Abortion in sheep and goats is one of the most important reproductive problems that affect small ruminants breeding. Non-infectious factors and infectious agents such as bacterial, viral, mycotic and parasitic infectious agents can cause abortion in sheep and goats. A high rate of abortion (43.75%) was observed in two sheep flocks in Niğde Province in Turkey during the lambing season in 2016. To determine the reason of abortion, aborted sheep foetuses (n = 3) and EDTA whole blood samples (n = 3) from mother of the foetuses were collected from two sheep flocks. Aborted foetuses and buffy coat cells of the EDTA whole blood samples were used for total nucleic acid extraction. Extracted nucleic acids were analysed for akabane virus (AKAV), bluetongue virus (BTV), peste des petits ruminants virus (PPRV), pestiviruses, Schmallenberg virus (SBV), Brucella spp., Chlamydophila abortus (C. abortus), Coxiella burnetii and Listeria monocytogenes. Border disease virus (BDV) RNA and C. abortus DNA were detected in three aborted sheep foetuses whereas other investigated infectious agents were not detected. Additionally, EDTA whole blood samples from mother of the foetuses were also found BDV positive. To the best of my knowledge, this is the first report on BDV and C. abortus co-infection in aborted sheep foetuses.
{"title":"Border disease virus and chlamydophila abortus co-infection in aborted sheep foetuses","authors":"M Şevik","doi":"10.12681/jhvms.30570","DOIUrl":"https://doi.org/10.12681/jhvms.30570","url":null,"abstract":"Abortion in sheep and goats is one of the most important reproductive problems that affect small ruminants breeding. Non-infectious factors and infectious agents such as bacterial, viral, mycotic and parasitic infectious agents can cause abortion in sheep and goats. A high rate of abortion (43.75%) was observed in two sheep flocks in Niğde Province in Turkey during the lambing season in 2016. To determine the reason of abortion, aborted sheep foetuses (n = 3) and EDTA whole blood samples (n = 3) from mother of the foetuses were collected from two sheep flocks. Aborted foetuses and buffy coat cells of the EDTA whole blood samples were used for total nucleic acid extraction. Extracted nucleic acids were analysed for akabane virus (AKAV), bluetongue virus (BTV), peste des petits ruminants virus (PPRV), pestiviruses, Schmallenberg virus (SBV), Brucella spp., Chlamydophila abortus (C. abortus), Coxiella burnetii and Listeria monocytogenes. Border disease virus (BDV) RNA and C. abortus DNA were detected in three aborted sheep foetuses whereas other investigated infectious agents were not detected. Additionally, EDTA whole blood samples from mother of the foetuses were also found BDV positive. To the best of my knowledge, this is the first report on BDV and C. abortus co-infection in aborted sheep foetuses.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feline panleukopenia (FP) virus, which is closely related to canine parvovirus, is a fatal viral disease that affects mitotically active tissues such as intestinal cells, lymphoid tissue and bone marrow in cats of all ages and causes a wide variety of clinical findings. Despite its high incidence, there is still a need for studies on the effectiveness of demographic, routine clinical and hemogram data on the diagnosis of FP, which has not been investigated as much as canine parvoviral infection. The Panleukopenia Group of the study consisted of 50 naturally infected cats with panleukopenia, and the Control Group consisted of 10 healthy cats of similar age and body weight. Information on sex, age, body weight, breed and origin of all cats was recorded. Venous blood samples were obtained from the cats eligible for inclusion in the study, and the diagnostic efficacy of clinical examination findings and demographic data along with hemogram parameters were investigated. Most of the Panleukopenia Group cats were indoor and were bought from a breeder. The most prominent clinical finding of the diseased cats was loss of appetite. This was followed by dehydration, stagnation, depression, vomiting, diarrhea and ocular discharge. In clinical examination, respiratory rate, pulse and body temperature values were higher in the Panleukopenia Group (p< 0.017). As a result of hemogram analysis, it was determined that WBC, lymphocyte, monocytes, granulocytes, RBC, Hct, RDW, Hb and THR levels were lower in the Panleukopenia Group (p< 0.040). As a result of the ROC analysis, it was determined that from clinical examination parameters, respiratory rate had excellent, pulse and body temperature had good; from hemogram parameters, WBC and granulocyte had outstanding, lymphocyte and RDW had excellent, monocytes, Hct and THR had good, RBC and Hb had acceptable diagnostic performances. As a result, it was concluded that demographic data and clinical findings along with abnormal leukograms such as leukopenia, lymphopenia and granulocytopenia and abnormal hemogram patterns such as anemia and thrombocytopenia may be helpful in the diagnosis of FP in triage and in cases where antibodies bind to viral epitopes resulting in false negatives.
