A 10-year-old intact male Kangal dog presented with a mass located in the left stifle joint. The mass was 1.2x0.8x1 cm in size. Hematoxylin-eosin and immunohistochemical staining methods were used for histopathological evaluation. Histopathological examination revealed a significant proliferation in synoviocytes. Also, vascular channels lined with well-differentiated endothelial cells were detected. Immunohistochemically, mild CD31 expression was observed in the endothelial cells of the vascular channels. Actin expression was detected in smooth muscle cells of the vascular channels. In addition, vimentin expression was observed in the stromal connective tissue around blood vessels. Based on these findings, the mass was diagnosed as synovial hemangioma. In this case report, synovial hemangioma is rarely observed in animals has been reported.
{"title":"Synovial Hemangioma of the Stifle Joint in a Kangal Dog","authors":"B. Dayı, A. Sevimli","doi":"10.12681/jhvms.30217","DOIUrl":"https://doi.org/10.12681/jhvms.30217","url":null,"abstract":"A 10-year-old intact male Kangal dog presented with a mass located in the left stifle joint. The mass was 1.2x0.8x1 cm in size. Hematoxylin-eosin and immunohistochemical staining methods were used for histopathological evaluation. Histopathological examination revealed a significant proliferation in synoviocytes. Also, vascular channels lined with well-differentiated endothelial cells were detected. Immunohistochemically, mild CD31 expression was observed in the endothelial cells of the vascular channels. Actin expression was detected in smooth muscle cells of the vascular channels. In addition, vimentin expression was observed in the stromal connective tissue around blood vessels. Based on these findings, the mass was diagnosed as synovial hemangioma. In this case report, synovial hemangioma is rarely observed in animals has been reported.\u0000 ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SS Birincioğlu, H. Avcı, E. Ipek, E. Berke, B. Birincioğlu, A. Aydogan
Sphaerospora dicentrarchi is typically histozoic parasite of the wild and cultured European sea bass (Dicentrachus labrax) of the Mediterranean coastal countries. In this study, pathological and ultrastructural findings of the Sphaerospora dicentrarchi infection in European sea bass were firstly investigated in Turkey. For this purpose, the 862 European seabass (Dicentrarchus labrax L) were collected and evaluated from eight cultivation fish farms (offshore floating cages) in four different locations of Aegean Sea of Turkey (three farms from Kazıklı, one farm from Çandarlı, two farms from Taşburun and two farms from Güllük Regions). It was observed that the parasite was especially intense in adult fish and in the June-October period when the water temperature increased. Microscopically, the parasites were seen as the most intensive in the stomach, intestines, gall bladder, pancreas, heart and testis, respectively. Stomach was the most commonly infected organ. In the transmission electron microscopy examination, a bag-like group of mature couple spores were seen surrounded by polar capsules. As a result, it was determined that Sphaerospora dicentrarchi infection is very common in adult sea bass in the Aegean region of Turkey, as in Greece, Spain, Portugal and Italy.
{"title":"Pathological and Ultrastructural Findings of Sphaerospora dicentrarchi (Myxosporea: Bivalvulida) infection in European seabass (Dicentrarchus labrax) farmed in Turkey","authors":"SS Birincioğlu, H. Avcı, E. Ipek, E. Berke, B. Birincioğlu, A. Aydogan","doi":"10.12681/jhvms.29339","DOIUrl":"https://doi.org/10.12681/jhvms.29339","url":null,"abstract":"Sphaerospora dicentrarchi is typically histozoic parasite of the wild and cultured European sea bass (Dicentrachus labrax) of the Mediterranean coastal countries. In this study, pathological and ultrastructural findings of the Sphaerospora dicentrarchi infection in European sea bass were firstly investigated in Turkey. For this purpose, the 862 European seabass (Dicentrarchus labrax L) were collected and evaluated from eight cultivation fish farms (offshore floating cages) in four different locations of Aegean Sea of Turkey (three farms from Kazıklı, one farm from Çandarlı, two farms from Taşburun and two farms from Güllük Regions). It was observed that the parasite was especially intense in adult fish and in the June-October period when the water temperature increased. Microscopically, the parasites were seen as the most intensive in the stomach, intestines, gall bladder, pancreas, heart and testis, respectively. Stomach was the most commonly infected organ. In the transmission electron microscopy examination, a bag-like group of mature couple spores were seen surrounded by polar capsules. As a result, it was determined that Sphaerospora dicentrarchi infection is very common in adult sea bass in the Aegean region of Turkey, as in Greece, Spain, Portugal and Italy.