MU Anyanwu, UU Eze, JC Nnamani, NH Ikenna-Ezeh, P. Akpa, OJ Okorie-kanu, B.M. Anene
AbstractThis study was conducted to isolate enterococci from dogs in Nigeria, and to determine the potential virulence, antibiotic susceptibility, phenotypic vancomycin (VAN), high-level ampicillin (AMP) and aminoglycosides susceptibility profile of the isolates. Rectal swabs were collected from 295 randomly-selected, clinically-healthy dogs. The isolation of enterococci was done using Slanetz and Bartley enterococcal selective medium. The resistance of 150 non-repetitive isolates was determined using disc diffusion method. VAN resistance was assessed by high-level disc diffusion and agar-screening methods. High-level AMP and aminoglycosides (gentamicin and streptomycin) resistance was determined by agar-screening method. Potential virulence factors were assayed using phenotypic methods. Out of 295 samples, 234 (80.7%) gave positive growth. From these, 250 enterococcal isolates comprised 229 (91.6%) non-pigmented and 21 (8.4%) pigmented strains, were obtained. Resistance of the isolates was 89% to erythromycin, 92% to rifampicin, 77% to chloramphenicol, 83% to tetracycline, 64% to ciprofloxacin, 32.7% to VAN, 24.7% to high-level streptomycin (HLS) and 6% to high-level gentamicin (HLG). Among 150 non-repetitive resistant isolates, 144 (96%), including all the VAN-, HLS- and HLG-resistant strains, exhibited resistance to at least 3 classes of antibiotics. The mean multiple antibiotic resistance index was 0.54 (range = 0.22 – 0.89). Of these 150 isolates, 94 (62.7%), including all the VAN-, HLS- and HLG-resistant strains, displayed virulence potentials as biofilm (44.7%), surface-layer (13.8%), haemolysin (21.3%), gelatinase (40.4%), caesinase (10.6%) and deoxyribonuclease (12.8%) activities. This study showed that dogs in Nigeria are potential reservoirs and disseminators of potentially-virulent, multidrug-, VAN- and high-level aminoglycosides-resistant enterococci.
{"title":"Occurrence, antibiogram, high-level vancomycin and aminoglycosides resistance and potential virulence factors of enterococci in dogs in Nigeria","authors":"MU Anyanwu, UU Eze, JC Nnamani, NH Ikenna-Ezeh, P. Akpa, OJ Okorie-kanu, B.M. Anene","doi":"10.12681/jhvms.27783","DOIUrl":"https://doi.org/10.12681/jhvms.27783","url":null,"abstract":"AbstractThis study was conducted to isolate enterococci from dogs in Nigeria, and to determine the potential virulence, antibiotic susceptibility, phenotypic vancomycin (VAN), high-level ampicillin (AMP) and aminoglycosides susceptibility profile of the isolates. Rectal swabs were collected from 295 randomly-selected, clinically-healthy dogs. The isolation of enterococci was done using Slanetz and Bartley enterococcal selective medium. The resistance of 150 non-repetitive isolates was determined using disc diffusion method. VAN resistance was assessed by high-level disc diffusion and agar-screening methods. High-level AMP and aminoglycosides (gentamicin and streptomycin) resistance was determined by agar-screening method. Potential virulence factors were assayed using phenotypic methods. Out of 295 samples, 234 (80.7%) gave positive growth. From these, 250 enterococcal isolates comprised 229 (91.6%) non-pigmented and 21 (8.4%) pigmented strains, were obtained. Resistance of the isolates was 89% to erythromycin, 92% to rifampicin, 77% to chloramphenicol, 83% to tetracycline, 64% to ciprofloxacin, 32.7% to VAN, 24.7% to high-level streptomycin (HLS) and 6% to high-level gentamicin (HLG). Among 150 non-repetitive resistant isolates, 144 (96%), including all the VAN-, HLS- and HLG-resistant strains, exhibited resistance to at least 3 classes of antibiotics. The mean multiple antibiotic resistance index was 0.54 (range = 0.22 – 0.89). Of these 150 isolates, 94 (62.7%), including all the VAN-, HLS- and HLG-resistant strains, displayed virulence potentials as biofilm (44.7%), surface-layer (13.8%), haemolysin (21.3%), gelatinase (40.4%), caesinase (10.6%) and deoxyribonuclease (12.8%) activities. This study showed that dogs in Nigeria are potential reservoirs and disseminators of potentially-virulent, multidrug-, VAN- and high-level aminoglycosides-resistant enterococci.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48004591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kiymaci, D. Simsek, KC Tok, D. Dirican, M. Gumustas
One health is an approach that gains importance in the protection of public health and advocates that the environment-animal-human health is linked. Microbial resistance occurring in any of these three factors circulates directly or indirectly between species and affects health. In this study, antimicrobial, anti-quorum sensing activity and potential probiotic properties of Lactobacillus acidophilus and Enterococcus faecalis 1 and 2 isolates from Apis mellifera gut were investigated. Results showed that isolates were found to be resistant to gentamicin, streptomycin, erythromycin, and clindamycin tested. It was determined that isolates were gamma haemolytic and resistant to acid and enzymatic environment conditions. Enterococcus faecalis isolates had antimicrobial activity on Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853 and anti-quorum sensing activity of the culture supernatants of all isolates was detected by a violacein pigment inhibition of Chromobacterium violaceum strain.
