The objective of this study was to observe the effect of Mannan oligosaccharides (MOS), a prebiotic, in the replacement of the antibiotics in the broilers. For this purpose, two hundred broiler chicks were bought from a hatchery and allocated into five treatments having four replicates (10 birds in each). Birds were distributed into five groups; control, antibiotics (Zinc Bacitracin 10%), probiotics (Bacillus subtilis) and prebiotics (MOS), and a combination of probiotics and prebiotics groups. Five iso-nitrogenous and iso-caloric diets were prepared and offered to birds. Feed intake and body weight were recorded. At the end of the trial, birds were slaughtered to obtain carcass and gut health data. Data collected were examined by ANOVA under CRD and mean values were compared using Tukey’s HSD (Honestly significant difference) test. Weight gain was higher (P<0.05) in birds fed diet having probiotics and prebiotics in a combination form. Improved (P<0.05) FCR was recorded in birds fed diet having prebiotics alone and in combination with probiotics. The dressing percentage was higher (P<0.05) in birds fed diet having Probiotics + Prebiotics and control birds. Chest yield was higher (P<0.05) in birds fed having Probiotics + Prebiotics. In gut morphometric parameters, there was observed an increase in villus height, and a significant change of increase in villus surface area was seen. In conclusion, the addition of prebiotics in combination with probiotics, in feed, remarkably improved growth performance and carcass yield in commercial broilers.
{"title":"Prebiotic, probiotic, and antibiotic growth promoters use in commercial broilers: A comparative study","authors":"A Ali, AS Qureshi, S Rehan, F Deeba, M Usman","doi":"10.12681/jhvms.30488","DOIUrl":"https://doi.org/10.12681/jhvms.30488","url":null,"abstract":"The objective of this study was to observe the effect of Mannan oligosaccharides (MOS), a prebiotic, in the replacement of the antibiotics in the broilers. For this purpose, two hundred broiler chicks were bought from a hatchery and allocated into five treatments having four replicates (10 birds in each). Birds were distributed into five groups; control, antibiotics (Zinc Bacitracin 10%), probiotics (Bacillus subtilis) and prebiotics (MOS), and a combination of probiotics and prebiotics groups. Five iso-nitrogenous and iso-caloric diets were prepared and offered to birds. Feed intake and body weight were recorded. At the end of the trial, birds were slaughtered to obtain carcass and gut health data. Data collected were examined by ANOVA under CRD and mean values were compared using Tukey’s HSD (Honestly significant difference) test. Weight gain was higher (P<0.05) in birds fed diet having probiotics and prebiotics in a combination form. Improved (P<0.05) FCR was recorded in birds fed diet having prebiotics alone and in combination with probiotics. The dressing percentage was higher (P<0.05) in birds fed diet having Probiotics + Prebiotics and control birds. Chest yield was higher (P<0.05) in birds fed having Probiotics + Prebiotics. In gut morphometric parameters, there was observed an increase in villus height, and a significant change of increase in villus surface area was seen. In conclusion, the addition of prebiotics in combination with probiotics, in feed, remarkably improved growth performance and carcass yield in commercial broilers.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"272 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W Mahmoudnezhad, A Nobakht, Y Mehmannavaz, S Mahdavi
The study was carried out to determine the effects of different levels of methionine in Pre-starter diets on PPAR gene expression and immune function of broiler chickens. Methionine (Met) is necessary to achieve a fast growth rate in chickens. A total of 240 Ross 308 broilers were equally assigned to 8 treatments with 3 replicates. The treatments included T1: diet with 20% methionine less than Ross catalog recommendation. T2: Standard diet in accordance with the recommendations of the Ross catalog. T3, T4, T5, T6, T7 and T8: diets with 20, 40, 60, 80, 100 and 120 % methionine more than Ross catalog recommendation. The results showed that there was a significant difference in the expression of PPAR gene with the difference in diet methionine levels in the 8 groups. PPAR controls the expression of several genes involved in the proliferation and differentiation of adipose tissue cells. Gene expression in broiler chickens with methionine deficiency and excess may have compensated for this deficiency and excess in the birds. Also, the results indicated that increasing in the levels of methionine in Pre-starter diets of chickens the antibody production against ND increased significantly (P<0.05). Besides, the antibody production against IBD increased significantly (P<0.05). Furthermore, the antibody production against H9N1 not affected (P>0.05). Studies suggest that dietary protein deficiency reduces the concentration of most amino acids in plasma and compromises the immune system. Totally it is suggested that the high levels of Met in the diet maybe beneficial and it needs more studies.
