Journal of Traditional and Complementary Medicine最新文献_第9页

Traditional Chinese medicine as a protective strategy against chemotherapy-induced cardiotoxicity: An overview of the literature 中药作为化疗引起的心脏毒性的保护策略：文献综述

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-06-22 DOI: 10.1016/j.jtcme.2024.06.010

Jun-Wei Wang , Hong Shao , You-Ni Zhang , Tong Ge , Xiao-Yi Chen , Xiao-Zhou Mou

Cardiotoxicity refers to the damage caused to the heart or vascular system, which has become a significant cause of morbidity and mortality worldwide. Up to 20 % of adults undergoing cancer treatment may experience cardiotoxicity, with 7 %–10 % developing cardiomyopathy or heart failure. Cardiotoxic medications put patients at risk for a variety of heart conditions, including cardiac failure (HF), left ventricle (LV) systolic malfunction, cardiac arrhythmia, hypertension, coronary artery disease (CAD), and ischemia/myocardial infarction. These diseases may significantly impact the patient's quality of life and outcomes. Many conventional cancer chemotherapy agents have serious adverse effects. Anthracyclines, alkylating agents, taxanes, topoisomerase inhibitors, and antimetabolites are chemotherapeutic agents that induce cardiotoxicity. Recently, there has been an increasing focus on using traditional Chinese medicine (TCM) to treat chemotherapy-induced cardiotoxicity. TCM encompasses a range of practices, such as herbology, acupuncture, dietary therapy, and physical exercises like t'ai chi. These practices aim to enhance the flow of qi, which can alleviate symptoms of illness. Although the effectiveness of TCM has not been extensively researched and supported, scientists have begun utilizing scientific methods to gain a deeper understanding of the mechanisms underlying TCM. Ongoing research is being conducted to determine the safety and effectiveness of TCM treatments in reducing chemotherapy-induced toxicity. The present study aims to evaluate the protection and efficacy of various TCM treatments in chemotherapy-induced cardiotoxicity.

心脏毒性是指对心脏或血管系统造成的损害，它已成为世界范围内发病率和死亡率的重要原因。在接受癌症治疗的成年人中，高达20%的人可能会出现心脏毒性，7% - 10%的人会出现心肌病或心力衰竭。心脏毒性药物使患者面临各种心脏疾病的风险，包括心力衰竭（HF）、左心室（LV）收缩功能障碍、心律失常、高血压、冠状动脉疾病（CAD）和缺血/心肌梗死。这些疾病可能会严重影响患者的生活质量和预后。许多传统的癌症化疗药物都有严重的副作用。蒽环类药物、烷基化剂、紫杉烷、拓扑异构酶抑制剂和抗代谢物是诱导心脏毒性的化疗药物。近年来，人们越来越关注使用中药治疗化疗引起的心脏毒性。中医包括一系列的实践，如草药学、针灸、饮食疗法和像太极拳这样的体育锻炼。这些做法旨在加强气的流动，这可以减轻疾病的症状。虽然中药的有效性还没有得到广泛的研究和支持，但科学家们已经开始利用科学的方法来更深入地了解中药的潜在机制。目前正在进行研究，以确定中药治疗在减少化疗引起的毒性方面的安全性和有效性。本研究旨在评价各种中药治疗方法对化疗性心脏毒性的保护作用和疗效。

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引用次数: 0

Guilingji capsules enhances erectile function by promoting testosterone-dependent angiogenesis in the corpus cavernosum 龟苓姬胶囊通过促进海绵体中睾酮依赖性血管生成而增强勃起功能

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-06-18 DOI: 10.1016/j.jtcme.2024.06.009

Xujun Yu , Jingyi Zhang , Suyun Xu , Jing Zhou , Chenglin Zhuang , Junjun Li , Baojun Zhuang

Background and aim

Guilingji capsules (GLJC) have the effect of treating erectile dysfunction (ED). This study aims to explore the potential mechanisms of GLJC in treating ED.

Experimental procedure

We conducted network pharmacology analysis of ED-related targets with GLJC components reported in the TCMSP database and GLJC components reported in the literature, respectively. Molecular docking was employed to validate the binding affinity of these molecular targets. Animal experiments were conducted to validate the aforementioned results. The mechanism of GLJC in treating ED was studied using d-galactose-induced aging rats, and orchiectomized rats were used to investigate further whether the mechanism of GLJC in treating ED is related to androgen.

