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Comparative efficacy of three synchronization protocols in anestrous goats (Capra hircus) 三种同步方案在发情山羊体内的效果比较
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.996
M. Shahzad, R. Kausar
The goal of this experiment was to compare the effectiveness of three popular synchronization protocols, viz. ovsynch, double prostaglandin (PGF2? /PG) injections, and MAP (Medroxyprogesterone acetate) sponges, in anestrous goats. Twenty one non pregnant multiparous anestrous goats with an average body condition score (BCS = 2.5) were selected. After the last PG injection, all the goats were exposed to three fertile bucks. They were observed to be in standing estrus. For further confirmation of copulation, a vaginal cytology test was performed for the presence of sperm inside the vaginal smear. Serum estradiol (E2) peaks were also estimated by using radioimmunoassay in estrus goats. MAP sponge efficiency with respect to estrus induction was found to be superior (57%) as compared to the rest (Ovsynch14 and PG 0%) (p< 0.05). Post PG standing estrus time in ovsynch and MAP groups was recorded as 48 h and 44 ± 12 h, respectively. The double PG group totally failed to show standing estrus. E2 peak levels ranging from 11-38 pg/ml in ovsynch and 10–25 pg/ml in the MAP group were observed in estrus goats. This study found the MAP sponge protocol most efficient for inducing estrus in anestrous goats.
本实验的目的是比较三种流行的同步协议的有效性,即ovsync,双前列腺素(PGF2?/PG)注射剂和MAP(醋酸甲孕酮)海绵。选取体况评分平均(BCS = 2.5)的非妊娠多产无病山羊21只。在最后一次注射PG后,所有山羊都接触了三只可育雄鹿。它们被观察到处于发情状态。为了进一步确认交配,阴道细胞学检查进行阴道涂片内精子的存在。用放射免疫法测定了发情山羊血清雌二醇(E2)峰值。MAP海绵诱导发情的效率(57%)高于其他(Ovsynch14和PG 0%) (p< 0.05)。ovsync组和MAP组PG后站立发情时间分别为48 h和44±12 h。双PG组完全没有出现持续发情。E2在发情山羊体内的峰值水平为11-38 pg/ml, MAP组为10-25 pg/ml。本研究发现MAP海绵方案最有效地诱导发情山羊。
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引用次数: 0
Discovery of large mangrove-dwelling Elysia species in the newly-grown mangrove habitats, Pattani, Thailand 在泰国北大年新生长的红树林栖息地中发现的大型栖息于红树林的Elysia物种
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.1002
Somsak Buatip, Supaporn Saengkeaw, Supakan Buatip
The first report on the occurrence of the remarkable and highly ephemeral sap-sucking sea slugs Elysia bangtawaensis and E. leucolegnote from the newly grown mangrove forest in the Prince of Songkla University, Pattani, Thailand. Elysia was surveyed by exploring from the inner part to the floor front zone of the mangrove area. The various sizes and numbers of E. bangtawaensis were clumped distribution in some microhabitats throughout the area, while E. leucolegnote was distributed in the floor front zone of the area. Both species have similar external morphological characters with conspecifics previously reported in Pattani Bay, Gulf of Thailand, Andaman Sea, and elsewhere. E. bangtawaensis showed a surprisingly larger size than previously reported. This discovery is important in identifying the changes in ecosystems within the area to support the diversity of organisms that will come to use the area in the future.
