Linguatuliasis is caused by the Arthropod parasite Linguatula serrata (family: Linguatulidae) a parasite with worldwide distribution and zoonotic importance, especially in warm subtropical and temperate regions. Dogs, cats, foxes, jackals, and other carnivores are the final hosts of this Arthropod while any mammal could be a potential intermediate host of this parasite. Adults L. serrata live in the nasopharyngeal region and lower respiratory system, embed their body into the mucosa of nasal pharyngeal cavities. A two year old spayed female dog of undetermined breed, weighting 29 kg that lives in a mountain village called Sella (Prefecture of Achaia, Greece) in a semi-free form, was presented in a veterinary clinic of Patra with history of progressive cough and sneezing, which started about 2 weeks earlier. Along with the dog, the owner brought a parasite that was expelled from the dog’s nose during the intense cough and sneezing the same day. Macroscopic morphological aspects of the parasite, in the laboratory under stereoscope, revealed an arthropod 3,8cm in length identified as L. serrata, also commonly called tongue worm. The dog was treated with Macrocyclice lactone and lavage of nasal cavities. Humans can become infected in two ways: either as an intermediate host (visceral linguatuliasis) or as an accidental final host (nasopharyngeal linguatuliasis), usually as a result of consumption of raw or poorly cooked meat of an infected intermediate host.
{"title":"Management of Linguatula serrata infestation in a dog from Greece: a case report","authors":"M Lefkaditis, I Zapantes, A Giannouli","doi":"10.12681/jhvms.30904","DOIUrl":"https://doi.org/10.12681/jhvms.30904","url":null,"abstract":"Linguatuliasis is caused by the Arthropod parasite Linguatula serrata (family: Linguatulidae) a parasite with worldwide distribution and zoonotic importance, especially in warm subtropical and temperate regions. Dogs, cats, foxes, jackals, and other carnivores are the final hosts of this Arthropod while any mammal could be a potential intermediate host of this parasite. Adults L. serrata live in the nasopharyngeal region and lower respiratory system, embed their body into the mucosa of nasal pharyngeal cavities. A two year old spayed female dog of undetermined breed, weighting 29 kg that lives in a mountain village called Sella (Prefecture of Achaia, Greece) in a semi-free form, was presented in a veterinary clinic of Patra with history of progressive cough and sneezing, which started about 2 weeks earlier. Along with the dog, the owner brought a parasite that was expelled from the dog’s nose during the intense cough and sneezing the same day. Macroscopic morphological aspects of the parasite, in the laboratory under stereoscope, revealed an arthropod 3,8cm in length identified as L. serrata, also commonly called tongue worm. The dog was treated with Macrocyclice lactone and lavage of nasal cavities. Humans can become infected in two ways: either as an intermediate host (visceral linguatuliasis) or as an accidental final host (nasopharyngeal linguatuliasis), usually as a result of consumption of raw or poorly cooked meat of an infected intermediate host.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TA Vijayalingam, NV Rajesh, S Vairamuthu, M Boopathy Raja, NK Sudeep Kumar
The present study was conducted to evaluate the dietary effects of two seaweeds (Sargassum wightii and Gracilaria corticata) on different physiological parameters of TANUVAS Aseel chicken. A total of 30 TANUVAS Aseel chicks, day old were allocated into three groups (Each with 10 chicks) viz., one control group (C1) and two treatment groups (T1 and T2). The control group (C1) were fed with commercial grower feed alone and the treatment group (T1) had control diet with 5% Sargassum wightii and the treatment group (T2) had control diet mixed with 5% Gracilaria corticata. Parameters were recorded for a continuous period of 16 weeks in 4 weeks interval. The growth performance was found to be non-significant (P ≥ 0.05) during 0 day of feed trials and highly significant (P ≤ 0.01) during 4th, 8th, 12th and 16th week of feed trails. Haematological parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference exist in lymphocyte, monocyte and eosinophil count and a highly significant (P ≤ 0.01) difference exist in platelets count between the control (C1) and treatment groups (T1 and T2). Serum biochemical parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference in Uric acid, creatinine, ALT, calcium and cholesterol level and a highly significant (P ≤ 0.01) difference in Total protein, albumin, AST, glucose, triglycerides, phosphorus, magnesium, potassium and chloride level between the control (C1) and treatment groups (T1 and T2). However non-significant (P ≥ 0.05) difference exists in globulin and sodium content only during 16th week of feed trials. It could be noted that 5% inclusion of red seaweed, Gracilaria corticata in commercial grower feed as a feed supplement to TANUVAS Aseel chicks had a better body weight gain than 5% inclusion level of brown seaweed, Sargassum wightii in commercial grower feed. Based on the haematological and serum biochemical analysis, the supplementation of 5% inclusion level of seaweeds in this trial did not pose any threat to the physiological well-being of TANUVAS Aseel chicken.