{"title":"Probiotic potential and anti-quorum sensing activity of Enterococcus faecalis and Lactobacillus kunkeei isolates from Apis mellifera","authors":"M. Kiymaci, D. Simsek, KC Tok, D. Dirican, M. Gumustas","doi":"10.12681/jhvms.26743","DOIUrl":"https://doi.org/10.12681/jhvms.26743","url":null,"abstract":"One health is an approach that gains importance in the protection of public health and advocates that the environment-animal-human health is linked. Microbial resistance occurring in any of these three factors circulates directly or indirectly between species and affects health. In this study, antimicrobial, anti-quorum sensing activity and potential probiotic properties of Lactobacillus acidophilus and Enterococcus faecalis 1 and 2 isolates from Apis mellifera gut were investigated. Results showed that isolates were found to be resistant to gentamicin, streptomycin, erythromycin, and clindamycin tested. It was determined that isolates were gamma haemolytic and resistant to acid and enzymatic environment conditions. Enterococcus faecalis isolates had antimicrobial activity on Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853 and anti-quorum sensing activity of the culture supernatants of all isolates was detected by a violacein pigment inhibition of Chromobacterium violaceum strain.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46248689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Ozbilek, Y. Ergun, A. Gözer, O. Bahan, S. Alaşahan
The aim of this study was to investigate the effect of the administration of half or full dose of prostaglandinF2α (PGF2α; Gestavet Prost, d-cloprostenol, HIPRA®) injection in a routine synchronization protocol following theend of the breeding period including intravaginal sponge (medroxyprogesterone acetate; Espanjovet, HIPRA®) andeCG (Oviser 500, HIPRA®) on certain fertility parameters in ewes.Two hundred thirty-eight Awassi yearlings (agedone year old) divided into two groups:116 ewes received a half dose (HSDP; 37.5 μg) of PGF2α and 122 ewes receivedfull dose (FSDP;75 μg).The pregnancy rates were 67.2% and 72.1% for HSDP and FSDP, respectively (P>0.05).The lambing rates were 64.7% and 63.9% for HSDP and FSDP, respectively (P>0.05).The single lambing rate inHSDP(94.7%) was higher (P<0.05) compared to that in FSDP (73.1%). The twin and multiple birth rates of FSDPwas higher (P<0.05) than HSDP. As a result, it was inferred that aHSDP could be used in Awassi yearlings withoutexperience of lamb care and in a herd in which single lambing was preferable. Moreover, due to the fact that it is moreaffordable and not detrimentalto fertility, it was concluded that HSDPcan offer an advantage in large flocks and can beused for manipulating fecundity ratesin yearlings.