首次报道了泰国北大年宋卡王子大学新生长的红树林中显著且高度短暂的吸液海蛞蝓Elysia bangtawaensis和E. leucolegnote的出现。通过从红树林区域的内部到地板前区域的探索来调查Elysia。不同大小和数量的邦塔瓦沙蚕在整个区域的一些微生境中呈块状分布,而白叶沙蚕则分布在区域的底前区。这两个物种具有相似的外部形态特征,与以前在北大年湾、泰国湾、安达曼海和其他地方报道的同种异种相似。E. bangtawaensis的体型比之前报道的要大得多。这一发现对于确定该地区生态系统的变化非常重要,以支持未来将利用该地区的生物多样性。
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引用次数: 0
Challenges faced by beef cattle farmers in innovation adoption towards food security and sustainability: a narrative review 肉牛养殖户在采用创新以实现粮食安全和可持续性方面面临的挑战:叙述性回顾
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.1004
F. A. Abdullah, Mahazan Muhammad, Mohd Saifoul Zamzuri Noor, Syamsuriana Sidek, Tengku Halimatun Sa’adiah T. Abu Bakar, M. Jusoh
The agricultural industry in Malaysia remains a vital element in providing food and employment. Introducing innovation in beef cattle farming has brought new changes in the livestock sector. Hopefully, it can generate a high income among farmers and brighten the Malaysian economy. However, introducing innovations such as assisted reproduction technology, biosecurity and intensive rearing require more significant effort from all parties, including farmers, extension agents and the government. This paper aims to identify beef cattle farmers' challenges in adopting innovations to improve beef production. Process of identifying, screening and eligibility is essential in producing an extensive literature review paper. From these processes, nine articles were found as the most relevant for this review article. The financial problem, communication failure and inaccessible information were the main challenges faced by farmers and extension agents in implementing innovations. Nevertheless, cooperation from all parties can lead to an excellent distribution of knowledge and skills among farmers.
马来西亚的农业仍然是提供粮食和就业的重要因素。在肉牛养殖中引入创新给畜牧业带来了新的变化。希望它能给农民带来高收入,并照亮马来西亚的经济。然而,引进诸如辅助生殖技术、生物安全和集约化饲养等创新需要包括农民、推广机构和政府在内的各方作出更大的努力。本文旨在确定肉牛养殖户在采用创新技术提高牛肉生产方面面临的挑战。识别,筛选和资格的过程是必不可少的,以产生广泛的文献综述论文。从这些过程中,我们发现九篇文章与这篇综述文章最相关。资金问题、沟通失败和信息难以获取是农民和推广人员在实施创新时面临的主要挑战。然而,各方的合作可以使知识和技能在农民之间得到很好的分配。
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引用次数: 0
Factors Influencing Farmers' Readiness to Face Covid-19 Post-Movement Control Order Challenges in Peninsular Malaysia 影响马来西亚半岛农民准备应对Covid-19迁移后控制秩序挑战的因素
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.1008
Murni Azureen Mohd Pakri, J. Arif Shah, Nur Bahiah Mohamed Haris, N. Mazlan
The COVID-19 pandemic is a global health catastrophe that has had severe effects on the worldwide economy, both directly and indirectly, because of the spread of the virus. The food and agriculture industries are also experiencing the effects. The COVID-19 epidemic disrupted the regular delivery system of agricultural extension services and the delivery of agricultural produce to markets. The study aimed to determine farmers' readiness to address post-MCO challenges in technology, implementation, decision-making, and leadership. Following the COVID-19 rules of reducing close contact to minimize virus transmission, a structured questionnaire was delivered to farmers online (Google form) and face-to-face with the support of extension agents. The results show that the relationship between independent and dependent variables was positive. Also, according to the findings, decision-making is the most crucial element influencing farmers' readiness to tackle post-movement control order issues. According to researchers, farmers' abilities should be developed to overcome challenges in pandemic conditions.