{"title":"Effect of dietary supplementation of seaweeds on growth and blood profiles of TANUVAS Aseel chicken","authors":"TA Vijayalingam, NV Rajesh, S Vairamuthu, M Boopathy Raja, NK Sudeep Kumar","doi":"10.12681/jhvms.30812","DOIUrl":"https://doi.org/10.12681/jhvms.30812","url":null,"abstract":"The present study was conducted to evaluate the dietary effects of two seaweeds (Sargassum wightii and Gracilaria corticata) on different physiological parameters of TANUVAS Aseel chicken. A total of 30 TANUVAS Aseel chicks, day old were allocated into three groups (Each with 10 chicks) viz., one control group (C1) and two treatment groups (T1 and T2). The control group (C1) were fed with commercial grower feed alone and the treatment group (T1) had control diet with 5% Sargassum wightii and the treatment group (T2) had control diet mixed with 5% Gracilaria corticata. Parameters were recorded for a continuous period of 16 weeks in 4 weeks interval. The growth performance was found to be non-significant (P ≥ 0.05) during 0 day of feed trials and highly significant (P ≤ 0.01) during 4th, 8th, 12th and 16th week of feed trails. Haematological parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference exist in lymphocyte, monocyte and eosinophil count and a highly significant (P ≤ 0.01) difference exist in platelets count between the control (C1) and treatment groups (T1 and T2). Serum biochemical parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference in Uric acid, creatinine, ALT, calcium and cholesterol level and a highly significant (P ≤ 0.01) difference in Total protein, albumin, AST, glucose, triglycerides, phosphorus, magnesium, potassium and chloride level between the control (C1) and treatment groups (T1 and T2). However non-significant (P ≥ 0.05) difference exists in globulin and sodium content only during 16th week of feed trials. It could be noted that 5% inclusion of red seaweed, Gracilaria corticata in commercial grower feed as a feed supplement to TANUVAS Aseel chicks had a better body weight gain than 5% inclusion level of brown seaweed, Sargassum wightii in commercial grower feed. Based on the haematological and serum biochemical analysis, the supplementation of 5% inclusion level of seaweeds in this trial did not pose any threat to the physiological well-being of TANUVAS Aseel chicken.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Type 1 diabetes (T1D) is a chronic autoimmune disease that even with insulin therapy, inflammatory complications will develop in the long term. 40 inbred C57BL/6 mice were randomly divided into four groups (n=10): Control group consisted of healthy mice receiving citrate buffer, Diabetic group included a group of diabetic mice, Diabetic+TX group was a group of diabetic mice treated with troxerutin (TX), and TX group was a group of healthy mice treated with TX. Two weeks after the final dose of streptozotocin (STZ), The cytokine levels were measured using ELISA in the culture supernatants of spleen cells after 72 hours. Radioimmunoassay was used to measure insulin and c-peptide levels. The fasting blood sugar (FBS) was measured by an automatic glucometer device. lymphocyte proliferation index was evaluated using MTT assay, myeloperoxidase (MPO) level was measured in serum and pathologic studies of the kidney and liver were performed. The levels of IL-1, IL-17, TNF-α and IFN-γ as well as MPO, FBS levels and proliferation index was significantly decreased in the treated diabetic group compared to the diabetic mice (p<0.05). plasma C-peptide and insulin significantly increased in treated diabetic group than in the diabetic mice (p<0.05). Histologically, in diabetic animals treated with Tx, inflammatory and degenerative processes in both kidney and liver tissues were alleviated significantly (p<0.05). According to the results, it was supported the anti-diabetic and anti-inflammatory effects of TX, however, more studies are needed to investigate the effects of TX and the dose-response relationship in this disease.