{"title":"The effect of reduced dose of PGF2α on certain reproductive parameters in awassi yearlings in anestrus synchronisation protocol following the end of the of the breeding season","authors":"I. Ozbilek, Y. Ergun, A. Gözer, O. Bahan, S. Alaşahan","doi":"10.12681/jhvms.27399","DOIUrl":"https://doi.org/10.12681/jhvms.27399","url":null,"abstract":"The aim of this study was to investigate the effect of the administration of half or full dose of prostaglandinF2α (PGF2α; Gestavet Prost, d-cloprostenol, HIPRA®) injection in a routine synchronization protocol following theend of the breeding period including intravaginal sponge (medroxyprogesterone acetate; Espanjovet, HIPRA®) andeCG (Oviser 500, HIPRA®) on certain fertility parameters in ewes.Two hundred thirty-eight Awassi yearlings (agedone year old) divided into two groups:116 ewes received a half dose (HSDP; 37.5 μg) of PGF2α and 122 ewes receivedfull dose (FSDP;75 μg).The pregnancy rates were 67.2% and 72.1% for HSDP and FSDP, respectively (P>0.05).The lambing rates were 64.7% and 63.9% for HSDP and FSDP, respectively (P>0.05).The single lambing rate inHSDP(94.7%) was higher (P<0.05) compared to that in FSDP (73.1%). The twin and multiple birth rates of FSDPwas higher (P<0.05) than HSDP. As a result, it was inferred that aHSDP could be used in Awassi yearlings withoutexperience of lamb care and in a herd in which single lambing was preferable. Moreover, due to the fact that it is moreaffordable and not detrimentalto fertility, it was concluded that HSDPcan offer an advantage in large flocks and can beused for manipulating fecundity ratesin yearlings.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49265322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sahinduran, M. Albay, M. Karaca, MÇ Karakurum, R. Kiyici
Feline Infectious Peritonitis (FIP) is a fatal disease caused by feline coronaviruses. The causative agent is Feline Infectious Peritonitis Virus, a mutation of Feline Enteric Coronavirus. Feline Corona Virus is very common in the cat population. In Feline Corona Virus infected cats, the development of FIP depends on the cat's immune response. FIP disease is more common in young and old cats because young and old animals have a weaker immune system. The acute phase response is a complex systemic reaction that occurs as a response to acute or chronic inflammatory processes such as infection, neoplasia or immunological disorders, tissue damage, trauma and surgery. The study material included 15 cat with presumptive FIP (study group) and 10 healthy cats (control group). Of the 15 cats, 9 (60%) showed dry and 6 (40%) effusive form. Serum amyloid A (SAA), haptoglobin (Hp), α1-acid glycoprotein (AGP), albumin, interleukin-6 (IL-6), hepcidin, alanine-amino transferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen(BUN), and creatinine levels were measured in the serum collected from both groups. There were no difference between the wet and dry FIP in albumin values; haptoglobin, α1-acid glycoprotein, SAA and hepcidin values were significantly different between the two groups. IL-6, showed significant difference in both wet and dry FIP groups. It was also concluded that hepcidin may be used as a biomarker in Feline Infectious Peritonitis disease like other acute phase proteins.
{"title":"Investigation of some acute phase proteins, cytokines and hepcidin values in feline enteric corona virus antibodies and feline infectious peritonitis antigen positive cats","authors":"S. Sahinduran, M. Albay, M. Karaca, MÇ Karakurum, R. Kiyici","doi":"10.12681/jhvms.24162","DOIUrl":"https://doi.org/10.12681/jhvms.24162","url":null,"abstract":"Feline Infectious Peritonitis (FIP) is a fatal disease caused by feline coronaviruses. The causative agent is Feline Infectious Peritonitis Virus, a mutation of Feline Enteric Coronavirus. Feline Corona Virus is very common in the cat population. In Feline Corona Virus infected cats, the development of FIP depends on the cat's immune response. FIP disease is more common in young and old cats because young and old animals have a weaker immune system. The acute phase response is a complex systemic reaction that occurs as a response to acute or chronic inflammatory processes such as infection, neoplasia or immunological disorders, tissue damage, trauma and surgery. The study material included 15 cat with presumptive FIP (study group) and 10 healthy cats (control group). Of the 15 cats, 9 (60%) showed dry and 6 (40%) effusive form. Serum amyloid A (SAA), haptoglobin (Hp), α1-acid glycoprotein (AGP), albumin, interleukin-6 (IL-6), hepcidin, alanine-amino transferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen(BUN), and creatinine levels were measured in the serum collected from both groups. There were no difference between the wet and dry FIP in albumin values; haptoglobin, α1-acid glycoprotein, SAA and hepcidin values were significantly different between the two groups. IL-6, showed significant difference in both wet and dry FIP groups. It was also concluded that hepcidin may be used as a biomarker in Feline Infectious Peritonitis disease like other acute phase proteins.