{"title":"Effects of different levels of methionine in pre-starter diets on immunity function and DNA methylation in broilers","authors":"W Mahmoudnezhad, A Nobakht, Y Mehmannavaz, S Mahdavi","doi":"10.12681/jhvms.30501","DOIUrl":"https://doi.org/10.12681/jhvms.30501","url":null,"abstract":"The study was carried out to determine the effects of different levels of methionine in Pre-starter diets on PPAR gene expression and immune function of broiler chickens. Methionine (Met) is necessary to achieve a fast growth rate in chickens. A total of 240 Ross 308 broilers were equally assigned to 8 treatments with 3 replicates. The treatments included T1: diet with 20% methionine less than Ross catalog recommendation. T2: Standard diet in accordance with the recommendations of the Ross catalog. T3, T4, T5, T6, T7 and T8: diets with 20, 40, 60, 80, 100 and 120 % methionine more than Ross catalog recommendation. The results showed that there was a significant difference in the expression of PPAR gene with the difference in diet methionine levels in the 8 groups. PPAR controls the expression of several genes involved in the proliferation and differentiation of adipose tissue cells. Gene expression in broiler chickens with methionine deficiency and excess may have compensated for this deficiency and excess in the birds. Also, the results indicated that increasing in the levels of methionine in Pre-starter diets of chickens the antibody production against ND increased significantly (P<0.05). Besides, the antibody production against IBD increased significantly (P<0.05). Furthermore, the antibody production against H9N1 not affected (P>0.05). Studies suggest that dietary protein deficiency reduces the concentration of most amino acids in plasma and compromises the immune system. Totally it is suggested that the high levels of Met in the diet maybe beneficial and it needs more studies.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the effects of calcium oxalate (CaOx) crystal formation on the kidneys and the effect of alpha-lipoic acid (ALA) on this process were investigated in rats. The study consists of 6 groups with 10 rats in each group. Ethylene glycol (EG) and ammonium chloride (AC) (0.75% EG + 0.75% AC) were added to the drinking water of different groups for 7 or 14 days to induce nephrolithiasis. The effects of the CaOx on the kidneys and ALA (100 mg/kg/day/orally) on this process were investigated via histopathological, immunohistochemical, and biochemical methods. In the histopathological examination, EG+AC application for both 7 and 14 days caused crystal accumulation in the tubule lumens, cystically dilated tubules, and hydropic degeneration in the tubular epithelium. However, inflammatory cell infiltration was observed merely in 14 days. In the immunohistochemical examination, when EG+AC administration was applied for 14 days only, it caused expression of ED1, alpha smooth muscle actin (α-SMA), and vimentin in the tubulointerstitial areas. However, α-SMA and vimentin expression was not observed in tubular epithelial cells. Transforming growth factor beta-1 (TGF-β1) expression was also detected in the tubular epithelium and intertubular cells at 14 days. It was determined that ALA administration with EG+AC application reduced the crystal accumulation in the tubule lumens (p<0.001), the degeneration of the tubular epithelium (p<0.001), and the expression of TGF-β1. In addition, it was detected that ALA caused an increase in glutathione peroxidase (GPx) (p<0.001) and Catalase (CAT) (p>0.05) activities, which decreased with EG+AC application.