{"title":"Diagnostic Performances of Clinical and Hematological Parameters in Cats Naturally Infected with Feline PanleukopeniaVirus","authors":"E Gülersoy, C Balıkçı, BB Erol, A Şahan, İ Günal","doi":"10.12681/jhvms.30721","DOIUrl":"https://doi.org/10.12681/jhvms.30721","url":null,"abstract":"Feline panleukopenia (FP) virus, which is closely related to canine parvovirus, is a fatal viral disease that affects mitotically active tissues such as intestinal cells, lymphoid tissue and bone marrow in cats of all ages and causes a wide variety of clinical findings. Despite its high incidence, there is still a need for studies on the effectiveness of demographic, routine clinical and hemogram data on the diagnosis of FP, which has not been investigated as much as canine parvoviral infection. The Panleukopenia Group of the study consisted of 50 naturally infected cats with panleukopenia, and the Control Group consisted of 10 healthy cats of similar age and body weight. Information on sex, age, body weight, breed and origin of all cats was recorded. Venous blood samples were obtained from the cats eligible for inclusion in the study, and the diagnostic efficacy of clinical examination findings and demographic data along with hemogram parameters were investigated. Most of the Panleukopenia Group cats were indoor and were bought from a breeder. The most prominent clinical finding of the diseased cats was loss of appetite. This was followed by dehydration, stagnation, depression, vomiting, diarrhea and ocular discharge. In clinical examination, respiratory rate, pulse and body temperature values were higher in the Panleukopenia Group (p< 0.017). As a result of hemogram analysis, it was determined that WBC, lymphocyte, monocytes, granulocytes, RBC, Hct, RDW, Hb and THR levels were lower in the Panleukopenia Group (p< 0.040). As a result of the ROC analysis, it was determined that from clinical examination parameters, respiratory rate had excellent, pulse and body temperature had good; from hemogram parameters, WBC and granulocyte had outstanding, lymphocyte and RDW had excellent, monocytes, Hct and THR had good, RBC and Hb had acceptable diagnostic performances. As a result, it was concluded that demographic data and clinical findings along with abnormal leukograms such as leukopenia, lymphopenia and granulocytopenia and abnormal hemogram patterns such as anemia and thrombocytopenia may be helpful in the diagnosis of FP in triage and in cases where antibodies bind to viral epitopes resulting in false negatives.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In Turkey, water buffalo husbandry is conducted generally by using the traditional methods in shelters designed for cattle. The present study reports the data of the items of a questionnaire, which was conducted with 122 water buffalo breeders which are located in Central, Şarkışla, and Suşehri districts of Sivas province, regarding the structural and technical aspects of shelters such as shelter location, shelter type, building material, ventilation, and lighting. It was determined that, among the water buffalo breeders in Sivas province, the shelters were generally (73.5%) located nearby the house, that the building materials used in construction of the shelters were stone in 55.4%, concrete in 21.5% and briquette in 16.5% (p<0.0001), and that the floor materials used were concrete in 99.2% (p<0.05), whereas the roofing material was sheet metal/eternit in 80% (p<0.0001). It was found that water buffalos and cattle were held together in closed tethered systems (95.8%) and the space needs of animals were met by using chains having 40-60cm in length (63.1%). In conclusion, considering the animal welfare and behaviors, it was concluded that the infrastructural improvements to ensure the standardization in water buffalo shelters should be prioritized, that the old shelters should be re-organized, and that the watering systems in shelters should be analyzed.
