Abstract: This paper presents the first study conducted in Algeria to determine the pre and post-race hematological and biochemical parameters of horses of a 90 km endurance race for eliminated horses’ category and those who completed the races with good performance. This study was conducted on 15 endurance horses (10 Arabian Thoroughbred, 4 Arabian-Barb and 1 Barb). Nine (9) horses successfully completed the endurance race, whereas the other 6 were eliminated. Blood samples were collected from each animal before and after the end of the race. Whole blood samples were used for measuring the blood-biochemical parameters. Our results showed a significant increase in the hematological parameters (RBC, HB, Ht and MCV), the total leucocyte count, neutrophils and platelets for the group of horses who finished the race successfully. A significant decrease was noticed for the lymphocytes and eosinophils numbers while serum biochemical parameters showed a significant increase of Ca, TG, urea, U-A, AST and CK and a decrease of Mg, CHE, GLU and ƔGT. This study showed that, for the eliminated horses, there were significant changes in values of the total leucocytic count, lymphocytes, neutrophils, HB and Ht as compared to good performance horses. Serum biochemical parameters showed a significant increase of TG, U-A, AST and CK and a significant decrease of Ca, Mg, CHE and ƔGT. Most of the hemato-biochemical parameters of both the eliminated horses and those who completed the race successfully depicted significant differences. The results of this study suggested that biochemical findings could help identify horses with an increased risk of developing metabolic alterations before their elimination during competitive endurance races of different distances. Therefore, proper management protocols, standard feeding and watering with proper preparation of animals before the race could prevent electrolytes imbalance and pathological affections during racing.
{"title":"Pre- and post -exercise variation of blood parameters on performance endurance horses: a first race study from Algeria","authors":"M. Chikhaoui, S. Fadhéla, Cza Belalia, N. Ayad","doi":"10.12681/jhvms.28752","DOIUrl":"https://doi.org/10.12681/jhvms.28752","url":null,"abstract":"Abstract: This paper presents the first study conducted in Algeria to determine the pre and post-race hematological and biochemical parameters of horses of a 90 km endurance race for eliminated horses’ category and those who completed the races with good performance. This study was conducted on 15 endurance horses (10 Arabian Thoroughbred, 4 Arabian-Barb and 1 Barb). Nine (9) horses successfully completed the endurance race, whereas the other 6 were eliminated. Blood samples were collected from each animal before and after the end of the race. Whole blood samples were used for measuring the blood-biochemical parameters. Our results showed a significant increase in the hematological parameters (RBC, HB, Ht and MCV), the total leucocyte count, neutrophils and platelets for the group of horses who finished the race successfully. A significant decrease was noticed for the lymphocytes and eosinophils numbers while serum biochemical parameters showed a significant increase of Ca, TG, urea, U-A, AST and CK and a decrease of Mg, CHE, GLU and ƔGT. This study showed that, for the eliminated horses, there were significant changes in values of the total leucocytic count, lymphocytes, neutrophils, HB and Ht as compared to good performance horses. Serum biochemical parameters showed a significant increase of TG, U-A, AST and CK and a significant decrease of Ca, Mg, CHE and ƔGT. Most of the hemato-biochemical parameters of both the eliminated horses and those who completed the race successfully depicted significant differences. The results of this study suggested that biochemical findings could help identify horses with an increased risk of developing metabolic alterations before their elimination during competitive endurance races of different distances. Therefore, proper management protocols, standard feeding and watering with proper preparation of animals before the race could prevent electrolytes imbalance and pathological affections during racing.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46659786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A case of prepubic tendon rupture in a Romanov ewe in which four live lambs were removed by cesarean section is presented. A three-year-old ewe, on the 142±3 days of the second gestation was brought to the Siirt University Animal Health Application and Research Center with a sudden downward expansion of the abdomen during the last one day. When ewe was examined reluctance to locomotion, mild depression, tachycardia, tachypnea and abdominal peculiar enlargement on the left side more than right side were noticed. Because of this case was detected early the general condition was not so bad and clinical examination revealed that swelling was non-painful and non-edematous. Ultrasonography was performed to detect viability of the fetuses, and to eliminate possibility of hernia of the abdominal muscles. Based on anamnesis, abdominal palpation, and clinical examination, this case was diagnosed with prepubic tendon rupture. The prognosis of saving the lives of ewe and fetuses depends on successfully handling all aspects of the case in the last period of pregnancy. Although it is not possible to repair the abdominal integrity surgically in cases of prepubic tendon rupture in the ewe, a planned cesarean operation is indicated to increase the survival rates of both ewe and lambs.