{"title":"The Effect of Alpha Lipoic Acid on Pathogenesis of Experimental Nephrolithiasis and Epithelial Mesenchymal Transition","authors":"T Kutlu, F Kazak, A Uyar","doi":"10.12681/jhvms.28802","DOIUrl":"https://doi.org/10.12681/jhvms.28802","url":null,"abstract":"In this study, the effects of calcium oxalate (CaOx) crystal formation on the kidneys and the effect of alpha-lipoic acid (ALA) on this process were investigated in rats. The study consists of 6 groups with 10 rats in each group. Ethylene glycol (EG) and ammonium chloride (AC) (0.75% EG + 0.75% AC) were added to the drinking water of different groups for 7 or 14 days to induce nephrolithiasis. The effects of the CaOx on the kidneys and ALA (100 mg/kg/day/orally) on this process were investigated via histopathological, immunohistochemical, and biochemical methods. In the histopathological examination, EG+AC application for both 7 and 14 days caused crystal accumulation in the tubule lumens, cystically dilated tubules, and hydropic degeneration in the tubular epithelium. However, inflammatory cell infiltration was observed merely in 14 days. In the immunohistochemical examination, when EG+AC administration was applied for 14 days only, it caused expression of ED1, alpha smooth muscle actin (α-SMA), and vimentin in the tubulointerstitial areas. However, α-SMA and vimentin expression was not observed in tubular epithelial cells. Transforming growth factor beta-1 (TGF-β1) expression was also detected in the tubular epithelium and intertubular cells at 14 days. It was determined that ALA administration with EG+AC application reduced the crystal accumulation in the tubule lumens (p<0.001), the degeneration of the tubular epithelium (p<0.001), and the expression of TGF-β1. In addition, it was detected that ALA caused an increase in glutathione peroxidase (GPx) (p<0.001) and Catalase (CAT) (p>0.05) activities, which decreased with EG+AC application.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L Spalević, N Zdravkovic, J Zutić, D Vojinović, V Milićević, N Jezdimirović, J Kureljušić, O Radanović
Poultry represent an important global reservoir of Salmonella. Poultry salmonellosis results in great economic losses, and some serovars also have zoonotic potential. Some poultry can be infected with Salmonella without showing clinical signs of the disease, but is, nevertheless, a significant source of spread of the infection. Clinical symptoms range from digestive problems (white diarrhea, malabsorption) to bacteremia and death. The aim of this study was to retrospectively show the presence of bacteria Salmonella enterica subspecies enterica of various serovars in poultry in the Belgrade epizootiological area in a period of six years (2014-2019). A total of 4580 samples were examined including: incubated eggs, dead chickens, broiler feces and laying hens with total of 207 positive samples. Salmonella was isolated by standard microbiological methods followed by serological typing. In the examined period, the prevalence of poultry salmonella was 4.52%. The highest number of examined samples was recorded in 2017 (879), and positive in 2018 (65), while the lowest prevalence was recorded during year 2016 with 7 positive samples. Of all isolated and serotyped Salmonella, the most commonly isolated serovar was S. Enteritidis (65.28%), followed by S. Infantis (21.30%), S. Mbandaka (6.02%), S. Senftenberg (3.24%), S. Typhimurium (1.85%), S. Agona (0.93%), S. Taksony (0.93%), and the least common is S. Tshiongwe (0.46%). The first report of serovars : S. Agona, S. Taksony and S. Tshiongwe have been during 2018. The highest number of positive specimens was found in laying hens faces (116 of 921 tested), broiler faces (73 of 1147), chickens carcasses (12 of 1443) and incubated eggs (6 of 1069). Complete eradication of Salmonella from production is a difficult goal because of the presence of a heterogeneous serovars pool and various sources of infection. Prevention is the best tool for controlling Salmonella: hygiene, biosecurity and where applicable - vaccination. It is a great responsibility of the poultry farmers to apply the existing standards and to improve the new ones.
{"title":"A retrospective study on the isolated strains of Salmonella in poultry","authors":"L Spalević, N Zdravkovic, J Zutić, D Vojinović, V Milićević, N Jezdimirović, J Kureljušić, O Radanović","doi":"10.12681/jhvms.25005","DOIUrl":"https://doi.org/10.12681/jhvms.25005","url":null,"abstract":"Poultry represent an important global reservoir of Salmonella. Poultry salmonellosis results in great economic losses, and some serovars also have zoonotic potential. Some poultry can be infected with Salmonella without showing clinical signs of the disease, but is, nevertheless, a significant source of spread of the infection. Clinical symptoms range from digestive problems (white diarrhea, malabsorption) to bacteremia and death. The aim of this study was to retrospectively show the presence of bacteria Salmonella enterica subspecies enterica of various serovars in poultry in the Belgrade epizootiological area in a period of six years (2014-2019). A total of 4580 samples were examined including: incubated eggs, dead chickens, broiler feces and laying hens with total of 207 positive samples. Salmonella was isolated by standard microbiological methods followed by serological typing. In the examined period, the prevalence of poultry salmonella was 4.52%. The highest number of examined samples was recorded in 2017 (879), and positive in 2018 (65), while the lowest prevalence was recorded during year 2016 with 7 positive samples. Of all isolated and serotyped Salmonella, the most commonly isolated serovar was S. Enteritidis (65.28%), followed by S. Infantis (21.30%), S. Mbandaka (6.02%), S. Senftenberg (3.24%), S. Typhimurium (1.85%), S. Agona (0.93%), S. Taksony (0.93%), and the least common is S. Tshiongwe (0.46%). The first report of serovars : S. Agona, S. Taksony and S. Tshiongwe have been during 2018. The highest number of positive specimens was found in laying hens faces (116 of 921 tested), broiler faces (73 of 1147), chickens carcasses (12 of 1443) and incubated eggs (6 of 1069). Complete eradication of Salmonella from production is a difficult goal because of the presence of a heterogeneous serovars pool and various sources of infection. Prevention is the best tool for controlling Salmonella: hygiene, biosecurity and where applicable - vaccination. It is a great responsibility of the poultry farmers to apply the existing standards and to improve the new ones.