The aim of this study is to investigate the presence of BEF disease in the blood, sera and tissue samples sent to the Viral Diagnosis Laboratory of Adana Veterinary Control Institute with suspected BEF in 2020 from different provinces of Turkey as well as to perform characterization and phylogenetic analysis of cases tested positive with molecular methods. For this purpose, 79 defibrinated blood and 2 tissue samples as well as 168 sera samples obtained from cattle varying in breed, age, and sex, were examined by using Real Time RT-PCR and Blocking ELISA, respectively. Two new degenerate primers amplifying 956 bp of the protein G (AVKEF_AATGTTCCNGTGAATTGTGGAG and AVKER_TGCATAATCYCTTCCTGGTCT) were designed and the phylogenetic analysis of the samples tested positive with RT-PCR was performed. As a result of the examinations conducted, 64.20% (52/81) of the defibrinated blood and tissue samples, and 69.04% (116/168) of the sera samples were diagnosed as positive. Through VERO cell culture, the virus was isolated from the tissue samples of 2 animals in Adana and Sanliurfa provinces, which were positive. The phylogenetic analysis revealed that the virus circulated in Turkey during the 2020 pandemic belongs to the Middle East lineage. We concluded that monitoring the disease and identifying the lineages of the virus that have caused pandemic are significant in the selection of the proper vaccine and the disease control.
{"title":"Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020","authors":"BS Tokgoz, EA Tokgoz, MA Sözmen, O. Avci, AE Ütük","doi":"10.12681/jhvms.31543","DOIUrl":"https://doi.org/10.12681/jhvms.31543","url":null,"abstract":"The aim of this study is to investigate the presence of BEF disease in the blood, sera and tissue samples sent to the Viral Diagnosis Laboratory of Adana Veterinary Control Institute with suspected BEF in 2020 from different provinces of Turkey as well as to perform characterization and phylogenetic analysis of cases tested positive with molecular methods. For this purpose, 79 defibrinated blood and 2 tissue samples as well as 168 sera samples obtained from cattle varying in breed, age, and sex, were examined by using Real Time RT-PCR and Blocking ELISA, respectively. Two new degenerate primers amplifying 956 bp of the protein G (AVKEF_AATGTTCCNGTGAATTGTGGAG and AVKER_TGCATAATCYCTTCCTGGTCT) were designed and the phylogenetic analysis of the samples tested positive with RT-PCR was performed. As a result of the examinations conducted, 64.20% (52/81) of the defibrinated blood and tissue samples, and 69.04% (116/168) of the sera samples were diagnosed as positive. Through VERO cell culture, the virus was isolated from the tissue samples of 2 animals in Adana and Sanliurfa provinces, which were positive. The phylogenetic analysis revealed that the virus circulated in Turkey during the 2020 pandemic belongs to the Middle East lineage. We concluded that monitoring the disease and identifying the lineages of the virus that have caused pandemic are significant in the selection of the proper vaccine and the disease control.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was undertaken to examine genetic polymorphisms of Alpha-S1 casein (CSN1S1) and β-Lactoglobulin (BLG) genes in Norduz sheep by PCR-RFLP method. In the study, 10 ml blood samples were taken from 102 heads of Norduz sheep into tubes with EDTA. DNA isolation from blood samples was done with ready-made commercial kits. The target DNA region of alpha-s1 casein and beta-lactoglobulin genes was amplified by PCR technique. The obtained PCR patterns were digested with MboII and RsaI restriction enzymes to determine the genotypes of Alpha-S1 casein and β-lactoglobulin genes, respectively. The cut fragments were run on 2% agarose gel electrophoresis and genotypes were determined under UV light. Statistical analysis, after calculating allele and genotype frequencies by direct gene counting method, the distribution of observed and expected genotypic frequencies was determined according to the Hardy-Weinberg Equilibrium, and whether it was compatible with the χ2 test. β-Lactoglobulin AA, AB and BB genotype frequencies were found to be 17.6%, 69.6 and 12.7%, respectively. It was determined that the β-Lactoglobulin gene was not in Hardy-Weinberg Equilibrium. AlphaS1-Casein AA, AC and CC genotype frequencies were determined as 0.0, 2.9 and 97.1%, respectively. The Alpha-S1 casein gene was determined to be in Hardy-Weinberg equilibrium. It was determined that Norduz sheep showed polymorphism in terms of Alpha-S1 Casein and β-Lactoglobulin genes. In conclusion, it was determined that the A allele of the β-Lactoglobulin gene and the AB genotype were more common, the C allele and the CC genotype of the Alpha-S1 casein gene were more common, and the AA genotype was absent in Norduz sheep. It was concluded that it would be useful to investigate the economic effects of genetic polymorphisms of Alpha-S1 casein and β-Lactoglobulin genes on various yield traits in larger Norduz sheep populations.