Results and conclusions

Two network pharmacology analyses indicated that Androgen receptor (AR) and fibroblast growth factor 2 (FGF2) are the candidate targets, suggesting that the mechanism of ED treatment by GLJC may be related to androgens and angiogenesis. Molecular docking further validated the effective binding of GLJC components to these two targets. In animal experiments, GLJC significantly increased the frequency of erections and elevated serum free testosterone levels and penile tissue AR expression in aged rats. GLJC also promoted angiogenesis and inhibited penile tissue fibrosis in aged rats by regulating the expression of FGF2, RICTOR/P-AKT/P-FOXO1. However, such regulation was not observed in orchiectomized rats. Therefore, GLJC increased testosterone utilization in d-galactose-induced aging rats and regulated FGF2, RICTOR/P-AKT/P-FOXO1 signaling pathway in a T-dependent manner, promoting corpus cavernosum survival and angiogenesis. This mechanism led to the inhibition of penile fibrosis.

背景及目的：贵灵鸡胶囊具有治疗勃起功能障碍（ED）的作用。本研究旨在探讨GLJC治疗ed的潜在机制。实验步骤：我们分别用TCMSP数据库中报道的GLJC成分和文献中报道的GLJC成分对ed相关靶点进行网络药理学分析。通过分子对接验证这些分子靶点的结合亲和力。动物实验验证了上述结果。以d-半乳糖诱导的衰老大鼠为实验对象研究GLJC治疗ED的机制，并以去睾丸大鼠为实验对象进一步探讨GLJC治疗ED的机制是否与雄激素有关。结果和结论两项网络药理学分析显示雄激素受体（AR）和成纤维细胞生长因子2 （FGF2）是候选靶点，提示GLJC治疗ED的机制可能与雄激素和血管生成有关。分子对接进一步验证了GLJC组分与这两个靶点的有效结合。在动物实验中，GLJC显著增加了老龄大鼠的勃起频率，提高了血清游离睾酮水平和阴茎组织AR表达。GLJC还通过调节FGF2、RICTOR/P-AKT/ p - fox01的表达，促进老年大鼠血管生成，抑制阴茎组织纤维化。然而，在切除睾丸的大鼠中没有观察到这种调节。因此，GLJC增加了d-半乳糖诱导衰老大鼠的睾酮利用，并以t依赖的方式调节FGF2、RICTOR/P-AKT/P-FOXO1信号通路，促进海肌体存活和血管生成。这一机制导致了阴茎纤维化的抑制。

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引用次数: 0

Potent anti-biofilm properties of plumbagin against fluconazole-resistant Candida auris plumbagin 对抗氟康唑念珠菌的强效抗生物膜特性

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-06-10 DOI: 10.1016/j.jtcme.2024.06.005

Hye-Won Jin , Yong-Bin Eom

Background and aim

The escalation of fungal infections is driving an increase in disease and mortality rates. In particular, the emergence of Candida auris (C. auris), which shows powerful resistance to the antifungal drug fluconazole, is becoming a global concern. Furthermore, several biological hurdles need to be overcome by candidate therapeutics because C. auris has the ability to form biofilm. Therefore, this study aimed to investigate the antifungal and anti-biofilm effects of plumbagin, a natural extract, against fluconazole-resistant C. auris (FRCA).

Experimental procedure

The minimum inhibitory concentrations (MICs) of fluconazole and plumbagin were determined against clinically isolated C. auris. Inhibition of biofilm formation and eradication effects of plumbagin against FRCA were confirmed through minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) assays. Additionally, the inhibition of metabolic activity in biofilm cells was verified through quantification by XTT reduction assay and visualization by confocal laser scanning microscopy (CLSM). The relative expression levels of the azole resistant gene ERG11, the efflux pump gene CDR1, and the extracellular matrix gene KRE6, were measured.

Results and conclusion

Plumbagin exhibits antifungal efficacy against C. auris and has been shown to effectively inhibit both the formation and eradication of biofilms produced by FRCA. Furthermore, the metabolic activity inhibition in biofilm cells was both quantified and visually observed. The downregulation of all genes (ERG11, CDR1, and KRE6) by plumbagin was confirmed. Taken together, this study demonstrates that plumbagin has antifungal and anti-biofilm efficacy against FRCA, indicating its potential as an alternative to antifungal agents and a valuable resource in combating FRCA infections.

