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In vitro acaricidal effect of Syzygium aromaticum and Cymbopogon citratus essential oil on engorged female of cattle tick Rhipicephalus microplus in Benin 香薷精油和香蒲精油对贝宁牛蜱雌虫的体外杀螨效果研究
Pub Date : 2016-03-25 DOI: 10.14196/SJVA.V5I3.2155
R. E. Yessinou, J. Adinci, P. Sessou, S. Adehan, Arétas Tonouweha, Y. Akpo, C. Adoligbe, M. Assogba, Benoît G. Koutinhouin, A. Youssao, S. Farougou
Ticks are external parasites of livestock and vector of several pathogens in infected animals. They lower production performances, which result in severe economic losses for breeders. So far a number of synthetic acaricide are used for the control of Rhipicephalus microplus. However, as a consequence of their extensive use, this tick specie has developed a resistance to the major classes of acaricide.  In order to limit resistance and reduce the invasion of this tick, we have evaluated the potential acaricide action of Syzygium aromaticum of Cymbopogon citratus essential oils and their mixture of engorged females Rhipicephalus microplus . The Adults Immersion Test carried out showed sensitivity of ticks to the extract of Syzygium aromaticum and Cymbopogon citratus essential oil: 100% mortality of engorged female was obtained at the highest concentration, 10mg/ml, for the two essential oils. The mixture of Syzygium aromaticum and Cymbopogon citratus oil induced 100% death at a concentration of 1.25mg / ml. The lethal doses 50, 90 and 99% of individuals was calculated. The highest oviposition inhibition rate (90.3% for Syzygium aromaticum and 60.7% for Cymbopogon citratus) was obtained at a concentration of 10mg/ml. However, no oviposition was seen from a concentration of 1.25mg /ml of the oil mixture. The results relevant to hatching reduction rate were similar. Hence, Syzygium aromaticum and Cymbopogon citratus essential oil mixture could be used as an alternative solution  in the fight against ticks.
蜱是家畜的外源寄生虫,也是受感染动物中几种病原体的媒介。它们降低了生产性能,给育种者造成了严重的经济损失。目前已有几种合成杀螨剂用于防治微头虫。然而,由于它们的广泛使用,这种蜱已经对主要种类的杀螨剂产生了抗药性。为了限制该蜱的抗药性,减少其入侵,本研究对柑桔香蒲精油及其混合制剂的潜在杀螨作用进行了评价。成虫浸泡试验表明,蜱对香薷精油和香椿精油提取物敏感,两种精油最高浓度为10mg/ml时,充血雌虫死亡率为100%。在浓度为1.25mg / ml的情况下,香茅与香茅油的混合剂量对大鼠的致死率为100%,分别为50%、90%和99%。当浓度为10mg/ml时,对香菇的产卵抑制率最高(90.3%),对香菇的产卵抑制率为60.7%。然而,在1.25mg /ml的混合油浓度下,没有观察到产卵。与孵化率相关的结果相似。因此,香薷与香橼精油混合物可作为对抗蜱虫的替代解决方案。
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引用次数: 17
Biodiversity, the green ecology, economics and ecosystem engineering 生物多样性,绿色生态学,经济学和生态系统工程
Pub Date : 2016-02-19 DOI: 10.14196/AA.V5I2.2126
A. Omer
The increased exploitation of renewable energy sources is central to any move towards sustainable development. However, casting renewable energy thus carries with it an inherent commitment to other basic tenets of sustainability, openness, democraticisations, etc. Due to increasing fossil fuel prices, the research in renewable energy technologies (RETs) utilisation has picked up a considerable momentum in the world. The present day energy arises has therefore resulted in the search for alternative energy resources in order to cope with the drastically changing energy picture of the world. The environmental sustainability of the current global energy systems is under serious question. A major transition away from fossil fuels to one based on energy efficiency and renewable energy is required. Alternatively energy sources can potentially help fulfill the acute energy demand and sustain economic growth in many regions of the world. The mitigation strategy of the country should be based primarily ongoing governmental programmes, which have originally been launched for other purposes, but may contribute to a relevant reduction of greenhouse gas emissions (energy-saving and afforestation programmes). Throughout the study several issues relating to renewable energies, environment and sustainable development are examined from both current and future perspectives. The exploitation of the energetic potential (solar and wind) for the production of electricity proves to be an adequate solution in isolated regions where the extension of the grid network would be a financial constraint.