本研究采用 PCR-RFLP 方法检测诺尔杜兹羊α-S1 酪蛋白（CSN1S1）和β-乳球蛋白（BLG）基因的遗传多态性。在研究中，从 102 只诺杜兹绵羊的头部抽取 10 毫升血液样本，装入装有乙二胺四乙酸（EDTA）的试管中。使用现成的商业试剂盒从血样中分离 DNA。用 PCR 技术扩增α-s1 酪蛋白基因和β-乳球蛋白基因的目标 DNA 区域。用 MboII 和 RsaI 限制性酶对 PCR 图谱进行消化，以分别确定α-S1 酪蛋白基因和 β-乳球蛋白基因的基因型。切割后的片段在 2% 琼脂糖凝胶电泳中运行，并在紫外光下测定基因型。统计分析：用基因直接计数法计算等位基因和基因型频率后，根据哈代-温伯格平衡（Hardy-Weinberg Equilibrium）确定观察到的和预期的基因型频率分布，并进行χ2检验是否符合。结果发现，β-乳球蛋白 AA、AB 和 BB 基因型频率分别为 17.6%、69.6% 和 12.7%。结果表明，β-乳球蛋白基因不处于哈代-温伯格平衡状态。阿尔法-S1-酪蛋白 AA、AC 和 CC 基因型频率分别为 0.0、2.9 和 97.1%。经测定，Alpha-S1-酪蛋白基因处于哈代-温伯格平衡状态。结果表明，诺尔杜兹羊在α-S1酪蛋白基因和β-乳球蛋白基因方面存在多态性。总之，在诺杜兹羊中，β-乳球蛋白基因的 A 等位基因和 AB 基因型较为常见，α-S1 酪蛋白基因的 C 等位基因和 CC 基因型较为常见，而 AA 基因型则不存在。结论是，在较大的诺杜兹绵羊种群中研究 Alpha-S1 酪蛋白基因和 β-乳球蛋白基因的遗传多态性对各种产量性状的经济影响是有益的。
{"title":"Investigation of Genetic Polymorphisms of CSN1S1 and BLG Genes in Norduz sheep by PCR-RFLP method","authors":"B. Çak, O. Yılmaz, AF Demirel","doi":"10.12681/jhvms.31872","DOIUrl":"https://doi.org/10.12681/jhvms.31872","url":null,"abstract":"This study was undertaken to examine genetic polymorphisms of Alpha-S1 casein (CSN1S1) and β-Lactoglobulin (BLG) genes in Norduz sheep by PCR-RFLP method. In the study, 10 ml blood samples were taken from 102 heads of Norduz sheep into tubes with EDTA. DNA isolation from blood samples was done with ready-made commercial kits. The target DNA region of alpha-s1 casein and beta-lactoglobulin genes was amplified by PCR technique. The obtained PCR patterns were digested with MboII and RsaI restriction enzymes to determine the genotypes of Alpha-S1 casein and β-lactoglobulin genes, respectively. The cut fragments were run on 2% agarose gel electrophoresis and genotypes were determined under UV light. Statistical analysis, after calculating allele and genotype frequencies by direct gene counting method, the distribution of observed and expected genotypic frequencies was determined according to the Hardy-Weinberg Equilibrium, and whether it was compatible with the χ2 test. β-Lactoglobulin AA, AB and BB genotype frequencies were found to be 17.6%, 69.6 and 12.7%, respectively. It was determined that the β-Lactoglobulin gene was not in Hardy-Weinberg Equilibrium. AlphaS1-Casein AA, AC and CC genotype frequencies were determined as 0.0, 2.9 and 97.1%, respectively. The Alpha-S1 casein gene was determined to be in Hardy-Weinberg equilibrium. It was determined that Norduz sheep showed polymorphism in terms of Alpha-S1 Casein and β-Lactoglobulin genes. In conclusion, it was determined that the A allele of the β-Lactoglobulin gene and the AB genotype were more common, the C allele and the CC genotype of the Alpha-S1 casein gene were more common, and the AA genotype was absent in Norduz sheep. It was concluded that it would be useful to investigate the economic effects of genetic polymorphisms of Alpha-S1 casein and β-Lactoglobulin genes on various yield traits in larger Norduz sheep populations.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Channo, A. Kaka, AA Memon, MC Malhi, M. Bakhsh, Q. Kalwar, DH Kalhoro, A. Sethar, RA Jariko
In this experiment, four healthy Tharparkar Cattle Bulls, 4-5 years aged were used. Total 52 (n=13) ejaculates were collected, and the semen samples having motility, morphology and viability ≥70% were processed and extended into BIOXcellTM and Tris Based Egg Yolk extender supplemented with and without α-tocopherol (Vitamin E) 0.02 Mm/ml. Pre-thawed assessment of motility, morphology, viability and membrane integrity with BIOXcellTM + α-tocopherol (Vitamin E) (79.09±0.87, 86.13±0.79, 82.13±1.20, 81.40±0.77) showed improved quality parameters as compared to Tris+ α-tocopherol (Vitamin E) (74.69±0.94, 82.81±1.00, 77.63±1.26, 77.31±1.01), BIOXcellTM (73.55±1.20, 80.81±0.96, 76.02±1.28, 75.31±1.20) and Tris (71.72±1.28, 78.90±1.01, 74.04±1.31, 73.18±1.29) respectively. In conclusion supplementation of α-tocopherol (Vitamin E) improves the Pre thawed quality parameters i.e. Motility, Morphology, Viability and Membrane Integrity of Tharparkar Bull semen extended in BIOXcellTM and Tris Based Egg Yolk extender.
