Fish eosinophilic granular cells are found in the connective tissues of structures associated with the external environment. These cells are similar to mammalian mast cells in terms of structural and functional characteristics. The cytoplasmic granules of these cells give different staining reactions depending on fixative type. This study aimed to determine the staining properties and densities of eosinophilic granular cells in Oscar fish (Astronotus ocellatus Agassiz, 1831) intestine using different fixatives and histochemical techniques. Formalin and basic lead acetate fixation-giemsa staining indicated that eosinophilic granular cells were abundant in anterior intestine, localizing around especially the blood vessels and submucosa. Giemsa staining of Bouin’s fixed rather than other fixatives showed that eosinophilic granular cells were higher in posterior intestine. No reaction was observed in eosinophilic granular cells in Thionin and Toluidine Blue staining in any fixative. Eosinophilic granular cells in samples fixed with different fixatives had metachromatic Alcian Blue staining. In conclusion, this study shows that fixatives may have different effects on the distribution and staining properties of eosinophilic granular cells in Oscar fish intestinal regions. Abundance in anterior intestine and around submucosal blood vessels shows that eosinophilic granular cells could play an active role in mucosal immunity against food-borne pathogens.
{"title":"Density, Distribution and Staining Properties of Eosinophilic Granular Cells in Oscar Fish (Astronotus ocellatus) Intestine","authors":"M. Öztop","doi":"10.12681/jhvms.28621","DOIUrl":"https://doi.org/10.12681/jhvms.28621","url":null,"abstract":"Fish eosinophilic granular cells are found in the connective tissues of structures associated with the external environment. These cells are similar to mammalian mast cells in terms of structural and functional characteristics. The cytoplasmic granules of these cells give different staining reactions depending on fixative type. This study aimed to determine the staining properties and densities of eosinophilic granular cells in Oscar fish (Astronotus ocellatus Agassiz, 1831) intestine using different fixatives and histochemical techniques. Formalin and basic lead acetate fixation-giemsa staining indicated that eosinophilic granular cells were abundant in anterior intestine, localizing around especially the blood vessels and submucosa. Giemsa staining of Bouin’s fixed rather than other fixatives showed that eosinophilic granular cells were higher in posterior intestine. No reaction was observed in eosinophilic granular cells in Thionin and Toluidine Blue staining in any fixative. Eosinophilic granular cells in samples fixed with different fixatives had metachromatic Alcian Blue staining. In conclusion, this study shows that fixatives may have different effects on the distribution and staining properties of eosinophilic granular cells in Oscar fish intestinal regions. Abundance in anterior intestine and around submucosal blood vessels shows that eosinophilic granular cells could play an active role in mucosal immunity against food-borne pathogens.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49292389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the effect of dietary supplementation of oregano and rosemary essential oils (EO) on growth performance and cecal microbiota of broilers were investigated. A total of 450 1-d-old male Ross-308 broilers were divided into 5-experimental groups (10 replicates of 9 chickens): a Control (C), fed a basal diet; four treatments, which received a basal diet supplemented with oregano and rosemary EOs individually (O, 300 mg/kg oregano EO; R, 300 mg/kg rosemary EO) and combined (OR1, 150 mg/kg oregano EO + 150 mg/kg rosemary EO; OR2, 200 mg/kg oregano EO + 200 mg/kg rosemary EO). Body weight (BW), feed intake (FI), body weight gain (BWG), feed conversion ratio (FCR), and cecal microbiota (coliforms, clostridia and lactobacilli) were determined weekly, and at 42 d, respectively. BW in R (p<0.05) and OR2 (p<0.001), and BWG and FCR in OR2 (p<0.05) were significantly higher than C at 42 d, despite no difference in FI in any group during experimental period. Counts of cecal coliforms (p<0.001) and clostridia (p<0.01) decreased, and lactobacilli (p<0.001) increased substantially between C and treatment groups. Results indicated that combined oregano and rosemary EO (200 mg/kg ea) supplementation significantly increased BW and BWG, improved FCR in 1-42 d, lowered coliform and clostridial, and increased lactobacilli counts suggesting a beneficial shift in cecal microbiota.