背景与目的真菌感染的升级正在推动疾病和死亡率的增加。特别是，对抗真菌药物氟康唑表现出强大耐药性的耳念珠菌（C. auris）的出现正成为全球关注的问题。此外，候选治疗方法需要克服几个生物学障碍，因为金黄色葡萄球菌具有形成生物膜的能力。因此，本研究旨在研究天然提取物白桦素对耐氟康唑金黄色葡萄球菌（FRCA）的抗真菌和抗生物膜作用。实验方法测定氟康唑和白丹素对临床分离的金黄色葡萄球菌的最低抑菌浓度（mic）。通过最小生物膜抑制浓度（MBIC）和最小生物膜根除浓度（MBEC）测定，证实了白桦素对FRCA的生物膜形成和根除作用。此外，通过XTT还原法定量和共聚焦激光扫描显微镜（CLSM）可视化验证了生物膜细胞代谢活性的抑制作用。测定抗唑基因ERG11、外排泵基因CDR1和细胞外基质基因KRE6的相对表达量。结果与结论白丹素对金黄色葡萄球菌具有一定的抗真菌作用，并能有效抑制FRCA生物膜的形成和清除。此外，对生物膜细胞的代谢活性抑制进行了定量和目视观察。证实白桦素下调了所有基因（ERG11、CDR1和KRE6）。综上所述，本研究表明白桦白素对FRCA具有抗真菌和抗生物膜的作用，表明其作为抗真菌药物的替代品和对抗FRCA感染的宝贵资源的潜力。

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引用次数: 0

Crocin elicits potent anti-inflammatory and fibrinolytic properties post tendon injury, A new molecule for adhesion therapy 肌腱损伤后，克罗霉素具有强效抗炎和纤维蛋白溶解特性--一种用于粘连治疗的新分子

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-06-09 DOI: 10.1016/j.jtcme.2024.06.001

Background

Post-surgical tendon adhesion formation is a frequent clinical complication with limited treatment options. The aim of this study is to investigate safety and efficacy of orally administration of crocin in attenuating post-operative tendon-sheath adhesion bands in an Achilles tendon rat model.

Methods

Structural, mechanical, histological, and biochemical properties of Achilles tendons were analyzed in the presence and absence of crocin. Inflammation and total fibrosis of tendon tissues were graded between groups using macroscopic and histological scoring methods.

Results

Crocin significantly alleviated the severity, length, and density of Achilles tendon adhesions. Moreover, the recruitment of inflammatory cells and inflammation were significantly decreased in post-operative tissue samples of the crocin-treated group, as quantified with Moran scoring system. Histological results showed that crocin elicited a potent anti-fibrotic effect on tendon tissue samples as visualized by decreasing quantity, quality, grading of fibers, and collagen deposition at the site of surgery when scored either by Tang or Ishiyama grading systems. The H&E staining showed no histo-pathological changes or damage to heart, kidney, and liver tissues of treated rats.

Conclusion

Our results showed that crocin is a safe effective therapeutic candidate with potent anti-inflammatory and anti-fibrotic properties for adhesion band therapy post tendon surgery.

背景手术后肌腱粘连是一种常见的临床并发症，但治疗方法有限。本研究旨在探讨在跟腱大鼠模型中口服巴豆苷以减轻术后腱鞘粘连带的安全性和有效性。方法分析巴豆苷存在和不存在时跟腱的结构、机械、组织学和生化特性。采用宏观和组织学评分方法对不同组间肌腱组织的炎症和总纤维化程度进行分级。此外，根据 Moran 评分系统的量化结果，术后跟腱粘连组组织样本中炎症细胞的募集和炎症明显减少。组织学结果表明，使用 Tang 或 Ishiyama 分级系统进行评分时，手术部位纤维的数量、质量、分级和胶原沉积均有所减少，由此可见，巴豆苷对肌腱组织样本具有强效的抗纤维化作用。H&E染色显示，治疗大鼠的心脏、肾脏和肝脏组织未发生组织病理学变化或损伤。结论我们的研究结果表明，巴豆苷是一种安全有效的候选疗法，具有强大的抗炎和抗纤维化特性，可用于肌腱手术后的粘连带治疗。

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引用次数: 0

Auricular acupuncture plays a neuroprotective role in 6-hydroxydopamine-induced Parkinson's disease in rats 耳穴针刺对6-羟多巴胺诱导的帕金森病大鼠具有神经保护作用

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-28 DOI: 10.1016/j.jtcme.2024.05.008

Huong Thi-Mai Nguyen , Der-Yen Lee , Ching-Liang Hsieh

Background

Parkinson's disease (PD) is the second-most common neurodegenerative disease. Currently, PD treatment is symptomatic and involves the use of dopamine-based therapies. This study investigated auricular acupuncture on motor and cognitive abilities in rats with 6-OHDA-induced PD.

Methods

A PD rat model was established by bilaterally injecting 6-OHDA into the lateral dorsal striatum. Then, 2- or 15-Hz auricular electroacupuncture (EA) was applied to the auricular CO15 and CO12 points bilaterally for 20 min three times a week for four consecutive weeks.