{"title":"Troxerutin as an anti-inflammatory and anti-oxidative drug could ameliorate Type 1 diabetes complications in C57BL/6 mice","authors":"Y Jafari-Khataylou, S Ahmadiafshar, N Mirzakhani","doi":"10.12681/jhvms.31426","DOIUrl":"https://doi.org/10.12681/jhvms.31426","url":null,"abstract":"Type 1 diabetes (T1D) is a chronic autoimmune disease that even with insulin therapy, inflammatory complications will develop in the long term. 40 inbred C57BL/6 mice were randomly divided into four groups (n=10): Control group consisted of healthy mice receiving citrate buffer, Diabetic group included a group of diabetic mice, Diabetic+TX group was a group of diabetic mice treated with troxerutin (TX), and TX group was a group of healthy mice treated with TX. Two weeks after the final dose of streptozotocin (STZ), The cytokine levels were measured using ELISA in the culture supernatants of spleen cells after 72 hours. Radioimmunoassay was used to measure insulin and c-peptide levels. The fasting blood sugar (FBS) was measured by an automatic glucometer device. lymphocyte proliferation index was evaluated using MTT assay, myeloperoxidase (MPO) level was measured in serum and pathologic studies of the kidney and liver were performed. The levels of IL-1, IL-17, TNF-α and IFN-γ as well as MPO, FBS levels and proliferation index was significantly decreased in the treated diabetic group compared to the diabetic mice (p<0.05). plasma C-peptide and insulin significantly increased in treated diabetic group than in the diabetic mice (p<0.05). Histologically, in diabetic animals treated with Tx, inflammatory and degenerative processes in both kidney and liver tissues were alleviated significantly (p<0.05). According to the results, it was supported the anti-diabetic and anti-inflammatory effects of TX, however, more studies are needed to investigate the effects of TX and the dose-response relationship in this disease.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AR Belmamoun, A Ammam, C Mhamdia, R Chadli, A Baki, N Madouni
Raw milk contamination by Methicillin-resistant S. aureus (MRSA) threatens food safety and leads to public health problems. Our study evaluates biofilm formation and virulence factors among 21 MRSA in raw cow’s milk. Methicillin resistance was confirmed by cefoxitin screening using the automated VITEK2 system, with a minimum inhibitory concentration greater than 8 mg/l. Qualitative characterization of biofilm-producing strains was performed on Congo red agar (CRA), with a semi-quantitative adhesion test on 96-well tissue culture plates (TCP). The ability to produce different enzymes was evaluated, such as caseinase, lipase, and phospholipase (lecithinase). The surface hydrophobicity of the bacteria was determined, and the autoaggregation test was used to predict the interactions between bacterial cells. Among the tested strains, 61.9% were biofilm producers in the CRA, developing a positive and variable phenotype. Furthermore, 19.05% and 80.95% of isolates were high and low biofilm formation on TCP. The enzymatic activity showed that lécithinase, caseinase, and lipase activities were detected in 100%, 80.95%, and 80.95% of cases. Highly hydrophilic (85.71%) and weakly hydrophobic (14.29%) were detected in MRSA isolates. The strains showed that 71.43% were moderate autoaggregation and 28.57% were low autoaggregation. No significant difference was found between the CRA method and TCP (P> 0.05). A significant association was found between adhesion capacity and bacterial autoaggregation in S. aureus strains (p>0.05). On the other hand, no statistical association between the hydrophobicity of microbial strains and adhesion capacity (p<0.05) was found. The same result was for the hydrophobicity of microbial strains and autoaggregation (p<0.05). This investigation could be beneficial for developing new control measures, prevention, and effective treatment against staphylococcal bacterial resistance.