COVID-19大流行是一场全球卫生灾难,由于该病毒的传播,直接和间接地对全球经济产生了严重影响。食品和农业行业也受到了影响。2019冠状病毒病疫情扰乱了农业推广服务的正常提供系统和农产品向市场的供应。该研究旨在确定农民在技术、实施、决策和领导方面应对mco后挑战的准备情况。根据COVID-19减少密切接触以最大限度地减少病毒传播的规则,在推广人员的支持下,在线(谷歌表格)和面对面向农民发放了结构化问卷。结果表明,自变量与因变量之间呈正相关关系。此外,根据研究结果,决策是影响农民准备解决迁移后控制秩序问题的最关键因素。据研究人员说,应该发展农民的能力来克服大流行条件下的挑战。
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引用次数: 0
Molecular detection of chicken astrovirus in broiler chicken, Malaysia 马来西亚肉鸡星形病毒的分子检测
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.1001
Muhammad Redzwan Sidik, S. A. Shohaimi, Faizul Fikri Mohd Yusop, B. L. Leow, Mohd Khairil Azhar Md Haris, G. H. Ong, Maizatul Zaimi, Mohammad Jihan Redzuan
The emergence of avian diseases can cause major economic problems due to production losses and mortality in domestic poultry. Astrovirus is frequently associated with enteric diseases in poultry, being isolated from cases of runting-stunting syndrome (RSS) of broiler chickens, poult enteritis complex (PEC), and poult enteritis mortality syndrome (PEMS) of turkeys. Avian astrovirus can be detected in chickens from both healthy and poorly performing flocks. In Malaysia, information and reports regarding chicken astrovirus (CAstV) in poultry are limited. The objective of this study is to perform a phylogenetic study on the avian astrovirus isolated from a suspected case in 2019 and to determine the subgroups of avian astrovirus strains that existed in Malaysia. Reverse Transcription Polymerase chain reaction (RT-PCR) was performed based on the partial ORF1b gene and the nucleotide sequence was analyzed. Phylogenetic analysis showed that this isolate was clustered together with CAstV strains from several strain from USA, Malaysia and others. Furthermore, the isolate from broiler chicken showed 97.2% to 99.4% of its nucleotide identity with isolates from the American strains, compared to the previously CAstv Malaysia strain, which shared 94.8% to 95%.Therefore, the current study provides important information on the epidemiology of CAstV and highlights the importance of control strategies against CAstV-infected poultry in Malaysia.
由于家禽的生产损失和死亡,禽类疾病的出现可造成重大的经济问题。星状病毒通常与家禽肠道疾病有关,从肉鸡的生长发育迟缓综合征(RSS)、家禽肠炎复合体(PEC)和火鸡的家禽肠炎死亡综合征(PEMS)病例中分离出来。禽星状病毒可在健康鸡群和表现不佳的鸡群中检测到。在马来西亚,关于家禽中的鸡星状病毒(CAstV)的信息和报告有限。本研究的目的是对2019年从疑似病例中分离的禽流感星状病毒进行系统发育研究,并确定马来西亚存在的禽流感星状病毒株的亚群。对ORF1b部分基因进行逆转录聚合酶链反应(RT-PCR),分析其核苷酸序列。系统发育分析表明,该分离株与来自美国、马来西亚等地的CAstV毒株聚集在一起。此外,肉鸡分离物与美国菌株的核苷酸同源性为97.2% ~ 99.4%,而先前的马来西亚菌株的核苷酸同源性为94.8% ~ 95%。因此,目前的研究提供了关于CAstV流行病学的重要信息,并强调了在马来西亚针对CAstV感染家禽的控制策略的重要性。
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引用次数: 1
A preliminary investigation of genetic diversity amongst Rusa timorensis in Tanjung Malim, Perak and Bilut Agro Farm, Pahang, Malaysia 霹雳州丹绒马林和马来西亚彭亨州Bilut农场的东帝汶沙鼠遗传多样性初步调查
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.994
Kumara Thevan Krishnan, N. I
A study on genetic diversity analysis was conducted on Rusa timorensis obtained from state of Perak and state of Pahang to investigate the level of genetic diversity occur and to compare the diversity amongst two R. timorensis breeders in Malaysia. A total of six (n=6) individual samples of R. timorensis were extracted from Tanjung Malim, Perak and Bilut Agro Farm, Pahang and amplified using mitochondria deoxyribonucleic acid (mtDNA) primers gene as a target molecular marker. The mtDNA region was amplified using a set of cytochrome b gene primers (5”AAACCA GAAAAGGAGAGCAAC3”;5”TCATCTAGGCATTTTCAGTGCC3”) and nucleotide sequence of the mtDNA cyt b was aligned by using MEGA Ver 7.0. The result indicated that the R. timorensis from Pahang has a low degree of variation (0.252) of genetic distance compared to, R. timorensis from Perak (0.696). The phylogenetic three analysis, indicated, R. timorensis from Pahang resulted the highest intra-specific relationship at 100% compared to, R. timorensis from Perak at 63% of intra-specific relationship. The results showed that the genetic diversity of, R. timorensis in Perak and Pahang is likely to decrease in the future. Therefore, future breeding program plan needs to be implemented to diversify the genetics of genus Rusa in Malaysia.