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışmada, belirli oranlarda çiçek ve çam balının koç spermatının spermatolojik parametreleri üzerine etkileri araştırılmıştır. Tris-yumurta sarısı bazlı meni sulandırıcıya her bal türü için %1.0 (h/h) ve %2.5 (h/h) oranlarında çiçek ve çam balı eklenmiştir. Sperm toplama için beş Kıvircik koç kullanıldı. Toplanan spermler havuzlamanın ardından 5 eşit parçaya bölündü. %1.0 (h/h) ve %2.5 (h/v) çam balı ve %1.0 (h/v) ve %2.5 (h/h) çiçek balı oranlarında ve bal ilavesi yapılmadan iki aşamalı seyreltme yöntemi kullanılmıştır. Tris-yumurta sarısı bazlı meni genişleticiye. Numuneler daha sonra sıvı nitrojen buharında dondu ve sıvı nitrojen içinde saklandı. Semen canlılığı parametreleri (toplam ve progresif motilite, hız, kinetik hareket parametreleri ve mitokondriyal aktivite) ve morfolojik bütünlük parametreleri (Hancock fiksasyon testi, akış sitometrisi ve floresan boyama) dengeleme ve çözdürmeden sonra değerlendirildi. Çalışma boyunca on tekrarlı çalışma yapılmıştır.Sonuçlar çiçek balının çözülme sonrası canlılık parametreleri üzerinde özellikle %2,5 (h/h) oranında faydalı etkiye sahip olduğunu göstermiştir. Her oranda ve her çeşit bal içeren sulandırıcılar, çözüldükten sonra zar bütünlüğünü korumuştur. Tris-yumurta sarısı bazlı sulandırıcıya %1.0 (v/v) çiçek balı ilavesi, diğer gruplara kıyasla Hancock fiksasyon testi ile akrozomal bütünlük açısından daha iyi sonuçlar vermiştir (p < 0.05). Sonuç olarak, Tris-yumurta sarısı sulandırıcısına %1.0 (h/h) ve %2.5 (h/v) çiçek balı ilavesiyle çözülme sonrası spermatolojik parametrelerde daha iyi sonuçlar kaydedildi.
{"title":"Effects of the addition of flower honey and pine honey to extenders on spermatological characteristics in ram semen","authors":"H. Yüksel, A. Eser, R. Arıcı, S. Yagcioglu, K. Ak","doi":"10.12681/jhvms.29579","DOIUrl":"https://doi.org/10.12681/jhvms.29579","url":null,"abstract":"Bu çalışmada, belirli oranlarda çiçek ve çam balının koç spermatının spermatolojik parametreleri üzerine etkileri araştırılmıştır. Tris-yumurta sarısı bazlı meni sulandırıcıya her bal türü için %1.0 (h/h) ve %2.5 (h/h) oranlarında çiçek ve çam balı eklenmiştir. Sperm toplama için beş Kıvircik koç kullanıldı. Toplanan spermler havuzlamanın ardından 5 eşit parçaya bölündü. %1.0 (h/h) ve %2.5 (h/v) çam balı ve %1.0 (h/v) ve %2.5 (h/h) çiçek balı oranlarında ve bal ilavesi yapılmadan iki aşamalı seyreltme yöntemi kullanılmıştır. Tris-yumurta sarısı bazlı meni genişleticiye. Numuneler daha sonra sıvı nitrojen buharında dondu ve sıvı nitrojen içinde saklandı. Semen canlılığı parametreleri (toplam ve progresif motilite, hız, kinetik hareket parametreleri ve mitokondriyal aktivite) ve morfolojik bütünlük parametreleri (Hancock fiksasyon testi, akış sitometrisi ve floresan boyama) dengeleme ve çözdürmeden sonra değerlendirildi. Çalışma boyunca on tekrarlı çalışma yapılmıştır.Sonuçlar çiçek balının çözülme sonrası canlılık parametreleri üzerinde özellikle %2,5 (h/h) oranında faydalı etkiye sahip olduğunu göstermiştir. Her oranda ve her çeşit bal içeren sulandırıcılar, çözüldükten sonra zar bütünlüğünü korumuştur. Tris-yumurta sarısı bazlı sulandırıcıya %1.0 (v/v) çiçek balı ilavesi, diğer gruplara kıyasla Hancock fiksasyon testi ile akrozomal bütünlük açısından daha iyi sonuçlar vermiştir (p < 0.05). Sonuç olarak, Tris-yumurta sarısı sulandırıcısına %1.0 (h/h) ve %2.5 (h/v) çiçek balı ilavesiyle çözülme sonrası spermatolojik parametrelerde daha iyi sonuçlar kaydedildi.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MG Jouybari, A. Sadeghi, GS Jouzani, M. Chamani, M. Aminafshar