{"title":"Presence of methicillin-resistant Staphylococcus aureus in raw cow’s milk: adhesive capacities and extracellular enzymes characterization","authors":"AR Belmamoun, A Ammam, C Mhamdia, R Chadli, A Baki, N Madouni","doi":"10.12681/jhvms.31440","DOIUrl":"https://doi.org/10.12681/jhvms.31440","url":null,"abstract":"Raw milk contamination by Methicillin-resistant S. aureus (MRSA) threatens food safety and leads to public health problems. Our study evaluates biofilm formation and virulence factors among 21 MRSA in raw cow’s milk. Methicillin resistance was confirmed by cefoxitin screening using the automated VITEK2 system, with a minimum inhibitory concentration greater than 8 mg/l. Qualitative characterization of biofilm-producing strains was performed on Congo red agar (CRA), with a semi-quantitative adhesion test on 96-well tissue culture plates (TCP). The ability to produce different enzymes was evaluated, such as caseinase, lipase, and phospholipase (lecithinase). The surface hydrophobicity of the bacteria was determined, and the autoaggregation test was used to predict the interactions between bacterial cells. Among the tested strains, 61.9% were biofilm producers in the CRA, developing a positive and variable phenotype. Furthermore, 19.05% and 80.95% of isolates were high and low biofilm formation on TCP. The enzymatic activity showed that lécithinase, caseinase, and lipase activities were detected in 100%, 80.95%, and 80.95% of cases. Highly hydrophilic (85.71%) and weakly hydrophobic (14.29%) were detected in MRSA isolates. The strains showed that 71.43% were moderate autoaggregation and 28.57% were low autoaggregation. No significant difference was found between the CRA method and TCP (P> 0.05). A significant association was found between adhesion capacity and bacterial autoaggregation in S. aureus strains (p>0.05). On the other hand, no statistical association between the hydrophobicity of microbial strains and adhesion capacity (p<0.05) was found. The same result was for the hydrophobicity of microbial strains and autoaggregation (p<0.05). This investigation could be beneficial for developing new control measures, prevention, and effective treatment against staphylococcal bacterial resistance.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Voulgarakis, D Gougoulis, D Psalla, G Papakonstantinou, M Angelidou-Tsifida, V Papatsiros, LV Athanasiou, G Christodoulopoulos
Abstract
Ruminal acidosis is presented as the most considerable nutritional disorder of ruminants with severe impacts in animal health, animal welfare and considerable economics losses in ovine and bovine herds. The disease can be distinguished as Acute (ARA) and Sub-Acute Ruminal Acidosis (SARA). SARA constitutes as the main nutritional disorder in intensive ruminant farming with several complications, such as liver abscesses, milk fat depression, reduced milk yield and early culling. This paper constitutes the first part of a thorough review about ruminal acidosis. The first part focuses on definition, types of ruminal acidosis, epidemiology, economic impacts and welfare implications.
{"title":"Ruminal Acidosis Part I: Clinical manifestations, epidemiology and impact of the disease","authors":"N Voulgarakis, D Gougoulis, D Psalla, G Papakonstantinou, M Angelidou-Tsifida, V Papatsiros, LV Athanasiou, G Christodoulopoulos","doi":"10.12681/jhvms.31237","DOIUrl":"https://doi.org/10.12681/jhvms.31237","url":null,"abstract":"Abstract
 Ruminal acidosis is presented as the most considerable nutritional disorder of ruminants with severe impacts in animal health, animal welfare and considerable economics losses in ovine and bovine herds. The disease can be distinguished as Acute (ARA) and Sub-Acute Ruminal Acidosis (SARA). SARA constitutes as the main nutritional disorder in intensive ruminant farming with several complications, such as liver abscesses, milk fat depression, reduced milk yield and early culling. This paper constitutes the first part of a thorough review about ruminal acidosis. The first part focuses on definition, types of ruminal acidosis, epidemiology, economic impacts and welfare implications.
","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"37 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to isolate the Enterococcus species from homemade water buffalo clotted cream sold in Afyonkarahisar, investigate the vancomycin resistance genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. A total of 107 buffalo clotted cream samples sold in public bazaars of Afyonkarahisar were collected. Following the phenotypic identification using a commercial identification kit, PCR was applied to strains using by species specific primers. Forty Enterococcus strains were obtained from 107 samples by PCR. Of 40 strains, 31 (77.5%) and 9 (22.5%) were identified to be E. faecalis and E. faecium, respectively. One vanA (2.5%) and 14 vanB (35%) genes were determined in tested 40 strains. While of strains having the vanB gen, 10 and four were found to be E. faecalis and E. faecium, respectively, one E. faecium strain also harboured the vanA gen. Thus, while the vanB gen positivity was found to be 32.3% in 31 E. faecalis strains and 44.4% in 9 E. faecium strains, the vanA gen positivity was determined to be 11.1% in 9 E. faecium strains. It was not found the vanC1 and vanC2/C3 genes in none of the strains. While all of strains were phenotypically resistant to streptomycin and fusidic acid, high resistance rates were also determined in the strains to kanamycin (87.5%), cephalothin (80%), erythromycin (80%), gentamicin (77.5%), tetracycline (75%) and vancomycin (60%). Resistance to all tested antibiotics except teicoplanin was determined in E. faecalis strains and except chloramphenicol in E. faecium strains harbouring the vancomycin resistance genes. In conclusion, it was thought the buffalo clotted creams offered for consumption in Afyonkarahisar may have potential risk for public health in terms of VRE species. To our knowledge, this is the first study showing the presence of VRE in the clotted cream samples by PCR in Turkey.