{"title":"Effect of Dietary Oregano and Rosemary Essential Oil Supplementation on Growth Performance and Cecal Microbiota of Broilers","authors":"E. Cetin, B. Anar, S. Temelli, SS Cengiz, M. Eren","doi":"10.12681/jhvms.28626","DOIUrl":"https://doi.org/10.12681/jhvms.28626","url":null,"abstract":"In this study, the effect of dietary supplementation of oregano and rosemary essential oils (EO) on growth performance and cecal microbiota of broilers were investigated. A total of 450 1-d-old male Ross-308 broilers were divided into 5-experimental groups (10 replicates of 9 chickens): a Control (C), fed a basal diet; four treatments, which received a basal diet supplemented with oregano and rosemary EOs individually (O, 300 mg/kg oregano EO; R, 300 mg/kg rosemary EO) and combined (OR1, 150 mg/kg oregano EO + 150 mg/kg rosemary EO; OR2, 200 mg/kg oregano EO + 200 mg/kg rosemary EO). Body weight (BW), feed intake (FI), body weight gain (BWG), feed conversion ratio (FCR), and cecal microbiota (coliforms, clostridia and lactobacilli) were determined weekly, and at 42 d, respectively. BW in R (p<0.05) and OR2 (p<0.001), and BWG and FCR in OR2 (p<0.05) were significantly higher than C at 42 d, despite no difference in FI in any group during experimental period. Counts of cecal coliforms (p<0.001) and clostridia (p<0.01) decreased, and lactobacilli (p<0.001) increased substantially between C and treatment groups. Results indicated that combined oregano and rosemary EO (200 mg/kg ea) supplementation significantly increased BW and BWG, improved FCR in 1-42 d, lowered coliform and clostridial, and increased lactobacilli counts suggesting a beneficial shift in cecal microbiota.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44550667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. KALAYCI YÜKSEK, D. Gümüş, AC Macunluoğlu, E. Eroğlu, D. Camadan, M. Anğ Küçüker
Abstract Multidrug resistance is a great challenge for the treatment of infectious diseases. We determined antibiotic resistance patterns, integrons, plasmid-mediated ESBL-, AmpC beta-lactamase-, carbapenemase-, colistin resistance genes, plasmid replicon types and phylogeny of fecal E. coli strains isolated from domestic cats and dogs in Turkey. A total of 104 fecal samples of healthy 49 cats and 55 dogs were examined. The integrons, plasmid-mediated resistance genes, plasmid replicon types and phylogroups were determined by PCR. Antimicrobial susceptibilities were performed by disc diffusion and microdilution methods. coli strains were mostly resistant to AMP (56.73%), SXT (39.42%), CTX (38.46%) and CIP (30.77%). Colistin resistance was not detected. ESBL and carbapenemase rates were 35.5 % and 7.69%, respectively. Eighty (76.9%) and 49 (47.1%) strains were harboring class I and class II integrons, respectively. Besides 12 strains were shown to possess class III integrons. The most frequently detected genes were blaCTX-M (48.08%), blaTEM (45.19%) and blaVIM (20.19%). In our study, none of strains were positive for mcr-1 and mcr-2 genes. Integrons were mostly found on plasmids of incompatibility groups IncF (71.25%) and strains bearing CTX-M and TEM carried a wide range of plasmid replicons of which IncF, IncFIB, IncK, and IncN. The majority of the strains were grouped in B2 (31.73%) and B1 (22.12%) and resistant bacteria mostly belonged to phylogroup B2. We showed an increasing trend in ESBL-producing E. coli among fecal microbiota members. E. coli strains with different plasmid replicon types and phylogroups isolated from cats and dogs can be resistant to various antibiotics which are used in human and veterinary medicine.