{"title":"Examining the Structural and Technical Characteristics of the Water Buffalo Farms in Sivas Province from the Perspective of Animal Welfare","authors":"G Özdemir","doi":"10.12681/jhvms.30720","DOIUrl":"https://doi.org/10.12681/jhvms.30720","url":null,"abstract":"In Turkey, water buffalo husbandry is conducted generally by using the traditional methods in shelters designed for cattle. The present study reports the data of the items of a questionnaire, which was conducted with 122 water buffalo breeders which are located in Central, Şarkışla, and Suşehri districts of Sivas province, regarding the structural and technical aspects of shelters such as shelter location, shelter type, building material, ventilation, and lighting. It was determined that, among the water buffalo breeders in Sivas province, the shelters were generally (73.5%) located nearby the house, that the building materials used in construction of the shelters were stone in 55.4%, concrete in 21.5% and briquette in 16.5% (p<0.0001), and that the floor materials used were concrete in 99.2% (p<0.05), whereas the roofing material was sheet metal/eternit in 80% (p<0.0001). It was found that water buffalos and cattle were held together in closed tethered systems (95.8%) and the space needs of animals were met by using chains having 40-60cm in length (63.1%). In conclusion, considering the animal welfare and behaviors, it was concluded that the infrastructural improvements to ensure the standardization in water buffalo shelters should be prioritized, that the old shelters should be re-organized, and that the watering systems in shelters should be analyzed.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tuberculosis (TB) is a contagious and zoonotic disease that adversely affects human and animal health, caused by the formation of tubercules in a caseous character in the lungs, other tissues and organs. The causative of the disease is Mycobacterium bovis. An increase or decrease in acute phase protein (APP) levels is observed in various bacterial, viral and parasitic diseases in cattle. Therefore, in this study, it was aimed to determine the changes in the levels of AFPs in cattle infected with M. bovis. In the study, 26 tuberculosis suspected and 10 healthy cattle blood serums collected from various enterprises in Samsun were used. Rose bengal plate test and complement fixation test were applied in serum by taking blood into heparin containing tubes from vena jugularis of cattle. Whole blood samples were subjected to gamma interferon ELISA test. Haptoglobin (Hp), serum amyloid A (SAA), albumin and total protein (TP) levels, which are among the acute phase proteins, were determined in the blood serums taken from the groups. As a result of the analysis, when the AFP levels of the cattle infected with M. bovis and the cattle in the healthy group were compared, it was determined that haptoglobin (p < 0.01), serum amyloid A (p < 0.01) and total protein (p < 0.01) levels, which are AFPs increased statistically significantly compared to the healthy group, while the albümin (p > 0.05) level decreased, but there was no statistically significant difference.
Keywords: acute phase protein (APP); cattle; mycobacterium bovis; tuberculosis (TB),
{"title":"Investigation of Acute Phase Proteins in Cattle Infected with Mycobacterium bovis","authors":"S Çenesiz, B Şahin, RK Akpinar, Y Kiliçoğlu","doi":"10.12681/jhvms.30671","DOIUrl":"https://doi.org/10.12681/jhvms.30671","url":null,"abstract":"Tuberculosis (TB) is a contagious and zoonotic disease that adversely affects human and animal health, caused by the formation of tubercules in a caseous character in the lungs, other tissues and organs. The causative of the disease is Mycobacterium bovis. An increase or decrease in acute phase protein (APP) levels is observed in various bacterial, viral and parasitic diseases in cattle. Therefore, in this study, it was aimed to determine the changes in the levels of AFPs in cattle infected with M. bovis. In the study, 26 tuberculosis suspected and 10 healthy cattle blood serums collected from various enterprises in Samsun were used. Rose bengal plate test and complement fixation test were applied in serum by taking blood into heparin containing tubes from vena jugularis of cattle. Whole blood samples were subjected to gamma interferon ELISA test. Haptoglobin (Hp), serum amyloid A (SAA), albumin and total protein (TP) levels, which are among the acute phase proteins, were determined in the blood serums taken from the groups. As a result of the analysis, when the AFP levels of the cattle infected with M. bovis and the cattle in the healthy group were compared, it was determined that haptoglobin (p < 0.01), serum amyloid A (p < 0.01) and total protein (p < 0.01) levels, which are AFPs increased statistically significantly compared to the healthy group, while the albümin (p > 0.05) level decreased, but there was no statistically significant difference.
