This study aimed to isolate the Enterococcus species from homemade water buffalo clotted cream sold in Afyonkarahisar, investigate the vancomycin resistance genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. A total of 107 buffalo clotted cream samples sold in public bazaars of Afyonkarahisar were collected. Following the phenotypic identification using a commercial identification kit, PCR was applied to strains using by species specific primers. Forty Enterococcus strains were obtained from 107 samples by PCR. Of 40 strains, 31 (77.5%) and 9 (22.5%) were identified to be E. faecalis and E. faecium, respectively. One vanA (2.5%) and 14 vanB (35%) genes were determined in tested 40 strains. While of strains having the vanB gen, 10 and four were found to be E. faecalis and E. faecium, respectively, one E. faecium strain also harboured the vanA gen. Thus, while the vanB gen positivity was found to be 32.3% in 31 E. faecalis strains and 44.4% in 9 E. faecium strains, the vanA gen positivity was determined to be 11.1% in 9 E. faecium strains. It was not found the vanC1 and vanC2/C3 genes in none of the strains. While all of strains were phenotypically resistant to streptomycin and fusidic acid, high resistance rates were also determined in the strains to kanamycin (87.5%), cephalothin (80%), erythromycin (80%), gentamicin (77.5%), tetracycline (75%) and vancomycin (60%). Resistance to all tested antibiotics except teicoplanin was determined in E. faecalis strains and except chloramphenicol in E. faecium strains harbouring the vancomycin resistance genes. In conclusion, it was thought the buffalo clotted creams offered for consumption in Afyonkarahisar may have potential risk for public health in terms of VRE species. To our knowledge, this is the first study showing the presence of VRE in the clotted cream samples by PCR in Turkey.
{"title":"First report of vancomycin resistant Enterococcus faecalis and Enterococcus faecium isolated from water buffalo clotted cream in Turkey","authors":"E Seker, YN Dogan","doi":"10.12681/jhvms.30877","DOIUrl":"https://doi.org/10.12681/jhvms.30877","url":null,"abstract":"This study aimed to isolate the Enterococcus species from homemade water buffalo clotted cream sold in Afyonkarahisar, investigate the vancomycin resistance genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. A total of 107 buffalo clotted cream samples sold in public bazaars of Afyonkarahisar were collected. Following the phenotypic identification using a commercial identification kit, PCR was applied to strains using by species specific primers. Forty Enterococcus strains were obtained from 107 samples by PCR. Of 40 strains, 31 (77.5%) and 9 (22.5%) were identified to be E. faecalis and E. faecium, respectively. One vanA (2.5%) and 14 vanB (35%) genes were determined in tested 40 strains. While of strains having the vanB gen, 10 and four were found to be E. faecalis and E. faecium, respectively, one E. faecium strain also harboured the vanA gen. Thus, while the vanB gen positivity was found to be 32.3% in 31 E. faecalis strains and 44.4% in 9 E. faecium strains, the vanA gen positivity was determined to be 11.1% in 9 E. faecium strains. It was not found the vanC1 and vanC2/C3 genes in none of the strains. While all of strains were phenotypically resistant to streptomycin and fusidic acid, high resistance rates were also determined in the strains to kanamycin (87.5%), cephalothin (80%), erythromycin (80%), gentamicin (77.5%), tetracycline (75%) and vancomycin (60%). Resistance to all tested antibiotics except teicoplanin was determined in E. faecalis strains and except chloramphenicol in E. faecium strains harbouring the vancomycin resistance genes. In conclusion, it was thought the buffalo clotted creams offered for consumption in Afyonkarahisar may have potential risk for public health in terms of VRE species. To our knowledge, this is the first study showing the presence of VRE in the clotted cream samples by PCR in Turkey.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the early diagnosability of superficial and visceral caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep isolated from C. pseudotuberculosis were compared with healthy sheep, it was determined that there was a statistically significant decrease in the levels of antioxidant molecules such as GSH-pX (14.62%), GSH (23.81%), SOD (4.70%), CAT (22.23%) (p˂0.001). The level of toxic malondialdehyde (MDA) (18.62%), the end product of lipid peroxidation, was found to be statistically significantly increased in sheep isolated from C. pseudotuberculosis (p˂0.001). As a result, it was determined that oxidative stress factors showed statistically significant variability in cases of superficial caseous lymphadenitis (caused by C.pseudotuberculosis). For this reason, by determining the levels of oxidative stress factors in suspected herds, it was possible to make early diagnosis of the superficial and visceral forms of caseous lymphadenitis, and a basis was established for future studies.