Results

Both the latency to fall and rest time of the open field test in the EA15 group were greater than in the 6-OHDA group (p < 0.001 and p < 0.05). The time spent on the two-object recognition task was greater in the EA15 group and EA2 group than in the 6-OHDA group (p < 0.01 and p < 0.05). More tyrosine hydroxylase (TH)-positive neurons and fibers were noted in the dorsolateral striatum and substantia nigra (SN) (all p < 0.05). TH expression in the SN was greater in the EA15 group than that in the 6-OHDA group (p < 0.05), while α-synuclein expression in the SN was stronger in the 6-OHDA group than in the EA15 group (p < 0.05). The l-DOPA level in the striatum was higher in the EA15 group than in the 6-OHDA group (p < 0.05).

Conclusion

According to the results, rats with 6-OHDA-induced PD may benefit from auricular EA in terms of motor and cognitive behavior as well as neuroprotection.

帕金森病（PD）是第二常见的神经退行性疾病。目前，帕金森病的治疗是对症的，涉及到以多巴胺为基础的治疗。本研究探讨耳针对6-羟多巴胺诱导的帕金森病大鼠运动和认知能力的影响。方法采用双侧侧背纹状体注射6-羟色胺建立PD大鼠模型。然后，每周3次或15 hz耳穴电针（EA）在耳穴CO15和CO12穴双侧穿刺20 min，连续4周。结果EA15组裸地试验跌倒潜伏期和休息时间均大于6-OHDA组(p <；0.001和p <；0.05)。EA15组和EA2组在双目标识别任务上花费的时间大于6-OHDA组(p <；0.01和p <；0.05)。背外侧纹状体和黑质（SN）中酪氨酸羟化酶（TH）阳性的神经元和纤维较多(p <；0.05)。EA15组SN中TH的表达高于6-OHDA组(p <；0.05)， 6-OHDA组SN中α-synuclein的表达强于EA15组(p <；0.05)。EA15组纹状体左旋多巴水平高于6-OHDA组(p <；0.05)。结论6-羟多巴胺诱导的帕金森病大鼠在运动、认知行为和神经保护方面可能受益于耳穴电刺激。

{"title":"Auricular acupuncture plays a neuroprotective role in 6-hydroxydopamine-induced Parkinson's disease in rats","authors":"Huong Thi-Mai Nguyen , Der-Yen Lee , Ching-Liang Hsieh","doi":"10.1016/j.jtcme.2024.05.008","DOIUrl":"10.1016/j.jtcme.2024.05.008","url":null,"abstract":"<div><h3>Background</h3><div>Parkinson's disease (PD) is the second-most common neurodegenerative disease. Currently, PD treatment is symptomatic and involves the use of dopamine-based therapies. This study investigated auricular acupuncture on motor and cognitive abilities in rats with 6-OHDA-induced PD.</div></div><div><h3>Methods</h3><div>A PD rat model was established by bilaterally injecting 6-OHDA into the lateral dorsal striatum. Then, 2- or 15-Hz auricular electroacupuncture (EA) was applied to the auricular CO15 and CO12 points bilaterally for 20 min three times a week for four consecutive weeks.</div></div><div><h3>Results</h3><div>Both the latency to fall and rest time of the open field test in the EA15 group were greater than in the 6-OHDA group (<em>p</em> < 0.001 and <em>p</em> < 0.05). The time spent on the two-object recognition task was greater in the EA15 group and EA2 group than in the 6-OHDA group (<em>p</em> < 0.01 and <em>p</em> < 0.05). More tyrosine hydroxylase (TH)-positive neurons and fibers were noted in the dorsolateral striatum and substantia nigra (SN) (all <em>p</em> < 0.05). TH expression in the SN was greater in the EA15 group than that in the 6-OHDA group (<em>p</em> < 0.05), while α-synuclein expression in the SN was stronger in the 6-OHDA group than in the EA15 group (<em>p</em> < 0.05). The <span>l</span>-DOPA level in the striatum was higher in the EA15 group than in the 6-OHDA group (<em>p</em> < 0.05).</div></div><div><h3>Conclusion</h3><div>According to the results, rats with 6-OHDA-induced PD may benefit from auricular EA in terms of motor and cognitive behavior as well as neuroprotection.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 2","pages":"Pages 128-139"},"PeriodicalIF":3.3,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jieduquyuziyin prescription attenuates the side effect of prednisone through regulating gut microbiota when in the combination with prednisone treat MRL/lpr mice 洁尔阴处方与泼尼松联合治疗MRL/lpr小鼠时，通过调节肠道微生物群减轻泼尼松的副作用

