Erdoğan","doi":"10.12681/jhvms.27794","DOIUrl":"https://doi.org/10.12681/jhvms.27794","url":null,"abstract":"In this study, the larvae acceptance rate and the royal jelly yield in honeybee (Apis mellifera L.) colonies supplemented with different industrial sugars at different locations were determined. For this purpose, feeding groups (1. Sucrose group, 2. Glucose group, 3. Bee feed syrup group, 4. Control group) and locations (1. Battalgazi, 2. Doğanşehir) were formed. In queenless colonies that produce royal jelly, in order to sustain 5-15 day-old young feeder worker bees, two sealed frames with brood from support colonies were added. The royal jelly yield was harvested seven times. Based on the location, the feeding groups, and the location x feeding groups interaction, 12600 larvae were grafted, 9054 larvae were accepted, and the larvae acceptance rate was determined as 71.86%. Based on the location, feeding groups and the location x feeding group interaction, the yield per cell was calculated as 213.15 ± 11.53 mg/cell, the yield per colony as 6.88 ± 0.38 g/col./app., and the total yield per colony as 34.40 ± 1.91 g/colony. In the study, no statistically significant difference was determined between feeding with sucrose, bee feed and the supplementary feeding with glucose. On the other hand, it was determined that the location where the royal jelly was produced affected both the larvae acceptance and the royal jelly yield. ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43342601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in milk goat. Milk samples were precipitated with acetonitrile, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times for tildipirosin and tylosin tartrate were 4.4 min and 10.5 min, respectively. Calibration curves were ranged from 100 to 2500 µg/L. The lower limit of detection was 75 µg/L, and the lower limit of quantitation was 100 µg/L. The accuracy and precision were always <15% except for LOQ < 20%. Mean recovery was 95.6 %. This procedure can be applied to perform pharmacokinetic studies in lactating animals and be useful to detect residues in dairy products.
{"title":"Validation of a LC-UV method for determination of tildipirosin in goat milk","authors":"J. Galecio, V. Hernandis, E. Escudero, P. Marín","doi":"10.12681/jhvms.28300","DOIUrl":"https://doi.org/10.12681/jhvms.28300","url":null,"abstract":"A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in milk goat. Milk samples were precipitated with acetonitrile, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times for tildipirosin and tylosin tartrate were 4.4 min and 10.5 min, respectively. Calibration curves were ranged from 100 to 2500 µg/L. The lower limit of detection was 75 µg/L, and the lower limit of quantitation was 100 µg/L. The accuracy and precision were always <15% except for LOQ < 20%. Mean recovery was 95.6 %. This procedure can be applied to perform pharmacokinetic studies in lactating animals and be useful to detect residues in dairy products.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48420819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Asghar, AA Anjum, M. Rabbani, Mandoji Mansoor Khan, M. Ali, S. Azeem
Thirty puppies were randomly divided in to 3 groups for comparative evaluation of two commercial CPV vaccines. Each group was further subdivided in to < 6 months and < 3 months -old puppies and either vaccinated with a monovalent vaccine: Primodog, a combination vaccine: Duramune or maintained as a non-vaccinated control. Humoral immune response was determined by Hemagglutination Inhibition (HAI) on 21 and 35 -days after vaccination. The geometric mean titer (GMT) induced by Duramune, 21 and 35 -days post-vaccination was GMT 73.3 and 137.2, respectively. Comparatively, Primodog demonstrated higher GMT on 21 and 35 -days after vaccination: 97.0 and 168.9, respectively. The older puppies (< 6 months old) demonstrated higher seroconversion to both vaccines.
{"title":"A commercial monovalent canine parvovirus vaccine performs better than a commercial combination vaccine in puppies","authors":"M. Asghar, AA Anjum, M. Rabbani, Mandoji Mansoor Khan, M. Ali, S. Azeem","doi":"10.12681/jhvms.27960","DOIUrl":"https://doi.org/10.12681/jhvms.27960","url":null,"abstract":"Thirty puppies were randomly divided in to 3 groups for comparative evaluation of two commercial CPV vaccines. Each group was further subdivided in to < 6 months and < 3 months -old puppies and either vaccinated with a monovalent vaccine: Primodog, a combination vaccine: Duramune or maintained as a non-vaccinated control. Humoral immune response was determined by Hemagglutination Inhibition (HAI) on 21 and 35 -days after vaccination. The geometric mean titer (GMT) induced by Duramune, 21 and 35 -days post-vaccination was GMT 73.3 and 137.2, respectively. Comparatively, Primodog demonstrated higher GMT on 21 and 35 -days after vaccination: 97.0 and 168.9, respectively. The older puppies (< 6 months old) demonstrated higher seroconversion to both vaccines.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47339620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Abdel-Rady, I. A. Abd El-Rahim, S. Gad El-Rab Abd El-Hameed, S. Malek
The present study was carried out to record the clinical signs of equine herpesviruses (EHVs) infection and to detect the prevalence of EHVs infection among working equids in different provinces of Egypt. A total number of 115 working equids (92 horses and 23 donkeys) were clinically examined and sampled from November 2018 till November 2019 for this study. Two samples were collected from each animal (nasal swab and blood sample) and were subjected to multiplex-PCR to detect the prevalence of different EHVs infection among equids. In the current study, the overall prevalence of EHVs infection among equid populations in Egypt was 80% by using multiplex-PCR. Moreover, the most prevalent equine herpesvirus (EHV) among equids in Upper Egypt was EHV-2 (61.74%), followed by EHV-5 (43.48%), EHV-1 (20%), and EHV-4 (13.04%). The recorded clinical signs of the examined equids harbored EHVs (PCR-positive) can be summarized as follow: a higher percentage was detected among equids with a history of acute onset (59.78%), pyrexia (57.61%), and/or systemic illness (45.65%) with or without respiratory signs (56.52%) and ocular signs (35.87%). Furthermore, 4.35% and 1.09% of EHV-1 PCR-positive equids displayed neurological signs and abortion, respectively.