{"title":"Mobile resistance determinants, plasmid replicon types and phylogeny among Escherichia coli strains isolated from cats and dogs","authors":"F. KALAYCI YÜKSEK, D. Gümüş, AC Macunluoğlu, E. Eroğlu, D. Camadan, M. Anğ Küçüker","doi":"10.12681/jhvms.30148","DOIUrl":"https://doi.org/10.12681/jhvms.30148","url":null,"abstract":"Abstract \u0000Multidrug resistance is a great challenge for the treatment of infectious diseases. We determined antibiotic resistance patterns, integrons, plasmid-mediated ESBL-, AmpC beta-lactamase-, carbapenemase-, colistin resistance genes, plasmid replicon types and phylogeny of fecal E. coli strains isolated from domestic cats and dogs in Turkey. \u0000A total of 104 fecal samples of healthy 49 cats and 55 dogs were examined. The integrons, plasmid-mediated resistance genes, plasmid replicon types and phylogroups were determined by PCR. Antimicrobial susceptibilities were performed by disc diffusion and microdilution methods. \u0000 \u0000 coli strains were mostly resistant to AMP (56.73%), SXT (39.42%), CTX (38.46%) and CIP (30.77%). Colistin resistance was not detected. ESBL and carbapenemase rates were 35.5 % and 7.69%, respectively. Eighty (76.9%) and 49 (47.1%) strains were harboring class I and class II integrons, respectively. Besides 12 strains were shown to possess class III integrons. The most frequently detected genes were blaCTX-M (48.08%), blaTEM (45.19%) and blaVIM (20.19%). In our study, none of strains were positive for mcr-1 and mcr-2 genes. Integrons were mostly found on plasmids of incompatibility groups IncF (71.25%) and strains bearing CTX-M and TEM carried a wide range of plasmid replicons of which IncF, IncFIB, IncK, and IncN. The majority of the strains were grouped in B2 (31.73%) and B1 (22.12%) and resistant bacteria mostly belonged to phylogroup B2. \u0000 \u0000We showed an increasing trend in ESBL-producing E. coli among fecal microbiota members. E. coli strains with different plasmid replicon types and phylogroups isolated from cats and dogs can be resistant to various antibiotics which are used in human and veterinary medicine.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47492943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phenotypic and genotypic antimicrobial resistances of Campylobacter strains from chicken meat were investigated. A total of 19 isolates (Campylobacter jejuni=6 and Campylobacter coli=13) were identified by multiplex PCR. The C. jejuni and C. coli isolates were tested for resistance to ciprofloxacin, erythromycin, and tetracycline using the disc diffusion method. In addition, all the strains were analyzed for resistance gene tet-O and mutations in the gyrA and 23S rRNA genes by PCR. Overall, a high frequency of resistance was detected against tetracycline (14/19, 73.6%), followed by erythromycin (7/19, 36.8%), and ciprofloxacin (6/19, 31.5%). Two of C. coli isolates (15.3%) were multidrug resistant, whereas none of the C. jejuni isolates were resistant to three antibiotics at the same time. Ten of the 14 Campylobacter strains (71.4%) resistant to tetracycline carried tet-O gene. Mutations in the 23S rRNA and gyrA genes were identified in 57.1% (4/7), 66.6% (4/6) of the isolates resistant to erythromycin and ciprofloxacin, respectively.