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-24 DOI: 10.1016/j.jtcme.2024.05.005

Zhengyang Zhu , Mingzhu Wang , Lin Huang , Zhixing He

Jieduquyuziyin prescription (JP) is an empirical formula used to treat systemic lupus erythematosus (SLE). While JP has been shown to have synergistic and attenuated effects when combined with glucocorticoids (GCs) for SLE treatment, the precise mechanism remains unclear. This study utilized MRL/lpr mice to demonstrate the synergistic and attenuated effects of JP when combined with prednisone. Furthermore, a co-housing experiment was conducted to investigate whether JP-regulated gut microbiota had synergistic and attenuated effects in prednisone-treated MRL/lpr mice. The study found that JP exhibited synergistic effects only when combined with 5 mg/kg body weight prednisone, while its attenuated effects were observed with both 5 and 10 mg/kg body weight prednisone. Co-housing resulted in the transmission of gut microbiota between prednisone-treated and JP-treated MRL/lpr mice. However, co-housing did not enhance the therapeutic efficacy of prednisone; instead, it attenuated prednisone's adverse effects on liver inflammation (IL-6 and TNF-α) and serum cholesterol in MRL/lpr mice. The attenuated effects of JP may be associated with specific genera such as Akkermansia, Parasutterella, and Alistipes. These findings suggest that JP can mitigate the adverse effects of GCs by modulating gut microbiota during the treatment of SLE with GCs.

解毒祛瘀滋饮方是治疗系统性红斑狼疮（SLE）的经验方。虽然JP与糖皮质激素（GCs）联合治疗SLE具有协同和减弱作用，但确切的机制尚不清楚。本研究利用MRL/lpr小鼠来证明JP与强的松联合使用时的协同和减弱作用。此外，研究人员还进行了一项共舍实验，以研究jp调节的肠道微生物群在强的松治疗的MRL/lpr小鼠中是否具有协同和减毒作用。本研究发现，JP仅在与5mg /kg体重的强的松联合使用时才表现出协同作用，而在与5mg /kg体重的强的松联合使用时，JP均表现出减弱作用。共居导致泼尼松治疗和jp治疗的MRL/lpr小鼠之间肠道微生物群的传递。然而，共住房并没有提高强的松的治疗效果；相反，它减轻了强的松对MRL/lpr小鼠肝脏炎症（IL-6和TNF-α）和血清胆固醇的不良影响。JP的减弱效应可能与特定属有关，如Akkermansia， Parasutterella和Alistipes。这些研究结果表明，JP可以通过调节GCs治疗SLE期间的肠道微生物群来减轻GCs的不良反应。

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引用次数: 0

In vivo evaluation of Andrographis paniculata and Boesenbergia rotunda extract activity against SARS-CoV-2 Delta variant in Golden Syrian hamsters: Potential herbal alternative for COVID-19 treatment 穿心莲和苧麻提取物对金色叙利亚仓鼠 SARS-CoV-2 Delta 变异株的体内活性评估：COVID-19治疗的潜在草药替代品

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-16 DOI: 10.1016/j.jtcme.2024.05.004

The ongoing COVID-19 pandemic has triggered extensive research, mainly focused on identifying effective therapeutic agents, specifically those targeting highly pathogenic SARS-CoV-2 variants. This study aimed to investigate the in vivo antiviral efficacy and anti-inflammatory activity of herbal extracts derived from Andrographis paniculata and Boesenbergia rotunda, using a Golden Syrian hamster model infected with Delta, a representative variant associated with severe COVID-19. Hamsters were intranasally inoculated with the SARS-CoV-2 Delta variant and orally administered either vehicle control, B. rotunda, or A. paniculata extract at a dosage of 1000 mg/kg/day. Euthanasia was conducted on days 1, 3, and 7 post-inoculation, with 4 animals per group. The results demonstrated that oral administration of A. paniculata extract significantly alleviated both lethality and infection severity compared with the vehicle control and B. rotunda extract. However, neither extract exhibited direct antiviral activity in terms of reducing viral load in the lungs. Nonetheless, A. paniculata extract treatment significantly reduced IL-6 protein levels in the lung tissue (7278 ± 868.4 pg/g tissue) compared to the control (12,495 ± 1118 pg/g tissue), indicating there was a decrease in local inflammation. This finding is evidenced by the ability of A. paniculata extract to reduce histological lesions in the lungs of infected hamsters. Furthermore, both extracts significantly decreased IL-6 and IP-10 mRNA expression in peripheral blood mononuclear cells of infected hamsters compared to the control group, suggesting systemic anti-inflammatory effects occurred. In conclusion, A. paniculata extract's potential therapeutic application for SARS-CoV-2 arises from its observed capacity to lessen inflammatory cytokine concentrations and mitigate lung pathology.