The objective of this study was to evaluate the effect of the probiotic Bacillus subtilis supplementation on bacterial population, morphometry and mucin gene expression of intestine, and on immune response in Salmonella challenged broilers. Treatments were: 1) negative control (no probiotic- no challenging); 2) probiotic treated (no challenging); 3) positive control (no probiotic-salmonella challenging), and 4) Salmonella challenged chicks fed probiotic. Salmonella infection resulted in significant decrease in the relative weight of thymus. In challenged birds, dietary addition of probiotic increased (P<0.0.5) the relative weights of bursa and thymus. Population of lactic acid bacteria was significantly higher in probiotic treated groups as compared to negative control or challenged chickens. Challenged chickens had the lowest count of goblet cells and those received probiotic had the highest goblet cells count. The expression of mucin 2 gene was higher in group received dietary probiotic as compared with other treatments. Difference for gene expression was not found in challenged chickens and the negative control (P>0.05). In conclusion, the dietary inclusion of Bacillus subtilis could alleviate the negative effects of Salmonella infection on intestinal cells and bacterial population and could improve the growth and development of immune organs and function in infected broilers.
{"title":"Effects of Bacillus subtilis on the immune parameters, intestinal morphology and mucin gene expression in broilers exposed to Salmonella enterica challenge","authors":"MG Jouybari, A. Sadeghi, GS Jouzani, M. Chamani, M. Aminafshar","doi":"10.12681/jhvms.30558","DOIUrl":"https://doi.org/10.12681/jhvms.30558","url":null,"abstract":"The objective of this study was to evaluate the effect of the probiotic Bacillus subtilis supplementation on bacterial population, morphometry and mucin gene expression of intestine, and on immune response in Salmonella challenged broilers. Treatments were: 1) negative control (no probiotic- no challenging); 2) probiotic treated (no challenging); 3) positive control (no probiotic-salmonella challenging), and 4) Salmonella challenged chicks fed probiotic. Salmonella infection resulted in significant decrease in the relative weight of thymus. In challenged birds, dietary addition of probiotic increased (P<0.0.5) the relative weights of bursa and thymus. Population of lactic acid bacteria was significantly higher in probiotic treated groups as compared to negative control or challenged chickens. Challenged chickens had the lowest count of goblet cells and those received probiotic had the highest goblet cells count. The expression of mucin 2 gene was higher in group received dietary probiotic as compared with other treatments. Difference for gene expression was not found in challenged chickens and the negative control (P>0.05). In conclusion, the dietary inclusion of Bacillus subtilis could alleviate the negative effects of Salmonella infection on intestinal cells and bacterial population and could improve the growth and development of immune organs and function in infected broilers. \u0000 ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mast cells (MC)are unique members of immune system; their location and functions are of great importance in health and disease status. This study aims to evaluate MCs distribution and heterogenity in healthy and digital dermatitis lesions of dairy cattle for the first time. A total of 50 skin samples, 25 healthy and 25 with digital dermatitis (DD) lesions were sampled in Holstein-Friesian dairy cattle. All samples were stained with hematoxylin-eosin (H&E) for microscopic examination and also stained with Toluidine blue for MCs demonstration. Epidermal acanthosis and hyperkeratosis with dermal inflammatory cell infiltrations were in consistent with digital dermatitis lesions. Spirochetal agents were successfully demonstrated with Warthin-Starry staining in 22 out of 25 DD samples. Increased number of mast cell were observed in digital dermatitis samples when compared with healthy skin samples. The average number of intact MCs were 4.6 ± 2 and 9.3 ± 1 in healthy and digital dermatitis samples, respectively. Two-fold increase in the number of intact MCs in digital dermatitis samples was observed. Degranulated mast cell numbers in tissue sections were also higher in digital dermatitis samples and 4.25-fold increase was recorded in affected skin samples. Immunophenotype of MCs in skin samples were identified by immmunohistochemical stainings with anti-tryptase and chymase antibodies. Only tryptase positive MCs were observed in healthy and DD samples. In the statistical analysis, differences in the mean intact and degranulated MCs were found to be significant (mean intact: p<0.05, mean degranulated: p<0.01). Our results possibly suggest that MCs may have important roles in the pathogenesis of bovine digital dermatitis.