{"title":"Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors","authors":"E Polat, E Kaya, B Karagülle, H Akin","doi":"10.12681/jhvms.31218","DOIUrl":"https://doi.org/10.12681/jhvms.31218","url":null,"abstract":"In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the early diagnosability of superficial and visceral caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep isolated from C. pseudotuberculosis were compared with healthy sheep, it was determined that there was a statistically significant decrease in the levels of antioxidant molecules such as GSH-pX (14.62%), GSH (23.81%), SOD (4.70%), CAT (22.23%) (p˂0.001). The level of toxic malondialdehyde (MDA) (18.62%), the end product of lipid peroxidation, was found to be statistically significantly increased in sheep isolated from C. pseudotuberculosis (p˂0.001). As a result, it was determined that oxidative stress factors showed statistically significant variability in cases of superficial caseous lymphadenitis (caused by C.pseudotuberculosis). For this reason, by determining the levels of oxidative stress factors in suspected herds, it was possible to make early diagnosis of the superficial and visceral forms of caseous lymphadenitis, and a basis was established for future studies.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F Talazadeh, MH Razijalali, N Roshanzadeh, P Davoodi
Considering the increase in the keeping of pet birds and the importance of gastrointestinal parasitic infection, especially zoonotic parasites, this study was conducted to determine the prevalence of gastrointestinal parasites in different species of Passeriformes and Psittaciformes. In this study, one hundred and twenty fresh fecal samples were collected from different species of captive pet birds in Ahvaz from Apr 2021 to Aug 2021. The collected samples were analyzed using Clayton-Lane methods, Modified Ziehl–Neelsen, and Trichrome staining. Light microscopic morphometry was used for the identification of helminth eggs and oocysts. The results of this study showed that 37 samples (30.83%) were infected with gastrointestinal parasites. Among the helminthic parasites, three samples (2.5%) were infected with Hymenolepis spp.. Among protozoan parasites, 24 samples (20%) were infected with Eimeria spp., three samples (2.5%) were infected with Isospora spp., two samples (1.66%) were infected with Cryptosporidium spp., and five samples (4.16%) were infected with Giardia spp.. The results of the present study showed that the highest rate of gastrointestinal parasitic infection was in Passeriformes kept in pet shops and the prevalence of gastrointestinal parasitic infection was higher in the zebra finch, common mynah, and canary compared to other pet birds. But the highest rate of zoonotic parasitic infection was in Psittaciformes. According to the results, zoonotic parasites are present in pet birds in the Ahvaz area and should be considered by the owners of these pet birds, breeders, veterinarians, and public health organizations.
{"title":"Survey on the gastrointestinal parasites in Passeriformes and Psittaciformes with a focus on zoonotic parasites","authors":"F Talazadeh, MH Razijalali, N Roshanzadeh, P Davoodi","doi":"10.12681/jhvms.31398","DOIUrl":"https://doi.org/10.12681/jhvms.31398","url":null,"abstract":"Considering the increase in the keeping of pet birds and the importance of gastrointestinal parasitic infection, especially zoonotic parasites, this study was conducted to determine the prevalence of gastrointestinal parasites in different species of Passeriformes and Psittaciformes. In this study, one hundred and twenty fresh fecal samples were collected from different species of captive pet birds in Ahvaz from Apr 2021 to Aug 2021. The collected samples were analyzed using Clayton-Lane methods, Modified Ziehl–Neelsen, and Trichrome staining. Light microscopic morphometry was used for the identification of helminth eggs and oocysts. The results of this study showed that 37 samples (30.83%) were infected with gastrointestinal parasites. Among the helminthic parasites, three samples (2.5%) were infected with Hymenolepis spp.. Among protozoan parasites, 24 samples (20%) were infected with Eimeria spp., three samples (2.5%) were infected with Isospora spp., two samples (1.66%) were infected with Cryptosporidium spp., and five samples (4.16%) were infected with Giardia spp.. The results of the present study showed that the highest rate of gastrointestinal parasitic infection was in Passeriformes kept in pet shops and the prevalence of gastrointestinal parasitic infection was higher in the zebra finch, common mynah, and canary compared to other pet birds. But the highest rate of zoonotic parasitic infection was in Psittaciformes. According to the results, zoonotic parasites are present in pet birds in the Ahvaz area and should be considered by the owners of these pet birds, breeders, veterinarians, and public health organizations.