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to examine the polymorphic structures of the Myostatin (MSTN) gene locus in the native Morkaraman sheep, and to determine the distribution of genotype and allele frequencies. MSTN/HaeIII gene polymorphisms were identified in DNA isolated from blood samples from 262 Morkaraman sheep used in the study, using the PCR-RFLP method. According to the Hardy-Weinberg genetic balance test, it was observed that the distribution of genotype frequencies was not in balance (P<0.01) in the population studied. The MM, Mm and mm genotype frequencies of the MSTN gene in the population were determined as 11.1%, 62.6% and 26.3%, respectively, the frequencies of the M and m allele was 0.42 and 0.58 respectively. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values.While the FIS value was found as -0.312, the FIT value was determined as -0.303. The genotype and allele frequencies determined in terms of MSTN gene polymorphism were sufficient to reveal the genotype diversity of the breed.
{"title":"Determinatıon of the MSTN/HaeIII gene polymorphism in indigenous Morkaraman sheep","authors":"E Saygili, M Ozdemir","doi":"10.12681/jhvms.29799","DOIUrl":"https://doi.org/10.12681/jhvms.29799","url":null,"abstract":"The aim of this study was to examine the polymorphic structures of the Myostatin (MSTN) gene locus in the native Morkaraman sheep, and to determine the distribution of genotype and allele frequencies. MSTN/HaeIII gene polymorphisms were identified in DNA isolated from blood samples from 262 Morkaraman sheep used in the study, using the PCR-RFLP method. According to the Hardy-Weinberg genetic balance test, it was observed that the distribution of genotype frequencies was not in balance (P<0.01) in the population studied. The MM, Mm and mm genotype frequencies of the MSTN gene in the population were determined as 11.1%, 62.6% and 26.3%, respectively, the frequencies of the M and m allele was 0.42 and 0.58 respectively. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values.While the FIS value was found as -0.312, the FIT value was determined as -0.303. The genotype and allele frequencies determined in terms of MSTN gene polymorphism were sufficient to reveal the genotype diversity of the breed.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135972225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was performed to investigate the effect of dietary aspirin (A) on some performance traits in broilers using a completely randomized design, 120 one-day-old male broiler chicks Ross 308, three levels of aspirin (0, 50, and 100 mg/kg), in 4 replicates, each including 10 chicks, during 42 days. The effects of different levels of aspirin, added to a basal diet, on performance, carcass characteristics, and digestive organs of chicks were investigated. Data analysis was performed by SAS statistical software and the comparison of the means with Duncan's test. The results showed that the chickens fed by a diet containing 100 mg/kg of aspirin had the highest feed intake and weight gain and the best feed conversion ratio compared to the other treatments. Also, the lowest production cost and the best European factor were related to treatment A100 (P<0.05). In addition, the use of the same level of aspirin resulted in a significant increase in some carcass properties and a decrease in ventricular fat compared to the control (P<0.05). So, based on the results of the present study, the use of 100 mg/kg aspirin in the diet of broilers is recommendable to improve some performance parameters.
{"title":"Influences of dietary aspirin supplementation on growth performance, carcass characteristics and gastrointestinal organs of broilers","authors":"M. Tavakoli, M. Bouyeh, A. Seidavi","doi":"10.12681/jhvms.27696","DOIUrl":"https://doi.org/10.12681/jhvms.27696","url":null,"abstract":"This study was performed to investigate the effect of dietary aspirin (A) on some performance traits in broilers using a completely randomized design, 120 one-day-old male broiler chicks Ross 308, three levels of aspirin (0, 50, and 100 mg/kg), in 4 replicates, each including 10 chicks, during 42 days. The effects of different levels of aspirin, added to a basal diet, on performance, carcass characteristics, and digestive organs of chicks were investigated. Data analysis was performed by SAS statistical software and the comparison of the means with Duncan's test. The results showed that the chickens fed by a diet containing 100 mg/kg of aspirin had the highest feed intake and weight gain and the best feed conversion ratio compared to the other treatments. Also, the lowest production cost and the best European factor were related to treatment A100 (P<0.05). In addition, the use of the same level of aspirin resulted in a significant increase in some carcass properties and a decrease in ventricular fat compared to the control (P<0.05). So, based on the results of the present study, the use of 100 mg/kg aspirin in the diet of broilers is recommendable to improve some performance parameters.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45965911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.