正在进行的 COVID-19 大流行引发了广泛的研究，主要集中在确定有效的治疗药物，特别是那些针对高致病性 SARS-CoV-2 变体的药物。本研究旨在利用金色叙利亚仓鼠感染 Delta（一种与严重 COVID-19 相关的代表性变异体）模型，研究穿心莲和苧麻提取物的体内抗病毒功效和抗炎活性。给仓鼠鼻内接种 SARS-CoV-2 Delta 变异株，然后口服车辆对照、穿心莲或穿心莲提取物，剂量为 1000 毫克/千克/天。在接种后的第 1、3 和 7 天对每组 4 只动物实施安乐死。结果表明，与载体对照和罗汉果提取物相比，口服罗汉果提取物可显著降低致死率和感染严重程度。然而，这两种提取物都没有表现出直接的抗病毒活性，即减少肺部的病毒载量。然而，与对照组（12495 ± 1118 pg/g组织）相比，A. paniculata提取物处理可显著降低肺组织中IL-6蛋白水平（7278 ± 868.4 pg/g组织），表明局部炎症有所减轻。这一发现可以从A. paniculata提取物减少受感染仓鼠肺部组织学病变的能力中得到证明。此外，与对照组相比，两种提取物都能明显降低感染仓鼠外周血单核细胞中 IL-6 和 IP-10 mRNA 的表达，这表明提取物具有全身抗炎作用。总之，A. paniculata 提取物对 SARS-CoV-2 的潜在治疗作用源于其降低炎症细胞因子浓度和减轻肺部病理变化的能力。

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引用次数: 0

A formulation of combined Poria cocos and Cordyceps militaris rice ameliorates depressive-like effects by downregulating p38 MAPK signaling pathways 通过下调 p38 MAPK 信号通路，茯苓和冬虫夏草稻米复方制剂可改善抑郁样效应

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-16 DOI: 10.1016/j.jtcme.2024.05.002

Yun-Ju Huang , Hsin-Yu Wu , Wan-Ting Chang , Kuan-Hung Lu , Yu-En Lin , Huai-Syuan Huang , Yun-Sheng Lu , Lee-Yan Sheen

Background and aim

Poria cocos (PC) is traditional Chinese medicine with sedative, diuretic, and tonic properties. Cordyceps militaris rice (CMR) is a matrix used for the solid-state culture of C. militaris, which has been used for anti-angiogenetic and anti-inflammation effects. To investigate a formulation of combined Poria cocos and Cordyceps militaris rice (PC-CMR) alleviates anxiety behaviours and depressive-like effects for the first time.

Experimental procedure

After five-week of stress, rats were induced by unpredictable chronic mild stress (UCMS) treated with PC-CMR formulation. Then, rats underwent various behavior tests, testing serum inflammatory mediators by enzyme-linked immunosorbent assays (ELISA), evaluated monoamines by high-performance liquid chromatography (HPLC) analysis, and protein expression by western blotting.

Results

Treatment with doses of formulated PC-CMR reversed sucrose preference and increased amount of time spent on the open arms in rat behavioral tests. The formulated PC-CMR significantly reduced glutamate content and turnover rates of serotonin and dopamine in the prefrontal cortex of rats. In the inflammation-related pathway, PC-CMR decreased interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in serum and downregulated the expression of p38 mitogen-activated protein kinase (p38 MAPK), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the prefrontal cortex and signal transducer and activator of transcription 3 (STAT3), and indoleamine 2,3-dioxygenase (IDO) in the amygdala.

Conclusion

Our findings suggest that the formulated PC-CMR could have antidepressive and anxiolytic effects by modulating neurotransmitter levels and reducing inflammation.

背景：可可豆（PC）是一种具有镇静、利尿和滋补作用的中药。蛹虫草（Cordyceps militaris rice， CMR）是用于蛹虫草固态培养的基质，具有抗血管生成和抗炎症作用。首次研究茯苓蛹虫草米复合制剂对焦虑行为和抑郁样效应的缓解作用。实验方法应激5周后，用PC-CMR制剂诱导大鼠出现不可预测的慢性轻度应激（UCMS）。然后，对大鼠进行各种行为测试，用酶联免疫吸附法（ELISA）检测血清炎症介质，用高效液相色谱法（HPLC）分析单胺，用免疫印迹法（western blotting）检测蛋白质表达。结果配制的PC-CMR剂量逆转了大鼠对蔗糖的偏好，并增加了大鼠行为测试中张开双臂的时间。配制的PC-CMR显著降低大鼠前额皮质谷氨酸含量和血清素和多巴胺的周转率。在炎症相关通路中，PC-CMR降低血清白细胞介素-1β (IL-1β)和肿瘤坏死因子-α (TNF-α)水平，下调前额叶皮层p38丝裂原活化蛋白激酶（p38 MAPK）、活化B细胞核因子κB轻链增强子（NF-κB）和杏仁核信号传导和转录激活因子3 （STAT3）、吲哚胺2,3-双加氧酶（IDO）的表达。结论复方PC-CMR可能通过调节神经递质水平和减轻炎症而具有抗抑郁和抗焦虑作用。