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The nutritive value and invitro digestibility of agro-industrial byproducts of Citrus aurantium, Citrus paradisi, Citrus reticulata, Citrus limon and Citrus sinensis were assessed for their feed modification potential. Nutritive value assessment was done through chemical analysis while the ANKOM Daisy II incubator method was used to determine the invitro digestibility. At p>0.05, there was no statistical significance for nutritive value of the citrus byproducts for the five species. The agro-industrial by-products had significantly different phenolic content. Invitro digestibility of the peels significantly differed from the pomaces (p<0.001). The byproducts of the five citrus species showed potential to be considered as non-forage energy sources in ruminant feed. Lemon byproducts had high protein but not sufficient as a single protein source while bitter orange pomace may be considered for antioxidant capacity and high digestibility. Abundance of citrus agro-industrial byproducts, nutritive value potentials and their digestibility makes it a good strategy for ruminant feed modification and utilization.
{"title":"Nutritive value and in-vitro digestibility of peels and pomaces of different citrus species","authors":"OO Olowu, S Yaman Firincioglu","doi":"10.12681/jhvms.31044","DOIUrl":"https://doi.org/10.12681/jhvms.31044","url":null,"abstract":"The nutritive value and invitro digestibility of agro-industrial byproducts of Citrus aurantium, Citrus paradisi, Citrus reticulata, Citrus limon and Citrus sinensis were assessed for their feed modification potential. Nutritive value assessment was done through chemical analysis while the ANKOM Daisy II incubator method was used to determine the invitro digestibility. At p>0.05, there was no statistical significance for nutritive value of the citrus byproducts for the five species. The agro-industrial by-products had significantly different phenolic content. Invitro digestibility of the peels significantly differed from the pomaces (p<0.001). The byproducts of the five citrus species showed potential to be considered as non-forage energy sources in ruminant feed. Lemon byproducts had high protein but not sufficient as a single protein source while bitter orange pomace may be considered for antioxidant capacity and high digestibility. Abundance of citrus agro-industrial byproducts, nutritive value potentials and their digestibility makes it a good strategy for ruminant feed modification and utilization.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The goal of this study was to detect the influence of royal jelly which has a strong antioxidant capacity supplementation in TRIS egg yolk and TRIS soybean lecithin extenders on quality and fertility. Sperm was collected from 5 Damascus bucks via an electro ejaculator during the breeding season. After dilution and cooling, the lowest motility and membrane integrity were detected in TEY-1RJ, in terms of abnormal spermatozoon rate, the TEY-1RJ group was the highest in cooled semen (P<0.05). After dilution and cooling, osmolality decreased significantly when the RJ ratio increased (P<0.05). After freezing-thawing, in both TEY and TSL extenders, the lowest sperm quality was detected in the 1% RJ groups. In terms of intact acrosome ratio, the highest value was recorded in the 0.50% RJ group in TEY and TSL diluents (P<0.05). Fertility assessments were conducted with 150 goats synchronized with intravaginal sponges and goats were inseminated transcervical. There was no difference between pregnancy rates between TEY and TSL-control and RJ groups (P>0.05). As a result, it was deduced that RJ additions to TEY and TSL extenders did not produce any alternative to freezing on sperm quality in Damascus goat semen except for the intact acrosome ratio and had no positive effect on fertility.