{"title":"First report of vancomycin resistant Enterococcus faecalis and Enterococcus faecium isolated from water buffalo clotted cream in Turkey","authors":"E Seker, YN Dogan","doi":"10.12681/jhvms.30877","DOIUrl":"https://doi.org/10.12681/jhvms.30877","url":null,"abstract":"This study aimed to isolate the Enterococcus species from homemade water buffalo clotted cream sold in Afyonkarahisar, investigate the vancomycin resistance genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. A total of 107 buffalo clotted cream samples sold in public bazaars of Afyonkarahisar were collected. Following the phenotypic identification using a commercial identification kit, PCR was applied to strains using by species specific primers. Forty Enterococcus strains were obtained from 107 samples by PCR. Of 40 strains, 31 (77.5%) and 9 (22.5%) were identified to be E. faecalis and E. faecium, respectively. One vanA (2.5%) and 14 vanB (35%) genes were determined in tested 40 strains. While of strains having the vanB gen, 10 and four were found to be E. faecalis and E. faecium, respectively, one E. faecium strain also harboured the vanA gen. Thus, while the vanB gen positivity was found to be 32.3% in 31 E. faecalis strains and 44.4% in 9 E. faecium strains, the vanA gen positivity was determined to be 11.1% in 9 E. faecium strains. It was not found the vanC1 and vanC2/C3 genes in none of the strains. While all of strains were phenotypically resistant to streptomycin and fusidic acid, high resistance rates were also determined in the strains to kanamycin (87.5%), cephalothin (80%), erythromycin (80%), gentamicin (77.5%), tetracycline (75%) and vancomycin (60%). Resistance to all tested antibiotics except teicoplanin was determined in E. faecalis strains and except chloramphenicol in E. faecium strains harbouring the vancomycin resistance genes. In conclusion, it was thought the buffalo clotted creams offered for consumption in Afyonkarahisar may have potential risk for public health in terms of VRE species. To our knowledge, this is the first study showing the presence of VRE in the clotted cream samples by PCR in Turkey.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the early diagnosability of superficial and visceral caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep isolated from C. pseudotuberculosis were compared with healthy sheep, it was determined that there was a statistically significant decrease in the levels of antioxidant molecules such as GSH-pX (14.62%), GSH (23.81%), SOD (4.70%), CAT (22.23%) (p˂0.001). The level of toxic malondialdehyde (MDA) (18.62%), the end product of lipid peroxidation, was found to be statistically significantly increased in sheep isolated from C. pseudotuberculosis (p˂0.001). As a result, it was determined that oxidative stress factors showed statistically significant variability in cases of superficial caseous lymphadenitis (caused by C.pseudotuberculosis). For this reason, by determining the levels of oxidative stress factors in suspected herds, it was possible to make early diagnosis of the superficial and visceral forms of caseous lymphadenitis, and a basis was established for future studies.