{"title":"Rupture of the Prepubic Tendon in a Quadruplet Pregnant Romanov Ewe","authors":"Ö. Yılmaz, Ö. Biçici, E. Karakaya-Bilen","doi":"10.12681/jhvms.28261","DOIUrl":"https://doi.org/10.12681/jhvms.28261","url":null,"abstract":" A case of prepubic tendon rupture in a Romanov ewe in which four live lambs were removed by cesarean section is presented. A three-year-old ewe, on the 142±3 days of the second gestation was brought to the Siirt University Animal Health Application and Research Center with a sudden downward expansion of the abdomen during the last one day. When ewe was examined reluctance to locomotion, mild depression, tachycardia, tachypnea and abdominal peculiar enlargement on the left side more than right side were noticed. Because of this case was detected early the general condition was not so bad and clinical examination revealed that swelling was non-painful and non-edematous. Ultrasonography was performed to detect viability of the fetuses, and to eliminate possibility of hernia of the abdominal muscles. Based on anamnesis, abdominal palpation, and clinical examination, this case was diagnosed with prepubic tendon rupture. The prognosis of saving the lives of ewe and fetuses depends on successfully handling all aspects of the case in the last period of pregnancy. Although it is not possible to repair the abdominal integrity surgically in cases of prepubic tendon rupture in the ewe, a planned cesarean operation is indicated to increase the survival rates of both ewe and lambs. ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48655137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to determine the direct and indirect effects of the independent variables presented by the lactation lenght (LL), age of calving (CAGE), and daily milk yield (DMY) on the dependent variable of lactation milk yield (LMY) in Anatolian buffaloes. In this study, a total of 3761 LMY records of the 834 Anatolian buffaloes calving between 2012 and 2017 in Tokat province and around were used as the research material. In the study, the simple correlation coefficients between the dependent variable of LMY and independent variables were determined positive and significant (P<0.001). The research specified the direct determination coefficients of the independent variables of the LL, CAGE, and DMY on the dependent variable of LMY as 0.378, 0.004, and 0.350, respectively. The path coefficients related to LL, CAGE, and DMY were determined as 0.615, 0.021, and 0.592, respectively. Since the DMY and LL examined in the study had a high direct impact on the lactation milk yield, therefore it could be suggested that using these data as criteria in breeding programs conducted in the herds evaluated in the study will increase the success in the selections applied.
{"title":"Determination of Direct and Indirect Effects on Milk Yield of Anatolian Buffaloes Using Path Analysis","authors":"A. Şahin, Y. Aksoy, A. Yildirim, Z. Ulutaş","doi":"10.12681/jhvms.28470","DOIUrl":"https://doi.org/10.12681/jhvms.28470","url":null,"abstract":"This study aimed to determine the direct and indirect effects of the independent variables presented by the lactation lenght (LL), age of calving (CAGE), and daily milk yield (DMY) on the dependent variable of lactation milk yield (LMY) in Anatolian buffaloes. In this study, a total of 3761 LMY records of the 834 Anatolian buffaloes calving between 2012 and 2017 in Tokat province and around were used as the research material. In the study, the simple correlation coefficients between the dependent variable of LMY and independent variables were determined positive and significant (P<0.001). The research specified the direct determination coefficients of the independent variables of the LL, CAGE, and DMY on the dependent variable of LMY as 0.378, 0.004, and 0.350, respectively. The path coefficients related to LL, CAGE, and DMY were determined as 0.615, 0.021, and 0.592, respectively. Since the DMY and LL examined in the study had a high direct impact on the lactation milk yield, therefore it could be suggested that using these data as criteria in breeding programs conducted in the herds evaluated in the study will increase the success in the selections applied.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48543576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the mixtures obtained by mixing quinoa flour with wheat flour in different proportions were added to chicken meat patties and their effects on some quality characteristics were investigated. The yields of the meatballs prepared with the mixes containing 50% and 100% quinoa flour were higher than those of other meatballs (69.59% and 69.71%, respectively). The moisture retention of the fried meatballs prepared with mixtures containing 50%, 70% and 100% quinoa flour was found to be 45.80%, 45.97% and 51.09%, respectively. The results indicated that the moisture retention of these meatballs was higher than those of meatballs containing 30 and 0% quinoa flour. In contrast, oil absorption rates in the fried samples were in the range of 4.46-5.65% for all quinoa-containing samples and were lower compared to the control sample. Firmness decreased in meat patties prepared with mixtures containing high quinoa rates. It was observed that quinoa flour did not have a negative effect on quality factors. It was concluded that especially the mixtures containing 30% and 50% quinoa flour can be recommended.