{"title":"Clinical and Molecular Epidemiological Study on Herpesviruses Infection among Equid Populations in Upper Egypt","authors":"A. Abdel-Rady, I. A. Abd El-Rahim, S. Gad El-Rab Abd El-Hameed, S. Malek","doi":"10.12681/jhvms.28144","DOIUrl":"https://doi.org/10.12681/jhvms.28144","url":null,"abstract":"The present study was carried out to record the clinical signs of equine herpesviruses (EHVs) infection and to detect the prevalence of EHVs infection among working equids in different provinces of Egypt. A total number of 115 working equids (92 horses and 23 donkeys) were clinically examined and sampled from November 2018 till November 2019 for this study.\u0000Two samples were collected from each animal (nasal swab and blood sample) and were subjected to multiplex-PCR to detect the prevalence of different EHVs infection among equids.\u0000In the current study, the overall prevalence of EHVs infection among equid populations in Egypt was 80% by using multiplex-PCR. Moreover, the most prevalent equine herpesvirus (EHV) among equids in Upper Egypt was EHV-2 (61.74%), followed by EHV-5 (43.48%), EHV-1 (20%), and EHV-4 (13.04%). The recorded clinical signs of the examined equids harbored EHVs (PCR-positive) can be summarized as follow: a higher percentage was detected among equids with a history of acute onset (59.78%), pyrexia (57.61%), and/or systemic illness (45.65%) with or without respiratory signs (56.52%) and ocular signs (35.87%). Furthermore, 4.35% and 1.09% of EHV-1 PCR-positive equids displayed neurological signs and abortion, respectively.\u0000 ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43123139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nephrolithiasis is one of the most important urinary tract diseases of ruminants and a costly problem for many sheep and goat owners. Therefore, it is important to analyse the composition of renal stone to prevent the formation of these kind of stones. The aim of the present study was to determine the frequency and the chemical constituents of urinary stones of Moghani sheep in Northwest of Iran. The kidneys, ureters, and urinary bladders of 384 Moghani sheep were examined carefully. The prevalence of urinary stones was 3.7%. All the stones were in the pelvis of the kidney and no stones were found in the ureter or urinary bladder. Macroscopically, 85.7% of the urinary stones showed a rough (jagged) appearance and 12.3% of them a smooth appearance. The largest and smallest urinary stones were 7 mm and 2 mm in diameter and 0.600 g and 0.087 g in weight, respectively. The composition of calculi were pure or mixed and included calcium oxalate (44.44%), magnesium ammonium phosphate (33.33%) and calcium carbonate (22.22%). Calcium oxalate was the major constituent in the urinary stone, hence, grazing on the pasture plants containing oxalate should be limited. More detail studies should be designed to clarify the contribution of environmental and nutritional factors in development of urinary stones.
{"title":"Study on the frequency and chemical composition of urinary stones of Moghani sheep in Northwest of Iran","authors":"Y. Nozohour, V. Najarnezhad","doi":"10.12681/jhvms.27984","DOIUrl":"https://doi.org/10.12681/jhvms.27984","url":null,"abstract":" Nephrolithiasis is one of the most important urinary tract diseases of ruminants and a costly problem for many sheep and goat owners. Therefore, it is important to analyse the composition of renal stone to prevent the formation of these kind of stones. The aim of the present study was to determine the frequency and the chemical constituents of urinary stones of Moghani sheep in Northwest of Iran. The kidneys, ureters, and urinary bladders of 384 Moghani sheep were examined carefully. The prevalence of urinary stones was 3.7%. All the stones were in the pelvis of the kidney and no stones were found in the ureter or urinary bladder. Macroscopically, 85.7% of the urinary stones showed a rough (jagged) appearance and 12.3% of them a smooth appearance. The largest and smallest urinary stones were 7 mm and 2 mm in diameter and 0.600 g and 0.087 g in weight, respectively. The composition of calculi were pure or mixed and included calcium oxalate (44.44%), magnesium ammonium phosphate (33.33%) and calcium carbonate (22.22%). Calcium oxalate was the major constituent in the urinary stone, hence, grazing on the pasture plants containing oxalate should be limited. More detail studies should be designed to clarify the contribution of environmental and nutritional factors in development of urinary stones.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49351182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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