增加对可再生能源的开发是任何朝向可持续发展的行动的核心。然而,铸造可再生能源同时也承载着对可持续性、开放性、民主化等其他基本原则的内在承诺。由于化石燃料价格的上涨,可再生能源技术(RETs)利用的研究在世界范围内取得了相当大的势头。因此,当今能源的兴起促使人们寻找替代能源,以应付世界能源格局的急剧变化。当前全球能源系统的环境可持续性受到严重质疑。我们需要从化石燃料转向基于能源效率和可再生能源的能源。另外,能源来源可能有助于满足世界许多地区的迫切能源需求并维持经济增长。该国的缓解战略应主要以正在进行的政府方案为基础,这些方案最初是为其他目的而启动的,但可能有助于减少温室气体排放(节能和植树造林方案)。在整个研究中,从当前和未来的角度审查了与可再生能源、环境和可持续发展有关的几个问题。利用能源潜力(太阳能和风能)发电证明是孤立地区的适当解决办法,在这些地区,扩大电网网络将是一项财政限制。
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引用次数: 0
Seroprevalence of Babesia bigemina and Anaplasma marginale in domestic animals of district Ganderbal 甘丹巴尔地区家畜双巴贝斯虫和边缘无形体的血清流行病学分析
Pub Date : 2016-01-19 DOI: 10.14196/SJVA.V5I1.2050
S. A. Rather, H. Tak, D. Kakru
The present study of seroprevalence of B. bigemina and A. marginale in cattle, sheep and goats was studied in Ganderbal district of Kashmir, between January to December 2012. A total of 153 blood samples were collected randomly from 40 cattle, 52 sheep and 61 goats for the preparation of blood smears and serum samples in four consecutive seasons and tested against B. bigemina and A. marginale using the SVANOVIR ® B. bigemina -Abs and A. marginale -Abs ELISA Kit. Samples were also examined by Giemsa's stained blood smear method. The effect of topography, season, age, gender and breed was observed in cattle during this study. The overall prevalence of B. bigemina infection was 3 (7.5%), 2 (3.84%) and 3 (4.91%) in cattle, sheep and goats and for A. marginale 2 (5%), 1 (1.92%) and1 (1.63%) respectively. The mixed infections between B. bigemina and A. marginale were 5% in cattle, 1.92% in sheep and 3.27% in goats. The seasonal prevalence of B.bigemina , A. marginale and mixed infection between them peaked in summer as revealed by blood smear examination and ELISA.
本研究于2012年1月至12月在克什米尔的Ganderbal地区对牛、绵羊和山羊的双双型双头螺旋体和边缘螺旋体的血清患病率进行了研究。采用SVANOVIR®B. bigemina -Abs和A. marginale -Abs酶联免疫吸附测定试剂盒,连续4个季节随机采集40头牛、52头绵羊和61头山羊153份血液标本,制备血涂片和血清。同时用吉姆萨染色血涂片法检测样品。考察了地形、季节、年龄、性别和品种对牛的影响。牛、绵羊和山羊的总感染率分别为3(7.5%)、2(3.84%)和3(4.91%),边缘螺旋体感染率分别为2(5%)、1(1.92%)和1(1.63%)。牛、绵羊和山羊的混合感染率分别为5%、1.92%和3.27%。血涂片检查和酶联免疫吸附试验显示,双头双歧杆菌和边缘双歧杆菌的季节性流行高峰在夏季。
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引用次数: 6
Expression of the bovine neutrophil β-defensins4 (BNBD4) and 10 (BNBD10), and β-defensin 1(DEFB1) in the bovine mammary gland with chronic mastitis by Staphylococcus aureus 牛中性粒细胞β-防御素4 (BNBD4)、10 (BNBD10)和β-防御素1(DEFB1)在慢性乳腺炎牛乳腺中的表达
Pub Date : 2016-01-19 DOI: 10.14196/SJVA.V5I1.2083
A. R. Tamayo-Sosa, Víctor Manuel del Villar-Perez, L. Manríquez, L. Tinoco-Gracia, J. A. Olivas-Valdez, T. Melgarejo-García