{"title":"The influence of various rates of royal jelly enriched extenders on frozen/thawed sperm quality and fertility in goats","authors":"N Coskun Cetin, F Karaca","doi":"10.12681/jhvms.30798","DOIUrl":"https://doi.org/10.12681/jhvms.30798","url":null,"abstract":"The goal of this study was to detect the influence of royal jelly which has a strong antioxidant capacity supplementation in TRIS egg yolk and TRIS soybean lecithin extenders on quality and fertility. Sperm was collected from 5 Damascus bucks via an electro ejaculator during the breeding season. After dilution and cooling, the lowest motility and membrane integrity were detected in TEY-1RJ, in terms of abnormal spermatozoon rate, the TEY-1RJ group was the highest in cooled semen (P<0.05). After dilution and cooling, osmolality decreased significantly when the RJ ratio increased (P<0.05). After freezing-thawing, in both TEY and TSL extenders, the lowest sperm quality was detected in the 1% RJ groups. In terms of intact acrosome ratio, the highest value was recorded in the 0.50% RJ group in TEY and TSL diluents (P<0.05). Fertility assessments were conducted with 150 goats synchronized with intravaginal sponges and goats were inseminated transcervical. There was no difference between pregnancy rates between TEY and TSL-control and RJ groups (P>0.05). As a result, it was deduced that RJ additions to TEY and TSL extenders did not produce any alternative to freezing on sperm quality in Damascus goat semen except for the intact acrosome ratio and had no positive effect on fertility.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of the present study was to identify the effects of in-ovo injection of glucose and glutamine on hatching efficiency, hatching weight, liver and residual yolk weights, intestinal histomorphology and gene expressions of digestive enzymes. On the 17th day of incubation, NaCl (0.9 %), glutamine (0.5 ml 10%), glucose (0.5 ml 0.25 g/ml), and glutamine+glucose (0.25 ml 10%+0.25 ml 0.25 g/ml) were injected into the amniotic sac. In-ovo injection of glucose, glutamine and glucose+glutamine significantly decreased hatching efficiency in glucose and glutamin+glucose groups (p<0.001). This treatment did not affect hatching weight, liver and residual yolk weight in any of the groups. When the intestinal histomorphology was evaluated, in-ovo injection was found to increase the villi height/crypt depth ratio, villus width and the number of goblet cells in the jejunum; whereas villi height, crypt depth, and tunica muscularis thickness were not significantly affected by the treatments. Moreover, the ileum histomorphology in general appear not to be affected by any of the treatments as well. In-ovo glutamine injection significantly increased Suc-Iso and mTOR gene expressions (p≤0.05) compared to the control group, whereas SGLT1 gene expression was statistically indifferent. At the end of the study, while in-ovo glucose and glutamine injection had an overall negative effect on the hatching efficiency in broiler chicks.
本试验旨在研究卵内注射葡萄糖和谷氨酰胺对雏鸡孵化效率、孵化重、肝脏和残余蛋黄重、肠道组织形态和消化酶基因表达的影响。培养第17天，羊膜囊内注射NaCl(0.9%)、谷氨酰胺(0.5 ml 10%)、葡萄糖(0.5 ml 0.25 g/ml)、谷氨酰胺+葡萄糖(0.25 ml 10%+0.25 ml 0.25 g/ml)。卵内注射葡萄糖、谷氨酰胺和葡萄糖+谷氨酰胺显著降低了葡萄糖组和谷氨酰胺+葡萄糖组的孵化效率(p<0.001)。该处理对各组的孵化重、肝脏和残余蛋黄重均无影响。观察肠组织形态学，发现卵内注射增加了空肠绒毛高度/隐窝深度比、绒毛宽度和杯状细胞数量;而绒毛高度、隐窝深度和肌膜厚度则不受处理的显著影响。此外，回肠的组织形态总体上也不会受到任何治疗的影响。蛋清谷氨酰胺注射液显著提高了su - iso和mTOR基因表达量(p≤0.05)，而SGLT1基因表达量与对照组相比无统计学差异。研究结束时，蛋内葡萄糖和谷氨酰胺注射液对肉仔鸡的孵化效率总体呈负影响。