{"title":"Genetic polymorphisms of MASP2, TG5, and DQA1 genes in Holstein and Brown Swiss dairy cows","authors":"A. El Zeer, M. Fouda, R. Darwish, A. Ateya","doi":"10.12681/jhvms.29524","DOIUrl":"https://doi.org/10.12681/jhvms.29524","url":null,"abstract":"The objective of this study was to investigate polymorphisms of MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, and Brown Swiss dairy cows (35 cows each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of 305-bp of MASP2, 545-bp of TG5, and 373-bp of DQA1 genes. DNA sequencing assessment elaborated nucleotide sequence variations in forms of single nucleotide polymorphisms (SNPs) between two breeds. The SNPs identified in the investigated genes for Holstein breed were A46G in MASP2 gene and C371T in TG5 gene. A characteristic T53C SNP was also reported for Brown Swiss breed in DQA1 gene. Chi-square revealed a significant variation in distribution of all identified SNPs (P < 0.001). Consequently, identified SNPs could be used as candidates and a reference guide for efficient characterization of Holsteins, and Brown Swiss breeds; therefore, developing a marker assisted selection (MAS) for production and immunity traits in breeds of dairy cattle.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42702168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Akkaya, FZ Evci, ZA Küpeli, Ç. Kandemir, T. Taşkin, N. Koşum
In this case, it was aimed to report the clinical, hematological and pathological analysis results of a lamb born as infected with border disease virus (BDV). Severe central nervous system findings such as standing inability, continuous teeth grinding, muscle fasciculations in the head, ear and tail, hair loss around the eyes, lips and ears, abnormal appearance of the fleece, ataxia/rhythmic tremor and incoordination were detected in the case of lamb infected with BDV. The presence of BDV antibody and the levels of Triiodothyronine (T3) and Thyroxine (T4) were investigated in the serum samples for definite diagnosis. The tissue samples obtained from the brain and cerebellum were evaluated histopathologically and diagnosis of BDV was confirmed by necroscopic findings.
{"title":"Border Disease In A Lamb: A Case Report","authors":"F. Akkaya, FZ Evci, ZA Küpeli, Ç. Kandemir, T. Taşkin, N. Koşum","doi":"10.12681/jhvms.28216","DOIUrl":"https://doi.org/10.12681/jhvms.28216","url":null,"abstract":"In this case, it was aimed to report the clinical, hematological and pathological analysis results of a lamb born as infected with border disease virus (BDV). Severe central nervous system findings such as standing inability, continuous teeth grinding, muscle fasciculations in the head, ear and tail, hair loss around the eyes, lips and ears, abnormal appearance of the fleece, ataxia/rhythmic tremor and incoordination were detected in the case of lamb infected with BDV. The presence of BDV antibody and the levels of Triiodothyronine (T3) and Thyroxine (T4) were investigated in the serum samples for definite diagnosis. The tissue samples obtained from the brain and cerebellum were evaluated histopathologically and diagnosis of BDV was confirmed by necroscopic findings.\u0000 \u0000 ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48017684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was carried out to determine the virulence gene profiles associated with Salmonella subsp. enterica strains recovered from cattle and sheep samples by polymerase chain reaction (PCR). A total of 84 Salmonella isolates belonging to 10 serovars were screened for presence of 8 different virulence genes (invA, pefA, sefA, pipB, sseC, ssaP, spvC and iroB) using their specific primers. Different distribution patterns of these genes were noticed amongst the isolates. While the iroB gene was the most identified (n= 65, 77.4%), the sefA gene was not detected in all tested strains. pefA and spvC genes were detected in S. Typhimurium only with a rate of 3.6%. The four other genes were found to be present in 61.9% each. Occurrence of virulence genes did not vary significantly (P> 0.05) by serovar or animal species, neither by type of sample. The presence of all screened genes was significantly associated with resistance to at least one antibiotic from different classes except quinolones. These results show that transmissible pathogenic Salmonella strains are circulating in slaughterhouses in Algeria.