 Keywords: acute phase protein (APP); cattle; mycobacterium bovis; tuberculosis (TB),","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aseel birds are known for their hardiness, thermotolerance, pugnacity, and robustness. However, they are highly susceptible to Avian Influenza (AI) and Newcastle Disease (ND) viruses which cause huge mortalities. Present study was designed to identify polymorphisms in exon-14 of Mx1, and 5’UTR & intron-7 of ROBO2 gene associated with antibody response to AIV (H9N2) and ND virus respectively in adult Aseel birds. After screening of 500 birds for HI-based antibody response against both viruses, two separate set of 40 birds, one for each virus, were selected on the basis of maximum divergence in their antibody response. The sequence data of exon-14 of Mx1 gene revealed 3 SNP, and that of ROBO2 gene showed 2 SNPs in intron-7 and 1 in 5’UTR. The genotypic frequencies of identified variants were tested for goodness-of-fit and only Mx_810 showed significant difference. The results of association analysis revealed a non-synonymous SNP (G>A), Mx1_567, significantly associated with pre- (P<0.01) and post-vaccination (P<0.01) antibody response against AIV. The LSD results showed that homozygous mutant (AA), and heterozygous (AG) genotypes had significantly greater values of pre- and post-vaccination antibody response compared with wild-type genotype. However, no SNP in the ROBO2 gene was found significantly associated with antibody response.
飞禽以其耐寒、耐热、好斗和健壮而闻名。然而，它们对导致大量死亡的禽流感(AI)和新城疫(ND)病毒非常敏感。本研究旨在鉴定Mx1外显子14的多态性，以及5'UTR &成年雏鸟对AIV (H9N2)和ND病毒抗体应答相关的ROBO2基因内含子-7在筛选了500只鸟对这两种病毒的基于hiv的抗体反应后，根据抗体反应的最大差异，选择了两组40只鸟，每种病毒一种。Mx1基因外显子14的序列数据显示3个SNP, ROBO2基因的序列数据显示7内含子2个SNP, 5'UTR 1个SNP。对所鉴定变异的基因型频率进行拟合优度检验，只有Mx_810存在显著差异。关联分析结果显示，非同义SNP (G> a) Mx1_567与接种前(P<0.01)和接种后(P<0.01)抗AIV抗体应答显著相关。LSD结果显示，纯合子突变型(AA)和杂合子基因型(AG)在疫苗接种前和接种后的抗体应答值显著高于野生型基因型。然而，没有发现ROBO2基因的SNP与抗体反应显著相关。
{"title":"Identification of single nucleotide variant in Mx1 gene associated with antibody response to Avian Influenza virus in Aseel chickens","authors":"R Majeed, I Zahoor, A Anjum, M Ali, A Basheer","doi":"10.12681/jhvms.30940","DOIUrl":"https://doi.org/10.12681/jhvms.30940","url":null,"abstract":"Aseel birds are known for their hardiness, thermotolerance, pugnacity, and robustness. However, they are highly susceptible to Avian Influenza (AI) and Newcastle Disease (ND) viruses which cause huge mortalities. Present study was designed to identify polymorphisms in exon-14 of Mx1, and 5’UTR & intron-7 of ROBO2 gene associated with antibody response to AIV (H9N2) and ND virus respectively in adult Aseel birds. After screening of 500 birds for HI-based antibody response against both viruses, two separate set of 40 birds, one for each virus, were selected on the basis of maximum divergence in their antibody response. The sequence data of exon-14 of Mx1 gene revealed 3 SNP, and that of ROBO2 gene showed 2 SNPs in intron-7 and 1 in 5’UTR. The genotypic frequencies of identified variants were tested for goodness-of-fit and only Mx_810 showed significant difference. The results of association analysis revealed a non-synonymous SNP (G>A), Mx1_567, significantly associated with pre- (P<0.01) and post-vaccination (P<0.01) antibody response against AIV. The LSD results showed that homozygous mutant (AA), and heterozygous (AG) genotypes had significantly greater values of pre- and post-vaccination antibody response compared with wild-type genotype. However, no SNP in the ROBO2 gene was found significantly associated with antibody response.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linguatuliasis is caused by the Arthropod parasite Linguatula serrata (family: Linguatulidae) a parasite with worldwide distribution and zoonotic importance, especially in warm subtropical and temperate regions. Dogs, cats, foxes, jackals, and other carnivores are the final hosts of this Arthropod while any mammal could be a potential intermediate host of this parasite. Adults L. serrata live in the nasopharyngeal region and lower respiratory system, embed their body into the mucosa of nasal pharyngeal cavities. A two year old spayed female dog of undetermined breed, weighting 29 kg that lives in a mountain village called Sella (Prefecture of Achaia, Greece) in a semi-free form, was presented in a veterinary clinic of Patra with history of progressive cough and sneezing, which started about 2 weeks earlier. Along with the dog, the owner brought a parasite that was expelled from the dog’s nose during the intense cough and sneezing the same day. Macroscopic morphological aspects of the parasite, in the laboratory under stereoscope, revealed an arthropod 3,8cm in length identified as L. serrata, also commonly called tongue worm. The dog was treated with Macrocyclice lactone and lavage of nasal cavities. Humans can become infected in two ways: either as an intermediate host (visceral linguatuliasis) or as an accidental final host (nasopharyngeal linguatuliasis), usually as a result of consumption of raw or poorly cooked meat of an infected intermediate host.