{"title":"Effects of α-Tocopherol on chilled quality parameters of Tharparkar bull semen extended in Lecithin and Tris based egg yolk extender","authors":"A. Channo, A. Kaka, AA Memon, MC Malhi, M. Bakhsh, Q. Kalwar, DH Kalhoro, A. Sethar, RA Jariko","doi":"10.12681/jhvms.31491","DOIUrl":"https://doi.org/10.12681/jhvms.31491","url":null,"abstract":"In this experiment, four healthy Tharparkar Cattle Bulls, 4-5 years aged were used. Total 52 (n=13) ejaculates were collected, and the semen samples having motility, morphology and viability ≥70% were processed and extended into BIOXcellTM and Tris Based Egg Yolk extender supplemented with and without α-tocopherol (Vitamin E) 0.02 Mm/ml. Pre-thawed assessment of motility, morphology, viability and membrane integrity with BIOXcellTM + α-tocopherol (Vitamin E) (79.09±0.87, 86.13±0.79, 82.13±1.20, 81.40±0.77) showed improved quality parameters as compared to Tris+ α-tocopherol (Vitamin E) (74.69±0.94, 82.81±1.00, 77.63±1.26, 77.31±1.01), BIOXcellTM (73.55±1.20, 80.81±0.96, 76.02±1.28, 75.31±1.20) and Tris (71.72±1.28, 78.90±1.01, 74.04±1.31, 73.18±1.29) respectively. In conclusion supplementation of α-tocopherol (Vitamin E) improves the Pre thawed quality parameters i.e. Motility, Morphology, Viability and Membrane Integrity of Tharparkar Bull semen extended in BIOXcellTM and Tris Based Egg Yolk extender.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Honey and other bee products have been extensively researched in recent years due to the demand for natural products with nutritional and therapeutic qualities, as they contain high levels of biologically active compounds and valuable nutrients. In this study, antioxidant, anti-inflammatory and antimicrobial activities of bee bread and propolis samples formed after colony development by bees of different races and ecotypes accepted to core colonies in the same location were evaluated. Total phenolic, flavonoid and antioxidant content values were the the highest in propolis samples, especially in propolis from Apis mellifera anatoliaca. Values of this type gave the most effective results in almost all of the other tests performed, such as DPPH free radical scavenging, lipid peroxidation inhibition, anti-inflammatory and antimicrobial activity. In addition, the amount of phenolics detected in the phenolic component analysis of this species is higher. It was surprising that propolis samples obtained from Efe and Muğla ecotypes showed higher values, respectively, in the test for chelating activity with Fe. The fact that the values calculated for the bee breads were lower in all cases compared to the propolis samples, the highest values were calculated for the bee bread harvested from the efe ecotype, in contrast to the propolis ecotypes. None of the tested bee breads showed antimicrobial activity on selected pathogenic bacteria. Thus, with this study, it was evaluated how the differences arising from both race and ecotype and the type of bee product reflected on biological activities.