{"title":"Effects of In-Ovo Glucose and Glutamine Treatment on Hatching Efficiency, Intestinal Histomorphology and Gene Expression of Digestive Enzymes in Broiler Chicks","authors":"F Cönerr, P Saçaklı","doi":"10.12681/jhvms.30895","DOIUrl":"https://doi.org/10.12681/jhvms.30895","url":null,"abstract":"The purpose of the present study was to identify the effects of in-ovo injection of glucose and glutamine on hatching efficiency, hatching weight, liver and residual yolk weights, intestinal histomorphology and gene expressions of digestive enzymes. On the 17th day of incubation, NaCl (0.9 %), glutamine (0.5 ml 10%), glucose (0.5 ml 0.25 g/ml), and glutamine+glucose (0.25 ml 10%+0.25 ml 0.25 g/ml) were injected into the amniotic sac. In-ovo injection of glucose, glutamine and glucose+glutamine significantly decreased hatching efficiency in glucose and glutamin+glucose groups (p<0.001). This treatment did not affect hatching weight, liver and residual yolk weight in any of the groups. When the intestinal histomorphology was evaluated, in-ovo injection was found to increase the villi height/crypt depth ratio, villus width and the number of goblet cells in the jejunum; whereas villi height, crypt depth, and tunica muscularis thickness were not significantly affected by the treatments. Moreover, the ileum histomorphology in general appear not to be affected by any of the treatments as well. In-ovo glutamine injection significantly increased Suc-Iso and mTOR gene expressions (p≤0.05) compared to the control group, whereas SGLT1 gene expression was statistically indifferent. At the end of the study, while in-ovo glucose and glutamine injection had an overall negative effect on the hatching efficiency in broiler chicks.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study is to improve the nutritional value of sunflower meal by solid state fermentation (SSF) using agricultural wastes such as whey and pomegranate peel. Experiment was conducted with six groups. First group SM (sunflower meal) + TW (tap water), the second group SM + W (whey), the third group SM + W + 0.5% PP, the fourth group SM + W + 1% PP, the fifth group SM + W + 1.5% PP and the sixth group SM + W + 2% PP, respectively. The mixtures were prepared to complete 100 grams of sunflower meal with 0, 0,5, 1,0, 1,5 and 2,0 grams of pomegranate peel (PP), respectively. Each group consisted of eight replicates. Prepared mixtures were placed in 500 ml erlenmayers and 120 ml tap water was added to the mixing of first group and 120 ml whey was added in other groups and then mixed homogeneously. Four of the erlenmayers prepared for each group without being fermented were dried at room temperature. The remaining erlenmayers were fermented in 32°C±2 for 48 hours. Dry matter, crude protein, ether extract, crude ash, crude fiber, phytic acid ratios and phytase activities, antioxidant activities and yeast (Saccharomyces cerevisiae) numbers were determined in fermented and non-fermented feed samples. As a result of solid state fermentation with whey by adding pomegranate peel to sunflower meal, crude protein ratio, antioxidant activity and yeast count increased, and phytic acid ratio decreased. In conclusion, sunflower meal with functional properties was obtained. In addition, wastes such as whey and pomegranate peel were used as feed and environmental pollution was prevented. Keywords: Sunflower meal; pomegranate peel; whey; solid state fermentation; functional meal
本研究旨在利用农业废弃物乳清、石榴皮等进行固态发酵，提高葵花籽粕的营养价值。试验共设6组。第一组SM(葵花籽粕)+ TW(自来水)、第二组SM + W(乳清)、第三组SM + W + 0.5% PP、第四组SM + W + 1% PP、第五组SM + W + 1.5% PP和第六组SM + W + 2% PP。分别用0、0、5、1、0、1、5、5、2克石榴皮(PP)完成100克葵花籽粕。每组8个重复。将配制好的混合液置于500 ml瓶中，第一组加入120 ml自来水，其他组加入120 ml乳清，混合均匀。每组各取4个未发酵的二奶，在室温下干燥。剩余的酵母在32℃±2条件下发酵48小时。测定发酵和非发酵饲料样品的干物质、粗蛋白质、粗脂肪、粗灰分、粗纤维、植酸比、植酸酶活性、抗氧化活性和酵母(酿酒酵母)数量。
在向日葵粕中添加石榴皮与乳清进行固体发酵，提高了粗蛋白质比、抗氧化活性和酵母数，降低了植酸比。综上所述，获得了具有功能特性的葵花籽粕。此外，将乳清、石榴皮等废弃物用作饲料，防止了环境污染。
关键词:葵花籽粕;石榴皮;乳清蛋白;固态发酵;功能性餐