{"title":"Management of Linguatula serrata infestation in a dog from Greece: a case report","authors":"M Lefkaditis, I Zapantes, A Giannouli","doi":"10.12681/jhvms.30904","DOIUrl":"https://doi.org/10.12681/jhvms.30904","url":null,"abstract":"Linguatuliasis is caused by the Arthropod parasite Linguatula serrata (family: Linguatulidae) a parasite with worldwide distribution and zoonotic importance, especially in warm subtropical and temperate regions. Dogs, cats, foxes, jackals, and other carnivores are the final hosts of this Arthropod while any mammal could be a potential intermediate host of this parasite. Adults L. serrata live in the nasopharyngeal region and lower respiratory system, embed their body into the mucosa of nasal pharyngeal cavities. A two year old spayed female dog of undetermined breed, weighting 29 kg that lives in a mountain village called Sella (Prefecture of Achaia, Greece) in a semi-free form, was presented in a veterinary clinic of Patra with history of progressive cough and sneezing, which started about 2 weeks earlier. Along with the dog, the owner brought a parasite that was expelled from the dog’s nose during the intense cough and sneezing the same day. Macroscopic morphological aspects of the parasite, in the laboratory under stereoscope, revealed an arthropod 3,8cm in length identified as L. serrata, also commonly called tongue worm. The dog was treated with Macrocyclice lactone and lavage of nasal cavities. Humans can become infected in two ways: either as an intermediate host (visceral linguatuliasis) or as an accidental final host (nasopharyngeal linguatuliasis), usually as a result of consumption of raw or poorly cooked meat of an infected intermediate host.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TA Vijayalingam, NV Rajesh, S Vairamuthu, M Boopathy Raja, NK Sudeep Kumar
The present study was conducted to evaluate the dietary effects of two seaweeds (Sargassum wightii and Gracilaria corticata) on different physiological parameters of TANUVAS Aseel chicken. A total of 30 TANUVAS Aseel chicks, day old were allocated into three groups (Each with 10 chicks) viz., one control group (C1) and two treatment groups (T1 and T2). The control group (C1) were fed with commercial grower feed alone and the treatment group (T1) had control diet with 5% Sargassum wightii and the treatment group (T2) had control diet mixed with 5% Gracilaria corticata. Parameters were recorded for a continuous period of 16 weeks in 4 weeks interval. The growth performance was found to be non-significant (P ≥ 0.05) during 0 day of feed trials and highly significant (P ≤ 0.01) during 4th, 8th, 12th and 16th week of feed trails. Haematological parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference exist in lymphocyte, monocyte and eosinophil count and a highly significant (P ≤ 0.01) difference exist in platelets count between the control (C1) and treatment groups (T1 and T2). Serum biochemical parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference in Uric acid, creatinine, ALT, calcium and cholesterol level and a highly significant (P ≤ 0.01) difference in Total protein, albumin, AST, glucose, triglycerides, phosphorus, magnesium, potassium and chloride level between the control (C1) and treatment groups (T1 and T2). However non-significant (P ≥ 0.05) difference exists in globulin and sodium content only during 16th week of feed trials. It could be noted that 5% inclusion of red seaweed, Gracilaria corticata in commercial grower feed as a feed supplement to TANUVAS Aseel chicks had a better body weight gain than 5% inclusion level of brown seaweed, Sargassum wightii in commercial grower feed. Based on the haematological and serum biochemical analysis, the supplementation of 5% inclusion level of seaweeds in this trial did not pose any threat to the physiological well-being of TANUVAS Aseel chicken.