{"title":"Comparison of some biological activities of propolis and bee bread samples obtained from Apis mellifera Anatoliaca and its Muğla and Efe ecotypes","authors":"D. Kabakci","doi":"10.12681/jhvms.30859","DOIUrl":"https://doi.org/10.12681/jhvms.30859","url":null,"abstract":"Honey and other bee products have been extensively researched in recent years due to the demand for natural products with nutritional and therapeutic qualities, as they contain high levels of biologically active compounds and valuable nutrients. In this study, antioxidant, anti-inflammatory and antimicrobial activities of bee bread and propolis samples formed after colony development by bees of different races and ecotypes accepted to core colonies in the same location were evaluated. Total phenolic, flavonoid and antioxidant content values were the the highest in propolis samples, especially in propolis from Apis mellifera anatoliaca. Values of this type gave the most effective results in almost all of the other tests performed, such as DPPH free radical scavenging, lipid peroxidation inhibition, anti-inflammatory and antimicrobial activity. In addition, the amount of phenolics detected in the phenolic component analysis of this species is higher. It was surprising that propolis samples obtained from Efe and Muğla ecotypes showed higher values, respectively, in the test for chelating activity with Fe. The fact that the values calculated for the bee breads were lower in all cases compared to the propolis samples, the highest values were calculated for the bee bread harvested from the efe ecotype, in contrast to the propolis ecotypes. None of the tested bee breads showed antimicrobial activity on selected pathogenic bacteria. Thus, with this study, it was evaluated how the differences arising from both race and ecotype and the type of bee product reflected on biological activities.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to evaluate the oxidative stress and inflammation status of ad libitum whey consumption as drinking water on fatting Merino lambs (n=24). Lambs were randomly divided into two groups and received ad libitum freshwater or whey enriched water and had clover as roughage and lamb grower feed as concentrate daily. Blood samples were collected before the trial (T1), on the 15th (T2) and 30th (T3) days and analyses were conducted on hemogram, serum antioxidant enzymes (Superoxide dismutase, SOD; Glutathione peroxidase, GSH-Px) inflammation response (C-Reactive Protein, CRP), complement activation, and chemokine marker (complement component 4, C4), lymphocyte activation factor (Interleukin 1 beta, IL-1 β), systemic inflammation cytokine (Tumor necrosis factor-alpha, TNF-α), lipid peroxidation marker (Malondialdehyde, MDA). The rate of hematocrit was found to be higher in the experimental group in T3 (35.55 ± 10.54) compared to the control group (44.50 ± 2.58). The platelet amounts were found to be higher in the whey group in T2 and T3 compared to the control group (p<0.05). In conclusion, with the use of whey protein-enriched drinking water, no significant change was observed in the amounts of TNF-α, SOD, MDA, IL-1β, C4, CRP under these study conditions.