{"title":"Effect of Solid State Fermentation with Whey on Nutrient Composition of Pomegranate Peel Supplemented Sunflower Meal","authors":"B Dumlu, ŞC Bölükbaşı","doi":"10.12681/jhvms.31023","DOIUrl":"https://doi.org/10.12681/jhvms.31023","url":null,"abstract":"The aim of the study is to improve the nutritional value of sunflower meal by solid state fermentation (SSF) using agricultural wastes such as whey and pomegranate peel. \u0000Experiment was conducted with six groups. First group SM (sunflower meal) + TW (tap water), the second group SM + W (whey), the third group SM + W + 0.5% PP, the fourth group SM + W + 1% PP, the fifth group SM + W + 1.5% PP and the sixth group SM + W + 2% PP, respectively. The mixtures were prepared to complete 100 grams of sunflower meal with 0, 0,5, 1,0, 1,5 and 2,0 grams of pomegranate peel (PP), respectively. Each group consisted of eight replicates. Prepared mixtures were placed in 500 ml erlenmayers and 120 ml tap water was added to the mixing of first group and 120 ml whey was added in other groups and then mixed homogeneously. Four of the erlenmayers prepared for each group without being fermented were dried at room temperature. The remaining erlenmayers were fermented in 32°C±2 for 48 hours. Dry matter, crude protein, ether extract, crude ash, crude fiber, phytic acid ratios and phytase activities, antioxidant activities and yeast (Saccharomyces cerevisiae) numbers were determined in fermented and non-fermented feed samples. \u0000As a result of solid state fermentation with whey by adding pomegranate peel to sunflower meal, crude protein ratio, antioxidant activity and yeast count increased, and phytic acid ratio decreased. In conclusion, sunflower meal with functional properties was obtained. In addition, wastes such as whey and pomegranate peel were used as feed and environmental pollution was prevented. \u0000Keywords: Sunflower meal; pomegranate peel; whey; solid state fermentation; functional meal","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Adwan, S Abuseir, O Khreishi, A Hussein, N Khraim, M Abed Al-Daym
Salmonella is one of the most frequently isolated foodborne pathogens. It is of major public health concern worldwide. Poultry meat and eggs represent an important source of Salmonellae organism for consumer health. This study aimed to evaluate the occurrence of Salmonella enterica serotype Typhimurium and Enteritidis using multiplex PCR (mPCR) among isolates collected from the local market and to assess genetic relationships between isolates of S. Typhimurium, which was the only serotype isolated from the tested food samples. This was done using virulence factors profiling and fingerprint profiling by random amplified polymorphic DNA (RAPD-PCR) and repetitive sequence PCR (REP-PCR) using enterobacterial repetitive intergenic consensus (ERIC-PCR) and interspersed repetitive DNA sequence BOXAIR-PCR.
The overall occurrence percentage of S. Typhimurium and S. Enteritidis out of 51 isolates was 54.9% and 0.0%, respectively. Only 13 out of 17 virulence genes were detected in these isolates. The occurrence of the detected virulence genes among these isolates was 100%, 50.0%,46.4%, 39.3%, 35.7%, 35.7%, 32.1%, 25.0%, 25.0%, 17.6%, 14.3%, 14.3%, 3.6% for invA, sopB, prgH, sitC, pefA, tolC, cdtB, msgA, sifA, iroN, spiA, ipfC and pagC, respectively. The remaining virulence genes were absent in all of the isolates. Based on the combination of the presence and absence of virulence genes, eight profiles were detected among these isolates, the most common genetic profile was V5 (each 32.1%). Based on this genetic profile at cut-off point 96.0%, both ERIC and BOX primers allowed for discrimination into 4 and 6 clusters or clones of 16 S. Typhimurium isolates, respectively. Results of PCR typing methods showed that, three strains clustered together using both ERIC-PCR and BOX-PCR typing methods and they had the same virulotype (V1), while other four strains also clustered together by both typing methods and had the same virulotype (V8).
Contamination of food with Salmonellae especially with S. Typhimurium was high and indicated a bad microbiological quality of food. This emphasizes the need for rigorous public health and food safety methods to lower the human health hazard and risk associated with Salmonellae infection.