{"title":"Phenotypic and genotypic characterization of antimicrobial resistance of Campylobacterisolates from poultry meat in Turkey","authors":"Hyung-Kee Can","doi":"10.12681/jhvms.28709","DOIUrl":"https://doi.org/10.12681/jhvms.28709","url":null,"abstract":"Phenotypic and genotypic antimicrobial resistances of Campylobacter strains from chicken meat were investigated. A total of 19 isolates (Campylobacter jejuni=6 and Campylobacter coli=13) were identified by multiplex PCR. The C. jejuni and C. coli isolates were tested for resistance to ciprofloxacin, erythromycin, and tetracycline using the disc diffusion method. In addition, all the strains were analyzed for resistance gene tet-O and mutations in the gyrA and 23S rRNA genes by PCR. Overall, a high frequency of resistance was detected against tetracycline (14/19, 73.6%), followed by erythromycin (7/19, 36.8%), and ciprofloxacin (6/19, 31.5%). Two of C. coli isolates (15.3%) were multidrug resistant, whereas none of the C. jejuni isolates were resistant to three antibiotics at the same time. Ten of the 14 Campylobacter strains (71.4%) resistant to tetracycline carried tet-O gene. Mutations in the 23S rRNA and gyrA genes were identified in 57.1% (4/7), 66.6% (4/6) of the isolates resistant to erythromycin and ciprofloxacin, respectively. ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47516312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Azeem, H. Akbar, L. Ahmad, M. Ashraf, S. Puvanendiran
Finding alternate ways to relieve animals from the ectoparasites is one of the most important aspects under One-Health concept. Herbal ectoparasiticides offer a promising alternate to chemical ectoparasiticides. The present study was designed to evaluate the ectoparasiticide efficacy of Nimboliâ (Neem: Azadirachta indica extract) in various animal species, keeping in view the objectives of One-Health. The ectoparasiticide efficacy of Nimboliâ was also compared with a commercial chemical ectoparasiticide Tagafon (Hydroxyethyl Phosporic Acid). Various animals infested with ticks, fleas, lice, flies, and maggots and presented at clinics were treated with Nimboliâ(n=36)and Tagafon (n=19). Mean time taken by each ectoparasiticide to knock-down various ectoparasites was recorded. The present study suggested significantly higher efficacy of Nimboliâ against ticks, fleas, lice, flies and maggots as compared with Tagafon. The mean time taken by Nimboli® to knockdown various ectoparasites of same and different kinds and their larvae was also less compared to the mean time taken by Tagafon. The present study indicated that Nimboliâ is an eco-friendly ectoparasiticide that is safe-to-use both for animals and their handlers, hence can replace chemical ectoparasiticides. The present study highlights the importance of herbal ectoparasiticides, especially those containing neem in the treatment and control of ectoparasites in animals. This information have special relevance for countries that have native neem trees such as Sri Lanka, Pakistan, India, Indonesia, Malaysia, Myanmar, Senegal, and Thailand. Since herbal ectoparasiticides are biodegradable, they offer an added advantage of maintaining ecological health.
{"title":"When used in animals, a neem (Azadirachta indica)-based ectoparasiticide performs better than a synthetic one","authors":"S. Azeem, H. Akbar, L. Ahmad, M. Ashraf, S. Puvanendiran","doi":"10.12681/jhvms.27963","DOIUrl":"https://doi.org/10.12681/jhvms.27963","url":null,"abstract":"Finding alternate ways to relieve animals from the ectoparasites is one of the most important aspects under One-Health concept. Herbal ectoparasiticides offer a promising alternate to chemical ectoparasiticides. The present study was designed to evaluate the ectoparasiticide efficacy of Nimboliâ (Neem: Azadirachta indica extract) in various animal species, keeping in view the objectives of One-Health. The ectoparasiticide efficacy of Nimboliâ was also compared with a commercial chemical ectoparasiticide Tagafon (Hydroxyethyl Phosporic Acid). Various animals infested with ticks, fleas, lice, flies, and maggots and presented at clinics were treated with Nimboliâ(n=36)and Tagafon (n=19). Mean time taken by each ectoparasiticide to knock-down various ectoparasites was recorded. The present study suggested significantly higher efficacy of Nimboliâ against ticks, fleas, lice, flies and maggots as compared with Tagafon. The mean time taken by Nimboli® to knockdown various ectoparasites of same and different kinds and their larvae was also less compared to the mean time taken by Tagafon. The present study indicated that Nimboliâ is an eco-friendly ectoparasiticide that is safe-to-use both for animals and their handlers, hence can replace chemical ectoparasiticides. The present study highlights the importance of herbal ectoparasiticides, especially those containing neem in the treatment and control of ectoparasites in animals. This information have special relevance for countries that have native neem trees such as Sri Lanka, Pakistan, India, Indonesia, Malaysia, Myanmar, Senegal, and Thailand. Since herbal ectoparasiticides are biodegradable, they offer an added advantage of maintaining ecological health.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43543583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DY Yeşilbağ, İ. Çeti̇n, SS Cengiz, E. Cetin, M. Sarpasar