摘要对马来西亚霹雳州和彭亨州的红背沙鼠进行了遗传多样性分析,探讨了红背沙鼠的遗传多样性水平,并比较了马来西亚两个红背沙鼠的遗传多样性。以线粒体脱氧核糖核酸(mtDNA)引物基因为靶分子标记,从槟榔州丹绒马林和彭亨州Bilut Agro农场共提取了6个(n=6)个铁铁褐毛杆菌样本。使用细胞色素b基因引物(5“AAACCA GAAAAGGAGAGCAAC3”;5“TCATCTAGGCATTTTCAGTGCC3”)扩增mtDNA区域,使用MEGA Ver 7.0对mtDNA cyt b的核苷酸序列进行比对。结果表明,彭亨州滇鼠与霹雳州滇鼠的遗传距离变异程度较低(0.252)。系统发育分析表明,来自彭亨州的帝汶红毛鼠种内亲缘关系最高,为100%,而来自霹雳州的帝汶红毛鼠种内亲缘关系为63%。结果表明,在霹雳州和彭亨州,帝汶红毛鼠的遗传多样性在未来可能会下降。因此,需要实施未来的育种计划计划,以使马来西亚的Rusa属遗传多样化。
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引用次数: 0
The effects of edible chitosan coating on prolonging the shelf life of green chilies (Capsicum annuum L.) 食用壳聚糖涂层对青椒保鲜期的影响
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.990
Kirubagarani N. Arumugam, N. Md Zain
Green chillies (Capsicum annuum L.) are susceptible to pest attack, experiences water loss rapidly in a short time and also experiences chilling injury when stored at a cool temperature that leads to shorter shelf life. Therefore, this study was conducted to examine the effects of chitosan at different concentrations (0.5%, 1.0% and 2.0%) on the shelf life of green chilies stored at ambient temperature (27°C) for 21 days. The effectiveness of the chitosan treatments in extending green chilies’ shelf-life was evaluated by determining their physical and chemical qualities. Coated green chilies are able to preserve for a longer period of time compared to uncoated green chilies. Regardless of any chitosan concentration, the total soluble solid content (TSS) of coated green chilies had increased starting from day 3 to day 21 day as compared to the control. At the highest concentration of 2.0%, chitosan treatment was able to reduce the pH by 28% on day 6 of the storage period, meanwhile, the pH was maintained across the concentration at the end of the storage period (day 21). It was found that the green chilies faced 10 to 22% weight loss across the concentration as compared to the control at the end of the storage period, where the highest weight loss was prominent at 2.0% chitosan. However, in terms of firmness, the chitosan treatments do not give any significant effect on all the treated green chilies. These results suggest that the application of edible chitosan coating contributes to lower pH and weight loss which is responsible for the longer shelf life of the green chilies.