{"title":"The possibilities of using quinoa flour in the production of chicken meat patties","authors":"R. Meral, N. Kutlu, A. Alav, O. Kılınççeker","doi":"10.12681/jhvms.29784","DOIUrl":"https://doi.org/10.12681/jhvms.29784","url":null,"abstract":"In this study, the mixtures obtained by mixing quinoa flour with wheat flour in different proportions were added to chicken meat patties and their effects on some quality characteristics were investigated. The yields of the meatballs prepared with the mixes containing 50% and 100% quinoa flour were higher than those of other meatballs (69.59% and 69.71%, respectively). The moisture retention of the fried meatballs prepared with mixtures containing 50%, 70% and 100% quinoa flour was found to be 45.80%, 45.97% and 51.09%, respectively. The results indicated that the moisture retention of these meatballs was higher than those of meatballs containing 30 and 0% quinoa flour. In contrast, oil absorption rates in the fried samples were in the range of 4.46-5.65% for all quinoa-containing samples and were lower compared to the control sample. Firmness decreased in meat patties prepared with mixtures containing high quinoa rates. It was observed that quinoa flour did not have a negative effect on quality factors. It was concluded that especially the mixtures containing 30% and 50% quinoa flour can be recommended.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46050772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In vitro antimicrobial and antibiofilm forming activities of Origanum munzurense extract were investigated against some gram-positive bacteria and yeast strains. Liquid microdilution method and microplate method were used for antimicrobial and antibiofilm forming activities, respectively. Minimal inhibitory concentration values were found to be 2 mg/ml for Enterococcus faecalis ATCC 29212, 64 mg/ml for Listeria monocytogenes RSKK 02028, 2 mg/ml for L. monocytogenes RSKK 472, 2 mg/ml for Bacillus cereus NRRL 569 , 128 mg/ml for S. aureus ATCC 25923, 32 mg/ml for S. aureus (MRSA) ATCC 43300 strain, 64 mg/ml for Candida tropicalis ATCC 13803, 2 mg/ml for C. albicans ATCC 90028, 1 mg/ml for S. aureus FRI 918 and 1 mg/ml for Staphylococcus epidermidis ATCC 12228 strain. The extract prevented the biofilm formations of E. faecalis ATCC 29212, S. aureus FRI 918 and S.epidermidis ATCC 12228 at concentrations of 2 mg/ml, 500 µg/ml and 250 µg/ml, respectively. No biofilm formation was observed for the other bacteria tested.As a result, Origanum munzurense extract has antibacterial and antibiofilm activity on the selected pathogenic microorganisms tested. Due to these properties of O. munzurense, it could be considered as a plant-based antimicrobial agent to be used especially in the food industry.
{"title":"Antimicrobial and antibiofilm forming activity of Origanum munzurense against some Gram-Positive bacteria and yeast","authors":"E. Zeybek, A. Kart, H. Yalçin","doi":"10.12681/jhvms.28910","DOIUrl":"https://doi.org/10.12681/jhvms.28910","url":null,"abstract":"In vitro antimicrobial and antibiofilm forming activities of Origanum munzurense extract were investigated against some gram-positive bacteria and yeast strains. Liquid microdilution method and microplate method were used for antimicrobial and antibiofilm forming activities, respectively. Minimal inhibitory concentration values were found to be 2 mg/ml for Enterococcus faecalis ATCC 29212, 64 mg/ml for Listeria monocytogenes RSKK 02028, 2 mg/ml for L. monocytogenes RSKK 472, 2 mg/ml for Bacillus cereus NRRL 569 , 128 mg/ml for S. aureus ATCC 25923, 32 mg/ml for S. aureus (MRSA) ATCC 43300 strain, 64 mg/ml for Candida tropicalis ATCC 13803, 2 mg/ml for C. albicans ATCC 90028, 1 mg/ml for S. aureus FRI 918 and 1 mg/ml for Staphylococcus epidermidis ATCC 12228 strain. The extract prevented the biofilm formations of E. faecalis ATCC 29212, S. aureus FRI 918 and S.epidermidis ATCC 12228 at concentrations of 2 mg/ml, 500 µg/ml and 250 µg/ml, respectively. No biofilm formation was observed for the other bacteria tested.As a result, Origanum munzurense extract has antibacterial and antibiofilm activity on the selected pathogenic microorganisms tested. Due to these properties of O. munzurense, it could be considered as a plant-based antimicrobial agent to be used especially in the food industry.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41401761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study describes a case of giant cell osteosarcoma (GCO) localised on the right scapulae in a six-year-old neutered male Scottish Fold cat. GCO is a rare tumour in domestic animals. This tumour is also known as giant cell-rich osteosarcoma. Multinucleated giant cells and osteoid structures are observed in GCO. The mass was 4.6 x 4.4 x 4.3 cm in size and very firm. Microscopically, fusiform spindle-shaped cells and multinucleated giant cells were observed. There were 4-8 mitotic figures in three random high-power fields (400x). Osteoid structures and necrosis were detected in several areas. In these areas, atypia was observed in the cells. Immunohistochemically, vimentin expression was observed in neoplastic cells and multinucleated giant cells. No immunoreactivity against actin and cytokeratin was observed in neoplastic cells. Additionally, vimentin expression was detected in the periphery of blood vessels and actin expression in blood vessel smooth muscle cells. As a result of histopathological and immunohistochemical findings, the mass was determined to be GCO.