Bovine mastitis is the most costly infectious disease of dairy cattle worldwide and ischaracterized by inflammation of the mammary gland. The etiology of the pathogens influences the severity of the inflammation and the immune response, and specifically some elements of the innate immunity, like the antimicrobial peptides, are known to display some pathogen specificity. The antimicrobial peptides play an important role in the innate immune defense mechanisms of the mammary gland against mastitis causative microorganisms. In the present study the expression of three β -defensins was estimated across 5 localizations of the mammary gland infected with Sthapylococcus aureus , by quantitative real-time PCR. Copies of mRNA for BNBD10 and DEFB1 were detected in Lymph node, parenchyma, streak canal, cisterna, andRosette of Furstenberg, with the highest level observed in lymph node and the lowest in Rosette of Furstenberg, and the level of expression was higher for BNBD10 . The level of mRNA expression for BNBD4 was very low in all localizations, with respect to BNBD10 and DEFB1 . Therefore, BNBD10 and DEFB1 could play a key role in the mammary gland with mastitis by S. aureus .
牛乳腺炎是世界范围内最昂贵的奶牛传染病,其特征是乳腺炎症。病原体的病因影响炎症和免疫反应的严重程度,特别是先天免疫的一些成分,如抗菌肽,已知具有一定的病原体特异性。抗菌肽在乳腺对乳腺炎病原微生物的先天免疫防御机制中发挥重要作用。在本研究中,通过实时荧光定量PCR方法估计了金黄色葡萄球菌感染乳腺的5个部位中3种β防御素的表达。BNBD10和DEFB1 mRNA在弗斯汀宝的淋巴结、实质、条纹管、池池和andRosette中均有表达,其中淋巴结表达量最高,rossette表达量最低,BNBD10表达量较高。与BNBD10和DEFB1相比,BNBD4的mRNA表达水平在所有定位中都非常低。因此,BNBD10和DEFB1可能在金黄色葡萄球菌感染的乳腺乳腺炎中发挥关键作用。
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引用次数: 0
The Influence of Age, Sex and Breed on the Susceptibility to Trypanosomiasis in Cattle 年龄、性别和品种对牛锥虫病易感性的影响
Pub Date : 2016-01-01 DOI: 10.5455/jva.20160409124022
I. A, Midau A, O. E., Magaji M
A survey was conducted in Song Local Government Area of Adamawa State on Trypanosomiasis to determine the influence of age, sex and breed on the susceptibility to the infection. A total of 240 blood samples of different breeds, Sexes and ages of cattle were collected from randomly selected herd and analyzed in laboratory using wet, thick, thin smear and Buffy coat concentration techniques. An overall infection rate in males and females were 9.17% and 17.50% respectively, infection rates in Red Bororo, Adamawa Gudali and White Fulani were found to be 13.16%, 14.00% and 12.00% respectively. The result shows that Adamawa gudali has the highiest with White Fulani cattle having the lowest rate of infectioon. Trjpanosoma vivax infection was 9.17% and Tiypanosoma congolence was 4.16%. This result shows that the trypanosomiasis in the area is of great economic importance.Key words: Trypanosomiasis, age, sex, breed.