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41625959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mellado, A. Almanza, J. Mellado, Jessica D. Garcia, U. Macías-Cruz, L. Avendaño-Reyes
The seroprevalence of Brucella antibodies in serum of goats and sheep reared on semi-arid pastoral systems of northeastern Mexico was assessed. Additionally, species, gender, and predominant breed were evaluated as risk factors for seropositivity to brucellosis. Data were from 375 flocks of goats or co-mingled goats and sheep. Serum samples from 11001 goats and 4741 sheep collected between 2016 and 2019 were analyzed with the brucellosis card test. The disease affected 12% of flocks. The overall prevalence of brucellosis seropositivity in goats and sheep were estimated at 2.1% (95% CI = 1.78-2.31) and 0.8% (95% CI = 0.58-1.10), respectively. Brucellosis seropositivity was two times more likely (p < 0.01) in goats than sheep. The risk of seropositivity to brucellosis for goats and sheep was 2.3 and 3.2 times higher (p < 0.01) in females than males. Toggenburg goats were more likely (p < 0.01) to be seropositive to brucellosis than all other breeds of goats, whereas criollo sheep were more likely to be seropositive to brucellosis compared to hair sheep composite breeds. The current study revealed that, despite the absence of Brucella vaccination, brucellosis is not widely distributed in the study area. Also of all animals screened, seropositivity to Brucella infection was highest in Toggenburg when compared to other dairy and meat breeds; Criollo sheep also presented the highest seropositivity to brucellosis compared to hair sheep composite breeds. Finally, females compared to males had increased odds of testing positive for brucellosis.
对墨西哥东北部半干旱牧区饲养的山羊和绵羊血清中布鲁氏菌抗体的血清阳性率进行了评估。此外，物种、性别和优势品种被评估为布鲁氏菌病血清阳性的危险因素。数据来自375群山羊或混合山羊和绵羊。对2016年至2019年收集的11001只山羊和4741只绵羊的血清样本进行布鲁氏菌卡试验分析。这种疾病影响了12%的鸡群。山羊和绵羊的布鲁氏菌病血清阳性总体患病率估计分别为2.1% (95% CI = 1.78-2.31)和0.8% (95% CI = 0.58-1.10)。山羊布鲁氏菌病血清阳性的可能性是绵羊的2倍(p < 0.01)。山羊和绵羊的布鲁氏菌病血清阳性风险分别是雄性的2.3倍和3.2倍(p < 0.01)。Toggenburg山羊的布鲁氏菌病血清阳性率高于其他山羊品种(p < 0.01)，而criollo绵羊的布鲁氏菌病血清阳性率高于毛羊复合品种。目前的研究表明，尽管没有布鲁氏菌疫苗接种，但布鲁氏菌病在研究地区并未广泛分布。此外，在所有筛选的动物中，与其他乳制品和肉类品种相比，Toggenburg的布鲁氏菌感染血清阳性反应最高;与毛羊复合品种相比，克里奥罗羊对布鲁氏菌病的血清阳性反应也最高。最后，与男性相比，女性布鲁氏菌病检测呈阳性的几率更高。
{"title":"Sero-epidemiology of brucellosis in small ruminants on rangeland in northern Mexico","authors":"M. Mellado, A. Almanza, J. Mellado, Jessica D. Garcia, U. Macías-Cruz, L. Avendaño-Reyes","doi":"10.12681/jhvms.23288","DOIUrl":"https://doi.org/10.12681/jhvms.23288","url":null,"abstract":"The seroprevalence of Brucella antibodies in serum of goats and sheep reared on semi-arid pastoral systems of northeastern Mexico was assessed. Additionally, species, gender, and predominant breed were evaluated as risk factors for seropositivity to brucellosis. Data were from 375 flocks of goats or co-mingled goats and sheep. Serum samples from 11001 goats and 4741 sheep collected between 2016 and 2019 were analyzed with the brucellosis card test. The disease affected 12% of flocks. The overall prevalence of brucellosis seropositivity in goats and sheep were estimated at 2.1% (95% CI = 1.78-2.31) and 0.8% (95% CI = 0.58-1.10), respectively. Brucellosis seropositivity was two times more likely (p < 0.01) in goats than sheep. The risk of seropositivity to brucellosis for goats and sheep was 2.3 and 3.2 times higher (p < 0.01) in females than males. Toggenburg goats were more likely (p < 0.01) to be seropositive to brucellosis than all other breeds of goats, whereas criollo sheep were more likely to be seropositive to brucellosis compared to hair sheep composite breeds. The current study revealed that, despite the absence of Brucella vaccination, brucellosis is not widely distributed in the study area. Also of all animals screened, seropositivity to Brucella infection was highest in Toggenburg when compared to other dairy and meat breeds; Criollo sheep also presented the highest seropositivity to brucellosis compared to hair sheep composite breeds. Finally, females compared to males had increased odds of testing positive for brucellosis.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46034682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Abo Hashem, H. El-Mahallawy, M. Moursi, R. Abd El-Fattah, M. Enany