本研究描述了一例6岁的雄性苏格兰Fold猫右肩胛骨巨细胞骨肉瘤（GCO）。GCO是一种罕见的家畜肿瘤。这种肿瘤也被称为富含巨细胞的骨肉瘤。在GCO中观察到多核巨细胞和类骨结构。质量为4.6 x 4.4 x 4.3厘米，非常坚固。显微镜下观察到梭形纺锤形细胞和多核巨细胞。在三个随机的高功率场（400x）中有4-8个有丝分裂像。在几个区域检测到骨样结构和坏死。在这些区域，在细胞中观察到异型性。免疫组化观察到肿瘤细胞和多核巨细胞中波形蛋白的表达。在肿瘤细胞中未观察到针对肌动蛋白和细胞角蛋白的免疫反应性。此外，在血管周围检测到波形蛋白的表达，在血管平滑肌细胞中检测到肌动蛋白的表达。根据组织病理学和免疫组织化学结果，该肿块被确定为GCO。
{"title":"A case of giant cell osteosarcoma in a Scottish fold cat","authors":"B. Dayı, AM Mutlu, A. Akkoç, A. Sevimli","doi":"10.12681/jhvms.28130","DOIUrl":"https://doi.org/10.12681/jhvms.28130","url":null,"abstract":"This study describes a case of giant cell osteosarcoma (GCO) localised on the right scapulae in a six-year-old neutered male Scottish Fold cat. GCO is a rare tumour in domestic animals. This tumour is also known as giant cell-rich osteosarcoma. Multinucleated giant cells and osteoid structures are observed in GCO. The mass was 4.6 x 4.4 x 4.3 cm in size and very firm. Microscopically, fusiform spindle-shaped cells and multinucleated giant cells were observed. There were 4-8 mitotic figures in three random high-power fields (400x). Osteoid structures and necrosis were detected in several areas. In these areas, atypia was observed in the cells. Immunohistochemically, vimentin expression was observed in neoplastic cells and multinucleated giant cells. No immunoreactivity against actin and cytokeratin was observed in neoplastic cells. Additionally, vimentin expression was detected in the periphery of blood vessels and actin expression in blood vessel smooth muscle cells. As a result of histopathological and immunohistochemical findings, the mass was determined to be GCO.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44653505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Titaouine, F. Mezerdi, A. Makhlouf, N. Mohamdi, H. Mohamdi
ABSTRACTThis study aims to evaluate the effect of body condition score (BCS) on hormonal profiles and to reveal their influence on the blood metabolites in Ouled Djellal ewes during the mating period. The experiment was conducted on thirty, clinically healthy and non-pregnant, Ouled Djellal ewes which have been kept into two groups according to their body condition score (Group I: BCS < 2.50 units; n = 13, Group II: BCS > 2.50 units; n = 17). In both groups, oestrus was synchronized using hormonal patterns then the ewes were left to be naturally inseminated. Blood samples were collected at the time of the sponge removal. Plasma concentrations of glucose, triglycerides, cholesterol, total protein, albumin, creatinine, urea, triiodothyronine, thyroxine and Progesterone were recorded. The plasma level of triglycerides and thyroid hormones (T3 and T4) increase significantly (P < 0.01) in group II. Progesterone concentrations were similar (P ≥ 0.05) in both groups at mating period, although we recorded higher rate in group I. Therefore, we conclude that body condition score has a significant impact on some hormone profiles and blood parameters during the mating period.