{"title":"Effect of dietary supplementation of seaweeds on growth and blood profiles of TANUVAS Aseel chicken","authors":"TA Vijayalingam, NV Rajesh, S Vairamuthu, M Boopathy Raja, NK Sudeep Kumar","doi":"10.12681/jhvms.30812","DOIUrl":"https://doi.org/10.12681/jhvms.30812","url":null,"abstract":"The present study was conducted to evaluate the dietary effects of two seaweeds (Sargassum wightii and Gracilaria corticata) on different physiological parameters of TANUVAS Aseel chicken. A total of 30 TANUVAS Aseel chicks, day old were allocated into three groups (Each with 10 chicks) viz., one control group (C1) and two treatment groups (T1 and T2). The control group (C1) were fed with commercial grower feed alone and the treatment group (T1) had control diet with 5% Sargassum wightii and the treatment group (T2) had control diet mixed with 5% Gracilaria corticata. Parameters were recorded for a continuous period of 16 weeks in 4 weeks interval. The growth performance was found to be non-significant (P ≥ 0.05) during 0 day of feed trials and highly significant (P ≤ 0.01) during 4th, 8th, 12th and 16th week of feed trails. Haematological parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference exist in lymphocyte, monocyte and eosinophil count and a highly significant (P ≤ 0.01) difference exist in platelets count between the control (C1) and treatment groups (T1 and T2). Serum biochemical parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference in Uric acid, creatinine, ALT, calcium and cholesterol level and a highly significant (P ≤ 0.01) difference in Total protein, albumin, AST, glucose, triglycerides, phosphorus, magnesium, potassium and chloride level between the control (C1) and treatment groups (T1 and T2). However non-significant (P ≥ 0.05) difference exists in globulin and sodium content only during 16th week of feed trials. It could be noted that 5% inclusion of red seaweed, Gracilaria corticata in commercial grower feed as a feed supplement to TANUVAS Aseel chicks had a better body weight gain than 5% inclusion level of brown seaweed, Sargassum wightii in commercial grower feed. Based on the haematological and serum biochemical analysis, the supplementation of 5% inclusion level of seaweeds in this trial did not pose any threat to the physiological well-being of TANUVAS Aseel chicken.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Type 1 diabetes (T1D) is a chronic autoimmune disease that even with insulin therapy, inflammatory complications will develop in the long term. 40 inbred C57BL/6 mice were randomly divided into four groups (n=10): Control group consisted of healthy mice receiving citrate buffer, Diabetic group included a group of diabetic mice, Diabetic+TX group was a group of diabetic mice treated with troxerutin (TX), and TX group was a group of healthy mice treated with TX. Two weeks after the final dose of streptozotocin (STZ), The cytokine levels were measured using ELISA in the culture supernatants of spleen cells after 72 hours. Radioimmunoassay was used to measure insulin and c-peptide levels. The fasting blood sugar (FBS) was measured by an automatic glucometer device. lymphocyte proliferation index was evaluated using MTT assay, myeloperoxidase (MPO) level was measured in serum and pathologic studies of the kidney and liver were performed. The levels of IL-1, IL-17, TNF-α and IFN-γ as well as MPO, FBS levels and proliferation index was significantly decreased in the treated diabetic group compared to the diabetic mice (p<0.05). plasma C-peptide and insulin significantly increased in treated diabetic group than in the diabetic mice (p<0.05). Histologically, in diabetic animals treated with Tx, inflammatory and degenerative processes in both kidney and liver tissues were alleviated significantly (p<0.05). According to the results, it was supported the anti-diabetic and anti-inflammatory effects of TX, however, more studies are needed to investigate the effects of TX and the dose-response relationship in this disease.