{"title":"Effects of whey-enriched drinking water on fattening merino lamb growth, hemogram, inflammation, oxidant and antioxidant parameters","authors":"E. Danyer, T. Bilal, A. Altiner, H. Eseceli","doi":"10.12681/jhvms.31439","DOIUrl":"https://doi.org/10.12681/jhvms.31439","url":null,"abstract":"This study aimed to evaluate the oxidative stress and inflammation status of ad libitum whey consumption as drinking water on fatting Merino lambs (n=24). Lambs were randomly divided into two groups and received ad libitum freshwater or whey enriched water and had clover as roughage and lamb grower feed as concentrate daily. Blood samples were collected before the trial (T1), on the 15th (T2) and 30th (T3) days and analyses were conducted on hemogram, serum antioxidant enzymes (Superoxide dismutase, SOD; Glutathione peroxidase, GSH-Px) inflammation response (C-Reactive Protein, CRP), complement activation, and chemokine marker (complement component 4, C4), lymphocyte activation factor (Interleukin 1 beta, IL-1 β), systemic inflammation cytokine (Tumor necrosis factor-alpha, TNF-α), lipid peroxidation marker (Malondialdehyde, MDA). The rate of hematocrit was found to be higher in the experimental group in T3 (35.55 ± 10.54) compared to the control group (44.50 ± 2.58). The platelet amounts were found to be higher in the whey group in T2 and T3 compared to the control group (p<0.05). In conclusion, with the use of whey protein-enriched drinking water, no significant change was observed in the amounts of TNF-α, SOD, MDA, IL-1β, C4, CRP under these study conditions.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried out to investigate the effects on carcass characteristics of lambs fed with different silage types (corn silage, sunflower silage and their mixtures) and their mixtures at different levels. In the study, rumen volatile fatty acids levels and rumen pH of the lambs used in the experiment were determined at the beginning and end of fattening. For corn silage and sunflower silages, aerobic stability and lactic acid bacteria and yeast-mold counts were determined. In the treatment, 40 Kıvırcık male lambs aged 2.5-3 months were used as animal material. Lambs were divided into 5 different silage groups (100% corn silage, 75% corn+25% sunflower silage, 50% corn silage+50% sunflower silage, 25% corn silage+75% sunflower silage, 100% sunflower silage) and it was planned to have 8 lambs in each group. The lambs were housed in individual compartments during the experiment and the animals were individually fed. Silages were given to the lambs ad libitum and in addition to silage 700, 900 and 1400 g concentrated feed were given daily between 0-21, 21-42 and 42-56 days, respectively. As a result, it can be said that feeding with different silage types does not have a significant effect on carcass characteristics of lambs, and silages prepared with corn silage, sunflower silage and their mixtures can be used successfully in lamb fattening.
{"title":"The effect on carcass characteristics of different silage types used in the rations of fattening lambs","authors":"Ö. Şengül, İ. Ak","doi":"10.12681/jhvms.31705","DOIUrl":"https://doi.org/10.12681/jhvms.31705","url":null,"abstract":"This study was carried out to investigate the effects on carcass characteristics of lambs fed with different silage types (corn silage, sunflower silage and their mixtures) and their mixtures at different levels. In the study, rumen volatile fatty acids levels and rumen pH of the lambs used in the experiment were determined at the beginning and end of fattening. For corn silage and sunflower silages, aerobic stability and lactic acid bacteria and yeast-mold counts were determined. In the treatment, 40 Kıvırcık male lambs aged 2.5-3 months were used as animal material. Lambs were divided into 5 different silage groups (100% corn silage, 75% corn+25% sunflower silage, 50% corn silage+50% sunflower silage, 25% corn silage+75% sunflower silage, 100% sunflower silage) and it was planned to have 8 lambs in each group. The lambs were housed in individual compartments during the experiment and the animals were individually fed. Silages were given to the lambs ad libitum and in addition to silage 700, 900 and 1400 g concentrated feed were given daily between 0-21, 21-42 and 42-56 days, respectively. As a result, it can be said that feeding with different silage types does not have a significant effect on carcass characteristics of lambs, and silages prepared with corn silage, sunflower silage and their mixtures can be used successfully in lamb fattening. ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objectives of this study were to investigate the effects of dietary supplementation of Suaeda rigida extract on the growth performance, antioxidant capacity and immune function of Karakul sheep. Blood samples were collected from the jugular veins before morning feeding on the 10th, 20th and 30th days of the trial period, respectively, and the serum antioxidant and immune indexes of Karakul sheep in the control and experimental groups were determined. The results showed that the growth performance of Karakul sheep was dramatically improved by dietary supplementation of Suaeda rigida, the contents of NO, cGMP, MDA, H2O2, •OH and the activities of XOD, GGT, NOS in the serums of Karakul sheep were inhibited to varying degrees, the GSH content and the activities of GSH-Px, GST, CAT and SOD were substantially increased (P<0.05 at 10d, P<0.01 both 20d and 30d). At 30 days, the contents of total protein, globulin, calcium and three immunoglobulins including IgG, IgA, IgM in the sera of experimental Karakul sheep were significantly higher than those in the control group (P<0.05). All the results suggested that the growth performance, antioxidant, anti-aging capacity, immune function, and life extension of Karakul sheep were significantly improved by dietary supplementation with Suaeda rigida.