绿辣椒(Capsicum annuum L.)易受害虫侵袭,在短时间内经历水分迅速流失,并且在低温下储存时也会经历冷害,导致保质期缩短。因此,本研究考察了不同浓度(0.5%、1.0%和2.0%)的壳聚糖对绿辣椒常温(27℃)保存21天保质期的影响。通过对青椒理化性状的测定,评价壳聚糖处理对青椒保鲜期的影响。包衣的青椒比没有包衣的青椒保存的时间更长。无论壳聚糖浓度如何,包衣青椒的总可溶性固形物含量(TSS)从第3天开始到第21天与对照相比有所增加。在最高浓度为2.0%时,壳聚糖处理能使贮藏第6天的pH降低28%,而在贮藏结束时(第21天),整个浓度的pH都保持不变。结果发现,与对照组相比,不同浓度的绿辣椒在储存期结束时体重减轻了10%至22%,其中2.0%壳聚糖的重量减轻幅度最大。然而,就紧致度而言,壳聚糖处理并没有对所有处理过的青椒产生任何显著影响。综上所述,食用壳聚糖涂层的应用降低了青椒的pH值,减轻了青椒的重量,从而延长了青椒的保质期。
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引用次数: 0
Prenatal zestoretic exposure ameliorates developmental landmarks in rat offspring 产前孕激素暴露可改善大鼠后代的发育标志
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.993
N. P, Srivedi V, Ramachandra Reddy P
Zestoretic, a combination of lisinopril and hydrochlorothiozide used as anti-hypertension drug prescribed even during pregnancy. Role of zestoretic on the developmental landmars of young ones exposed prenatally are yet to be established. Hence, the present investigation initiated to test in Wistar rats. Three groups of inseminated female rats were oral gavaged with zestoretic (lisinopril varied concentrations + hydrochlorothiozide 12.5mg) 25, 50 and 100 mg/Kg body weight (BW) on gestation days 7, 9, 11 and 13, whereas control group with double distilled water. Clinical toxicity found in few dams from 50 and 100 mg/Kg BW received rat shown aggressive behavior during experimentation. The developmental landmarks, such as pinna attachment, ear opening, fur development, eye opening, upper and lower incisor eruption, crown rump length, vaginal opening and testes descend were measured periodically on their postnatal days in all the young ones. The number of live pups in zestoretic 50, 100 mg/Kg BW administered females are significantly less (P
Zestoretic,赖诺普利和氢氯噻嗪的混合物,用作抗高血压药,即使在怀孕期间也可以开处方。孕激素在产前暴露的幼体发育过程中的作用尚未确定。因此,本研究开始在Wistar大鼠中进行试验。在妊娠第7、9、11、13天,三组授精雌性大鼠分别灌胃不同浓度赖诺普利+氢氯噻嗪12.5mg的zestoretic 25、50、100 mg/Kg体重(BW),对照组灌胃双蒸馏水。临床毒性在50和100 mg/Kg体重的小鼠中发现,实验期间小鼠表现出攻击行为。在出生后定期测定各组幼犬耳廓附着、耳开口、皮毛发育、睁眼、上、下切牙萌出、冠臀长、阴道开口、睾丸下降等发育指标。雌鼠在饲喂50、100 mg/Kg BW时,其活仔数显著低于对照组(P < 0.05)
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引用次数: 0
The emerging evidences related to the Inter-relationship between COVID-19 and climate change: A scoping review perspective 关于COVID-19与气候变化之间相互关系的新证据:范围审查视角
Pub Date : 2022-12-30 DOI: 10.47253/jtrss.v10i2.1006
Hayrol Azril Mohamed Shaffril, Asnarulkhadi Abu Samah, Samsul Farid Samsuddin, Hamizah Sahharon
Coronavirus Disease 2019 (COVID-19) has affected a number of integral aspects and climate change is one of them. The inter-relationship between COVID-19 and climate change has garnered the interest among scholars across the globe to probe into the related emerging issues. With the escalating number of published articles, a pressing need is present to map and review all available studies in order to capture comprehensive and organised views for future scholars to draw upon the existing pattern of the interchanging correlation between COVID-19 and climate change. This scoping review is guided by a primary research question - what is the inter-relationship between COVID-19 and climate change. Related articles and documents were retrieved from Scopus, Science Direct, and Google Scholars databases. The thematically analysis data were classified into four emerging themes: (1) reduced air pollution, (2) low carbon emission, (3) the impact of temperature and humidity variation on COVID-19, and (4) the impact of air pollution on COVID-19. Variances in the outcomes, particularly on the effects of temperature, humidity, and polluted air on COVID-19, suggest further exploration endeavours. Essentially, the economic impact exerted by COVID-19 and its overall effect on budget to combat climate change remain untapped.