{"title":"Troxerutin as an anti-inflammatory and anti-oxidative drug could ameliorate Type 1 diabetes complications in C57BL/6 mice","authors":"Y Jafari-Khataylou, S Ahmadiafshar, N Mirzakhani","doi":"10.12681/jhvms.31426","DOIUrl":"https://doi.org/10.12681/jhvms.31426","url":null,"abstract":"Type 1 diabetes (T1D) is a chronic autoimmune disease that even with insulin therapy, inflammatory complications will develop in the long term. 40 inbred C57BL/6 mice were randomly divided into four groups (n=10): Control group consisted of healthy mice receiving citrate buffer, Diabetic group included a group of diabetic mice, Diabetic+TX group was a group of diabetic mice treated with troxerutin (TX), and TX group was a group of healthy mice treated with TX. Two weeks after the final dose of streptozotocin (STZ), The cytokine levels were measured using ELISA in the culture supernatants of spleen cells after 72 hours. Radioimmunoassay was used to measure insulin and c-peptide levels. The fasting blood sugar (FBS) was measured by an automatic glucometer device. lymphocyte proliferation index was evaluated using MTT assay, myeloperoxidase (MPO) level was measured in serum and pathologic studies of the kidney and liver were performed. The levels of IL-1, IL-17, TNF-α and IFN-γ as well as MPO, FBS levels and proliferation index was significantly decreased in the treated diabetic group compared to the diabetic mice (p<0.05). plasma C-peptide and insulin significantly increased in treated diabetic group than in the diabetic mice (p<0.05). Histologically, in diabetic animals treated with Tx, inflammatory and degenerative processes in both kidney and liver tissues were alleviated significantly (p<0.05). According to the results, it was supported the anti-diabetic and anti-inflammatory effects of TX, however, more studies are needed to investigate the effects of TX and the dose-response relationship in this disease.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AR Belmamoun, A Ammam, C Mhamdia, R Chadli, A Baki, N Madouni
Raw milk contamination by Methicillin-resistant S. aureus (MRSA) threatens food safety and leads to public health problems. Our study evaluates biofilm formation and virulence factors among 21 MRSA in raw cow’s milk. Methicillin resistance was confirmed by cefoxitin screening using the automated VITEK2 system, with a minimum inhibitory concentration greater than 8 mg/l. Qualitative characterization of biofilm-producing strains was performed on Congo red agar (CRA), with a semi-quantitative adhesion test on 96-well tissue culture plates (TCP). The ability to produce different enzymes was evaluated, such as caseinase, lipase, and phospholipase (lecithinase). The surface hydrophobicity of the bacteria was determined, and the autoaggregation test was used to predict the interactions between bacterial cells. Among the tested strains, 61.9% were biofilm producers in the CRA, developing a positive and variable phenotype. Furthermore, 19.05% and 80.95% of isolates were high and low biofilm formation on TCP. The enzymatic activity showed that lécithinase, caseinase, and lipase activities were detected in 100%, 80.95%, and 80.95% of cases. Highly hydrophilic (85.71%) and weakly hydrophobic (14.29%) were detected in MRSA isolates. The strains showed that 71.43% were moderate autoaggregation and 28.57% were low autoaggregation. No significant difference was found between the CRA method and TCP (P> 0.05). A significant association was found between adhesion capacity and bacterial autoaggregation in S. aureus strains (p>0.05). On the other hand, no statistical association between the hydrophobicity of microbial strains and adhesion capacity (p<0.05) was found. The same result was for the hydrophobicity of microbial strains and autoaggregation (p<0.05). This investigation could be beneficial for developing new control measures, prevention, and effective treatment against staphylococcal bacterial resistance.