The aim of this study was to evaluate the effects of β-vinasse supplementation on the live weight (LW), live weight gain (LWG), feed intake (FI), feed conversion ratio (FCR), carcass yield, meat quality and ilio-caecal bacteriological flora of quails. A total of 240 5-d-old Japanese (Coturnix coturnix Japonica) quails, including both males and females, were divided into 3 groups 80 quails and treated as follow: a control group with 0 g β-vinasse/kg ration; (2) 15 g/kg β-vinasse and (3) 30 g/kg β-vinasse. The study lasted for 6 weeks. At the end of the experiment, suplementation with β-vinasse did not have significant effect on FI and FCR. Dietary supplementation with 30 g/kg β-vinasse significantly (P < 0.05) increased LW (21 d) and LWG (5 to 21 d). The dietary treatment of quails with different levels of β- vinasse did not effect on hot carcass weight, cold carcass weights, hot and cold carcass yields and breast and thigh pH. The lightness (L*) and yellowness (b*) of breast values were significantly (P<0.05) increased of 30 g/kg supplementation. Different levels of β-vinasse significantly increased Lactobacillus spp. in faeces. As a result, it was concluded that β-vinasse (by-product obtained from molasses) can be used in quail diets as an alternative feed source that will meet the nutritional needs of the animal and have positive effects on the digestive system, especially on the intestinal health ( an increase in Lactobacillus spp. counts).
{"title":"Effects of Beta Vinasse Supplementation on Performance, Meat Quality and Ilio- Caecal Microflora in Quail Rations","authors":"DY Yeşilbağ, İ. Çeti̇n, SS Cengiz, E. Cetin, M. Sarpasar","doi":"10.12681/jhvms.28016","DOIUrl":"https://doi.org/10.12681/jhvms.28016","url":null,"abstract":"The aim of this study was to evaluate the effects of β-vinasse supplementation on the live weight (LW), live weight gain (LWG), feed intake (FI), feed conversion ratio (FCR), carcass yield, meat quality and ilio-caecal bacteriological flora of quails. A total of 240 5-d-old Japanese (Coturnix coturnix Japonica) quails, including both males and females, were divided into 3 groups 80 quails and treated as follow: a control group with 0 g β-vinasse/kg ration; (2) 15 g/kg β-vinasse and (3) 30 g/kg β-vinasse. The study lasted for 6 weeks. At the end of the experiment, suplementation with β-vinasse did not have significant effect on FI and FCR. Dietary supplementation with 30 g/kg β-vinasse significantly (P < 0.05) increased LW (21 d) and LWG (5 to 21 d). The dietary treatment of quails with different levels of β- vinasse did not effect on hot carcass weight, cold carcass weights, hot and cold carcass yields and breast and thigh pH. The lightness (L*) and yellowness (b*) of breast values were significantly (P<0.05) increased of 30 g/kg supplementation. Different levels of β-vinasse significantly increased Lactobacillus spp. in faeces. As a result, it was concluded that β-vinasse (by-product obtained from molasses) can be used in quail diets as an alternative feed source that will meet the nutritional needs of the animal and have positive effects on the digestive system, especially on the intestinal health ( an increase in Lactobacillus spp. counts).","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46379182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current research was carried out to investigate the effect of diets enriched with different levels of organic iron on performance, egg quality, blood haematological parameters, and serum biochemical constituents in laying quails. In the study, 120 female quails at 20 weeks of age were allocated to 5 treatment groups with 6 replicates of 4 quails in each. Experimental diets were formed by adding 0, 50, 100, 150, and 200 mg/kg of iron (iron-glycine) to the diet containing 96 mg/kg of iron. Performance parameters of quails were not affected by the supplementation of organic iron to diet (P>0.05). The supplementation of organic iron affected as quadratic only the eggshell breaking strength among the egg external quality parameters, and it was maximum at 150 mg/kg organic iron addition (P<0.01). Egg yolk a* value, which is one of the egg internal quality parameters, was linearly affected by organic iron addition, but other parameters were not affected (P<0.01). The administration of organic iron to the diet linearly increased the serum iron level (P<0.01), the serum phosphorus level was affected quadratically (P<0.05) and reached a maximum at 150 mg/kg. Among the blood haematology parameters, white blood cell and monocyte counts linearly increased with the addition of organic iron to the diet (P<0.01), but other parameters were not affected. According to the results obtained from current research, it can be said that enrichment of laying quail diets with organic iron up to 150 mg/kg level improved eggshell breaking strength without affecting performance, however, it negatively affected blood parameters by increasing white blood cell and monocyte without changing blood red cell parameters.