{"title":"Occurrence and molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis isolates from contaminated food samples from Palestine","authors":"G Adwan, S Abuseir, O Khreishi, A Hussein, N Khraim, M Abed Al-Daym","doi":"10.12681/jhvms.31088","DOIUrl":"https://doi.org/10.12681/jhvms.31088","url":null,"abstract":"Salmonella is one of the most frequently isolated foodborne pathogens. It is of major public health concern worldwide. Poultry meat and eggs represent an important source of Salmonellae organism for consumer health. This study aimed to evaluate the occurrence of Salmonella enterica serotype Typhimurium and Enteritidis using multiplex PCR (mPCR) among isolates collected from the local market and to assess genetic relationships between isolates of S. Typhimurium, which was the only serotype isolated from the tested food samples. This was done using virulence factors profiling and fingerprint profiling by random amplified polymorphic DNA (RAPD-PCR) and repetitive sequence PCR (REP-PCR) using enterobacterial repetitive intergenic consensus (ERIC-PCR) and interspersed repetitive DNA sequence BOXAIR-PCR.
 The overall occurrence percentage of S. Typhimurium and S. Enteritidis out of 51 isolates was 54.9% and 0.0%, respectively. Only 13 out of 17 virulence genes were detected in these isolates. The occurrence of the detected virulence genes among these isolates was 100%, 50.0%,46.4%, 39.3%, 35.7%, 35.7%, 32.1%, 25.0%, 25.0%, 17.6%, 14.3%, 14.3%, 3.6% for invA, sopB, prgH, sitC, pefA, tolC, cdtB, msgA, sifA, iroN, spiA, ipfC and pagC, respectively. The remaining virulence genes were absent in all of the isolates. Based on the combination of the presence and absence of virulence genes, eight profiles were detected among these isolates, the most common genetic profile was V5 (each 32.1%). Based on this genetic profile at cut-off point 96.0%, both ERIC and BOX primers allowed for discrimination into 4 and 6 clusters or clones of 16 S. Typhimurium isolates, respectively. Results of PCR typing methods showed that, three strains clustered together using both ERIC-PCR and BOX-PCR typing methods and they had the same virulotype (V1), while other four strains also clustered together by both typing methods and had the same virulotype (V8).
 Contamination of food with Salmonellae especially with S. Typhimurium was high and indicated a bad microbiological quality of food. This emphasizes the need for rigorous public health and food safety methods to lower the human health hazard and risk associated with Salmonellae infection.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B Tras, K Uney, H Eser Faki, T Melik Parlak, Z Ozdemir Kutahya
The study was investigated the effect of different doses of levothyroxine on the mRNA expression, protein level and function of Pgp. Mice were divided into 6 groups as control, low dose levothyroxine, high dose levothyroxine, fexofenadine, low dose levothyroxine+fexofenadine and high dose levothyroxine+fexofenadine. Mice received levothyroxine at doses of 8 and 80 µg/kg daily for 21 days. Fexofenadine was administered at dose of 40 mg/kg at the 24 h following the last administration of levothyroxine. The mRNA levels and protein level of Pgp in liver and small intestine were determined by RT-PCR and western blot analysis, respectively. Plasma concentrations of fexofenadine were determined using HPLC. Levothyroxine at low and high doses caused an insignificant increase intestinal mRNA expression of mdr1a, while high dose levothyroxine+fexofenadine caused a significant increase. Levothyroxine caused a dose-dependent decrease in intestinal mRNA expression of mdr1b. In liver, levothyroxine caused a dose-dependent increase in the mRNA expression of mdr1a. Fexofenadine significantly reduced the effect of levothyroxine on mRNA expression of mdr1a in liver. Levothyroxine increased the protein level of Pgp in liver and decrease in intestines. Low dose levothyroxine significantly increased the plasma concentration of fexofenadine. The effects of levothyroxine on the mRNA expression of mdr1a and b in small intestine and liver and protein level of Pgp varied depending on the dose, tissue type, and fexofenadine administration.