{"title":"Immunohistochemical Demonstration of Mast Cells in Bovine Papillomatous Digital Dermatitis of Holstein-Friesian Dairy Cattle","authors":"CG Ozguden-Akkoc, A. Akkoc","doi":"10.12681/jhvms.29927","DOIUrl":"https://doi.org/10.12681/jhvms.29927","url":null,"abstract":"Mast cells (MC)are unique members of immune system; their location and functions are of great importance in health and disease status. This study aims to evaluate MCs distribution and heterogenity in healthy and digital dermatitis lesions of dairy cattle for the first time. A total of 50 skin samples, 25 healthy and 25 with digital dermatitis (DD) lesions were sampled in Holstein-Friesian dairy cattle. All samples were stained with hematoxylin-eosin (H&E) for microscopic examination and also stained with Toluidine blue for MCs demonstration. Epidermal acanthosis and hyperkeratosis with dermal inflammatory cell infiltrations were in consistent with digital dermatitis lesions. Spirochetal agents were successfully demonstrated with Warthin-Starry staining in 22 out of 25 DD samples. Increased number of mast cell were observed in digital dermatitis samples when compared with healthy skin samples. The average number of intact MCs were 4.6 ± 2 and 9.3 ± 1 in healthy and digital dermatitis samples, respectively. Two-fold increase in the number of intact MCs in digital dermatitis samples was observed. Degranulated mast cell numbers in tissue sections were also higher in digital dermatitis samples and 4.25-fold increase was recorded in affected skin samples. Immunophenotype of MCs in skin samples were identified by immmunohistochemical stainings with anti-tryptase and chymase antibodies. Only tryptase positive MCs were observed in healthy and DD samples. In the statistical analysis, differences in the mean intact and degranulated MCs were found to be significant (mean intact: p<0.05, mean degranulated: p<0.01). Our results possibly suggest that MCs may have important roles in the pathogenesis of bovine digital dermatitis.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139627170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This experiment was carried out to investigate the effects of reduced dietary calcium (Ca) and non-phytate-phosphorus (NPP) levels at 2:1 constant ratio on performance, carcase parameters and bone mineralization of Ross-308 broilers. A total of 11400 one-day-old chicks were randomly allocated to five dietary treatments with twelve replicates and were fed starter diets including either recommended (control treatment, T1) or a 6.25% lower level of both Ca and NPP according to breeder’s recommendations during starter period (SP). After SP, control treatment (T1) continued to be provided through diets containing 0.87 and 0.78 % Ca for grower (GP) and finisher periods (FP), respectively, while 6.25 % reduced group was divided into 4 dietary treatments regarding extent of decrease in Ca and NPP levels and provided by diets containing 0.79;0.65 (T2), 0.79;0.60 (T3), 0.75;0.65 (T4), and 0.75;0.60 (T5) % Ca for GP and FP, respectively. After SP, moderate and even substantial reduction of Ca and NPP significantly decreased feed intake (FI) (quadratic, P<0.05), but obtained similar body weight gain (BWG) and FCR compared to the control (P>0.05). On the other hand, reduced dietary Ca through T1 to T5 significantly decreased the total Ca and NPP intake of broilers (linear and quadratic, P<0.01), and accompanied to significant linear and quadratic relationship between Ca intake and FCR and BWG respectively. It can be concluded that reducing Ca level down to 0.75 and 0.60 % in grower and finisher phase, respectively are possible without compromising the growth performance and bone mineralization of modern broilers.