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引用次数: 0

Lotus seed (Nelumbo nucifera Gaertn.) extract at low dose ameliorates reproductive dysfunction in l-NAME-induced hypertension and oxidative stress in male rats 低剂量莲子（Nelumbo nucifera Gaertn.）提取物可改善 L-NAME 诱导的高血压和氧化应激导致的雄性大鼠生殖功能障碍

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-04 DOI: 10.1016/j.jtcme.2024.05.001

Anjaree Inchan , Krongkarn Chootip , Kitiya Kongthong , Tippaporn Bualeong , Wiriyaporn Sumsakul , Phapada Apaikawee , Pakaporn Sa-Nguanpong , Sinlapachai Senarat , Jutamas Wongphoom , Natthawut Charoenphon

Background and aim

Nelumbo nucifera Gaertn. (Lotus) seeds have been traditionally used to treat hypertension and are reported to have antioxidant effects. The role of lotus seeds in reproductive dysfunction associated with hypertension has not yet been investigated. Therefore, we aimed to evaluate lotus seed extract (LSE) on reproductive functions in N^ω-nitro-l-arginine methyl ester hydrochloride (l-NAME) induced hypertension and oxidative stress in rats.

Experimental procedure

Male Sprague–Dawley rats were allocated into six groups: i) normotensive rats, ii) rats treated with l-NAME (40 mg/kg), iii-vi) rats treated with l-NAME + either 10, 100, 300 mg/kg LSE or 5 mg/kg captopril for 5 weeks. Systolic blood pressure (SBP), sperm concentrations, viability and motility and the serum testosterone were measured. The morphology, malondialdehyde (MDA) and superoxide dismutase (SOD) activities were evaluated in testis and epididymis.

Results and conclusion

l-NAME-treated rats exhibited high SBP (181.9 ± 6.5 mmHg, P < 0.01) and reduced SOD activities in comparison to the normal group. These were significantly improved by the administration of 10, 100, 300 mg/kg LSE or captopril (P < 0.05). l-NAME also led to a significant increase in MDA, and reductions in sperm count, viability and motility, testosterone levels, as well as the diameter and epithelial thickness of the seminiferous tubules. Treatment with 10 mg/kg LSE or captopril significantly attenuated these effects (P < 0.05), whereas 100 and 300 mg/kg LSE showed no significant differences compared to the l-NAME group. Thus, our study demonstrates that a low dose of LSE has therapeutic potential in alleviating reproductive dysfunction associated with hypertension.

背景与目的。传统上，莲子被用来治疗高血压，据报道莲子具有抗氧化作用。莲子在高血压相关生殖功能障碍中的作用尚未被研究。因此，我们旨在评价莲子提取物（LSE）对ω-硝基-l-精氨酸甲酯盐化物（l-NAME）诱导的高血压和氧化应激大鼠生殖功能的影响。将雄性sd大鼠分为6组：i)正常血压大鼠，ii) l-NAME （40 mg/kg）大鼠，iii-vi) l-NAME + 10、100、300 mg/kg LSE或5 mg/kg卡托普利治疗5周。测定收缩压（SBP）、精子浓度、活力、活力及血清睾酮水平。测定睾丸和附睾的形态、丙二醛（MDA）和超氧化物歧化酶（SOD）活性。结果与结论：大鼠收缩压升高（181.9±6.5 mmHg）， P <；0.01)， SOD活性较正常组降低。服用10、100、300 mg/kg LSE或卡托普利(P <；0.05)。l-NAME还导致MDA显著增加，精子数量、活力和活力、睾酮水平以及精小管直径和上皮厚度降低。用10mg /kg LSE或卡托普利治疗可显著减弱这些效应(P <；0.05)，而100和300 mg/kg LSE组与l-NAME组相比差异不显著。因此，我们的研究表明，低剂量的LSE在缓解高血压相关的生殖功能障碍方面具有治疗潜力。