{"title":"Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors","authors":"E Polat, E Kaya, B Karagülle, H Akin","doi":"10.12681/jhvms.31218","DOIUrl":"https://doi.org/10.12681/jhvms.31218","url":null,"abstract":"In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the early diagnosability of superficial and visceral caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep isolated from C. pseudotuberculosis were compared with healthy sheep, it was determined that there was a statistically significant decrease in the levels of antioxidant molecules such as GSH-pX (14.62%), GSH (23.81%), SOD (4.70%), CAT (22.23%) (p˂0.001). The level of toxic malondialdehyde (MDA) (18.62%), the end product of lipid peroxidation, was found to be statistically significantly increased in sheep isolated from C. pseudotuberculosis (p˂0.001). As a result, it was determined that oxidative stress factors showed statistically significant variability in cases of superficial caseous lymphadenitis (caused by C.pseudotuberculosis). For this reason, by determining the levels of oxidative stress factors in suspected herds, it was possible to make early diagnosis of the superficial and visceral forms of caseous lymphadenitis, and a basis was established for future studies.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F Talazadeh, MH Razijalali, N Roshanzadeh, P Davoodi
Considering the increase in the keeping of pet birds and the importance of gastrointestinal parasitic infection, especially zoonotic parasites, this study was conducted to determine the prevalence of gastrointestinal parasites in different species of Passeriformes and Psittaciformes. In this study, one hundred and twenty fresh fecal samples were collected from different species of captive pet birds in Ahvaz from Apr 2021 to Aug 2021. The collected samples were analyzed using Clayton-Lane methods, Modified Ziehl–Neelsen, and Trichrome staining. Light microscopic morphometry was used for the identification of helminth eggs and oocysts. The results of this study showed that 37 samples (30.83%) were infected with gastrointestinal parasites. Among the helminthic parasites, three samples (2.5%) were infected with Hymenolepis spp.. Among protozoan parasites, 24 samples (20%) were infected with Eimeria spp., three samples (2.5%) were infected with Isospora spp., two samples (1.66%) were infected with Cryptosporidium spp., and five samples (4.16%) were infected with Giardia spp.. The results of the present study showed that the highest rate of gastrointestinal parasitic infection was in Passeriformes kept in pet shops and the prevalence of gastrointestinal parasitic infection was higher in the zebra finch, common mynah, and canary compared to other pet birds. But the highest rate of zoonotic parasitic infection was in Psittaciformes. According to the results, zoonotic parasites are present in pet birds in the Ahvaz area and should be considered by the owners of these pet birds, breeders, veterinarians, and public health organizations.
{"title":"Survey on the gastrointestinal parasites in Passeriformes and Psittaciformes with a focus on zoonotic parasites","authors":"F Talazadeh, MH Razijalali, N Roshanzadeh, P Davoodi","doi":"10.12681/jhvms.31398","DOIUrl":"https://doi.org/10.12681/jhvms.31398","url":null,"abstract":"Considering the increase in the keeping of pet birds and the importance of gastrointestinal parasitic infection, especially zoonotic parasites, this study was conducted to determine the prevalence of gastrointestinal parasites in different species of Passeriformes and Psittaciformes. In this study, one hundred and twenty fresh fecal samples were collected from different species of captive pet birds in Ahvaz from Apr 2021 to Aug 2021. The collected samples were analyzed using Clayton-Lane methods, Modified Ziehl–Neelsen, and Trichrome staining. Light microscopic morphometry was used for the identification of helminth eggs and oocysts. The results of this study showed that 37 samples (30.83%) were infected with gastrointestinal parasites. Among the helminthic parasites, three samples (2.5%) were infected with Hymenolepis spp.. Among protozoan parasites, 24 samples (20%) were infected with Eimeria spp., three samples (2.5%) were infected with Isospora spp., two samples (1.66%) were infected with Cryptosporidium spp., and five samples (4.16%) were infected with Giardia spp.. The results of the present study showed that the highest rate of gastrointestinal parasitic infection was in Passeriformes kept in pet shops and the prevalence of gastrointestinal parasitic infection was higher in the zebra finch, common mynah, and canary compared to other pet birds. But the highest rate of zoonotic parasitic infection was in Psittaciformes. According to the results, zoonotic parasites are present in pet birds in the Ahvaz area and should be considered by the owners of these pet birds, breeders, veterinarians, and public health organizations.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The nutritive value and invitro digestibility of agro-industrial byproducts of Citrus aurantium, Citrus paradisi, Citrus reticulata, Citrus limon and Citrus sinensis were assessed for their feed modification potential. Nutritive value assessment was done through chemical analysis while the ANKOM Daisy II incubator method was used to determine the invitro digestibility. At p>0.05, there was no statistical significance for nutritive value of the citrus byproducts for the five species. The agro-industrial by-products had significantly different phenolic content. Invitro digestibility of the peels significantly differed from the pomaces (p<0.001). The byproducts of the five citrus species showed potential to be considered as non-forage energy sources in ruminant feed. Lemon byproducts had high protein but not sufficient as a single protein source while bitter orange pomace may be considered for antioxidant capacity and high digestibility. Abundance of citrus agro-industrial byproducts, nutritive value potentials and their digestibility makes it a good strategy for ruminant feed modification and utilization.