{"title":"Influence of Organic Iron Enriched Diets on Performance, Egg Quality, Blood Haematological and Biochemical Constituents in Quails","authors":"O. Olgun, E. Gül, AÖ Yildiz","doi":"10.12681/jhvms.28232","DOIUrl":"https://doi.org/10.12681/jhvms.28232","url":null,"abstract":"The current research was carried out to investigate the effect of diets enriched with different levels of organic iron on performance, egg quality, blood haematological parameters, and serum biochemical constituents in laying quails. In the study, 120 female quails at 20 weeks of age were allocated to 5 treatment groups with 6 replicates of 4 quails in each. Experimental diets were formed by adding 0, 50, 100, 150, and 200 mg/kg of iron (iron-glycine) to the diet containing 96 mg/kg of iron. Performance parameters of quails were not affected by the supplementation of organic iron to diet (P>0.05). The supplementation of organic iron affected as quadratic only the eggshell breaking strength among the egg external quality parameters, and it was maximum at 150 mg/kg organic iron addition (P<0.01). Egg yolk a* value, which is one of the egg internal quality parameters, was linearly affected by organic iron addition, but other parameters were not affected (P<0.01). The administration of organic iron to the diet linearly increased the serum iron level (P<0.01), the serum phosphorus level was affected quadratically (P<0.05) and reached a maximum at 150 mg/kg. Among the blood haematology parameters, white blood cell and monocyte counts linearly increased with the addition of organic iron to the diet (P<0.01), but other parameters were not affected. According to the results obtained from current research, it can be said that enrichment of laying quail diets with organic iron up to 150 mg/kg level improved eggshell breaking strength without affecting performance, however, it negatively affected blood parameters by increasing white blood cell and monocyte without changing blood red cell parameters.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49548291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Schina, V. Dotas, D. Gourdouvelis, I. Kaimakamis, I. Mitsopoulos, G. Tsakanikas, V. Bampidis
Lean Meat Percentage (LMP) is a key index for assessing pork carcass quality as well as determining carcass classification and price. This framework offers to farmers the incentive to achieve the desired organoleptic features; therefore LMP must be measured objectively online, by means of various measuring instruments. The aim of this work was to assess two different methods for predicting the lean meat percentage of pig carcasses in Greece in comparison to the European reference method. For this reason, the same 130 pig carcasses (65 males and 65 females) were measured using 2 prediction devices (Optiscan-TP and Optigrade-MCP) and subsequently were assessed according to European reference method. A formula corresponding to each device was constructed using the ordinary least squares (OLS)method and excluding the same outliers. Calculated RMSEP for all 130 carcasses was 2.46518 for Optiscan-TP and 2.48489 for Optigrade-MCP. It can be concluded that both methods perform entirely according to EU legislation (Commission Regulation (EC) 1249/2008, European Union, 2008), and results obtained are similar regardless of different measurement points and technologies.