{"title":"The effect of low- and high-dose levothyroxine on the expression, protein level, and function of P-glycoprotein in mice","authors":"B Tras, K Uney, H Eser Faki, T Melik Parlak, Z Ozdemir Kutahya","doi":"10.12681/jhvms.31421","DOIUrl":"https://doi.org/10.12681/jhvms.31421","url":null,"abstract":"The study was investigated the effect of different doses of levothyroxine on the mRNA expression, protein level and function of Pgp. Mice were divided into 6 groups as control, low dose levothyroxine, high dose levothyroxine, fexofenadine, low dose levothyroxine+fexofenadine and high dose levothyroxine+fexofenadine. Mice received levothyroxine at doses of 8 and 80 µg/kg daily for 21 days. Fexofenadine was administered at dose of 40 mg/kg at the 24 h following the last administration of levothyroxine. The mRNA levels and protein level of Pgp in liver and small intestine were determined by RT-PCR and western blot analysis, respectively. Plasma concentrations of fexofenadine were determined using HPLC. Levothyroxine at low and high doses caused an insignificant increase intestinal mRNA expression of mdr1a, while high dose levothyroxine+fexofenadine caused a significant increase. Levothyroxine caused a dose-dependent decrease in intestinal mRNA expression of mdr1b. In liver, levothyroxine caused a dose-dependent increase in the mRNA expression of mdr1a. Fexofenadine significantly reduced the effect of levothyroxine on mRNA expression of mdr1a in liver. Levothyroxine increased the protein level of Pgp in liver and decrease in intestines. Low dose levothyroxine significantly increased the plasma concentration of fexofenadine. The effects of levothyroxine on the mRNA expression of mdr1a and b in small intestine and liver and protein level of Pgp varied depending on the dose, tissue type, and fexofenadine administration.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to determine the effects of intramuscular administration of butorphanol with and without medetomidine on intraocular pressure (IOP) in cats. Sixteen clinically normal cats were used. Cats were randomly received two-treatment regimen. The first regimen was 0.2mg/kg butorphanol, and the second regimen was a mixture of 0.1mg/kg butorphanol and 0.05mg/kg medetomidine. IOP values were recorded before treatment (T0) and following the treatment at 10 (T10), 20 (T20), 30 (T30), and 40 min (T40) in both groups. Administration of butorphanol with and without medetomidine did not cause a statistically significant change in IOP values. The IOP did not change over time (p = 0.41). The mean values of IOP in butorphanol, and butorphanol-medetomidine groups were 20.00 ± 2.29 and 20.38 ± 2.35 mm Hg, respectively. In conclusion, intramuscular administration of butorphanol with or without medetomidine had no significant effect on IOP in cats.
本研究旨在确定肌注布托啡诺加美托咪定和不加美托咪定对猫眼压(IOP)的影响。使用16只临床正常的猫。猫随机接受两种治疗方案。第一方案为0.2mg/kg丁托酚，第二方案为0.1mg/kg丁托酚与0.05mg/kg美托咪定混合。分别记录两组患者治疗前(T0)和治疗后10 (T10)、20 (T20)、30 (T30)、40 min (T40)的IOP值。布托啡诺联合或不联合美托咪定均未引起IOP值的统计学显著变化。IOP没有随时间变化(p = 0.41)。布托啡诺组和布托啡诺-美托咪定组眼压平均值分别为20.00±2.29和20.38±2.35 mm Hg。综上所述，肌注布托啡诺加或不加美托咪定对猫眼压无显著影响。
{"title":"Effect of Butorphanol with and without Medetomidine on Intraocular Pressure in Cats","authors":"N Mohammadi, LE Yanmaz","doi":"10.12681/jhvms.26572","DOIUrl":"https://doi.org/10.12681/jhvms.26572","url":null,"abstract":"This study aimed to determine the effects of intramuscular administration of butorphanol with and without medetomidine on intraocular pressure (IOP) in cats. Sixteen clinically normal cats were used. Cats were randomly received two-treatment regimen. The first regimen was 0.2mg/kg butorphanol, and the second regimen was a mixture of 0.1mg/kg butorphanol and 0.05mg/kg medetomidine. IOP values were recorded before treatment (T0) and following the treatment at 10 (T10), 20 (T20), 30 (T30), and 40 min (T40) in both groups. Administration of butorphanol with and without medetomidine did not cause a statistically significant change in IOP values. The IOP did not change over time (p = 0.41). The mean values of IOP in butorphanol, and butorphanol-medetomidine groups were 20.00 ± 2.29 and 20.38 ± 2.35 mm Hg, respectively. In conclusion, intramuscular administration of butorphanol with or without medetomidine had no significant effect on IOP in cats.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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