Escherichia coli (E. coli) is a zoonotic bacterial pathogen commonly found in human, birds and animals. The widespread use of broad-spectrum beta-lactams for poultry treatment, has resulted in the production of extended spectrum β-lactamases (ESBLs) in E. coli. In this study, we analyzed the antimicrobial resistance pattern of E. coli isolates from broiler chickens and ducks with clinical colibacillosis, and investigated the presence of beta-lactam (blaTEM, blaSHV, ampC, and blaCTX-M) and fluoroquinolone (qnrS and qepA) resistance genes using PCR. E. coli isolates showed a higher resistance against beta-lactams: penicillin G and ceftriaxone (97.5%), cephalexin (92.5%), ampicillin (77.5%), amoxicillin/clavulanic acid (62.5%), with somewhat lower resistance for nalidixic acid (45%), ciprofloxacin (35%), and norfloxacin (32.5%) from fluoroquinolone group. Isolates showed considerable sensitivity to cefotaxime (65%) and levofloxacin (55%). All tested isolates carried blaTEM, blaSHV and ampC resistance genes, and this was associated with the phenotypic resistance too. Although some isolates showed phenotypic intermediate resistance to fluoroquinolones, or resistance to only one agent from this class, the qnrS (83.3%) has been detected with high frequency. The blaCTX-M (27.87%) and qepA (16.7%) showed the lowest occurrence and had been detected in isolates from broiler chickens only. The occurrence of these resistant E. coli serotypes in broiler chickens and ducks pose a potential threat to poultry industry, and constitutes a public health risk to human consumers, slaughterhouse and poultry packing plant workers due to dissemination of beta-lactam and fluoroquinolone resistant E. coli serotypes.
{"title":"Beta-lactam and Fluoroquinolone Resistant Extraintestinal Escherichia coli from Broiler Chickens and Ducks: Public Health Threat","authors":"M. A. Abo Hashem, H. El-Mahallawy, M. Moursi, R. Abd El-Fattah, M. Enany","doi":"10.12681/jhvms.27341","DOIUrl":"https://doi.org/10.12681/jhvms.27341","url":null,"abstract":"Escherichia coli (E. coli) is a zoonotic bacterial pathogen commonly found in human, birds and animals. The widespread use of broad-spectrum beta-lactams for poultry treatment, has resulted in the production of extended spectrum β-lactamases (ESBLs) in E. coli. In this study, we analyzed the antimicrobial resistance pattern of E. coli isolates from broiler chickens and ducks with clinical colibacillosis, and investigated the presence of beta-lactam (blaTEM, blaSHV, ampC, and blaCTX-M) and fluoroquinolone (qnrS and qepA) resistance genes using PCR. E. coli isolates showed a higher resistance against beta-lactams: penicillin G and ceftriaxone (97.5%), cephalexin (92.5%), ampicillin (77.5%), amoxicillin/clavulanic acid (62.5%), with somewhat lower resistance for nalidixic acid (45%), ciprofloxacin (35%), and norfloxacin (32.5%) from fluoroquinolone group. Isolates showed considerable sensitivity to cefotaxime (65%) and levofloxacin (55%). All tested isolates carried blaTEM, blaSHV and ampC resistance genes, and this was associated with the phenotypic resistance too. Although some isolates showed phenotypic intermediate resistance to fluoroquinolones, or resistance to only one agent from this class, the qnrS (83.3%) has been detected with high frequency. The blaCTX-M (27.87%) and qepA (16.7%) showed the lowest occurrence and had been detected in isolates from broiler chickens only. The occurrence of these resistant E. coli serotypes in broiler chickens and ducks pose a potential threat to poultry industry, and constitutes a public health risk to human consumers, slaughterhouse and poultry packing plant workers due to dissemination of beta-lactam and fluoroquinolone resistant E. coli serotypes.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49190528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Agudelo-Yepes, J. Puerta-Suárez, D.F. Carrillo-Gonzalez, W. C. Cardona Maya