{"title":"Effects of Suaeda rigida extract on the growth performance, antioxidant capacity and immune function of Karakul sheep","authors":"A. Sha, Y. Liu, H. Hao","doi":"10.12681/jhvms.30933","DOIUrl":"https://doi.org/10.12681/jhvms.30933","url":null,"abstract":"The objectives of this study were to investigate the effects of dietary supplementation of Suaeda rigida extract on the growth performance, antioxidant capacity and immune function of Karakul sheep. Blood samples were collected from the jugular veins before morning feeding on the 10th, 20th and 30th days of the trial period, respectively, and the serum antioxidant and immune indexes of Karakul sheep in the control and experimental groups were determined. The results showed that the growth performance of Karakul sheep was dramatically improved by dietary supplementation of Suaeda rigida, the contents of NO, cGMP, MDA, H2O2, •OH and the activities of XOD, GGT, NOS in the serums of Karakul sheep were inhibited to varying degrees, the GSH content and the activities of GSH-Px, GST, CAT and SOD were substantially increased (P<0.05 at 10d, P<0.01 both 20d and 30d). At 30 days, the contents of total protein, globulin, calcium and three immunoglobulins including IgG, IgA, IgM in the sera of experimental Karakul sheep were significantly higher than those in the control group (P<0.05). All the results suggested that the growth performance, antioxidant, anti-aging capacity, immune function, and life extension of Karakul sheep were significantly improved by dietary supplementation with Suaeda rigida.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Epilepsy is a neurological disease that results from an abnormality in the brain's activity. Phosphatidylinositol-3-kinase (PI3K) signaling pathway has played a crucial role in epilepsy pathogenesis. Alpelisib (ALP) is a selective inhibitor of PI3K. We examined the ability of ALP to treat pentylenetetrazole (PTZ)-induced convulsions in a rat model. Male Wistar rats (200-250 g, 8 weeks old) were injected intraperitoneally (IP) with ALP at different doses of 15 and 30 mg/kg, or vehicle 30 min prior to PTZ (70 mg/kg, IP) treatment. Racine's scale was used to assess behavioral seizures. The results showed that pretreatment with ALP decreased the seizure stages according to the Racine scale, significantly prolonged the duration of general tonic-clonic seizure (GTCS) and reduced the number of myoclonic jerks (P < 0.05). In conclusion, based on results it was shown that PI3K antagonist ALP inhibited PTZ-induced seizure by inhibiting the PI3K signaling pathway via ALP.