{"title":"Effects of Lower Dietary Calcium and Phosphorus on Growth Performance and Bone Mineralizatıon of Broilers","authors":"N Ceylan, S Koca, İ Yavaş, AA Çenesiz","doi":"10.12681/jhvms.30761","DOIUrl":"https://doi.org/10.12681/jhvms.30761","url":null,"abstract":"This experiment was carried out to investigate the effects of reduced dietary calcium (Ca) and non-phytate-phosphorus (NPP) levels at 2:1 constant ratio on performance, carcase parameters and bone mineralization of Ross-308 broilers. A total of 11400 one-day-old chicks were randomly allocated to five dietary treatments with twelve replicates and were fed starter diets including either recommended (control treatment, T1) or a 6.25% lower level of both Ca and NPP according to breeder’s recommendations during starter period (SP). After SP, control treatment (T1) continued to be provided through diets containing 0.87 and 0.78 % Ca for grower (GP) and finisher periods (FP), respectively, while 6.25 % reduced group was divided into 4 dietary treatments regarding extent of decrease in Ca and NPP levels and provided by diets containing 0.79;0.65 (T2), 0.79;0.60 (T3), 0.75;0.65 (T4), and 0.75;0.60 (T5) % Ca for GP and FP, respectively. After SP, moderate and even substantial reduction of Ca and NPP significantly decreased feed intake (FI) (quadratic, P<0.05), but obtained similar body weight gain (BWG) and FCR compared to the control (P>0.05). On the other hand, reduced dietary Ca through T1 to T5 significantly decreased the total Ca and NPP intake of broilers (linear and quadratic, P<0.01), and accompanied to significant linear and quadratic relationship between Ca intake and FCR and BWG respectively. It can be concluded that reducing Ca level down to 0.75 and 0.60 % in grower and finisher phase, respectively are possible without compromising the growth performance and bone mineralization of modern broilers.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pyrethroids are synthetic insecticides which are being widely used to control fleas and ticks in household pets. However, their use extends to pest control as well. Products used worldwide to protect pets are in liquid form and comprise spot-ons, shampoos, and spray. Close attention should be paid to proper usage of such insecticides in pets, particularly in cats. As already indicated by many studies, accidental or improper usage of such products can trigger severe health problems even proving fatal in cats. The objective of the current paper is to undertake a retrospective study of a total number of 47 cases suffering from pyrethroid intoxication in cats across several clinics in Tirana, Albania during the period 2016-2020. Records from the veterinary clinics were closely scrutinized with only specific cases of pyrethroid intoxication being included for the purposes of this study. The main clinical signs which were noticed in 47 cats included symptoms such as tremor/muscle fasciculation (37 cats; 76.6%), seizures (18; 38.3%), hyperaesthesia (19; 40.43%), ptyalism (13; 27.66%), ataxia (11; 23.4%), mydriasis (10; 21.28%). Out of 47 cases, 7 or 14.9 % of the treated cats did not survive. The outcome was solid after rapid interventions in 31 (65.96%) cats with the exception of 2 cats which did not survive dispide receiving treatment. Whereas as many as 16 (34.04%) of cats, having being taken late to the clinics, survived upon receiving treatment, while as few as 5 cats (31.25%) died. Awareness campaign targeting pet owners, owner education, appropriate product labelling and consultations with veterinarians may help eliminate this problem in the future.
{"title":"Monitoring cases of pyrethroid intoxications in cats in Tirana region, Albania during 2016-2020","authors":"J Mavromati, B Mehmedi","doi":"10.12681/jhvms.30680","DOIUrl":"https://doi.org/10.12681/jhvms.30680","url":null,"abstract":"Pyrethroids are synthetic insecticides which are being widely used to control fleas and ticks in household pets. However, their use extends to pest control as well. Products used worldwide to protect pets are in liquid form and comprise spot-ons, shampoos, and spray. Close attention should be paid to proper usage of such insecticides in pets, particularly in cats. As already indicated by many studies, accidental or improper usage of such products can trigger severe health problems even proving fatal in cats. The objective of the current paper is to undertake a retrospective study of a total number of 47 cases suffering from pyrethroid intoxication in cats across several clinics in Tirana, Albania during the period 2016-2020. Records from the veterinary clinics were closely scrutinized with only specific cases of pyrethroid intoxication being included for the purposes of this study. The main clinical signs which were noticed