The objective of this work was to determine the frequency of bacteriospermia and its effect on the seminal quality in canines. Dogs were divided into two groups according to weight: small dogs between 1 and 10 kg (Group 1) and dogs with more than 10 kg (Group 2). The foreskin was disinfected in each animal (n=15), and the semen sample was collected by the gloved hand method. Sperm motility, morphology, viability, and concentration were evaluated using a 40x microscope. A 10µL of semen drop was cultured by diffusion method on blood agar and MacConkey agar. Colony-forming units (CFU) were quantitatively evaluated, and biochemical identification was carried out after 48 hours at 37°C. Conventional PCR was performed on the semen samples to evaluate the presence/absence of 11 bacteria. Bacterial growth was found in all samples. The CFU/mL in blood agar were 34042.8 for group 1 and 107714.3 for group 2, while on MacConkey agar were 142.9 CFU/mL, and 21328.6 CFU/mL, respectively. Coagulase-negative Staphylococcus was the most frequent bacteria isolated by conventional culture (64.3%), and Staphylococcus aureus and Klebsiella spp. were the most common bacteria found by conventional PCR. Between both groups, only a statistically significant difference was found in normal sperm morphology. A negative correlation was observed between viability and morphology with CFU on MacConkey agar. One dog was ruled out because he had azoospermia. Canine seminal bacteriospermia is quite frequent and could alter its quality. The presence of Gram-negative bacteria is associated with greater alteration in the semen analysis.
{"title":"Bacteriospermia assessment and its relationship with conventional seminal parameters in stud dogs ejaculates (Canis familiaris)","authors":"S. Agudelo-Yepes, J. Puerta-Suárez, D.F. Carrillo-Gonzalez, W. C. Cardona Maya","doi":"10.12681/jhvms.27558","DOIUrl":"https://doi.org/10.12681/jhvms.27558","url":null,"abstract":"The objective of this work was to determine the frequency of bacteriospermia and its effect on the seminal quality in canines. Dogs were divided into two groups according to weight: small dogs between 1 and 10 kg (Group 1) and dogs with more than 10 kg (Group 2). The foreskin was disinfected in each animal (n=15), and the semen sample was collected by the gloved hand method. Sperm motility, morphology, viability, and concentration were evaluated using a 40x microscope. A 10µL of semen drop was cultured by diffusion method on blood agar and MacConkey agar. Colony-forming units (CFU) were quantitatively evaluated, and biochemical identification was carried out after 48 hours at 37°C. Conventional PCR was performed on the semen samples to evaluate the presence/absence of 11 bacteria. Bacterial growth was found in all samples. The CFU/mL in blood agar were 34042.8 for group 1 and 107714.3 for group 2, while on MacConkey agar were 142.9 CFU/mL, and 21328.6 CFU/mL, respectively. Coagulase-negative Staphylococcus was the most frequent bacteria isolated by conventional culture (64.3%), and Staphylococcus aureus and Klebsiella spp. were the most common bacteria found by conventional PCR. Between both groups, only a statistically significant difference was found in normal sperm morphology. A negative correlation was observed between viability and morphology with CFU on MacConkey agar. One dog was ruled out because he had azoospermia. Canine seminal bacteriospermia is quite frequent and could alter its quality. The presence of Gram-negative bacteria is associated with greater alteration in the semen analysis.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45081013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salmonella enterica is an important agent of food borne illnesses in humans. Poultry meat and products are easily contaminated with different strains of Salmonella enterica. Consumption of such food causes salmonellosis in human. The main aim of present investigation was to survey the prevalence of virulence associated genes of Salmonella enterica isolated from fresh chicken meat available in retail shops and markets at District Peshawar, Pakistan. Fresh chicken meat samples were collected from retail shops and markets at District Peshawar, Pakistan. The samples were processed for isolation of Salmonella enterica using selective media. Presumptive isolates were confirmed as Salmonella enterica via different biochemical tests and by amplification of invA gene. Isolates were tested for the presence of eight different virulence genes invA, spiA, cdtB, prgH, orgA, lpfC, sopB, and pefA. Amongst all isolates, 90(100%) had invA gene, 44(48.8%) had lfpC gene, 40(44.4%) had sopB gene, 27(30%) had orgA gene, 24(26.6%) hand spiA gene, 19(21.1%) had pefA gene, 18(20%) had prgH gene and 6(6.6%) had cdtB gene. 15.5% of the isolates had five virulence genes and 21.1% had four virulence genes. The findings of this study highlight an alarming public health risk and demand for strict bio-safety hygiene measures to be taken.