{"title":"Distribution of Salmonella virulence factors originated from sheep and cattle in Algerian slaughterhouses","authors":"S. Nouichi, L. Mezali, TM Hamdi","doi":"10.12681/jhvms.29148","DOIUrl":"https://doi.org/10.12681/jhvms.29148","url":null,"abstract":"The present study was carried out to determine the virulence gene profiles associated with Salmonella subsp. enterica strains recovered from cattle and sheep samples by polymerase chain reaction (PCR). A total of 84 Salmonella isolates belonging to 10 serovars were screened for presence of 8 different virulence genes (invA, pefA, sefA, pipB, sseC, ssaP, spvC and iroB) using their specific primers. Different distribution patterns of these genes were noticed amongst the isolates. While the iroB gene was the most identified (n= 65, 77.4%), the sefA gene was not detected in all tested strains. pefA and spvC genes were detected in S. Typhimurium only with a rate of 3.6%. The four other genes were found to be present in 61.9% each. Occurrence of virulence genes did not vary significantly (P> 0.05) by serovar or animal species, neither by type of sample. The presence of all screened genes was significantly associated with resistance to at least one antibiotic from different classes except quinolones. These results show that transmissible pathogenic Salmonella strains are circulating in slaughterhouses in Algeria.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48824787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Endometritis is one of the most common disorders that negatively influence reproductive performance in dairy cows. The purpose of this study was to determine the predictive factors of the clinical cure of endometritis. A total of 700 Holstein dairy cows were examined for signs of clinical endometritis between 30 and 40 days in milk (the first examination in the time of clean test). A total of 131 cows with clinical endometritis were assigned for treatment. Cytological examination, reagent test strips, and characteristics of reproductive organs were evaluated at the time of treatment (the first examination) and two weeks after the treatment (the second examination: for evaluation of response to treatment of endometritis). After evaluation of treatment, cows were grouped into the responsive (n=56) and unresponsive (n=75) to treatment. Percentages of non-degenerated neutrophil (NDN), degenerated neutrophil (DN), total neutrophils and leukocyte esterase activity were significantly higher (P<0.05) in the unresponsive cows than those of responsive ones to treatment at the second examinations. Moreover, the nitrate test was significantly higher in the unresponsive group than the responsive group at the first examination. However, protein concentration and percentages of lymphocyte cells were significantly higher in the unresponsive animals than the responsive cows at the first and second stages of examination (P<0.05). Increasing of lymphocytes percentage, protein concentration, and nitrate test at the first stage of examination in the unresponsive cows showed more sever of inflammatory conditions and uterine infection in the unresponsive cows. Evaluation of these factors can predict the clinical cure of endometritis in dairy cows.
{"title":"Determination of predictive factors for the clinical cure rate of endometritis in Holstein dairy cows","authors":"A. Hajibemani, A. Mirzaei","doi":"10.12681/jhvms.28687","DOIUrl":"https://doi.org/10.12681/jhvms.28687","url":null,"abstract":"Endometritis is one of the most common disorders that negatively influence reproductive performance in dairy cows. The purpose of this study was to determine the predictive factors of the clinical cure of endometritis. A total of 700 Holstein dairy cows were examined for signs of clinical endometritis between 30 and 40 days in milk (the first examination in the time of clean test). A total of 131 cows with clinical endometritis were assigned for treatment. Cytological examination, reagent test strips, and characteristics of reproductive organs were evaluated at the time of treatment (the first examination) and two weeks after the treatment (the second examination: for evaluation of response to treatment of endometritis). After evaluation of treatment, cows were grouped into the responsive (n=56) and unresponsive (n=75) to treatment. Percentages of non-degenerated neutrophil (NDN), degenerated neutrophil (DN), total neutrophils and leukocyte esterase activity were significantly higher (P<0.05) in the unresponsive cows than those of responsive ones to treatment at the second examinations. Moreover, the nitrate test was significantly higher in the unresponsive group than the responsive group at the first examination. However, protein concentration and percentages of lymphocyte cells were significantly higher in the unresponsive animals than the responsive cows at the first and second stages of examination (P<0.05). Increasing of lymphocytes percentage, protein concentration, and nitrate test at the first stage of examination in the unresponsive cows showed more sever of inflammatory conditions and uterine infection in the unresponsive cows. Evaluation of these factors can predict the clinical cure of endometritis in dairy cows.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49472081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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