in 47 cats included symptoms such as tremor/muscle fasciculation (37 cats; 76.6%), seizures (18; 38.3%), hyperaesthesia (19; 40.43%), ptyalism (13; 27.66%), ataxia (11; 23.4%), mydriasis (10; 21.28%). Out of 47 cases, 7 or 14.9 % of the treated cats did not survive. The outcome was solid after rapid interventions in 31 (65.96%) cats with the exception of 2 cats which did not survive dispide receiving treatment. Whereas as many as 16 (34.04%) of cats, having being taken late to the clinics, survived upon receiving treatment, while as few as 5 cats (31.25%) died. Awareness campaign targeting pet owners, owner education, appropriate product labelling and consultations with veterinarians may help eliminate this problem in the future.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thus, the aim of the current study was to investigate the genotypic dispersion of AA-NAT, SCD, PROP1, and IGFBP3 genes in the target population. Primarily, the genomic DNA was isolated from blood samples by the phenol/chloroform method. The genomic analyses were performed on a total of 160 Karacabey Merino (KM) sheep (n=80) and Kivircik (n=80). The PCR-RFLP method was used to determine the genotype of the SNPs. Deviation from Hardy–Weinberg equilibrium (HWE) and population genetic assays such as observed (Ho) and expected (He) heterozygosities, effective allele numbers (Ne), and the polymorphism information content (PIC) were calculated for each gene in the population. As a result, there was no deviation from HWE was determined except SCD and PROP1 gene in KM. The most frequent genotypes were found AA (66,25% and 87,50%) for AA-NAT gene, CC (93,75% and 91,25%) for SCD gene and CC genotype (70,00% and 56,25%) for PROP1 gene in KM and Kivircik sheep. All breeds were detected monomorphic in terms of the IGFBP3 gene. In conclusion, the other regions of the IGFBP3 gene which were determined as monomorphic in the studied flock could be researched. Moreover, further studies should be necessary for defining the effects of investigated genes on related traits in KM and Kivircik sheep in terms of polymorphic genes specified.
{"title":"Establishment of AA-NAT, SCD, PROP1, and IGFBP3 gene frequency distributions in Karacabey Merino and Kivircik Sheep Breeds","authors":"D Dinçel, Ö Çobanoğlu","doi":"10.12681/jhvms.30666","DOIUrl":"https://doi.org/10.12681/jhvms.30666","url":null,"abstract":"Thus, the aim of the current study was to investigate the genotypic dispersion of AA-NAT, SCD, PROP1, and IGFBP3 genes in the target population. Primarily, the genomic DNA was isolated from blood samples by the phenol/chloroform method. The genomic analyses were performed on a total of 160 Karacabey Merino (KM) sheep (n=80) and Kivircik (n=80). The PCR-RFLP method was used to determine the genotype of the SNPs. Deviation from Hardy–Weinberg equilibrium (HWE) and population genetic assays such as observed (Ho) and expected (He) heterozygosities, effective allele numbers (Ne), and the polymorphism information content (PIC) were calculated for each gene in the population. As a result, there was no deviation from HWE was determined except SCD and PROP1 gene in KM. The most frequent genotypes were found AA (66,25% and 87,50%) for AA-NAT gene, CC (93,75% and 91,25%) for SCD gene and CC genotype (70,00% and 56,25%) for PROP1 gene in KM and Kivircik sheep. All breeds were detected monomorphic in terms of the IGFBP3 gene. In conclusion, the other regions of the IGFBP3 gene which were determined as monomorphic in the studied flock could be researched. Moreover, further studies should be necessary for defining the effects of investigated genes on related traits in KM and Kivircik sheep in terms of polymorphic genes specified.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Zarić, S Cocoli, D Šarčević, S Vještica, R Prodanović, N Puvača, M Carić
The purpose of this review is to demonstrate the importance of monitoring microbiological quality in water for animals and humans, which relies primarily on coliforms, mostly Escherichia coli. Fecal coliforms, such as E. coli, are more specific indicators of fecal pollution. A fecal contamination detection can be performed not just in drinking water, but also in the environment. As a result of improved detection methods for E. coli, drinking water is becoming more reliable as a result of the use of E. coli as an indicator of fecal pollution. Drinking water contamination by feces is currently best detected by E. coli. Accordingly, temperate environments are more likely to have fecal coliforms than tropical environments, human and animal feces have high levels of E. coli in comparison to other fecal coliforms, and E. coli detection methods are more affordable, fast, sensitive, specific, and easy to use than other fecal coliforms.