{"title":"Effect of body condition score on biochemical parameters and hormonal profiles during the mating period in Ouled Djellal ewes in arid zone","authors":"M. Titaouine, F. Mezerdi, A. Makhlouf, N. Mohamdi, H. Mohamdi","doi":"10.12681/jhvms.29043","DOIUrl":"https://doi.org/10.12681/jhvms.29043","url":null,"abstract":"ABSTRACTThis study aims to evaluate the effect of body condition score (BCS) on hormonal profiles and to reveal their influence on the blood metabolites in Ouled Djellal ewes during the mating period. The experiment was conducted on thirty, clinically healthy and non-pregnant, Ouled Djellal ewes which have been kept into two groups according to their body condition score (Group I: BCS < 2.50 units; n = 13, Group II: BCS > 2.50 units; n = 17). In both groups, oestrus was synchronized using hormonal patterns then the ewes were left to be naturally inseminated. Blood samples were collected at the time of the sponge removal. Plasma concentrations of glucose, triglycerides, cholesterol, total protein, albumin, creatinine, urea, triiodothyronine, thyroxine and Progesterone were recorded. The plasma level of triglycerides and thyroid hormones (T3 and T4) increase significantly (P < 0.01) in group II. Progesterone concentrations were similar (P ≥ 0.05) in both groups at mating period, although we recorded higher rate in group I. Therefore, we conclude that body condition score has a significant impact on some hormone profiles and blood parameters during the mating period. ","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43893141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACTThere are intercellular communication and social behaviors, including Quorum Sensing System (QS) in bacteria. The present study aims to investigate the production of QS signal molecules (N-acyl homoserine lactones, AHLs) in bacterial fish pathogen Vibrio alginolyticus (3 strains) and also determine the virulence using these AHLs under the management of the QS in V. alginolyticus. QS of V. alginolyticus was proved by cross validation assay used by AHL marker bacteria strains (Agrobacterium tumefaciens NT1 and Chromobacterium violaceum CV026). V. alginolyticus was not produced N-butanoyl homoserine lactone (BHL), while N-(3-okzododekanoyl)-L-homoserine lactone (OdDHL) production was detected in V. alginolyticus. The presence of virulence factors, such as biofilm, pigment, rhamnolipid, hemolysis, elastase, protease and amylase production, was investigated as phenotypically in V. alginolyticus. V. alginolyticus was found to produce biofilm, pigment, amylase and elastase; however, it was not determined to produce rhamnolipid, hemolysis and protease virulence.
{"title":"Detection of Quorum Sensing System (Cell to Cell Communication) Using Marker Strains in Vibrio alginolyticus Strains and Determine Virulence under Master of this System","authors":"N. Filik, A. Kubilay","doi":"10.12681/jhvms.28525","DOIUrl":"https://doi.org/10.12681/jhvms.28525","url":null,"abstract":"ABSTRACTThere are intercellular communication and social behaviors, including Quorum Sensing System (QS) in bacteria. The present study aims to investigate the production of QS signal molecules (N-acyl homoserine lactones, AHLs) in bacterial fish pathogen Vibrio alginolyticus (3 strains) and also determine the virulence using these AHLs under the management of the QS in V. alginolyticus. QS of V. alginolyticus was proved by cross validation assay used by AHL marker bacteria strains (Agrobacterium tumefaciens NT1 and Chromobacterium violaceum CV026). V. alginolyticus was not produced N-butanoyl homoserine lactone (BHL), while N-(3-okzododekanoyl)-L-homoserine lactone (OdDHL) production was detected in V. alginolyticus. The presence of virulence factors, such as biofilm, pigment, rhamnolipid, hemolysis, elastase, protease and amylase production, was investigated as phenotypically in V. alginolyticus. V. alginolyticus was found to produce biofilm, pigment, amylase and elastase; however, it was not determined to produce rhamnolipid, hemolysis and protease virulence.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45466180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small ruminant lentiviruses (SRLVs) are viral pathogens that are common in goats and sheep, affect production, and cause significant economic losses in small ruminant breeding. Caprine arthritis encephalitis virus (CAEV) and Maedi-Visna virus (MVV) are prototypes of SRLVs. Both of them affect animal health and welfare in sheep and goats and cause progressive and persistent infections in the small ruminant industry. The present study aimed to reveal the epidemiological status of lentivirus infection in the sheep region in Yalova province located in the southeast part of the Marmara region and determine the circulating genotypes by conducting the sequence analysis of the samples detected positive by a molecular method and molecular characterization of the detected field strains. To that end, 231 sheep blood samples were used between May 2016 and April 2018. Based on sampling results of the PCR test and ELISA tests, 5.62% (13/231) and 5.19% (12/231) positivity rates were found in sheep, respectively. According to the ELISA test results, a significant difference was found in terms of age groups (6 months -1 age, 1-3 age, >3 age) (χ2: 6.01; p=0.04). Furthermore, the sequence analysis of the gag gene region detected the existence of the A genotype of small ruminant lentiviruses in sheep. The data obtained from the study revealed a low seroprevalence course of SRLV infection in the study area in the absence of a systematic disease control program.