{"title":"Molecular identification of virulence features of Salmonella enterica isolated from fresh chicken meat sold at retail shops and markets in Peshawar, Pakistan","authors":"Shaista Rahat, K. Ahmad, Amjad Ali","doi":"10.12681/jhvms.27474","DOIUrl":"https://doi.org/10.12681/jhvms.27474","url":null,"abstract":"Salmonella enterica is an important agent of food borne illnesses in humans. Poultry meat and products are easily contaminated with different strains of Salmonella enterica. Consumption of such food causes salmonellosis in human. The main aim of present investigation was to survey the prevalence of virulence associated genes of Salmonella enterica isolated from fresh chicken meat available in retail shops and markets at District Peshawar, Pakistan. Fresh chicken meat samples were collected from retail shops and markets at District Peshawar, Pakistan. The samples were processed for isolation of Salmonella enterica using selective media. Presumptive isolates were confirmed as Salmonella enterica via different biochemical tests and by amplification of invA gene. Isolates were tested for the presence of eight different virulence genes invA, spiA, cdtB, prgH, orgA, lpfC, sopB, and pefA. Amongst all isolates, 90(100%) had invA gene, 44(48.8%) had lfpC gene, 40(44.4%) had sopB gene, 27(30%) had orgA gene, 24(26.6%) hand spiA gene, 19(21.1%) had pefA gene, 18(20%) had prgH gene and 6(6.6%) had cdtB gene. 15.5% of the isolates had five virulence genes and 21.1% had four virulence genes. The findings of this study highlight an alarming public health risk and demand for strict bio-safety hygiene measures to be taken.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45645971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışma, Kahramanmaraş, Türkiye bölgesindeki kafes çiftliklerinde kültürlenen gökkuşağı alabalığındaki Vibrio anguillarum suşlarının fenotipik ve genotipik özelliklerinin izolasyonu ve incelenmesi ile ilgilidir . Bu amaç için, karaciğer, dalak, böbrek ve bağırsak örnekleri fenotipik özelliklerini ve biyokimyasal kimlik incelemek üzere Haziran 2014 farklı kafes çiftliklerinde yaklaşık 250-300 g ağırlığında alabalık farklı gökkuşağı alınmıştır V. anguillarum , Biolog Sistemi, gökkuşağı alabalığından alınan örneklerden elde edilen saf vibrio suşlarına uygulandı. 20 V. anguillarumu doğrulamak için spesifik bir primer ile polimeraz zincir reaksiyon tekniği kullanıldıfenotipik ve biyokimyasal özelliklere göre belirlenen suşlar. Daha sonra, bunların hepsinin V. anguillarum olduğu doğrulandı . Örneklerden elde edilen V. anguillarum suşlarının sekans analizi gerçekleştirildi ve daha sonra bunların karşılık gelen verileri yorumlandı. Bu çalışmanın sonucunda V. anguillarum bakterileri iki farklı alabalık kafesi çiftliğinde tespit edilmiştir. V. anguillarum'un tanımlanma süresi kültür yöntemi ile üç hafta sürerken , kısa sürede PCR tekniği ile yapıldı.