{"title":"Escherichia coli as Microbiological Quality Water Indicator:A High Importance for Human and Animal Health","authors":"G Zarić, S Cocoli, D Šarčević, S Vještica, R Prodanović, N Puvača, M Carić","doi":"10.12681/jhvms.30878","DOIUrl":"https://doi.org/10.12681/jhvms.30878","url":null,"abstract":"The purpose of this review is to demonstrate the importance of monitoring microbiological quality in water for animals and humans, which relies primarily on coliforms, mostly Escherichia coli. Fecal coliforms, such as E. coli, are more specific indicators of fecal pollution. A fecal contamination detection can be performed not just in drinking water, but also in the environment. As a result of improved detection methods for E. coli, drinking water is becoming more reliable as a result of the use of E. coli as an indicator of fecal pollution. Drinking water contamination by feces is currently best detected by E. coli. Accordingly, temperate environments are more likely to have fecal coliforms than tropical environments, human and animal feces have high levels of E. coli in comparison to other fecal coliforms, and E. coli detection methods are more affordable, fast, sensitive, specific, and easy to use than other fecal coliforms.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to determine the effects of chitosan oligosaccharide on feed conversion ratio, some egg quality parameters, and some blood parameters in Japanise quails. Chitosan oligosaccharide (ChitO) was added to the drinking water of quails. A total of 192 (7 – 8 weeks old) Japanese quails (Coturnix coturnix Japonica) were divided into one control and 3 treatment groups, each consisting of 48 quails. Each group was divided into 6 replicates. While no additive was added to the control group, ChitO was added to the drinking water of the first, second, and third groups at the levels of 0.015, 0.030 and 0.045 g / L, respectively. They were given water and feed ad libitum. At the end of the study, no significant differences were observed between the control and treatment groups, except in their feed consumption and egg shell weight. In the first treatment group (given 0.015 g / L ChitO), it was observed that feed consumption decreased significantly compared to the control group; however, the difference between the other treatment groups was insignificant. The egg shell weight of the second treatment group (0.030 g / L ChitO) was found to be significantly higher (p < 0.05) than that of the control group. In general, consumption of chitosan oligosaccharide with drinking water tended to decrease feed consumption, without adversely affecting feed efficiency, and tended to increase eggshell weight.
本试验旨在研究壳寡糖对日本鹌鹑饲料系数、蛋品质及血液指标的影响。将低聚壳聚糖(ChitO)添加到鹌鹑的饮用水中。选取7 ~ 8周龄日本鹌鹑192只，分为1个对照组和3个治疗组，每组48只。每组分为6个重复。在对照组不添加添加剂的情况下，在第一组、第二组和第三组的饮用水中分别添加0.015、0.030和0.045 g / L的ChitO。他们得到了免费的水和饲料。试验结束时，除采食量和蛋壳重外，对照组与处理组之间无显著差异。第一处理组(给予0.015 g / L ChitO)，与对照组相比，采食量显著降低;然而，其他治疗组之间的差异不显著。第二处理组(0.030 g / L ChitO)的蛋壳重显著高于对照组(p <0.05)，显著高于对照组。总的来说，饮用低聚壳聚糖有降低饲料耗量的趋势，但对饲料效率没有不利影响，反而有增加蛋壳重的趋势。
{"title":"The effects of ad - libitum consumption of chitosan oligosaccharide with drinking water on laying quails","authors":"C Kiraz, KE Buğdayci","doi":"10.12681/jhvms.30916","DOIUrl":"https://doi.org/10.12681/jhvms.30916","url":null,"abstract":"The aim of this study was to determine the effects of chitosan oligosaccharide on feed conversion ratio, some egg quality parameters, and some blood parameters in Japanise quails. Chitosan oligosaccharide (ChitO) was added to the drinking water of quails. A total of 192 (7 – 8 weeks old) Japanese quails (Coturnix coturnix Japonica) were divided into one control and 3 treatment groups, each consisting of 48 quails. Each group was divided into 6 replicates. While no additive was added to the control group, ChitO was added to the drinking water of the first, second, and third groups at the levels of 0.015, 0.030 and 0.045 g / L, respectively. They were given water and feed ad libitum. At the end of the study, no significant differences were observed between the control and treatment groups, except in their feed consumption and egg shell weight. In the first treatment group (given 0.015 g / L ChitO), it was observed that feed consumption decreased significantly compared to the control group; however, the difference between the other treatment groups was insignificant. The egg shell weight of the second treatment group (0.030 g / L ChitO) was found to be significantly higher (p < 0.05) than that of the control group. In general, consumption of chitosan oligosaccharide with drinking water tended to decrease feed consumption, without adversely affecting feed efficiency, and tended to increase eggshell weight.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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