{"title":"Investigation of the Epidemiology of Small Ruminant Lentivirus Infections southeast part of the Marmara region of Turkey","authors":"A. Sait, OB Ince","doi":"10.12681/jhvms.29095","DOIUrl":"https://doi.org/10.12681/jhvms.29095","url":null,"abstract":"Small ruminant lentiviruses (SRLVs) are viral pathogens that are common in goats and sheep, affect production, and cause significant economic losses in small ruminant breeding. Caprine arthritis encephalitis virus (CAEV) and Maedi-Visna virus (MVV) are prototypes of SRLVs. Both of them affect animal health and welfare in sheep and goats and cause progressive and persistent infections in the small ruminant industry. The present study aimed to reveal the epidemiological status of lentivirus infection in the sheep region in Yalova province located in the southeast part of the Marmara region and determine the circulating genotypes by conducting the sequence analysis of the samples detected positive by a molecular method and molecular characterization of the detected field strains. To that end, 231 sheep blood samples were used between May 2016 and April 2018. Based on sampling results of the PCR test and ELISA tests, 5.62% (13/231) and 5.19% (12/231) positivity rates were found in sheep, respectively. According to the ELISA test results, a significant difference was found in terms of age groups (6 months -1 age, 1-3 age, >3 age) (χ2: 6.01; p=0.04). Furthermore, the sequence analysis of the gag gene region detected the existence of the A genotype of small ruminant lentiviruses in sheep. The data obtained from the study revealed a low seroprevalence course of SRLV infection in the study area in the absence of a systematic disease control program.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43094881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BZ Milovanović, MM Đorđević, BM Savić, NV Jezdimirović, NM Zdravković, Z. Zurovac-Sapundzic, D. Glišić, M. Ninković, B. Kureljušić
A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of the Institute of Veterinary Medicine of Serbia for determining the potential cause of death. Necropsy revealed massive hemorrhages in subcutaneous and intermuscular tissue and on papillary muscle. Petechiae and ecchymoses were found on the omentum, mesentery and adipose tissue of heart, kidney and costal pleura. Haemorrhagic-necrotic enteritis in duodenum and jejunum was characterized by catarrhal hemorrhagic inflammation with the presence of mucous and bloody content, whereas gas bubbles in the submucosa have also been confirmed. Bacterial cultures from sampled organs were identified as Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli. Based on the established pathological and histological changes and the results of the bacteriological, biochemical, and molecular examination, the state of septic shock and toxemia with disseminated massive bleeding was the immediate cause of mouflon death. The septic condition is a consequence of enterotoxemia caused by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli infection.
{"title":"Concurrent infection by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli in mouflon (Ovis aries musimon) - A case report","authors":"BZ Milovanović, MM Đorđević, BM Savić, NV Jezdimirović, NM Zdravković, Z. Zurovac-Sapundzic, D. Glišić, M. Ninković, B. Kureljušić","doi":"10.12681/jhvms.28086","DOIUrl":"https://doi.org/10.12681/jhvms.28086","url":null,"abstract":"A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of the Institute of Veterinary Medicine of Serbia for determining the potential cause of death. Necropsy revealed massive hemorrhages in subcutaneous and intermuscular tissue and on papillary muscle. Petechiae and ecchymoses were found on the omentum, mesentery and adipose tissue of heart, kidney and costal pleura. Haemorrhagic-necrotic enteritis in duodenum and jejunum was characterized by catarrhal hemorrhagic inflammation with the presence of mucous and bloody content, whereas gas bubbles in the submucosa have also been confirmed. Bacterial cultures from sampled organs were identified as Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli. Based on the established pathological and histological changes and the results of the bacteriological, biochemical, and molecular examination, the state of septic shock and toxemia with disseminated massive bleeding was the immediate cause of mouflon death. The septic condition is a consequence of enterotoxemia caused by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli infection.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48782139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：