2019冠状病毒病(COVID-19)影响了许多整体方面,气候变化是其中之一。COVID-19与气候变化之间的相互关系引起了全球学者的兴趣,并开始探讨相关的新兴问题。随着发表的文章数量不断增加,目前迫切需要绘制和审查所有现有的研究,以便为未来的学者捕获全面和有组织的观点,以利用COVID-19与气候变化之间相互关联的现有模式。本次范围审查以一个主要研究问题为指导,即COVID-19与气候变化之间的相互关系是什么。相关文章和文献检索自Scopus、Science Direct和谷歌Scholars数据库。主题分析数据被分为四个新兴主题:(1)减少空气污染,(2)低碳排放,(3)温湿度变化对COVID-19的影响,(4)空气污染对COVID-19的影响。结果的差异,特别是温度、湿度和污染空气对COVID-19的影响,表明需要进一步探索。从本质上讲,COVID-19造成的经济影响及其对应对气候变化预算的总体影响仍未得到充分利用。
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引用次数: 0
Combination of stabiliser and emulsifier for Pink Guava Juice Drink (PGJD) fortified with Vitamin E 添加维生素E的粉红番石榴果汁饮料的稳定剂和乳化剂的组合
Pub Date : 2022-06-30 DOI: 10.47253/jtrss.v10i1.899
M. S. Hashim, Faikah Awang @ Ismail
In the beverages industry, the stabilizer and emulsifier were used to enhance the quality of Pink Guava Juice Drink (PGJD) in terms of colour, taste, and texture. Thus, the product is favourable and marketable. This study was conducted to find the best emulsifier combination for PGJD. The combination of six different stabilizers such as Guar gum (GG), Carboxyl methyl cellulose (CMC), Arabic gum (AG), Xanthan gum (XG), Propylene glycol alginate (PGA), and Pectin were applied to PGJD formulation. Meanwhile, about three emulsifiers which are Arabic gum (AG), Polysorbate 80 (P80), and Propylene glycol alginate (PGA) were used to emulsify vitamin E in PGJD. The ratio for both stabilizers and emulsifiers varied accordingly. The most stable and suitable combination of stabilizers and emulsifiers was later added to PGJD. The combination of XG and CMC at the concentration of 70:30 with 0.2% (w/v) was the best stabilizer. Meanwhile, P80 was found to be the best emulsifier at a concentration of 0.8% (w/v) and PGJD fortified with 225 mg of vitamin E was chosen as the most acceptable PGJ among panelists. This finding of the present study can be a guideline for beverage manufacturers in selecting the best stabilizers and emulsifiers.
在饮料工业中,使用稳定剂和乳化剂来提高粉红番石榴汁饮料的色、味、质质量。因此,产品是有利的和适销对路的。本研究旨在寻找治疗PGJD的最佳乳化剂组合。将瓜尔胶(GG)、羧甲基纤维素(CMC)、阿拉伯胶(AG)、黄原胶(XG)、海藻酸丙二醇酯(PGA)、果胶等6种稳定剂组合应用于PGJD配方中。同时,用阿拉伯树胶(AG)、聚山梨酯80 (P80)和海藻酸丙二醇酯(PGA)三种乳化剂乳化PGJD中的维生素E。稳定剂和乳化剂的比例也相应变化。然后在PGJD中加入最稳定、最合适的稳定剂和乳化剂组合。XG与CMC以0.2% (w/v)、70:30的浓度组合为最佳稳定剂。同时,在乳化剂浓度为0.8% (w/v)时,P80是最佳乳化剂,并选择添加225 mg维生素E的PGJD作为最可接受的乳化剂。本研究结果可为饮料生产企业选择最佳的稳定剂和乳化剂提供指导。
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引用次数: 0
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Journal of Tropical Resources and Sustainable Science (JTRSS)
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