癫痫是一种因大脑活动异常而导致的神经系统疾病。磷脂酰肌醇-3-激酶（PI3K）信号通路在癫痫发病机制中起着至关重要的作用。Alpelisib（ALP）是PI3K的一种选择性抑制剂。我们研究了 ALP 在大鼠模型中治疗戊四唑（PTZ）诱导的惊厥的能力。雄性 Wistar 大鼠（200-250 克，8 周大）在 PTZ（70 毫克/千克，IP）治疗前 30 分钟腹腔注射（IP）不同剂量的 ALP（15 和 30 毫克/千克）或载体。采用拉辛量表评估行为性癫痫发作。结果表明，根据 Racine 量表，ALP 的预处理降低了癫痫发作的阶段，显著延长了全身强直-阵挛发作（GTCS）的持续时间，并减少了肌阵挛抽搐的次数（P < 0.05）。总之，研究结果表明，PI3K 拮抗剂 ALP 通过 ALP 抑制 PI3K 信号通路，从而抑制 PTZ 诱导的癫痫发作。
{"title":"Effect of alpelisib, a selective phosphatidylinositol-3-kinase inhibitor, on seizure development in a rat pentylenetetrazole model","authors":"A. Rostamkhani, N. Mirazi, A. Hosseini","doi":"10.12681/jhvms.31037","DOIUrl":"https://doi.org/10.12681/jhvms.31037","url":null,"abstract":"Epilepsy is a neurological disease that results from an abnormality in the brain's activity. Phosphatidylinositol-3-kinase (PI3K) signaling pathway has played a crucial role in epilepsy pathogenesis. Alpelisib (ALP) is a selective inhibitor of PI3K. We examined the ability of ALP to treat pentylenetetrazole (PTZ)-induced convulsions in a rat model. Male Wistar rats (200-250 g, 8 weeks old) were injected intraperitoneally (IP) with ALP at different doses of 15 and 30 mg/kg, or vehicle 30 min prior to PTZ (70 mg/kg, IP) treatment. Racine's scale was used to assess behavioral seizures. The results showed that pretreatment with ALP decreased the seizure stages according to the Racine scale, significantly prolonged the duration of general tonic-clonic seizure (GTCS) and reduced the number of myoclonic jerks (P < 0.05). In conclusion, based on results it was shown that PI3K antagonist ALP inhibited PTZ-induced seizure by inhibiting the PI3K signaling pathway via ALP.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The feline herpesvirus (FHV) is a widely diffused and highly contagious virus that represents a common health problem in cats. It is frequently associated with diseases of different pathogenicity that can be particularly severe in young kittens determining viral pneumonia and sometimes, death. Unfortunately, there isn’t a well-defined therapeutic protocol against the virus. Several studies concerning the application of alternative treatments against herpesviruses have been performed with promising results, in both human and veterinary medicine. The present study aims to investigate in vitro antiviral effects against FHV of the commercially compound HELP-TH1 (Camon, S.p.A., Italy) mainly composed by Ganoderma lucidum, Cordyceps sinensis, and Trametes versicolor. The antiviral effect of HELP-TH1 was evaluated by the Plaque Reduction Assay and by the real time PCR quantifying and comparing the viral load of infected controls vs treated experimental samples. Our data indicate that HELP-TH1 inhibits the cytopathic effect of the virus and reduces the viral load in the experimental conditions demonstrating its antiviral effect.
{"title":"In vitro evaluation of a natural food supplement as inhibitors of feline herpesvirus replication","authors":"MI Pacini, P. Bonaccini, M. Mazzei, M. Forzan","doi":"10.12681/jhvms.30136","DOIUrl":"https://doi.org/10.12681/jhvms.30136","url":null,"abstract":"The feline herpesvirus (FHV) is a widely diffused and highly contagious virus that represents a common health problem in cats. It is frequently associated with diseases of different pathogenicity that can be particularly severe in young kittens determining viral pneumonia and sometimes, death. Unfortunately, there isn’t a well-defined therapeutic protocol against the virus. Several studies concerning the application of alternative treatments against herpesviruses have been performed with promising results, in both human and veterinary medicine. The present study aims to investigate in vitro antiviral effects against FHV of the commercially compound HELP-TH1 (Camon, S.p.A., Italy) mainly composed by Ganoderma lucidum, Cordyceps sinensis, and Trametes versicolor. The antiviral effect of HELP-TH1 was evaluated by the Plaque Reduction Assay and by the real time PCR quantifying and comparing the viral load of infected controls vs treated experimental samples. Our data indicate that HELP-TH1 inhibits the cytopathic effect of the virus and reduces the viral load in the experimental conditions demonstrating its antiviral effect.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139627240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hamzehali Tehrani, B. Shemshadi, P. Shayan, S. Shirali, N. Panahi