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引用次数: 0

Achillea extracts elicit anti-diabetic neuropathic pain by modulating inflammatory cytokines Achillea 提取物通过调节炎症细胞因子引发抗糖尿病神经病理性疼痛

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine

Pub Date : 2024-05-01 DOI: 10.1016/j.jtcme.2024.04.012

Ola Kbaydet , Maha Abou-Ela , Karim Raafat

Background and aim

The Eastern-Mediterranean herbal-remedies are utilized for their immunomodulatory-potentials. Achillea species have ethno-pharmacological significance. The current-work aims to perform an in-depth phytochemical-investigation on three Eastern-Mediterranean Achillea species, mainly, Achillea kotschyi Boiss. (AK), Achillea falcata L. (AF), and Achillea aleppica DC. (AAL) essential oils (EOs), and to investigate their antidiabetic, and antineuropathic pain, and inflammation, and to have insights into their mechanisms of action.

Experimental-procedure

The methods include EO-isolation from three different samples of each of the three species examined, optimization, and validation of GC-MS method, in-vivo antioxidant-technique, HbA1c diabetes-assessment, insulin-level, thermal-hyperalgesia and tactile-allodynia experiments, anti-inflammatory, and inflammatory-markers, TNF-α, IL-6, and 10 level monitoring.

Results and conclusion

The optimized and validated GC-MS method was developed to quantify the major EO components. The highest components detected on AK-EO were thujone (35.84 ± 0.01 %), α-phellandrene (15.99 ± 0.01), and ocimene (12.31 ± 0.01 %), while those of AF were ocimene (65.11 ± 0.01 %), thujone (14.68 ± 0.01 %), and resorcinol (5.00 ± 0.01 %), and the AAL-EO were m-toluamide (49.35 ± 0.01 %), eucalyptol (21.08 ± 0.01 %), and ocimene (13.48 ± 0.01 %). AAL-EO showed superiority in normalizing HbA1c-levels. AAL-EO showed the highest improvement in both thermal-hyperalgesia and tactile-allodynia latencies. The insulin-secretagogue-potential and the improvement of the antioxidant serum-catalase levels might be their mechanism of antinociception. AAL-EO highest-dose (300 mg/kg)showed superiority in the anti-inflammatory-potentials both acutely and chronically, compared to AK and AF-EOs. The decrease in the inflammatory-mediators, TNF-α and IL-6, and the improvement of IL-10 titers proved to be their mechanism of anti-inflammation. In conclusion, AK, AF, and AAL proved to be rich in essential-oil components that can ameliorate chronic-conditions like diabetes, diabetic-neuropathy pain, and inflammation.

背景和目的东地中海草药因其免疫调节潜力而被利用。水蛭属植物具有民族药理学意义。本研究旨在对东地中海地区的三种阿喀琉叶（Achillea kotschyi Boiss）进行深入的植物化学研究。（AK）、falcata L. （AF）和aleppica DC。（AAL）精油（EOs），并研究它们的抗糖尿病、抗神经性疼痛和炎症，并深入了解它们的作用机制。实验方法包括从三种被检测物种的三个不同样品中分离eo，优化和验证GC-MS方法，体内抗氧化技术，糖化血红蛋白糖尿病评估，胰岛素水平，热痛觉过敏和触觉异常性疼痛实验，抗炎和炎症标志物，TNF-α， IL-6和10水平监测。结果与结论建立了优化且有效的气相色谱-质谱定量方法。AK-EO中检测到的最高成分为图酮（35.84±0.01 %）、α-香檀烯（15.99±0.01 %）和辛美烯（12.31±0.01 %），AF中检测到的最高成分为辛美烯（65.11±0.01 %）、图美烯（14.68±0.01 %）和间苯二酚（5.00±0.01 %），AAL-EO中检测到的最高成分为间甲苯酰胺（49.35±0.01 %）、桉油醇（21.08±0.01 %）和辛美烯（13.48±0.01 %）。AAL-EO在使hba1c水平正常化方面具有优势。AAL-EO对热痛觉过敏和触觉异常性疼痛潜伏期的改善最大。胰岛素分泌潜能和抗氧化血清过氧化氢酶水平的提高可能是其抗伤机制。与AK和AF-EOs相比，AAL-EO最高剂量（300 mg/kg）在急性和慢性抗炎潜能方面均具有优势。炎症介质TNF-α和IL-6的降低和IL-10滴度的提高被证明是其抗炎机制。总之，AK， AF和AAL被证明含有丰富的精油成分，可以改善慢性疾病，如糖尿病，糖尿病神经病变疼痛和炎症。

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引用次数: 0