本研究涉及土耳其咖啡因农场中鳗弧菌物种的表型和基因型特性的分离和分析。为此，2014年6月，在大约250至300克不同的咖啡因农场采集了具有表型特征和生物神话特征的肝脏、肌肉、肾脏和肩部样本，其中包括从阴影中采集的各种结核病样本，包括anguillarum、Biolog系统。为了验证20 V anguillars，根据表型和生物学特性定义了聚合酶链式反应技术。后来，我们确认所有这些都是V角。Örneklerden elde edilen V.anguillarum suşlarının sekans analizi gerçekleştirildi ve daha sonra bunların karşılık gelen verileri yorumlandı。这项工作的结果是，在两个不同的头痛农场中发现了V角细菌。只要通过培养方法对鳗弧菌进行三周的鉴定，PCR就很快发展起来。
{"title":"Investigation of phenotypic and genotypic characteristics of isolated Vibrio anguillarum strains in rainbow trout cage farms in Kahramanmaraş city of Turkey","authors":"M. Özcan, E. Donat","doi":"10.12681/jhvms.22851","DOIUrl":"https://doi.org/10.12681/jhvms.22851","url":null,"abstract":"Bu çalışma, Kahramanmaraş, Türkiye bölgesindeki kafes çiftliklerinde kültürlenen gökkuşağı alabalığındaki Vibrio anguillarum suşlarının fenotipik ve genotipik özelliklerinin izolasyonu ve incelenmesi ile ilgilidir . Bu amaç için, karaciğer, dalak, böbrek ve bağırsak örnekleri fenotipik özelliklerini ve biyokimyasal kimlik incelemek üzere Haziran 2014 farklı kafes çiftliklerinde yaklaşık 250-300 g ağırlığında alabalık farklı gökkuşağı alınmıştır V. anguillarum , Biolog Sistemi, gökkuşağı alabalığından alınan örneklerden elde edilen saf vibrio suşlarına uygulandı. 20 V. anguillarumu doğrulamak için spesifik bir primer ile polimeraz zincir reaksiyon tekniği kullanıldıfenotipik ve biyokimyasal özelliklere göre belirlenen suşlar. Daha sonra, bunların hepsinin V. anguillarum olduğu doğrulandı . Örneklerden elde edilen V. anguillarum suşlarının sekans analizi gerçekleştirildi ve daha sonra bunların karşılık gelen verileri yorumlandı. Bu çalışmanın sonucunda V. anguillarum bakterileri iki farklı alabalık kafesi çiftliğinde tespit edilmiştir. V. anguillarum'un tanımlanma süresi kültür yöntemi ile üç hafta sürerken , kısa sürede PCR tekniği ile yapıldı. ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42826833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was conducted to determine the impact of chitosan on lead (Pb)-induced hepatotoxicity. Thus, lead acetate was administered intraperitoneally (50 mg/kg for 5 days) and chitosan was given as 200 mg/kg via oral gavage for 28 days. When the trial was terminated, it was determined that aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in serum as well as Pb, 8-hydroxy-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels and catalase (CAT) activity in liver tissue significantly increased, however reduced glutathione (GSH) and ceruloplasmin (Cp) levels in liver and high density lipoproteins (HDL) in serum had a significant decrease in the Pb group when compared to the control group. The administration of chitosan significantly prevented Pb-induced changes in serum liver enzyme activities, 8-OHdG and MDA levels. In addition, chitosan showed a statistically insignificant effect on reduced GSH, Cp, HDL levels and CAT activity. Accordingly, administration of chitosan can strengthen the antioxidant defence system of liver tissue and may decrease oxidative stress.
{"title":"The Impact of Chitosan on Oxidative Stress in Liver of Rats Loaded With Lead Acetate","authors":"U. Ozdek, H. Toz, AU Kömüroğlu, L. Mis, Y. Değer","doi":"10.12681/jhvms.24541","DOIUrl":"https://doi.org/10.12681/jhvms.24541","url":null,"abstract":"This study was conducted to determine the impact of chitosan on lead (Pb)-induced hepatotoxicity. Thus, lead acetate was administered intraperitoneally (50 mg/kg for 5 days) and chitosan was given as 200 mg/kg via oral gavage for 28 days. When the trial was terminated, it was determined that aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in serum as well as Pb, 8-hydroxy-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels and catalase (CAT) activity in liver tissue significantly increased, however reduced glutathione (GSH) and ceruloplasmin (Cp) levels in liver and high density lipoproteins (HDL) in serum had a significant decrease in the Pb group when compared to the control group. The administration of chitosan significantly prevented Pb-induced changes in serum liver enzyme activities, 8-OHdG and MDA levels. In addition, chitosan showed a statistically insignificant effect on reduced GSH, Cp, HDL levels and CAT activity. Accordingly, administration of chitosan can strengthen the antioxidant defence system of liver tissue and may decrease oxidative stress.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46177191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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