{"title":"A comparative assessment of two different methods, using devices Optiscan-TP and Optigrade-MCP, for predicting the lean meat percentage of pig carcasses in Greece in relation to the European reference method","authors":"V. Schina, V. Dotas, D. Gourdouvelis, I. Kaimakamis, I. Mitsopoulos, G. Tsakanikas, V. Bampidis","doi":"10.12681/jhvms.28409","DOIUrl":"https://doi.org/10.12681/jhvms.28409","url":null,"abstract":"Lean Meat Percentage (LMP) is a key index for assessing pork carcass quality as well as determining carcass classification and price. This framework offers to farmers the incentive to achieve the desired organoleptic features; therefore LMP must be measured objectively online, by means of various measuring instruments. The aim of this work was to assess two different methods for predicting the lean meat percentage of pig carcasses in Greece in comparison to the European reference method. For this reason, the same 130 pig carcasses (65 males and 65 females) were measured using 2 prediction devices (Optiscan-TP and Optigrade-MCP) and subsequently were assessed according to European reference method. A formula corresponding to each device was constructed using the ordinary least squares (OLS)method and excluding the same outliers. Calculated RMSEP for all 130 carcasses was 2.46518 for Optiscan-TP and 2.48489 for Optigrade-MCP. It can be concluded that both methods perform entirely according to EU legislation (Commission Regulation (EC) 1249/2008, European Union, 2008), and results obtained are similar regardless of different measurement points and technologies.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48275663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Adami Ghamsari, M. Tajabadi Ebrahimi, M. Bagheri Varzaneh, A. Iranbakhsh, A. Akhavan Sepahi
Mycotoxins contamination occurring in dairy cow's diet is responsible for devastating effects on livestock health. Among different strategies, using organic adsorbents is a promising approach to reduce the toxicity of mycotoxins. This study investigated the effects of an organic adsorbent containing Lactobacillus brevis TD4, Lactobacillus paracasei TD3, and Saccharomyces cerevisiae cell wall on milk production, somatic cell count, blood parameters (white blood cell [WBC], lymphocyte [LYM], neutrophil, basophil, monocyte, eosinophil, red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin) and liver function (alanine transaminase [ALT], gamma-glutamyl transferase [GGT], aspartate transaminase [AST], alkaline phosphatase [ALP], urea, total protein, albumin) in dairy cows fed a naturally contaminated diet with Zearalenone and Deoxynivalenol. The amounts of mycotoxins Zearalenone (ZEA) and Deoxynivalenol (DON) in feed ingredients were measured using the HPLC method. Ten Holstein dairy cows received organic adsorbents daily in their diets for four weeks. Milk and blood samples were taken from cows before the start of feeding adsorbent (CTRL), during the feeding period (FP), and one week after removal of adsorbent from the diet (RP). Totally, the amount of measured ZEA and DON toxins in the diet were 389 and 1254.6 ppb, respectively. Feeding of organic adsorbent significantly increased milk production, total serum protein, and albumin compared to CTRL (P<0.01). Also, numerically lower somatic cell count in their milk and a significant decrease (P<0.01) in serum urea were resulted. Among examined blood parameters, the number of WBC and LYM significantly decreased (P<0.01) after feeding with the organic binder in comparison to the control period. Furthermore, except for a significant increase in the level of AST (P<0.05), the other liver function examined parameters were not affected. The consumption of feed containing low-cost organic adsorbent including Lactobacillus bacteria and yeast cell wall can improve the physical condition and health of dairy cows and reduce economic losses in livestock production.
{"title":"Effect of Adding an Organic Binder on Health of Cows Fed with Mycotoxins Contaminated Diet","authors":"F. Adami Ghamsari, M. Tajabadi Ebrahimi, M. Bagheri Varzaneh, A. Iranbakhsh, A. Akhavan Sepahi","doi":"10.12681/jhvms.28266","DOIUrl":"https://doi.org/10.12681/jhvms.28266","url":null,"abstract":"Mycotoxins contamination occurring in dairy cow's diet is responsible for devastating effects on livestock health. Among different strategies, using organic adsorbents is a promising approach to reduce the toxicity of mycotoxins. This study investigated the effects of an organic adsorbent containing Lactobacillus brevis TD4, Lactobacillus paracasei TD3, and Saccharomyces cerevisiae cell wall on milk production, somatic cell count, blood parameters (white blood cell [WBC], lymphocyte [LYM], neutrophil, basophil, monocyte, eosinophil, red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin) and liver function (alanine transaminase [ALT], gamma-glutamyl transferase [GGT], aspartate transaminase [AST], alkaline phosphatase [ALP], urea, total