在阿达马瓦州宋地方政府辖区开展了一项关于锥虫病的调查,以确定年龄、性别和品种对感染易感性的影响。从随机选取的牛群中采集不同品种、性别和年龄的牛240份血液样本,采用湿法、厚法、薄涂片法和白毛浓缩法进行实验室分析。男性和女性的总感染率分别为9.17%和17.50%,红波罗罗、阿达马瓦古达利和白富拉尼的感染率分别为13.16%、14.00%和12.00%。结果表明,阿达马瓦古达利牛感染率最高,白富拉尼牛感染率最低。间日血吸虫感染率为9.17%,冠状体感染率为4.16%。这说明该地区的锥虫病具有重要的经济意义。关键词:锥虫病,年龄,性别,品种
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引用次数: 0
Waste Management Practices in Selected Poultry Farms in Umuahia, Abia State - 在阿比亚州Umuahia选定家禽养殖场的废物管理实践
Pub Date : 2016-01-01 DOI: 10.5455/JVA.20160916044806
E. Kalu, A. N. Ajaruonye, N. Okwara
This study examined the waste disposal system of poultry farmers in Abia state. Poultry waste generation is on the increase due to the increase in the number of poultry farms. Improper handling of these wastes can result in environmental pollution and also affect human health. A questionnaire survey was carried out in 26 selected poultry farms that practice deep litter management system. The results reveal a steady increase in the number of people involved in poultry farming in the state, most (61.8%) of which engaged in layer production. 73.1% of the farmers were not aware that improper disposal of their waste affects the environment and health of individuals. Other management practices investigated revealed that most of the farmers do not know how to handle their waste efficiently.
本研究调查了阿比亚州家禽养殖户的废物处理系统。由于家禽养殖场数量的增加,家禽废物的产生也在增加。这些废物处理不当会造成环境污染,也会影响人类健康。选取26个实行深度产仔管理制度的家禽养殖场进行问卷调查。结果显示,该州从事家禽养殖的人数稳步增加,其中大多数(61.8%)从事蛋鸡生产。73.1%的农民不知道废物处置不当会影响环境和个人健康。调查的其他管理实践显示,大多数农民不知道如何有效地处理他们的废物。
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引用次数: 4
Perception of Rabies among Residents of Selected Local Government Areas of Oyo State, Nigeria 尼日利亚奥约州选定地方政府区域居民对狂犬病的看法
Pub Date : 2016-01-01 DOI: 10.5455/JVA.196912310400000
O. Adejumobi, O. Omobowale, S. Olaogun, T. Omobowale, H. O. Nottidge
Rabies, a fatal neurotropic disease of man and animals is usually a neglected disease in many parts of the world probably because of the erroneous belief that it affects animals predominantly. This study was designed to investigate the knowledge and attitude of people in selected communities of Oyo State, Nigeria with the aim of assessing the possible risk of getting infected with the virus. A total of 323 participants were randomly selected from 10 local government areas of Oyo State, Nigeria and interviewed using a pre-tested structured questionnaire. The results were analysed using descriptive statistics and Pearsons chi square method. Information obtained included the demographic characteristics of the participants, their interaction with dogs, knowledge about rabies and its fatality, its mode of transmission and prevention as well as the perception of and attitude of people towards rabies. Of the 323 participants, 180 (55.8%) were male while 143 (44.3%) were female, 115 (47.7%) were Christians, 123 (51%) were muslims and 3 (1.2%) were traditional religion adherents. Furthermore, 263 (81.4%) had different levels of education 60 (18.6 %) were illiterate, 119 (36.8%) used to own dog while only 54 (16.4%) presently kept dogs for reasons which included hunting 16 (30.1%), security 28 (52.8%), breeding {3 (5.66%)} and as companionship 6 (11.3%). Greater number (80%) lived in the rural areas while 62 (19.6%) were urban dwellers. Out of the 323 participants, 256 (79.5%) knew about rabies, of these, 46 (18%) heard about the disease from awareness campaigns, 11 (4.3%) heard from human and veterinary hospitals 58 (22.7%) heard from interpersonal interactions while 123 (48.1%) knew by personal experiences. From the people that claimed knowledge, 231 (90.2%) knew that rabies was a fatal disease, 96 (37.5%) knew about rabies in other animal species aside dogs, 110 (43%) knew about rabies transmission by dog or cat bite, 24 (9.4%) knew that rabies can be transmitted by wild animals. Also, 130 (50.8%) claimed knowledge of rabies prevention in animals but 88 (34.4%) only knew about antirabies vaccination in animals. Similarly, 128 (50%) claimed knowledge of rabies prevention in man and of these, 38 (14.8%) recommended prophylactic and post exposure antirabies vaccination, 42 (16%) hospital visits, 20 (7.8%) dog elimination and staying away from dogs, while 12 (4.7%) recommended religious means, and use of herbs. When asked about first aid treatment, 99 (38.7%) suggested wound washing with soap and antiseptic, 171 (66.8%) recommended hospital visit while 40 (15.6%) suggested a visit to the herbalist for the antidote. Pearsons chi square showed significant difference (PKey words: Rabies, perception, attitude.