Abstract In many countries, small ruminants provide the primary source of human protein and play an important role in livestock production. Parasitic infections, especially gastrointestinal parasites, cause economic losses such as reduced fertility and milk production, damage to the leather industry, weight loss, treatment costs, and losses in severe infections. Thus, identifying and classifying these parasites, including the above cases, is essential for scientific research in any country. This study aimed to Prevalence of Abomasum Nematode infection in Sheep from North of Iran. In the present cross-sectional descriptive study, we have studied the prevalence of infection with abomasum nematodes in sheep in northern Iran from April 2020 to September 2021 in 2400 abomasum sheep slaughtered in industrial slaughterhouses in Gilan and Mazandaran provinces (1200 sheep from each province). With three age groups of below two years, two to four years, and above four years and gender segregation of hosts, after necropsy and preparation of sheep abomasal mucosa chip and isolation and segregation of samples, we studied the morphologic and morphometric characteristics of the samples. The actions were performed according to the taxonomic keys and using SPSS software V.16 and Paired Sample T-Test statistical test to determine the level of significance of the data (p <0.05) to classify and compare the obtained data and examine the ratio. The sex of the host was determined by the age of the animal and the prevalence of the parasite at the mentioned ages. The present study results and comparison of the prevalence of infection in Gilan and Mazandaran provinces showed that Marshallagia marshalli had the highest prevalence of 94.6% and 91.9% in Mazandaran and Gilan province, respectively. The prevalence of Ostertagia circumcincta was 87.2% and 77.2% in Gilan and Mazandaran provinces. The prevalence of Ostertagia occidentalis was 86.9% in Gilan province and 78% in Mazandaran province, while the prevalence of Parabronema skrjabini was 86.6% in Gilan province and 61.2% in Mazandaran province. The prevalence of Haemonchus contortus was 66.8% and 60.5% in Gilan and Mazandaran, the lowest prevalence in these two provinces. The present study results showed the importance of combating the spread of parasitic infections with strategic treatment methods and biological control of these parasites. Also, various reports of drug resistance in abomasal nematodes should be emphasized more and more by competent bodies. Keywords: Abomasum, Nematodes, North of Iran, Parasitic infections, Sheep.
{"title":"Prevalence of Abomasum Nematode infection in Sheep from North of Iran","authors":"M. Hamzehali Tehrani, B. Shemshadi, P. Shayan, S. Shirali, N. Panahi","doi":"10.12681/jhvms.29841","DOIUrl":"https://doi.org/10.12681/jhvms.29841","url":null,"abstract":"Abstract \u0000In many countries, small ruminants provide the primary source of human protein and play an important role in livestock production. Parasitic infections, especially gastrointestinal parasites, cause economic losses such as reduced fertility and milk production, damage to the leather industry, weight loss, treatment costs, and losses in severe infections. Thus, identifying and classifying these parasites, including the above cases, is essential for scientific research in any country. This study aimed to Prevalence of Abomasum Nematode infection in Sheep from North of Iran. In the present cross-sectional descriptive study, we have studied the prevalence of infection with abomasum nematodes in sheep in northern Iran from April 2020 to September 2021 in 2400 abomasum sheep slaughtered in industrial slaughterhouses in Gilan and Mazandaran provinces (1200 sheep from each province). With three age groups of below two years, two to four years, and above four years and gender segregation of hosts, after necropsy and preparation of sheep abomasal mucosa chip and isolation and segregation of samples, we studied the morphologic and morphometric characteristics of the samples. The actions were performed according to the taxonomic keys and using SPSS software V.16 and Paired Sample T-Test statistical test to determine the level of significance of the data (p <0.05) to classify and compare the obtained data and examine the ratio. The sex of the host was determined by the age of the animal and the prevalence of the parasite at the mentioned ages. The present study results and comparison of the prevalence of infection in Gilan and Mazandaran provinces showed that Marshallagia marshalli had the highest prevalence of 94.6% and 91.9% in Mazandaran and Gilan province, respectively. The prevalence of Ostertagia circumcincta was 87.2% and 77.2% in Gilan and Mazandaran provinces. The prevalence of Ostertagia occidentalis was 86.9% in Gilan province and 78% in Mazandaran province, while the prevalence of Parabronema skrjabini was 86.6% in Gilan province and 61.2% in Mazandaran province. The prevalence of Haemonchus contortus was 66.8% and 60.5% in Gilan and Mazandaran, the lowest prevalence in these two provinces. The present study results showed the importance of combating the spread of parasitic infections with strategic treatment methods and biological control of these parasites. Also, various reports of drug resistance in abomasal nematodes should be emphasized more and more by competent bodies. \u0000Keywords: Abomasum, Nematodes, North of Iran, Parasitic infections, Sheep.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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