protein, albumin) in dairy cows fed a naturally contaminated diet with Zearalenone and Deoxynivalenol. The amounts of mycotoxins Zearalenone (ZEA) and Deoxynivalenol (DON) in feed ingredients were measured using the HPLC method. Ten Holstein dairy cows received organic adsorbents daily in their diets for four weeks. Milk and blood samples were taken from cows before the start of feeding adsorbent (CTRL), during the feeding period (FP), and one week after removal of adsorbent from the diet (RP). Totally, the amount of measured ZEA and DON toxins in the diet were 389 and 1254.6 ppb, respectively. Feeding of organic adsorbent significantly increased milk production, total serum protein, and albumin compared to CTRL (P<0.01). Also, numerically lower somatic cell count in their milk and a significant decrease (P<0.01) in serum urea were resulted. Among examined blood parameters, the number of WBC and LYM significantly decreased (P<0.01) after feeding with the organic binder in comparison to the control period. Furthermore, except for a significant increase in the level of AST (P<0.05), the other liver function examined parameters were not affected. The consumption of feed containing low-cost organic adsorbent including Lactobacillus bacteria and yeast cell wall can improve the physical condition and health of dairy cows and reduce economic losses in livestock production.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48776658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
KM Malik, I. Zahoor, A. Khalique, AW Sahota, A. Basheer
Aseel and Naked neck are major chicken breeds of the tropics and well-known for their thermotolerance and robustness. However, both of them especially Aseel are very susceptible to Avian Influenza (AI) which cause huge mortalities. The role of cytokines in relation to the pathology and severity of the disease caused by the endemic strain (H9N2) of AIV in these breeds remained thus far unclear. The aim of this study was to investigate the effects of H9N2 AIV on the expression level of IL-1β, IL-6 and IL-8 in the lung tissues of Aseel, crossbred Naked Neck, and White Leghorn (WLH). In total 60 birds, 20 from each breed, were used in this study, whereas 30 birds (10 from each breed) were challenged intranasally with H9N2 virus with a concentration of 106 EID50 at 6wk of age and other half were treated as control. The lung tissues were sampled at 5th day post-infection to study the differential expression of IL-1β, IL-6, and IL-8 using qRT-PCR. Our data revealed a significant difference (P<0.001) in the gene expression levels among all the breeds in response to viral challenge. It was also observed that after exposure to H9N2 virus, Aseel birds showed the highest increase in their expressions of interleukin (IL-1 β, IL-6, and IL-8) genes followed by Naked Neck and WLH respectively suggesting greater susceptibility of Aseel to AIV compared with other breeds. However, these results are in agreement with the severity of disease and incidence of mortality caused by AI in these breeds.
{"title":"Expression profiling of IL-1β, IL-6 and IL-8 genes in lung tissues of Aseel, Crossbred Naked neck, and White Leghorn chicken challenged with H9N2 Influenza virus","authors":"KM Malik, I. Zahoor, A. Khalique, AW Sahota, A. Basheer","doi":"10.12681/jhvms.28174","DOIUrl":"https://doi.org/10.12681/jhvms.28174","url":null,"abstract":"Aseel and Naked neck are major chicken breeds of the tropics and well-known for their thermotolerance and robustness. However, both of them especially Aseel are very susceptible to Avian Influenza (AI) which cause huge mortalities. The role of cytokines in relation to the pathology and severity of the disease caused by the endemic strain (H9N2) of AIV in these breeds remained thus far unclear. The aim of this study was to investigate the effects of H9N2 AIV on the expression level of IL-1β, IL-6 and IL-8 in the lung tissues of Aseel, crossbred Naked Neck, and White Leghorn (WLH). In total 60 birds, 20 from each breed, were used in this study, whereas 30 birds (10 from each breed) were challenged intranasally with H9N2 virus with a concentration of 106 EID50 at 6wk of age and other half were treated as control. The lung tissues were sampled at 5th day post-infection to study the differential expression of IL-1β, IL-6, and IL-8 using qRT-PCR. Our data revealed a significant difference (P<0.001) in the gene expression levels among all the breeds in response to viral challenge. It was also observed that after exposure to H9N2 virus, Aseel birds showed the highest increase in their expressions of interleukin (IL-1 β, IL-6, and IL-8) genes followed by Naked Neck and WLH respectively suggesting greater susceptibility of Aseel to AIV compared with other breeds. However, these results are in agreement with the severity of disease and incidence of mortality caused by AI in these breeds.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46636089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：