狂犬病是人类和动物的一种致命的嗜神经疾病,在世界许多地方通常被忽视,这可能是因为人们错误地认为它主要影响动物。这项研究的目的是调查尼日利亚奥约州选定社区中人们的知识和态度,以评估感染该病毒的可能风险。从尼日利亚奥约州的10个地方政府区随机抽取了323名参与者,并使用预先测试的结构化问卷进行了访谈。采用描述性统计和皮尔逊卡方方法对结果进行分析。获得的信息包括参与者的人口统计特征、他们与狗的互动、对狂犬病及其致死率的认识、狂犬病的传播方式和预防,以及人们对狂犬病的看法和态度。在323名参与者中,男性180人(55.8%),女性143人(44.3%),基督徒115人(47.7%),穆斯林123人(51%),传统宗教信徒3人(1.2%)。此外,263人(81.4%)受过不同程度的教育,60人(18.6%)不识字,119人(36.8%)曾经养狗,而目前养狗的原因只有54人(16.4%),其中包括狩猎16人(30.1%),安全28人(52.8%),饲养3人(5.66%)和陪伴6人(11.3%)。更多的人(80%)生活在农村地区,而62人(19.6%)是城市居民。在323名参与者中,256人(79.5%)了解狂犬病,其中46人(18%)从宣传活动中听说过狂犬病,11人(4.3%)从人类和兽医医院听说过狂犬病,58人(22.7%)从人际交往中听说过狂犬病,123人(48.1%)通过个人经历知道狂犬病。在认知者中,231人(90.2%)知道狂犬病是一种致命疾病,96人(37.5%)知道除犬外的其他动物的狂犬病,110人(43%)知道狂犬病通过狗或猫咬伤传播,24人(9.4%)知道狂犬病可以通过野生动物传播。130人(50.8%)表示了解动物狂犬病预防知识,88人(34.4%)仅了解动物抗狂犬病疫苗接种。同样,128人(50%)声称了解人类狂犬病预防,其中38人(14.8%)建议预防性和接触后抗狂犬病疫苗接种,42人(16%)去医院就诊,20人(7.8%)消灭狗和远离狗,而12人(4.7%)建议宗教手段和使用草药。当被问及急救处理时,99名(38.7%)建议用肥皂和消毒剂清洗伤口,171名(66.8%)建议去医院,40名(15.6%)建议去看草药医生解毒剂。关键词:狂犬病,感知,态度。
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引用次数: 2
Serological Survey for Brucella Antibodies in Horses in Borno State, Nigeria - 尼日利亚博尔诺州马布鲁氏菌抗体血清学调查
Pub Date : 2016-01-01 DOI: 10.5455/JVA.20160324081434
A. Tijjani, A. Junaidu, M. D. Salihu, A. Farouq, O. Faleke, S. Adamu
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引用次数: 1
Survey of Haemoparasites and Haematology of Scavenging Ducks (Anas Platyhyncha) in Owerri Southeastern Nigeria 尼日利亚东南部奥韦里食腐鸭(Anas Platyhyncha)血液寄生虫和血液学调查
Pub Date : 2016-01-01 DOI: 10.5455/jva.1969123104000001
M. Opara, F. C. Oguobi, E. E. Adiele, O. Jegede
A study was carried out to investigate the haematological parameters and haemoparasites of naturally infected adult scavenging ducks in Owerri, southeastern Nigeria. Blood samples were collected from them for haematology and parasitological examinations. Out of the 200 ducks examined, 120 (40%) were infected with haemoparasites. Of these, 100 (83.3%) were infected with Leucocytozoon, while 20 (16.7%) had Trypanosoma infection. PCV and Hb values of the non – infected ducks were significantly (p < 0.05) higher than the infected ones. Their values of the RBC, MCV and MCH were equally higher, but not significant (p > 0.05). WBC, Neutrophils, Eosinophils and Lymphocytes were higher (p < 0.05) in the infected than non – infected ducks. Across sexes, infection in the 40 (33.3%) drakes was accounted for solely by Leucocytozoon, while the 80 (66.7%) infected ducks had 75% and 25% infections by Leucocytozoon and Trypanosoma organisms respectively. The male duck (drake) had significantly higher total WBC count (p<0.05) but significantly lower RBC count (p<0.05). However, the values of PCV, Hb, MCH, MCHC and MCV were similar in the two sexes. It was concluded that Leucocytozoon and Trypanosoma species are prevalent among scavenging local ducks reared in Owerri, Imo state and could be responsible for the microcytic normochromic anaemia and leucocytosis among these birds, thus, adversely affecting their productivity.