{"title":"Presence of methicillin-resistant Staphylococcus aureus in raw cow’s milk: adhesive capacities and extracellular enzymes characterization","authors":"AR Belmamoun, A Ammam, C Mhamdia, R Chadli, A Baki, N Madouni","doi":"10.12681/jhvms.31440","DOIUrl":"https://doi.org/10.12681/jhvms.31440","url":null,"abstract":"Raw milk contamination by Methicillin-resistant S. aureus (MRSA) threatens food safety and leads to public health problems. Our study evaluates biofilm formation and virulence factors among 21 MRSA in raw cow’s milk. Methicillin resistance was confirmed by cefoxitin screening using the automated VITEK2 system, with a minimum inhibitory concentration greater than 8 mg/l. Qualitative characterization of biofilm-producing strains was performed on Congo red agar (CRA), with a semi-quantitative adhesion test on 96-well tissue culture plates (TCP). The ability to produce different enzymes was evaluated, such as caseinase, lipase, and phospholipase (lecithinase). The surface hydrophobicity of the bacteria was determined, and the autoaggregation test was used to predict the interactions between bacterial cells. Among the tested strains, 61.9% were biofilm producers in the CRA, developing a positive and variable phenotype. Furthermore, 19.05% and 80.95% of isolates were high and low biofilm formation on TCP. The enzymatic activity showed that lécithinase, caseinase, and lipase activities were detected in 100%, 80.95%, and 80.95% of cases. Highly hydrophilic (85.71%) and weakly hydrophobic (14.29%) were detected in MRSA isolates. The strains showed that 71.43% were moderate autoaggregation and 28.57% were low autoaggregation. No significant difference was found between the CRA method and TCP (P> 0.05). A significant association was found between adhesion capacity and bacterial autoaggregation in S. aureus strains (p>0.05). On the other hand, no statistical association between the hydrophobicity of microbial strains and adhesion capacity (p<0.05) was found. The same result was for the hydrophobicity of microbial strains and autoaggregation (p<0.05). This investigation could be beneficial for developing new control measures, prevention, and effective treatment against staphylococcal bacterial resistance.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Voulgarakis, D Gougoulis, D Psalla, G Papakonstantinou, M Angelidou-Tsifida, V Papatsiros, LV Athanasiou, G Christodoulopoulos
Abstract
Ruminal acidosis is presented as the most considerable nutritional disorder of ruminants with severe impacts in animal health, animal welfare and considerable economics losses in ovine and bovine herds. The disease can be distinguished as Acute (ARA) and Sub-Acute Ruminal Acidosis (SARA). SARA constitutes as the main nutritional disorder in intensive ruminant farming with several complications, such as liver abscesses, milk fat depression, reduced milk yield and early culling. This paper constitutes the first part of a thorough review about ruminal acidosis. The first part focuses on definition, types of ruminal acidosis, epidemiology, economic impacts and welfare implications.
{"title":"Ruminal Acidosis Part I: Clinical manifestations, epidemiology and impact of the disease","authors":"N Voulgarakis, D Gougoulis, D Psalla, G Papakonstantinou, M Angelidou-Tsifida, V Papatsiros, LV Athanasiou, G Christodoulopoulos","doi":"10.12681/jhvms.31237","DOIUrl":"https://doi.org/10.12681/jhvms.31237","url":null,"abstract":"Abstract
 Ruminal acidosis is presented as the most considerable nutritional disorder of ruminants with severe impacts in animal health, animal welfare and considerable economics losses in ovine and bovine herds. The disease can be distinguished as Acute (ARA) and Sub-Acute Ruminal Acidosis (SARA). SARA constitutes as the main nutritional disorder in intensive ruminant farming with several complications, such as liver abscesses, milk fat depression, reduced milk yield and early culling. This paper constitutes the first part of a thorough review about ruminal acidosis. The first part focuses on definition, types of ruminal acidosis, epidemiology, economic impacts and welfare implications.
","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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