{"title":"Nutritive value and in-vitro digestibility of peels and pomaces of different citrus species","authors":"OO Olowu, S Yaman Firincioglu","doi":"10.12681/jhvms.31044","DOIUrl":"https://doi.org/10.12681/jhvms.31044","url":null,"abstract":"The nutritive value and invitro digestibility of agro-industrial byproducts of Citrus aurantium, Citrus paradisi, Citrus reticulata, Citrus limon and Citrus sinensis were assessed for their feed modification potential. Nutritive value assessment was done through chemical analysis while the ANKOM Daisy II incubator method was used to determine the invitro digestibility. At p>0.05, there was no statistical significance for nutritive value of the citrus byproducts for the five species. The agro-industrial by-products had significantly different phenolic content. Invitro digestibility of the peels significantly differed from the pomaces (p<0.001). The byproducts of the five citrus species showed potential to be considered as non-forage energy sources in ruminant feed. Lemon byproducts had high protein but not sufficient as a single protein source while bitter orange pomace may be considered for antioxidant capacity and high digestibility. Abundance of citrus agro-industrial byproducts, nutritive value potentials and their digestibility makes it a good strategy for ruminant feed modification and utilization.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The goal of this study was to detect the influence of royal jelly which has a strong antioxidant capacity supplementation in TRIS egg yolk and TRIS soybean lecithin extenders on quality and fertility. Sperm was collected from 5 Damascus bucks via an electro ejaculator during the breeding season. After dilution and cooling, the lowest motility and membrane integrity were detected in TEY-1RJ, in terms of abnormal spermatozoon rate, the TEY-1RJ group was the highest in cooled semen (P<0.05). After dilution and cooling, osmolality decreased significantly when the RJ ratio increased (P<0.05). After freezing-thawing, in both TEY and TSL extenders, the lowest sperm quality was detected in the 1% RJ groups. In terms of intact acrosome ratio, the highest value was recorded in the 0.50% RJ group in TEY and TSL diluents (P<0.05). Fertility assessments were conducted with 150 goats synchronized with intravaginal sponges and goats were inseminated transcervical. There was no difference between pregnancy rates between TEY and TSL-control and RJ groups (P>0.05). As a result, it was deduced that RJ additions to TEY and TSL extenders did not produce any alternative to freezing on sperm quality in Damascus goat semen except for the intact acrosome ratio and had no positive effect on fertility.
{"title":"The influence of various rates of royal jelly enriched extenders on frozen/thawed sperm quality and fertility in goats","authors":"N Coskun Cetin, F Karaca","doi":"10.12681/jhvms.30798","DOIUrl":"https://doi.org/10.12681/jhvms.30798","url":null,"abstract":"The goal of this study was to detect the influence of royal jelly which has a strong antioxidant capacity supplementation in TRIS egg yolk and TRIS soybean lecithin extenders on quality and fertility. Sperm was collected from 5 Damascus bucks via an electro ejaculator during the breeding season. After dilution and cooling, the lowest motility and membrane integrity were detected in TEY-1RJ, in terms of abnormal spermatozoon rate, the TEY-1RJ group was the highest in cooled semen (P<0.05). After dilution and cooling, osmolality decreased significantly when the RJ ratio increased (P<0.05). After freezing-thawing, in both TEY and TSL extenders, the lowest sperm quality was detected in the 1% RJ groups. In terms of intact acrosome ratio, the highest value was recorded in the 0.50% RJ group in TEY and TSL diluents (P<0.05). Fertility assessments were conducted with 150 goats synchronized with intravaginal sponges and goats were inseminated transcervical. There was no difference between pregnancy rates between TEY and TSL-control and RJ groups (P>0.05). As a result, it was deduced that RJ additions to TEY and TSL extenders did not produce any alternative to freezing on sperm quality in Damascus goat semen except for the intact acrosome ratio and had no positive effect on fertility.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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