对尼日利亚东南部奥韦里自然感染的成年食腐鸭的血液学参数和血液寄生虫进行了研究。从他们身上采集血样进行血液学和寄生虫学检查。在检查的200只鸭子中,120只(40%)感染了血寄生虫。其中白细胞原虫感染100例(83.3%),锥虫感染20例(16.7%)。未感染鸭的PCV和Hb值显著高于感染鸭(p < 0.05)。RBC、MCV、MCH值均高于对照组,但差异无统计学意义(p < 0.05)。感染鸭的白细胞、中性粒细胞、嗜酸性粒细胞和淋巴细胞高于未感染鸭(p < 0.05)。从性别上看,40只鸭(33.3%)只感染了白细胞虫,80只鸭(66.7%)感染了白细胞虫和锥虫,分别占75%和25%。公鸭总白细胞计数显著高于对照组(p<0.05),红细胞计数显著低于对照组(p<0.05)。而PCV、Hb、MCH、MCHC和MCV的值在两性中是相似的。综上所述,在伊莫州Owerri饲养的食腐鸭中,白细胞虫和锥虫种类普遍存在,可能是造成这些鸭的小细胞正色贫血和白细胞增多症的原因,从而对它们的生产产生不利影响。
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引用次数: 4
Serological Investigation of Heartworm (Dirofilaria immitis) Infection in Military Dogs from Rio de Janeiro, Brazil - 巴西里约热内卢军犬心丝虫感染血清学调查
Pub Date : 2016-01-01 DOI: 10.5455/jva.19691231040000001
G. L. G. Almeida, M. B. Almeida, A. C. M. Santos, Â. V. Mattos, A. C. Oliveira, R. Barros, V. D. S. Campos, W. N. Souza, A. Balthazar, M. Lautenschlager
Paulo. Abstract This study was designed to find out the seroprevalence of heartworm infection in working military dogs from the metropolitan area of Rio de Janeiro, Brazil. During May, 2015 to June 2016, blood samples from 103 asymptomatic active working dogs from Air Force, Army and Marine Corps were submitted to immunochromatographic rapid test assay for Dirofilaria immitis infection . The study included the whole dog population housed in four military kennels located in different neighborhoods of Rio de Janeiro and São Gonçalo cities. The research results revealed an overall mean prevalence of 5.82%. There was no gender influence on the frequency of the infection (p>0.05). The mean age of positive dogs (5.3 years) was higher than the negative ones (3.3 years) (p<0.05). Occult infection was documented in 16.6% of the seropositive
保罗。摘要本研究旨在了解巴西里约热内卢市区工作军犬心丝虫的血清感染情况。2015年5月至2016年6月,对美国空军、陆军和海军陆战队现役无症状工作犬103只进行免疫层析快速检测。这项研究包括了在里约热内卢和奥戈萨罗塔罗市不同社区的四个军事狗舍里饲养的所有狗。研究结果显示,总体平均患病率为5.82%。性别对感染频率无显著影响(p>0.05)。阳性犬平均年龄(5.3岁)高于阴性犬平均年龄(3.3岁)(p<0.05)。16.6%的血清阳性患者存在隐匿性感染
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引用次数: 0
期刊
Journal of Veterinary Advances
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